Publications

prevalence of arcobacter butzleri in the stool samples received in a tertiary hospital in singapore: is it necessary to screen for it?		2015	26308127
prevalence of ten putative virulence genes in the emerging foodborne pathogen arcobacter isolated from food products.	arcobacter spp. are considered to be emerging food- and waterborne pathogens for both humans and animals. however, their virulence mechanisms are still poorly understood. in this study the presence of ten virulence genes (cadf, ciab, cj1349, heca, hecb, mvin, plda, irga, tlya and iroe) was assessed in a set of 47 strains of arcobacter butzleri, 10 of arcobacter cryaerophilus and 1 arcobacter skirrowii strain recovered from different food products (pork, chicken, beef, milk, clams and mussels). o ...	2015	26338128
tracing enteric pathogen contamination in sub-saharan african groundwater.	quantitative pcr (qpcr) can rapidly screen for an array of faecally-derived bacteria, which can be employed as tracers to understand groundwater vulnerability to faecal contamination. a microbial dna qpcr array was used to examine 45 bacterial targets, potentially relating to enteric pathogens, in 22 groundwater supplies beneath the city of kabwe, zambia in both the dry and subsequent wet season. thermotolerant (faecal) coliforms, sanitary risks, and tryptophan-like fluorescence, an emerging rea ...	2015	26363144
arcobacter butzleri induce colonic, extra-intestinal and systemic inflammatory responses in gnotobiotic il-10 deficient mice in a strain-dependent manner.	the immunopathological impact of human arcobacter (a.) infections is under current debate. episodes of gastroenteritis with abdominal pain and acute or prolonged watery diarrhea were reported for a. butzleri infected patients. whereas adhesive, invasive and cytotoxic capacities have been described for a. butzleri in vitro, only limited information is available about the immunopathogenic potential and mechanisms of infection in vivo.	2015	26406497
[detection of biofilm formation by selected pathogens relevant to the food industry].	detection of biofilm formation by microbial pathogens relevant to the food industry and comparison of biofilm formation under different conditions of culture.	2015	26448305
multilocus sequence typing of arcobacter butzleri isolates collected from dairy plants and their products, and comparison with their pfge types.	the present study aimed to determine, by multilocus sequence type (mlst), the heterogeneity level of arcobacter butzleri isolates and to compare mlst and pulsed-field gel electrophoresis (pfge) in terms of discriminatory power (di) as well as unidirectional and bi-directional concordance.	2016	26481316
arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models.	the pathogenic potential of arcobacter butzleri isolates on human (ht-29/b6) and porcine epithelial (ipec-j2) cells was investigated by in vitro assays.	2016	26481610
survey of small intestinal and systemic immune responses following murine arcobacter butzleri infection.	arcobacter (a.) butzleri has been described as causative agent for sporadic cases of human gastroenteritis with abdominal pain and acute or prolonged watery diarrhea. in vitro studies revealed distinct adhesive, invasive and cytotoxic properties of a. butzleri. information about the underlying immunopathological mechanisms of infection in vivo, however, are scarce. the aim of this study was to investigate the immunopathological properties of two different a. butzleri strains in a well-establishe ...	2015	26483849
genetic diversity and incidence of virulence-associated genes of arcobacter butzleri and arcobacter cryaerophilus isolates from pork, beef, and chicken meat in poland.	incidence of 9 virulence-associated genes and genetic diversity was determined in 79 a. butzleri and 6 a. cryaerophilus isolates from pork, beef, and chicken meat. all a. butzleri isolates harboured the tlya gene, and most of them carried ciab, mvin, plda, cadf, and cj1349 genes. ciab was found to occur with higher frequency in poultry if compared with pork (p = 0.0007), while irga was more frequent in poultry than in beef (p = 0.007). all 6 a. cryaerophilus isolates harboured the ciab gene, whi ...	2015	26539546
toll-like receptor-4 is essential for arcobacter butzleri-induced colonic and systemic immune responses in gnotobiotic il-10(-/-) mice.	arcobacter butzleri causes sporadic cases of gastroenteritis, but the underlying immunopathological mechanisms of infection are unknown. we have recently demonstrated that a. butzleri-infected gnotobiotic il-10(-/-) mice were clinically unaffected but exhibited intestinal and systemic inflammatory immune responses. for the first time, we here investigated the role of toll-like receptor (tlr)-4, the main receptor for lipopolysaccharide and lipooligosaccharide of gram-negative bacteria, in murine ...	2015	26716021
toll-like receptor-4 dependent small intestinal immune responses following murine arcobacter butzleri infection.	sporadic cases of gastroenteritis have been attributed to arcobacter butzleri infection, but information about the underlying immunopathological mechanisms is scarce. we have recently shown that experimental a. butzleri infection induces intestinal, extraintestinal and systemic immune responses in gnotobiotic il-10(-/-) mice. the aim of the present study was to investigate the immunopathological role of toll-like receptor-4, the receptor for lipopolysaccharide and lipooligosaccharide of gram-neg ...	2015	26716022
development and evaluation of multiplex pcr assays for rapid detection of virulence-associated genes in arcobacter species.	as the pathogenicity of arcobacter species might be associated with various virulence factors, this study was aimed to develop and optimize three single-tube multiplex pcr (mpcr) assays that can efficiently detect multiple virulence-associated genes (vags) in arcobacter spp. including the arcobacter butzleri, arcobacter cryaerophilus and arcobacter skirrowii, respectively. the recognized target virulence factors used in the study were fibronectin binding protein (cj1349), filamentous hemagglutin ...	2016	26769558
antimicrobial susceptibility testing of arcobacter butzleri and arcobacter cryaerophilus strains isolated from belgian patients.	to evaluate the feasibility of different methods for susceptibility testing of human arcobacter isolates, to assess susceptibility to antibiotics commonly used to treat diarrhoeal illness and to obtain mic distribution data.	2016	26851610
comparative detection and quantification of arcobacter butzleri in stools from diarrheic and nondiarrheic people in southwestern alberta, canada.	arcobacter butzleri has been linked to enteric disease in humans, but its pathogenicity and epidemiology remain poorly understood. the lack of suitable detection methods is a major limitation. using comparative genome analysis, we developed pcr primers for direct detection and quantification ofa. butzleri dna in microbiologically complex matrices. these primers, along with existing molecular and culture-based methods, were used to detecta. butzleri and enteric pathogens in stools of diarrheic an ...	2016	26865686
arcobacter butzleri survives within trophozoite of acanthamoeba castellanii.	the survival of three arcobacter butzleri strains inside acanthamoeba castellanii was assessed using axenic cultures of a. castellanii that were inoculated with the tested strains and incubated at 26°c under aerobic conditions for 240h. the behavior of bacteria in contact with amoebae was monitored using phase contrast microscopy. the bacterial survival rate within amoebae was assessed through counting colony forming units, using the gentamicin protection assay. all a. butzleri strains were able ...	2017	26972277
survival capacity of arcobacter butzleri inoculated in poultry meat at two different refrigeration temperatures.	arcobacter spp. are emerging enteropathogens and potential zoonotic agents that can be transmitted by food and water, being considered a public health risk. the high isolation rate of these bacteria from poultry products suggests that it may be a major source of human infections. one hallmark for differentiating the genus arcobacter from campylobacter includes their growing capacity at low temperatures (15-30 °c) under aerobic conditions. however, little is known about the population density var ...	2016	27007565
occurrence of potentially pathogenic arcobacters in shellfish.	considering that several recent cases of human gastroenteritis have been associated with species from the arcobacter genus, and that few data are currently available about the occurrence of this genus in italian shellfish, the aim of the present study was to evaluate the occurrence of arcobacter spp. and the presence of virulence-associated genes. the approach consisted of cultural and biomolecular (multiplex-pcr and 16s-rflp) methods identifying isolates, followed by pcr assays aimed at the cad ...	2016	27052698
isolation and identification of arcobacter species from environmental and drinking water samples.	water plays an important role in the transmission of arcobacter spp. to animals and humans. the aim of this study was to isolate and characterize arcobacter spp. from 115 different water samples (66 sewage, 25 rivers, 16 spring water, and 8 drinking water) in izmir, turkey. in total, 41 samples (35.7 %) were found positive for arcobacter spp. by the genus-specific pcr. arcobacter butzleri was detected in 39 out of 115 samples (33.9 %) including 24 sewage, 13 rivers, and 2 spring water. the remai ...	2016	27106697
intestinal expression of genes encoding inflammatory mediators and gelatinases during arcobacter butzleri infection of gnotobiotic il-10 deficient mice.	we have previously shown that arcobacter butzleri induces intestinal, extra-intestinal, and systemic immune responses in perorally infected gnotobiotic il-10(-/-) mice in a strain-dependent fashion. here, we present a comprehensive survey of small and large intestinal expression profiles of inflammatory and regulatory mediators as well as of the matrix-degrading gelatinases mmp-2 and mmp-9 following murine a. butzleri infection. gnotobiotic il-10(-/-) mice were infected with a. butzleri strains ...	2016	27141315
toll-like receptor-4 dependent intestinal gene expression during arcobacter butzleri infection of gnotobiotic il-10 deficient mice.	we have previously shown that arcobacter butzleri infection induces toll-like receptor (tlr) -4 dependent immune responses in perorally infected gnotobiotic il-10(-/-) mice. here, we analyzed tlr-4-dependent expression of genes encoding inflammatory mediators and matrix-degrading gelatinases mmp-2 and -9 in the small and large intestines of gnotobiotic tlr-4-deficient il-10(-/-) mice that were perorally infected with a. butzleri strains ccug 30485 or c1, of human and chicken origin, respectively ...	2016	27141316
the first experimental research on the pathogenicity of arcobacter butzleri in zebrafish.	this experimental study was conducted to investigate the pathogenicity and histopathology of arcobacter butzleri in zebrafish model organism. firstly, mean infective dose (id50) of a. butzleri was calculated in zebrafish as 1.3×10(8)cfu/ml and 1×10(5)cfu/ml by immersion and intraperitoneal injection, respectively. for histopathological trials, the id50 of a. butzleri were given to zebrafish by both immersion and intraperitoneal routes and then, clinical and pathological findings were evaluated o ...	2016	27259824
isolation and characterization of arcobacter spp. from fresh seafood and the aquatic environment.	arcobacter is an emerging pathogen associated with foods of animal origin. members of the genus arcobacter are increasingly being isolated from fish, shellfish and the aquatic environment. in the present study, we analyzed fish, shellfish and water samples for the presence of arcobacter spp. by conventional isolation as well as by direct pcr on the enrichment broth. of 100 samples comprising of 42 finfish, 34 shellfish and 24 water samples analyzed, arcobacter spp. was isolated from 8 (19%) finf ...	2016	27261768
metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water.	the presence of pathogenic bacteria and the dissemination of antibiotic resistance genes (args) may pose big risks to the rivers that receive the effluent from municipal wastewater treatment plants (wwtps). in this study, we investigated the changes of bacterial community and args along treatment processes of one wwtp, and examined the effects of the effluent discharge on the bacterial community and args in the receiving river. pyrosequencing was applied to reveal bacterial community composition ...	2016	27340885
microrna response of primary human macrophages to arcobacter butzleri infection.	the role of micrornas (mirnas) in infectious diseases is becoming more and more apparent, and the use of mirnas as a diagnostic tool and their therapeutic application has become the major focus of investigation. the aim of this study was to identify mirnas involved in the immune signaling of macrophages in response to arcobacter (a.) butzleri infection, an emerging foodborne pathogen causing gastroenteritis. therefore, primary human macrophages were isolated and infected, and mirna expression wa ...	2016	27429792
the immunopathogenic potential of arcobacter butzleri - lessons from a meta-analysis of murine infection studies.	only limited information is available about the immunopathogenic properties of arcobacter infection in vivo. therefore, we performed a meta-analysis of published data in murine infection models to compare the pathogenic potential of arcobacter butzleri with campylobacter jejuni and commensal escherichia coli as pathogenic and harmless reference bacteria, respectively.	2016	27438014
occurrence of emerging food-borne pathogenic arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables.	given that changes in consumer food behaviours have led to an increase in the demand for pre-cut ready-to-eat (rte) vegetables, and that few data are currently available on the occurrence of arcobacter spp. in such foods, the aim of the present study was to assess the occurrence of arcobacter spp. that carry virulence-associated genes on pre-cut rte vegetables, using cultural and molecular methods. arcobacter was detected using biomolecular identification methods in 44/160 (27.5%) of the samples ...	2016	27442848
efficacy of wastewater treatment on arcobacter butzleri density and strain diversity.	arcobacter butzleri is a suspected waterborne enteric pathogen that is ubiquitous in the environment, but the degree to which wastewater treatment prevents entry of a. butzleri into environmental waters and the risks posed are not well established. untreated and treated wastewater samples (n = 260) were collected weekly from the lethbridge and fort macleod wastewater treatment facilities (the two major municipal inputs in southwestern alberta, canada) from may 2008 to april 2009. untreated waste ...	2016	27636152
genetic diversity of arcobacter isolated from bivalves of adriatic and their interactions with mytilus galloprovincialis hemocytes.	the human food-borne pathogens arcobacter butzleri and a. cryaerophilus have been frequently isolated from the intestinal tracts and fecal samples of different farm animals and, after excretion, these microorganisms can contaminate the environment, including the aquatic one. in this regard, a. butzleri and a. cryaerophilus have been detected in seawater and bivalves of coastal areas which are affected by fecal contamination. the capability of bivalve hemocytes to interact with bacteria has been ...	2017	27650799
unique and universal features of epsilonproteobacterial origins of chromosome replication and dnaa-dnaa box interactions.	in bacteria, chromosome replication is initiated by the interaction of the initiator protein dnaa with a defined region of a chromosome at which dna replication starts (oric). while dnaa proteins share significant homology regardless of phylogeny, oric regions exhibit more variable structures. the general architecture of orics is universal, i.e., they are composed of a cluster of dnaa binding sites, a dna-unwinding element, and sequences that bind regulatory proteins. however, detailed structure ...	2016	27746772
detection of arcobacter spp. in mytilus galloprovincialis samples collected from apulia region.	the aim of the study was to evaluate the occurrence of arcobacter spp. in 20 samples of mytilus galloprovincialis purchased at fish markets in apulia region. the detection of arcobacter spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mccd) agar supplemented with cefoperazone, amphotericin b and teicoplanin (cat). in 6 out of the 20 tested samples the presence of arcobacter spp. was found and confirmed by genus-based polymerase chain reaction. all t ...	2015	27800382
isolation of campylobacter spp. from client-owned dogs and cats, and retail raw meat pet food in the manawatu, new zealand.	campylobacter causes acute gastroenteritis in people worldwide and is frequently isolated from food, animals and the environment. the disease is predominately food-borne but many routes of transmission and sources of infection have been described, including contact with pets. the prevalence of campylobacter spp. in dogs and cats varies widely, and data on new zealand pets are limited. this study aimed to investigate the prevalence of campylobacter spp. in dogs, cats and retail raw meat pet food ...	2016	27860343
the use of two culturing methods in parallel reveals a high prevalence and diversity of arcobacter spp. in a wastewater treatment plant.	the genus arcobacter includes species considered emerging food and waterborne pathogens. despite arcobacter has been linked to the presence of faecal pollution, few studies have investigated its prevalence in wastewater, and the only isolated species were arcobacter butzleri and arcobacter cryaerophilus. this study aimed to establish the prevalence of arcobacter spp. at a wwtp using in parallel two culturing methods (direct plating and culturing after enrichment) and a direct detection by m-pcr. ...	2016	27981053
occurrence of putative virulence genes on arcobacter butzleri isolated from three different environmental sites throughout the dairy chain.	this comparative study investigated the occurrence of cadf, cj1349, ciab, plda, tlya, heca, hecb, mvin, irga and iroe genes in 212 arcobacter butzleri isolated from three different environmental sites linked to the dairy chain (farms, industrial and artisanal dairy plants) located in three italian regions (lombardy, emilia-romagna and calabria).	2017	28106302
detection, identification, and antimicrobial susceptibility of arcobacter spp. isolated from shellfish in spain.	this work aimed to determine the presence of arcobacter spp. in shellfish and to determine its susceptibility to quinolones. one hundred samples (41 mussels, 37 clams, and 22 cockles) were purchased from different local retail shops in valencia, spain, from september 2013 to june 2015. all samples were analyzed simultaneously by culture, after an enrichment step, and by polymerase chain reaction (pcr), directly and after enrichment. the susceptibility to levofloxacin and ciprofloxacin of the iso ...	2017	28121468
[arcobacter butzleri].		2016	28146191
prevalence and diversity of waterborne arcobacter butzleri in southwestern alberta, canada.	arcobacter butzleri is a potential enteric pathogen to human beings, but its reservoirs and modes of transmission are largely unverified. microbiological and molecular detection and subtyping techniques can facilitate surveillance of a. butzleri in hosts and environmental reservoirs. we isolated a. butzleri from 173 surface water samples (25.6%) and 81 treated wastewater samples (77.9%) collected in southwestern alberta over a 1-year period. arcobacter butzleri isolates (n = 500) were genotyped ...	2017	28177789
arcobacter spp. in fecal samples from brazilian farmed caimans (caiman yacare, daudin 1802).	the aim of this study was to perform the identification and molecular characterization of arcobacter cryaerophilus and arcobacter butzleri isolated from caiman (caiman yacare), kept at a production farm, in brazil. forty fecal samples were analyzed. after isolation and identification, 21/40 strains of a. butzleri and 19/40 strains of a. cryaerophilus were subjected to pcr for potential virulence gene detection. the results of the pcr showed 38/40 strains positive for the cadf, cj1349, ciab, and ...	2017	28321791
study of biofilm formation ability of foodborne arcobacter butzleri under different conditions.	the transmission of arcobacter butzleri , an emerging food- and waterborne pathogen, is possibly favored by its ability to adhere to abiotic surfaces. in this study, we assessed the biofilm formation ability of 42 a. butzleri isolates recovered from different food products. overall, nine isolates (21.4%) were able to adhere to polystyrene. among them, a chicken-derived isolate was classified as strongly adherent. based on the chi-square test, no relation was found between the adhesive abilities ...	2017	28358260
comparative isolation and genetic diversity of arcobacter sp. from fish and the coastal environment.	arcobacter species are emerging food-borne and water-borne human pathogens associated mostly with food animals and their environment. the present study was aimed to isolate arcobacter species from fish, shellfish and coastal water samples using two methods and to determine their genetic diversity. of 201 samples of fish, shellfish and water samples analysed, 66 (32·8%) samples showed the presence of arcobacter dna from both arcobacter enrichment broth and bolton broth. arcobacters were isolated ...	2017	28394467
susceptibility to 18 drugs and multidrug resistance of arcobacter isolates from different sources within the czech republic.	arcobacter spp. are considered to be potential foodborne pathogens, and consumption of contaminated food containing these bacteria could endanger human and animal health. arcobacter butzleri and arcobacter cryaerophilus are the species most frequently isolated from food of animal origin and from other samples. the aim of this study was to evaluate the susceptibility of arcobacters isolated in the czech republic. no information about antibiotic susceptibility and multidrug resistance of arcobacte ...	2017	28400212
functionalized fullerene targeting human voltage-gated sodium channel, hnav1.7.	mutations of hnav1.7 that cause its activities to be enhanced contribute to severe neuropathic pain. only a small number of hnav1.7 specific inhibitors have been identified, most of which interact with the voltage-sensing domain of the voltage-activated sodium ion channel. in our previous computational study, we demonstrated that a [lys6]-c84 fullerene binds tightly (affinity of 46 nm) to navab, the voltage-gated sodium channel from the bacterium arcobacter butzleri. here, we extend this work an ...	2017	28586206
application of long amplicon propidium monoazide-pcr to assess the effects of temperature and background microbiota on pathogens in river water.	the decay rates of enteric waterborne pathogens were evaluated following the introduction of yersinia enterocolitica, salmonella enterica, campylobacter jejuni and arcobacter butzleri into river water at different temperatures (5, 15 and 25°c) for a period of 28 days. to improve the accuracy of the results a molecular viability assay, long amplicon propidium monoazide-polymerase chain reaction (pma-pcr), was used to quantify the viable cell concentration and results from pcr with and without pma ...	2017	28598346
characterization of the emerging zoonotic pathogen arcobacter thereius by whole genome sequencing and comparative genomics.	four arcobacter species have been associated with human disease, and based on current knowledge, these gram negative bacteria are considered as potential food and waterborne zoonotic pathogens. at present, only the genome of the species arcobacter butzleri has been analysed, and still little is known about their physiology and genetics. the species arcobacter thereius has first been isolated from tissue of aborted piglets, duck and pig faeces, and recently from stool of human patients with enter ...	2017	28671965
virulence gene profiles of arcobacter species isolated from animals, foods of animal origin, and humans in andhra pradesh, india.	this study aimed to detect putative virulence genes in arcobacter species of animal and human origin.	2017	28717327
fate of antibiotic resistance genes and their associations with bacterial community in livestock breeding wastewater and its receiving river water.	large amounts of antibiotics are currently used in livestock breeding, which is the main driving factor contributing to the occurrence, spread and proliferation of antibiotic resistant bacteria (arb) and antibiotic resistance genes (args) in the environment. in this study, high-throughput sequencing based metagenomic approaches were employed to characterize the tempo-spacial changes of antibiotic resistome, bacterial community and their correlations in pig farming wastewater and its receiving ri ...	2017	28763642