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fine structure of babesia equi laveran, 1901 within lymphocytes and erythrocytes of horses: an in vivo and in vitro study.the development of babesia equi (piroplasmia) in the vertebrate host was studied by electron microscopy. the tick-borne sporozoites initiated an exoerythrocytic schizogony in lymphocytes in vivo and in vitro. in lymphocyte cultures the schizonts of b. equi lay as fissured bodies directly within the host cell forming numerous cytomeres. these cytomeres were bordered by a single membrane and contained polymorphous nuclei. merozoite differentiation was initiated at several places by protrusions app ...19836827432
periodic, multimodal distribution of granule volumes in mast cells.the areas of 2327 mast cell granules in transmission electron micrographs of sections of peritoneal mast cells from adult rats were measured by digitized planimetry. a histogram constructed using equivalent volumes calculated from the measured areas assuming approximation of the granules to spheres showed a periodic multimodal distribution in which the modes fell at volumes that were successively larger integral multiples of the volume at the first mode. application of a moving-bin technique to ...19836831525
cell membrane maturation of friend erythroleukemic cells and tocopherol-dependent erythropoietin effect: a scanning electron microscopic study.typical morphological features of surface structural alterations during friend cells differentiation are described. scanning electron microscopy (sem) revealed that dmso induction switched on cell alteration of the proerythroblast-like cells, possessing microvilli projections on cell membrane with some ruffles, to an advanced stage with a blebby surface. this was followed by the formation of a pear-like polarized cell separated into two zones by a narrow cytoplasmic bridge at the equatorial plan ...19826956453
morphology and laminar distribution of nonpyramidal neurons in the auditory cortex of the rabbit.a study of the morphology and laminar distribution of nonpyramidal neurons in golgi-nissl preparations of electrophysiologically verified auditory cortex was carried out in the adult rabbit. nonpyramidal neurons were located primarily within laminae i-iv and were only infrequently seen in lamina v and vi. in lamina i, four nonpyramidal cell types were observed: (1) small, spine-free horizontal neurons, (2) small, sparsely spined multipolar neurons with radiate dendrites, (3) large, multipolar ne ...19827119153
the role of industry in the development of a product for control of mycoplasmal plant diseases.a number of mycoplasmal or mycoplasma-like diseases of plants have been treated with an oxytetracycline-based product. remission of symptoms has generally resulted, and in some instances the local use of this product under temporary governmental registrations has been approved. the use of oxytetracycline for control of many such diseases is not commercially feasible because the potential market is relatively small and the costs of development are relatively high. however, oxytetracycline product ...19827123054
free sugars and sorbitol in fruits--a complication from the literature.the glucose, fructose, sucrose, and sorbitol content of apple, pear, plum, cherry, grape, strawberry, raspberry, blackberry, and peak fruit was compiled from the literature; their range, mean, standard deviation, and percent coefficient of variance were calculated. the individual fruits have characteristic patterns relating to their sorbitol content, glucose: fructose ratio, and sucrose content which are influenced to only a small degree by variety, season, or geographic origin. processing in ma ...19817204315
eimeriidae of the herbivorous mole-rat, spalax ehrenbergi nehring.a survey of 41 herbivorous mole-rats, spalax ehrenbergi nehring, in urfa, adiyaman, and maras provinces of turkey revealed 7 new species of eimeria in addition to previously described eimeriidae. the shape, average dimensions (in microgram) of their oocysts, and the numbers of hosts and from which the new species were isolated were as follows: eimeria urfensis sp. n., ellipsoidal (33 x 21), from 8 hosts; eimeria adiyamanensis sp. n., ovoid to ellipsoidal (33 x 18), from 6 hosts; eimeria haranica ...19807218183
vagally innervated and denervated gastric pouch in one and the same dog--a new model.a new model for studies of gastric secretion is introduced. two pouches separated from the stomach are constructed in one and the same dog. a vagally denervated pouch is taken from the corporo-fundic part of the stomach, and a vagally innervated pouch is constructed from a pear-shaped piece of the anterior wall with basis in the corporeal part of the lesser curvature. intact vagal innervation is tested with 2-deoxy-d-glucose. the maximal histamine response is used as reference value for each pou ...19807233067
ultrastructure of the pars intermedia of the adult sheep hypophysis.light microscopy of coronal sections of the sheep pars intermedia revealed a compact, incompletely lobulated v-shaped region about 15-20 cells thick, situated between the pars distalis and the pars nervosa. a prominent hypophysial cleft and follicles containing a colloid-like substance were seen. using electron microscopy, five cell types could be distinguished: pars intermedia glandular cells, pars distalis-like glandular cells, interstitial cells, follicular cells and cleft lining cells. the p ...19817249044
[spontaneous osteolysis of the carpus (gorham syndrome?)].osteolysis following trauma (vanishing bone) is well known. its appearance is not typical to any special anatomical site. a massive osteolysis of the carpus was described by gorham in 1955. in subsequent years this type of osteolysis was found together with progressive nephropathy. the author presents a case of exclusively carpal osteolysis without initial trauma, not accompanied by nephropathy, starting out at the age of six. the carpal bones were completely destroyed by the age of eleven.19807250806
[posttraumatic osteolysing haemangiomatosis (disappearing bone or gorham syndrome) (author's transl)].after injury to the palm of the left hand in a 24-year-old woman, haemangiomatosis - taking its origin from the site of urinary - rapidly spread over the left arm, with partially disappearance of metacarpals ii--v. previous angiodysplasia of the hand or enosseous haemangioma could be definitely ruled out. histological examination of the cavernous haemangioma gave no indication of malignancy. but the tumor spread to the axilla, without metastases. high-voltage radiation arrested the growth. seven ...19817285826
[radiotherapy of the gorham - stout syndrome (author's transl)]. 19817307181
radiotherapy in massive angiomatous osteolysis (gorham-stout's syndrome).the cases of two young men with the gorham-stout's syndrome of massive angiomatous osteolysis are presented. attempted surgical removal of the pathological angiomatous tissue proved unsuccessful. telegammatherapy with each patient receiving total focal dose of 40.0 gy fractionated over a period of 4 weeks resulted in speedy and complete arrest of osteolysis, and in partial recalcification of the bone tissue destroyed. in one patient the favourable condition has been lasting for 9 years, in the o ...19817330910
inhibition of protein synthesis in vitro by proteins from the seeds of momordica charantia (bitter pear melon).1. a haemagglutinating lectin was purified from the seeds of momordica charantia by affinity chromatography on sepharose 4b and on acid-treated sepharose 6b. it has mol.wt. 115 000 and consists of four subunits, of mol.wts. 30 500, 29 000, 28 500 and 27 000. 2. the lectin inhibits protein synthesis by a rabbit reticulocyte lysate with an id50 (concentration giving 50% inhibition) of approx. 5 micrograms/ml. protein synthesis by yoshida ascites cells is partially inhibited by the lectin at a conc ...19807378061
[cryosurgery of hemorrhoid disease].the kriosurgery of hemorrhoidal diseases represents a modern method of treating hemorrhoids, a therapeutic application of low temperatures on the alive human tissue. the critical temperature for destroying tissues in -20 degrees c. if temperature is above this freezing it won't a pear. the author chose a series of 70 patients with hemorrhoids to be treated by kriohemorrhoidoctomy. as a means of refrigerating nitrogenous oxide was used while the operative intervention was made at an outpatient cl ...19807405484
pear-shaped ('teardrop') configuration of the urinary bladder. excretory urography in a 63-year-old man. 19807411814
[radiodiagnosis of the gorham-stout syndrome (author's transl)]. 19807459960
gluco-oligosaccharide production by a strain of erwinia amylovora.when grown in sorbitol-asparagine-salts medium, cells of erwinia amylovora isolate t83 released a mixture of oligosaccharides and lipopolysaccharide in place of extracellular polysaccharide. the oligosaccharide fraction was usually readily separable from lipopolysaccharide by gel permeation chromatography, but some samples required disaggregation by preliminary treatment with edta. by 1h- and 13c-nmr spectroscopy, methylation analysis and mass spectrometry, the oligosaccharide fraction was shown ...19957476566
genetics of galactose metabolism of erwinia amylovora and its influence on polysaccharide synthesis and virulence of the fire blight pathogen.galactose metabolism mutants of erwinia amylovora were created by transposon insertions and characterized for their growth properties and interaction with plant tissue. the nucleotide sequence of the gale gene was determined. the gene, which encodes udp-galactose 4-epimerase, shows homology to the gale genes of escherichia coli, neisseria gonorrhoeae, rhizobium meliloti, and other gram-negative bacteria. cloned dna with the gale and with the galt and galk genes did not share borders, as judged b ...19947507102
analysis of the high molecular weight rhoptry complex of plasmodium falciparum using monoclonal antibodies.twenty-one monoclonal antibodies, obtained after immunization of mice with erythrocytic stages of plasmodium falciparum, produced a double dot image in ifa. immunoelectronmicroscopy indicated that the mabs reacted with the rhoptries. rhoptries are pear-shaped apical organelles, believed to be involved in invasion of the host cell by the parasite. the mabs all immunoprecipitated the high molecular weight antigen complex. some mabs recognized on immunoblots only 1 protein of this complex, whereas ...19947517517
synaptic connections of central carotid sinus afferents in the nucleus of the tractus solitarius of the rat. ii. connections with substance p-immunoreactive neurons.a combined transganglionic transport and immunocytochemical technique was used to study the synaptic morphology of central carotid sinus afferents and substance p-immunoreactive neurons in the commissural subnucleus of the nucleus of the tractus solitarius in rats. a large population of substance p-immunoreactive neurons (88.32%) were seen in close association with central carotid sinus afferents by light microscopy. however, many labelled central carotid sinus afferents appeared not associated ...19947522269
investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by pcr and competitive inhibition enzyme-linked immunosorbent assay.development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (sa-mcf) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. a recently developed competitive-inhibition enzyme-linked immunosorbent assay (ci-elisa) for the detection of antibody to malignant catarrhal fever (mcf) virus (mcfv) in ruminants based on a monoclonal antibody to a widely conserved epitope ...19957559946
pear blister canker viroid: sequence variability and causal role in pear blister canker disease.the sequences of several cdna clones of pear blister canker viroid (pbcvd) p1914t and p47a isolates have been determined. seven out of eight p1914t clones analysed have a constant sequence which differs at six positions from that of the p2098t isolate reported previously. the remaining p1914t clone (8) has a single nucleotide substitution. the same six changes have been also observed in most of the ten p47a clones sequenced. however, some p47a clones show additional variability in positions on b ...19957595369
genetic analysis of nonpathogenic agrobacterium tumefaciens mutants arising in crown gall tumors.little is known about the effect of the host on the genetic stability of bacterial plant pathogens. crown gall, a plant disease caused by agrobacterium tumefaciens, may represent a useful model to study this effect. indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria suggest that the host plant might induce loss of pathogenicity in populations of a. tumefaciens. here we report that five different a. tumefaciens strains initially isolated from appl ...19957601840
nucleotide-dependent angular change in kinesin motor domain bound to tubulin.kinesin is a 'motor' molecule, consisting of two head domains, an alpha-helical coiled coil rod, and a tail part that binds to its cargo. when expressed in a bacterial system, the head domain is functional, and can bind to microtubules with the stoichiometry of one head per tubulin dimer. kinesin moves along microtubules by means of a cyclic process of nucleotide binding, hydrolysis and product release. we have used negative-stain electron microscopy and image analysis to study the structures of ...19957617042
the control of polyphenol oxidase activity in fruits and vegetables. a study of the interactions between the chemical compounds used and heat treatment.objective of this research was to find alternative methods for the control of polyphenol oxidase (ppo) activity in fruits and vegetables with the purpose of reducing or eliminating the use of so2 for this purpose. interactions between the use of ascorbic acid, citric acid, edta, sodium metabisulphite and heat treatment (70 degrees c for 2 min) in the control of ppo activity were studied in avocado (var. fortuna), banana (var. nanica), apple (var. ana, fuji, gala & golden), pear (var. d'agua), pe ...19957659702
massive osteolysis of the pelvis, femur and sacral bone with a gorham-stout syndrome.we report a case of a 49-year-old white woman with gorham-stout syndrome. this very rare massive osteolysis occurred in the left pelvis and included the femur and the sacral bone. histology study revealed typical ectatic vessels covered with endothelium. the therapy of choice in this region is radiotherapy with a dose of 40 gy. however, in our case this therapy only led to short-term pain relief and cessation of the osteolysis. stabilization with corticospongious bone did not seem to be effectiv ...19957662475
molecular cloning of cdnas encoding the protein backbones of arabinogalactan-proteins from the filtrate of suspension-cultured cells of pyrus communis and nicotiana alata.this paper reports the isolation of cdnas encoding the protein backbone of two arabinogalactan-proteins (agps), one from pear cell suspension cultures (agppc2) and the other from suspension cultures of nicotiana alata (agpna2). the proteins encoded by these cdnas are quite different from the 'classical' agp backbones described previously for agps isolated from pear suspension cultures and extracts of n. alata styles. the cdna for agppc2 encodes a 294 amino acid protein, of which a relatively sho ...19957670506
concurrent validity of the cognitive component of schizophrenia: relationship of panss scores to neuropsychological assessments.cognitive symptoms of schizophrenia may represent a separate component of the disorder that is distinct from positive or negative syndromes. in a previous study, we reported a factor analysis of the positive and negative syndrome scale (panss) that revealed five components, one of which we labeled the cognitive component. in the present study, we explored the validity of the panss cognitive component by examining correlations between neuropsychological measures and the five factor-analytically d ...19947701028
[gorham-stout massive ostelysis. a case report].one case of massive osteolysis gorham-stout is presented. idiopathic osteolysis is a rare disease. the coincidental radiological diagnosis following minor trauma or a few months' history of mild pain involving joints is characteristic. the disease process has a sudden onset. bones undergo partial or complete resorption, which may spread across joints to contiguous bones. the osteolytic process eventually ceases spontaneously, but generally not before severe deformation and destruction of bone ha ...19957709224
cognitive deficits in schizophrenia. prediction of symptom change for participators in work rehabilitation.while recent studies have found that most patients with schizophrenia who participate in work rehabilitation experience symptom improvement, there appears to be a subgroup of patients whose symptoms remain stable or even worsen. the present study explores the hypothesis that cognitive impairment is one factor which reduces the clinical effectiveness of rehabilitation and accounts for a lack of improvement in some patients. to explore this question, performance on two neuropsychological tests was ...19957745389
identification of allergens in fruits and vegetables: ige cross-reactivities with the important birch pollen allergens bet v 1 and bet v 2 (birch profilin).in this study serum samples collected from 20 patients with birch pollen allergy were investigated. all patients had experienced allergic symptoms after contact with or ingestion of particular fresh fruits and vegetables known as birch pollen-related foods.19957751517
production of patulin by different strains of penicillium expansum in pear and apple cultivars stored at different temperatures and modified atmospheres.it was shown that the ability of three different strains of penicillium expansum (nrrl 2034, nrrl 6069, and cbs 481.84) to grow and to produce patulin in pears (cv. spadona) and apples (cv. starking) varied under the different temperatures tested (0, 3, 6, 17 and 25 degrees c). strain nrrl 2034 did not produce patulin at 0 or 25 degrees c while the two other strains produced the toxin at all temperatures, the maximum production occurring at 25 degrees c (for pears) and 17 degrees c (for apples). ...19957758631
human eosinophil peroxidase enhances tumor necrosis factor and hydrogen peroxide release by human monocyte-derived macrophages.inhibition of growth or eradication of experimentally induced tumors has been shown to be accompanied by infiltration of eosinophils and macrophages into the tumor mass. since macrophages are important mediators of host antitumor activity, the possibility arises that a collaboration may exist between these two cell types in the control of tumor growth. in this study, we report the effect of eosinophil peroxidase (epo), a basic protein contained in eosinophils that binds to several cell types inc ...19957774640
the indian laburnum (cassia fistula l.) fruit: an analysis of its chemical constituents.the edible fruit tissue of indian laburnum fruit (cassia fistula l.), a member of the leguminosae family, was analyzed for certain organic compounds and mineral nutrients. of the nine macro- and micronutrients studied, k was the most highly concentrated such that 100% of the us recommended dietary allowances (rda) for adults could be met by the consumption of about 100 g of the fresh fruit. na contents in pulp and seeds are relatively low. ca content at 827 mg per 100 g of dry matter is one of t ...19957784398
[idiopathic massive osteolysis of the femur. syndrome called gorham-stout syndrome].a case of massive osteolysis (disappearing bone disease, phantom bone, gorham's massive osteolysis) was observed in a young african man with two lesions of the femur. this is a very rare syndrome characterized by an acute spontaneous resorption of bone, without any sign of malignant or infectious disorder. the lesion may develop in any part of the skeleton, with extensive bone loss, but is benign. bone is replaced by haemangiomatous tissue. the diagnosis lead on the association of clinical, radi ...19957784404
gorham disease of the spine.this is a case report of gorham disease of the spine with review of the literature.19947809757
genetic and plasmid diversity within natural populations of pseudomonas syringae with various exposures to copper and streptomycin bactericides.we examined the genetic and plasmid diversity within natural populations of pseudomonas syringae isolated from three ornamental pear nurseries in eastern oklahoma. the bactericide spray regimen differed at each nursery; copper and streptomycin, only copper, and no bactericides were applied at nurseries i, ii, and iii respectively. resistance to copper (cur) and resistance to streptomycin (smr) were determined for 1,938 isolates of p. syringae; isolates from nurseries i and ii were generally cur ...19947811082
sexual dimorphism and developmental change of the salivary glands in adult culicoides variipennis (diptera: ceratopogonidae).salivary glands of adult male and female culicoides variipennis (coquillett) were sexually dimorphic when examined by phase contrast light microscopy. female salivary glands were larger and more complex than those in males. each female gland consisted of a main gland, which was subdivided into a proximal neck and a distal body with reference to the salivary duct, and four accessory glands. each male salivary gland consisted of a pear-shaped body with a constriction, or neck, that divided it into ...19947815404
allergy to latex, avocado pear, and banana: evidence for a 30 kd antigen in immunoblotting.allergens of natural latex, latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated latex and fruit allergies. extracts were separated by sodium dodecyl-sulfate-polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. after incubation with patients' sera, ige antibodies were revealed by a goat anti-human ige alkaline-phosphatase conjugate. seventeen serum samples from patients with well-documen ...19957852672
[validity of the brief psychiatric rating scale].the internal structure and validity of terms used in the brief psychiatric rating scale (overall and gorham, 1962) was analyzed. the data which had been gained at the entrance examination of 1557 inpatients with the diagnosis according to icd-9 (schizophrenia simplex, schizophrenia paranoids, schizoaffective disease depressive type, schizoaffective disease manic type, manic-depressive disease manic phase, manic-depressive disease depressive phase) were processed using stepwise discriminant analy ...19947882071
maturation of somatostatin immunoreactivity in the pigeon retina: morphological characterization and quantitative analysis.in addition to a modulatory function, somatostatin (ss) is likely to exert a morphogenetic and/or trophic role in the developing nervous system. in this study, a mouse monoclonal antibody directed to ss was used to investigate the posthatching development of ss-immunoreactivity (ss-ir) in the pigeon retina to provide a basis for a better understanding of the role of this peptide in retinal maturation. in the adult, ss-ir was observed in amacrine cells located in the inner nuclear layer (inl) of ...19947912104
immunohistochemical localization of neuropeptide y and somatostatin in human fetal retina.the localization of neuropeptide y and somatostatin in the retina of six prenatal human specimens was determined by the utilization of light microscopic immunoperoxidase and immunofluorescence methods. the age of the prenatal fetuses ranged from 15 to 40 weeks of gestation. at 15 weeks, round or pear-shaped neuropeptide y-immunopositive cells were observed in the inner nuclear layer and somatostatin immunoreactivity was only detected in the ganglion cell layer. positive neuropeptide y ganglion c ...19947936198
molecular cloning of a gene encoding an arabinogalactan-protein from pear (pyrus communis) cell suspension culture.arabinogalactan-proteins (agps) are proteoglycans containing a high proportion of carbohydrate (typically > 90%) linked to a protein backbone rich in hydroxyproline (hyp), ala, ser, and thr. they are widely distributed in plants and may play a role in development. the structure of the carbohydrate of some agps is known in detail but information regarding the protein backbone is restricted to a few peptide sequences. here we report isolation and partial amino acid sequencing of the protein backbo ...19947937945
falciparum malaria in naturally infected human patients: viii. fine structure of intraerythrocytic asexual forms before and during chloroquine treatment.the fine structure of the intraerythrocytic asexual forms of plasmodium falciparum is described before and during treatment with chloroquine (cq) in the venous blood of 27 saudi patients whe were heavily infected with the parasite. before treatment, the "pear" shaped merozoite was rapidly transformed into a ring-shaped trophozoite. this was enclosed in a parasitophorous vacuole bounded by a single membrane derived from the host erythrocyte. the parasite feeding mechanism involves several previou ...19947951397
ct and mri of massive osteolysis of gorham.massive osteolysis of gorham is a clinical, radiographic, and histologic entity characterized by histologically benign vascular proliferation involving bone associated with extensive osteolysis. we present ct and mri findings of the disease and serial radiography following the progressing bone destruction.20167962814
ct and mri of gorham syndrome.gorham syndrome, also known as massive osteolysis or vanishing bone, is a rare disorder (135 cases reported) leading to extensive loss of bony matrix, replaced by proliferating thin-walled vascular channels. three histologically proven cases of the disease are reported, including the clinical presentation and modern imaging features with ct (with 3d reconstruction) as well as t1- and t2-weighted mri. two cases in young women were located in the pelvis with extensive osteolysis reaching to the ac ...20167962815
a three-dimensional reconstruction study of the rough er-golgi interface in serial thin sections of the pancreatic acinar cell of the rat.distinctive views of the tubulo-vesicular elements interposed between the endoplasmic reticulum (er) and the golgi apparatus were obtained in thin sections. the tubules that protrude from the transitional rough er (trer) are of dissimilar length. the numbers of tubules and of the nearby omega- and pear-shaped profiles decrease after fasting and are partially restored by refeeding. this formation is designated herein as the budding chamber of the trer. close to the budding chamber, clusters of 56 ...19948006070
[inflammatory gorham disease].the case of a 48-year old female with massive osteolysis since the age of 13 years is reported. she had inflammatory flares which resolved spontaneously or after a transient discharge of lymph.19938012338
nucleotide sequences of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex- and carlaviruses.the nucleotide sequence (9306 nucleotides) of cdna clones of apple stem pitting virus (aspv) obtained from a double-stranded rna template, extracted from diseased plant tissue, was determined. the genome is composed of five open reading frames (orfs) encoding putative proteins with m(r)s of 247083, 25147, 12832, 7429 and 43712, and has a poly(a) tail. using two oligonucleotides designed from the aspv sequence information a 1598 bp fragment from near the 3' terminus of the viral rna, containing t ...19948021584
clinical features of a previously undescribed codon 216 (proline to serine) mutation in the peripherin/retinal degeneration slow gene in autosomal dominant retinitis pigmentosa.mutations in the human peripherin/retinal degeneration slow (rds) gene have been found in patients with macular dystrophies as well as in those with autosomal dominant retinitis pigmentosa. the authors studied the clinical features in members of two families with autosomal dominant retinitis pigmentosa and a previously unreported mutation in the peripherin/rds gene.19948058286
structure and expression of an inhibitor of fungal polygalacturonases from tomato.a polygalacturonase inhibitor protein (pgip) was characterized from tomato fruit. differential glycosylation of a single polypeptide accounted for heterogeneity in concanavalin a binding and in molecular mass. tomato pgip had a native molecular mass of 35 to 41 kda, a native isoelectric point of 9.0, and a chemically deglycosylated molecular mass of 34 kda, suggesting shared structural similarities with pear fruit pgip. when purified pgips from pear and tomato were compared, tomato pgip was appr ...19948061315
disturbance of alveolar lining layer: effects on alveolar microstructure.to further study the influence of altered surface tensions on alveolar micromechanics, we analyzed the structure-function relationships in excised rabbit lungs filled with or rinsed by a fluorocarbon (approximately 15 mn/m) or by hexadecane (approximately 25 mn/m). the lungs were fixed and dehydrated by vascular perfusion, and the tissue samples were analyzed by light, transmission, and scanning electron microscopy. we made three observations. 1) pressure-volume (p-v) loops hexadecane-filled lun ...19948063660
molecular characterization of a polygalacturonase inhibitor from pyrus communis l. cv bartlett.a polygalacturonase inhibitor glycoprotein with an apparent molecular mass of 43 kd was purified from pear (pyrus communis l. cv bartlett) fruit. chemical deglycosylation of this protein decreased the molecular mass to 34 kd. gas chromatographic analysis suggests that n-linked glycosylation accounts for the majority of sugar moieties. partial amino acid sequence analysis of the purified polygalacturonase inhibitor protein provided information used to amplify a corresponding cdna by polymerase ch ...19938108494
aberrant histoplasma capsulatum. confirmation of identity by a chemiluminescence-labeled dna probe.a cottony, light tan, filamentous fungus with pear-shaped microconidia and lacking tuberculated macroconidia was isolated from a bronchial lavage specimen. subculture on several media at 37 degrees c failed to convert the fungus to a yeast form after several weeks; attempts at in vivo conversion in mice were also unsuccessful. sera obtained several months apart showed m bands with histoplasma capsulatum (hc) antigen by immunodiffusion and an increase in complement fixation titers with mycelial a ...19938112034
the transcription factor bsap (nf-hb) is essential for immunoglobulin germ-line epsilon transcription.treatment of murine splenic b lymphocytes and certain b-lineage cell lines with mitogen (lps) and the lymphokine il-4 has been shown to induce expression of germ-line epsilon transcripts (l epsilon transcripts) and class switching to the c epsilon gene. three protein complexes, one of which (complex 3) is constitutively expressed, have been shown to bind to a 179-base pair lps/il-4-responsive l epsilon promoter (rothman, p., s. c. li, b. gorham, l. glimcher, f. w. alt, and m. boothby. 1991. mol. ...19948144891
hair cells of different shapes and their placement along the frog crista ampullaris.the list of distinguishing morphological features of hair cells includes: type i and type ii afferent innervation, and length, shapes and arrangements of stereo- and kinocilia. we now add to this list the shapes of the hair cells themselves and their placement within the mechanosensory organ, in this case the semicircular canal. although hair cells of the crista ampullaris of the frog are only of type ii they may now be further classified into three sub-groups according to shape: club-, cigar- a ...19948157499
cytoplasmic forms of fission yeast casein kinase-1 associate primarily with the particulate fraction of the cell.two novel casein kinase-1 homologs, cki1+ and cki2+, have been isolated from schizosaccharomyces pombe and characterized. both genes reside on chromosome ii and encode approximately 50-kda proteins that are related structurally and enzymatically to the yck gene products of budding yeast. subcellular fractionation experiments demonstrate that cki1 and cki2 are both cytoplasmic enzymes that do not overlap in subcellular distribution and that probably play distinct roles within the cell. although g ...19948163505
muscle- and pneumatic-powered counterpulsating lvads: a pilot study.there is a worldwide interest in supporting the failing heart with a skeletal muscle by either wrapping it around the natural heart (dynamic cardiomyoplasty) or by constructing a skeletal muscle ventricle (smv) used for counterpulsation. conventional cardiomyoplasty in many clinics carries an operative mortality rate of 15-20% partly because it requires 6 weeks to train the muscle to contract continually. a flexible, pear-shaped blood pump with an inflatable air chamber was designed and made aro ...19948185491
isolation and characterization of rhamnogalacturonan oligomers, liberated during degradation of pectic hairy regions by rhamnogalacturonase.digests of modified hairy regions of apple pectin (mhr) obtained after degradation by rhamnogalacturonase (rgase) were analyzed for oligomer composition using high-performance anion-exchange chromatography and pulsed amperometric detection. a series of oligomers which appear to be characteristic of rgase degradation could be recognized. these oligomers were isolated on a preparative scale by size-exclusion chromatography and preparative anion-exchange chromatography and analyzed for sugar compos ...19948194077
prickly pear (opuntia sp.) pectin alters hepatic cholesterol metabolism without affecting cholesterol absorption in guinea pigs fed a hypercholesterolemic diet.prickly pear pectin intake decreases plasma ldl concentrations by increasing hepatic apolipoprotein b/e receptor expression in guinea pigs fed a hypercholesterolemic diet. to investigate whether prickly pear pectin has an effect on cholesterol absorption and on enzymes responsible for hepatic cholesterol homeostasis, guinea pigs were fed one of three semipurified diets, each containing 15 g lard/100 g diet: 1) the lard-basal diet with no added cholesterol or prickly pear pectin (lb diet); 2) the ...19948207539
case report 803: gorham's disease (gorham-stout syndrome) of scapula.in summary, a 36-year-old man presented with pain and limited motion in the shoulder. clinical examination revealed obliteration of normal scapular landmarks in the conspicuous absence of any palpable soft tissue mass. roentgenograms showed progressive osteolysis of the scapula. biopsy confirmed the diagnosis of gorham's disease. mri played a key role in defining the extent of disease involvement and in displaying the distinct soft tissue anatomy. these mri features are to our knowledge previous ...19938248825
intragenic sequences are required for cell type-specific and injury-induced expression of the rat peripherin gene.peripherin is a 57 kda type iii intermediate-filament protein that is thought to play a role in axonogenesis both during development and following nerve injury (oblinger et al., 1989; escurat et al., 1990; gorham et al., 1990; troy et al., 1990b). we have used transgenic mouse technology to define peripherin gene sequences that are necessary for cell type-specific expression and for the increase in peripherin that occurs in response to axonal injury. correct temporal and nervous system-specific ...19938254361
kasabach-merritt syndrome with bone involvement: the pseudomalignant sign of gorham. 19938315070
[reactivity of chlamydia pneumoniae strains in the ideia chlamydia test kit designed for detection of chlamydia trachomatis].the intensity of reaction in the ideia chlamydia test kit to lipopolysaccharide of seven strains of chlamydia pneumoniae was examined by using highly purified elementary bodies (ebs). the strains were divided into two groups according to whether the ebs were pear-shape or round. the group with the pear-shaped ebs consisted of tw-183, ar-39 and ar-388 strains; the group with the round ebs consisted of iol-207, kajaani-6, yk-41 and kkpn-1 strains. the number of ebs at the cutoff level of the test ...19938336010
[dendritic arborization patterns of interneurons labeled with a lectin, vicia villosa, in rat cerebral cortex: studies by intracellular injection of lucifer yellow using aldehyde-fixed slices].in order to characterize the dendritic field of a number of interneurons in the cerebral cortex, the labeling of extracellular sugar chains which define a subset of interneurons was combined with the subsequent intracellular filling of dyes in aldehyde-fixed tissue. neurons whose cell body had been outlined by a lectin, vicia villosa (vva), which recognizes terminal n-acetylgalactosamine, were intracellularly injected with a fluorescent tracer, lucifer yellow (ly), in the rat parietal cortex und ...19938337931
life cycle of leishmania major (kinetoplastida: trypanosomatidae) in the neotropical sand fly lutzomyia longipalpis (diptera: psychodidae).the development of leishmania major yakimoff & schokhor in the new world sand fly lutzomyia longipalpis (lutz & neiva) was examined by light and electron microscopy. in this unnatural host, parasites differentiated into 10 typical morphological forms, multiplied at three sites, migrated anteriorly and established in the foregut, and attached to gut surfaces. in the blood meal, amastigotes divided and transformed into two successive dividing, stumpy promastigote stages. elongate nectomonad promas ...19938360894
sensory receptor-like cells in the human foetal vomeronasal organ.the vomeronasal organ (vno) was studied in ten human foetuses, 12 to 36 weeks old. at 12 to 23 weeks, it was lined by a smooth pseudostratified epithelium, with neurone-specific enolase (nse) positive cells looking like olfactory receptors. clusters of nse-positive cells were seen in relation with the posterosuperior end of the organ and along nerve fascicles in the nasal septum. at 36 weeks, the organ was lined by a respiratory epithelium and did not show any receptor-like cells; some pear-shap ...19938369475
isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis.we isolated a fission yeast putative protein serine/threonine phosphatase gene designated ppe1+ by hybridization. the predicted amino acid sequence is similar to those of the fission yeast ppa2 (53% identity) and dis2 (39%) phosphatases, and highly similar to those of the budding yeast sit4 (72%), drosophila ppv (68%) and rabbit ppx (61%) phosphatases. antibodies against ppe1 protein identified a 37-kd polypeptide in fission yeast. a gene disruption (designated delta ppe1) caused cold-sensitive ...19938387356
isolated brush cells of the rat stomach retain their structural polarity.the brush cells (bc) are highly polarized elements occurring in epithelia of endodermal origin. they have a preferential topographical distribution in the organs in which they reside. in the stomach of the rat, bc prevail near the transitional zone separating the forestomach from the glandular stomach. thus, a method was developed to isolate and recover bc from this organ with the aim of investigating the changes they may undergo after dissociation. strips of the rat stomach were severed from th ...19938443835
demonstration of chlamydia pneumoniae in atherosclerotic lesions of coronary arteries.chlamydia pneumoniae is a human respiratory pathogen that causes acute respiratory disease and approximately 10% of community-acquired pneumonia. the infections are geographically widespread. antibody prevalence studies have shown that virtually everyone is infected with the c. pneumoniae organisms at some time and that reinfection is common. in addition to respiratory disease, seroepidemiologic studies have shown an association of this organism with coronary artery disease. c. pneumoniae was de ...19938450249
fertility awareness as a component of sexuality education. preliminary research findings with adolescents.fertility awareness refers to the observation and interpretation of cervical mucus, often called vaginal discharge. a woman's cervical-mucus pattern indicates the time of ovulation and differentiates the fertile and infertile phases of the menstrual cycle from each other. fertility awareness enables a woman to know when pregnancy can and cannot occur on a daily basis during each menstrual cycle. there has been, to date, almost no exploration of the appropriateness of fertility-awareness instruct ...19938459941
leishmania differentiation in natural and unnatural sand fly hosts.leishmania differentiation in the gut of phlebotomine sand flies was evaluated based on five light and electron microscopic studies of natural (leishmania panamensis/lutzomyia gomezi, leishmania chagasi/lutzomyia longipalpis) and unnatural (leishmania mexicana/lutzomyia abonnenci, leishmania panamensis/phlebotomus papatasi, leishmania major/lutzomyia longipalpis) life cycles. in the bloodmeal, transformation of amastigotes into stumpy promastigotes occurred before or during division. further div ...19938461893
[clinico-pathological and orthopedic-surgical aspects of essential osteolysis (gorham disease)].authors present two cases. one of their patient was followed for 30 years. they discuss the pathological characters and diagnostic difficulties of the disease. they have observed that with radical operations of--according to necessity--serial surgical interventions the outcome of patients can be properly controlled against recent pessimistic opinions.19938464630
pregnancy complicated by gorham disease.a pregnancy with gorham disease (massive osteolysis syndrome) is described. to our knowledge, this is the first reported case of a pregnancy associated with this disease.19938469480
fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro.culturing of rabbit pre-implantation embryos was performed in ham's f10 medium supplemented with polyvinylpyrrolidone. under these culture conditions, day 6 post coitum blastocysts increased their diameter within 24 h to 80% of that of day 7 blastocysts grown in vivo. despite this substained growth, the embryonic disc remained undifferentiated with clear signs of degeneration after 24 h of culture. basic fibroblast growth factor (bfgf) was able to overcome this developmental block. after 12 h of ...19938470827
mycoplasmalike organisms (mlos) and distinguish them from other mlos.dna of 10 lines of rice yellow dwarf (ryd) mycoplasmalike organisms (mlos) from japan, the phillippines, and thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal dna of a tochigi (japan) line of ryd mlo. one chromosomal probe (ryd9) and all six extrachromosomal probes hybridized with various other mlos (sugarcane white leaf, onion yellows, cineraria witches'-broom, japanese hornwort witches'-broom, water dropwort wiches'-broom, gentian witches'-b ...19938489230
two novel protein kinase c-related genes of fission yeast are essential for cell viability and implicated in cell shape control.two novel protein kinase c (pkc)-like genes, pck1+ and pck2+ were isolated from fission yeast by pcr. both contain common domains of pkc-related molecules, but lack a putative ca(2+)-binding domain so that they may belong to the npkc group. gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability. cells of a single pck2 deletion display severe defects in cell shape; they are irregular and sometimes pear-like instead of cylindrical. in co ...19938491190
the morphology of chlamydia pneumoniae.the morphology of a recently isolated strain of chlamydia pneumoniae, yk-41, was compared by electronmicroscopy with c. pneumoniae twar, chlamydia trachomatis l2/434/bu and chlamydia psittaci cal 10. the results showed that "pear-shaped" morphology was not typical of c. pneumoniae. basic morphological features, such as surface projections and hexagonally arrayed, regular structures in the inside layer of the outer membrane of elementary bodies, were very similar in these strains. the structure o ...19938510134
context effect of common objects on visual processing.in previous experiments on visual processing it was shown that correct identification of sequentially and simultaneously presented numerals declined as the presentation interval was decreased from 50.0 to 16.7 ms/numeral. to determine how performance was influenced in context, common everyday objects were used in place of the numerals. these objects were grouped into 36 different categories with 4 objects per category. an example of a category was fruit: apple, banana, pear, pineapple. for all e ...19958539008
promotion of gastrulation by maternal growth factor in cultured rabbit blastocysts.rabbit blastocysts of day 6 post coitus were cultured in a chemically defined, protein-free medium for 24 h. although the trophoblast continued to grow, the embryonic disc degenerated. addition of basic fibroblast growth factor (fgf-2, of human recombinant or bovine origin, 10 ng/ml) to the culture medium resulted in significant developmental progress. the embryonic disc became pear-shaped showing a round anterior edge and a posterior node. the primitive streak and hensen's node indicated that g ...19958581917
meat allergy: i--specific ige to bsa and osa in atopic, beef sensitive children.the use of lamb meat products has been suggested as an alternative diet for polyallergic children, although until now this clinical practice has not been supported by in-depth biochemical/immunological studies. the aims of this research were: to evaluate cross-reactivity between lamb and beef; to evaluate the role of bsa and osa as allergens in beef allergic children; and to evaluate cross-reactivity between bsa and osa.19958586772
interleukin-6: a potential mediator of the massive osteolysis in patients with gorham-stout disease.gorham-stout disease (gsd) or massive osteolysis, is an extremely rare osteolytic condition that involves extensive locally aggressive resorption of bone. the etiology and pathophysiology are unknown, and the role of the osteoclast in gsd is unclear. we studied a patient with gsd who had massive resorption of his mandible, which extended to his maxilla, zygoma, right parietal region, and cranium. to investigate the cause of the extensive resorption, we tested the effects of the patient's serum, ...19968626854
self-incompatibility (s) alleles of the rosaceae encode members of a distinct class of the t2/s ribonuclease superfamily.stylar ribonucleases (rnases) are associated with gametophytic self-incompatibility in two plant families, the solanaceae and the rosaceae. the self-incompatibility-associated rnases (s-rnases) of both the solanaceae and the rosaceae were recently reported to belong to the t2 rnase gene family, based on the presence of two well-conserved sequence motifs. here, the cloning and characterization of s-rnase genes from two species of rosaceae, apple (malus x domestica) and japanese pear (pyrus seroti ...19968676858
phytoplasma-specific pcr primers based on sequences of the 16s-23s rrna spacer region.in order to develop a diagnostic tool to identify phytoplasmas and classify them according to their phylogenetic group, we took advantage of the sequence diversity of the 16s-23s intergenic spacer regions (srs) of phytoplasmas. ten pcr primers were developed from the sr sequences and were shown to amplify in a group-specific fashion. for some groups of phytoplasmas, such as elm yellows, ash yellows, and pear decline, the sr primer was paired with a specific primer from within the 16s rrna gene. ...19968702291
genetic transfer of amylovoran and stewartan synthesis between erwinia amylovora and erwinia stewartii.dna fragments with ams genes of erwinia amylovora and cps genes of erwinia stewartii were transferred to exopolysaccharide (eps)-deficient mutants of the other species. the resulting epss were characterized by sensitivity to eps-dependent bacteriophages, staining with amylovoran-specific fluorescein-isothiocyanate-labelled lectin and chemical techniques, such as determination of the sugar composition and methylation analysis in order to distinguish between amylovoran and stewartan. degradation b ...19968704950
morphological indications of hair cell neodifferentiation in the organ of corti of amikacin treated rat pups.postnatal rats were treated with amikacin during the period of cochlear supra-normal sensitivity to ototoxic antibiotics, i.e. from day 9 to day 16 when the organ of corti is achieving its maturation. scanning and transmission electron microscopies were used to evaluate morphological changes in the organs of corti at different post-treatment periods, up until postnatal day 90. a massive loss in outer (ohcs) and inner (ihcs) hair cells was observed from as early as day 17. a prominent feature in ...19968762976
determination of avermectin b1 in biological samples by immunoaffinity column cleanup and liquid chromatography with uv detection.a simple and specific procedure for determination of avermectin b1 in biological samples is described. a specific anti-avermectin b1 immunosorbent was developed. avermectin b1 was extracted from cattle plasma, cattle meat, and pear samples. the extracts were cleaned up on a solid-phase extraction column (c18) and an immunoaffinity column. avermectin b1 was determined by reversed-phase liquid chromatography with uv detection at 245 nm. recoveries of avermectin b1 from samples spiked at levels of ...19968823915
left intraventricular balloon pump optimization during intractable cardiac arrest.this work aims to determine optimal balloon shape and volume during left intraventricular balloon pumping (iabp) in the fibrillating dog heart. a balloon volume equal to the left ventricular end-diastolic volume (lvedv) maintained a higher systolic aortic pressure and flow (106.4 +/- 2.7 mmhg and 84.7 +/- 2.35 ml/kg/min, x +/- sem, respectively) than a 25% smaller (97.8 +/- 3.3 mmhg, p = 0.002 and 63.7 +/- 4.1 ml/kg/min, p = 0.002, respectively) or a 25% larger balloon (87.4 +/- 2.3 mmhg, p = 0. ...19968841857
gorhams disease: a literature review and case reports. 20048872763
identification and partial amino acid sequences of seven s-rnases associated with self-incompatibility of japanese pear, pyrus pyrifolia nakai.s-allele-specific proteins (s-proteins) were separated and identified by two-dimensional (2d) gel electrophoresis from the style extract of 14 cultivars of japanese pear, pyrus pyrifolia nakai, which exhibits gametophytic self-incompatibility. these s-proteins were 30-32 kda basic proteins with putative pis of 9.6-10.1 and were distinct from the other proteins, which were common for all cultivars examined. each s-protein was assigned to a given s-genotype based on electrophoretic mobility and th ...19968889818
aflatoxin and cyclopiazonic acid production by a sclerotium-producing aspergillus tamarii strain.the production of aflatoxins b1 and b2 by aspergillus tamarii (subgenus circumdati section flavi) is reported for the first time. the fungus was isolated from soil collected from a tea (camellia sinensis) field in miyazaki prefecture, japan. three single-spore cultures, nrrl 25517, nrrl 25518, and nrrl 25519, were derived from subcultures of the original isolate 19 (mz2). each of these single-spore cultures of a. tamarii produced aflatoxins b1 and b2 and cyclopiazonic acid, as well as black, pea ...19968899995
massive osteolysis (gorham-stout syndrome) in the maxillofacial region: an unusual manifestation.an unusual case of massive osteolysis affecting the left side of the mandible, the maxilla, the zygoma, and the orbit is reported. the disease extended over a period of 25 years. the special feature of this case is that there were, before the osteolysis appeared, enlargement and sclerosis of the maxillofacial bones reaching up to the middle cranial fossa. histopathologic examination of the affected mandible revealed that the bone was replaced by fibrous tissue.19968961021
ige binding to a new cross-reactive structure: a 35 kda protein in birch pollen, exotic fruit and other plant foods.food allergies in birch pollen allergic patients have been shown to be due to cross-reactivities of specific ige antibodies which are directed against birch pollen allergens with related proteins in fruit, nuts and vegetables. we identified a new cross-reactive structure of 35 kda in birch pollen and some plant food extracts by enzyme allergosorbent test (east) and immunoblot inhibition studies. the 35 kda birch pollen protein is a minor allergen to which approximately 10-15% of birch pollen all ...19969000332
enumeration of bacterial and yeast colonists of apple fruits and identification of epiphytic yeasts on pear fruits in the pacific northwest united states.a procedure for the isolation of diverse culturable microflora for the estimation of the population size of yeasts and bacteria on the surface of pome fruits is described. maximum numbers of morphologically distinct colonies of both yeasts and bacteria were recovered from apple fruit surfaces when fruits were shaken for 5 min in sterile phosphate buffer plus tween, sonicated for 5 min, and aliquots of the buffer plated onto diluted yeast malt agar and diluted nutrient broth agar, respectively. t ...19969022303
location of cysteine and cystine residues in s-ribonucleases associated with gametophytic self-incompatibility.s-ribonucleases (s-rnases) that cosegregate with s-alleles in the styles of solanaceous and rosaceous plants are associated with gametophytic self-incompatibility (gsi). the amino acid sequences of many s-rnases have been derived from cdna sequences, but the state of half-cystines has not been clarified. we report the locations of the two free cysteine residues and four disulfide bridges of tobacco s6-rnase and of the four disulfide bridge of japanese pear s4-rnase. the protein was first s-pyrid ...19969022690
a simple and rapid method for isolation of high quality genomic dna from fruit trees and conifers using pvp.because dna degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality dna from plants containing a high content of polyphenolics has been a difficult problem. we demonstrate an easy extraction process by modifying several existing ones. using this process we have found it possible to isolate dnas from four fruit trees, grape (vitis spp.), apple (malus spp.), pear (pyrus spp.) and persimmon (diospyros spp.) and four species of conifer, pinus de ...19979023124
gorham syndrome of the thorax and cervical spine: ct and mri findings.gorham syndrome is a rare disorder that is characterized by local osseous invasion and surrounding soft tissues by an angiomatous mass, eventually causing lysis of the affected bone. to date, only four cases have reported the mr imaging appearance of this disease and the findings have been variable. we present a case involving the cervical and thoracic spine and part of the osseous hemithorax with attention to the mr findings.19979040145
the prevalence of latex allergy in children seen in a university hospital allergy clinic.natural rubber latex allergy is responsible for a wide spectrum of clinical symptoms, ranging from rhinoconjunctivitis to severe anaphylaxis, in both adults and children. an association between allergy to latex and allergy to various fruits has been reported. this study investigated the prevalence and clinical significance of latex sensitization in children seen in a university hospital allergy clinic. a total of 453 consecutive children were screened in a 7-month period. a detailed clinical his ...19979062637
the regulatory subunits of fission yeast protein phosphatase 2a (pp2a) affect cell morphogenesis, cell wall synthesis and cytokinesis.protein phosphatase 2a (pp2a) holoenzymes have a trimeric structure, consisting of a catalytic subunit c and two regulatory subunits a (pr65) and b (pr55). in fission yeast the c subunits, being 80% identical to their mammalian counterparts, are essential for viability and negatively regulate the entry into mitosis. genetic analyses in budding yeast and drosophila show that the regulatory subunits are implicated in chromosome segregation, cell morphogenesis and/or cytokinesis.19969078365
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