Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cell cycle-dependent chronic infection of human cytomegalovirus in human osteogenic sarcoma cells. | human osteogenic sarcoma cells transformed by murine sarcoma virus (r970) showed restricted growth of human cytomegalovirus (hcmv). virus was attached to the same extent as in human fibroblasts. hcmv growth was blocked at early stages after virus penetration. splitting of infected r970 cultures resulted in infection of all cells. in experiments using synchronized r970 cells it was found that factor(s) associated with the s-phase of the cell cycle might be necessary for establishment of the infec ... | 1979 | 230311 |
| supplemental arginine increases thymic cellularity in normal and murine sarcoma virus-inoculated mice and increases the resistance to murine sarcoma virus tumor. | arginine supplements were given to 6 week old cba mice beginning 3 days prior to inoculation with a murine sarcoma virus, the moloney sarcoma virus (msv). although the basal diet contained 1.8% arginine and was therefore not arginine-deficient, supplementation of the diet and the drinking water with 0.5% arginine hcl reduced tumor incidence, lengthened the latency period, decreased tumor size, and hastened tumor regression. arginine also increased thymic weight and cellularity in normal and in m ... | 1979 | 231121 |
| inhibition of murine sarcoma virus induced transformation in an adenovirus -- nrk system. | cell transformation induced by murine sarcoma virus (msv-m) is significantly inhibited (80--90%) in a clonal line of normal rat kidney (nrk) cells when they are infected with rat cell passaged adenovirus 12 (r-ad12). no inhibition is seen when r-ad12 is added simultaneously with, or 1 1/2 or 24 hr after, msv-m infection, suggesting that inhibition occurs most probably intracellularly. there is also a direct correlation between the extent of focus formation and the concentration of r-ad12 used. c ... | 1979 | 231569 |
| decrese of collagen biosynthesis ability of swiss 3t3 cells transformed with the murine sarcoma virus (msv). | 1979 | 232424 | |
| functional heterogeneity and t cell-dependent activation of macrophages from murine sarcoma virus (msv)-induced tumors. | in studies on the functional activity of macrophages isolated from murine sarcoma virus (msv)-induced tumors, we have found that these cells may suppress immune responses as well as act as effector cells against the tumor. previously, we reported that macrophages from the tumor could inhibit the antitumor response by suppressing proliferation-dependent immune functions. here, we demonstrate that macrophages can also suppress the production of migration inhibition factor (mif) and macrophage acti ... | 1979 | 232622 |
| enhancement of tumor induction in rats with moloney murine sarcoma virus by a "new" method based on direct injection into fetuses. | undiluted, fivefold-diluted, and 25-fold-diluted doses of a stock of moloney murine sarcoma virus were injected directly, in a volume of 0.025 ml, into the backs of fetal sprague-dawley rats by laparotomy through the uterine wall at 18 days of gestation. during the first 8 weeks after birth the young responded to the virus with remarkably high but dose-dependent incidences of neoplasms. when a one-fifth dilution of the virus preparation was inoculated at fetal ages 16, 18, and 20 days, the incid ... | 1977 | 264591 |
| intratibial moloney sarcoma virus-induced osteosarcoma in the rat: tumor incidence and pathologic evaluation. | osteosarcomas were induced in approximately 80% of young new zealand black rats by the intratibial inoculation of moloney murine sarcoma virus from day 1 to day 5 after birth. the neoplasms were composed of a spectrum of well-differentiated to poorly differentiated osteoblasts, osteocytes, and osteoclasts. budding of c-type viral particles was associated with tumor induction. compared to rats inoculated on day 1 after birth, rats inoculated at 4 days of age developed consistently more osteoproli ... | 1977 | 264594 |
| cleavage map of linear mouse sarcoma virus dna. | proviral dna transcribed from the rna of moloney murine sarcoma virus was isolated from newly infected cells. three forms of viral dna were observed: (i) a linear double-stranded form of 3.4 x 10(6) daltons which constituted the major viral dna species in the cell, and is thought to be a complete transcript (monomer) of viral rna; (ii) a fast-sedimenting viral dna bigger than the monomeric unit which can be either integrated provirls or concatamers; and (iii) covalently closed circles of monomer ... | 1977 | 264682 |
| characterization of human cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine. | human osteosarcoma (hos) clonal cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) were characterized, and compared to non-producer hos cells transformed by kirsten murine sarcoma virus (ki-msv). the mnng- and virus-transformed cells grew in the aggregate form above an agar base, grew in soft agar, and had a high fibrinolytic activity. when inoculated into nude mice, all the chemically or virally altered cells produced tumors or tumor nodules. when transplanted into ats-tr ... | 1977 | 265298 |
| pathology of neoplasms and other lesions induced in rats with moloney murine sarcoma virus. | moloney murine sarcoma virus (m-msv) was injected directly into the fetuses of sprague-dawley rats during the late stage of gestation and into the neonates within 24 hours after birth. ninety rats developed 188 neoplastic lesions during the 8-week period of observation. nearly all of the neoplasms were of mesenchymal derivation. sixty percent of these neoplasms revealed more complex histologic features than those previously reported for neoplasms induced in rodents with m-msv and were designated ... | 1977 | 266356 |
| moloney murine sarcoma virions synthesize full-genome-length double-stranded dna in vitro. | moloney murine sarcoma virus (msv) virions incubated under optimal conditions were shown to support extensive synthesis of double-stranded dna. the major product, a 5950-base-pair (6-kilobase-pair dna) double-stranded dna, was characterized by cleavage with restriction endonucleases and shown to contain a 600-nucleotide-long direct repeat at both ends of the msv genome. linear dna molecules made in vivo shortly after infection were compared to the linear double-stranded dna synthesized in vitro. ... | 1979 | 291003 |
| murine sarcoma virus (msv)-induced tumors in mice. i. distribution of msv-immune cytolytic t lymphocytes in vivo. | quantitative studies using a 51cr release assay were performed to analyse the time-course of appearance and specificity of cytolytic cells in c57bl/6 mice inoculated with moloney murine sarcoma virus (msv). various lymphoid organs were studied including spleen, mesenteric lymph nodes (mln), lymph nodes regional to the msv-induced tumor (rln), and peripheral blood. furthermore, the kinetics of appearance of cytolytic cells within the msv-induced tumor was determined. in agreement with previous st ... | 1977 | 300075 |
| t and b lymphocyte migration into syngeneic tumors. | the migration of splenic t and b lymphocytes into syngeneic tumors undergoing immunologic rejection was investigates. spleen cells were obtained from normal balc/c mice or balb/c mice bearing tumors induced by murine sarcoma virus (msv). either whole spleen cells or immunoabsorbent purified t and b cells were radiolabeled with sodium chromate-51 and injected i.v. into normal or msv inducted-tumor bearing syngeneic recipients. twenty-four hours later the recipient mice were sacrificed and radioac ... | 1977 | 300384 |
| epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (egf) receptors and the effect of mouse sarcoma virus transformation. | fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, nrk. these clones were studied for their ability to bind epidermal growth factor (egf), susceptibility to transformation by mouse sarcoma virus (msv), and alteration in egf binding upon sarcoma virus transformation. the epithelioid clones bound much more egf than the fibroblastic clones; scatchard plots on two of these clones, one epithelioid and one fibroblastic, showed that the higher egf binding (1.3 x 10( ... | 1978 | 304450 |
| suppression of lymphokine production by macrophages infiltrating murine virus-induced tumors. | immune spleen cells from mice injected with murine sarcoma virus (msv) can produce migration inhibition factor (mif) in vitro after stimulation with intact tumor cells. we have now found that this effector function can be regulated by suppressor macrophages present in the tumor. the cells from the tumor (cft) exert a strong suppressive effect even after treatment with anti-thy 1.2 plus complement but lose this capacity if adherent or phagocytic cells are removed, suggesting that the macrophage i ... | 1979 | 314424 |
| in vitro studies of cell-mediated immunity to moloney murine leukemia virus and moloney leukemia-associated surface antigens. | cell-mediated immunity to moloney murine leukemia virus (m-mulv) and to tumor-associated surface antigens of leukemia cells induced by the virus was studied with an in vitro migration inhibition factor assay. spleen cells of c57bl/6n mice at day 14 following inoculation with moloney murine sarcoma virus, produced migration inhibition factor in response to m-mulv. the moloney murine sarcoma virus-immune spleen cells, however, did not respond to other murine type c viruses, to akr and rauscher vir ... | 1979 | 387218 |
| molecular cloning of moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. | the unintegrated circular dna form of moloney murine sarcoma virus (msv) has been cloned in bacteriophage lambda. discrete deletions in the viral genome were shown to occur during propagation of recombinant phage in escherichia coli. heteroduplex and restriction enzyme analyses indicated the deletion of tandemly repeated sequences within certain of the cloned msv dna inserts. cloned msv dna was used to prepare a probe composed of its acquired cellular (src) sequences, shown previously to be nece ... | 1979 | 392518 |
| chemical transformation of human revertant cells induced by murine sarcoma virus. | a human revertant cell line, derived from non-producer human osteosarcoma cells (np/khos) induced by kirsten murine sarcoma virus, was treated in vitro with various levels of polycyclic aromatic hydrocarbons (3-methylcholanthrene, 7,12-dimethylbenz(alpha)anthracene, and benzo(alpha)pyrene [bp] or dimethyl sulfoxide (control). cells treated only with 3-methylcholanthrene and 7,12-dimethylbenz(alpha)anthracene underwent morphological alteration in vitro, and produced tumors when injected into nih ... | 1978 | 415010 |
| "sarc" sequence transcription in moloney sarcoma virus-transformed nonproducer cell lines. | (3)h-labeled complementary dna(sarc), complementary to the murine sarcoma virus (msv)-specific portion of the moloney msv (m-msv) genome, was prepared. m-msv-specific rna was then quantitated in the cytoplasm of several m-msv-transformed, non-virus-producing, clonal nih 3t3 cell lines. these lines, designated 71 n clones 5, 6, and 3, have been characterized previously by the degree to which they exhibit transformation properties and transcribe moloney murine leukemia virus-related rna (s. salzbe ... | 1979 | 430602 |
| enhancement of experimental tumors in mice by treatment with concanavalin a. | weanling balb/c mice given injections of 300 micrograms concanavalin a (con a) prior to and at frequent intervals after challenge with moloney murine sarcoma virus (m-musv), 3-methylcholanthrene (mca), or tepc-15 plasmacytoma cells showed an enhancement of tumor induction or development. with the m-musv and mca systems, this enhancement was evidenced by larger tumors and, in the mca system, by more devastating tumors. regression of the m-musv-induced tumors was more prolonged in con a-treated mi ... | 1979 | 480379 |
| prevention of transplantable tumors by adoptive transfer of spleen cells from immunized rats. | fischer 344 rats were specifically hyperimmunized with allogeneic, nonvirus-producing [kirsten murine sarcoma virus (kimsv)] or syngeneic, virus-producing [kimsv (rasheed)] rat tumors. spleen cells taken from these rats adoptively transferred protection against a 100 to 1,000 x rat tumor dose50 cell challenge with several different transplantable rat tumors. protection was obtained with spleen cells after removal of adherent cells and macrophages but not peritoneal cells. the spleen cells were n ... | 1979 | 501086 |
| the transforming gene of moloney murine sarcoma virus. | a cleavage map of the moloney murine sarcoma viral dna was constructed and compared with that of a spontaneously occurring deletion mutant. by restriction enzyme analysis, it was shown that a region encompassing over 40% of the viral information was not essential for transformation or rescue of the deletion mutant. the transforming region was further localised by analysis of the transforming activity in tissue culture of isolated restriction fragments of linear duoble-stranded sarcoma viral dna. ... | 1979 | 503218 |
| immunochemical characterization of tumor-associated surface antigens on a moloney leukemia virus-lymphoma, mbl-2. | tumor-associated surface antigens (tasa) on a moloney leukemia virus (m-mulv)-induced lymphoma, mbl-2, in c57bl/6 mice (b6) were characterized. the surface proteins of mbl-2 cells were selectively radioiodinated and then extracted by nonidet p40. the solubilized materials were then reacted with a variety of antisera: monospecific antisera to murine leukemia viral proteins (anti-gp69/71, anti-p30, anti-p15, anti-p12 and anti-p10), sera from b6 which regressed murine sarcoma tumors induced by muri ... | 1979 | 528073 |
| activity of 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride against experimental tumors in mice and rats. | 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride (ciq) was shown to exert significant antitumor activity against the ehrlich carcinoma and sarcoma 180 transplantable tumors in mice by the intraperitoneal (ip) or oral (po) routes and when incorporated into diet. a solid tumor induced in balb/c mice by the subcutaneous (sc) implantation of nonproducer murine sarcoma virus-transformed balb/3t3 cells was also inhibited by ciq after ip or po treatment but there wa ... | 1978 | 579626 |
| anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation. | a viral inhibitor(s) is released in the supernate of mixed cultures containing human or mouse lymphocytes and cells from certain lines. the inhibitor is active against a variety of unrelated viruses and is a protein that is not toxic for cells. it does not inactivate viruses directly, but inhibits viral replication through an intracellular mechanism that involves synthesis by the cells of both rna and protein. these characteristics identify the inhibitor as an interferon. the anti-viral activity ... | 1978 | 650155 |
| in situ fc receptor-bearing cells in two murine tumors. i. isolation and identification. | the in situ inflammatory response was studied in 2 different tumors, the t1699 mammary adenocarcinoma in inbred dba/2 mice and a sarcoma induced in inbred c57bl/6 mice by murine sarcoma virus. the presence of fc receptors (fcr's) on infiltrating host cells was determined by rosette formation with antibody-coated sheep red blood cells (ea). differences in fcr-binding activity among various cells were detected with the use of ea prepared with a range of antibody concentrations. cells bearing fcr r ... | 1978 | 650702 |
| effects of hydroxyurea on murine type c virus-specific dna synthesis in newly infected cells. | cell transformation and replication of the rauscher pseudotype of moloney murine sarcoma virus in mouse embryo fibroblasts were inhibited by hydroxyurea within a critical time period of 30 to 90 min postinfection. in cells infected by rauscher leukemia virus, treatment with 1mm hydroxyurea during the critical time period resulted in the accumulation of minus-strand dna (molecular weight, 3 x 10(6)) in association with the parental viral genoma rna. this 5 to 6 x 10(6) dalton rna:dna hybrid was f ... | 1978 | 657143 |
| protective effect of murine sarcoma virus-superinfected mouse tumor cells against outgrowth of corresponding noninfected tumor. | cultured lines of three newly established methylcholanthrene-induced tumors, mbk and mbl in cba and mc57m in c57bl mice, and two mammary tumors, sbfnhc in cba and s3w in asw mice, were superinfected in vitro with moloney sarcoma or leukemia virus (msv, mlv). after superinfection, they expressed the moloney virus-determined cell surface antigen (mcsa) and murine c-type viral p30 antigen, and produced nb-tropic c-type virus. the virus-infected tumors became more rejectable in normally susceptible ... | 1978 | 705875 |
| comparative immunological studies on cryosurgery and surgical operation using moloney murine sarcoma virus-induced primary tumors in balb/c mice. | the effect of cryosurgery and surgical operation on moloney murine sarcoma virus-induced primary tumors in the treated and untreated groups of balb/c mice was compared in terms of tumor growth, cumulative mortality, lymphocyte-mediated cytotoxicity, proliferative response of lymphocytes, and humoral antibody formation. the results indicate that cryosurgical treatment showed both in vivo and in vitro effects; (i) tumor growth and cumulative mortality in mice treated by cryosurgery were significan ... | 1978 | 710802 |
| cytolytic and cytostatic anti-tumor activities of macrophages from mice injected with murine sarcoma virus. | 1979 | 759378 | |
| standardization of the chromium-51 release, cell-mediated cytotoxicity assay: cryopreservation of mouse effector and target cells. | utilizing controlled cryopreservation techniques, we were able to standardize the 51cr release cytotoxicity assay and thereby ensured reliable comparisons between results obtained on different days. optimal conditions for freezing of both effector and target cells were quite similar. dimethyl sulfoxide (dmso) at a concentration of 7.5-10.0% was employed as the cryoprotective agent and cells were frozen at the rate of -1 degrees c/minute. the handling procedures for the cells before and after fre ... | 1977 | 839557 |
| a heteroduplex study of the sequence relationships between the rnas of m-msv and m-mlv. | the regions of sequence homology between the rna genomes of a murine sarcoma virus (clone 124 moloney-msv) and its parental helper virus (clone 3 moloney-murine leukemia virus (m-mlv)) have been mapped. long complementary dna transcripts of the m-mlv rna were hybridized to m-msv rna, and the structures of the hybrids were observed in the electron microscope. beginning at the 5' end, the two rnas are homologous for a region of length 2.25 kb (kilobases). in the next region, of length approximatel ... | 1977 | 844103 |
| augmentation of natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic target cells. | the levels of natural cell-mediated cytotoxicity against tumor cells in young balb/c and balb/c nude mice could be augmented by inoculation of a variety of mouse tumor cells or of mouse thymocytes. in older mice with low levels of spontaneous cytotoxic reactivity, inoculation of tumor cells led to rapid appearance of cytotoxicity. this augmented cytotoxicity reached a peak 3 days after inoculation and then declined rapidly. the specificity of the augmented cytotoxicity appeared to be the same as ... | 1977 | 844921 |
| defective friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. | defective friend spleen focus-forming virus (sffv) is able to interfere with the ability of its naturally occurring leukemia-inducing helper virus (llv-f) to induce xc plaque formation in several different strains of mouse embryo cells. this interference has been observed by using two different sffv preparations, one contained in an nb-tropic stock of friend virus (fv) complex, and the second present in a c57bl-adapted strain of fv complex containing an associated b-tropic llv-f helper. the llv- ... | 1977 | 904028 |
| phenotypic markers in human skin fibroblasts as possible diagnostic indices of hereditary adenomatosis of the colon and rectum. | hereditary adenomatosis of the colon and rectum (acr) and its gardner's syndrome variant, an autosomal dominant trait, indicate a propensity for neoplasia. the present study describes the growth abnormalities of cultured human skin fibroblasts derived from normal-appearing cutaneous biopsies of acr genotypes and a portion of the clinically asymptomatic acr progeny, first filial generation, and their differential susceptibility to transformation by kirsten murine sarcoma virus. these skin fibrobl ... | 1977 | 922693 |
| immunogenic properties of a nonproducer malignant tumor induced by murine sarcoma virus. | a cloned cell line h-11 (hp) derived from an msv-induced neoplasm (a hemangiosarcoma) was found to possess virus-specific tumor rejection antigen(s). the specific nature of the immune response was established through the use of a polyoma virus-induced neoplasm no. 89 both in cross immunization and cross challenge experiments. virus antigens or surface antigens associated with virus production are not responsible for tumor rejection since virus production could not be detected, nor were any viral ... | 1976 | 967890 |
| malignant transformation and viral replication of rat bone and muscle cells after in vitro infection with rat-adapted murine sarcoma virus (moloney). | isolated bone and muscle cells from rat fetuses were infected with the rat-adapted osteosarcomogenic murine sarcoma virus (moloney) for examination of malignant transformation and viral replication. after the infection, the bone cells underwent an unusual transformation characterized by two patients in the focus formation. in the early transformation (transformation i), foci consisting of only morphologically altered cells appeared, but they soon disappeared. focus formation of this type continu ... | 1976 | 975041 |
| suppression of moloney sarcoma virus immunity following sensitization with attenuated virus. | murine sarcoma virus (moloney strain) (msv-m)-induced tumors are unusual in that they regularly appear less than 2 weeks after virus inoculation, progress for 1 to 2 weeks, and are rejected by normal adult balb/c mice. rejectio leaves the animals immune to tumor induction. in the present study, presensitization of normal adult balb/c mice with attenuated msv-m resulted in an altered pattern of tumor immunity. injection of active msv-m into the presensitized animals resulted in tumor induction an ... | 1976 | 1000500 |
| reduced transplantability of syngenic mouse tumors superinfected with membrane viruses in nu/nu mice. | transplantability of mouse tumors superinfected with various kinds of membrane viruses was investigated in syngeneic hosts. methylcholanthrene-induced fibrosarcomas in balb/c mice, meth a, and in c57bl/6 mice, bmt-, superinfected with friend lymphatic leukemia virus in mice given neonatal injection of the virus, grew more slowly than uninfected tumors. the retardation of growths was not observed in mice that had been given injections of the virus at birth. similarly, meth a and a hepatoma in c3h ... | 1976 | 1006777 |
| search for nucleic acid sequences complementary to a murine oncornaviral genome in poly(a)-rich rna of human leukemic cells. | the presence of viral-like sequences in the rna of various types of leukemic cells was investigated by hybridizing cellular poly(a)-containing rna with cdna synthesized in an endogenous system of purified moloney murine sarcoma virus [m-msv-(mlv)]. poly(a)-rna-cdna hybrids were detected by assaying their resistance to s1 nuclease. hybrids were found in 22 out of the 46 leukemias that were tested. none of the controls, including material obtained from buffy coats, bone marrow cells, and a continu ... | 1975 | 1061078 |
| virus-induced osteosarcoma in rats. | an experimental model for osteosarcoma was developed in which, after moloney murine sarcoma virus was injected into the tibial marrow space of three strains of inbred neonatal rats, a highly malignant neoplasm arose within ten days. this tumor was readily maintained in tissue culture and was transplantable to adult rats. it arose in the metaphyseal marrow of several bones, was locally invasive, metastasized to the lungs, and histologically resembled osteosarcoma. | 1976 | 1063127 |
| immunogenicity of virus-induced rat osteosarcoma. | the immunogenicity of a virus-induced rat osteosarcoma was studied utilizing the lymphocyte microcytotoxicity test. intratibial injection of murine sarcoma virus (moloney) resulted in the development of palpable tumors at the injection site which on histopathological examination appeared to be osteosarcomas. in 73 per cent of animals injected these tumors progressed and metastasized to the lungs. lymphocytes from these "progressor" animals demonstrated an ability to kill osteosarcoma cells in vi ... | 1976 | 1063128 |
| tumor induction by moloney sarcoma virus in athymic nude mice. | athymic nude (nu/nu) mice, normal nmri (plus/plus) mice, and nu/plus heterozygotes, inoculated at birth with the moloney strain of murine sarcoma virus (m-msv), did not differ either in tumor growth rate or incidence or in mortality due to tumor development; neither did nude (nu/nu) and normal nmri mice inoculated with m-msv at 30 days of age differ in the initial tumor growth rate. however, tumors induced in adult nude mice grew progressively and eventually killed the hosts, whereas tumors indu ... | 1975 | 1078700 |
| two functionally distinct anti-tumor effector cells isolated from primary murine sarcoma virus-induced tumors. | the ability of cells from primary msv-induced tumors to function as effector cells in vitro was evaluated. host cells were isolated by enzymatic disaggregation of the tumor and fractionated by sedimentation velocity at unit gravity on a ficoll gradient. characterization of these cells indicated that 30 to 40 % were t lymphocytes, about 50% were macrophages and less than 5% were b lymphocytes. two different functional activities were mediated by these cells: cytolysis, as measured by the cra, and ... | 1976 | 1084897 |
| studies on the distribution and origin of cytolytic t lymphocytes present in mice bearing moloney murine sarcoma virus (msv)-induced tumors. | 1975 | 1085620 | |
| cell-mediated immunity to moloney sarcoma virus in mice. ii. analysis of antigenic specificities involved in t lymphocyte-mediated in vivo rejection of murine sarcoma virus-induced tumors. | sublethally irradiated balb/c mice innoculated with moloney sarcoma virus (msvm) develop progressively growing tumors and die within 30 days of virus innoculation. these animals can be protected from tumor progression (and death) by innoculation of small numbers of msv-immune t lymphocytes from msv-m innoculated (but unirradiated) animals. t lymphocytes in these donor animals have been shown to express immunity to a variety of viral and virally-induced antigens. we have investigated whether immu ... | 1975 | 1086221 |
| delayed tumour appearance and absence of regression in nude mice infected with murine sarcoma virus. | 1975 | 1110765 | |
| antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of igm and igg with specificity for mlv determined antigen(s). | antisera with specificity for moloney leukemia virus-(mlv) determined antigen(s) were studied for their ability to induce mlv antigen bearing target cell reduction by lymphocytes in microcytotoxicity assays. sera from animals which had regressed moloney sarcoma virus (msv) tumors as well as sera from animals with progressively growing msv tumors were found to induce normal lymphocytes to be active against the targets. regressor serum was found also to induce cytotoxicity by immune lymphocytes fr ... | 1975 | 1117138 |
| some pathogenic aspects of murine sarcoma virus (moloney) in rats. | the oncogenic effect of the murine sarcoma virus (moloney) was tested in rats. host-age and virus-dose dependent variations in the pathogenic spectrum were found. four tumour "stages" were described: progressively lethal; lethal after long stationary period; complete tumour regression; tumour reoccurrence after complete regression. virus recovery was highest from the progressively growing tumours and in the reoccurred neoplasms, while no virus was found in stationary tumours or in those sites ... | 1975 | 1117696 |
| murine sarcoma virus related nucleic acid sequences in a non-transforming virus derived from an interspecies pseudotype sarcoma virus. | a number of sarcomagenic viruses recovered from hamster tumors induced originally by moloney sarcoma virus or several of its mouse pseudotypes appear to be interspecies pseudotypes with structural proteins of hamster type-c viruses and nucleic acid sequences of both mouse and hamster specificity. a non-transforming virus (gloh-minus) recovered from a sarcomagenic virus preparation obtained from a tumor induced by the gross pseudotype of moloney sarcoma virus was previously shown to retain mouse ... | 1975 | 1140876 |
| [histopathologic and ultrastructural study of tumors induced by murine sarcoma virus (msv) (author's transl)]. | various growth phases of moloney murine sarcoma virus (m-msv) induced tumors in suckling and young adult balb/c mice have been studied by light and electron microscopy. in the early phase (3-6 days following m-msv), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema "activated" muscle satellite, cells, endothelial cells and fibroblasts, scattered type c virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis an ... | 1975 | 1226574 |
| mixed lymphocyte/tumor-cell interaction in a murine sarcoma virus (moloney)-induced tumor system. comparison between lymphoproliferation and lymphocyte cytotoxicity. | studies of the mlti were performed in a primary msv-induced tumor system. reactivity against mitomycin-c-treated rbl-5 cells could be detected during a limited period at about 14 days after virus injection. the degree of reactivity of msv 14 spleen cells was quite variable; stimulation indices ranged from 2.2 to 9.4. activity in the mlti appeared to a great extent to be dependent on t-lymphocytes. a relatively broad spectrum of specificity was demonstrated in experiments using additional stimula ... | 1976 | 1254360 |
| inhibition of cell-mediated cytotoxicity against tumor-associated antigens by suppressor cells from tumor-bearing mice. | spleen cells from mice bearing primary tumours induced by moloney strain of murine sarcoma virus (m-musv) strongly inhibited the in vitro generation of specific secondary cell-mediated cytotoxic response of spleen cells from m-musv regressor mice. these suppressor cells were resistant to treatment with anti-theta serum and complement or to x-irradiation. it appeared that suppressor cells may have had a role in limiting the host's immune response against tumor growth. | 1976 | 1255803 |
| elimination of the sarcoma genome from murine sarcoma virus transformed cat cells. | cat cells transformed by moloney murine sarcoma virus contain virus-specific sequences in their rna and dna. cloned, spontaneous revertant cell lines derived from clones of these cells had no evidence of the sarcoma genome in the cell rna or dna as judged by rna-complementary dna or dna-complementary dna hybridizations. ?this is apparently the first report of loss of a transforming genome in a revertant cell line. | 1976 | 1257745 |
| on the early emergence of reverse transcription: theoretical basis and experimental evidence. | reverse transcriptase (rt) was first discovered as an essential catalyst in the biological cycle of retroviruses. however, in the past years evidence has accumulated showing that rts are involved in a surprisingly large number of rna-mediated transpositional events that include both viral and nonviral genetic entities. although it is probable that some rt-bearing genetic elements like the different types of aids viruses and the mammalian line family have arisen in recent geological times, the po ... | 1992 | 1282161 |
| mechanism of anti-human immunodeficiency virus action of polyoxometalates, a class of broad-spectrum antiviral agents. | various polyoxometalates proved inhibitory to the replication of a number of enveloped dna and rna viruses, i.e., herpesviruses (herpes simplex and cytomegalo), togaviruses (sindbis), paramyxoviruses (respiratory syncytial), rhabdoviruses (vesicular stomatitis), arenaviruses (junin and tacaribe), and retroviruses [human immunodeficiency virus type 1 (hiv-1) and type 2 (hiv-2), simian immunodeficiency virus, and murine sarcoma virus]. the most potent compounds, i.e., jm1590 [k13[ce(siw11o39)2]. 2 ... | 1992 | 1282664 |
| v-mos proteins encoded by myeloproliferative sarcoma virus and its ts159 mutant. | the myeloproliferative sarcoma virus (mpsv) v-mos protein was predicted to be identical in size to p39c-mos because of an observed one-base deletion in the seventh codon of the env-mos open reading frame, which would allow translation to initiate at the methionine equivalent to codon 32 of the env-mos gene. on the basis of published results, p39c-mos is known to have greatly reduced in vitro protein kinase activity compared with p37env-mos encoded by moloney murine sarcoma virus. unexpectedly, t ... | 1992 | 1309903 |
| inhibitory effect of myristylation on transrepression by fbr (gag-fos) protein. | the myristylated v-fos product, fbr murine sarcoma virus (gag-fos) protein, exhibits a lower level of transrepression of the serum response element (sre) than does c-fos protein (fos). mutation of the n-terminal myristylation site in fbr protein restored sre transrepression. replacement of n-terminal viral gag sequences with the fos n terminus also restored this activity, providing additional evidence that myristylation inhibits transrepression by fbr protein. however, the myristylated gag domai ... | 1992 | 1310154 |
| evidence for interaction between v-mos and a p34cdc2 isoform, p35cdk. | the c-mos gene product (c-mos) encodes a serine/threonine kinase required for activation of pre-mpf (maturation-promoting factor) to mpf in oocytes undergoing meiosis and also for stabilization of mpf leading to metaphase arrest in unfertilized eggs. in order to determine whether the v-mos gene product (v-mos) causes neoplastic transformation via interaction with cell cycle control elements, we have searched for proteins that interact with v-mos. extracts of nih3t3 cells transformed by v-mos enc ... | 1992 | 1323818 |
| expression of gene-specific rna in cultured cells exposed to rotating 60-hz magnetic fields. | replicate sets of cultures of mouse and of human cells were exposed to cyclic, sine wave, 60-hz rotating magnetic fields of 1.0 gs (1 gs [symbol: see text] 0.1 mt) for 24-, 48-, or 72-h periods. total rna extracted from unexposed control and from magnetic field exposed cells was dot blot hybridized to a number of oncogene probes (including v-myc, v-fos, v-raf, and v-ha-ras), a probe for 3611 murine sarcoma virus, a probe for the 70,000 dalton heat shock protein (hsp70), and a probe for the long ... | 1992 | 1325160 |
| expression and characterization of recombinant human protein s in heterologous cells--studies of the interaction of amino acid residues leu-608 to glu-612 with human c4b-binding protein. | mouse c127 epithelioid cells were genetically engineered to produce biologically active gamma-carboxylated human protein s. a full length human protein s cdna was cloned into a bovine papilloma virus (bpv) based shuttle vector under the transcriptional control of the moloney murine sarcoma virus enhancer and the mouse metallothionein promoter. stable expression was obtained in transfected c127 cells. expression of gamma-carboxylated protein s was dependent on the presence of vitamin k in the cul ... | 1992 | 1325680 |
| genomic structure and expression of the human fau gene: encoding the ribosomal protein s30 fused to a ubiquitin-like protein. | the fau gene is the cellular homolog of the fox sequence in the finkel-biskis-reilly murine sarcoma virus (fbr-musv). this virus acquired the fau sequence in its reversed transcriptional orientation. human and mouse fau cdna's were identified and both encode a new protein of 133 aa. we show that fau (for fbr-musv associated ubiquitiously expressed gene) becomes expressed in all different tissues tested as a 600 bp messenger and we report the genomic structure of the human fau gene. the gene cons ... | 1992 | 1326960 |
| pheochromocytomas and c-cell thyroid neoplasms in transgenic c-mos mice: a model for the human multiple endocrine neoplasia type 2 syndrome. | transgenic mice carrying and expressing a mos protooncogene, linked to the moloney murine sarcoma virus long terminal repeat, develop severe neurological defects and lens abnormalities. here we report that after long latent periods, mice in three of four of these mos transgenic lines develop a high frequency of multicentric pheochromocytomas and/or medullary thyroid neoplasms. the pattern of tumor formation is remarkably similar to the human autosomal dominantly inherited neoplastic syndrome, mu ... | 1992 | 1345812 |
| in vitro differentiation of the human osteosarcoma cell lines, hos and khos. | the osteogenic potential of the two human osteosarcoma cell lines hos and khos; a cell line produced by the transformation of the hos cells by the kirsten murine sarcoma virus, was studied in vitro. hos cells cultured more than 2 weeks formed nodules composed of two morphologically distinct layers, an epithelial-like surface cell layer and a collagen-rich inner cell layer. alkaline phosphatase (alpase) activity occurred in the plasma membrane of the surface cell layer, and calcified substances d ... | 1992 | 1357821 |
| molecular cloning and characterization of v-mos-activated transformation-associated proteins. | using monoclonal antibodies we previously detected two forms of transformation-associated proteins, a 64-kda protein and a 68-kda protein, in temperature-sensitive 110-moloney murine sarcoma virus-mutant-transformed rat kidney 6m2 cells. the identity and functions of the transformation-associated proteins were previously unknown. by molecular cloning techniques and immunoscreening, we have isolated two cdna clones (34a and 79b3) that were found by western blot analysis to code for a monoclonal a ... | 1992 | 1370458 |
| both p21ras and pp60v-src are required, but neither alone is sufficient, to activate the raf-1 kinase. | the raf genes encode a family of cytoplasmic proteins with intrinsic protein-serine/threonine kinase activity. the c-raf gene is the cellular homolog of v-raf, the transforming gene of murine sarcoma virus 3611. the constitutive kinase activity of the v-raf protein has been implicated in transformation and mitogenesis. the activity of raf-1, the protein product of the c-raf gene, is normally suppressed by a regulatory n-terminal domain. activation of various tyrosine-kinase growth factor recepto ... | 1992 | 1372995 |
| midline brain tumors in msv-sv 40-transgenic mice originate from the pineal organ. | adult transgenic mice expressing the large t-antigen of the simian virus 40 (sv 40) under the control of the moloney murine sarcoma virus (msv) enhancer and the sv 40 promoter develop inheritable uniform midline brain neoplasms showing features of primitive neuroectodermal tumors. the origin and histogenesis of these tumors were investigated in the present study. the brain and pineal organ of fetal and young transgenic mice less than 3 months old displayed normal macroscopic and microscopic feat ... | 1992 | 1373025 |
| intermediate filaments in rat ovarian surface epithelial cells: changes with neoplastic progression in culture. | interrelationships between neoplastic progression and the expression of intermediate filaments were examined in primary cultures, immortal lines, and kirsten murine sarcoma virus (kimsv) transformed lines of rat ovarian surface epithelial (rose) cells. immunofluorescence microscopy revealed abundant keratin filaments in all cells of primary cultures. in immortal, nontumorigenic lines, keratin filaments were detected in fewer cells, in smaller numbers, and in microscopically altered forms. the pe ... | 1992 | 1374615 |
| 2',5'-bis-o-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino-1'',2''- oxathiole-2'',2'-dioxide)pyrimidine (tsao) nucleoside analogues: highlyselective inhibitors of human immunodeficiency virus type 1 that are targeted at the viral reverse transcriptase. | a series of pyrimidine nucleoside analogues containing [2',5'-bis-o-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino- 1'',2''-oxathiole-2'',2''-dioxide)]-beta-d-ribofuranose as the pentose were found to inhibit human immunodeficiency virus type 1 [hiv-1(iiib)] replication at a concentration of 0.06-0.8 microm but were not cytotoxic at a 1000- to 10,000-fold higher concentration. these nucleoside derivatives were also effective against various other hiv-1 strains, including those resistant to 3' ... | 1992 | 1374900 |
| karyotype instability and altered differentiation of rat sarcoma cells after retroviral infection. | the karyotypic and phenotypic stability of cultured rat fibrosarcoma cells was challenged by infection with moloney murine sarcoma virus (momusv). after transformation, the spindle-like morphology of the parental hh-16 cl.2/1 cells had altered to a rounded phenotype, which was maintained in tumors produced by inoculating transformed cells into congenic animals. in contrast to the parental cells, transformed cells lacked cables of cytokeratins 14-16 and 19 and showed reduction of the mesenchymal ... | 1992 | 1377009 |
| reversal of divergent differentiation by ras oncogene-mediated transformation. | in embryogenesis, ovarian surface epithelial cells and ovarian granulosa cells arise through divergent differentiation from a common mesenchymal precursor, the urogenital ridge. in the adult rat, ovarian surface epithelial cells are nonsteroidogenic and keratin positive, while ovarian granulosa cells are steroidogenic and keratin negative. in culture, kirsten murine sarcoma virus-transformed, tumorigenic ovarian surface epithelial cells continued to express keratin but also became steroidogenic. ... | 1992 | 1379124 |
| depressed interferon synthesis in skin fibroblasts from lung cancer patients. | skin fibroblast cell cultures, derived from male adult lung cancer patients, an adult control population, and a newborn population were examined for their susceptibility to transformation with kirsten murine sarcoma virus and their ability to respond to an interferon inducer (poly i.poly c). an association between sensitivity to viral transformation and induction of interferon was observed. cultures derived from lung cancer patients demonstrated an increased sensitivity to virus transformation a ... | 1992 | 1379289 |
| role of adhesion molecules in the immune reaction to m-msv-induced tumors. | we have investigated the in vivo role of 2 different adhesion molecules, lfa-1 and lecam-1, in the immune reaction to moloney-murine-sarcoma-virus(m-msv)-induced tumors, which undergo a peculiar spontaneous regression due to generation of a strong virus-specific cytotoxic-t-lymphocyte(ctl) response. repeated administration of anti-lfa-1 monoclonal antibody (fd441.8 mab), i.p. or at the site of virus inoculation, enhanced tumor growth and delayed regression, while i.p. administration of anti-leca ... | 1992 | 1385341 |
| nucleotide sequence and tissue distribution of mouse transforming growth factor-alpha. | transforming growth factor-alpha (tgf alpha) is secreted into the medium of the moloney murine sarcoma virus-transformed 3t3 cell line, 3b11-1c. using reverse transcription-polymerase chain reaction (rt-pcr), we have amplified, cloned and sequenced a cdna fragment from this cell line which encodes the full protein-coding region of the mouse tgf alpha precursor. the deduced amino acid sequence (159 residues) differs from that of human tgf alpha at 13 sites and from that of rat at 3 sites. in the ... | 1992 | 1420315 |
| regulation of the efficiency and thermodependence of murine sarcoma virus musvts110 rna splicing by sequences in both exons. | efficient splicing of musvts110 rna is restricted to temperatures of 33 degrees or lower. previously, we have shown that this conditional splicing event is mediated, in part, by cis-acting intronic sequences. we have now examined the role of exon sequences in musvts110 rna splicing. we found that deletion of all but 36 nucleotides of the gag exon (e1) yielded a transcript incapable of supporting splicing. however, inefficient, growth temperature-dependent splicing was recovered after restoration ... | 1992 | 1448919 |
| memory of butyrate induction by the moloney murine sarcoma virus enhancer-promoter element. | we reported previously that a housekeeping gene could be converted to a butyrate-inducible gene by replacing its cognate promoter with the moloney murine sarcoma virus enhancer-promoter element. in this study we report that the activated transcriptional state could be propagated from mother to daughter cells after the withdrawal of the inducer. | 1992 | 1449468 |
| substrate preference of metalloproteinases secreted by ts-110 moloney murine sarcoma virus-transformed normal rat kidney cells. | in this study, the two forms of affinity-purified transformation-associated proteins (taps) (68 and 64 kd) were shown to have different substrate preferences. for the 68-kd tap, the order of substrate preference was collagen types i, iii, and v; fibronectin; gelatin; and collagen iv. for the 64-kd tap, the order of substrate preference was collagen i, iii, and v and gelatin. the 64-kd tap did not cleave collagen iv and fibronectin. we also found a 71-kd metalloproteinase in the concentrated puri ... | 1992 | 1449476 |
| characterization of activated and normal mouse mos gene in murine 3t3 cells. | we have characterized the mouse mos proto-oncogene product, pp39mos, in murine fibroblasts. when expressed in nih3t3 cells under the influence of the long terminal repeat regulatory element from moloney murine sarcoma virus [nih(pts-1) cells], the mos protein was present in low levels and had a half-life of about 30 min. in extracts from nih(pts-1) cells, we detected additional forms of mos protein that apparently arose from internal initiation codons (p24mos and p29mos) or from upstream non-aug ... | 1992 | 1461652 |
| [adducts of 3'-azido-2,3'-dideoxythymidine 5'-phosphate or 5'-phosphonate as inhibitors of cytopathic effect and transformation of cells under the influence of retroviruses in cell culture]. | inhibition of hiv-1- or hiv-2-induced cytopathicity and (moloney) murine sarcoma virus (msv)-induced cell transformation by amino acid and amino alcohol adducts of either 3'-azido-2',3'-dideoxythymidine 5'-monophosphate (aztmp) or 5'-hydrogenphosphonate (azthp) were investigated. both types of nucleotide adducts inhibited replication of hiv-1 and hiv-2 in mt-4 cells at a 1.5- to 3-fold higher ec50 (50% effective concentration) than azt; and, also, selectivity indexes of these adducts were approx ... | 1992 | 1470177 |
| moloney murine sarcoma virus musvts110 dna: cloning, nucleotide sequence, and gene expression. | we have cloned moloney murine sarcoma virus (musv) musvts110 dna by assembly of polymerase chain reaction (pcr)-amplified segments of integrated viral dna from infected nrk cells (6m2 cells) and determined its complete sequence. previously, by direct sequencing of musvts110 rna transcribed in 6m2 cells, we established that the thermosensitive rna splicing phenotype uniquely characteristic of musvts110 results from a deletion of 1,487 nucleotides of progenitor musv-124 sequences. as anticipated, ... | 1992 | 1501276 |
| [2',5'-bis-o-(tert-butyldimethylsilyl)]-3'-spiro-5''-(4''-amino-1'',2''-oxathiole-2'',2''-dioxide) (tsao) derivatives of purine and pyrimidinenucleosides as potent and selective inhibitors of human immunodeficiency virus type 1. | the [2',5'-bis-o-(tert-butyldimethylsilyl)]-3'-spiro-5''-(4''-amino- 1'',2''-oxathiole-2'',2''-dioxide) (tsao) derivatives of ribofuranosylthymine, uridine, 5-bromouridine, 5-methylcytidine, inosine, and adenosine are potent and selective inhibitors of human immunodeficiency virus type 1 (hiv-1) but not of other retroviruses (hiv-2, simian immunodeficiency virus, or moloney murine sarcoma virus). the 50% effective concentration (ec50) of the most active tsao congeners for inhibition of hiv-1 rep ... | 1992 | 1510396 |
| suppression of transformed phenotype in hybrids of v-fgr and v-raf transformed rat-1 cells with rat embryonic fibroblasts is due to transcriptional inactivation of viral oncogenes. | rat-1 cells that had been transformed to tumorigenicity by transfection with the retroviral oncogenes v-raf from 3611-murine sarcoma virus, or v-fgr from gardner-rasheed feline sarcoma virus were fused with rat embryonic fibroblasts at an early passage. in both fusion experiments hybrid cells were isolated that exhibited normal morphology, anchorage requirement for proliferation, and either no tumorigenicity (v-fgr) or extended latency periods for tumor growth (v-raf) in nude mice. transcription ... | 1992 | 1563491 |
| expression of a 91-kilodalton pea3-binding protein is down-regulated during differentiation of f9 embryonal carcinoma cells. | proteins binding to the pea3 enhancer motif (aggaag) activate the polyomavirus early promoter and help comprise the viral late mrna initiator element (w. yoo, m. e. martin, and w. r. folk, j. virol. 65:5391-5400, 1991). because many developmentally regulated cellular genes have pea3 motifs near their promoter sequences, and because ets family gene products activate the pea3 motif, we have studied the expression of pea3-binding proteins and ets-related proteins during differentiation of f9 embryo ... | 1992 | 1569949 |
| human smooth muscle myosin light chain-2 gene expression is repressed in ras transformed fibroblast cells. | we have previously characterized human smooth muscle myosin light chain (mlc)-2 isoform by complementary dna cloning and have shown that this isoform is expressed in a number of nonmuscle cells such as fibroblast cells. in this report, we show that when human osteosarcoma derived clonal cells (te 85 clone f-5) (hos), which are immortalized and nontumorigenic, undergo transformation following infection by kirsten murine sarcoma virus (k-hos) or by a chemical carcinogen [n-methyl-n-nitro-n-nitroso ... | 1992 | 1599878 |
| overproduction of metalloproteinases by v-mos-transformed rat kidney (6m2) cells. | using monoclonal antibodies, we previously detected two forms of transformation-associated proteins (taps), p64 and p68, in the rat kidney (6m2) cells transformed by the temperature-sensitive 110-murine sarcoma virus-moloney-mutant. taps were secreted as glycoproteins by 6m2 cells grown at 33 degrees c, but not by 6m2 cells grown at 39 degrees c. the identity and functions of taps were previously unknown. by molecular cloning techniques and immunoscreening, we have isolated two different cdna cl ... | 1992 | 1602817 |
| sodium butyrate selectively induces transcription of promoters adjacent to the momsv viral enhancer. | the long terminal repeat region of the moloney murine sarcoma virus (momsv) was cloned upstream from the chinese hamster ovary adenine phosphoribosyltransferase (aprt)-encoding gene (aprt) in order to enhance synthesis of the aprt protein. the replacement of the native promoter with the viral enhancer-promoter increased the enzymatic activity of aprt two- to threefold. addition of sodium butyrate (nabu) to the cell growth medium induced aprt activity ten- to 20-fold above wild-type levels in bot ... | 1992 | 1634113 |
| eye pathology in transgenic mice carrying a msv-sv 40 large t-construct. | several lines of transgenic mice carrying a transgene construct consisting of the regulatory enhancer element of the moloney murine sarcoma virus and the simian virus 40 genome coding for the sv 40 promoter and the large t antigen were established. we describe several abnormalities found in the eyes of transgenic animals of which heritable cataract formation, probably due to disturbances in primary lens fibre differentiation, showed a close correlation to large t antigen expression. additionally ... | 1991 | 1651251 |
| transcriptional activation through ets domain binding sites in the cytochrome c oxidase subunit iv gene. | a mutational analysis of the rat cytochrome c oxidase subunit iv (rco4) promoter region revealed the presence of a major control element consisting of a tandemly repeated pair of binding sites for a nuclear factor from hela cells. this factor was designated nrf-2 (nuclear respiratory factor 2) because a functional recognition site was also found in the human atp synthase beta-subunit gene. deletion or site-directed point mutations of the nrf-2 binding sites in the rco4 promoter resulted in subst ... | 1991 | 1656236 |
| long-term explant culture of human colon and a 3-step transformation model for rat colonic epithelium. | our previous studies have suggested that colonic epithelium from rodents pretreated in vivo with suboptimal doses of carcinogen could be more easily maintained in explant culture. transformation of colonic epithelium from these explants may be induced by subsequent exposure to additional genotropic agents. therefore, we describe the development of a 3-step transformation model which uses (1) in vivo pretreatment with a suboptimal dose of 1,2-dimethylhydrazine (dmh) followed by (2) in vitro organ ... | 1991 | 1657027 |
| sv7, a molecular clone of moloney murine sarcoma virus 349, transforms vascular endothelial cells. | sv7, a progeny of moloney murine sarcoma virus 349 cells, was molecularly cloned. sv7 induced sarcomas consisting of vascular and fibrous components. the large blood-filled vascular dilatations appeared grossly as dark red spots in the tumors and constituted up to 50% of the tumor volume. these vascular structures, ranging from small capillaries to cavernous vascular dilatations, were lined by one to several layers of neoplastic endothelial cells. thick papillary outgrowths of the neoplastic end ... | 1991 | 1661076 |
| thy-1 as a negative growth regulator in ras-transformed mouse fibroblasts. | to identify molecules on the cell surface involved in negative growth regulation, we assumed that their amounts would be reduced after malignant transformation. we analyzed several proteins by fluorescence-activated cell sorter in mouse nih 3t3 and its transformed cell lines. surprisingly, the amount of thy-1, a cell surface glycoprotein anchored in the cell membrane by a glycophosphatidyl inositol linkage, was significantly decreased in the transformed nih 3t3 lines, especially in ras-transform ... | 1991 | 1670996 |
| retroviral recombination during reverse transcription. | after mixed infection, up to half of related retroviruses are recombinants. during infection, retroviral rna genomes are first converted to complementary dna (cdna) and then to double-stranded dna. thus recombination could occur during reverse transcription, by rna template switching, or after reverse transcription, by breakage and reunion of dna. it has not been possible to distinguish between these two potential mechanisms of recombination because both single-stranded cdna and double-stranded ... | 1990 | 1690424 |
| alkalinization of the lysosomes is correlated with ras transformation of murine and human fibroblasts. | the ph of the intralysosomal compartment of fibroblasts in culture was monitored by measuring the fluorescence emission intensity at 530 nm of fluid phase pinocytosed fluorescein-conjugated dextrans (fitc-dextrans) excited at 488 and 457 nm. following the procedure of ohkuma and poole (ohkuma, s., and poole, b. (1978) proc. natl. acad. sci. u. s. a. (1978) 75, 3327-3331), a relationship was established between the fluorescence emission intensity of the fitc-dextrans and ph. this correlation was ... | 1990 | 1690732 |
| involvement of interferon-system in the regulation of cell growth and differentiation. | in this report we review the current knowledge on the involvement of the interferon (ifn) system in the regulation of cell growth and differentiation. we also summarize our own data which provide evidence for the strong correlation between ifn-mediated growth-arrest of transformed cells and the elevated enzymatic activity of an ifn-induced protein. similarly, it is demonstrated that elevated levels of ifn-induced proteins accompany the early phases of in-vitro cell differentiation. ifn-treatment ... | 1990 | 1698310 |
| over-expression of the epidermal growth factor receptor in human breast cancer cells fails to induce an estrogen-independent phenotype. | an association exists in primary human breast tumors between high epidermal growth factor receptor (egfr) expression and a reduced number or even absence of estrogen receptors (er). to determine whether an increase in egfr expression might alter the estrogen responsiveness of an er-positive human breast cancer cell line, zr 75-1 cells were cotransfected with a plasmid containing the full-length cdna for the human egfr under the transcriptional control of the harvey murine sarcoma virus (hamsv) l ... | 1990 | 1698733 |
| normal human chromosome 1 carries suppressor activity for various phenotypes of a kirsten murine sarcoma virus-transformed nih/3t3 cell line. | in order to identify chromosomes that carry putative tumor-suppressor genes for the various phenotypes of kirsten sarcoma virus-transformed nih/3t3 (dt) cells, we performed microcell-mediated chromosome transfer into dt cells. we first isolated mouse a9 clones, containing a single human chromosome 1, 11 or 12 tagged with psv2-neo plasmid dna. then, chromosome 1, 11 or 12 was transferred from the a9 clones into dt cells by microcell fusion. the growth rate, colony-forming ability in soft agar and ... | 1990 | 1702413 |
| regulation of collagen gene expression by transformed human fibroblasts: decreased type i and type iii collagen rna transcription. | the regulation of collagen gene expression in normal diploid human fetal fibroblasts (kms-6 cells), and fibroblasts immortally transformed by treatment of kms-6 with co-60 gamma rays (kmst-6 cells) was compared to that of ones tumorigenically transformed by treatment of kmst-6 cells with harvey murine sarcoma virus (kmst-6-ras cells). synthesized collagenous protein decreased to approximately 30% of that of normal fetal fibroblasts in both transformed cell lines, and the relative rate of collage ... | 1991 | 1706744 |
| 9-[(2rs)-3-fluoro-2-phosphonylmethoxypropyl] derivatives of purines: a class of highly selective antiretroviral agents in vitro and in vivo. | a new class of compounds, 9-[(2rs)-3-fluoro-2-phosphonylmethoxypropyl] [(rs)-fpmp] derivatives of purines, is described that has selective activity against a broad spectrum of retroviruses [including human immunodeficiency virus type 1 (hiv-1) and type 2 (hiv-2)] but not other rna or dna viruses. this activity spectrum is completely different from that of the parental compounds, 9-[(2s)-3-hydroxy-2-phosphonylmethoxypropyl] [(s)-hpmp] derivatives of purines, which are active against a broad range ... | 1991 | 1711214 |
| tumours induced by moloney murine sarcoma virus are clonal in rats, not clonal in mice. | moloney murine sarcoma virus (m-msv) induces rapidly growing tumours in adult mice of most conventional strains. rats are less susceptible to m-msv oncogenesis, but the few rhabdomyosarcomas that do develop after viral inoculation of newborn animals closely resemble conventional malignancies: they develop after a long latency, grow progressively, and metastasize to regional lymph nodes and lungs. southern blot analysis with a v-mos-specific probe of m-msv-induced tumours in both species demonstr ... | 1992 | 1735735 |
| a divergent ets-related protein, elk-1, recognizes similar c-ets-1 proto-oncogene target sequences and acts as a transcriptional activator. | the ets oncogene superfamily consists of a family of sequence-specific dna-binding proteins that activate transcription. we have previously identified two new members of the ets oncogene superfamily, namely elk-1 and elk-2. in this report we show that the recombinant elk-1 protein expressed in bacteria, like the c-ets-1 proto-oncogene, binds in a sequence-specific manner to moloney murine sarcoma virus long terminal repeat, e74 target sequences and the pea3 motif (polyoma enhancer), but does not ... | 1992 | 1741166 |