Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| identification of domains of the tomato spotted wilt virus nsm protein involved in tubule formation, movement and symptomatology. | deletion and alanine-substitution mutants of the tomato spotted wilt virus nsm protein were generated to identify domains involved in tubule formation, movement and symptomatology using a heterologous tobacco mosaic virus expression system. two regions of nsm, g(19)-s(159) and g(209)-v(283), were required for both tubule formation in protoplasts and cell-to-cell movement in plants, indicating a correlation between these activities. three amino acid groups, d(154), eykk(205-208) and eeeee(284-288 ... | 2009 | 19481775 |
| analysis of gene expression profiles in leaf tissues of cultivated peanuts and development of est-ssr markers and gene discovery. | peanut is vulnerable to a range of foliar diseases such as spotted wilt caused by tomato spotted wilt virus (tswv), early (cercospora arachidicola) and late (cercosporidium personatum) leaf spots, southern stem rot (sclerotium rolfsii), and sclerotinia blight (sclerotinia minor). in this study, we report the generation of 17,376 peanut expressed sequence tags (ests) from leaf tissues of a peanut cultivar (tifrunner, resistant to tswv and leaf spots) and a breeding line (gt-c20, susceptible to ts ... | 2009 | 19584933 |
| a single tube, quantitative real-time rt-pcr assay that detects four potato viruses simultaneously. | a high throughput, real-time multiplex, single tube rt-pcr assay was developed for simultaneous detection of potato leafroll virus (plrv), potato virus x (pvx) and potato virus s (pvs) in potato leaves and tubers, and tomato spotted wilt virus (tswv) in potato tubers and tomato leaves. the test uses four different fluorescently labelled taqman probes. limits of detection sensitivity were established using a range of virus transcript copy numbers (8 x 10(1) to 8 x 10(9) copies of pvx and pvs, 1 x ... | 2009 | 19596379 |
| a generic method to identify plant viruses by high-resolution tandem mass spectrometry of their coat proteins. | although a number of protocols have been developed for detection of viruses at the genus or family level, universal approaches to detect and identify unknown viruses are still required. high-resolution tandem mass spectrometry was used to identify accurately peptide masses and their constituent sequences from partially purified plant virus preparations. analysis of the peptide fragment masses against a virus database using pattern-matching algorithms identified sequences with homology to known v ... | 2010 | 19712699 |
| comparative analysis of expression profiles in shoots and roots of tomato systemically infected by tomato spotted wilt virus reveals organ-specific transcriptional responses. | tomato (solanum lycopersicon), a model species for the family solanaceae, is severely affected by tomato spotted wilt virus (tswv) worldwide. to elucidate the systemic transcriptional response of plants to tswv infection, microarray experiments were performed on tomato. parallel analysis of both shoots and roots revealed organ-specific responses, although the virus was present in similar concentration. in the shoots, genes related to defense and to signal transduction were induced, while there w ... | 2009 | 19888816 |
| potatovirus x and tobacco mosaic virus-based vectors compatible with the gateway cloning system. | virus-based expression vectors are important tools for high-level production of foreign proteins and for gene function analysis through virus induced gene silencing. to exploit further their advantages as fast, high yield replicons, a set of vectors was produced by converting and adapting potato virus x (pvx) and tobacco mosaic virus (tmv)-based vectors to allow easy cloning of foreign sequences by the gateway cloning system. target genes were cloned efficiently by recombination and successfully ... | 2010 | 19903495 |
| a distinct tospovirus causing necrotic streak on alstroemeria sp. in colombia. | a tospovirus causing necrotic streaks on leaves was isolated from alstroemeria sp. in colombia. infected samples reacted positively with tomato spotted wilt virus (tswv) antiserum during preliminary serological tests. further analysis revealed a close serological relationship to tomato chlorotic spot virus (tcsv) and groundnut ringspot virus (grsv). a major part of the s-rna segment, encompassing the nucleocapsid (n) protein gene, the 5' untranslated region and a part of the intergenic region 3' ... | 2010 | 20151164 |
| comparative post-harvest behaviour of traditional and virus-resistant muchamiel tomatoes. | nowadays, organoleptic quality is the primary objective for almost all tomato breeding programmes. in this study, post-harvest behaviour of a breeding line with genetic resistance to important viruses (tomato mosaic virus, tomato spotted wilt virus and tomato yellow leaf curl virus) has been compared with the original traditional landrace (muchamiel). the breeding line has been obtained by backcrossing, introgressing three resistance genes but aiming to keep the quality characteristics of the tr ... | 2010 | 20355147 |
| tomato chocolate spot virus, a member of a new torradovirus species that causes a necrosis-associated disease of tomato in guatemala. | tomatoes in guatemala have been affected by a new disease, locally known as "mancha de chocolate" (chocolate spot). the disease is characterized by distinct necrotic spots on leaves, stems and petioles that eventually expand and cause a dieback of apical tissues. samples from symptomatic plants tested negative for infection by tomato spotted wilt virus, tobacco streak virus, tobacco etch virus and other known tomato-infecting viruses. a virus-like agent was sap-transmitted from diseased tissue t ... | 2010 | 20376682 |
| oligonucleotide microarray-based detection and identification of 10 major tomato viruses. | a dna microarray chip was developed for screening 10 major economically important tomato viruses from infected plants using "combimatrix" platform 40-mer oligonucleotide probes. a total of 279 oligonucleotide virus probes were specific for simultaneous multiple detection, identification, differentiation and/or genotyping of each of the following tomato rna viruses and/or strains and a virus satellite: cucumber mosaic virus, cucumber mosaic virus satellite rna, tomatoinfectiouschlorosisvirus, tom ... | 2010 | 20470828 |
| analysis of expressed sequence tags for frankliniella occidentalis, the western flower thrips. | thrips are members of the insect order thysanoptera and frankliniella occidentalis (the western flower thrips) is the most economically important pest within this order. f. occidentalis is both a direct pest of crops and an efficient vector of plant viruses, including tomato spotted wilt virus (tswv). despite the world-wide importance of thrips in agriculture, there is little knowledge of the f. occidentalis genome or gene functions at this time. a normalized cdna library was constructed from fi ... | 2010 | 20522119 |
| resistance to a dna and a rna virus in transgenic plants by using a single chimeric transgene construct. | tomato leaf curl taiwan virus (tolctwv) and tomato spotted wilt virus (tswv) are two major tomato viruses that cause serious economic losses. in this study, a partial c2 gene from tolctwv and the middle half of the n gene of tswv were fused as a chimeric transgene to develop multiple virus resistance in transgenic plants. this construct was introduced into nicotiana benthamiana and tomato by agrobacterium-mediated transformation. several transgenic lines showed no symptom post agro-inoculation w ... | 2011 | 20559871 |
| expression of a modified nucleocapsid-protein of tomato spotted wilt virus (tswv) confers resistance against tswv and groundnut ringspot virus (grsv) by blocking systemic spread. | summary tobacco transformed with a construct comprising the nucleocapsid-protein (n) gene of tomato spotted wilt virus (tswv) and the 5' non-translated leader sequence of plum pox virus (ppv) as a translation enhancer displays unusually broad resistance against tospoviruses. contrary to expectations, the transgenic plants did not express a higher level of tswv n protein than control plants harbouring the n gene without the ppv enhancer. instead, expression of an n-terminally elongated n protein, ... | 2004 | 20565598 |
| tomato spotted wilt virus-positive steps towards negative success. | abstract taxonomy: tomato spotted wilt virus (tswv) is the type member of the plant-infecting tospovirus genus in the family bunyaviridae, a large group of predominantly vertebrate- and insect-infecting rna viruses. physical properties: virions are 80-120-nm pleomorphic particles with surface projections composed of two viral glycoproteins, g1 and g2 (fig. 1). virion composition is 5% nucleic acid, 70% protein, 5% carbohydrate and 20% lipid. the genome consists of three negative or ambisense ssr ... | 2000 | 20572961 |
| diverging affinity of tospovirus rna silencing suppressor proteins, nss, for various rna duplex molecules. | the tospovirus nss protein was previously shown to suppress the antiviral rna silencing mechanism in plants. here the biochemical analysis of nss proteins from different tospoviruses, using purified nss or nss containing cell extracts, is described. the results showed that all tospoviral nss proteins analyzed exhibited affinity to small double-stranded rna molecules, i.e., small interfering rnas (sirnas) and micro-rna (mirna)/mirna* duplexes. interestingly, the nss proteins from tomato spotted w ... | 2010 | 20739523 |
| population genetic analysis of tomato spotted wilt virus on peanut in north carolina and virginia. | exploring the genetic diversity and evolutionary history of plant viruses is critical to understanding their ecology and epidemiology. in this study, maximum-likelihood and population genetics-based methods were used to investigate the population structure, genetic diversity, and sources of genetic variation in field isolates of tomato spotted wilt virus (tswv) from peanut in north carolina and virginia. selected regions of the nucleocapsid, movement, and rna-dependent rna polymerase genes were ... | 2011 | 20839960 |
| evolutionary analysis of tomato sw-5 resistance-breaking isolates of tomato spotted wilt virus. | tomato spotted wilt virus (tswv) causes severe economic losses in many crops worldwide and often overcomes resistant cultivars used for disease control. comparison of nucleotide and amino acid sequences suggested that tomato resistance conferred by the gene sw-5 can be overcome by the amino acid substitution c to y at position 118 (c118y) or t120n in the tswv movement protein, nsm. phylogenetic analysis revealed that substitution c118y has occurred independently three times in the studied isolat ... | 2010 | 20881087 |
| a silencing suppressor protein (nss) of a tospovirus enhances baculovirus replication in permissive and semipermissive insect cell lines. | the nonstructural protein (nss) of the tomato spotted wilt virus (tswv) has been identified as an rnai suppressor in plant cells. a recombinant autographa californica multiple nucleopolyhedrovirus (acmnpv) designated vacnss, containing the nss gene under the control of the viral polyhedrin (polh) gene promoter, was constructed and the effects of nss in permissive, semipermissive and nonpermissive insect cells to vacnss infection were evaluated. vacnss produced more budded virus when compared to ... | 2010 | 20971139 |
| thermal inactivation of tomato spotted wilt virus. | 1946 | 20985881 | |
| inactivation of tomato spotted wilt virus by salicylate. | 1946 | 20985882 | |
| identification and validation of reference genes for normalization of transcripts from virus-infected arabidopsis thaliana. | real-time quantitative polymerase chain reaction (qpcr) of complementary dna is now a standard method for studies of gene expression. however, qpcr can identify genuine variation only when transcript quantities are accurately normalized to an appropriate reference. to identify the most reliable reference genes for transcript quantification by qpcr, we describe a systematic evaluation of candidate reference genes of arabidopsis thaliana ecotype columbia-0 (col-0). twelve genes were selected for t ... | 2011 | 21091160 |
| explaining loss caused by tomato spotted wilt virus on tobacco with boreal winter weather: a bayesian approach. | in north carolina, tomato spotted wilt virus (tswv) has regularly been reported since 1997, with incidence being the highest in 2002. at the end of each season, a questionnaire is sent to the county agents to report disease losses. tswv reported losses in 1993 to 2007 from 58 counties were available. a county-year combination was considered a case and, in total, 494 cases were analyzed. the winter months' temperature and precipitation significantly explained the reported tswv loss (r(2) = 0.82). ... | 2011 | 21091184 |
| complete nucleotide sequence of capsicum chlorosis virus isolated from phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses. | phalaenopsis orchids are popular ornamentals all over the world. a tospovirus, capsicum chlorosis virus (cacv-ph) had been identified as the cause of chlorotic ringspots on leaves of phalaenopsis orchids in taiwan. the tripartite genome of cacv-ph was found to contain 3608, 4848 and 8916 nt of s, m and l rnas, respectively. phylogenetic analysis of the nucleocapsid (n) protein confirmed that cacv-ph is a member of the watermelon silver mottle virus (wsmov) serogroup in the genus tospovirus. base ... | 2010 | 21161553 |
| evolution and structure of tomato spotted wilt virus populations: evidence of extensive reassortment and insights into emergence processes. | tomato spotted wilt virus (tswv; genus tospovirus, family bunyaviridae) genetic diversity was evaluated by sequencing parts of the three rna genome segments of 224 isolates, mostly from pepper and tomato crops in southern europe. eighty-three per cent of the isolates showed consistent clustering into three clades, corresponding to their geographical origin, spain, france or the usa, for the three rna segments. in contrast, the remaining 17% of isolates did not belong to the same clade for the th ... | 2010 | 21169211 |
| complete genome sequences of three tomato spotted wilt virus isolates from tomato and pepper plants in korea and their phylogenetic relationship to other tswv isolates. | tomato spotted wilt virus (tswv) infects numerous host plants and has three genome segments, called l, m and s. here, we report the complete genome sequences of three korean tswv isolates (tswv-1 to -3) infecting tomato and pepper plants. although the nucleotide sequence of tswv-1 genome isolated from tomato is very different from those of tswv-2 and tswv-3 isolated from pepper, the deduced amino acid sequences of the five tswv genes are highly conserved among all three tswv isolates. in phyloge ... | 2011 | 21327785 |
| using small rna sequences to diagnose, sequence, and investigate the infectivity characteristics of vegetable-infecting viruses. | in a virus-infected plant, small interfering rnas (sirnas) corresponding to the viral genome form a large proportion of the small rna population. it is possible to reassemble significant portions of the virus sequence from overlapping sirna sequences and use these to identify the virus. we tested this technique with a resistance-breaking and a non-resistance-breaking strain of tomato spotted wilt virus (tswv). we were able to assemble contigs covering 99% of the genomes of both viruses. the abun ... | 2011 | 21448740 |
| evaluation of dna fragments covering the entire genome of a monopartite begomovirus for induction of viral resistance in transgenic plants via gene silencing. | tomato-infecting begomoviruses, a member of whitefly-transmitted geminivirus, cause the most devastating virus disease complex of cultivated tomato crops in the tropical and subtropical regions. numerous strategies have been used to engineer crops for their resistance to geminiviruses. however, nearly all have concentrated on engineering the replication-associated gene (rep), but not on a comprehensive evaluation of the entire virus genome. in this study, tomato leaf curl taiwan virus (tolctwv), ... | 2011 | 21597979 |
| infection with a plant virus modifies vector feeding behavior. | vector infection by some animal-infecting parasites results in altered feeding that enhances transmission. modification of vector behavior is of broad adaptive significance, as parasite fitness relies on passage to a new host, and vector feeding is nearly always essential for transmission. although several plant viruses infect their insect vectors, we have shown that vector infection by a plant virus alters feeding behavior. here we show that infection with tomato spotted wilt virus (tswv), type ... | 2011 | 21606372 |
| epidemiology of spotted wilt disease of peanut caused by tomato spotted wilt virus in the southeastern u.s. | spotted wilt disease of peanut (arachis hypogaea) (swp), caused by tomato spotted wilt virus (tswv) (genus tospovirus, family bunyaviridae), was first observed in alabama, florida, and georgia in the late 1980s and rapidly became a major limiting factor for peanut production in the region. tobacco thrips (frankliniella fusca) and western flower thrips (frankliniella occidentalis) both occur on peanut throughout the southeastern u.s., but f. fusca is the predominant species that reproduces on pea ... | 2011 | 21620508 |
| detection, discrimination and absolute quantitation of tomato spotted wilt virus isolates using real time rt-pcr with taqman(®)mgb probes. | a quantitative real-time reverse transcription-polymerase chain reaction (rt-qpcr) procedure using a general primer set and three taqman(®)mgb probes was developed for general and genotype-specific detection and quantitation of the genomic m segment of tomato spotted wilt virus (tswv). standard curves using rna transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) tswv m segment rna copies/ng of total rna) and high sensitivity ... | 2011 | 21635923 |
| complete genome sequence of a tomato spotted wilt virus isolate from china and comparison to other tswv isolates of different geographic origin. | tomato spotted wilt virus (tswv) is well established in most countries worldwide, while it is rarely reported in china. in this report, we have determined the complete nucleotide sequence of a tswv isolate named tswv-yn infecting tomato in yunnan province in southwestern china. the tripartite genome of tswv-yn was found to consist of l, m and s rnas of 8910, 4773 and 2970 nt, respectively. the complete genome sequence and the sequence of each genomic region of tswv-yn from china were compared to ... | 2011 | 21805095 |
| Recent advances in the molecular and cellular biology of bunyaviruses. | The family Bunyaviridae of segmented, negative-stranded RNA viruses includes over 350 members that infect a bewildering variety of animals and plants. Many of these bunyaviruses are the causative agents of serious disease in their respective hosts, and are classified as emerging viruses because of their increased incidence in new populations and geographical locations throughout the world. Emerging bunyaviruses, such as Crimean-Congo hemorrhagic fever virus, tomato spotted wilt virus and Rift Va ... | 2011 | 21865443 |
| arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation. | tomato (solanum lycopersicum) can establish symbiotic interactions with arbuscular mycorrhizal (am) fungi, and can be infected by several pathogenic viruses. here, we investigated the impact of mycorrhization by the fungus glomus mosseae on the tomato spotted wilt virus (tswv) infection of tomato plants by transcriptomic and hormones level analyses. in tswv-infected mycorrhizal plants, the am fungus root colonization limited virus-induced changes in gene expression in the aerial parts. the virus ... | 2011 | 21899386 |
| modified expression of alternative oxidase in transgenic tomato and petunia affects the level of tomato spotted wilt virus resistance. | abstract: | 2011 | 22014312 |
| top 10 plant viruses in molecular plant pathology. | many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. however, to our knowledge, no such list exists. the aim of this review was to survey all plant virologists with an association with molecular plant pathology and ask them to nominate which plant viruses they would place in a 'top 10' based on scientific/economic importance. the survey generated more than 250 votes from the international community, and allowed the gen ... | 2011 | 22017770 |
| The nucleocapsid protein of an enveloped plant virus, Tomato spotted wilt virus, facilitates long-distance movement of Tobacco mosaic virus hybrids. | To investigate the potential role(s) of the nucleocapsid (N) protein of Tomato spotted wilt virus (TSWV), the open reading frame for the N protein was expressed from a Tobacco mosaic virus (TMV)-based vector encoding only the TMV replicase proteins. In the absence of other TSWV-encoded proteins, the transiently expressed N protein facilitated long-distance movement of the TMV-based hybrids in transgenic Nicotiana benthamiana [NB-MP(+)] expressing movement protein of TMV, thus providing the funct ... | 2011 | 22020361 |
| an integrated genetic linkage map of cultivated peanut (arachis hypogaea l.) constructed from two ril populations. | construction and improvement of a genetic map for peanut (arachis hypogaea l.) continues to be an important task in order to facilitate quantitative trait locus (qtl) analysis and the development of tools for marker-assisted breeding. the objective of this study was to develop a comparative integrated map from two cultivated × cultivated recombinant inbred line (ril) mapping populations and to apply in mapping tomato spotted wilt virus (tswv) resistance trait in peanut. a total of 4,576 simple s ... | 2011 | 22072100 |
| Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. | In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most ... | 2011 | 22149499 |
| Antagonistic plant defense system regulated by phytohormones assists interactions among vector insect, thrips, and a tospovirus. | The western flower thrips (Frankliniella occidentalis) is a polyphagous herbivore that causes serious damage to many agricultural plants. In addition to causing feeding damage, it is also a vector insect that transmits tospoviruses such as Tomato spotted wilt virus (TSWV). We previously reported that thrips feeding on plants induces a jasmonate (JA)-regulated plant defense, which negatively affects both the performance and preference (i.e., host plant attractiveness) of the thrips. The antagonis ... | 2011 | 22180600 |
| sequence determination and analysis of the nss genes of two tospoviruses. | the tospoviruses groundnut ringspot virus (grsv) and zucchini lethal chlorosis virus (zlcv) cause severe losses in many crops, especially in solanaceous and cucurbit species. in this study, the non-structural nss gene and the 5'utrs of these two biologically distinct tospoviruses were cloned and sequenced. the nss sequence of grsv and zlcv were both 1,404 nucleotides long. pairwise comparison showed that the nss amino acid sequence of grsv shared 69.6% identity with that of zlcv and 75.9% identi ... | 2011 | 22187101 |
| analysis of the tomato spotted wilt virus ambisense s rna-encoded hairpin structure in translation. | the intergenic region (ir) of ambisense rna segments from animal- and plant-infecting (-)rna viruses functions as a bidirectional transcription terminator. the ir sequence of the tomato spotted wilt virus (tswv) ambisense s rna contains stretches that are highly rich in a-residues and u-residues and is predicted to fold into a stable hairpin structure. the presence of this hairpin structure sequence in the 3' untranslated region (utr) of tswv mrnas implies a possible role in translation. | 2012 | 22363535 |
| transmission of iris yellow spot virus by frankliniella fusca and thrips tabaci (thysanoptera: thripidae). | thrips-transmitted iris yellow spot virus (iysv) (family bunyaviridae, genus tospovirus) affects onion production in the united states and worldwide. the presence of iysv in georgia was confirmed in 2003. two important thrips species that transmit tospoviruses, the onion thrips (thrips tabaci (lindeman)) and the tobacco thrips (frankliniella fusca (hinds)) are known to infest onion in georgia. however, t. tabaci is the only confirmed vector of iysv. experiments were conducted to test the vector ... | 2012 | 22420253 |
| isolation and analysis of small rnas from virus-infected plants. | in this chapter, we detail some of the methods available to the researcher for isolating and analyzing virus-derived small rnas (vsrnas). these methods have been successfully used for four plant viruses: cucumber mosaic virus (cmv), including the cmv y-satellite, turnip mosaic virus (tumv), potato leaf roll virus (plrv), and tomato spotted wilt virus (tswv) from inoculated arabidopsis thaliana plants (fusaro et al. embo rep 7:1168-1175, 2006; curtin et al. febs lett 582:2753-2760, 2008). the pro ... | 2012 | 22678580 |
| major tomato viruses in the mediterranean basin. | tomato (solanum lycopersicum l.) originated in south america and was brought to europe by the spaniards in the sixteenth century following their colonization of mexico. from europe, tomato was introduced to north america in the eighteenth century. tomato plants show a wide climatic tolerance and are grown in both tropical and temperate regions around the world. the climatic conditions in the mediterranean basin favor tomato cultivation, where it is traditionally produced as an open-field plant. ... | 2012 | 22682165 |
| viruses of pepper crops in the mediterranean basin: a remarkable stasis. | compared to other vegetable crops, the major viral constraints affecting pepper crops in the mediterranean basin have been remarkably stable for the past 20 years. among these viruses, the most prevalent ones are the seed-transmitted tobamoviruses; the aphid-transmitted potato virus y and tobacco etch virus of the genus potyvirus, and cucumber mosaic virus member of the genus cucumovirus; and thrips-transmitted tospoviruses. the last major viral emergence concerns the tospovirus tomato spotted w ... | 2012 | 22682167 |
| virus diseases in lettuce in the mediterranean basin. | lettuce is frequently attacked by several viruses causing disease epidemics and considerable yield losses along the mediterranean basin. aphids are key pests and the major vectors of plant viruses in lettuce fields. lettuce mosaic virus (lmv) is probably the most important because it is seed-transmitted in addition to be transmissible by many aphid species that alight on the crop. tomato spotted wilt virus (tswv) is another virus that causes severe damage since the introduction of its major vect ... | 2012 | 22682170 |
| tospoviruses in the mediterranean area. | tospoviruses are among the most serious threats to vegetable crops in the mediterranean basin. tospovirus introduction, spread, and the diseases these viruses cause have been traced by epidemiological case studies. recent research has centered on the close relationship between tospoviruses and their arthropod vectors (species of the thripidae family). here, we review several specific features of tospovirus-thrips associations in the mediterranean. since the introduction of frankliniella occident ... | 2012 | 22682175 |
| proteomic analysis of frankliniella occidentalis and differentially expressed proteins in response to tomato spotted wilt virus infection. | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis in a persistent propagative manner. despite the extensive replication of tswv in midgut and salivary glands, there is little to no pathogenic effect on f. occidentalis. we hypothesize that the first-instar larva (l1) of f. occidentalis mounts a response to tswv that protects it from pathogenic effects caused by virus infection and replication in various insect tissues. a partial thrips transcriptome was generated using ... | 2012 | 22696645 |
| effectiveness of tomato-spotted wilt virus management tactics. | tomato-spotted wilt (tsw) is caused by the tomato-spotted wilt virus (tswv) and is a major disease affecting the production of tomato and pepper in the southeastern united states. before initiating a multistate, regional project addressing this issue, a survey was conducted in north carolina, south carolina, georgia, and florida to assess the need for improved management of tswv in these crops. we investigated farmer's stated effectiveness of four common tswv management tactics (reflective mulch ... | 2012 | 22812134 |
| reflective mulch and acibenzolar-s-methyl treatments relative to thrips (thysanoptera: thripidae) and tomato spotted wilt virus incidence in tomato. | management of thrips-transmitted tomato spotted wilt (tsw) virus typically relies on tactics that either reduce the thrips vector numbers or change the plant's response to the virus to reduce economic loss. we attempted to quantify the interaction between two such tactics, reflective mulch and the plant activator acibenzolar-s-methyl (actigard), respectively, on a tsw-susceptible tomato hybrid. a split plot experiment was conducted in 2009 and 2010 where main-plots were three types of plastic mu ... | 2012 | 22928310 |
| simultaneous detection of the seven main tomato-infecting rna viruses by two multiplex reverse transcription polymerase chain reactions. | cucumber mosaic virus, tomato spotted wilt virus, tomato mosaic virus, tomato chlorosis virus, pepino mosaic virus, torrado tomato virus and tomato infectious chlorosis virus cause serious damage and significant economic losses in tomato crops worldwide. the early detection of these pathogens is essential for preventing the viruses from spreading and improving their control. in this study, a procedure based on two multiplex rt-pcrs was developed for the sensitive and reliable detection of these ... | 2012 | 22939978 |
| complementation between two tospoviruses facilitates the systemic movement of a plant virus silencing suppressor in an otherwise restrictive host. | new viruses pathogenic to plants continue to emerge due to mutation, recombination, or reassortment among genomic segments among individual viruses. tospoviruses cause significant economic damage to a wide range of crops in many parts of the world. the genetic or molecular basis of the continued emergence of new tospoviruses and new hosts is not well understood though it is generally accepted that reassortment and/or genetic complementation among the three genomic segments of individual viruses ... | 2012 | 23077485 |
| effectiveness of combining resistance to thielaviopsis basicola and tomato spotted wilt virus in haploid tobacco genotypes. | black root rot (brr) caused by thielaviopsis basicola as well as tomato spotted wilt virus (tswv) are the most serious problems in tobacco growing regions. we crossed the breeding line wgl 3 carrying brr resistance derived from n.glauca with the line pw-834 the resistance of which to tswv was transferred from cultivar polalta. anthers obtained from f(1) hybrid plants were cultured to induce haploids combining resistance to th. basicola and tswv. flow cytometry analysis revealed 242 haploids and ... | 2011 | 23136476 |
| [expression and subcellular location of nsm protein of tomato spotted wilt virus in plant and insect cells]. | expression and subcellular location of nsm protein of tomato spotted wilt virus were studied using plant and insect cells. | 2012 | 23173432 |
| specific insect-virus interactions are responsible for variation in competency of different thrips tabaci isolines to transmit different tomato spotted wilt virus isolates. | local adaptation between sympatric host and parasite populations driven by vector genetics appears to be a factor that influences dynamics of disease epidemics and evolution of insect-vectored viruses. although t. tabaci is the primary vector of tomato spotted wilt virus (tswv) in some areas of the world, it is not an important vector of this economically important plant virus in many areas where it occurs. previous studies suggest that genetic variation of thrips populations, virus isolates, or ... | 2013 | 23358707 |
| tsw gene-based resistance is triggered by a functional rna silencing suppressor protein of the tomato spotted wilt virus. | as a result of contradictory reports, the avirulence (avr) determinant that triggers tsw gene-based resistance in capsicum annuum against the tomato spotted wilt virus (tswv) is still unresolved. here, the n and nss genes of resistance-inducing (ri) and resistance-breaking (rb) isolates were cloned and transiently expressed in resistant capsicum plants to determine the identity of the avr protein. it was shown that the nss(ri) protein triggered a hypersensitive response (hr) in tsw-containing ca ... | 2013 | 23360130 |
| thrips tabaci population genetic structure and polyploidy in relation to competency as a vector of tomato spotted wilt virus. | knowledge of population-level genetic differences can help explain variation among populations of insect vectors in their role in the epidemiology of specific viruses. variation in competency to transmit tomato spotted wilt virus (tswv) that exists among populations of thrips tabaci has been associated with the presence of cryptic species that exhibit different modes of reproduction and host ranges. however, recent findings suggest that vector competency of t. tabaci at any given location depend ... | 2013 | 23365671 |
| identification of expressed resistance gene analogs from peanut (arachis hypogaea l.) expressed sequence tags. | low genetic diversity makes peanut (arachis hypogaea l.) very vulnerable to plant pathogens, causing severe yield loss and reduced seed quality. several hundred partial genomic dna sequences as nucleotide-binding-site leucine-rich repeat (nbs-lrr) resistance genes (r) have been identified, but a small portion with expressed transcripts has been found. we aimed to identify resistance gene analogs (rgas) from peanut expressed sequence tags (ests) and to develop polymorphic markers. the protein seq ... | 2013 | 23384141 |
| de novo transcriptome sequencing in frankliniella occidentalis to identify genes involved in plant virus transmission and insecticide resistance. | the western flower thrips (wft), frankliniella occidentalis, a world-wide invasive insect, causes agricultural damage by directly feeding and by indirectly vectoring tospoviruses, such as tomato spotted wilt virus (tswv). we characterized the transcriptome of wft and analyzed global gene expression of wft response to tswv infection using illumina sequencing platform. we compiled 59,932 unigenes, and identified 36,339 unigenes by similarity analysis against public databases, most of which were an ... | 2013 | 23434629 |
| development of a real-time fluorescent quantitative pcr assay for detection of impatiens necrotic spot virus. | impatiens necrotic spot virus (insv) is an important plant virus that can cause severe disease in various ornamental and agricultural crops. several species of thrips transmit insv, of which the western flower thrip (frankliniella occidentalis) is the most important. in this study, primers and taqman probes based on insv non-structural protein gene sequences were designed, and a technique was developed for detecting insv using fluorescent quantitative rt-pcr. the reproducibility, specificity and ... | 2013 | 23466630 |
| oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato virus x-potato virus y and tomato spotted wilt virus. | one of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis ... | 2013 | 23487466 |
| genetic and host-associated differentiation within thrips tabaci lindeman (thysanoptera: thripidae) and its links to tomato spotted wilt virus-vector competence. | of eight thelytokous populations of onion thrips (thrips tabaci) collected from potato (three populations), onion (four) or chrysanthemum (one) hosts from various regions of australia, only those from potato were capable of transmitting tomato spotted wilt virus (tswv) in controlled transmission experiments. genetic differentiation of seven of these eight populations, and nine others not tested for tswv vector competence, was examined by comparison of the dna sequences of mitochondrial cytochrom ... | 2013 | 23632893 |
| phylogenetic and recombination analysis of tomato spotted wilt virus. | tomato spotted wilt virus (tswv) severely damages and reduces the yield of many economically important plants worldwide. in this study, we determined the whole-genome sequences of 10 tswv isolates recently identified from various regions and hosts in korea. phylogenetic analysis of these 10 isolates as well as the three previously sequenced isolates indicated that the 13 korean tswv isolates could be divided into two groups reflecting either two different origins or divergences of korean tswv is ... | 2013 | 23696821 |
| expression, purification, crystallization and preliminary x-ray crystallographic study of the nucleocapsid protein of tomato spotted wilt virus. | tomato spotted wilt virus (tswv), which causes severe damage to various agricultural crops such as tomato, pepper, lettuce and peanut, is a negative-stranded rna virus belonging to the tospovirus genus of the bunyaviridae family. viral genomic rna molecules are packaged in the form of ribonucleoprotein complexes, each of which contains one viral rna molecule that is coated with many nucleocapsid (n) proteins. here, the expression and crystallization of tswv n protein are reported. native and sel ... | 2013 | 23722858 |
| from immunity to susceptibility: virus resistance induced in tomato by a silenced transgene is lost as tgs overcomes ptgs. | tomato line 30.4 was obtained engineering the nucleocapsid (n) gene of tomato spotted wilt virus into plant genome, and immunity to tomato spotted wilt virus infection of its self-pollinated homozygous progeny was observed. despite the presence of a high amount of transgenic transcripts, transgenic proteins have not been detected, suggesting a mechanism of resistance mediated by rna. in the present study, we identify post-transcriptional gene silencing as the main mechanism of resistance, which ... | 2013 | 23738576 |
| second generation peanut genotypes resistant to thrips-transmitted tomato spotted wilt virus exhibit tolerance rather than true resistance and differentially affect thrips fitness. | spotted wilt disease caused by tomato spotted wilt virus (tswv) (family bunyaviridae; genus tospovirus) is a major constraint to peanut (arachis hypogaea l.) production in the southeastern united states. reducing yield losses to tswv has heavily relied on planting genotypes that reduce the incidence of spotted wilt disease. however, mechanisms conferring resistance to tswv have not been identified in these genotypes. furthermore, no information is available on how these genotypes influence thrip ... | 2013 | 23786043 |
| tomato spotted wilt virus infection reduces the fitness of a nonvector herbivore on pepper. | plant pathogens and insect herbivores often share hosts under natural conditions. hence, pathogen-induced changes in a host plant can affect the herbivore and vice versa. even though plant viruses are ubiquitous in the field, little is known about plant-mediated interactions between viruses and nonvector herbivores. here we tested whether the performance of the sweet potato whitefly, bemisia tabaci (gennadius) biotype q, was altered when raised on pepper infected with tomato spotted wilt virus ( ... | 2013 | 23786083 |
| development of apple latent spherical virus-based vaccines against three tospoviruses. | apple latent spherical virus (alsv) is characterized by its relatively broad host range, latency in most host plants, and ability to induce gene silencing in host plants. herein, we focus on the above characteristic of alsv and describe our development of alsv vector vaccines against three tospoviruses, namely, impatiens necrotic spot virus (insv), iris yellow spot virus (iysv), and tomato spotted wilt virus (tswv). dna fragments of 201 nt of three tospovirus s-rnas (silencing suppressor (nss) a ... | 2013 | 23850843 |
| interacting virus abundance and transmission intensity underlie tomato spotted wilt virus incidence: an example weather-based model for cultivated tobacco. | through a modeling approach, we investigated weather factors that affect the summer incidence of tomato spotted wilt virus (tswv), a virus vectored exclusively by thrips, in cultivated tobacco. aspects of thrips and plant biology that affect disease spread were treated as functions of weather, leading to a model of disease incidence informed by thrips and plant biology, and dependent on weather input variables. we found that disease incidence during the summer was influenced by weather affecting ... | 2013 | 23977384 |
| host plant resistance against tomato spotted wilt virus in peanut (arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival. | tomato spotted wilt virus (tswv) severely affects peanut production in the southeastern united states. breeding efforts over the last three decades resulted in the release of numerous peanut genotypes with field resistance to tswv. the degree of field resistance in these genotypes has steadily increased over time, with recently released genotypes exhibiting a higher degree of field resistance than older genotypes. however, most new genotypes have never been evaluated in the greenhouse or laborat ... | 2014 | 24025049 |
| tomato spotted wilt virus benefits a non-vector arthropod, tetranychus urticae, by modulating different plant responses in tomato. | the interaction between plant viruses and non-vector arthropod herbivores is poorly understood. however, there is accumulating evidence that plant viruses can impact fitness of non-vector herbivores. in this study, we used oligonucleotide microarrays, phytohormone, and total free amino acid analyses to characterize the molecular mechanisms underlying the interaction between tomato spotted wilt virus (tswv) and a non-vector arthropod, twospotted spider mite (tetranychusurticae), on tomato plants, ... | 2013 | 24058708 |
| non-structural proteins of arthropod-borne bunyaviruses: roles and functions. | viruses within the bunyaviridae family are tri-segmented, negative-stranded rna viruses. the family includes several emerging and re-emerging viruses of humans, animals and plants, such as rift valley fever virus, crimean-congo hemorrhagic fever virus, la crosse virus, schmallenberg virus and tomato spotted wilt virus. many bunyaviruses are arthropod-borne, so-called arboviruses. depending on the genus, bunyaviruses encode, in addition to the rna-dependent rna polymerase and the different struct ... | 2013 | 24100888 |
| analysis of tomato spotted wilt virus nss protein indicates the importance of the n-terminal domain for avirulence and rna silencing suppression. | recently, tomato spotted wilt virus (tswv) nonstructural protein nss has been identified unambiguously as an avirulence (avr) determinant for tomato spotted wilt (tsw)-based resistance. the observation that nss from two natural resistance-breaking isolates had lost rna silencing suppressor (rss) activity and avr suggested a link between the two functions. to test this, a large set of nss mutants was generated by alanine substitutions in nss from resistance-inducing wild-type strains (nss(ri) ), ... | 2014 | 24103150 |
| differential expression of tomato spotted wilt virus-derived viral small rnas in infected commercial and experimental host plants. | viral small rnas (vsirnas) in the infected host can be generated from viral double-stranded rna replicative intermediates, self-complementary regions of the viral genome or from the action of host rna-dependent rna polymerases on viral templates. the vsirna abundance and profile as well as the endogenous small rna population can vary between different hosts infected by the same virus influencing viral pathogenicity and host response. there are no reports on the analysis of vsirnas of tomato spot ... | 2013 | 24143182 |
| identification and mapping on chromosome 9 of rapd markers linked to sw-5 in tomato by bulked segregant analysis. | bulked segregant analysis was used to identify random amplified polymorphic dna (rapd) markers linked to the sw-5 gene for resistance to tomato spotted wilt virus (tswv) in tomato. using two pools of phenotyped individuals from one segregating population, we identified four rapd markers linked to the gene of interest. two of these appeared tightly linked to sw-5, whereas another, linked in repulsion phase, enabled the identification of heterozygous and susceptible plants. after linkage analysis ... | 1996 | 24166634 |
| allelic relationships between genes for resistance to tomato spotted wilt tospovirus in capsicum chinense. | pepper (capsicum chinense jacq.) has been reported to be an important reservoir of resistance genes to tomato spotted wilt virus (tswv). the genes for tswv resistance present in three c. chinense lines ('pi 152225', 'pi 159236' and 'panca') were investigated for allelism. all resistant lines were crossed with each other. parents, f1, backcrosses and f2 populations (including reciprocals) developed from those crosses were mechanically inoculated with a highly virulent tswv isolate. susceptible c. ... | 1995 | 24173797 |
| mapping the sw-5 locus for tomato spotted wilt virus resistance in tomatoes using rapd and rflp analyses. | the sw-5 locus confers dominant resistance to tomato spotted wilt virus (tswv). to map the location and facilitate the identification of markers linked to sw-5 we developed a pair of near-isogenic lines (nils) and an f2 lycopersicon esculentum x l. pennellii population segregating for resistance to tswv. dna from the nils was analyzed using 748 random 10-mer oligonucleotides to discern linked molecular markers using a random amplified polymorphic dna (rapd) approach. one random primer (gagcacggg ... | 1995 | 24173937 |
| biolistic transformation of chrysanthemum with the nucleocapsid gene of tomato spotted wilt virus. | in vitro regeneration and biolistic transformation procedures were developed for several commercial chrysanthemum dendranthema grandiflora tzvelev, syn. chrysanthemum morifolium ramat. cultivars using leaf and stem explants. studies on the effect of several growth regulators and kanamycin on chrysanthemum regeneration were conducted, and a step-wise procedure to optimize kanamycin selection and recovery of transgenic plants was developed. a population of putative transformed chrysanthemum plants ... | 1995 | 24186624 |
| phylogenetic analysis of new zealand tomato spotted wilt virus isolates suggests likely incursion history scenarios and mechanisms for population evolution. | tomato spotted wilt virus (tswv) is an internationally significant pathogen with a wide host range, vectored by thrips. we have studied the sequence variation and evolutionary mechanisms at play in parts of the l, m and s subgenomes of 23 new zealand tswv isolates collected between 1992 and 2009, aiming to identify the possible geographic origins of isolates. maximum-likelihood-based phylogenetic analyses of new zealand and overseas tswv isolates placed the l and m subgenome sequences of two iso ... | 2014 | 24232914 |
| mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus tospovirus, family bunyaviridae). | tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. the nss gene encoded by tospoviruses acts as a suppressor of host plant defense. we identified amino acid motifs that are conserved in all of the nss proteins of tospoviruses for which the sequence is known. using tomato spotted wilt virus (tswv) as a model, the role of these motifs in suppressor activity of nss was investigated. using site-directed point mutations in two conserved moti ... | 2014 | 24363189 |
| disruption of vector transmission by a plant-expressed viral glycoprotein. | vector-borne viruses are a threat to human, animal, and plant health worldwide, requiring the development of novel strategies for their control. tomato spotted wilt virus (tswv) is one of the 10 most economically significant plant viruses and, together with other tospoviruses, is a threat to global food security. tswv is transmitted by thrips, including the western flower thrips, frankliniella occidentalis. previously, we demonstrated that the tswv glycoprotein gn binds to thrips vector midguts. ... | 2014 | 24405031 |
| plant 4/1 protein: potential player in intracellular, cell-to-cell and long-distance signaling. | originally isolated as a result of its ability to interact with the movement protein of tomato spotted wilt virus in a yeast two-hybrid system, the 4/1 protein is proving to be an excellent tool for studying intracellular protein trafficking and intercellular communication. expression of 4/1 in vivo is tightly regulated, first appearing in the veins of the cotyledon and later in the vasculature of the leaf and stem in association with the xylem parenchyma and phloem parenchyma. structural studie ... | 2014 | 24611067 |
| winter weeds as inoculum sources of tomato spotted wilt virus and as reservoirs for its vector, frankliniella fusca (thysanoptera: thripidae) in farmscapes of georgia. | thrips-transmitted tomato spotted wilt virus (tswv) has a broad host range including crops and weeds. in georgia, tswv is known to consistently affect peanut, tomato, pepper, and tobacco production. these crops are grown from march through november. in the crop-free period, weeds are presumed to serve as a green bridge for thrips and tswv. previous studies have identified several winter weeds as tswv and thrips hosts. however, their ability to influence tswv transmission in crops is still not co ... | 2014 | 24612539 |
| the nss protein of tomato spotted wilt virus is required for persistent infection and transmission by frankliniella occidentalis. | tomato spotted wilt virus (tswv) is the type member of tospoviruses (genus tospovirus), plant-infecting viruses that cause severe damage to ornamental and vegetable crops. tospoviruses are transmitted by thrips in the circulative propagative mode. we generated a collection of nss-defective tswv isolates and showed that tswv coding for truncated nss protein could not be transmitted by frankliniella occidentalis. quantitative reverse transcription (rt)-pcr and immunostaining of individual insects ... | 2014 | 24623427 |
| the movement protein (nsm) of tomato spotted wilt virus is the avirulence determinant in the tomato sw-5 gene-based resistance. | the avirulence determinant triggering the resistance conferred by the tomato gene sw-5 against tomato spotted wilt virus (tswv) is still unresolved. sequence comparison showed two substitutions (c118y and t120n) in the movement protein nsm present only in tswv resistance-breaking (rb) isolates. in this work, transient expression of nsm of three tswv isolates [rb1 (t120n), rb2 (c118y) and non-resistance-breaking (nrb)] in nicotiana benthamiana expressing sw-5 showed a hypersensitive response (hr) ... | 2014 | 24690181 |
| the tomato spotted wilt virus cell-to-cell movement protein (nsm ) triggers a hypersensitive response in sw-5-containing resistant tomato lines and in nicotiana benthamiana transformed with the functional sw-5b resistance gene copy. | although the sw-5 gene cluster has been cloned, and sw-5b has been identified as the functional gene copy that confers resistance to tomato spotted wilt virus (tswv), its avirulence (avr) determinant has not been identified to date. nicotiana tabacum 'sr1' plants transformed with a copy of the sw-5b gene are immune without producing a clear visual response on challenge with tswv, whereas it is shown here that n. benthamiana transformed with sw-5b gives a rapid and conspicuous hypersensitive resp ... | 2014 | 24720811 |
| broad-spectrum transgenic resistance against distinct tospovirus species at the genus level. | thrips-borne tospoviruses cause severe damage to crops worldwide. in this investigation, tobacco lines transgenic for individual wlm constructs containing the conserved motifs of the l rna-encoded rna-dependent rna polymerase (l) gene of watermelon silver mottle virus (wsmov) were generated by agrobacterium-mediated transformation. the wlm constructs included: (i) translatable wlm in a sense orientation; (ii) untranslatable wlmt with two stop codons; (iii) untranslatable wlmts with stop codons a ... | 2014 | 24811071 |
| coat protein mutations in an attenuated cucumber mosaic virus encoding mutant 2b protein that lacks rna silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. | in tobacco plants, the cucumber mosaic virus (cmv) pepo strain induces mosaic symptoms, including pale green chlorosis and malformed tissues. here, we characterized the involvement of 2b protein and coat protein (cp) in the development of mosaic symptoms. a 2b mutant (r46c) that lacks viral suppressor of rna silencing (vsr) activity showed an asymptomatic phenotype with low levels of virus accumulation. tomato spotted wilt virus nss protein did not complement the virulence of the r46c, although ... | 2014 | 24889248 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| effect of watermelon silver mottle virus on the life history and feeding preference of thrips palmi. | thrips-borne tospoviruses cause numerous plant diseases that produce severe economic losses worldwide. in the disease system, thrips not only damage plants through feeding but also transmit causative agents of epidemics. in addition, thrips are infected with tospoviruses in the course of virus transmission. most studies on the effect of tospoviruses on vector thrips have focused on the tomato spotted wilt virus-frankliniella occidentalis system. thus, we focused on another thrips-borne tospoviru ... | 2014 | 25010157 |
| possible involvement of eef1a in tomato spotted wilt virus rna synthesis. | tomato spotted wilt virus (tswv) is a negative-strand rna virus in the family bunyaviridae and propagates in both insects and plants. although tswv can infect a wide range of plant species, host factors involved in viral rna synthesis of tswv in plants have not been characterized. in this report, we demonstrate that the cell-free extract derived from one of the host plants can activate mrna transcriptional activity of tswv. based on activity-guided fractionation of the cell-free extract, we iden ... | 2014 | 25151062 |
| resistance breaking tomato spotted wilt virus isolates on resistant pepper varieties in italy. | in spring 2012, resistance breaking (rb) isolates of tomato spotted wilt virus (tswv) that overcome the resistance conferred by the tsw gene in different pepper hybrids have been recovered in different locations in southern italy (campania and apulia regions) in protected cultivation, about one month after transplant. the percentage of symptomatic plants was 5-10% and, only in particular cases of advanced stage of cultivation, it reached 30-50% at the end of cycle. all tswv isolates induced simi ... | 2013 | 25151838 |
| analysis of the a-u rich hairpin from the intergenic region of tospovirus s rna as target and inducer of rna silencing. | earlier work indicated that tomato spotted wilt virus (tswv) messenger transcripts, and not the (anti)genomic rnas, are targeted by the rna silencing machinery. here, the predicted au-rich hairpin (hp) structure encoded by the intergenic region (igr) of the tswv s rna, and present at the 3' end of viral mrnas, was analyzed as a target and inducer for rna silencing. virus-derived sirnas (vsirnas) purified from virus infected plants were found to derive from all three genomic rna segments but pred ... | 2014 | 25268120 |
| emergence of groundnut ringspot virus and tomato chlorotic spot virus in vegetables in florida and the southeastern united states. | groundnut ringspot virus (grsv) and tomato chlorotic spot virus (tcsv) are two emerging tospoviruses in florida. in a survey of the southeastern united states, grsv and tcsv were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south florida, and occurred sympatrically with tomato spotted wilt virus (tswv) in tomato and pepper in south florida. tswv was the only tospovirus detected in other survey locations, with the exceptions of grsv from tomato (solanum lyco ... | 2015 | 25317844 |
| the first complete genome sequences of two distinct european tomato spotted wilt virus isolates. | tomato spotted wilt virus (tswv) represents a major constraint to the production of important vegetable and ornamental crops in several countries around the world, including those in europe. in spite of their economic importance, european tswv isolates have only been partially characterized, and a complete genome sequence has not been determined yet. in this study, we completed the whole genome sequence of two distinct tswv isolates from italy, p105 and p202/3wt. the sequences of the l and m seg ... | 2015 | 25326756 |
| phylogenetic analysis of tomato spotted wilt virus (tswv) nss protein demonstrates the isolated emergence of resistance-breaking strains in pepper. | resurgence of tomato spotted wilt virus (tswv) worldwide as well as in hungary causing heavy economic losses directed the attention to the factors contributing to the outbreak of this serious epidemics. the introgression of tsw resistance gene into various pepper cultivars seemed to solve tswv control, but widely used resistant pepper cultivars bearing the same, unique resistance locus evoked the rapid emergence of resistance-breaking (rb) tswv strains. in hungary, the sporadic appearance of rb ... | 2015 | 25331341 |
| small rna profiles from virus-infected fresh market vegetables. | functional small rnas, such as short interfering rnas (sirnas) and micrornas (mirnas), exist in freshly consumed fruits and vegetables. these sirnas can be derived either from endogenous sequences or from viruses that infect them. symptomatic tomatoes, watermelons, zucchini, and onions were purchased from grocery stores and investigated by small rna sequencing. by aligning the obtained small rna sequences to sequences of known viruses, four different viruses were identified as infecting these fr ... | 2014 | 25389086 |
| genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus tospovirus. | chrysanthemum stem necrosis virus (csnv) is a member of a tentative tospovirus species. in this study, the complete genomic sequence of the japanese csnv isolate tcch07a was determined. the l rna is 8960 nt long and encodes the 331.0-kda rna-dependent rna polymerase. the m rna is 4828 nt long and encodes the 34.1-kda movement protein (nsm) and the 127.7-kda glycoprotein precursor (gn/gc). the s rna is 2949 nt long and encodes the 52.4-kda silencing suppressor protein (nss) and the 29.3-kda nucle ... | 2015 | 25427981 |
| evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates. | a tsw resistance-breaking tomato spotted wilt virus field isolate (tswv-p331) found in northern italy originated via reassortment from two evolutionary distinct tswv strains, as revealed by recombination and phylogenetic analysis. compared to the closest isolate present in the database, p331 nss protein carries an unusually high number of amino acid substitutions, but no differences in the nucleocapsid protein. despite these substitutions, p331 nss is a potent silencing suppressor. as shown by p ... | 2015 | 25433286 |
| supervised learning and prediction of spatial epidemics. | parameter estimation for mechanistic models of infectious disease can be computationally intensive. nsoesie et al. (2011) introduced an approach for inference on infectious disease data based on the idea of supervised learning. their method involves simulating epidemics from various infectious disease models, and using classifiers built from the epidemic curve data to predict which model were most likely to have generated observed epidemic curves. they showed that the classification approach cou ... | 2014 | 25457597 |