Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [effect of ribavirin on bunyavirus reproduction in cell culture and in an experiment on white mice]. | 1983 | 6419466 | |
| mixed infections with tick-borne viruses in a seabird colony in eire. | viruses were isolated from 2 tick species collected from the nesting areas of seabirds on great saltee island, eire. bunyaviruses of the uukuniemi serogroup were isolated from hard ticks (ixodes uriae and i. rothschildi), bunyaviruses of the hughes serogroup from soft ticks (ornithodoros maritimus), and orbiviruses of the kemerovo serogroup from i. uriae and o. maritimus. the results indicate that the bunyaviruses, but not the orbiviruses, show "tick specificity". neutralising activity against m ... | 1984 | 6421266 |
| localized conserved regions of the s rna gene products of bunyaviruses are revealed by sequence analyses of the simbu serogroup aino virus. | the complete nucleotide sequence has been determined for the s rna of aino virus, a member of the simbu serogroup (bunyavirus genus, family bunyaviridae). the s rna is 850 nucleotides long (2.76 x 10(5) daltons) and in the viral complementary sequence has a short 5' non-coding region of 34 nucleotides and a more extensive 3' non-coding region of 117 nucleotides. the 3'-5' complementarity of the aino s rna is about 25 residues long, depending on the arrangement. the aino sequence predicts that, l ... | 1984 | 6532000 |
| genome subunit reassortment among bunyaviruses analysed by dot hybridization using molecularly cloned complementary dna probes. | a simple and rapid procedure for determining the genotypes of viruses has been applied to analysis of genome subunit reassortment in heterologous crosses of batai virus, bunyamwera virus, and maguari virus, three members of the bunyamwera serogroup of bunyaviruses. the procedure for determining genotype made use of specific molecular probes to identify the parental origin of the l and m rna subunits. complementary dna copies of the three rna segments of bunyamwera virus were prepared by reverse ... | 1984 | 6547258 |
| [conditions for preserving the simbu group of bunyaviruses]. | 1983 | 6670261 | |
| la crosse bunyavirus can mediate ph-dependent fusion from without. | lipid binding properties which are dependent on exposure to acid ph are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. cell-to-cell fusion can be used to demonstrate this function. la crosse virus, a member of the family bunyaviridae, fused bhk-21 cells from without (ffwo) upon exposure of the absorbed virus to ph 6.3 or below. a high multiplicity of infection and temperature of 37 degrees were necessary for optimum fusion. the ph r ... | 1984 | 6695500 |
| a transcript from the s segment of the germiston bunyavirus is uncapped and codes for the nucleoprotein and a nonstructural protein. | analysis of the rnas present in bhk-21 cells infected with germiston virus showed that the transcripts from the l and m segments have a size similar to that of their template, whereas two types of complementary rna are transcribed from the s segment. one, s1, is a full-length "plus" rna strand (antigenome), and the other, s2, is an incomplete plus rna strand which serves as mrna for at least the synthesis of the n protein and a virus-specific nonstructural polypeptide, p12. the 5' ends of these ... | 1984 | 6699936 |
| mechanisms of bunyavirus virulence. comparative pathogenesis of a virulent strain of la crosse and an avirulent strain of tahyna virus. | to analyze mechanisms of virulence in the california serogroup bunyaviruses, the virulent la crosse/original (lac/original) strain was compared with the avirulent tahyna/181-57 strain. in suckling mice, both viruses were lethal upon intracerebral injection but differed markedly in their neuroinvasiveness following subcutaneous injection; 20 and 20,000 plaque-forming units, respectively, were equivalent to 1 subcutaneous ld50. the sequential course of infection was followed after subcutaneous inj ... | 1984 | 6708454 |
| characterization of two recombination-complementation groups of uukuniemi virus temperature-sensitive mutants. | with the aim of isolating temperature-sensitive (ts) mutants defective in virus maturation or glycoprotein transport, uukuniemi virus, a bunyavirus, was mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. out of 13 initial clones unable to grow at 39 degrees c (non-permissive temperature), five mutants which grew to titres above 10(7) p.f.u./ml at 32 degrees c (permissive temperature) were selected for further studies. the mutants fell into two coinciding recombination-complementation groups. ... | 1984 | 6726186 |
| antibody against viruses in maternal and cord sera: non-specific inhibitors are found to higher titre on the maternal side of the circulation. | pregnancies were identified in which maternal igg antibodies against rubella virus were not detectable by single radial haemolysis. twenty paired maternal/cord sera were then tested for haemagglutination-inhibiting (hi) activity against rubella virus without kaolin pretreatment of the sera. in the absence of specific antibody, the hi activity observed could thus be ascribed to the effect of non-specific inhibitors. the hi activity in maternal sera was significantly (p less than 0.001) higher tha ... | 1984 | 6736640 |
| carbon dioxide sensitivity of mosquitoes infected with california encephalitis virus. | four species of mosquitoes became sensitive to carbon dioxide approximately 3 to 4 days after they received intrathoracic injectins of california encephalitis virus. aedes melanimon and aedes dorsalis infected orally with california encephalitis virus also became carbon dioxide-sensitive, but mosquitoes infected transovarially did not. sensitivity to carbon dioxide was inhibited by antiserum to california encephalitis virus. to our knowledge this is the first report of carbon dioxide sensitivity ... | 1980 | 6773145 |
| tataguine virus infection in mice and hamsters. | suckling hamsters, weanling and suckling mice, inoculated intracerebrally with tataguine virus consistently developed fatal infections with average survival time of 3-8 days. deaths were observed in a few suckling hamsters infected by the intraperitoneal route. infectivity assays and fluorescent antibody staining of organs of infected hamsters showed that the brain was the only organ in which viral replication occurred. histopathological lesions found in the brain of such mice and hamsters infec ... | 1980 | 6776388 |
| characterization of the viral ribonucleic acids and structural polypeptides of anopheles a, bunyamwera, group c, california, capim, guama, patois, and simbu bunyaviruses. | analyses of the viral ribonucleic acids and structural polypeptides of 17-22 of the 119 accepted or proposed members of the bunyavirus genus of arboviruses (family bunyaviridae), have shown that from the standpoint of their structural components these viruses are highly comparable to each other. the average molecular weights for the three viral rna species (l, large, m, medium, s, small) of 17 bunyaviruses were 2.93 x 10(6) (l, range 2.7-3.1 x 10(6)), 2.0 x 10(6) (m, range 1.8-2.3 x 10(6)), and ... | 1980 | 6778231 |
| molecular basis of bunyavirus transmission by mosquitoes: role of the middle-sized rna segment. | in an examination of the molecular basis of oral transmission of bunyaviruses by mosquitoes., la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortant viruses were compared in their ability to be transmitted to laboratory mice by the natural mosquito vector of lac virus, aedes triseriatus. both lac virus and the reassortment viruses containing the middle-sized (m) segment from the lac parent were efficiently transmitted. in contrast, ssh virus and reassortment viruses containing the m rna f ... | 1981 | 6781068 |
| [bunyaviruses]. | 1981 | 6798775 | |
| comparison of the sequences and coding of la crosse and snowshoe hare bunyavirus s rna species. | the sequence of the s rna of la crosse bunyavirus was deduced from analyses of dna copies cloned in the escherichia coli plasmid pbr322. the s rna is 984 nucleotides in length, has a base ratio of 31.8% u, 27.0% a, 23.2% c, and 18.0% g, and codes for two distinct gene products that are read from overlapping reading frames in the viral complementary strand. the larger gene product (n, 26.5 x 10(3) daltons) contains 235 amino acids, and the smaller gene product (nss, 10.4 x 10(3) daltons) has 92 a ... | 1983 | 6834480 |
| bunyavirus gene structure - function relationships and potential for rna segment reassortment in the vector: la crosse and snowshoe hare reassortant viruses in mosquitoes. | modern technology now permits the elucidation of viral gene contributions to virus-vector interactions. such knowledge will undoubtedly permit determination of those mechanisms which function to preserve vector-borne virus disease cycle specificity and integrity in nature. in addition, the application of genetic techniques to reassortant virus generation in vectors should permit prediction of the evolutionary and epidemic potential of viruses in nature. | 1983 | 6867030 |
| multiple leader rnas and messenger rnas are transcribed from the la crosse virus small genome segment. | nucleotide sequencing has demonstrated that the small genome segment of bunyaviruses contains the genetic information for two viral proteins (n and nss) in overlapping reading frames (akashi and bishop, 1983; cabradilla et al., in press). using 3' end-labeled genome probes, la crosse virus (lac) infected cells were shown to contain three leader rnas, which start at position 1 and terminate at approximate positions 74, 95, and 115 from the 3' end of the genome. primer extension and s1 mapping stu ... | 1983 | 6871993 |
| relationship between glycoproteins of the viral envelope of bunyaviruses and antibody-dependent plaque enhancement. | hamster antisera against three parental bunyaviruses, batai, bunyamwera and maguari viruses, and six recombinant viruses which carried the nucleocapsid protein of one parent and the glycoproteins of the other, have been tested for their interaction with each of the nine viruses under study by two assays, plaque reduction neutralization and antibody-dependent plaque enhancement. neutralization was clearly related to the specificity of the parental glycoproteins rather than the nucleoprotein, but ... | 1982 | 6890986 |
| molecular basis of bunyavirus per os infection of mosquitoes: role of the middle-sized rna segment. | the molecular basis of bunyavirus per os infection of mosquitoes was determined; la crosse (lac), snowshoe hare (ssh), and lac-ssh reassortment viruses were compared for their ability to infect aedes triseriatus, the natural vector of the lac virus. the viruses were comparable in their ability to infect midgut cells; 115 of 117 (98%) mosquitoes ingesting viruses containing the lac middle-sized rna segment and 92/100 (92%) of mosquitoes ingesting viruses containing the ssh middle-sized rna segmen ... | 1982 | 6951175 |
| recombination and gene coding assignments of bunyaviruses and arenaviruses. | 1980 | 7013623 | |
| the complete sequence and coding content of snowshoe hare bunyavirus small (s) viral rna species. | the complete sequence of the small (s) viral rna species of snowshoe hare (ssh) bunyavirus has been determined, principally from a dna copy of the rna cloned in the e.coli plasmid pbr322. the viral s rna (negative sense strand) is 982 nucleotides long (3.3 x 10(5) daltons) with complementary 5' and 3' end sequences. it has a base composition of 30.5%u, 25.8%a, 24.9%c and 18.7%g. in the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine c ... | 1982 | 7050911 |
| identification of virus-coded nonstructural polypeptides in bunyavirus-infected cells. | analyses of bunyavirus-infected cell extracts identified at least two virus-induced nonstructural polypeptides. with snowshoe hare (ssh), la crosse (lac), and six ssh-lac reassortant viruses, it was shown that one of these nonstructural polypeptides (nss, approximate molecular weight, 7.4 x 10(3)) is coded by the ssh small (s)-size viral rna species. this nonstructural polypeptide was not detected (at least in the same relative abundancies) in lac virus-infected cells or in cells infected with r ... | 1982 | 7077749 |
| nucleotide sequence analyses and predicted coding of bunyavirus genome rna species. | we performed 3' rna sequence analyses of [(32)p]pcp-end-labeled la crosse (lac) virus, alternate lac virus isolate l74, and snowshoe hare bunyavirus large (l), medium (m), and small (s) negative-stranded viral rna species to determine the coding capabilities of these species. these analyses were confirmed by dideoxy primer extension studies in which we used a synthetic oligodeoxynucleotide primer complementary to the conserved 3'-terminal decanucleotide of the three viral rna species (clerx-van ... | 1982 | 7086954 |
| electron microscopic study of bluegill virus. | bluegill virus (bgv) grown in bf-2 cells was studied by negative staining and ultrathin sections of infected cells. although bgv resembles bunyaviruses in gross physical appearance, it differs from this group in several important aspects. thus, bgv cannot be classified as a member of the bunyaviridae family and could be a representative of a novel family of enveloped rna viruses. | 1982 | 7093819 |
| mutant identifying a third recombination group in a bunyavirus. | only two recombination groups have been reported in genetic analyses of ts mutants of 10 different bunyaviruses from the bunyamwera and california encephalitis serogroups, although three groups are expected from the tripartite structure of the genome of all members of the family bunyaviridae. we describe now a ts mutant of maguari virus, magts23(iii), which recombined in both vertebrate (bhk-21) and invertebrate (aedes albopictus) cells with mutants representing recombination groups i and ii of ... | 1982 | 7097862 |
| characterization of monoclonal antibodies against the g1 and n proteins of lacrosse and tahyna, two california serogroup bunyaviruses. | 1982 | 7101727 | |
| the 3' terminal rna sequences of bunyaviruses and nairoviruses (bunyaviridae): evidence of end sequence generic differences within the virus family. | the 3' terminal nucleotide sequences of the three virus rna species of viruses representing eight serogroups of bunyaviruses (genus bunyavirus, bunyaviridae) and six serogroups of nairoviruses (genus nairovirus, bunyaviridae) have been characterized. members of the bunyavirus genus have conserved 3' end sequences (generally, 3' ucaucacauga...) that differ from the conserved 3' end sequences of members of the nairovirus genus (generally, 3' agaguuucu...). | 1982 | 7119754 |
| panhandles and hairpin structures at the termini of germiston virus rnas (bunyavirus). | 1982 | 7135833 | |
| characterization of the oligosaccharides of inkoo virus envelope glycoproteins. | inkoo virus (a bunyavirus) was grown in bhk-21 cells and labelled with [35s]methionine or [3h]mannose. [35s]methionine labelled the two envelope glycoproteins g1 (mr = 125000) and g2 (mr = 35000), as well as the nucleocapsid protein n (mr = 25000). only g1 and g2 were labelled with the sugar precursor. the [3h]mannose-labelled virus was solubilized with detergent and digested with pronase. the structure of the labelled glycopeptides originating from the mixture of g1 and g2 was studied by degrad ... | 1982 | 7153764 |
| analyses of patois group bunyaviruses: evidence for naturally occurring recombinant bunyaviruses and existence of immune precipitable and nonprecipitable nonvirion proteins induced in burnyavirus-infected cells. | 1981 | 7222477 | |
| attenuation of virulence of a bunyavirus involving an l rna defect and isolation of lac/ssh/lac and lac/ssh/ssh reassortants. | 1981 | 7233829 | |
| formation of reassortant bunyaviruses in dually infected mosquitoes. | 1981 | 7245612 | |
| radioimmune assays and molecular studies that place anopheles b and turlock serogroup viruses in the bunyavirus genus (bunyaviridae). | molecular analyses indicate that turlock virus (tur, turlock serogroup) and boraceia virus (bor, anopheles b serogroup) have virion rna species and polypeptides comparable in size to those of members of the bunyavirus genus and unlike those of members of the newly defined phlebovirus, nairovirus, or uukuvirus genera (bunyaviridae). the 11 terminal 3' end nucleotides of the three virion rna species of both bor and tur viruses (houcaucacaug...) are identical in sequence to the 3' end sequences of ... | 1981 | 7258499 |
| structural polypeptides of hazara virus. | four structural polypeptides of hazara virus, an agent closely related to the crimean-congo haemorrhagic fever (c-chf) viruses, were resolved by sds-polyacrylamide gel electrophoresis. three glycoproteins were identified (mol. wt. 84,000, 45,000 and 30,000) and were found to be associated with the virion envelope. a fourth polypeptide (mol. wt. 52,000) was non-glycosylated and associated with the nucleocapsid. the structural proteins of hazara virus differ markedly from those reported for other ... | 1981 | 7276913 |
| role of the middle-sized bunyavirus rna segment in mouse virulence. | 1981 | 7281516 | |
| comparisons of belmont virus, a possible bunyavirus unique to australia, with bunyamwera virus. | belmont virus is an arbovirus isolated from mosquitoes and has a preference for marsupial hosts. the diameter of virions by negative staining (122 nm before fixation and 91 nm after fixation) was greater than that of bunyamwera virus (94 nm and 79 nm respectively). however, the particles of both viruses appeared morphologically identical and sedimented at the same rate in sucrose density gradients. belmont virus had a tripartite segmented rna genome (28s, 24s and 11s) similar to bunyamwera virus ... | 1981 | 7288402 |
| proteins and glycoproteins specified by bunyamwera virus and by belmont virus, a possible bunyavirus, in mammalian cells. | purified preparations of belmont virus were shown to be very similar morphologically and biochemically to those of bunyamwera virus. comparisons of the synthesis of virus-specified proteins in vero and bhk-21 cells confirm the close taxonomic relationship. total protein synthesis was inhibited 95% by 23 h post-infection with belmont virus; a similar reduction occurred earlier in bunyamwera virus-infected cells. this inhibition was multiplicity dependent, and synthesis of the host protein compone ... | 1981 | 7288403 |
| structural characteristics of nairoviruses (genus nairovirus, bunyaviridae). | viruses from six antigenic groups of arthropod-borne viruses [crimean--congo haemorrhagic fever (cchf), nairobi sheep disease (nsd), qalyub (qyb), sakhalin (sak), dera ghazi khan (dgk) and hughes (hug) serogroups], some previously categorized as bunyavirus-like viruses and others previously ungrouped, have recently been assembled by serological analyses into a new genus of viruses (nairovirus genus) in the bunyaviridae. molecular studies of the virion rna and viral polypeptides have been underta ... | 1981 | 7299367 |
| orbiviruses and bunyaviruses from a seabird colony in scotland. | viruses isolated from ticks (ixodes uriae) and a kittiwake (rissa tridactyla) from a seabird colony at st. abb's head, scotland, were shown by complement fixation tests (cft) to be antigenically related to the uukuniemi and kemerovo serogroups. electron microscopic examination of cell cultures infected with the kemerovo group viruses revealed particles characteristic of orbiviruses, 72 +/- 3 nm in diam., with an inner core 37 +/- 3 nm in diam., in association with intracytoplasmic, densely stain ... | 1981 | 7320703 |
| antigenic relationships among simbu serogroup (bunyaviridae) viruses. | antigenic relationships among 24 bunyaviruses of the simbu serogroup were determined by complement-fixation (cf), serum dilution-plaque reduction neutralization (n) and, where possible, hemagglutination-inhibition (hi) tests. by cf, three distinct complexes of closely related viruses were identified within the serogroup. nola and thimiri viruses, which showed little relationship with other members of the serogroup, may represent two additional complexes. n tests in vero cells showed that individ ... | 1981 | 7325288 |
| evidence from recombinant bunyavirus studies that the m rna gene products elicit neutralizing antibodies. | 1980 | 7368566 | |
| the effect of glycosylation inhibitors on the maturation and intracellular polypeptide synthesis induced by snowshoe hare bunyavirus. | 1980 | 7368577 | |
| analyses of the 3'-terminal sequences of snowshoe hare and la crosse bunyaviruses. | 1980 | 7423860 | |
| monoclonal anti-fc receptor igg blocks antibody enhancement of viral replication in macrophages. | flaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes (ref. 1, and j.s.m.p. and j.s.p., unpublished observations) and in p388 d1 and other macrophage cell lines. a comparable phenomenon has been demonstrated with alphaviruses and bunyaviruses in p388 d1 cells, (j.s.m.p. and j.s.p., unpublished observations) but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ade ... | 1981 | 7453820 |
| [mosquito-borne arboviruses in the baikal region]. | a total of 20 virus strains were isolated from arthropods and rodents in the baikal region in 1992. thirteen of these were california serogroup viruses, 4 batai virus strains (bunyaviridae, bunyavirus), and 3 gheta virus strains (togaviridae, alphavirus, semliki complex). the rates of mosquito infection with california, batai, and gheta viruses were 1:6500, 1:24000, and 1:24000, respectively. the results of the neutralization test indicate the similarity of the isolated california complex strain ... | 1995 | 7483568 |
| rift valley fever virus l segment: correction of the sequence and possible functional role of newly identified regions conserved in rna-dependent polymerases. | the sequence of rift valley fever virus l segment that we published in a previous paper was erroneous in the 3'-terminal region of the antigenomic rna molecule. here, we have shown that the l segment is in fact 6404 nucleotides long and encodes a polypeptide of 237.7k in the viral complementary sense. sequence comparisons performed between the rna-dependent rna polymerases of 22 negative-stranded rna viruses revealed the existence of two novel regions located at the amino termini of the proteins ... | 1994 | 7515937 |
| transcription of a recombinant bunyavirus rna template by transiently expressed bunyavirus proteins. | we describe a convenient system for analyzing bunyavirus transcription using a recombinant rna template derived from the plasmid pbunscat, which comprises a negative-sense reporter gene (chloramphenicol acetyltransferase or cat) flanked by the exact 5' and 3' untranslated regions of the bunyamwera virus (bun) s rna segment. when cells which expressed bunyavirus proteins (either by recombinant vaccinia viruses or by the vaccinia virus-t7 system) were transfected with bunscat rna, cat activity cou ... | 1995 | 7544044 |
| [searching for hepatitis c virus by immunoelectron microscopy and its morphology]. | many investigators have searched for the causative agent(s) of blood-borne non-a, non-b hepatitis in sera and liver from patients and experimental animals, and have reported various virus-like particles, morphologically resembling parvovirus, togavirus, picornavirus, hepadnavirus, paramyxovirus, papovavirus, retrovirus or bunyavirus. despite extensive effort, none of these virus-like particles has been confirmed universally as an etiologic virus because of the absence of immunological identifica ... | 1995 | 7563652 |
| effect of heparin on hemagglutination by akabane and aino viruses belonging to the simbu group of bunyaviruses. | heparin inhibited the hemagglutinin activity of akabane and aino viruses. the minimal inhibitory concentration of heparin required to inhibit 8 hemagglutination (ha) u of akabane and aino viruses was 10 u/ml. goose erythrocytes failed to combine with the ha inhibitory factor of heparin. on the other hand, goose erythrocytes treated with heparinase had greatly reduced agglutinability by akabane virus. virus-heparin complex formation was observed by sedimenting heparin with the virus particles. | 1995 | 7571376 |
| isolation of potosi virus from aedes albopictus in north carolina. | a total of 4,169 adult mosquitoes were aspirated from 3 tire disposal sites in north carolina for virus assays. aedes albopictus was the dominant species, with a relative abundance of approximately 99%. potosi virus was isolated from one pool of 68 female ae. albopictus. priorities for future potosi virus research and the implications of the north carolina isolate are discussed. | 1995 | 7595449 |
| homodimeric association of the spike glycoproteins g1 and g2 of uukuniemi virus. | we have studied the interactions of the g1 and g2 membrane glycoproteins of uukuniemi virus, a bunyavirus, in virus particles and in triton x-100-solubilized virus. the g1 glycoprotein in intact virus or in triton solution could be oxidized into a covalent homodimer using cu2+ ion as a catalyst. immunoprecipitations of the glycoproteins from triton-solubilized virus lysates showed that g1 and g2 do not form a stable heterodimeric or heterooligomeric complex. the oligomeric association of g1 and ... | 1995 | 7645217 |
| bluetongue and douglas virus activity in new south wales in 1989: further evidence for long-distance dispersal of the biting midge culicoides brevitarsis. | 1995 | 7661825 | |
| cache valley virus. | cache valley virus (cvv) is a causative agent of a mosquito-borne disease syndrome of sheep and, possibly, of all ruminants, characterized by embryonic and fetal death, stillbirths, and multiple congenital malformations. cvv is endemic in canada, mexico, and the united states. several related bunyaviruses also may play a role in syndromes of congenital malformations and embryonic losses in north america. | 1994 | 7728634 |
| akabane virus. | akabane virus, an arthropod-borne bunyavirus, is the major cause of epizootics of congenital malformations in ruminants in australia, japan, korea, and israel, and is suspected to be a cause of sporadic outbreaks elsewhere. blood-sucking insects, such as biting midges, transmit the virus horizontally to vertebrates. climatic factors influence the seasonal activity and geographic range of the vector population and, therefore, occurrence of related disease. inoculated ruminants seroconvert rapidly ... | 1994 | 7728635 |
| protection from la crosse virus encephalitis with recombinant glycoproteins: role of neutralizing anti-g1 antibodies. | la crosse virus, a member of the california serogroup of bunyaviruses, is an important cause of pediatric encephalitis in the midwestern united states. like all bunyaviruses, la crosse virus contains two glycoproteins, g1 and g2, the larger of which, g1, is the target of neutralizing antibodies. to develop an understanding of the role of each of the glycoproteins in the generation of a protective immune response, we immunized 1-week-old mice with three different preparations: a vaccinia virus re ... | 1995 | 7745694 |
| bluetongue and douglas virus activity in new south wales in 1989: further evidence for long-distance dispersal of the biting midge culicoides brevitarsis. | infection of cattle with bluetongue and douglas viruses was detected on the central and southern coast of new south wales from january to april 1989. bluetongue virus infection was found well south of areas of expected occurrence. evidence is presented to support wind-borne dispersal of infected vectors, culicoides brevitarsis, southwards from the hunter valley. | 1995 | 7779035 |
| [nephropathia epidemica. hantavirus nephritis--a differential diagnosis in acute abdomen]. | nephropathia epidemica (ne) is an infectious disease caused by hantavirus of the bunyavirus family and carried by little rodents, in denmark presumably by the bank vole (clethrionomys glareolus). the disease usually presents with self-limiting renal failure, thrombocytopenia, fever, lower back and/or abdominal pain. as such it might be confused with for example acute abdomen as shown by the two cases given. final diagnosis is based upon demonstrating antibody formation against hantavirus. | 1994 | 7810017 |
| completion of the la crosse virus genome sequence and genetic comparisons of the l proteins of the bunyaviridae. | la crosse virus is a member of the bunyavirus genus in the family bunyaviridae, viruses with trisegmented rna genomes of mostly negative polarity composed of large (l), medium (m), and small (s) segments. the sequences of the la crosse/original m and s rna segments have been previously characterized. using reverse transcriptase in conjunction with pcr amplification, we have obtained the nucleotide sequence of the l rna segment, which encodes the viral polymerase in a single large open reading fr ... | 1995 | 7831835 |
| an rna-dependent rna polymerase activity associated with virions of tomato spotted wilt virus, a plant- and insect-infecting bunyavirus. | an rna-dependent rna polymerase activity was found associated with virions of tomato spotted wilt virus (tswv), a plant- and insect-infecting member of the family bunyaviridae. radiolabeled nucleoside triphosphates were incorporated into trichloroacetic acid-precipitable products by detergent-disrupted, purified tswv virions. incorporation was reduced to near-background levels when rnase was present in the reaction mixture. the predominantly double-stranded rna products were rnase-resistant at h ... | 1995 | 7871744 |
| determination and comparative analysis of the small rna genomic sequences of california encephalitis, jamestown canyon, jerry slough, melao, keystone and trivittatus viruses (bunyaviridae, genus bunyavirus, california serogroup). | the nucleotide sequences of the small (s) genomic rnas of six california (cal) serogroup bunyaviruses (bunyaviridae: genus bunyavirus) were determined. the s rnas of two california encephalitis virus strains, two jamestown canyon virus strains, jerry slough virus, melao virus, keystone virus and trivittatus virus contained the overlapping nucleocapsid (n) and non-structural (nss) protein open reading frames (orfs) as described previously for the s rnas of other cal serogroup viruses. all n prote ... | 1995 | 7897347 |
| viruses isolated from mosquitoes collected in sri lanka. | attempts to isolate viruses from 178,181 unengorged female mosquitoes collected from different ecologic areas of sri lanka yielded 31 isolates: 17 of japanese encephalitis (je) virus, nine of getah virus, three of a batai-related bunyavirus, and two of arkonam virus. culex tritaeniorhynchus and mansonia uniformis mosquitoes were found to carry je virus in a dry zone nonepidemic area, and cx. pseudovishnui was found to carry it in a wet zone nonepidemic area. japanese encephalitis virus was isola ... | 1994 | 7915499 |
| localization of bunyamwera bunyavirus g1 glycoprotein to the golgi requires association with g2 but not with nsm. | the bunyamwera bunyavirus (bun) m rna genome segment encodes three proteins, two glycoproteins termed g1 and g2 and a non-structural protein called nsm, in the form of a polyprotein precursor that is co-translationally cleaved to give the mature proteins. indirect immunofluorescence experiments have shown that these proteins localize to the golgi complex in bun-infected cells. we have used a recombinant vaccinia virus (vtf7-3), which expresses bacteriophage t7 rna polymerase, to drive the expres ... | 1994 | 7996137 |
| [the antiviral activity of inhibitors of adp ribosylation in experimental alpha- and bunyavirus infections]. | the prophylactic and therapeutic effects of adp-ribosylation inhibitors, 3,n-acetylaminobenzamide and 3,n-butyrylaminobenzamide, were studied in mice inoculated with an alphavirus or a bunyavirus. both drugs were shown to have high levels of antiviral activity when given as a single subcutaneous injection in a dose of 10 mg/kg while increasing the number of injections did not increase their efficacy or might even decrease it. | 1994 | 8017062 |
| arboviruses causing human disease in the australasian zoogeographic region. | over 65 arboviruses have been reported from countries in the australasian zoogeographic region, but only a few have been implicated in human disease. these include the flaviviruses murray valley encephalitis (mve), kunjin (kun), kokobera (kok), and dengue, particularly types 1 and 2; the alphaviruses ross river (rr), barmah forest (bf), and sindbis (sin); and the bunyaviruses, gan gan and trubanaman. in this paper recent epidemiological and clinical results pertaining to these viruses are review ... | 1994 | 8031248 |
| a signal for golgi retention in the bunyavirus g1 glycoprotein. | the g1 and g2 glycoproteins of punta toro virus, a member of the bunyaviruses, are targeted to the golgi complex, where viral budding occurs. we found that the g1 protein, when expressed in the absence of g2, is also targeted to the golgi complex. a series of g1 proteins truncated at the carboxyl-terminal region was constructed, and the localization of the expressed proteins was examined. it was found that the proteins expressed from constructs with partial deletions in the cytoplasmic domain we ... | 1994 | 8077205 |
| vector competence of selected mosquito species (diptera: culicidae) for california strains of northway virus (bunyaviridae: bunyavirus). | selected mosquito species from central valley, coastal, and alpine habitats of california were evaluated for their vector competence for northway (nor) virus. culiseta incidens thomson, culiseta inornata (williston), and anopheles freeborni aitken were the only competent vectors when fed virus. aedes sierrensis (ludlow), as well as alpine snow pool aedes (i.e., ae. cataphylla dyar, ae. hexodontus dyar, ae. increpitus dyar and ae. tahoensis dyar), ae. melanimon dyar, ae. washinoi lanzaro & eldrid ... | 1993 | 8099625 |
| medically important arboviruses of the united states and canada. | of more than 500 arboviruses recognized worldwide, 5 were first isolated in canada and 58 were first isolated in the united states. six of these viruses are human pathogens: western equine encephalitis (wee) and eastern equine encephalitis (eee) viruses (family togaviridae, genus alphavirus), st. louis encephalitis (sle) and powassan (pow) viruses (flaviviridae, flavivirus), lacrosse (lac) virus (bunyaviridae, bunyavirus), and colorado tick fever (ctf) virus (reoviridae, coltivirus). their scien ... | 1994 | 8118792 |
| the s rna genome segments of batai, cache valley, guaroa, kairi, lumbo, main drain and northway bunyaviruses: sequence determination and analysis. | bunyaviruses have a genome comprising three segments of negative-sense rna. the smallest rna segment, s, encodes the nucleocapsid protein, n, and a nonstructural protein, nss, in overlapping reading frames. the sequences of the s genome rna segments of seven bunyaviruses (batai, cache valley, guaroa, kairi, main drain, northway and lumbo) were determined from cloned cdnas obtained using a one-step reverse transcription-pcr protocol. these sequences were compared to those of six viruses previousl ... | 1994 | 8126455 |
| replication in cultured c2c12 muscle cells correlates with the neuroinvasiveness of california serogroup bunyaviruses. | the neuroinvasiveness of california serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. neuroinvasiveness was mapped to the medium (m) rna segment of the virus, which encodes the viral glycoproteins, when reassortants were made between la crosse/original virus, a neuroinvasive isolate, and tahyna-181/57 virus, a nonneuroinvasive clone. we have tested the murine muscle cell line c2c12 as a surrogate for myotropism ... | 1994 | 8184551 |
| detection of the l protein of tomato spotted wilt virus. | the 5'-terminal and 3'-terminal parts of the single open reading frame (orf) in the l rna of tomato spotted wilt virus (tswv) were expressed using a prokaryotic expression system. using antibodies raised against the translational products obtained a 330-kda protein could be specifically detected in preparations of purified virions and in nucleocapsid preparations from tswv-infected leaf tissue. the results obtained indicate that the l protein of tswv, though much larger than that of the animal-i ... | 1993 | 8212587 |
| molecular determinants of the virulence and infectivity of california serogroup bunyaviruses. | california bunyaviruses cause encephalitis in mammalian hosts after peripheral infection. the virulence of these viruses is determined by their ability to replicate sequentially in striated muscle, cause viremia, and invade and replicate in the central nervous system. these viruses are also able to infect vector mosquitoes following ingestion of a blood meal containing virus. bunyaviruses are negative stranded rna viruses with a trisegmented genome, and the large, medium, and small rna segments ... | 1993 | 8257095 |
| inhibitory effect of heparin on rhabdovirus and bunyavirus isolated from cattle. | 1993 | 8368970 | |
| non-viral sequences at the 5' ends of dugbe nairovirus s mrnas. | analyses of the mrna transcription processes of viruses in four genera (bunyavirus, hantavirus, phlebovirus and tospovirus) of the family bunyaviridae have revealed a common mechanism of initiation using host-derived primers, known as cap-snatching. to provide similar information on the fifth genus in the family, the 5' ends of dugbe nairovirus s mrna species were specifically cloned and sequenced. this revealed the presence of non-viral heterogeneous sequences, five to 16 nucleotides in length ... | 1993 | 8409954 |
| characterization of bunyamwera virus s rna that is transcribed and replicated by the l protein expressed from recombinant vaccinia virus. | analysis of the 5' termini of bunyamwera virus s segment mrnas by cloning and sequence analysis revealed the presence of nonviral, heterogeneous sequences 12 to 17 bases long. this is similar to reports for other members of the family bunyaviridae and is taken to indicate that mrna transcription is primed by a "cap-snatching" mechanism. the 3' end of the bunyamwera virus s mrna was mapped, by using an rnase protection assay, to 100 to 110 nucleotides upstream of the 3' end of the template. previ ... | 1993 | 8437222 |
| viruses of chelonia. | viruses occurring in turtles and tortoises are hetergeneous but according to ecologic characteristics and pathogenic properties they can be divided in two major groups: 1. arboviruses (toga-, flavi-, rhabdo- and bunyaviruses) transmitted by arthropods cause severe diseases in homoiothermic vertebrates. the viruses are of great epidemiological interest in human and veterinary medicine. chelonia and other reptiles infected by bites of vectors e.g. aedes, anopheles, culex develop cyclic viremia wit ... | 1993 | 8456570 |
| toscana virus genomic l segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand rna viruses. | the complete nucleotide sequence of toscana (tos) virus (bunyaviridae, phlebovirus) l segment was determined. the l segment is 6404 nucleotides long, containing a single open reading frame (orf) in the viral complementary sense coding for a protein of 2095 amino acids that, as in the case of negative strand rna viruses, could be part of the rna polymerase of tos virus. this orf is expressed by a messenger rna (mrna) as long as the genomic segment. like the mrnas expressed by the genomic segments ... | 1993 | 8460526 |
| characterization of murine caraparu bunyavirus liver infection and immunomodulator-mediated antiviral protection. | a rapid, peripheral disease model utilizing the bunyavirus, caraparu, was established in mice for the evaluation of antiviral therapy with immunomodulators. 4-6-week-old b6c3f1 female mice, inoculated intraperitoneally with virus, developed coagulative liver necrosis and died between 4-6 days after infection. this caraparu disease model was relatively resistant to treatment with immunomodulators, such as abmp, ampligen, alpha-interferon (ifn-alpha) or beta-interferon (ifn-beta). however, a signi ... | 1993 | 8460932 |
| the nucleotide sequence of the m rna segment of tomato spotted wilt virus, a bunyavirus with two ambisense rna segments. | 1993 | 8468562 | |
| polygenic control of neuroinvasiveness in california serogroup bunyaviruses. | the pathogenesis of the california serogroup bunyaviruses includes both extraneural and intraneural replicative phases that can be separated experimentally. the present study dissects the viral genetic determinants of extraneural replication. we have previously described two attenuated reassortant clones of california serogroup bunyaviruses which exhibit reduced neuroinvasiveness after subcutaneous inoculation into suckling mice. clone b1-1a bears an attenuated middle rna segment (neuroinvasiven ... | 1993 | 8510208 |
| isolation of batai virus (bunyaviridae:bunyavirus) from the blood of suspected malaria patients in sudan. | from august through november 1988, 77,500 patients with fever presented to the municipal hospital and to eight government health centers in kassala, a town of approximately 400,000 individuals in eastern sudan. a diagnosis of malaria, based primarily on clinical presentation, was made in 14,395 individuals during this four-month period; fevers of unknown origin were diagnosed in 29 patients. a bunyavirus that was antigenically similar or identical to batai virus by complement fixation and plaque ... | 1993 | 8517485 |
| inhibition of bunyaviruses, phleboviruses, and hantaviruses by human mxa protein. | viruses of the bunyaviridae family cause a variety of diseases ranging from uncomplicated fever to potentially lethal encephalitis and hemorrhagic fever. little is known about the factors determining pathogenicity in the vertebrate host. interferons have been reported to be inhibitory, but their mode of action against members of the bunyaviridae has not yet been elucidated. the interferon-induced mxa protein encoded on human chromosome 21 is a large gtpase with antiviral activity against distinc ... | 1996 | 8551631 |
| tropism of bunyaviruses: evidence for a g1 glycoprotein-mediated entry pathway common to the california serogroup. | the california serogroup is composed of antigenically and biologically related viruses within the bunyavirus genus of the bunyaviridae. we used a large panel of murine cells to study their tissue tropisms and found virtually identical patterns of viral replication among all of the members of this serogroup, in contrast to other members of the family (bunyamwera, cache valley, and punta toro viruses). by analyzing the nonpermissive infections with both an rna dot-blot and a virus binding assay, w ... | 1995 | 8553534 |
| hantaviruses: clinical, microbiologic, and epidemiologic aspects. | hantaviruses comprise a genus of the family bunyaviridae. bunyaviruses are enveloped viruses with a negative-sense, tripartite rna genome. hantaviruses are etiologic agents for two acute and severe illnesses of man, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). each hantavirus is primarily associated with a single rodent host species or genus, and is transmitted to man through accidental inhalation or ingestion of virus-contaminated rodent excreta. the dis ... | 1995 | 8561891 |
| typing of lacrosse, snowshoe hare, and tahyna viruses by analyses of single-strand conformation polymorphisms of the small rna segments. | single-strand conformation polymorphism analysis was developed to differentiate the small rna segments of three california serogroup bunyaviruses. the small rna segments of la crosse, snowshoe hare, and tahyna viruses were reverse transcribed and pcr amplified. the cdnas were then denatured, rapidly chilled to promote intrastrand reassociation, separated electrophoretically on a nondenaturing gel at room temperature, and silver stained. the resulting single-strand conformation polymorphism patte ... | 1995 | 8586697 |
| the infectious bronchitis virus nucleocapsid protein binds rna sequences in the 3' terminus of the genome. | the infectious bronchitis virus (ibv) nucleocapsid protein was expressed as a bacterial fusion protein which differed from the native protein only in the addition of six amino terminus histidine residues. using rna overlay protein blot assays, the recombinant protein was shown to bind to rna fragments specific for the positive sense 3' noncoding end of the ibv genome. at greater concentrations of sodium chloride, the native and fusion nucleocapsid proteins similarly bound to g rna, representing ... | 1996 | 8599203 |
| molecularly engineered resistance to california serogroup virus replication in mosquito cells and mosquitoes. | introduction of genetic elements derived from a viral pathogen's genome may be used to reduce the vectorial capacity of mosquitoes for that virus. a double subgenomic sindbis virus expression system was utilized to transcribe sequences of lacrosse (lac) virus small (s) or medium (m) segment rna in sense or antisense orientation; wild-type sindbis and lacrosse viruses have single-stranded rna genomes, the former being positive sense and the latter being negative sense. recombinant viruses were ge ... | 1996 | 8633038 |
| analysis of the intracellular transport properties of recombinant la crosse virus glycoproteins. | the g1 and g2 glycoproteins of la crosse virus, a member of the bunyavirus genus of the bunyaviridae, are encoded as a single open reading frame (orf) in the viral middle-sized rna segment. the primary product from this orf is processed, either cotranslationally or shortly after translation, into the two glycoproteins and a nonstructural protein, nsm, of unknown function. we have expressed la crosse glycoproteins using vaccinia vectors and studied their processing and localization. when expresse ... | 1996 | 8661399 |
| geographic distribution and serologic and genomic characterization of morro bay virus, a newly recognized bunyavirus. | more than 75,000 immature mosquitoes in three genera were collected from coastal california, reared to the adult stage, and tested for virus by plaque assay in vero cell cultures. twenty-six strains of morro bay (mb) virus, a newly recognized member of the california (cal) serogroup, were isolated from aedes squamiger, a pestiferous salt marsh mosquito species restricted to intertidal salt marshes in coastal california and baja california. the geographic distribution of the isolates was 10 from ... | 1996 | 8686772 |
| detecting bunyaviruses of the bunyamwera and california serogroups by a pcr technique. | many bunyaviruses of the bunyamwera and california serogroups are medically important human pathogens. the development of an effective technique to detect the viruses by using molecular biologic tools, such as pcr, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. in this study, we evaluated eight pairs of primers for reactivity with 44 viruses of the genus bunyavirus, using a reverse transcriptase pcr technique. with a pair of serogroup-speci ... | 1996 | 8727900 |
| the s rna genomic sequences of inkoo, san angelo, serra do navio, south river and tahyna bunyaviruses. | the complete nucleotide sequences of the small (s) genomic rna segments of five california (cal) serogroup bunyaviruses (two inkoo virus strains, san angelo virus, serra do navio virus, south river virus and tahyna virus) were determined. in agreement with previously published data concerning cal serogroup viruses, the nucleocapsid (n) and non-structural (nss) proteins were encoded in over-lapping open reading frames (orfs). all n protein orfs were 708 nucleotides in length and encoded a 235 ami ... | 1996 | 8760423 |
| inkoo and tahyna, the european california serogroup bunyaviruses: sequence and phylogeny of the s rna segment. | inkoo (ink) and tahyna (tah) viruses, european representatives of the california serogroup (cal), genus bunyavirus, family bunyaviridae, are transmitted by mosquitoes and frequently infect man. the s segments of ink and tah prototype strains were amplified, cloned and sequenced. ink s consists of 986 and tah s of 977 nucleotides (nt) coding for a nucleocapsid protein of 235 amino acids (aa) and, in an overlapping reading frame, for a nonstructural protein of 92 or 97 aa, respectively. by s segme ... | 1996 | 8760424 |
| [ngari virus (bunyaviridae: bunyavirus). first isolation from humans in senegal, new mosquito vectors, its epidemiology]. | ngari virus (nri) (bunyaviridae, genus bunyavirus) was isolated first from male aedes simpsoni mosquitoes in southeastern senegal in 1979. then, it was recovered from several mosquito species in senegal, burkina faso, central african republic and madagascar. a potential pathogenicity of nri virus in humans was suspected when the virus was isolated from two patients in dakar in october and november 1993. the large diversity of culicidae vectors and feeding patterns showed a large heterogeneity of ... | 1996 | 8765950 |
| arbovirus surveillance in italy. | a high number of different arboviruses have been demonstrated to be present in italy, due to the coexistence of climates that are characteristic of continental areas in the north of the country and of subtropical areas in the south. viruses circulating in central europe, such as the tick-borne encephalitis (tbe) virus, are present in central and northern regions, whereas viruses circulating in mediterranean areas, such as the sandfly fever viruses are present in central and southern italy. virus ... | 1995 | 8778650 |
| use of the yellow fever virus vaccine strain 17d for the study of strategies for the treatment of yellow fever virus infections. | we have employed the attenuated vaccine strain 17d of yellow fever virus (yfv) to evaluate the inhibitory effect of a selected series of compounds on yfv in vero cells. use of the vaccine strain does not require high-level microbiological containment facilities and should allow extensive screening. in addition, yfv may serve as a model for other flaviviruses including hepatitis c virus (hcv), and thus strategies for the treatment of yfv infections may apply to flavivirus infections in general. i ... | 1996 | 8783804 |
| sandfly fever viruses in italy. | two serologically distinct agents, the sandfly fever sicilian and the sandfly fever naples viruses, were isolated by sabin from blood samples taken during an italian epidemic of febrile illness. since then, several different viruses have been isolated from sandflies and/or humans in both the old and new world. toscana virus, a new virus closely antigenically related to sandfly fever naples virus, was isolated in 1971 from the sandfly phlebotomus perniciosus in italy. extensive studies on the imp ... | 1996 | 8800804 |
| detection of california serogroup bunyaviruses in tissue culture and mosquito pools by pcr. | a reverse transcription-polymerase chain reaction (rt-pcr) protocol was developed for rapid detection of 14 california serogroup viruses using universal primer pairs. these primers are specific for the small rna (s rna) and middle rna (m rna) segments. the method has been employed to detect jamestown canyon (jc) virus in naturally infected mosquitoes. with this technique, it is possible to detect virus in an amount of material equivalent to as little as 0.03 mosquito. | 1996 | 8801229 |
| antiviral activities of tragacanthin polysaccharides on punta toro virus infections in mice. | tragacanthin polysaccharides from astragalus brachycentrus (av208) and astragalus echidnaeformis (av212) plants, which are devoid of in vitro antiviral activity, were evaluated in a mouse model of punta toro virus (ptv) infection. the ptv (a phlebovirus member of the bunyaviridae family of viruses) is a model for studying the treatment of rift valley fever and hantavirus infections. single intraperitoneal treatments with 12.5-200 mg/kg/day doses of av212 given 24 h before or 4 and 24 h after vir ... | 1996 | 8804797 |
| the complete nucleotide sequence and genome organization of the m rna segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. | the m rna of peanut bud necrosis virus (pbnv; synonym groundnut bud necrosis virus) is 4801 nucleotides in length. it comprised two orfs in an ambisense organization and terminal inverted repeats. the 3' large orf (3363 nucleotides in the virus-complementary strand) encoded a protein with a predicted size of 127.2 kda which was identified as the glycoprotein precursor (gp) of the g1 and g2 glycoproteins. a comparison of the deduced amino acid sequence of gp revealed 37% identity and 58-59% simil ... | 1996 | 8811036 |
| the role of deer as a possible reservoir host of potosi virus, a newly recognized arbovirus in the united states. | potosi (pot) virus (bunyaviridae) was isolated from aedes albopictus, an introduced asian mosquito species, collected at a used tire yard in potosi, missouri (usa), in august and september, 1989. in september, 1990, small animals were trapped at the tire yard and six cattle were sampled at an adjacent farm; in november 1990 and 1991, blood samples were collected with filter paper strips from 364 hunter-killed, white-tailed deer (odocoileus virginianus) in the region to determine the possible res ... | 1996 | 8827670 |