Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the effect of b-cell immunosuppression on age-related resistance of chickens to marek's disease. | chickens were bursectomized by cyclophosphamide treatment at hatching. at 8 or 9 weeks of age, bursectomized and unbursectomized hatchmates, free from prior infection, were challenged with pathogenic marek's disease virus. oncogenicity of the virus inoculum was confirmed by inoculating 1-day-old susceptible chickens. at the time of virus challenge, blood cells from the cyclophosphamide-treated chickens were able to mount a vigorous graft-versus-host reaction in allogeneic embryos. this ability i ... | 1975 | 234791 |
| marek's disease virus (kekava strain) replication in chickens, chick embryos and cell cultures. | in the course of 12 passages of marek's disease virus (mdv) strain kekava (mdv-kekava) in chickens, the morbidity varied greatly (from 23 to 50 percent). mdv-kekava produced plaques in cultures of chick embryo kidney and adult chicken kidney cells and chick embryo fibroblasts (cef). the virus adaptation to the cultures was very slow. mdv-kekava induced the formation of pocks on the chorioallantoic membranes (cam) of chick embryos but the proportion of embryos with cam lesions did not exceed 24 p ... | 1975 | 241226 |
| cell-mediated immunity to tumor antigen in marek's disease: susceptibility of effector cells to antithymocyte serum and enhancement of cytotoxic activity by vibrio cholerae neuraminidase. | spleen cells from chickens inoculated 7 to 8 days previously with marek's disease virus were cytotoxic for 51cr-labeled cells of a marek's disease lymphoblastoid cell line (msb-1 line) in a 4-h in vitro cytotoxic assay. the cytotoxic activity of spleen cells was inhibited by pretreatment with antithymocyte serum and complement, but not with complement alone or in combination with anti-bursa cell serum or normal preimmune serum. the conclusion was that the effector cell in the above cytotoxic ass ... | 1977 | 302827 |
| density-gradient separation of spleen-cell subpopulations from marek's disease virus-infected chickens. | ficoll gradient separation of spleen-cell suspensions has shown that depressed t-cell mitogen reactivity in cultures of jm-vs and md cells results from selective depletion of mitogen-responsive t-cells. | 1978 | 312761 |
| pathogenesis of marek's disease: early appearance of marek's disease tumor-associated surface antigen in infected chickens. | marek's disease tumor-associated surface antigen (matsa) appeared in different lymphoid tissues of p-line chickens soon after they were infected with bc-1 strain of marek's disease virus. matsa-bearing cells first appeared in spleens by 5 days post infection (pi) and were observed through a 21-day experimental period at mean levels varying from 3.8 to 21.9% of the total cells examined. lower percentages of matsa-bearing cells were observed in the thymus, in the bursa of fabricius, among peripher ... | 1978 | 357748 |
| atheroarteriosclerosis induced by infection with a herpesvirus. | atheroarteriosclerosis closely resembling that in humans was induced in normocholesterolemic and hypercholesterolemic chickens by infection with marek's disease herpesvirus (mdv). four comparably sized groups of chickens were used. each group was initially fed a diet relatively poor in cholesterol. group i and ii were inoculated intratracheally at 2 days of age with mdv. at 15 weeks, one group of virus-infected chickens (group ii) and one group of uninfected controls (group iv) were fed a 2% cho ... | 1979 | 382868 |
| immune responses of chickens fed the androgen analog mibolerone. | mibolerone, an androgen analog (17 beta-hydroxy-7-alpha, 17-dimethylestr-4-en-3-one), induces a slow but progressive involution of the bursa of fabricius when fed to chickens at microng levels during the first 7 weeks of life. chickens receiving mibolerone remained immunologically competent, evidenced by: 1) their antibody response to nonreplicating antigens and infectious antigens; 2) the number of antibody-producing cells in their spleens; 3) the stimulation of their peripheral leukocytes with ... | 1977 | 405963 |
| effects of vaccination for marek's disease on growth of a marek's-virus-induced transplantable lymphoma in syngeneic and allogeneic chickens. | a study was made of the effects of vaccination with turkey herpesvirus on the growth of tumors following inoculation of lymphoma cells induced by marek's disease virus (mdv). the cells used were from a transplantable mdv-induced lymphoid tumor (mdt-198) maintained by passaging in syngeneic hosts. vaccination did not prevent progressive tumor growth or alter subsequent mortality among syngeneic recipients of mdt-198 cells. with allogeneic recipients, however, vaccination had a significant protect ... | 1979 | 486007 |
| serum protein changes in chickens with experimental marek's disease. | serum protein alterations in chickens contact-exposed to marek's disease virus (mdv) were studied by crossed immunoelectrophoresis. total protein values of infected chickens were initially, i.e. in the 4th week, low and later not significantly changed from those of the controls. in all infected chickens a significant increase was seen in the igg components. three of 6 infected chickens showing a prolonged period of clinical illness presented the most pronounced changes in other serum proteins, m ... | 1979 | 523311 |
| pathogenicity of a marek's disease virus for chickens vaccinated with graded doses of herpesvirus of turkeys. | six different groups of one-day-old chickens were vaccinated with herpesvirus of turkeys (hvt) vaccine at doses of 0, 4, 16, 64, 256 and 1,024 plaqueforming units. at 9 days of age they were challenged with an acute strain of marek's disease herpesvirus (mdhv). gross and microscopic lesions in nerves and gonads were investigated and combined for quantitative expression of mdhv pathogenicity. including the microscopic lesions improved the accuracy of the hvt doses/mdhv response. the qualitative a ... | 1979 | 546406 |
| isolation and characterisation of herpes virus from turkeys. | turkey herpesvirus (hvt) was isolated from the kidneys of 6 of 8 turkey poults from two flocks. the isolates were identified by syncytial-type of cytopathology, inhibition of plaque formation by 5-bromodeoxyuridine, formation of cowdry type a intranuclear inclusions in cell cultures, and presence of herpes-type virions in negatively stained preparations and thin sections of infected cell cultures. one of these isolates inoculated into chickens proved apathogenic over an observation period of 10 ... | 1977 | 614022 |
| suppression of mitogen-induced proliferation of normal spleen cells by macrophages from chickens inoculated with marek's disease virus. | spleen cells from chickens 7 days after inoculation with marek's disease virus (mdv) responded poorly to stimulation by phytohemagglutinin (pha). addition of these cells to syngeneic normal spleen cells caused of marked suppression of the pha response of the normal cells. the mdv spleen cells also inhibited the dna synthesis of msb-1 lymphoblastoid cells in vitro. the suppressive activity is attributed to the presence in mdv spleen cells of a population of suppressor cells with characteristics t ... | 1978 | 659861 |
| transfer to chick embryos of incompatible dna. | after the transfer of chicken dna to chick embryos incompatible at the b locus a retardation of growth was observed. retarded differentiation of muscle fibres was histochemically detected in hatched chicks by the demonstration of enzymatic activities. the manifestations of apparent weakness of the dorsal muscles resembled a "myasthenia-like" syndrome. infection with marek's disease virus was not responsible for the damage caused by transferred dna. | 1978 | 700217 |
| effect of levamisole, an immunostimulant, on poultry experimentally inoculated with marek's disease virus. | 1978 | 700800 | |
| [intravital diagnosis of chicken infection with marek disease virus by a modified method of detecting virus-specific antigen in feather follicle epithelium]. | 1978 | 735022 | |
| marek's disease virus-induced tumor transplants: development and rejection in various genetic strains of chickens. | this study compares the ability of strains of chickens of varied genetic resistance to marek's disease (md) to resist or regress both local and general reactions to transplantable tumor cells induced by a variety of md virus strains and to transmissible lymphoid tumor cells (olson's tlt) induced by an avian leukosis-sarcoma retrovirus. previous studies have dealt with only a single md transplant. three conclusions can be drawn from our data: 1) md virus infection alone could induce progressive t ... | 1978 | 749890 |
| prevention of marek's disease: a review. | marek's disease (md) is a highly infectious neoplastic condition of chickens caused by a herpesvirus. the virus is cell associated in tumors and in all organs except in the feather follicle where enveloped infectious virions egress from the body. from this source, infection is spread horizontally by the airborne route to the environment and to other chickens. vertical transmission from dam to offspring does not occur or at best is very rare. the nonpathogenic herpesvirus of turkeys (htv) is ubiq ... | 1976 | 766959 |
| preservation of a t cell-mediated immune response in marek's disease virus-infected chickens by vaccination with a related virus. | 1976 | 774984 | |
| sequential changes in the numbers of b and t lymphocytes and other leukocytes in the blood in marek's disease. | numbers of b, t and total lymphocytes, monocytes, heterophils, eosinophils and basophils have been examined in the peripheral blood of chickens between 2 and 42 days after infection with marek's disease virus. during the stage of the acute restrictively productive virus infection of lymphoid tissues at 2-9 days after infection, absolute numbers of b cells, t cells, total lymphocytes and heterophils were increased, those of monocytes and eosinophils were decreased, and those of basophils were unc ... | 1976 | 789260 |
| effect of maternal immunity against development of marek's disease and protective ability of vaccine. | experiments were conducted to determine the susceptibility of chicks to marek's disease and the protective effect of the vaccine prepared from herpesvirus of turkey (hvt) on progeny from dams infected with marek's disease virus (mdv), or hvt, or both when they were young. chicks from dams infected with mdv or hvt-mdv were protected from the development of marek's disease when challenged at 3 days of age. the degree of protection decreased when the time of challenge was delayed; no protection was ... | 1975 | 804667 |
| morphogenesis of marek's disease virus in feather follicle epithelium. | three evaluative systems, immunodiffusion, fluorescent antibody (fa), and electron microscopy (em), were used to follow the morphogenesis of marek's disease virus in inoculated chickens. of the three, em and fa were the most sensitive in detecting early stages of infection. virus particles were found in skin biopsy specimens as early as 12 days post inoculation. immature naked particles appeared first in the nucleus; later particles were enveloped in the cytoplasm and enclosed in cytoplasmic inc ... | 1975 | 808638 |
| susceptibility of turkeys to georgia strain of marek's disease virus of chicken origin. | two experiments were conducted to study the susceptibility of turkeys to georgia strain of marek's disease virus (mdv). one-day-old chickens and turkeys were experimentally inoculated with marek's disease (md) infective plasma (experiment 1) or tumor homogenate (experiment 2) and raised in isolation for 29 weeks. the mdv inoculums were pathogenic for chickens and turkeys and caused high mortality (chickens, 100% and turkeys, 70%). macroscopic lesions of md were observed in liver, spleen, lungs, ... | 1977 | 931149 |
| detection of t-cell surface antigens in a marek's disease lymphoblastoid cell line. | indirect membrane immunofluorescence was applied in the study of a lymphoblastoid cell line derived from marek's disease lymphoma. the cell line tacked surface immunoglobulins and reacted specifically with antisera prepared against chicken thymus cells. cells transformed by marek's disease virus were of thymus origin. | 1975 | 1089795 |
| virologic and serologic studies of zoo birds for marek's disease virus infection. | one hundred and eleven zoo birds representing 49 species in 14 orders were examined for marek's disease (md) herpesvirus (mdhv) infection. mdhv was isolated from 10 birds, all belonging to genus gallus. the precipitating antibodies against mdhv were demonstrated only in the gallus birds, when 51 selected birds including 34 galliformes and 17 other birds representing 12 species from nine orders were examined. the 10 mdhv isolates all induced morphologically similar plaques in cell cultures closel ... | 1975 | 1091554 |
| viremic responses of genetically susceptible and resistant chickens to experimental infection with acute, mild, or both strains of marek's disease herpesvirus. | the development and persistence of viremia were followed in two lines of single-comb white leghorns: one experimental line (wsu-vs) highly susceptible, and one commercial line (c-wl) relatively resistant to marek's disease(md). in the resistant c-wl chicken, viremia with a mild strain of md herpesvirus (mdhv) persisted in all viremic birds through 8 weeks postinoculation (pi), while viremia with an acute strain of mdhv did not, resulting in a decrease in number of viremic birds after 2 weeks pi. ... | 1975 | 1120039 |
| a comparison of the efficacy against marek's disease of cell-free and cell-associated turkey herpesvirus vaccine. | one-day-old white leghorn and broiler chicks with maternal antibody to turkey herpesvirus (hvt) were vaccinated with 300 or 1,000 plaque-forming units (pfu) of cell-free or cell-associated hvt vaccine and challenged with virulent marek's disease virus (mdv) by contact exposure. broiler chicks receiving 300 pfu of cell-associated hvt had a 3.3% incidence of md lesions, whereas only 2.0% of those receiving 1,000 pfu had macroscopic lesions. broiler chicks vaccinated with 300 pfu of cell-free vacci ... | 1975 | 1164319 |
| efficacy of lyophilized turkey herpesvirus vaccine against marek's disease in broilers. | field trials involving approximately 1,250,000 chickens vaccinated with cell-free turkey herpesvirus (hvt) vaccine and 635,000 chickens vaccinated with cell-associated hvt vaccine howed both vaccines equally effective in reducing condemnations (attributable to marek's disease) at the poultry processing plant. vaccinated chickens were progeny of hens vaccinated at 1 day of age with cell-associated hvt vaccine. the maternal antibody did not interfere with the ability of either the cell-free or cel ... | 1975 | 1228717 |
| synthesis of immunoglobulin and marek's disease virus antibody in susceptible and relatively resistant chickens. | the levels of igm, igy, and iga and the development of specific antibody to marek's disease virus (mdv) and sheep red blood cells (srbc) in young chickens susceptible and resistant to marek's disease were compared after exposure to mdv. no significant difference was noted in the immunoglobulin levels. however, the antibody response to mdv and srbc occurred more rapidly in susceptible birds. the initial titer of antibody to these antigens was higher. these differences in response, however, were t ... | 1976 | 1255743 |
| incidence of marek's disease virus infection in domestic fowl of punjab (india). | 1976 | 1259659 | |
| effects of igy antibody on the development of marek's disease. | the effects of passive immunization with immunoglobulin y (igy) on the pathogenesis of marek's disease (md) were examined in an experimental line of white leghorn chickens highly susceptible to md. purified igy with anti-mdv antibody activity, when injected into chicks, delayed the development of mdv viremia and lesions until 9 days postinoculation (pi) with marek's disease virus (mdv). the blastogenic response of spleen cells to concanavallin-a was depressed at 6 days pi in the birds without pa ... | 1976 | 1259663 |
| characterization of reticuloendotheliosis virus-transformed avian t-lymphoblastoid cell lines infected with marek's disease virus. | the expression of marek's disease virus (mdv) transcripts and protein products was investigated in reticuloendotheliosis virus-transformed avian t-lymphoblastoid cell line recc-cu91, which was superinfected with mdv. the presence of mdv in the superinfected cell line, renamed recc-cu210, was demonstrated by southern hybridization with 32p-labeled bamhi-h and -b fragments of the bamhi mdv dna library. examination of recc-cu210 for the expression of mdv-specific rna transcripts encoded by the inte ... | 1992 | 1279200 |
| isolation and characterization of cdnas from bamhi-h gene family rnas associated with the tumorigenicity of marek's disease virus. | it has been reported that loss of the tumorigenic potential of attenuated marek's disease virus (mdv) is strongly associated with amplification of the 132-bp repeat sequences found within the bamhi-d and bamhi-h fragments contained within the long terminal repeat and the long internal repeat, respectively. the expansion of this region results in loss of transcripts that are 3.8, 3.0, and 1.8 kbp long that are produced by tumorigenic strains of mdv. this evidence suggests that production of one o ... | 1992 | 1279201 |
| sequence determination and genetic content of an 8.9-kb restriction fragment in the short unique region and the internal inverted repeat of marek's disease virus type 1 dna. | the dna sequence (8.9 kb) covering about 70% of the short unique region (us) and part of the short inverted repeat of the marek's disease virus type 1 ga strain was determined. computer analysis of the sequence showed the presence of nine potential open reading frames (orfs), consisting of more than 300 nucleotides in the us region. of these orfs, four were found to be homologous to us10 (minor virion protein), us3 (protein kinase), us2, and us6 (gd) in the us region of alpha-herpesvirus herpes ... | 1992 | 1282282 |
| isolation of chicken anaemia virus from broiler chickens. | chicken anaemia virus (cav) infection was demonstrated, by both serology and virus isolation, in 1- to 6-week-old broiler chickens originated from various parent flocks in hungary. total losses in the broiler flocks were estimated at 7 to 8% and about 25% of the chickens failed to reach target body mass by the 7th week of life. the clinical signs, postmortem lesions and histopathological changes of the affected chickens were similar to those of naturally occurring cav-induced infectious anaemia ... | 1992 | 1298167 |
| identification of a unique marek's disease virus gene which encodes a 38-kilodalton phosphoprotein and is expressed in both lytically infected cells and latently infected lymphoblastoid tumor cells. | the identification of unique marek's disease (md) virus (mdv) antigens expressed not only in lytically infected cells but also in latently infected md lymphoblastoid tumor cell lines is important in understanding the molecular mechanisms of latency and transformation by mdv, an oncogenic lymphotropic herpesvirus of chickens. through cdna and nucleotide sequence analysis, an open reading frame (designated the pp38 orf) which encodes a predicted polypeptide of 290 amino acids was identified in bam ... | 1992 | 1309266 |
| retrovirus insertion into herpesvirus in vitro and in vivo. | retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. coinfection of the same host with both these viruses is common. we report here that a retrovirus can integrate directly into a herpesvirus genome. specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (rev), into a herpesvirus, marek disease virus (mdv). both viruses are capable of inducing t lymphomas in chickens and often coexist in the same animal. rev dna integration ... | 1992 | 1310544 |
| recombinant fowlpox viruses expressing the glycoprotein b homolog and the pp38 gene of marek's disease virus. | two marek's disease virus (mdv) genes, one homologous to the glycoprotein b gene of herpes simplex virus and encoding the b antigen complex and the other encoding a 38-kda phosphorylated protein (pp38), were inserted into the fowlpox virus (fpv) genome under the control of poxvirus promoters. randomly selected nonessential regions of fpv were used for insertion, and the vaccinia virus 7.5 kda polypeptide gene promoter or a poxvirus synthetic promoter was used for expression of mdv genes. gene ex ... | 1992 | 1310754 |
| protection against marek's disease by a fowlpox virus recombinant expressing the glycoprotein b of marek's disease virus. | fowlpox virus (fpv) recombinants expressing the glycoprotein b and the phosphorylated protein (pp38) of the ga strain of marek's disease virus (mdv) were assayed for their ability to protect chickens against challenge with virulent mdv. the recombinant fpv expressing the glycoprotein b gene elicited neutralizing antibodies against mdv, significantly reduced the level of cell-associated viremia, and, similar to the conventional herpesvirus of turkeys, protected chickens against challenge with the ... | 1992 | 1310755 |
| sequence determination of cdna clones of transcripts from the tumor-associated region of the marek's disease virus genome. | the number of 132-bp tandem direct repeats within the long inverted repeat region of the marek's disease virus type 1 (mdv1) genome increases concomitantly with the loss of oncogenicity during serial passages in cultured cells. twelve clones carrying the 132-bp sequence were isolated from a cdna library constructed from chicken embryo fibroblasts infected with the mdv1 md5 strain. through sequence analysis of a cdna clone and primer extension analysis, the corresponding mrna was found to be a li ... | 1992 | 1312278 |
| investigations of environmental conditions during cluster indicate probable vectors of unknown exogenous agent(s) of multiple sclerosis. | during the tail-end of an active cluster several environmental investigations indicated that wildbirds were very probably the vectors of the unknown exogenous agent of ms. canine distemper and genetic-autoimmune theories were very definitely eliminated because of the unusual pattern of the cluster. studies of several avian pathogens unveiled marek's (mdv) and/or ibd (gumboro) as the most likely candidates for exogenous agent of ms. | 1992 | 1312422 |
| expression of the marek's disease virus (mdv) homolog of glycoprotein b of herpes simplex virus by a recombinant baculovirus and its identification as the b antigen (gp100, gp60, gp49) of mdv. | a gene encoding a homolog of glycoprotein b of herpes simplex virus (gb homolog) has been identified on the marek's disease virus (mdv) genome (l. j. n. ross, m. sanderson, s. d. scott, m. m. binns, t. doel, and b. milne, j. gen. virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of the gene product(s) are still not clear. in the present study, the gb homolog of mdv was expressed in insect cells by a recombinant baculovirus, and it was characterized to determine ... | 1992 | 1313890 |
| marek disease virus encodes a basic-leucine zipper gene resembling the fos/jun oncogenes that is highly expressed in lymphoblastoid tumors. | marek disease virus (mdv) is a herpesvirus of chickens that induces t lymphomas within 3 weeks of infection. the short latency and polyclonal nature of mdv-induced tumors have suggested that the virus may encode one or more direct-acting oncogenes. to date, however, no mdv-specific tumor antigens or candidate transforming genes have been demonstrated. in this paper, we report the identification of a mdv gene encoding a protein with homology to the leucine-zipper class of nuclear oncogenes. it al ... | 1992 | 1315048 |
| comparison of indirect immunofluorescence (if) test with enzyme-linked immunosorbent assay (elisa) in screening of hybridomas to very virulent marek's disease virus. | for identifying virus-specific antigens of marek's disease virus (mdv), monoclonal antibodies (mabs) against strain md5 of serotype 1, which is known to be a very virulent mdv (vvmdv), were isolated. fifty-eight hybridoma clones that secreted mabs against vvmdv were obtained. of these mabs, 36 gave positive reactions in an immunofluorescence (if) test, and 22 gave positive reactions on enzyme-linked immunosorbent assay (elisa). none of these mabs gave positive reactions in both the if test and e ... | 1992 | 1316535 |
| search for the origin of multiple sclerosis by first identifying the vector. | by intensive study of environmental conditions during the final stages in an active cluster area, an attempt is made to crack the enigmatic deadlock concerning the origin and etiological cause of multiple sclerosis (ms). previous investigations of other cluster areas have emphasized the probability that the outbreaks of ms were most likely caused by an unknown exogenous environmental agent, but unfortunately, the studies were conducted several years after the epidemics had occurred, making it vi ... | 1992 | 1316536 |
| detection of infectious laryngotracheitis virus infected cells with cloned dna probes. | a genomic library of infectious laryngotracheitis virus (iltv) dna bamh1 fragments was prepared and two cloned fragments were evaluated for their potential as probes for the detection of iltv infected cells. the virus was purified by a modified sucrose density gradient procedure for the isolation of pure iltv dna. a genomic library was constructed using bamh1-digested iltv dna and pgem7 as a vector. a 1.1 kb cloned bamh1 fragment of iltv dna was tested in a slot or dot blot assay for the detecti ... | 1992 | 1316798 |
| expression of the marek's disease virus homolog of herpes simplex virus glycoprotein b in escherichia coli and its identification as b antigen. | marek's disease (md) is an oncogenic disease of chickens caused by md virus (mdv). among the major glycoproteins found in mdv-infected cells are gp100, gp60, and gp49, detected by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis with antisera previously shown to be reactive with b antigen in immunodiffusion analysis. following treatment with tunicamycin (tm), an inhibitor of n-linked glycosylation, the same sera were reported to detect two molecules call ... | 1992 | 1318409 |
| suppression mediated in vitro by marek's disease virus-transformed t-lymphoblastoid cell lines: effect on lymphoproliferation. | marek's disease virus (mdv)-transformed lymphoblastoid cells (mdcc-msb1, -pa9 and -rp1) added to chicken splenic lymphocytes after treatment with mitomycin, suppress the lymphoproliferative response to t-cell mitogens (concanavalin a or phytohemagglutinin) by 40-70%. this suppressive activity was observed in syngeneic as well as in allogeneic combinations of cell lines and responder lymphocytes. the suppressive effect disappeared when the addition of md-transformed cell lines to the responder cu ... | 1992 | 1318599 |
| influence of turkey herpesvirus vaccination on the b-haplotype effect on marek's disease resistance in 15.b-congenic chickens. | eight recently developed 15.b congenic lines of chickens were tested for marek's disease (md) resistance by intra-abdominal injection of cell-associated preparations of md virus of a virulent strain (jm), a very virulent strain (md5), or md5 after vaccination with turkey herpesvirus (hvt) strain fc126. chickens of the 15.n congenic line (b15b21 or b21b21) were very resistant to jm-induced md, in contrast to chickens homozygous for the b-haplotypes 2, 5, 12, 13, 15, or 19. after md5 infection, mo ... | 1992 | 1320867 |
| complete nucleotide sequence of the marek's disease virus icp4 gene. | the marek's disease virus (mdv) gene encoding a homologue to the icp4 protein of herpes simplex virus has been mapped to bamhl fragment a based on the physical map of the mdv genome (fukuchi et al., 1984). the gene lies completely within the inverted repeat flanking the unique short region of the genome. the complete nucleotide sequence of the mdv icp4 gene has been determined. the coding region is 4245 nucleotides long and has an overall g+c content of 52%. the mdv icp4 protein is predicted to ... | 1992 | 1322594 |
| persistence of marek's disease virus in a subpopulation of b cells that is transformed by avian leukosis virus, but not in normal bursal b cells. | previous studies have described an augmentation of avian leukosis virus (alv)-induced lymphoid leukosis in chickens that were coinfected with a serotype 2 marek's disease virus (mdv) strain, sb-1. as a first step toward understanding the mechanism of this augmentation, we have analyzed the tropism of the mdv for the alv-transformed b cell. after hatching, chickens were coinfected with alv and a nonpathogenic strain of mdv, sb-1. seventy primary and metastatic alv-induced lymphomas that developed ... | 1992 | 1326647 |
| disulfide bond structure of glycoprotein d of herpes simplex virus types 1 and 2. | glycoprotein d (gd) is a structural component of the herpes simplex virus envelope which is essential for virus penetration. the function of this protein is highly dependent on its structure, and its structure is dependent on maintenance of three intact disulfide bonds. gd contains six cysteines in its ectodomain whose spacing is conserved among all its homologs in other alphaherpesviruses as well as marek's disease virus. for other proteins, conservation of cysteine spacing correlates with cons ... | 1992 | 1328685 |
| the restriction endonuclease map of marek's disease virus (mdv) serotype 2 and collinear relationship among three serotypes of mdv. | a bamhi, ecori, and xhoi restriction endonuclease map of marek's disease virus (mdv) serotype 2 (mdv2) dna was constructed by double-digest analyses of 28 cloned bamhi and 11 cloned ecori fragments of mdv2 dna, followed by hybridization tests of these cloned bamhi dna fragments with electrophoretically separated digests of mdv2-infected cell dna. on this map, mdv2 genome consisted of two segments which have unique regions inserted between two inverted repeat regions as observed in mdv serotype 1 ... | 1992 | 1329331 |
| [resistance to viral-induced tumors in the chicken]. | 1992 | 1329605 | |
| differentiation of pathogenic and non-pathogenic serotype 1 marek's disease viruses (mdvs) by the polymerase chain reaction amplification of the tandem direct repeats within the mdv genome. | there are no simple, direct methods to reliably distinguish oncogenic serotype 1 marek's disease viruses (mdvs) from their attenuated variants. the present study was an attempt to apply polymerase chain reaction (pcr) to develop a rapid and sensitive assay for the presence of the mdv genome. pcr oligos were chosen to flank the 132-base-pair tandem direct repeats in the serotype 1 mdv genome. the pcr reaction was specific for serotype 1 mdvs, amplifying fragments corresponding to one to three cop ... | 1992 | 1329706 |
| differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 by using polymerase chain reaction dna amplification. | differentiation of oncogenic and nononcogenic strains of marek's disease virus type 1 (mdv1) was attempted by polymerase chain reaction (pcr) using the primers chosen from the sequence within the long inverted repeats of mdv1 dna. pcr of the dnas extracted from oncogenic-strain-infected cells and marek's disease tumor cell lines produced a major product containing two or three copies of 132-base-pair (bp) repeat units, whereas pcrs of the dnas extracted from nononcogenic-strain-infected cells yi ... | 1992 | 1329711 |
| chicken anemia virus causes apoptosis of thymocytes after in vivo infection and of cell lines after in vitro infection. | after infection of 1-day-old chickens, chicken anemia virus (cav) causes a complete depletion of the thymic cortex by day 14. since cell death can be caused either by necrosis or by apoptosis, we investigated which type of cell death occurs after in vivo and in vitro infections with cav. using electron microscopy and biochemical methods, we demonstrated that cav induces apoptosis of cortical thymocytes after in vivo infection and of lymphoblastoid cell lines after in vitro infection. at day 13 a ... | 1992 | 1331529 |
| [gene expression of marek's disease virus--in relation to tumor induction and vaccination]. | 1992 | 1332123 | |
| marek's disease virus-transformed chicken t-cell lines respond to lymphokines. | current assays for chicken interleukin-2 (il-2) utilize mitogen-activated lymphocytes. however, very high inter-assay variability and sporadic high background proliferation limit their usefulness. in view of the above, several marek's disease virus (mdv)-transformed t-cell lines (which grow well in a serum-supplemented medium) were tested for a response to chicken il-2 when grown in serum-free media. five of six lines examined showed a dose-dependent proliferative response to chicken t-cell cond ... | 1992 | 1332253 |
| reticuloendotheliosis virus long terminal repeat elements are efficient promoters in cells of various species and tissue origin, including human lymphoid cells. | promiscuous transcriptional activity of the reticuloendotheliosis virus (rev) long terminal repeat (ltr) was detected in transient expression assays using ltr-chloramphenicol acetyltransferase-encoding gene chimeras, and cells of diverse species and tissue type; levels of expression from two different rev ltrs correlate with reports of pathogenicity of the respective viruses in vivo. revs do not encode a transactivator targeted to the viral ltr, and cells infected with marek's disease virus, a h ... | 1992 | 1332912 |
| plasmid-associated effects on test gene expression and marek's disease virus plaque formation during recombination trials. | marek's disease virus (mdv), a herpesvirus of avian origin, is being examined for suitability as a vector for expressing foreign genes. we observed that plasmids encoding the lacz gene of e. coli under the control of either the herpes simplex virus alpha 4 immediate-early promoter or the cytomegalovirus major immediate-early promoter inhibited mdv plaque formation. plaque numbers were decreased by one-third, and transient expression of the beta-galactosidase reporter gene was increased by up to ... | 1992 | 1333474 |
| antigen b of the vaccine strains of marek's disease virus and herpesvirus of turkeys presents heat-labile group and serotype specific epitopes. | antigen b of marek's disease virus (mdv) vaccine strains cvi988 and sb1 (serotypes 1 and 2) and herpesvirus of turkeys (hvt) (serotype 3) is formed of oligomeric molecules that are detergent-stable and heat-labile. immunoblots of native membranal extracts of hvt- and mdv-infected chick embryo fibroblasts (cef) probed with avian monoserotypic antisera, murine monoclonal antibodies (mab) to the three serotypes and mab to antigen b showed two distinct patterns of high molecular weight oligomeric an ... | 1992 | 1333751 |
| polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 marek's disease viruses (mdv) and vaccine viruses of mdv-serotypes 2 and 3. | a polymerase chain reaction (pcr) test based on primers flanking the 132 bp tandem repeat in pathogenic mdv-1 dna was developed. these primers amplify a dimer or a trimer 132 bp repeat in pathogenic mdv-1 dna from blood and organs of commercial chickens with marek's disease (md) symptoms. using the same primers in a radioactive pcr test, it was possible to distinguish between vvmdv-1 and the non-pathogenic mdv-1 cvi-988 vaccine in which the 132 bp repeats in the dna were increased up to 9 repeat ... | 1992 | 1335457 |
| use of a soluble tetrazolium/formazan assay for chicken cells. | we evaluated a soluble tetrazolium/formazan assay using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)-carbonyl ]-2h- tetrazolium hydroxide (xtt) for chicken cell growth. fifty microliter of solution containing 1 mg/ml of xtt and 0.025 mm phenazine methosulfate was added to the cells in a well of 96-well microplate. after 4 hr incubation at 37 degrees c, the absorbance was measured at 490 nm. under this condition, absorbances were well correlated with cell number of marek's disease tu ... | 1992 | 1335760 |
| cell-mediated cytolysis of lymphoblastoid cells expressing marek's disease virus-specific phosphorylated polypeptides. | cell-mediated immune responses against marek's disease virus (mdv)-antigens were examined using reticuloendotheliosis virus (rev)-transformed lymphoblastoid cell line cu91 and three cell lines derived from cu91. cu210 was established by establishing a latent mdv infection in cu91. transfection of cu210 with pnl1, a selectable plasmid or with pnl1 and the cloned bamhi a fragment of mdv dna resulted in the establishment of cu212 and cu211, respectively. cu211 expressed a mdv-specific phosphorylate ... | 1992 | 1336254 |
| [isolation and study of biological properties of non-oncogenic marek's disease herpesviruses in chickens. 1. characterization in vitro]. | the present paper deals with isolation of nonpathogenic chicken herpesviruses of marek's disease (md) and their in vitro characterization. two strains of herpesviruses (denoted as vub-m and -k isolates) were isolated from leucocytes separated from the blood of fattening chickens without clinical and pathomorphological symptoms of marek's disease and from the blood of laying hens of a laboratory nonvaccinated flock without clinical symptoms, but with almost 100% incidence of precipitating md anti ... | 1992 | 1337955 |
| [isolation and study of biological properties of non-oncogenic marek's disease herpesviruses in chickens. 2. characterization in vivo]. | in a previous paper (jurajda and halouzka, 1992) the in vitro isolation of two chicken herpesviruses of marek's disease (m and k strains) was described and results of their characterization were presented. the present paper deals with the in vivo characterization of both isolates: pathogenicity and immunosuppressive characteristics of isolates were observed in a five-week test period, along with the development and production dynamics of antibodies and viral antigen in the feathers of experiment ... | 1992 | 1337956 |
| marek's disease virus-mediated enhancement of avian leukosis virus gene expression and virus production. | direct interaction between two viruses in coinfected cells may promote replication and pathogenesis of one or both virus types. synergism between herpesviruses and retroviruses is an important factor in diagnosis, treatment, and prevention of animal and human diseases. in birds, marek's disease virus (mdv) may be an important cofactor in avian leukosis virus induced disease. infection of susceptible cells with non-oncogenic serotype 2 mdv, an avian herpesvirus, and rous-associated virus type 2 ( ... | 1992 | 1370125 |
| protection of chickens from newcastle and marek's diseases with a recombinant herpesvirus of turkeys vaccine expressing the newcastle disease virus fusion protein. | recombinant strains of herpesvirus of turkeys (hvt) were constructed that contain either the fusion protein gene or the hemagglutinin-neuraminidase gene of newcastle disease virus (ndv) inserted into a nonessential gene of hvt. expression of the ndv antigens was regulated from a strong promoter element derived from the rous sarcoma virus long terminal repeat. recombinant hvt strains were stable and fully infectious in cell culture and in chickens. chickens receiving a single intra-abdominal inoc ... | 1992 | 1485872 |
| immunopathology of marek's disease in quails: presence of antinuclear antibody and immune complex. | antinuclear antibody (ana), a marker for autoimmune reactions, was detected in the sera of quails with marek's disease (md). the autoantibody was detected 3 weeks after infection in quails infected with chicken marek's disease virus and 4 weeks after infection in quails infected with quail marek's disease virus. the ana titers were low and ranged from 10 to 40. a speckled type of nuclear fluorescence was the characteristic staining feature. in addition to the presence of ana, immune complexes (i ... | 1991 | 1647066 |
| importance of cell-mediated immunity in marek's disease and other viral tumor diseases. | cell-mediated immune (cmi) responses to viral tumor diseases are often used as examples of the importance of antiviral and antitumor immunity in chickens. especially, reticuloendotheliosis virus (rev) and marek's disease herpesvirus (mdv) are used as models to study the development of cytotoxic t-lymphocytes against viral and tumor antigens and activation of natural killer (nk) cells. major histocompatibility complex class i-restricted, antiviral cytotoxic t-lymphocytes expressing cd4-/cd8+ mark ... | 1991 | 1649464 |
| the early pathogenesis in chicken inoculated with non-pathogenic serotype 2 marek's disease virus. | a newly cloned serotype 2 marek's disease virus (mdv), strain ml-6, was inoculated via the nasal cavity in specific-pathogen-free chicks to examine early virus replication and the expression of marek's disease (md)-related antigens. following inoculation, viral intracellular antigens (vias) were detected in lymphoid organs (bursas and spleens) between 5 and 14 days post inoculation (pi), in feather follicles between 14 and 30 days pi, and in lungs at 3 days pi by the immunohistopathological stai ... | 1991 | 1650601 |
| cloning, sequencing, and functional analysis of a marek's disease virus origin of dna replication. | previously, we isolated a replicon from a defective marek's disease virus (mdv), analogous to defective herpes simplex viruses (amplicons). defective viruses contain cis-acting elements required for dna synthesis and virus propagation such as an origin of dna replication and a packaging-cleavage signal site. in this report, the mdv replicon was utilized to locate an origin of mdv dna replication. a comparison of mdv replicon sequences with other herpesvirus replication origin sequences revealed ... | 1991 | 1656099 |
| identification of a novel transcription factor, acf, in cultured avian fibroblast cells that interacts with a marek's disease virus late gene promoter. | interactions between factors in duck and chick embryo fibroblast (def and cef, respectively) nuclear extracts and the marek's disease virus (mdv) gp57-65 gene promoter were investigated. results of in vitro transcription and gel mobility-shift assays indicated that multiple cellular factors interact with 5'-flanking sequences of the mdv gp57-65 gene. one sequence-specific dna binding activity (termed acf for avian cell factor(s)) was identified by interaction of def and cef nuclear extract prote ... | 1991 | 1656607 |
| structural analysis and transcriptional mapping of the marek's disease virus gene encoding pp38, an antigen associated with transformed cells. | the gene encoding a marek's disease virus (mdv) pp38 phosphoprotein has been identified, sequenced, and localized to the bamhi h fragment to the left of the putative mdv origin of replication. the open reading frame was defined by sequencing of a lacz-pp38 fusion protein gene from a lambda gt11 expression library. the entire open reading frame is 290 amino acids long and codes for a protein with a calculated molecular weight of 31,169, compared with the size of 38 kda of the phosphorylated form ... | 1991 | 1658357 |
| antibody directed against marek's disease-associated tumor surface antigen can be eluted from marek's disease tumor cells. | antibody directed against marek's disease-associated tumor surface antigen (matsa) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from marek's disease virus-infected chickens. feather follicular marek's disease virus (mdv) antigen could not be demonstrated with this antibody by indirect immunofluorescent (if) staining. monoclonal antibody directed against matsa could completely block the activity of eluted antibody and vice versa. by indirect if stai ... | 1991 | 1659032 |
| humoral immune responses to inactivated oil-emulsified marek's disease vaccine. | when inactivated md11/75c vaccine was inoculated into 1-day-old chickens, it stimulated antibodies detectable by enzyme-linked immunosorbent assay (at a titer of 6400) and indirect fluorescent antibody test (at a titer of 640), but lacking virus-neutralizing activity. chickens passively inoculated with these antibodies were protected against bursal atrophy, weight loss, and early mortality when challenged with the virulent md5 strain of marek's disease virus (mdv). that led to the conclusion tha ... | 1991 | 1659363 |
| restriction endonuclease analysis of marek's disease virus dna: differentiation of viral strains and determination of passage history. | the restriction endonuclease (re) patterns of dna from serotype 1 marek's disease viruses (mdvs) are unique to serotype 1 viruses and can also be used to differentiate between low and high cell-culture-passaged viruses. we compared the re patterns of dna from seven serotype 2 and 3 mdvs before and after serial in vitro passage. passage of four serotype 2 strains resulted in a variety of changes in the re pattern. individual strains within a serotype exhibited unique restriction patterns that all ... | 1991 | 1659367 |
| purification and characterization of infectious marek's disease virus genomes using pulsed field electrophoresis. | marek's disease virus (mdv) is an acutely oncogenic avian herpesvirus. the tightly cell-associated in vitro growth characteristics of mdv present unique problems when attempting to purify, analyze, and manipulate mdv genomes. to facilitate molecular characterization of mdv, contour-clamped homogeneous electric fields electrophoresis (chef) was used to purify infectious mdv genomes. chef techniques were optimized for evaluation of total genome size and alterations in structure which occur during ... | 1991 | 1660199 |
| nucleosomal structure of marek's disease virus genome in transformed lymphoblastoid cell lines, mdcc-msb1 and mdcc-rp1. | the latent mdv (marek's disease virus) genomes are folded into nucleosomal structures in both virus-productive and -nonproductive lymphoblastoid cell lines, mdcc-msb1 (msb-1) and -rp1 (rp-1), respectively. there was no difference between transcriptionally active and inactive regions of mdv genome with regard to nucleosomal patterns. in order to investigate whether nucleosomal structure is correlated with the repression of the transcription of mdv genome in lymphoblastoid cells, we examined the d ... | 1991 | 1661363 |
| monospecific antibodies to marek's disease virus antigen b dimer (200 kda) and monomer (130 and 60 kda) glycoproteins neutralize virus infectivity and detect the antigen b proteins in infected cell membranes. | monospecific antibodies were prepared by nitrocellulose blot immunoaffinity to 3 polypeptide components of the host-membrane associated b antigen of marek's disease herpesvirus (mdv) and to its soluble a antigen. the b antigen comprised a 200 kda dimer which is 2-mercaptoethanol (2-me) labile, a monomer of 130 kda and a 60 kda protein, both of which are 2-me resistant. cross-immunoblotting studies showed that the anti-dimer antibody recognized the dimer protein as well as the 130 and 60 kda comp ... | 1991 | 1662035 |
| proliferation of chicken lymphoblastoid cells after in vitro infection with marek's disease virus. | activated, thymus-derived (t) lymphoblasts were exposed to marek's disease virus and cultivated in attempts to induce in vitro transformation. after 9 to 15 days, colonies or small clusters of proliferating lymphoblasts were observed in cultures from three of a total of 122 attempts. these developed into proliferating cell cultures that resembled conventional marek's disease (md) lymphoblastoid cell lines in terms of growth characteristics and morphology. all proliferative cultures were unusual ... | 1991 | 1664718 |
| attenuated revertant serotype 1 marek's disease viruses: safety and protective efficacy. | in earlier studies, a revertant serotype 1 marek's disease virus (mdv), clone md11/75c/r2, was found to be a highly protective vaccine virus but was mildly pathogenic for susceptible chickens. the term "revertant" indicates that the virus, after attenuation, gained virulence following backpassage in chickens. the present study is an attempt to develop a more attenuated but still protective vaccine virus from md11/75c/r2. forty-two derivative viruses or clones from md11/75c/r2 were evaluated. two ... | 1991 | 1664721 |
| deletion within the cyp17 gene together with insertion of foreign dna is the cause of combined complete 17 alpha-hydroxylase/17,20-lyase deficiency in an italian patient. | the molecular basis of 17 alpha-hydroxylase/17,20-lyase deficiency syndrome in a 14-yr-old 46,xy italian patient was investigated by amplification, subcloning, and sequencing of specific exonic sequences from genomic dna samples. a homozygous mutation, consisting of a 518-basepair (bp) deletion combined with a 469-bp insertion, was identified in the cyp17 gene of the patient. the deletion spans much of exon ii, the whole intron 2, and a portion of exon iii. a part (156 bp) of the inserted sequen ... | 1991 | 1665206 |
| vaccination against herpesvirus, fiction or reality? | against herpes simplex type 1 (hsv-1) there are commercially available vaccines which may induce protection against symptoms of primary infections, but not against establishment of latency. effective animal vaccines have been developed against herpes simplex-like viruses, for example pseudorabies vaccine for pigs, and an experimental vaccine can protect cattle against infection with infectious bovine rhinotracheitis (ibr) virus. there are also commercially available effective live vaccines again ... | 1991 | 1666442 |
| marek's disease virus gene clones encoding virus-specific phosphorylated polypeptides and serological characterization of fusion proteins. | marek's disease virus (mdv) gene clones, ra2 and ga8, constructed in e. coli bacteriophage lambda-gt11 (gt11) were identified by a monoclonal antibody (mab), h19.47, against a putative transformation-related viral antigen consisting of a complex of three phosphorylated polypeptides, pp41, pp38, and pp24. both recombinants have a mdv-dna insert of about 0.5 kb and are mapped to the region of bamhi-h or ecori-x fragments of the mdv genome by southern blot hybridization. immunoblot and immunoprecip ... | 1990 | 1693456 |
| latent transcripts of marek's disease virus are clustered in the short and long repeat regions. | marek's disease herpesvirus (mdv) induces tumors in chickens, and lymphoblastoid cells derived from such tumors contain the viral genome in a latent state and do not produce infectious virus. poly(a)+ rnas extracted from mdv-induced kidney lymphoma cells and from mkt-1 cells, a nonproducing lymphoblastoid cell line derived from a tumor induced by mdv, were electrophoretically separated under denaturing conditions, transferred to a solid substrate, and hybridized with labeled dna probes represent ... | 1990 | 1700823 |
| analysis of marek's disease tumor-associated surface antigen on mdcc-msb1-clo.18 cells. | the physico-chemical nature of marek's disease tumor-associated surface antigen (matsa) on marek's disease (md) lymphoblastoid tumor cell line (mdcc-msb1-clo.18) was examined by the cellular enzyme-linked immunosorbent assay (celisa) and the sandwich enzyme-linked immunosorbent assay (elisa) using an anti-matsa immune serum or a monoclonal antibody (mab) 2b9 developed against matsa. our results indicate that matsa is a glycoprotein and 2b9 recognizes an antigenic site in the protein moiety of ma ... | 1991 | 1703127 |
| identification of viral proteins encoded by two dna fragments of herpesvirus of turkeys (hvt). | herpesvirus of turkeys (hvt) vaccine is used worldwide to immunize chickens against marek's disease (md). polyclonal antiserum directed against one virus cross-reacts with proteins of the other, while only 5% homology at the dna level was demonstrated between the two viruses. a partial library of hvt dna fragments ranging from 1.5 to 13.5 kbp in size was constructed in pbr 322. under stringent conditions of hybridization (low salt concentration, 10% dextran sulfate at 68 degrees c), two of the c ... | 1991 | 1722930 |
| transformation of t-lymphocyte subsets by marek's disease herpesvirus. | marek's disease herpesvirus (mdv)-transformed lymphoblastoid tumor cell lines were characterized for the presence of the surface markers. monoclonal antibodies were used for cd3 (t-cell receptor [tcr] complex), tcr1, tcr2, and tcr3, cd4, cd8, and ia antigen by indirect fluorescence staining followed by microscopic examination or flow cytometry. the lymphoblastoid cell lines were obtained from tumors from chickens infected with mdv (n = 44) or from local lesions induced by inoculation of allogene ... | 1991 | 1847460 |
| isolation of a marek's disease virus (mdv) recombinant containing the lacz gene of escherichia coli stably inserted within the mdv us2 gene. | we have isolated a stable, recombinant marek's disease virus (mdv) containing the lacz gene of escherichia coli inserted into the unique short region of the genome. the nucleotide sequence of the insertion site indicates that it lies within a sequence homologous to the us2 gene of herpes simplex virus. stable insertion of the lacz gene into the mdv us2 gene indicates that the site is nonessential for mdv growth in cell culture. | 1991 | 1847475 |
| properties and evolutionary relationships of the marek's disease virus homologues of protein kinase, glycoprotein d and glycoprotein i of herpes simplex virus. | the deduced amino acid sequences of the open reading frames (orfs) mapping in the short unique segment (us) of marek's disease virus (mdv) reported in the accompanying paper have been analysed using computer programs to determine their relationships to herpesvirus proteins. analysis of the catalytic domains of protein kinases showed that the mdv kinase (mdv pk) was closely related to the alphaherpesvirus protein kinase mapping in us. the results also showed that the mdv pk was more closely relat ... | 1991 | 1849976 |
| dna sequence and organization of genes in a 5.5 kbp ecori fragment mapping in the short unique segment of marek's disease virus (strain rb1b). | the dna sequence of a 5.5 kbp ecori fragment located in the short unique region (us) of the 'highly oncogenic' strain rb1b of marek's disease virus (mdv) was determined. the sequence contained six open reading frames (orfs), four of which were homologous to proteins mapping in the us region of herpes simplex virus type 1 (hsv-1). these include the homologues of hsv-1 protein kinase, glycoprotein d (gd), glycoprotein i (gi) and us2 which is of unknown function. the mdv orfs had a marked bias for ... | 1991 | 1849977 |
| multiple bidirectional initiations and terminations of transcription in the marek's disease virus long repeat regions. | marek's disease is an oncogenic disease of chickens caused by a herpesvirus, marek's disease virus (mdv). serial in vitro passage of pathogenic mdv results in amplification of a 132-bp direct repeat in the mdv genome's trl and irl repeat regions and loss of tumorigenicity. this led to the hypothesis that upon such expansion, one or more tumor-inducing genes fail to be expressed. in this report a group of cdnas mapping in the expanded regions were isolated from a pathogenic mdv strain in which th ... | 1991 | 1850022 |
| efficacy of coarse-spray administration of a reovirus vaccine in young chickens. | coarse-spray (cs) administration of a commercial s1133 reovirus vaccine in chickens for prevention of clinical viral tenosynovitis (vt) infection was evaluated. in expt. 1, one-day-old specific-pathogen-free (spf) white leghorns were vaccinated with a combination of reovirus, newcastle disease (nd), and infectious bronchitis (ib) vaccines by cs and infectious bursal disease vaccine by the subcutaneous (sq) route. in expt. 2, one-day-old commercial broilers were vaccinated by cs with reovirus vac ... | 1991 | 1851416 |
| inhibition of marek's disease virus dna transfection by a sequence containing an alphaherpesvirus origin of replication and flanking transcriptional regulatory elements. | a co-transfection method was used to identify regions of the marek's disease virus (mdv) genome that inhibit plaque formation when introduced along with total dna from mdv-infected chicken embryo fibroblasts into secondary chicken embryo fibroblasts. co-transfections involving mdv plasmids containing the bamhi-d or bamhi-h regions of the genome inhibited plaque formation more than 10-fold. the inhibitory region was localized to a 222-base-pair region that contains a sequence homologous to the co ... | 1991 | 1851421 |
| expression of the a antigen (gp57-65) of marek's disease virus by a recombinant baculovirus. | a recombinant baculovirus expressing the a antigen (a ag) of marek's disease virus (mdv) was constructed. in spodoptera fruiperda (sf) cells infected with the recombinant virus, a ag expression was localized to the cell surface. only a small amount of recombinant a ag was detected in the culture supernatant of infected sf cells, but authentic a ag is mainly secreted into the culture supernatant of mdv-infected chicken embryo fibroblasts. cell surface-associated recombinant a ag seemed to be slig ... | 1991 | 1851809 |
| the inhibitory effects of oligonucleotides, complementary to marek's disease virus mrna transcribed from the bamhi-h region, on the proliferation of transformed lymphoblastoid cells, mdcc-msb1. | an oligonucleotide complementary to the splice donor sequence of the 1.8 kb gene family produced from the bamhi-h region of marek's disease virus (mdv) dna inhibited the proliferation of the mdv-derived lymphoblastoid cell line, mdcc-msb1 (msb-1), but not that of the avian lymphoid leukosis-derived lymphoblastoid cell line, lscc-bk3. colony formation in soft agar was also inhibited by treatment of msb-1 cells with the antisense oligonucleotide. it is hypothesized that expression of the 1.8 kb ge ... | 1991 | 1851810 |
| inverted repeat regions of marek's disease virus dna possess a structure similar to that of the a sequence of herpes simplex virus dna and contain host cell telomere sequences. | the genomic structure of marek's disease virus (mdv) is similar to those of the alphaherpesviruses herpes simplex virus (hsv) types 1 and 2. sequence analysis of the junction region between the long component (l) and the short component (s) revealed the existence of an a-like sequence, similar in structure to the a sequence of hsv-1. further study revealed that the mdv genome contains five copies of the a-like sequence within the long terminal repeat region as well as in the short terminal repea ... | 1991 | 1851854 |
| transforming potential is detectable in arteriosclerotic plaques of young animals. | the carcinogen-treated cockerel is a model for studying the early stages of arteriosclerotic plaque development. carcinogen administration accelerates arteriosclerotic plaque development in cockerels, and transforming elements are present in dna from advanced human plaques. in this study, we asked whether transforming elements could also be detected at early stages of plaque development in cockerels. nih3t3 cells were transfected with dna from plaques isolated from carcinogen-treated cockerels a ... | 1991 | 1905951 |
| effect of aflatoxin b1 on the efficacy of turkey herpesvirus vaccine against marek's disease. | the effect of feeding aflatoxin b1 (afb1) (0.5 ppm) was studied in young chicks. the frequency and the severity of gross and microscopic lesions of marek's disease were significantly higher in those birds which had been vaccinated with turkey herpesvirus (hvi) and birds challenged with marek's disease virus which had been given afb1 in the feed than in those given normal feed. the protective efficacy of hvt vaccine, as judged on the basis of gross and histopathological lesions, was 86.1 and 77.3 ... | 1991 | 1910199 |