Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a tentative transmission mechanism for the twitch elicited in guinea-pig vas deferens by field stimulation. | the twitch response to nerve activity in the vas deferens does not behave as if it were adrenergic since it is enhanced by adrenoceptor blockers and often inhibited by noradrenaline and other sympathomimieic amines. potassium (k+) ions have a strong reinforcing action on the twitch and easily restore it after blockade by lanthanum. the hypothesis is advanced that k+ ions have a transmitter function in the vas deferens and that k+ ions released in conjunction with the nerve stimulus may reach a c ... | 1975 | 6863 |
| letter: sedaperone and hypnodil as anesthesia in cesarean section in swine. | 1976 | 6941 | |
| observations on ouabain binding and membrane phosphorylation by the sodium pump. | 1976 | 6964 | |
| mechanisms and regulation of biosynthesis of saturated fatty acids. | 1976 | 6981 | |
| prostaglandin ix-synthesis of (+/-)-15-methyl-11-deoxy pge1 (doxaprost) - a potent bronchodilator - and its c-15-epimer. | 1976 | 7000 | |
| japanese encephalitis virus from pigs and mosquitoes in jakarta, indonesia. | japanese encephalitis virus was isolated from 11 pools of culex tritaeniorhynchus and a single pool of c. gelidus mosquitoes at a pig-raising area near jakarta, west java, indonesia, during 1972-74. ten sentinel pigs placed in the area all developed haemagglutination-inhibition antibodies against japanese encephalitis and the virus was isolated from the blood of 3. | 1975 | 7033 |
| effect of atropine sulfate, papaverin hc1 and antihistaminic preparations on isolated organs of various experimental animals. | 1975 | 7039 | |
| values of acid-base equilibrium in the blood of various species of domestic animals. | by means of the astrup equilibration method the values of the acid-base balance of the blood were determined in 104 cows, 99 horses, 100 pigs, 15 sheep, 20 goats, and in 101 dogs. the ph values of the blood, the partial pressure of co2, the base excess, the base buffer, the standard bicarbonate, the actual bicarbonate, and the total co2 were processed statistically and are presented in tables. | 1976 | 7040 |
| species and phenobarbitone-induced differences in the kinetic constants of liver microsomal harmine o-demethylation. | 1. the apparent kinetic constants for the o-demethylation of harmine to harmol by 10 000 g supernatant fractions from livers of mice, rats, guinea-pigs, rabbits, cats and cows have been determined. the km values were 10-39 mum and vmax 0-25 and 1-65 nmol/mg protein/min. 2. optimal conditions of incubation time and nadp requirements differed between species. in all species except cat and cow the rate of o-demethylation of harmine was linear for 5 min, but in the latter species was linear for 15 m ... | 1976 | 7043 |
| results of a study of the protective and preventive properties of pms-400 cream with universal protective properties. | 1975 | 7054 | |
| search for persistent epizootic venezuelan encephalitis virus in guatemala, el salvador and nicaragua during 1970-1975. | evidence was sought during 1970-1975 of persistence of equine-virulent venezuelan encephalitis (ve) virus in regions of central america that were heavily involved in the epidemic-equine epizootic of 1969. (a) four sentinel horses were exposed in an arid, upland region of the atlantic drainage of guatemala during august-october 1970, but no horse became infected. (b) the epicenter region of the 1969 outbreak, in southwestern guatemala and southwestern el salvador, was studied during july 1970-feb ... | 1976 | 7139 |
| uric acid excretion by the pig kidney. | the handling of uric acid by the pig kidney has been investigated during continuous urate infusion in unrestrained, unanesthetized animals. urate-to-inulin clearance rates in excess of 1 were found under all experimental conditions, demonstrating only net secretion by the pig kidney. the demonstration of a secretory maximum was precluded owing to a progressive reduction in the gfr associated with high rates of urate infusion. urate clearance was independent of urine flow rate up to 10 ml/min. th ... | 1976 | 7142 |
| sympathoadrenal neurochemistry and early weaning of swine. | three litters of pigs were weaned at 21 days of age, and 3 others were left with the sow. pigs were killed at 21, 23, 28, or 39 days of age. weaned pigs exhibited anxiety, gastrointestinal dysfunction, and decreased rate of body weight gain. plasma glucose or liver glycogen concentrations were not decreased by weaning. adrenal gland weights and tyrosine hydroxylase (ec 1.14.3a), dopamine beta-hydroxylase (ec 1.14.2.1), phenethanolamine-n-methyl transferase (ec 2.1.1), and monoamine oxidase (ec 1 ... | 1976 | 7171 |
| behavioral pharmacology and toxicology. | 1976 | 7185 | |
| toxicology of inhalation anesthetics and metabolites. | 1976 | 7186 | |
| factors affecting morphine uptake into kidney slices. | 1976 | 7262 | |
| d-aspartate oxidase from octopus liver (octopus vulgaris lam.) and from pig liver. purification and properties. iii. comparative study of the d-aspartate oxidase purified from octopus liver and swine liver. | 1976 | 7282 | |
| gastric secretion and fermentation in the suckling pig. | 1. the contribution to acidification of the stomach contents of pigs by hydrochloric acid secretion or by lactic acid produced by fermentation was studied in fifteen suckling pigs from six litters born and reared either in a 'conventional' environment or in an isolated 'clean' environment. sequential samples of stomach contents obtained during periods of up to 24 h were analysed for their chloride and lactic acid contents, ph and total titratable acidity. these values gave a measure of organic a ... | 1976 | 7286 |
| purification and properties of two lipases from pig adipose tissue. | two lipases were purified from pig adipose tissue after delipidation by a mild and effective procedure using mixtures of chloroform and butanol. this was followed by hydrophobic adsorption chromatography on aminohexyl-sepharose 4b coupled with octanoic acid, gel filtration on sephadex g-100, and isoelectric focusing. two electrophoretically and chromatographically pure enzymes were obtained, which had the same molecular weight (60 000 +/- 3000) and specific activity, and almost identical amino a ... | 1976 | 7300 |
| histamine (h2) receptor-adenylate cyclase system in pig skin (epidermis). | histamine activated adenylate cyclase in pig skin (epidermal) slices, resulting in the accumulation of cyclic amp. this effect was highly potentiated by the addition of cyclic amp-phosphodiesterase inhibitors (theophylline, papaverine). a specific h2 receptor inhibitor (metiamide) inhibited the effect of histamine completely, while other antihistamines (diphenhydramine, acetophenazine, perphenazine, fluphenazine, promethazine) inhibited the effect of histamine to various lesser degrees. it has b ... | 1976 | 7311 |
| oxidation of sarcosine and n-alkyl derivatives of glycine by d-amino-acid oxidase. | 1. sarcosine was oxidized by d-amino-acid oxidase (d-amino-acid: o2 oxidoreductase (deaminating), ec 1.4.3.3) to yield methylamine and glyoxylic acid. a seriies of n-alkyl glycines were also oxidized by this enzyme. 2. n-acetyl- and n-phenylglycine inhibited the oxidase by competing with the substrate, while n-methyl-n-acetylglycine did not bind to the enzyme. this suggests the requirement of at least one unsubstituted hydrogen atom at the amino group ofglycine for binding. 3. the primary step i ... | 1976 | 7319 |
| blockade by burimamide of the restorative effect of histamine in tetrodotoxin-treated heart preparations. | 1 in isolated heart preparations in which fast sodium channels were blocked by tetrodotoxin (2-4 x 10(-5) m), excitability was restored by histamine (6 x 10(-6) m to 10(-5) m). 2 this effect was antagonized by edta (2 x 10(-6) m),d600 compound (0.5mug/ml) and the h2-receptor antagonist, burimamide(2 x 10(-4) m). | 1976 | 7335 |
| models for depolymerizing enzymes: criteria for discrimination of models. | several models for the action of alpha amylase have been proposed to account for the nonrandom distribution of oligosaccharides in the amylase digests of polysaccharides. the preferred-attack model attempts to account for the nonrandom distribution by assuming that the probability for bond cleavage depends upon the position of the bond in the chain. the repetitive, or multiple-attack, model suggests that the nonrandom distribution of oligosaccharides arises because an amylase can form a cage-lik ... | 1976 | 7353 |
| simultaneous identification of t and b cells in blood smears using antibody coated bacteria. | 1976 | 7360 | |
| adenylyl cyclase activities in ovarian tissues. i. homogenization and conditions of assay in graafian follicles and corpora lutea of rabbits, rats, and pigs: regulation by atp, and some comparative properties. | responsiveness of ovarian adenylyl cyclases to luteinizing hormone (lh), found to be 5 to 10-fold in cell-free preparations under optimal conditions, required gentle homogenizations and storage in sucrose-containing media. assay conditions required the use of an atp-regenerating system consisting of creatine kinase, creatine phosphate, and myokinase for the preservation of atp levels. lh-stimulated adenylyl cyclase (ac) in rabbit cl showed the following properties: 1) the ph optimum of basal act ... | 1976 | 7447 |
| a new type of fluorocarbon liquid oxygenator. | a new oxygenator based on gas transfer across liquid-liquid interfaces (liquid oxygenator) is introduced. perfluorinated chemicals are used as gas carriers. this liquid oxygenator differs from others in its concept basing on the dispersion of the blood droplets in an inert liquid by means of shear forces. this method allows high efficiency both with respect to saturation and to required blood flow. the results of gas transfer experiments in vitro and in vivo are satisfactory. | 1976 | 7460 |
| a simplified bloodless procedure for extensive hepatectomy. experimental study in the pig. | an original procedure of extensive bloodless hepatectomy is described in the pig. complete vascular liver isolation with aorta clamping up to 25 min allows 60-75% bloodless hepatectomy without any portal triad dissection. perfect hemostasis and shortening of liver ischemia is obtained with the use of a gelatin-resorcin-formaldehyde adhesive. hemodynamic tolerance is good, and liver regeneration appears to be as fast as in minor species. | 1976 | 7461 |
| salt effect on diamine oxidase activity. | 1976 | 7538 | |
| proceedings: comparative aspects of glyceraldehyde-3-phosphate dehydrogenases from mammalian and green plant tissues. | 1976 | 7539 | |
| purification and properties of cathepsin d from porcine spleen. | cathepsin d was purified from porcine spleen to near homogeneity as determined by gel electrophoresis. the isolation scheme involved an acid precipitation of tissue extract, deae-cellulose and sephadex g-200 chromatography, and isoelectric focusing. the end product represented about a 1000-fold purification and about a 10% recovery. the purified enzyme was the major isoenzyme, which represented 60% of cathepsin d present in porcine spleen. two minor isoenzymes of cathepsin d were present in smal ... | 1976 | 7560 |
| requirement of an essential thiol group and ferric iron for the activity of the progesterone-induced porcine uterine purple phosphatase. | the progesterone-induced purple phosphatase isolated from the uterine flushings of pigs is activated by a variety of reagents that cleave disulfide bonds, including 2-mercaptoethanol, dithiothreitol, l-ascorbate, l-cysteine, sulfite, and cyanide. it is inhibited by various mercurials, iodoacetamide, o-iodosobenzoate, and hydrogen peroxide. thiols increase the specific phosphatase activity from 25 to about 300 units per mg of enzyme. this activation is accompanied by a shift in the extinction max ... | 1976 | 7564 |
| synthetic peptides for chymosin and pepsin assays: ph effect and pepsin independent-determination in mixtures. | peptide i h-phe-gly-his-phe(no2)-phe-ala-phe-ome hydrolyzed by chymosin with kcat=.3+/-.3 s-1 and km=7+/-3 mm (ph 4.7) inhibited competitively peptide ii h-leu-ser-phe(no2)-nle-ala-leu-ome hydrolysis by chymosin with ki=.23 +/- .12 mm at ph 4.7. in reference conditions (.4 mm peptide, .01 m acetate buffer ph 4.7), the specific activities of porcine pepsin and chymosin on peptide i were 470 +/- 70 nm s-1 and .8 nm s-1 per mg of enzyme. this difference in specific activity for peptide i allowed de ... | 1976 | 7580 |
| the pharmacology of flazalone: a new class of anti-inflammatory agent. | the anti-inflammatory activity of flazalone, a unique chemical drug, is described. in acute irritant anti-inflammatory tests, flazalone exhibited a wide spectrum of activity. the compound was active in affecting the course of paw swelling in adjuvant arthritis when given daily either at the outset of the polyarthritis or after induction. the most unusual aspect of this compound is its ability to inhibit graft rejection in goldfish and rabbits. the pattern of anti-inflammatory activity does not a ... | 1976 | 7668 |
| potential histamine h2-receptor antagonists. 3. methylhistamines. | syntheses are described for all the mono- and some di- and trimethylhistamines. new methods are given for the known npi, ntau-, nalpha-, 2-, and 4-methylhistamines and for the novel compounds, beta-methyl-, 4,nalpha-dimethyl-, and 4,nalpha,nalpha-trimethylhistamines. agonist activities are reported for stimulation of histamine h1 (guinea-pig ileum) and h2 (rat gastric acid secretion) receptors. h2-receptor agonist activities indicate that a methyl group is more readily accommodated at the 4 and ... | 1976 | 7675 |
| studies on the structure activity relationship of adrenergic beta-mimetic benzylamine derivatives. | appropriately substituted benzylamine (bza) derivatives, fragmented derivatives of tetrahydroisoquinolines, were found to be directly acting adrenergic beta-stimulants, exhibiting tracheal relaxing, positive chronotropic and free fatty acid (ffa) releasing activities. the chemical structures essential for manifestation of the beta-action were i) 3,4-dihydroxybenzylamine, ii) arylmethyl group at position alpha, iii) lower alkyl group on the n atom. the structure activity relationships of bza-deri ... | 1976 | 7693 |
| the mechanism of action of d-amino acid oxidase. i. evidence for a free radical mechanism of the reaction catalyzed b a dimeric form of the enzyme. | d-amino acid oxidase can oxidize the substrate to a ketoacid in the absence of oxygen. the stoichiometry of this reaction is precisely 1 molecule of keto acid for 1 molecule of enzyme, containing two flavin groups. hence, the flavin must be in the semi-reduced free radical state. but these free radicals cannot be visualized by esr spectroscopy because of closeness and strong interaction. after the acid denaturation of the protein the coenzyme is released as a semi-reduced free radical. an altern ... | 1976 | 7743 |
| the fluorescence of pepsin conjugates with dns-chloride. | parameters of rotational relaxation of pepsin conjugated in neutral and slightly alkaline solutions with a fluorescent label 1-dimethylaminonaphthalene-5-sulphonyl chloride (dns-cl) are measured by a fluorescence polarization method. it is shown that the globule of pepsin denatured and loose at lakaline ph values converts into a compact form after transfer to acidic solution. the compactness of this new form is close to that of native inhibited pepsin. a new globule is distinguished from the nat ... | 1976 | 7744 |
| mechanism of the stimulating effect of pachycarpine on the uterus. | 1976 | 7764 | |
| gamma-glutamyl transpeptidase, a lymphoid cell-surface marker: relationship to blastogenesis, differentiation, and neoplasia. | gamma-glutamyl transpeptidase, an enzyme that catalyzes gamma-glutamyl transfer from gamma-glutamyl compounds to amino acid and peptide acceptors, and which is known to be localized in the membranes of many epithelial cells, was found in a variety of lymphoid cells. the lymphoid cell enzyme is located on the cell surface, and exhibits substantially the same substrate specificity as the enzyme found in epithelial cells. human and rat (but not mouse) lymphocyte gamma-glutamyl transpeptidase was st ... | 1976 | 7785 |
| the development of digestive function in the pig. | 1976 | 7786 | |
| effect of beta-adrenoceptor stimulators, papaverine and theophylline on guinea-pig trachea contracted by different drugs. | 1976 | 7916 | |
| mediation of macrophage effector function by lymphokine. | the progressive effect of lymphokine contact on macrophages in vitro has been studied using various quantitative cytochemical techniques. changes in the physiology of the macrophages have been seen rapidly after lymphokine contact. these appear to correlate with the functional effect of inhibition of migration. after more prolonged contact with lymphokine however, the macrophages exhibit different changes to their physiology and reach a state of enhanced cytochemical activity which has been term ... | 1976 | 7933 |
| a kinetic study of pig liver glucose dehydrogenase. | 1976 | 8012 | |
| inhibition by suprofen and other non-narcotic analgesic drugs of the effects of prostaglandin precursor on isolated tissues and platelets. | contractions caused by slow reacting substance c(srs-c) and by arachidonic acid hydroperoxide (aap) in the guinea-pig ileum and by aap in the rat fundus were studied in the presence of suprofen and of 3 reference compounds. the dose-related inhibitions were not due to antagonism of prostaglandins. other agonists of gastrointestinal smooth muscle were not or only weakly antagonized. for the study of selective inhibition of aap-induced contractions by non-narcotic analgesics, the rat fundus is the ... | 1976 | 8022 |
| inhibition of superoxide anion production in macrophages by anti-inflammatory drugs. | 1976 | 8043 | |
| basic ethers of cyclohexylphenols with beta-blocking activity: synthesis and pharmacological study of exaprolol. | the paper describes the preparation and study of the pharmacological properties of a series of derivatives with the general formula. (see article) where r is a cyclohexyl or cyclohexenyl radical and r1 is a hydrogen, methyl or cyclohexyl. the compounds with a single cycloaliphatic radical ortho to the basic chain, and in particular the one with a cyclohexyl (exaprolol), were found to be particularly active in blocking the beta-adrenergic receptors, as antiarrhythmics and local anesthetics, while ... | 1976 | 8056 |
| general pharmacological properties of a new non-opiate antitussive: zipeprol (3024 cerm). i. action on respiratory function and acute toxicity. | 1-(2-methoxy-2-phenyl)-ethyl-4-(2-hydroxy-3-methoxy-3-phenyl)-propyl-iperazine-dihydrochloride (zipeprol, respilene) is a substance of non-phenanthrenic chemical structure. in the cat, it antagonised cough induced by stimulation of the superior laryngeal nerve or by direct mechanical excitation of the sensitive tracheo-bronchial receptors. the efficacy of zipeprol after enteral administration made it possible both to establish good intestinal absorption and to rank it favourably in relation to s ... | 1976 | 8057 |
| characterization of 20s component in tubulin from mammalian brain. | tubulin from porcine brain, purified by at least two cycles of assembly and disassembly, was characerized at different ph values by sedimentation velocity analysis and turbidimetric measurements. at ph 6.4 the depolymerized material was composed of two major species sedimenting with so20,w values of 6 and 36 and a minor one of 20s. by raising the ph, the amount of the 20s component increased and that of the 36s decreased, whereas that of the 6s component was unaltered. at ph 7.6 the mixture cont ... | 1976 | 8078 |
| vinylglycine and proparglyglycine: complementary suicide substrates for l-amino acid oxidase and d-amino acid oxidase. | proparglyglycine (2-amino-4-pentynoate) and vinylglycine (2-amino-3-butenoate) have been examined as substrates and possible inactivators of two flavo enzymes, d-amino acid oxidase from pig kidney and l-amino acid oxidase from crotalus adamanteus venom. vinylglycine is rapidly oxidized by both enzymes but only l-amino acid oxidase is inactivated under assay conditions. the loss of activity probably involves covalent modification of an active site residue rather than the flavin adenine dinucleoti ... | 1976 | 8082 |
| isolation and characterization of a hemagglutinin from amphitrite ornata, a polychaetous annelid. | extracts of the marine polychaetous annelid, amphitrite ornata, agglutinate rat, rabbit, chicken and human erythrocytes and in other work have been shown to inhibit the growth of ehrlich ascites tumors in mice. fractionation of extracts on sephadex g-100 gave three active fractions with molecular weights of 30 000, 54 000 and 100 000. the 30 000 dalton fraction (b) was purified 72-fold by ammonium sulfate precipitation, gel filtration and preparative disc gel electrophoresis. the purified hemagg ... | 1976 | 8117 |
| chemical investigations on pig kidney aminoacylase. | 1. preparations of purified pig kidney aminoacylase (n-acylamino-acid amidohydrolase, ec 3.5.1.14) were obtained by sephadex and deae-cellulose chromatography in homogeneous form as judged by polyacrylamide gel electrophoresis and immunoelectrophoresis. 2. the apparent molecular weight of the enzyme, determined by gel filtration, was about 86 000. after treatment with mercaptoethanol, performic acid or sodium dodecyl sulphate a band with an apparent molecular weight of approximately 43 000 was o ... | 1976 | 8149 |
| the reaction of the histidine residues of luteinizing hormone with ethoxyformyl anhydride. | bovine and porcine luteinizing hormones (b-lh, p-lh) and their subunits were treated by ethoxyformyl anhydride. the acylation of the histidine residues was followed by examination of the absorbance spectrum. all the histidine residues of the luteinizing hormone molecule can be modified at ph5. however 2 his in b-lh and 1 in p-lh appear to be much less reactive at ph 5 than the others and their acylated imidazols more labile at the same ph. at neutral ph, 2 histidines in b-lh (and 1 in p-lh) beco ... | 1976 | 8157 |
| resistance of alkylated dna to degradation by deoxyribonuclease ii at neutral and acid ph. | methylation of a calf thymus dna substrate by dimethyl sulphate (dms) leads to an inhibition of deoxyribonuclease ii activity which is gradually lost with time. the extent of this initial inhibition is linearly related to the amount of methylated products in dna and quantitatively similar effects were found when the enzyme was used under either acid or neutral conditions. deoxyribonuclease ii was shown to produce 3'-phosphate termini under both acid and neutral conditions and thus, irrespective ... | 1976 | 8218 |
| arylesterases from various mammalian sera in relation to cholinesterases, carboxylesterases and their activity towards some pesticides. | 1976 | 8268 | |
| inactivation of porcine calcitonin by rat kidney microsome. | biological activity of porcine calcitonin was most actively inactivated by the rat kidney homogenate than by other tissue homogenates. among the various subcellular fractions of the rat kidney homogenate examined, microsome fraction was most active in the in vitro inactivation of porcine calcitonin. inactivation of porcine calcitonin by the rat kidney microsome was dependent on ph and temperature. inactivating activity of the rat kidney microsome was inhibited by 1 x 10(-3) m monoiodoacetate and ... | 1976 | 8306 |
| chirality of xylitol-oxidizing enzymes from mammalian liver. | 1976 | 8339 | |
| partial purification of pig aorta amine oxidases. | two amine oxidases have been partially purified from pig aorta and their porperties investigated: one is similar to pig plasma oxidase (benzylamine oxidase), the other to lysyloxidase. both are inhibited by carbonyl reagents and cupric copper chelating agents, but they strongly differ in the kinetic properties. the benzylamine oxidase has a maximal reaction rate at acid ph values, the lysyloxidase has an optimum around ph 6.8. | 1976 | 8405 |
| inhibitory activity of pyridindolol on beta-galactosidase. | the activity of pyridindolol in inhibiting beta-galactosidases obtained from various sources has been studied. whereas acid bovine liver beta-galactosidase (optimal ph 4.0) was not affected by this compound, neutral bovine liver beta-galactosidase (ph-optimum = 7.0) was inhibited by pyridindolol in reaction mixtures of ph 4.0 approximately 5.0. there was no inhibition at ph 7.0. the type of inhibition is non-competitive by formation of a pyridindolol-enzyme complex. since beta-galactosidases fro ... | 1976 | 8415 |
| nucleophilic addition reactions of free and enzyme-bound deazaflavin. | deazafmn-containing glycolate oxidase has been prepared and shown to catalyze the stereospecific transfer of the alpha-hydrogen from substrate to enzyme-bound deazafmn. the reaction of sulfite, cyanide, and hydroxylamine with several deazaflavin-containing enzymes (glycolate oxidase, d-amino acid oxidase, glucose oxidase, n-methylglutamate synthetase) and free deazafmn has been examined. all the deazaflavin systems tested form reversible 1:1 complexes with sulfite and cyanide. the ph dependence ... | 1976 | 8450 |
| anti-inflammatory actions of dapsone and its related biochemistry. | dapsone has been examined by two established animal anti-inflammatory models and found to possess anti-inflammatory activity comparable with established non-steroidal anti-inflammatory drugs. dapsone also possess some biochemical properties common to other anti-inflammatory drugs. | 1976 | 8608 |
| proceedings: effects of sympathomimetic amines and ouabain on potassium influx in guinea-pig liver slices. | 1976 | 8635 | |
| proceedings: effects of palythoatoxin of palythoa tuberculosa on smooth muscles. | 1975 | 8656 | |
| evidences for alpha excitatory action of catecholamines on the electrical activity of the guinea-pig stomach. | effects of catecholamines and their inhibitors on the electrical activity of the smooth muscle of the guinea-pig stomach were studied by using pressure electrodes. the spontaneous electrical activity of antrum and corpus preparations consisted of slow waves with or without superimposed spikes. in quiescent fundus preparations, the spike activity and slow waves were generated by tea. catecholamines (noradrenaline, isoprenaline and adrenaline) suppressed or blocked the spike discharges and the gen ... | 1976 | 8660 |
| the haemolytic effect of phallolysin. | phallolysin from the toadstool, amanita phalloides, is a basic protein that causes direct haemolysis of red cells. the dose-response curve is steep; the ph optimum is in the weakly acid range. the rate of haemolysis increases with the concentration of the lysin, the optimal temperature is 20 degrees c. the percentage haemolysis-time curves are s-shaped. haemolysis is of the non-osmotic type. ca2+ is not required but inhibits haemolysis in a concentration-dependent fashion, as do mg2+ and zn2+. t ... | 1976 | 8736 |
| subunit structure and isozymic forms of gamma-glutamyl transpeptidase. | gamma-glutamyl transpeptidase is associated with the membranes of a number of epithelial and lymphoid cells. when the enzyme is isolated from rat kidney by a method involving detergent extraction and affinity chromatography, an aggregate of molecular weight greater than 200,000 (heavy form) is obtained. treatment of the heavy form with bromelain yields a light form of the enzyme (molecular weight of approximately 68,000), which is separable by isoelectric focusing into 12 enzymatically active is ... | 1976 | 8776 |
| heteroallergic leukocytic migration inhibition in pulmonary tuberculosis. | 1976 | 8905 | |
| arterial elastin. | 1976 | 8978 | |
| bêta-adrenoreceptor blocking properties of 1-nitro, hydroxy phenyl, n-benzoyl alanine (gal-1). | 1976 | 9003 | |
| sympathomimetic-induced effects in the soleus muscle of the guinea-pig. | sympathomimetic amines reduced the peak tension, time to peak and time to half-relaxation of indirectly elicited twitches of the guinea-pig soleus muscle in vivo. clonic contractions of the soleus muscle were depressed by the amines. on a molar basis salbutamol and orciprenaline were 5.12 and 80.0 times less potent than (+/-)-isoprenaline in producing these effects. results obtained with the beta-receptor antagonists propranolol, practolol and h35/25 suggest that the depression in skeletal muscl ... | 1976 | 9032 |
| electrophysiologically determined quinidine-like actions of a beta-adrenergic blocking agent, 1-(7-indenyloxy)-3-isopropylaminopropane-2-ol hydrochloride (yb-2). | since 1-(7-indenyloxy)-3-isopropylaminopropane-2-ol hydrochloride (yb-2) has been reported to be a potent beta-adrenergic blocking agent with antiarrhythmic effects, the electrophysiological effects of this compound on guinea-pig and canine heart muscle were examined. yb-2 was found to have typical quinidine-like actions such as decrease in rate of rise of the action potential, prolongation of refractory period and decrease in conduction velocity. | 1976 | 9035 |
| specific gamma-glutamyl transpeptidase inhibitor from human and animal intestines. | an inhibitor of gamma-glutamyl transpeptidase in human and animal intestines was assayed by means of a new method. in mice fed with lsm fodder, accumulation of the inhibitor in the mucous membrane of the small intestine was observed. purified gamma-glutamyl transpeptidase inhibitor from mouse and human intestines was identified as l-serine. neither this amino acid nor purifed inhibitor acted on other intestinal peptidases. presumably, one of the ingredients of lsm feed influences accumulation of ... | 1976 | 9045 |
| species differences in the conjugation of 4-hydroxy-3-methoxyphenylethanol with glucuronic acid and sulphuric acid. | the biosynthesis of the glucuronide and sulphate conjugates of 4-hydroxy-3-methoxyphenylethanol was demonstrated in vitro by using the high-speed supernatant and microsomal fractions of liver respectively. these two conjugates were also produced simultaneously by using the post-mitochondrial fraction of rat, rabbit or guinea-pig liver. in contrast only the glucuronide was synthesized by human liver and only the sulphate by mouse and cat livers. neither of these conjugates was formed by the kidne ... | 1976 | 9078 |
| some aspects in the pharmacology of diclonium bromide (2-(3,2-dichloroanilino)quinolizinium bromide). part i: antispasmodic action. | diclonium bromide (eu-2972; 2-(3,4-dichloroanilino)quinolizinium bromide) was demonstrated to possess an effective, prolonged inhibitory action on contractions in response to stimuli or propulsive movements in the lower bowel of the dog. the compound's antagonism to contractile activity was greater in the distal colon than in the duodenum or upper bowel. diclonium bromide was a nonselective antispasmodic but, unlike papaverine, caused profound antagonism against smooth muscle contractile respons ... | 1976 | 9098 |
| proceedings: the primary process of vascular smooth muscle contraction and the influence of stress, temperature, ca++, h+, and norepinephrine. | 1976 | 9115 | |
| porcine malignant hyperthermia. iii: adrenergic blockade. | the effects of the establishment of an adrenergic blockade on suxamethonium-induced porcine malignant hyperthermia (mh) were investigated in pietrain pigs. six animals were fed reserpine 10 mg daily for 7 days and then challenged with suxamethonium. three survived but the remainder developed fatal mh. in a further study of 10 pigs, either phentolamine 40 mug/kg/min or propranolol 50 mug/kg/min were administered for 30 min before suxamethonium stimulation and continued for the duration of the exp ... | 1976 | 9123 |
| influence of substrates and coenzymes on the role of manganous ion in reactions catalyzed by pig heart triphosphopyridine nucleotide-dependent isocitrate dehydrogenase. | the interaction of manganous ions with pig heart triphosphopyridine nucleotide (tpn) specific isocitrate dehydrogenase has been studied by kinetic experiments and by direct ultrafiltration measurements of manganous ion binding. at low metal ion concentrations, a lag is observed in the time-dependent production of reduced triphosphopyridine nucleotide (tpnh) that can be eliminated by adding 20 mum tpnh to the initial reaction mixture. a plot of 1/upsilon vs. 1/ (mn2+) obtained at relatively high ... | 1976 | 9128 |
| thyroid ribonucleic acid-iodopeptides. comparison of tyrosyl-complex ii and tyrosyl-trna. | it has previously been shown that mammalian rna-peptidyl complexes are found in close association with trna, but can be separated from the bulk of the trna by benzoylated diethylaminoethylcellulose chromatography (kull, f.j., and soodak, m. (1971), biochim. biophys. acta 246, l; gadski, r.a., and kull, f.j. (1973), biochemistry 12, 1907). these studies also showed that under aminoacylation conditions the complex fractions were able to act as acceptors for certain amino acids and that the formati ... | 1976 | 9130 |
| the interaction of radioiodinated thyrotropin with plasma membranes. evidence for high affinity binding sites in the thyroid. | the binding of biologically active 125ithyrotropin to purified plasma membranes prepared from bovine thyroid glands was studied. at 4 degrees c, specific binding reached a maximum after 2 h of incubation and a plateau was maintained for up to 20 h. degradation of 125ithyrotropin was undetectable after 2 h of incubation and was only 10% of the total after 20 h. at ph 6.0, at which binding was maximal, a single class of binding sites, having a dissociation constant of approx. 25 nm, was evident. d ... | 1976 | 9155 |
| higher transpeptidation activity and broad acceptor specificity of gamma-glutamyltransferases of tumors. | the gamma-glutamyltransferase (ec 2.3.2.2) (=gamma-glutamyltranspeptidase, gamma-gtp) activity in hepatoma induced by 3'-methyl-4-(dimethylamino)azobenzene (3'-me-dab) was 120-fold higher than that of normal liver and high activity was also found in bovine hepatocellular carcinoma. gamma-gtps from these malignant tissues responded more and showed broader specificity to gamma-glutamyl group acceptors than those from normal tissue such as bovine, rat, and mouse liver and bovine kidney. three speci ... | 1976 | 9327 |
| a kallikrein-specific inhibitor in rat kidney tubules. | a kallikrein inhibitor was found in tubules of the rat kidney and purified by chromatography on sephadex g-100. the molecular weight of the inhibitor, estimated by gel filtration and dodecylsulfate electrophoresis, is about 4700. it inhibits the following kallikreins: porcine submanidbular and pancreatic kallikrein, rat kidney and urine kallikrein, and human urine and plasma kallikrein. an inhibition of bovine trypsin was not observed. | 1976 | 9350 |
| facile alkylation of methionine by benzyl bromide and demonstration of fumarase inactivation accompanied by alkylation of a methionine residue. | benzyl bromide is a selective alkylator of sulfur nucleophiles including methionine and cysteine. only the mercaptide ion is a more efficient nucleophile than is the sulfur ether of methionine. alkylation rates relative to methionine are 200: less than or equal to 0.03: less than or equal to 0.03: less than or equal to 0.02 for gs-, histidine, tryptophan, and gsh, respectively. alkylation of methionine by benzyl bromide is more than 50 times faster than alkylation by iodoacetate. fumarase is rea ... | 1976 | 9397 |
| active site chlorination of d-amino acid oxidase by n-chloro-d-leucine. | n-chloro-d-leucine is an irreversible inhibitor or d-amino acid oxidase on a time scale of seconds. studies with n-36cchloro-d-leucine, n-chloro-d-1-14cleucine and n-chloro-d-4,5-3hleucine show that the modified enzyme has been chlorinated at a site, or sites, on the apoenzyme. the 36cl measurements agree with titrations of catalytic activity in showing that two chlorine equivalents are incorporated per active site flavin. kinetically, the interaction with n-chloro-d-leucine behaves in a manner ... | 1976 | 9413 |
| utilization of volatile fatty acids in ruminants. 8. acetate activation in mammary tissue. | 1976 | 9435 | |
| the role of lipids in the observed lack of phosphatidylcholine exchange in myelin. | 1976 | 9490 | |
| the detection of toxin production by corynebacterium diphtheriae in diagnostic laboratories. | in vitro tests for assessing the toxigenicity of strains of c. diphtheriae using the original agar plate precipitin method of elek are unreliable. at high concentrations of antitoxin (500-1000 u/ml) multiple non-specific precipitin lines occur making interpretation difficult. increased specificity was obtained by diluting the antitoxin but this in turn caused a delay in the appearance of toxin-antitoxin lines. also, the failure of lines to appear on media enriched with unsuitable serum may lead ... | 1976 | 9614 |
| novikoff hepatoma deoxyribonucleic acid polymerase. sensitivity of the beta-polymerase to sulfhydryl blocking agents. | unlike other beta-class eukaryotic dna polymerases, the enzyme purified from the novikoff hepatoma is inhibited by both sulfhydryl blocking agents n-ethylmaleimide (nem) and p-hydroxymercuribenzoate (phmb). the degree of sensitivity varies depending on the enzyme purity, ph of the reaction, and the presence of sulfhydryl reducing agents. novikoff beta-polymerase activity is unaffected by the presence of 2-mercaptoethanol (2-me) or dithiothreitol (dtt); however, the combination of 2-mercaptoethan ... | 1976 | 9624 |
| some effects of processing on the nutritive value of feedstuffs for growing pigs. | 1976 | 9645 | |
| in vitro studies on the antimicrobial effects of colostrum and milk from vaccinated and unvaccinated pigs on escherichia coli. | unsupplemented porcine colostrum and milk exhibited a powerful bactericidal effect for porcine strains of e coli incubated in vitro at 37 degrees c. this activity was independent of complement but was susceptible to acid ph, to the presence of soluble iron and to the selective immunoprecipitation of igg, iga and igm. manifestation of bactericidal activity required bacteria in an active state of metabolism and the length of incubation was an important factor in demonstrating the quality of the an ... | 1976 | 9677 |
| latent pseudorabies virus infection in pigs. | latent infection with the top strain of pseudorabies virus (prv) was established in 6 weeks old piglets. infectious virus was found in oropharyngeal swabs till day 10 post infection (p.i.); later on, attempts to detect the virus remained unsuccessful. however, prv could be isolated by explantation of tonsils, cervical lymph nodes, nasal mucosa and gasserian ganglia. these tissues were removed between 160 and 181 days p.i., during cultivation, infectious prv was released into the culture fluid fr ... | 1976 | 9801 |
| in vitro activation of glycoprotein hormones. hybridization of subunits from thyrotropin, lutropin and human choriogonadotropin. | in vitro assembly of thyrotropin alpha and beta subunits led to an increase in content of alpha helix and beta sheet very similar to that found for gonadotropins. this association-dependent active folding involved the burying of three tyrosine residues tentatively assigned to tyr alpha 41, tyr beta 37 and tyr beta 59 and common to all studied glycoprotein hormones. in vitro hybridizations between alpha and beta subunits of various hormones (thyrotropin, lutropin and choriogonadotropin) from diff ... | 1976 | 9999 |
| the contribution of extraneuronal uptake to the trachea-blood vessel selectivity of beta-adrenoceptor stimulants in vitro in guinea-pigs. | 1 the potencies relative to isoprenaline of isoetharine, tertiary butyl noradrenaline, salbutamol, orciprenaline, me 506, rimiterol, fenoterol, carbuterol and terbutaline on isolated preparations of guinea-pig trachea and blood vessels (perfused hind limb) were determined. all the compounds were selective for trachea and selectivity values, i.e. relative potency on trachea divided by relative potency on hind limb, ranged from 2.3 to 21.4. 2 responses to isoprenaline (the reference compound), ter ... | 1976 | 10029 |
| profiles of different anti-allergy effects of a new histamine antagonist, bm 15,100 proceedings. | 1976 | 10032 | |
| anti-allergic properties of a new coumarin compound (bm 15,100) proceedings. | 1976 | 10033 | |
| adrenoreceptors of the guinea-pig urinary bladder. | 1 adrenaline, noradrenaline and isoprenaline (5 mug/ml) did not affect the resting tone of the isolated urinary bladder of the guinea-pig. 2 the catecholamines (1-2 mug/ml) inhibited neuronally evoked contractions at various stimulation frequencies; the inhibition was maximum at 2 hz and minimum at 50 hz. isoprenaline produced maximum inhibition. 3 propranolol (0.5 mug/ml) completely blocked the catecholamine-induced inhibition at all the frequencies employed. the concentration-response curves o ... | 1976 | 10034 |
| further characterization of morphine-like peptides (endorphins) from pituitary. | pituitary peptides with opioid pharmacologic action (endorphins) can exist in several forms with different basicities and molecular weights from about 3000 to less than 1000 daltons. partial purification yielded an endorphin at least 4 times more potent than normorphine and 16 times more potent than the endogenous brain pentapeptide methionine enkephalin. it is proposed that pituitary endorphin or a similar peptide in brain may be the precursor of brain enkephalin. | 1976 | 10049 |
| comparative study of pentose phosphate pathway enzymes in acanthocephalans from homothermic and poikilothermic hosts. | 1976 | 10129 | |
| studies on the kinetic effects of adenosine-3':5'-monophosphate-dependent phosphorylation of purified pig-liver pyruvate kinase type l. | the effect of cyclic-amp-dependent phosphorylation on the activity of isolated pig liver pyruvate kinase was studied. it was found that the major kinetic effect of the phosphorylation was to reduce the affinity for the substrate phosphoenolpyruvate, k0.5 for this substrate increasing from 0.3 to 0.9 mm upon phosphorylation. the cooperative effect with phosphoenolpyruvate was enhanced, the hill constant nh increasing concomitantly from 1.1 to 1.5. v was unaltered. the change in activity occurred ... | 1976 | 10159 |
| lipoperoxidation rates and drug-oxidizing enzyme activities in the liver and placenta of some mammal species during the perinatal period. | lipoperoxidation and drug-metabolizing enzymes were measured in livers and placentas of different mammal species during the perinatal perios. in placentas and fetal livers of rat, rabbit and guinea-pig, cofactor-supported lipoperoxidation was negligible, as were the activities of drug-oxidizing enzymes. human fetal liver contained an intact drug-oxidizing electron transport chain, and lipoperoxidation activity was accordingly abserved. it is suggested that lesions mediated by lipoperoxidation ma ... | 1976 | 10178 |
| a pharmacological study of the angiotensin receptor and tachyphylaxis in smooth muscle. | the interaction of angiotensin with its receptor has been studied on the basis of the tachyphylaxis shown by the rat uterus towards angiotensin ii when ph and ca2+ concentration are below physiological levels. 14c-angiotensin binding and 45ca2+-uptake investigations suggest tachyphylaxis to be due to increased binding at low ph and ca2+ concentration. studies with alkylating (affinity labeled) angiotensin derivatives containing the n-mustard chlorambucil suggest a "charnière type" inhibition at ... | 1976 | 10223 |
| renal angiotensin i-converting enzyme as a mixture of sialo- and asialo-enzyme, and a rapid purification method. | angiotensin i-converting enzyme ec 3.4.15.1 was rapidly and highly purified from a particulate fraction of hog kidney cortex with 13% yield. the procedure, which was rapid, included fractionation on deae-cellulose and calcium phosphate gel, chromatographies on deae-sephadex a-50 and hydroxylapatite columns, and gel filtration on a sephadex g-200 column. the purified enzyme preparation gave two protein bands on standard disc gel electrophoresis, but showed a single protein component on the gel af ... | 1976 | 10287 |