Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| antivenom cross neutralisation in a suspected asian pit viper envenoming causing severe coagulopathy. | there is evidence of cross-neutralisation between common toxin groups in snake venoms and therefore the potential for antivenoms to be effective against species they are not raised against. here we present a 49 year old female bitten by an unknown pit-viper in nepal. she developed a venom induced consumption coagulopathy with an unrecordable international normalised ratio and undetectable fibrinogen. on return to australia 5 days post-bite she was treated successfully with one antivenom raised a ... | 2014 | 25178876 |
| proteomic investigation of sri lankan hump-nosed pit viper (hypnale hypnale) venom. | the hump-nosed pit viper, hypanle hypnale, contributes to snakebite mortality and morbidity in sri lanka. studies showed that the venom is hemotoxic and nephrotoxic, with some biochemical and antigenic properties similar to the venom of calloselasma rhodostoma (malayan pit viper). to further characterize the complexity composition of the venom, we investigated the proteome of a pooled venom sample from >10 sri lankan h. hypnale with reverse-phase high performance liquid chromatography (rp-hplc), ... | 2015 | 25451538 |
| heterochrony and early left-right asymmetry in the development of the cardiorespiratory system of snakes. | snake lungs show a remarkable diversity of organ asymmetries. the right lung is always fully developed, while the left lung is either absent, vestigial, or well-developed (but smaller than the right). a 'tracheal lung' is present in some taxa. these asymmetries are reflected in the pulmonary arteries. lung asymmetry is known to appear at early stages of development in thamnophis radix and natrix natrix. unfortunately, there is no developmental data on snakes with a well-developed or absent left ... | 2015 | 25555231 |
| effective equine immunization protocol for production of potent poly-specific antisera against calloselasma rhodostoma, cryptelytrops albolabris and daboia siamensis. | snake envenomation has been estimated to affect 1.8 million people annually with about 94,000 deaths mostly in poor tropical countries. specific antivenoms are the only rational and effective therapy for these cases. efforts are being made to produce effective, affordable and sufficient antivenoms for these victims. the immunization process, which has rarely been described in detail, is one step that needs to be rigorously studied and improved especially with regard to the production of polyspec ... | 2015 | 25774998 |
| neurological involvement and hepatocellular injury caused by a snake with hematotoxin envenomation. | venomous snakes with hematotoxin-russell's viper (daboia spp), malayan pit viper (calloselasma rhodostoma), and green pit viper (cryptelytrops albolabris and c macrops, previously named trimeresurus spp) are commonly found in thailand. coagulation factor activation, thrombocytopenia, hyperfibrinolysis, and disseminated intravascular coagulation are the main mechanisms of hemorrhaging from these snake bites. the neurological involvement and hepatocellular injury after russell's viper bites were r ... | 2015 | 25890858 |
| evaluating the microbicidal, antiparasitic and antitumor effects of cr-laao from calloselasma rhodostoma venom. | cr-laao is an l-amino acid oxidase from calloselasma rhodostoma snake venom that has been broadly studied regarding its structural and biochemical characteristics, however, few studies have investigated its pharmacological effects. the present study aimed at the evaluation of the biotechnological potential of cr-laao by determining its bactericidal, antifungal, leishmanicidal and trypanocidal activity, as well as its cytotoxicity on human tumor and non-tumor cell lines. after 24 h of preincubati ... | 2015 | 26162245 |
| molecular barcoding of venomous snakes and species-specific multiplex pcr assay to identify snake groups for which antivenom is available in thailand. | dna barcodes of mitochondrial coi and cytb genes were constructed from 54 specimens of 16 species for species identification. intra- and interspecific sequence divergence of the coi gene (10 times) was greater than that of the cytb gene (4 times), which suggests that the former gene may be a better marker than the latter for species delimitation in snakes. the coi barcode cut-off scores differed by more than 3% between most species, and the minimum interspecific divergence was greater than the m ... | 2015 | 26535713 |
| procoagulant snake venoms have differential effects in animal plasmas: implications for antivenom testing in animal models. | animal models are used to test toxic effects of snake venoms/toxins and the antivenom required to neutralise them. however, venoms that cause clinically relevant coagulopathy in humans may have differential effects in animals. we aimed to investigate the effect of different procoagulant snake venoms on various animal plasmas. | 2016 | 26656242 |
| iron modulates the alpha chain of fibrinogen. | iron-bound fibrinogen has been noted to accelerate plasmatic coagulation in patients with divergent conditions involving upregulation of heme oxygenase activity, including hemodialysis, alzheimer's disease, sickle cell anemia, and chronic migraine. our goal was to determine if a site of iron-fibrinogen interaction was on the alpha chain. using thrombelastography, we compared the coagulation kinetic profiles of plasma exposed to 0-10 µm ferric chloride after activation of coagulation with thrombi ... | 2016 | 26782808 |
| cr-laao antileukemic effect against bcr-abl(+) cells is mediated by apoptosis and hydrogen peroxide. | chronic myeloid leukemia (cml) is a myeloproliferative neoplasm characterized by the presence of the bcr-abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. tyrosine kinase inhibitors (tkis) are effectively used to treat cml; however, cml patients in the advanced (cml-ap) and chronic (cml-cp) phases of the disease are usually resistant to tki therapy. thus, it is necessary to seek for novel agents to treat cml, such as the enzyme l-amino acid oxidase from callos ... | 2016 | 26812110 |
| ancrod revisited: viscoelastic analyses of the effects of calloselasma rhodostoma venom on plasma coagulation and fibrinolysis. | fibrinogen depletion via catalysis by snake venom enzymes as a therapeutic strategy to prevent or treat thrombotic disorders was utilized for over four decades, with ancrod being the quintessential agent. however, ancrod eventually was found to not be of clinical utility in large scale stroke trial, resulting in the eventual discontinuation of the administration of the drug for any indication. it was hypothesized that ancrod, possessing thrombin-like activity, may have unappreciated robust coagu ... | 2016 | 26905070 |
| prospective, consecutive case series of 158 snakebite patients treated at savannakhet provincial hospital, lao people's democratic republic with high incidence of anaphylactic shock to horse derived f(ab')2 antivenom. | snakebites are a seriously neglected public health problem in lao pdr. community-based cross-sectional surveys in two districts of savannakhet province in southern laos revealed an incidence of up to 1105 snakebites per 100,000 persons per year. in contrast the number of snakebite patients treated in district and provincial hospitals are low. in order to improve health care for snakebite victims, antivenom was introduced to savannakhet provincial hospital in july 2013 and medical staff has been ... | 2016 | 26995210 |
| l-amino acid oxidase isolated from calloselasma rhodostoma snake venom induces cytotoxicity and apoptosis in jak2v617f-positive cell lines. | myeloproliferative neoplasms are philadelphia chromosome-negative diseases characterized by hyperproliferation of mature myeloid cells, associated or not with the janus kinase 2 tyrosine kinase mutation, jak2v617f. as there is no curative therapy, researchers have been investigating new drugs to treat myeloproliferative neoplasms, including l-amino acid oxidase from calloselasma rhodostoma snake venom (cr-laao), which is a toxin capable of eliciting apoptosis in several tumor cell lines. | 2017 | 27208571 |
| venomics of calloselasma rhodostoma, the malayan pit viper: a complex toxin arsenal unraveled. | the venom of malayan pit viper (calloselasma rhodostoma) is highly toxic but also valuable in drug discovery. however, a comprehensive proteome of the venom that details its toxin composition and abundance is lacking. this study aimed to unravel the venom complexity through a multi-step venomic approach. at least 96 distinct proteins (29 basic, 67 acidic) in 11 families were identified from the venom. the venom consists of mainly snake venom metalloproteinases (svmp, 41.17% of total venom protei ... | 2016 | 27418434 |
| the l-amino acid oxidase from calloselasma rhodostoma snake venom modulates apoptomirs expression in bcr-abl-positive cell lines. | anti-apoptotic genes and apoptomirs deregulated expression contribute to apoptosis resistance in chronic myeloid leukemia (cml) bcr-abl(+) cells. here, the l-amino acid oxidase from calloselasma rhodostoma (cr-laao) venom altered the apoptotic machinery regulation by modulating the expression of the mir-145, mir-26a, mir-142-3p, mir-21, mir-130a, and mir-146a, and of the apoptosis-related proteins bid, bim, bcl-2, ciap-2, c-flip, and mcl-1 in bcr-abl(+) cells. cr-laao is a potential tool to inst ... | 2016 | 27421670 |
| carbon monoxide releasing molecule-2 inhibition of snake venom thrombin-like activity: novel biochemical "brake"? | a complication of defibrinogenation therapy with snake venom enzymes such as ancrod is hypofibrinogenemia associated bleeding secondary to no human-derived inhibitor being available to inactivate or diminish the activity of such enzymes. of interest, ancrod contains a critical histidine residue without which enzymatic activity is inhibited, and carbon monoxide has been demonstrated to inhibit biomolecular function by interacting with histidine moieties in ion channels. we tested the hypothesis t ... | 2017 | 27787696 |
| assessing sabu (serum anti bisa ular), the sole indonesian antivenom: a proteomic analysis and neutralization efficacy study. | serum anti ular bisa (sabu) is the only snake antivenom produced locally in indonesia; however, its effectiveness has not been rigorously evaluated. this study aimed to assess the protein composition and neutralization efficacy of sabu. sds polyacrylamide gel electrophoresis, size-exclusion liquid chromatography and shotgun proteomics revealed that sabu consists of f(ab')2but a significant amount of dimers, protein aggregates and contaminant albumins. sabu moderately neutralized calloselasma rho ... | 2016 | 27869134 |
| snake genome sequencing: results and future prospects. | snake genome sequencing is in its infancy-very much behind the progress made in sequencing the genomes of humans, model organisms and pathogens relevant to biomedical research, and agricultural species. we provide here an overview of some of the snake genome projects in progress, and discuss the biological findings, with special emphasis on toxinology, from the small number of draft snake genomes already published. we discuss the future of snake genomics, pointing out that new sequencing technol ... | 2016 | 27916957 |
| cr-laao, an l-amino acid oxidase from calloselasma rhodostoma venom, as a potential tool for developing novel immunotherapeutic strategies against cancer. | l-amino acid oxidases from snake venoms have been described to possess various biological functions. in this study, we investigated the inflammatory responses induced in vivo and in vitro by cr-laao, an l-amino acid oxidase isolated from calloselasma rhodostoma venom, and its antitumor potential. cr-laao induced acute inflammatory responses in vivo, with recruitment of neutrophils and release of il-6, il-1β, ltb4 and pge2. in vitro, il-6 and il-1β production by peritoneal macrophages stimulated ... | 2017 | 28205610 |
| dramatic and concerted conformational changes enable rhodocetin to block α2β1 integrin selectively. | the collagen binding integrin α2β1 plays a crucial role in hemostasis, fibrosis, and cancer progression amongst others. it is specifically inhibited by rhodocetin (rc), a c-type lectin-related protein (clrp) found in malayan pit viper (calloselasma rhodostoma) venom. the structure of rc alone reveals a heterotetramer arranged as an αβ and γδ subunit in a cruciform shape. rc specifically binds to the collagen binding a-domain of the integrin α2 subunit, thereby blocking collagen-induced platelet ... | 2017 | 28704364 |
| cross reactivity and lethality neutralization of venoms of indonesian trimeresurus complex species by thai green pit viper antivenom. | arboreal pit vipers of the trimeresurus complex group are medically important species in indonesia (west of wallace's line), but there is no specific antivenom produced in the country for treating related envenomation. instead, the exiting trivalent indonesian antivenom, biosave®serum anti bisa ular (sabu, indicated for envenoming by malayan pit viper, javan spitting cobra and banded krait) is often misused to treat trimeresus envenoming resulting in poor therapeutic outcome. here, we investigat ... | 2017 | 29051104 |
| functional characterization of recombinant snake venom rhodocytin: rhodocytin mutant blocks clec-2/podoplanin-dependent platelet aggregation and lung metastasis. | essentials we generated recombinant rhodocytin that could aggregate platelets via clec-2. recombinant wild-type rhodocytin formed heterooctamer with four α- and β-subunits. asp 4 in α-subunit of rhodocytin was required for binding to clec-2. inhibitory mutant of rhodocytin blocked podoplanin-dependent hematogenous metastasis. | 2018 | 29488681 |
| role of l-amino acid oxidase isolated from calloselasma rhodostoma venom on neutrophil nadph oxidase complex activation. | the action of cr-laao, an l-amino acid oxidase isolated from calloselasma rhodosthoma snake venom, on nadph oxidase activation in isolated human neutrophil function was investigated. this enzyme has an intrinsic activity of hydrogen peroxide production. cr-laao, in its native form, induces the ros production in neutrophil and migration of cytosolic nadph oxidase components p40phox, p47phox and p67phox to the membrane, and rac, a gtpase protein member, with the involvement of intracellular signal ... | 2018 | 29499246 |
| cr-laao causes genotoxic damage in hepg2 tumor cells by oxidative stress. | snake venom l-amino acid oxidases (sv-laaos) are enzymes of great interest in research due to their many biological effects with therapeutic potential. cr-laao, an l-amino acid oxidase from calloselasma rhodostoma snake venom, is a well described sv-laao with immunomodulatory, antiparasitic, microbicidal, and antitumor effects. in this study, we evaluated the genotoxic potential of this enzyme in human peripheral blood mononuclear cells (pbmc) and hepg2 tumor cells, as well as its interaction wi ... | 2018 | 29738841 |
| cytotoxic, antiproliferative and apoptosis-inducing activity of l-amino acid oxidase from malaysian calloselasma rhodostoma on human colon cancer cells. | the aim of this study was to investigate the cytotoxic, antiproliferative activity and the induction of apoptosis by l-amino acid oxidase isolated from calloselasma rhodostoma crude venom (cr-laao) on human colon cancer cells. cr-laao was purified using three chromatographic steps: molecular exclusion using g-50 gel filtration resin, ion-exchange by monoq column and desalted on a g25 column. the purity and identity of the isolated cr-laao was confirmed by sds-page and lc-ms/ms. cr-laao demonstra ... | 2018 | 29908095 |
| potential applications of venom peptides as anti-thrombotic agents for management of arterial and deep-vein thrombosis. | thrombus is composed of two main substances i.e. red blood cells and aggregated platelets which make a web of inter-connected fibrin proteins. during injury it prevents bleeding, so it is very useful but it can be very dangerous if it is produced in healthy blood vessels and block the blood flow through it. mural thrombi attaches with the blood vessels but in most cases do not block it completely. venoms are an incredible source of peptides having amazing bioactivities with varying number of ami ... | 2018 | 29921198 |
| proteomic characterization of two medically important malaysian snake venoms, calloselasma rhodostoma (malayan pit viper) and ophiophagus hannah (king cobra). | calloselasma rhodostoma (cr) and ophiophagus hannah (oh) are two medically important snakes found in malaysia. while some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and distribution of snake venom toxins, resulting in variations in venom composition. therefore, a combined proteomic approach using shotgun and gel filtration chromatography, analyzed by tandem mass spectrometry, was used to examine the comp ... | 2018 | 30373186 |
| basal but divergent: clinical implications of differential coagulotoxicity in a clade of asian vipers. | envenomations by asian pitvipers can induce multiple clinical complications resulting from coagulopathic and neuropathic effects. while intense research has been undertaken for some species, functional coagulopathic effects have been neglected. as these species' venoms affect the blood coagulation cascade we investigated their effects upon the human clotting cascade using venoms of species from the azemiops, calloselasma, deinagkistrodon and hypnale genera. calloselasma rhodostoma, deinagkistrod ... | 2019 | 30930232 |
| comparative proteomes, immunoreactivities and neutralization of procoagulant activities of calloselasma rhodostoma (malayan pit viper) venoms from four regions in southeast asia. | the intraspecific geographical venom variations of calloselasma rhodostoma from malaysia (cr-m), indonesia (cr-i), thailand (cr-t) and vietnam (cr-v) were investigated through 1d sds-page and nano-esi-lcms/ms. the venom antigenicity, procoagulant activities and neutralization using thai c. rhodostoma monovalent antivenom (crmav) were also investigated. sds-page patterns of the venoms were relatively similar with minor variations. proteomic analysis revealed that snake venom metalloproteinases (s ... | 2019 | 31445943 |
| snake venom rhodocytin induces plasma extravasation via toxin-mediated interactions between platelets and mast cells. | venomous snakebites can induce local tissue damage, including necrosis of soft tissues, haemorrhage, blistering and local swelling associated with plasma extravasation, which can lead to lethal complications such as hypovolemic shock. however, the details of the underlying mechanisms remain unknown. in this study, we showed that intradermal treatment of mice with venom rhodocytin from the malayan viper calloselasma rhodostoma induced plasma extravasation, dependent on c-type lectin-like receptor ... | 2019 | 31685912 |
| antivenom neutralization of coagulopathic snake venom toxins assessed by bioactivity profiling using nanofractionation analytics. | venomous snakebite is one of the world's most lethal neglected tropical diseases. animal-derived antivenoms are the only standardized specific therapies currently available for treating snakebite envenoming, but due to venom variation, often this treatment is not effective in counteracting all clinical symptoms caused by the multitude of injected toxins. in this study, the coagulopathic toxicities of venoms from the medically relevant snake species bothropsasper, calloselasmarhodostoma, deinagki ... | 2020 | 31963329 |
| development of a generic high-throughput screening assay for profiling snake venom protease activity after high-resolution chromatographic fractionation. | snakebites cause upwards of 1.8 million envenomings, 138,000 deaths and 500,000 cases of long term morbidity each year. viper snake venoms (family viperidae) generally contain a high proportion of proteases which can cause devastating effects such as hemorrhage, coagulopathy, edema, necrosis, and severe pain, in envenomed victims. in this study, analytical techniques were combined with enzymatic assays to develop a novel method for the detection of snake venom protease activity by using rhodamin ... | 2020 | 32112787 |
| evolutionary interpretations of nicotinic acetylcholine receptor targeting venom effects by a clade of asian viperidae snakes. | ecological variability among closely related species provides an opportunity for evolutionary comparative studies. therefore, to investigate the origin and evolution of neurotoxicity in asian viperid snakes, we tested the venoms of azemiops feae, calloselasma rhodostoma, deinagkistrodon acutus, tropidolaeums subannulatus, and t. wagleri for their relative specificity and potency upon the amphibian, lizard, bird, rodent, and human α-1 (neuromuscular) nicotinic acetylcholine receptors. we utilised ... | 2020 | 32394055 |
| varespladib inhibits the phospholipase a2 and coagulopathic activities of venom components from hemotoxic snakes. | phospholipase a2 (pla2) enzymes are important toxins found in many snake venoms, and they can exhibit a variety of toxic activities including causing hemolysis and/or anticoagulation. in this study, the inhibiting effects of the small molecule pla2 inhibitor varespladib on snake venom pla2s was investigated by nanofractionation analytics, which combined chromatography, mass spectrometry (ms), and bioassays. the venoms of the medically important snake species bothrops asper, calloselasma rhodosto ... | 2020 | 32560391 |
| cytosolic phospholipase a2-α participates in lipid body formation and pge2 release in human neutrophils stimulated with an l-amino acid oxidase from calloselasma rhodostoma venom. | cr-laao, an l-amino acid oxidase isolated from calloselasma rhodosthoma snake venom, has been demonstrated as a potent stimulus for neutrophil activation and inflammatory mediator production. however, the mechanisms involved in cr-laao induced neutrophil activation has not been well characterized. here we investigated the mechanisms involved in cr-laao-induced lipid body (also known as lipid droplet) biogenesis and eicosanoid formation in human neutrophils. using microarray analysis, we show for ... | 2020 | 32620771 |
| sensitivity and specificity of 20-minute whole blood clotting test, prothrombin time, activated partial thromboplastin time tests in diagnosis of defibrination following malayan pit viper envenoming. | malayan pit vipers (calloselasma rhodostoma) are major health hazard in southern thailand causing systemic bleeding by defibrination and thrombocytopenia. twenty minute whole blood clotting test (20wbct) is a useful and informative bedside test recommended by who for diagnosis of significant coagulopathy following snakebite envenoming since it to some extent predicts the need for anti-venom therapy. prothrombin time (pt), international normalized ratio (inr) and activated partial thromboplastin ... | 2020 | 32712023 |
| neutralising effects of small molecule toxin inhibitors on nanofractionated coagulopathic crotalinae snake venoms. | repurposing small molecule drugs and drug candidates is considered as a promising approach to revolutionise the treatment of snakebite envenoming. in this study, we investigated the inhibiting effects of the small molecules varespladib (nonspecific phospholipase a2 inhibitor), marimastat (broad spectrum matrix metalloprotease inhibitor) and dimercaprol (metal ion chelator) against coagulopathic toxins found in crotalinae (pit vipers) snake venoms. venoms from bothrops asper, bothrops jararaca, c ... | 2020 | 33163338 |