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susceptibility to insect growth regulators and cuticle deposition of the cat flea (siphonaptera: pulicidae) as a function of age.wandering larval, pharate pupal, pupal, and pharate adult stages of the cat flea, ctenocephalides felis (bouché), were identified by examination of cuticular cross sections via scanning electron microscopy. visible morphological characteristics of each stage were identified and described. various stages of the flea throughout metamorphosis were exposed to juvenile hormone mimics and insect developmental inhibitors. wandering larvae treated with juvenile hormone mimics were unable to pupate, wher ...199910593081
dose titration of an injectable formulation of lufenuron in cats experimentally infested with fleas.to identify the lowest single dose of lufenuron injected s.c. that results in a 90% disruption of the flea (ctenocephalides felis) life cycle for 6 months in cats.199910622160
inhibition of immature ctenocephalides felis felis (siphonaptera: pulicidae) development in the immediate environment of cats treated with imidacloprid.the hypothesis that dermally applied imidacloprid may transfer from treated cats, felis catus l., to their immediate environment in quantities sufficient to have a significant effect on developing immature cat fleas, ctenocephalides felis (bouché), was tested in a controlled experiment. flea eggs harvested from untreated donor cats were incubated on replicated samples from blankets used by treated or untreated cats under standardized conditions. as compared with controls, the percentage of adult ...200010730492
a primary animal health care approach to treatment and control of flea (ctenocephalides felis) infestation in indigenous goats kept on communal grazing.this paper describes a primary health care approach to an infestation of indigenous goats by the common cat flea, ctenocephalides felis. the flea species was identified using scanning electron microscopy. the infested goats were kept on communal grazing at winterveld in the north-west province. they were penned at night in housing made of wire and corrugated iron. the owner complained that the goats were lethargic. fleas were found on the goats and flea larvae were found in the kraal. haematolog ...199910855818
[notes on fleas (siphonaptera) of the fox cerdocyon thous (canidae) from an endemic area of visceral leishmaniasis in jacobina, bahia, brazil].from july to september of 1998, 152 fleas were collected from 18 specimens of the fox cerdocyon thous captured in the visceral leishmaniasis endemic area of jacobina, state of bahia, brazil. the fleas were identified as: 136 rhopalopsyllus lutzi lutzi, 11 pulex irritans, 2 ctenocephalides canis, 1 ctenocephalides felis felis and 2 xenopsylla cheopis.200010881126
effect of pyriproxyfen in the blood diet of cat fleas on adult survival, egg viability, and larval development.the activity of pyriproxyfen in the blood diet was investigated for its efficacy against adult, egg, and larval stages of the cat flea, ctenocephalides felis (bouché). adult fleas were housed in plastic cages and fed treated bovine blood using an artificial membrane system that allows fleas to feed ad libitum through a parafilm membrane. control fleas received blood without pyriproxyfen. results showed that ingested pyriproxyfen was relatively nontoxic to adult fleas over a period of 10 d at con ...200010916290
development of an assay for the screening of compounds against larvae of the cat flea (siphonaptera: pulicidae).larvae of the cat flea, ctenocephalides felis (bouché), are the target of numerous growth regulators. this study explores the development of an assay that tests the susceptibility of cat flea larvae to a wide range of compounds. different rearing media and containment units were tested that would facilitate optimization. larvae of various ages were compared, and 7-d-old larvae were found to be optimal because they were the most uniform in size and age and exhibited a need to feed. the assay coul ...200010916298
first bloodmeal of ctenocephalides felis felis (siphonaptera: pulicidae) on cats: time to initiation and duration of feeding.three experiments were conducted on cats to evaluate precocity and duration of the first blood meal of ctenocephalides felis felis (bouché). percentage of engorged fleas was calculated for fleas held on cats for 5, 15, 30, and 60 min. duration of first blood meal was also measured for individual fleas confined on cats. when fleas are free in the hair coat, 24.9% are engorged after 5 min and 97.2% are engorged after 1 h. fleas confined to a vial on the cats fed significantly sooner; 60% of female ...200010916307
the effects of flea egg consumption on larval cat flea (siphonaptera: pulicidae) development.cat flea larvae feeding on the feces of adult fleas that were maintained on cats and were provided with frozen flea eggs ad libitum each consumed an average of 21.7 +/- 3.9 eggs and developed rapidly with 100% adult emergence. in contrast, 93.4% of larvae held individually and provided with only flea feces did not survive to the adult stage. developing larvae consumed eggs in the presence of yeast and rearing diet. in a second experiment, larvae provided with flea feces and eggs and maintained a ...200010925802
infection and seroconversion of cats exposed to cat fleas (ctenocephalides felis bouché) infected with rickettsia felis.cats with no prior exposure to cat fleas were exposed to fleas infected with rickettsia felis and monitored monthly for seroconversion via an indirect fluorescent assay (ifa). each of 12 cats continually infested with fleas, seroconverted by four months post-exposure. three of six cats fed on by 50 fleas for 15 minutes once per week also seroconverted by month four. rickettsia felis dna was detected by pcr in the blood of five of the 16 cats.200010925806
dose selection of selamectin for efficacy against adult fleas (ctenocephalides felis felis) on dogs and cats.selamectin, a novel avermectin, was evaluated in two controlled studies (one in beagles, one in domestic shorthaired cats) to determine an appropriate topical dose for efficacy against adult ctenocephalides felis felis (c. felis) fleas on dogs and cats for 1 month. for each study, animals were allocated randomly to four treatments. one treatment consisted of the inert formulation ingredients (vehicle) administered as a negative control, and the other three treatments consisted of a single topica ...200010940520
efficacy of selamectin against adult flea infestations (ctenocephalides felis felis and ctenocephalides canis) on dogs and cats.selamectin was evaluated in eight controlled studies (4 in dogs, 4 in cats) to determine the efficacy of a single topical unit dose providing the recommended minimum dosage of 6mgkg(-1) against ctenocephalides felis felis and ctenocephalides canis fleas on dogs and against c. felis on cats. in addition, the effect of bathing on the efficacy of selamectin against c. felis was evaluated. identical studies were performed in beagles and domestic shorthaired cats. for each study, animals were allocat ...200010940521
evaluation of the effects of selamectin against adult and immature stages of fleas (ctenocephalides felis felis) on dogs and cats.the adulticidal, ovicidal, and larvicidal effects of selamectin against flea (ctenocephalides felis felis) infestations on dogs and cats were evaluated in a series of seven controlled and masked studies (three in cats, four in dogs). animals were randomly allocated to treatment with either selamectin at a minimum dosage of 6mgkg(-1) in the commercial formulation or one of two negative-controls (0.9% nacl solution or the vehicle from the commercial formulation). treatments were administered topic ...200010940522
efficacy of selamectin in the treatment and prevention of flea (ctenocephalides felis felis) infestations on dogs and cats housed in simulated home environments.the efficacy of selamectin, a novel avermectin, in protecting dogs and cats against experimentally induced environmental flea (ctenocephalides felis felis) infestations, was evaluated in a series of controlled and masked studies. purpose-bred shorthaired cats and beagles were randomly allocated to treatment with either selamectin at a minimum dosage of 6mgkg(-1) of body weight in the commercial formulation or the negative control treatment (vehicle only), and housed in controlled simulated home ...200010940523
efficacy of selamectin administered topically to pregnant and lactating female dogs in the treatment and prevention of adult roundworm (toxocara canis) infections and flea (ctenocephalides felis felis) infestations in the dams and their pups.the efficacy of selamectin in the treatment and prevention of naturally acquired toxocara canis infections and experimentally induced flea (ctenocephalides felis felis) infestations in dams and their suckling pups was evaluated by administering selamectin to the adult females only, approximately 40 and 10 days before parturition and 10 and 40 days after parturition. unit doses of the commercial formulation of selamectin were administered to the dams to provide at least the minimum recommended do ...200010940534
a comparison of jump performances of the dog flea, ctenocephalides canis (curtis, 1826) and the cat flea, ctenocephalides felis felis (bouché, 1835).jump performances of ctenocephalides canis and ctenocephalides felis felis have been measured and compared on unfed young imagos. the mean length of the c. felis felis jump was 19.9+/-9.1cm; minimum jump was 2cm, and the maximum was one 48cm. the c. canis jump was significantly longer (30.4+/-9.1cm; from 3 to 50cm). for height jump evaluation, grey plastic cylindric tubes measuring 9cm in diameter were used. their height was increasing from 1 to 30cm by 1cm. groups of 10 fleas of the same specie ...200010962162
pupation site selection of cat fleas (siphonaptera: pulicidae) in various carpet types and its influence on insecticide efficacy.pupation sites of cat flea, ctenocephalides felis (bouché), larvae were determined in three styles of nylon and one style of wool carpet. nylon saxony carpet had 59.3% of pupae at the top of the pile and 40% at the base of the pile. in nylon contract carpet, 55.2% of pupae were found at the top, 42.6% in the middle, and only 2.2% at the base of the pile. nylon loop carpet contained 59.2% of pupae at the base, 25.5% in the middle, and 15.3% in the top of the pile. wool loop carpet had 92.4% at th ...200010985057
evaluation of the catandog's tag to prevent flea infestations, inhibit flea reproduction or repel existing flea infestations on cats.to evaluate the ability of the catandog's tag to eliminate fleas, inhibit egg production and prevent flea infestations, six domestic shorthaired cats were randomly allocated to two treatment groups and housed individually in stainless steel metabolic cages. three cats were each fitted with a catandog's tag; the other three cats were not fitted with tags and served as controls. following a 42-day acclimation period, each of the six cats was infested with 100, 1-3 day post-emergence, adult ctenoce ...200010996742
avermectins and flea control: structure-activity relationships and the selection of selamectin for development as an endectocide for companion animals.evaluation of a wide range of avermectin derivatives for flea activity in an in vitro feeding screen using the cat flea, ctenocephalides felis, revealed a narrow structure-activity relationship (sar) with activity surprisingly associated with monosaccharides and especially their c-5-oximes. we discovered commercially exploitable flea activity in a single compound, selamectin 33, which also possessed the necessary antiparasitic spectrum and margin of safety for development as a broad-spectrum com ...200011003146
identification, cloning, and characterization of a major cat flea salivary allergen (cte f 1).an 18 kda protein isolated from saliva of the cat flea, ctenocephalides felis, elicits a positive intradermal skin test (idst) in 100 and 80% of experimental and clinical flea allergic dogs, respectively. using solid-phase enzyme-linked immuno assay (elisa), this protein detected ige in 100 and 80% of experimental and clinical flea allergic dogs, respectively. a cdna (pfsi) encoding a full-length cte f 1 protein was isolated from a c. felis salivary gland cdna library, using a combination of pcr ...200011074254
recent advances in diagnosis and treatment of cat scratch disease.the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ...200011095849
evaluation of efficacy of selamectin and fipronil against ctenocephalides felis in cats.to evaluate efficacy of monthly administration of selamectin and fipronil against ctenocephalides felis in cats.200011110456
evaluation of efficacy of selamectin, fipronil, and imidacloprid against ctenocephalides felis in dogs.to evaluate efficacy of monthly administration of selamectin, fipronil, and imidacloprid against ctenocephalides felis in dogs.200011110457
effects of multiple mating on female reproductive output in the cat flea (siphonaptera: pulicidae).multiple mating behavior of female cat fleas, ctenocephalides felis (bouché), was confirmed in this study, and its effects on fecundity and fertility were investigated as well. the number of fertile eggs produced by mated females was close to nil within 7 d after removal of males, but it was resumed when females were exposed to males again on day 7. multiple-mated females displayed significantly higher fecundity (400.3 eggs per female) and fertility (182.8 viable eggs per female) than single-mat ...200011126536
preliminary characterisation of esterase and platelet-activating factor (paf)-acetylhydrolase activities from cat flea (ctenocephalides felis) salivary glands.naphthyl esterase and platelet-activating factor (paf)-acetylhydrolase activities were detected in the salivary glands of the cat flea, ctenocephalides felis. salivary naphthyl esterase activity is disgorged during exploratory probing. whole extracts of salivary glands contain esterase activity against the short-chain naphthyl esters alpha-naphthyl acetate (approximately 210pmol/min/gland pair; 10.0micromol/min/mg specific activity; k(m) approximately 59microm) and beta-naphthyl acetate (approxi ...200111164337
[the state of in vitro breeding of lice and fleas]laboratory breeding of lice, fleas and other bloodsucking arthropods is necessary for developing and testing the efficacy of new drugs and alternative arthropod control methods. they are necessary to investigate their role as vectors of pathogens and to develop methods for diagnosis and therapy of arthropod caused or transmitted diseases. to date, it is not possible to replace rabbits as natural blood sources for breeding lice colonies (pediculus spp.). but on the base of the knowledge about lic ...199611178453
cat-scratch disease.cat-scratch disease (csd) was first described by debré in 1950, yet the causative bacterial agent of csd remained obscure until 1992, when bartonella (formerly rochalimaea) henselae was implicated in csd by serological and microbiologic studies. bartonella henselae had initially been linked to bacillary angiomatosis (ba), a vascular proliferative disease most commonly associated with long-standing human immunodeficiency virus infection or other significant immunosuppression. bartonella henselae ...200011189710
control of immature stages of the flea ctenocephalides felis (bouché) in carpets exposed to cats treated with imidacloprid.fleas cause allergic dermatitis in cats and dogs and therefore warrant control. it has been demonstrated previously that there is marked inhibition of the development of the immature stages of the cat flea ctenocephalides felis on fleece blankets exposed to cats treated with imidacloprid. this study reports on the efficacy of imidacloprid in suppressing adult flea emergence in carpet exposed to treated cats. circular discs of carpet pre-seeded with flea eggs and larvae were exposed to 6 untreate ...200011212931
first blood meal of ctenocephalides canis (siphonaptera: pulicidae) on dogs: time to initiation of feeding and duration.two experiments were conducted on dogs to evaluate interval to initiation and duration of the first blood meal of ctenocephalides canis (curtis). percentage of fed male and female fleas was calculated for fleas held on dogs for 5, 15, 30, 60 min, 6, and 24 hr. duration of first blood meal was also measured for individual fleas confined on dogs. when fleas were free in the hair coat, 21.2% had begun blood feeding within 5 min. after 1 hr, 72.5% of fleas had fed. after 6 hr, 95.2% of males and 100 ...200111227894
seasonal occurrence of ctenocephalides felis felis and ctenocephalides canis (siphonaptera: pulicidae) infesting dogs and cats in an urban area in cuernavaca, mexico.the seasonal occurrence of ctenocephalides felis felis (bouché and ctenocephalides canis (curtis) infestation on dogs and cats in cuernavaca city in mexico, was determined by examining 1,803 dogs and 517 cats at two veterinary clinics during 1995-1997. the overall flea infestation was 30.3 and 30.1% for dogs and cats, respectively. there were no significant differences (p > 0.05) in percentage of infestation among years for both hosts. the infestation was somewhat higher in spring, summer, and a ...200111268680
effects of juvenile hormone on eggs and adults of the cat flea (siphonaptera: pulicidae).juvenile hormone iii plays a major role in regulating feeding and reproduction in the adult cat flea, ctenocephalides felis (bouché). both blood consumption and egg production increased in a dose-dependent manner up to a maximum at 1,250 ppm when fleas were continuously exposed to concentrations up to 12,500 ppm juvenile hormone. histological studies demonstrated that juvenile hormone iii also stimulated cellular differentiation of salivary gland epithelia, midgut epithelia, and fat body cells, ...200111268696
comparative study on the effects of three insecticides (fipronil, imidacloprid, selamectin) on developmental stages of the cat flea (ctenocephalides felis bouché 1835): a light and electron microscopic analysis of in vivo and in vitro experiments.the effects of three insecticides (fipronil, imidacloprid and selamectin) on developmental stages of cat fleas (ctenocephalides felis) were studied in vivo, in vitro and by means of light and electron microscopy. the results were documented by video. adult fleas were attached to the skin of dogs that had been treated 7 days before with one of the three compounds. furthermore, adult fleas were exposed exclusively to the hair and skin debris of such treated dogs or were placed on filter papers tha ...200111293567
topical application as a method for comparing the effectiveness of insecticides against cat flea (siphonaptera: pulicidae).a topical (micro-droplet) bioassay is described for the cat flea, ctenocephalides felis (bouche). key parameters are the short carbon dioxide anesthetization and the use of 0.1 ml of acetone per flea. the method was used to compare the effectiveness of 13 insecticides. ld95 values in nanograms per flea were nitenpyram 0.68, fipronil 0.69, deltamethrin 0.70, imidacloprid 0.81, cypermethrin 5.4, fenthion 8.0, diazinon 12, permethrin 19, malathion 29, bendiocarb 170, ddt 710, propoxur 1,300, carbar ...200111296822
control of flea populations in a simulated home environment model using lufenuron, imidacloprid or fipronil.control strategies were evaluated over a 6-month period in a home simulation model comprising a series of similar carpeted pens, housing matched groups of six cats, in which the life-cycle of the flea ctenocephalides felis felis bouche (siphonaptera: pulicidae) had been established. additional adult fleas were placed on the cats at intervals to mimic acquisition of extraneous fleas from outside the home. treatment strategies included a single subcutaneous deposition of injectable lufenuron suppo ...200111297105
systemic efficacy of nodulisporic acid against fleas on dogs.nodulisporic acid a (nsa) is a novel natural product from a new structural class that was shown previously to have insecticidal activity against blowfly larvae. to determine if there was useful systemic efficacy against fleas (ctenocephalides felis). nsa was evaluated in an artificial membrane flea feeding device and in dogs. in the artificial membrane flea feeding device, adult c. felis were allowed to feed on bovine blood containing various concentrations of nsa through a parafilm membrane. ns ...200111318575
rickettsia felis: molecular characterization of a new member of the spotted fever group.in this report, placement of rickettsia felis in the spotted fever group (sfg) rather than the typhus group (tg) of rickettsia is proposed. the organism, which was first observed in cat fleas (ctenocephalides felis) by electron microscopy, has not yet been reported to have been cultivated reproducibly, thereby limiting the standard rickettsial typing by serological means. to overcome this challenge, several genes were selected as targets to be utilized for the classification of r. felis. dna fro ...200111321078
recent developments in ectoparasiticides.the sales and use of ectoparasiticides for the control of arthropod parasites of domestic animals constitute a major sector of the global animal health market. animals are infected by a number of parasitic insect and acarine species causing major economic losses in production livestock, intense irritation and skin disease in companion animals, or public health issues, including bites of humans or zoonotic disease transmission. dog and cat fleas, for example, can be a serious source of both anima ...200111352483
off-host observations of mating and postmating behaviors in the cat flea (siphonaptera: pulicidae).a blood meal was necessary for a male cat flea, ctenocephalides felis (bouché), to display the mating attempts to an unfed or fed female. more mating pairs resulted when both sexes were fed. copulation occurred when fed fleas were placed on surfaces with temperatures from 34 to 42 degrees c. this article not only describes the mating and postmating behaviors of cat fleas, but also compares them with those of other fleas. the sequence of mating behavior began when a male approached a female ventr ...200111372958
prevalence of vectors of the spotted fever group rickettsiae and murine typhus in a bedouin town in israel.a survey of the vectors of spotted fever group rickettsiae and of murine typhus was carried out in rahat, a bedouin town in the negev desert, where the diseases are endemic. houses with known cases of spotted fever group rickettsiae or murine typhus were compared with those without reported clinical cases. a neighboring jewish community, lehavim, where no cases of spotted fever group rickettsiae and murine typhus were reported in recent years, was used as a control. in the houses of patients wit ...200111372975
species of flea (siphonaptera) infesting pets and hedgehogs in germany.the species of flea infesting pets and hedgehogs in germany were investigated through a survey of small animal practitioners throughout the country who were asked to collect specimens at their veterinary practices. a total of 625 veterinarians/veterinary practices responded and provided 2445 intact anti identifiable flea specimens. these fleas originated from 294 dogs (795 fleas), 334 cats (1152 fleas), 76 hedgehogs (481 fleas), five domestic rabbits (10 fleas), one golden hamster (four fleas) a ...200111393815
evaluation of imidacloprid for the treatment and prevention of cat flea (ctenocephalides felis felis) infestations on rabbits. 200111425257
cat flea (ctenocephalides felis) infestation in quarantined marsh deer (blastocerus dichotomus) populations.marsh deer (blastocerus dichotomus) were captured for a research program in brazil and maintained in quarantine stations. after 60 days, fleas were detected on animals and identified as ctenocephalides felis felis. elimination of the infestation was difficult. animal treatment with a fipronil-based compound was effective, and subsequently captured animals were treated prophylactically. some animals remained infested, and some died from the infestation.200011428409
establishment of the cat flea (ctenocephalides felis felis) on the ferret (mustela putorius furo) and its control with imidacloprid.as the ferret, mustela putorius furo l. (carnivora: mustelidae), is becoming increasingly popular as a pet animal and as it is susceptible to the cat-flea, ctenocephalides felis felis bouché (siphonaptera: pulicidae), an experimental model was established for evaluating insecticidal treatments on this host. a high establishment rate (76.7-91.8%) was recorded when 60 unfed adult c. felis were placed on ferrets. this provided an adequate infestation for chemotherapeutic evaluation without causing ...200111434557
cat-scratch disease: epidemiology, aetiology and treatment.cat-scratch disease (csd) is a clinical syndrome that usually presents as a self-limiting lymphadenopathy associated with a cat scratch or bite. commonly affecting children and young adults, it has a worldwide distribution. in temperate climates, higher rates are reported in the autumn and winter, which can be attributed to the seasonal breeding of the domestic cat. the organism responsible was identified in 1983, having eluded detection for 50 years. initially, afipia felis was thought to be th ...200111440202
systemic activity of the avermectins against the cat flea (siphonaptera: pulicidae).ivermectin has potent systemic activity against numerous species of nematodes and arthropods, but there are some important species in these two groups, such as the cat flea, ctenocephalides felis (bouché), that appear to be refractory to it. in an effort to determine if the lack of systemic activity against c. felis is specific to ivermectin, or if it is a class-wide phenomenon, 20 avermectin derivatives were tested in an artificial membrane flea feeding system at concentrations of 20, 10, and 1 ...200111476339
accumulation and persistence of flea larvicidal activity in the immediate environment of cats treated with imidacloprid.to investigate the persistence of flea larvicidal activity in the immediate environment of cats treated with imidacloprid, eggs of the cat flea ctenocephalides felis felis bouché (siphonaptera: pulicidae), from untreated donor cats, were incubated on samples of fleece blanket taken from the floor of cages used by treated or untreated cats for a total of 10 or 20 6-h periods over 2-4 weeks, respectively. sufficient imidacloprid accumulated during these periods to reduce the emergence of adult fle ...200111583454
systemic efficacy of nodulisporamides against fleas on dogs.nodulisporic acid a (nsa) has been shown previously to be safe in dogs and to deliver >90% flea control for 4 days following a single oral administration. three newly prepared nodulisporamide derivatives were subsequently identified from an artificial membrane flea feeding system as exhibiting potency substantially greater than nsa. to determine if they have superior in vivo activity, these 3 nodulisporamides, as well as nsa, were evaluated in dogs at 15 mg/kg/os. parasite challenges were made b ...200111695382
effect of 0.29% w/w fipronil spray on adult flea mortality and egg production of three different cat flea, ctenocephalides felis (bouché), strains infesting cats.to evaluate the effect of fipronil spray on adult flea mortality and flea egg production of three different cat flea, ctenocephalides felis (bouché) strains, 30 domestic short hair cats were randomly allocated into six groups of five cats each. on day 0, cats in groups 2, 4 and 6 were treated with fipronil at 5-6ml/kg. cats in groups 1, 3 and 5 served as untreated controls. on days -2, 7, 14, 21, and 28 each cat was infested with 50 adult cat fleas. groups 1 and 2 were infested with fleas from t ...200111731076
presence of rickettsia felis in the cat flea from southwestern europe.rickettsia felis, formerly called elb agent, was identified by using molecular biology techniques in the cat flea (ctenocephalides felis felis) from southwestern spain. for the first time this flea-transmitted rickettsia has been detected within its vector in eurasia.200211749758
comparison of the activity of selamectin, imidacloprid and fipronil for the treatment of dogs infested experimentally with ctenocephalides canis and ctenocephalides felis felis.twenty-four beagles were randomly allocated into four groups of six and housed in separate cages. each dog was infested with 25 ctenocephalides canis and 25 ctenocephalides felis felis and two days later (day 0) the dogs in groups 1, 2 and 3 received a spot-on application of selamectin (6 mg/kg), imidacloprid (10 mg/kg), or fipronil (6-7 mg/kg), respectively, while the dogs in group 4 were not treated. the dogs were combed 48 hours later, the fleas were removed, counted and their species were de ...200111787782
development of a mouse model to determine the systemic activity of potential flea-control compounds.probe studies were performed to determine if the cat flea (ctenocephalides felis), the most common ectoparasite of companion animals, will feed on laboratory mice and, if so, to incorporate this into a small animal assay to detect systemically active compounds. consequently, a protocol was developed which incorporated acepromazine maleate to temporarily sedate various strains of mice and allow fleas a window of time to feed undisturbed. for validation of the model, cd-1 mice were dosed per os wi ...200211812623
effect of diet composition on weight gain, sperm transfer, and insemination in the cat flea (siphonaptera: pulicidae).weight gain by adult cat fleas, ctenocephalidesfelis (bouché), was influenced primarily by the concentrations of protein and sodium chloride in the feeding solution. after 48 h of feeding, fleas fed whole blood weighed almost twice as much as fleas fed plasma or hemolyzed blood and 1.25 times as much as fleas fed 0.15 m sodium chloride. when fleas were fed sodium chloride solutions ranging from 0 to 0.5 m, weight gain was greatest on the 0.15- or 0.2-m solutions. weight gain decreased significan ...200211931038
laboratory evaluation of fipronil and imidacloprid topical insecticides for control of the plague vector oropsylla montana (siphonaptera: ceratophyllidae) on california ground squirrels (rodentia: sciuridae).two insecticides, fipronil and imidacloprid, were evaluated for efficacy and longevity against oropsylla montana (baker), the most important vector of plague in california. wild-caught california ground squirrels, spermophilus beecheyi (richardson), were individually housed in the laboratory to serve as natural hosts to o. montana and for on-animal insecticide trials. several concentrations oftechnical grade fipronil and imidacloprid in acetone were applied to samples of clean rodent bedding to ...200211931250
flea species from dogs in three cities of chile.the purpose of this study was to establish the pattern of flea infestation in dogs in the cities of santiago (33 degrees latitude south, 70 degrees longitude west), concepción (36 degrees latitude south, 73 degrees longitude west) and osorno (40 degrees latitude south, 73 degrees longitude west), located in different parts of chile. the only species of fleas found on dogs from the three cities were ctenocephalides felis felis (41.8%), ctenocephalides canis (39.4%) and pulex irritans (18.8%). alt ...200211934466
prevalence of antibodies to bartonella henselae, b. elizabethae and b. quintana in swedish domestic cats.sera from 292 cats were analyzed by means of indirect immunofluorescence for antibodies to bartonella henselae, b. quintana and b. elizabethae. the sera were sent to the swedish national institute of veterinary medicine for health monitoring and were tested retrospectively for antibodies to bartonella. the most prevalent antibodies (25%) reacted with the b. elizabethae antigen. cats with such antibodies were older than those without antibodies. the prevalence of antibodies to b. elizabethae was ...200212030392
evaluation of the comparative efficacy of selamectin against flea (ctenocephalides felis felis) infestations on dogs and cats in simulated home environments.the comparative efficacy of monthly administration of selamectin or lufenuron against ctenocephalides felis felis on dogs and cats was evaluated over a 5-month period in flea-infested environments. twenty-four dogs and 32 cats were randomly allocated to receiving a topical treatment with selamectin or an oral administration of tablets containing lufenuron/milbemycin oxime (for dogs) or lufenuron only (for cats). each product was administered in accordance with the manufacturer's label recommenda ...200212031818
control of ctenocephalides felis on dogs and cats using the insect growth regulator (or chitin synthesis inhibitor) lufenuron program, in egypt.lufenuron, the chitin synthesis inhibitor (program, novartis-switzerland) was given orally at doses of 10-mg/kg b/w to dogs and 30-mg/kg b/w to cats every four weeks (monthly) for the treatment of experimental flea infestations. three to four weeks after the last infestation, lufenuron had effectively controlled the ct. felis infestation of dogs and cats as the drug prevented the development of the offspring of adult female fleas feeding on animals. flea populations were absent or very low and r ...200212049275
therapy and prevention of parasitic insects in veterinary medicine using imidacloprid.ectoparasitic insects play a major role in veterinary medicine. the flea, especially the cat flea (ctenocephalides felis felis bouch 1835) is the most important ectoparasite worldwide. the cat flea parasitizes not only on dogs and cats but also on other warm-blooded animals including humans. the veterinary importance of flea infestation are dermatological conditions due to allergic reactions to antigens in the flea saliva and the transmission of infectious agents like bacteria, viruses and helm ...200212052186
comparison of nodulisporic acid analogs in a lucilia sericata in vitro assay and a ctenocephalides felis membrane feeding system.a medicinal chemistry program on the nodulisporic acid chemical class, guided by an artificial membrane flea-feeding assay, has recently identified permissive and nonpermissive regions of the pharmacophore for exploitation against fleas. this pathway was validated when several promising compounds from this program were administered orally to dogs at 15.0 mg/kg and found to have >90% flea activity for 2 wk. to determine if a surrogate insect assay would have provided the same guidance, a nodulisp ...200212053989
factors influencing sperm transfer and insemination in cat fleas (siphonaptera: pulicidae) fed on an artificial membrane system.sperm transfer through the epididymis, a prerequisite for insemination of cat fleas, ctenocephalides felis (bouché) was stimulated by exposure of unfed male fleas to juvenile hormone iii residues for 3 d at 25 degrees c or exposure of unfed fleas to 37 degrees c for 6 d. sperm transfer was completed at least three times faster in unfed males held at 37 degrees c than in those held at 25 degrees c. although percentage sperm transfer in fleas fed water or 0.15 m saline at 37 degrees c was not sign ...200212061443
ownership of cats or dogs does not increase exposure to rickettsia typhi.in the united states, murine typhus (rickettsia typhi) is endemic in southern california and south texas. evidence indicates the natural host/vector system is the opossum and cat flea, the most common flea on both cats and dogs, and speculation suggests that pet contact may pose a risk for r typhi exposure. serum from 140 subjects was tested for rickettsial antibodies by an enzyme-linked immunosorbent assay that used an r typhi antigen. each subject was asked about pet contact status and placed ...200212073903
lack of correlation between bartonella dna detection within fleas, serological results, and results of blood culture in a bartonella-infected stray cat population.to correlate the presence of different bartonella species in the blood of a stray cat population trapped on a french military base with specific antibodies and species detected in cat fleas.200212084102
cloning, partial purification and in vivo developmental profile of expression of the juvenile hormone epoxide hydrolase of ctenocephalides felis.cdnas encoding two different epoxide hydrolases (ncfeh1 and ncfeh2) were cloned from a cdna library prepared from the wandering larval stage of the cat flea, ctenocephalides felis. predicted translations of the open reading frames indicated the clones encoded proteins of 464 (cfeh1) and 465 (cfeh2) amino acids. these proteins have a predicted molecular weight of 53 kda and a putative 22 amino acid n-terminal hydrophobic membrane anchor. the amino acid sequences are 77% identical, and both are ho ...200212125060
the effects of diet upon pupal development and cocoon formation by the cat flea (siphonaptera: pulicidae).cocoon formation by cat flea larvae was directly related to the quantity of eggs or yeast consumed. larvae consuming either 1-3 eggs or 0.25-1.0 mg of yeast developed as naked pupae or formed incomplete cocoons. third instar cat flea larvae fed upon naked pupae and pupae within partial cocoons, but complete cocoons protected pupae from cannibalism. first and second instars did not attack pupae. when larvae were provided with a carpet fiber for protection, a greater number of fleas successfully d ...200212125871
vertical transmission of rickettsia felis in the cat flea (ctenocephalides felis bouché).rickettsia felis can be maintained in cat fleas by vertical transmission for up to 12 generations without the benefit of an infected host. horizontal transmission or the acquisition of r. felis by fleas feeding on cats or artificially infected meals were not demonstrated in this study. horizontal transmission of r. felis by the ingestion of feces or eggs by flea larvae was not detected. we also tested for and found no evidence to support horizontal transmission by contact among positive fleas an ...200212125879
development of a larval bioassay for susceptibility of cat fleas (siphonaptera: pulicidae) to imidacloprid.strategies for controlling cat fleas, ctenocephalidesfelisfelis (bouché), have undergone dramatic changes in the past 5 yr. with the advent of on-animal treatments with residual activity the potential for the development of insecticide resistance increases. a larval bioassay was developed to determine the baseline susceptibility of field-collected strains of cat fleas to imidacloprid. all four laboratory strains tested showed a similar level of susceptibility to imidacloprid. advantages of this ...200212144302
distribution of cat fleas (siphonaptera: pulicidae) on the cat.a total of 3,382 cat fleas, ctenocephalidesfelis (bouche), was taken from 164 of the 200 stray cats examined. it was observed that cat fleas preferred specific areas on the cat. a significantly higher mean number of fleas was found on the area of head plus neck than on the ventral part of the body. more specifically, the mean number of fleas was highest on both of the neck and dorsal areas. however, in terms of the density of fleas, the neck had more fleas than the dorsal area did. the fewest fl ...200212144305
analysis of expressed sequence tags from subtracted and unsubtracted ctenocephalides felis hindgut and malpighian tubule cdna libraries.insect hindgut and malpighian tubule (hmt) tissues regulate the contents of the haemolymph through the excretion of waste products and the specific reabsorption of nutrients. as such, they perform a role that is essential for survival and may contain molecular targets for insect control strategies. in order to discover genes expressed in the hmt tissues of the cat flea, ctenocephalides felis, expressed sequence tags (ests) were generated from an unsubtracted hmt cdna library and from a subtracte ...200212144694
insecticide susceptibilities of cat fleas (siphonaptera: pulicidae) from several regions of the united states.eleven cat flea, ctenocephalides felis (bouchè), strains, seven field-collected and four laboratory-colonized, were assayed for susceptibilities to five insecticides (carbaryl, chlorpyrifos, malathion, permethrin, and pyrethrin) with an insecticide-treated, horizontally-oriented, nylon 6,6 disk in a test tube. the pyrethrin was synergized using piperonyl butoxide (pbo). flea mortality at two doses was recorded after 4 and 24 h exposures. the field strains from texas and florida tolerated carbary ...200212349857
prevalence of ectoparasites in a population of feral cats from north central florida during the summer.ectoparasites are a common and important cause of skin disorders in cats. ectoparasites are capable of disease transmission and can cause life-threatening anemia in young or debilitated animals. the objective of this study was to determine the potential feline ectoparasites in domestic cats by using a cohort of feral cats from north central florida that have not received veterinary care and have no known exposure to insecticide application. a total of 200 feral cats were randomly selected for th ...200212383632
identification, characterization, and cloning of an immunoglobulin degrading enzyme in the cat flea, ctenocephalides felis.the degradation of cat immunoglobulin g (igg) in blood-fed adult c. felis midguts was examined. sds-page analysis of dissected midgut extracts obtained from c. felis that had been blood fed for various times between 0 to 44 h revealed that by 24 h most of the high molecular weight proteins, including the heavy chain of igg, were digested. a 31-kda serine protease with igg degrading activity was purified from fed c. felis midguts by benzamidine affinity chromatography, hydrophobic interaction chr ...200212386841
efficacy of two 65 % permethrin spot-on formulations against canine infestations of ctenocephalides felis and rhipicephalus sanguineus.the efficacy of two formulations of a topically applied 65% permethrin spot-on for dogs (defend exspot treatment for dogs, schering-plough animal health corp.) was evaluated against experimental infestations of the cat flea, ctenocephalides felis, and the brown dog tick, rhipicephalus sanguineus. thirty dogs were randomly allocated to treatment with 65 % permethrin in diethylene glycol monomethyl ether (original formulation), 65 % permethrin in propylene glycol monomethyl ether (test formulation ...200212447841
household papular urticaria.papular urticaria often occurs after bites of insects such as mosquitoes, sandflies, bed bugs and fleas. multiple bites and local pruritus are characteristic symptoms. treatment is usually symptomatic and includes antihistamines and corticosteroids. the reappearance of the symptoms can be prevented by successful control of the parasite.200212455178
comparative evaluation of juvenoids for control of cat fleas (siphonaptera: pulicidae) in topsoil.the efficacy of a photostable formulation of methoprene and two photostable juvenoids, fenoxycarb and pyriproxyfen, and their residual activity in inhibiting the emergence of adult cat fleas, ctenocephalides felis (bouché), was studied in topsoil. nursery pots composed of clay, peat, and plastic, and wooden flats were used to hold soil samples. treated soil samples were exposed to sunlight during the 63-d study period. methoprene was as effective as fenoxycarb and pyriproxyfen against cat fleas ...200212495188
studies on the growth of bartonella henselae in the cat flea (siphonaptera: pulicidae).two out of three pools of cat fleas, ctenocephalides felis (bouche), that were fed bartonella henselae-positive cat blood for 3 d and then bovine blood for 3 d, were polymerase chain reaction (pcr) positive for b. henselae. in a second experiment, three cats were inoculated with a streptomycin-resistant strain of b. henselae. after the cats were inoculated, caged cat fleas were fed on the cats during three different periods, and then pooled and transferred to noninfected recipient cats. in the f ...200212495192
emended description of rickettsia felis (bouyer et al. 2001), a temperature-dependent cultured bacterium.on the basis of phenotypic data obtained on the strain marseille-urrwfxcal2(t), isolated from the cat flea ctenocephalides felis, the description of rickettsia felis (bouyer et al., 2001) is emended and marseille-urrwfxcal2(t) is proposed as the type strain of the species. on the basis of polyphasic characterization, especially the inability to grow at temperatures higher than 32 degrees c on vero cells that allow growth of other rickettsia to at least 35 degrees c, it is confirmed that this age ...200212508865
consumption of flea faeces and eggs by larvae of the cat flea, ctenocephalides felis.the effects of the consumption of flea faeces and non-viable eggs on larval development in the cat flea ctenocephalides felis (bouché) (siphonaptera: pulicidae) were investigated. only 13.3% of larvae developed into adults when fed a diet of male or female flea faeces alone; however, 90% of larvae developed into adults when fed on flea faeces supplemented with non-viable flea eggs. when fed with non-viable eggs alone, larvae did not develop into adults. nevertheless, non-viable eggs may provide ...200212510898
efficacy of three dose volumes of topically applied 65% permethrin against ctenocephalides felis and rhipicephalus sanguineus on dogs weighing 30 kg or more.twenty-five dogs were evenly and randomly allocated by weight to five treatment groups: untreated control, 2 ml 65% permethrin (dogs weighing 15 to 29 kg, "average dogs"), and 2, 3, or 4 ml 65% permethrin (dogs weighing > or = 30 kg, "big dogs"). each dog was infested with 125 unfed, adult cat fleas, ctenocephalides felis, and 50 unfed, adult brown dog ticks, rhipicephalus sanguineus, on days -3 (ticks only), -2 (fleas only), 3, 7, 14, 17, 21, 24, and 28. fleas and ticks were counted 1 and 3 or ...200212584681
comparative speed of kill between nitenpyram, fipronil, imidacloprid, selamectin and cythioate against adult ctenocephalides felis (bouché) on cats and dogs.speed of kill and percentage kill of nitenpyram (capstar) was compared to fipronil (frontline) spot-on), imidacloprid (bayvantage/advantage), selamectin (stronghold/revolution) and cythioate (cyflee) against adult fleas on cats and dogs 3 and 8h post-treatment. selamectin was used on dogs only; cythioate was used on cats only. groups of eight cats and eight dogs (four males and four females each) were experimentally infested with 100 unfed fleas 1 day prior to treatment with the test products. o ...200312591200
molecular detection of bartonella quintana, b. koehlerae, b. henselae, b. clarridgeiae, rickettsia felis, and wolbachia pipientis in cat fleas, france.the prevalences of bartonella, rickettsia, and wolbachia were investigated in 309 cat fleas from france by polymerase chain reaction (pcr) assay and sequencing with primers derived from the glta gene for rickettsia, the its and pap31 genes for bartonella, and the 16s rrna gene for anaplasmataceae. positive pcr results were confirmed by using the lightcycler and specific primers for the rompb of rickettsia and glta of bartonella. r. felis was detected in 25 fleas (8.1%), w. pipientis, an insect s ...200312643829
identification of ctenocephalides felis fleas as a host of rickettsia felis, the agent of a spotted fever rickettsiosis in yucataƄ, mexico.in search for the vector of the recently recognized spotted fever rickettsiosis of the yucatán, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. the arthropods were examined by polymerase chain reaction (pcr) using primers for the genus-specific 17-kda protein gene followed by restriction fragment length polymorphism (rflp) and dna sequencing. eleven (20%) of 54 pools of ctenocephalides felis fleas contained dna of rickettsia ...200212653300
efficacy of two 65% permethrin spot-on formulations against induced infestations of ctenocephalides felis (insecta: siphonaptera) and amblyomma americanum (acari: ixodidae) on beagles.the efficacy of two formulations of a topically applied 65% permethrin spot-on (defend exspot treatment for dogs, schering-plough animal health) was evaluated against experimental infestations of the cat flea ctenocephalides felis and the lone star tick amblyomma americanum in dogs. eighteen dogs were randomly assigned to treatment with 65% permethrin in either diethylene glycol monomethyl ether (dgme; original formulation) or propylene glycol monomethyl ether (pgme) or to be untreated as a cont ...200312756635
wolbachia endosymbionts in fleas (siphonaptera).intracellular endosymbionts, wolbachia spp., have been reported in many different orders of insects and in nematodes but not previously in fleas. this is the first conclusive report of wolbachia spp. within members of the siphonaptera. using nested polymerase chain reaction (pcr) targeting of the 16s ribosomal rna gene, we screened for wolbachia spp. in fleas collected from 3 counties in georgia and 1 in new york. the prevalence of wolbachia spp. detected varied among the 6 different species scr ...200312760642
anti-complement activity in the saliva of phlebotomine sand flies and other haematophagous insects.the saliva of haematophagous insects has a series of pharmacological activities which may favour blood feeding. in the present study, an inhibitory effect on the complement system was observed in salivary extracts obtained from the phlebotomine sand flies lutzomyia longipalpis and lu. migonei. saliva from lu. longipalpis was capable of inhibiting both the classical and alternative pathways, while that from lu. migonei acted only on the former. other haematophagous insect species were screened fo ...200312885192
the feline leukemia virus (felv) and the cat flea (ctenocephalides felis). 200312928879
feline advantage heart (imidacloprid and moxidectin) topical solution as monthly treatment for prevention of heartworm infection (dirofilaria immitis) and control of fleas (ctenocephalides felis) on cats. 200312928881
severe infestation of a she-ass with the cat flea ctenocephalides felis felis (bouche, 1835).a 6-year-old she-ass was found to be severely infested with the cat flea ctenocephalides felis felis. the animal was severely anaemic and after 2 days of recumbency, it succumbed to the flea infestation. the acrimonious anaemia was characterised as normocytic, hypochromic.200312944051
efficacy of selamectin in the treatment and control of clinical signs of flea allergy dermatitis in dogs and cats experimentally infested with fleas.to determine whether treatment with selamectin would reduce clinical signs of flea allergy dermatitis (fad) in dogs and cats housed in flea-infested environments.200312959381
notes on the cat flea in puerto rico. 195313040671
techniques for rearing and handling body lice, oriental rat fleas, and cat fleas.the authors describe techniques for handling and rearing large numbers of body lice (pediculus humanus humanus l.), oriental rat fleas (xenopsylla cheopis (rothschild)), and cat fleas (ctenocephalides felis felis (bouché)).body lice may be fed on man or on domestic rabbits. in the latter case, the lice are kept on woollen patches in glass dishes at 30 degrees c and 60% relative humidity. the patches are placed on the clipped belly of a rabbit once a day and the lice allowed to feed. eggs are dep ...195413150173
allergy to flea bites. iv. in vitro collection and antigenic properties of the oral secretion of the cat flea, ctenocephalides felis felis (bouche). 196313970527
the physiological and biochemical role of the host's skin in the induction of flea-bite hypersensitivity. i. preliminary studies with guinea pig skin following exposure to bites of cat fleas. 196314053106
comparison of the activity of selamectin, fipronil, and imidacloprid against flea larvae (ctenocephalides felis felis) in vitro.the activity of selamectin, fipronil and imidacloprid against larval cat fleas (ctenocephalides felis felis) was evaluated in an in vitro potency assay system. one hundred microliters of each compound at various concentrations in acetone were added to glass vials (1.5 by 3 cm) to which had been previously added 20 mg of sand and 10 mg of flea feces. vials were then ball milled to allow the acetone to evaporate. selamectin and fipronil were tested at 0.001, 0.003, 0.005, 0.01, 0.03, 0.05, 0.11, 0 ...200314519326
evidence of horizontal transmission of feline leukemia virus by the cat flea ( ctenocephalides felis).the feline leukemia virus (felv) is a naturally occurring and widespread retrovirus among domestic cats. the virus is mainly transmitted horizontally through saliva, blood and other body fluids by close contact between cats. vectors other than cats, e.g. blood-sucking parasites, have not been reported. this study tested the vector potential of the cat flea ( ctenocephalides felis) for felv. in a first feeding, fleas were fed for 24 h with blood from a felv-infected cat with persistent viremia. f ...200314557874
cloning and characterization of five cdnas encoding peritrophin-a domains from the cat flea, ctenocephalides felis.five cdnas encoding peritrophin-a domains were identified as expressed sequence tags (ests) from flea hindgut and malpighian tubule (hmt) cdna libraries. the full-length cdnas for each were subsequently isolated and sequenced. three of the encoded proteins were similar to published peritrophin sequences, and thus were called "peritrophin-like", or pl1, pl2, and pl3. the other two sequences had similarity to both mucin and peritrophin proteins, and were called "mucin/peritrophin-like", or mpl1 an ...200314563358
efficacy and longevity of nitenpyram against adult cat fleas (siphonaptera: pulicidae).nitenpyram (capstar) is a fast acting, orally administered flea treatment that is absorbed into the blood of the host animal and is readily available for uptake by feeding fleas. we examined the efficacy of a single dose of nitenpyram against adult cat fleas, ctenocephalides felis (bouché), over several days. we recorded adult flea mortality and flea egg production on treated and untreated cats. nitenpyram provided 100% kill of all fleas on the host at the time of treatment and for up to 24 h af ...200314596282
fleas of elephant shrews (mammalia, macroscelididae) and a new host and locality record for macroscelidopsylla albertyni de meillon & marcus, 1958 (siphonaptera, chimaeropsyllidae).fleas collected from the elephant shrews elephantulus edwardii (a. smith, 1839) in the western cape province, elephantulus myurus thomas & schwann, 1906 in free state province, and an elephantulus of undetermined species in the eastern cape province, south africa are recorded in this paper. five flea species were recovered of which demeillonia granti (rothschild, 1904) was the most numerous and prevalent. it was followed by macroscelidopsylla albertyni de meillon & marcus, 1958, collected only f ...200314621322
water uptake in the cat flea ctenocephalides felis (pulicidae: siphonaptera).to counteract water loss due to excretion, cuticular transpiration and respiration, various groups of arthropods have developed mechanisms for active uptake of water vapor from unsaturated air. in this study, active uptake capabilities and water loss rates were examined in the various developmental stages of the cat flea, ctenocephalides felis. to determine critical equilibrium humidity, the lowest relative humidity at which active water uptake can occur, pre-desiccated immature and adult fleas ...200314624880
phylogenetic analysis of spotted fever group rickettsiae based on glta, 17-kda, and rompa genes amplified by nested pcr from ticks in japan.in order to understand the natural situation of rickettsiae in the ticks in japan, the rickettsial genes, glta gene, rompa gene, and 17-kda gene, were amplified from the ticks by nested pcr. the prevalences of rickettsial glta genes among haemaphysalis formosensis, h. longicornis, h. megaspinosa, ixodes ovatus, h. flava, h. kitaokai, and i. persulcatus were 62, 57, 24, 24, 19, 13, and 10%, respectively; 26% (186/722) being the average. the glta genes amplified from the ticks were classified into ...200314638993
insect allantoinase: cdna cloning, purification, and characterization of the native protein from the cat flea, ctenocephalides felis.allantoinase catalyses the hydrolysis of allantoin to allantoic acid. this reaction is a step in the purine degradation pathway, which produces nitrogenous waste for excretion. a cdna encoding full-length allantoinase was cloned from a ctenocephalides felis hindgut and malpighian tubule (hmt) cdna library. the cdna encoded a 483 amino acid protein that had 43% identity with the bullfrog rana catesbeiana allantoinase and contained the conserved histidine and aspartic acid residues required for zi ...200414871617
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