Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| immune responses of ligatured galleria mellonella larvae. | 1969 | 5777838 | |
| phagocytic haemocytes in unfixed galleria mellonella larvae. | 1969 | 5779253 | |
| virus-cell studies of tipula iridescent virus in galleria mellonella l. i. electron microscopy of infection and synthesis of tipula iridescent virus in hemocytes. | 1969 | 5784850 | |
| natural and synthetic materials with insect hormones activity. 8. juvenile activity of the farnesane-type compounds on galleria mellonella. | 1969 | 5803377 | |
| a study of the development of a nuclear-polyhedrosis virus of heliothis in galleria mellonella larvae exposed to gamma rays. | 1969 | 5804150 | |
| insect neurometamorphosis. iv. effects of the brain and synthetic alpha-ecdysone upon interganglionic connective shortening in galleria mellonella (l.) (lepidoptera). | 1969 | 5804912 | |
| changes in hemolymph total solids and blood volume of the greater wax moth, galleria mellonella, during the course of starvation. | 1969 | 5813252 | |
| resistance of galleria mellonella (linnaeus) to the tipula iridescent virus at high temperatures. | 1965 | 5827555 | |
| partial-pupae of the greater wax moth, galleria mellonella (linnaeus). | 1965 | 5848796 | |
| [histopathological study of a new type of viral disease demonstrated in the lepidoptera galleria mellonella]. | 1965 | 5858545 | |
| insect neurometamorphosis. ii. the fine structure of perineurial connective tissue, adipohemocytes, and the shortening ventral nerve cord of a moth, galleria mellonella (l.). | 1965 | 5870526 | |
| [development of the virus of the disease of dense nuclei of galleria mellonella in lepidopteran tissue culture]. | 1965 | 5870769 | |
| [the survival and differentiation in culture in vitro of gonads of final stage larvae of galleria mellonella (lepidoptera, pyralididae)]. | 1963 | 5878355 | |
| some effects of x-irradiation on larvae of galleria mellonella. | 1966 | 5939397 | |
| nucleic acid metabolism and cell interaction in the ovariole of galleria mellonella. | 1966 | 5950765 | |
| changes in some chemical constituents of galleria mellonella larvae in relation to growth and morphogenesis. | 1966 | 5962441 | |
| [in vitro cultivation of hemocytes of galleria mellonella l]. | 2004 | 5984098 | |
| neural contral of metamorphosis in galleria mellonella (lepidoptera). | 1966 | 6004953 | |
| sensory innervation of the spiracular muscle in the tsetse fly (glossina morsitans) and the larva of the waxmoth (galleria mellonella). | 1966 | 6004954 | |
| incorporation of 14-c-sodium palmitate into lipids and cell interaction in ovarioles of galleria mellonella (lepidoptera). | 2005 | 6010829 | |
| insect neurometamorphosis. 3. nerve cord shortening in a moth, galleria mellonella (l.) may be accomplished by humoral potentiation of neuroglial motility. | 1967 | 6040557 | |
| the virulence of protease and cell surface mutants of pseudomonas aeruginosa for the larvae of galleria mellonella. | 1982 | 6123536 | |
| influence of biologically active substances isolated from galleria mellonella on neurons of lymnaea stagnalis in culture. | a procedure for isolating biologically active substances from galleria mellonella using a culture of isolated giant neurons of mollusc lymnaea stagnalis as a test-system is described. fractions capable of activating neurites and inhibiting aggregation of neuronal cells within a range of concentrations from 1 to 30 micrograms/ml were isolated. the fractions obtained have in their chemical composition about 10.5% n, also contain p and s. they have a carbohydrate component. | 1984 | 6146471 |
| stimulation of rna transcription by juvenile hormone in degenerating silk glands. | rna transcription in the silk glands of the wax moth, galleria mellonella, decreases during cocoon spinning and particularly later in pharate pupae, when the glands begin to degenerate. application of juvenile hormone to insects at those developmental stages enhances synthesis of polyadenylated rna 4-6 times and to lesser extent synthesis of total rna. the newly formed poly(a)rna appears identical with that from the normally fully functioning and silk-producing glands: it contains the same predo ... | 1982 | 6176479 |
| observations on the cytochemistry of the hemocytes of an insect, galleria mellonella. | a number of cytochemical parameters of the hemocytes of larval galleria mellonella, an insect frequently used as a model by comparative cellular immunologists, are described. cytochemical methods were used to quantify hemocyte granule-associated components, the results are compared to those obtained for leukocytes from higher animals. granulocytes contained a population of nonlysosomal granules rich in mucopolysaccharide not seen in plasmatocytes. the numbers and dimensions of these granules sho ... | 1983 | 6188780 |
| photoreactivation and ultraviolet-enhanced reactivation of ultraviolet-irradiated nuclear polyhedrosis virus by insect cells. | the nuclear polyhedrosis virus (baculovirus) of galleria mellonella (pryalidae: lepidoptera) was used to investigate the capability of cultured insect cells to repair ultraviolet (uv) induced damage in the viral genome. when assayed by the formation of plaques in the cell line tn-368, the survival of the virus was found to decrease linearly with increased ultraviolet exposure. the infectious capacity of uv-irradiated virions was significantly restored after exposing the tn-368 monolayers to eith ... | 1984 | 6365037 |
| nucleotide sequence of the galleria mellonella nuclear polyhedrosis virus origin of dna replication. | the initiation sites of the galleria mellonella l. nuclear polyhedrosis virus (g.m. npv) dna replication were revealed. for this purpose scld 135 cells permitting the g.m. npv productive reproduction were transformed by the recombinant plasmids containing the viral genome individual fragments in prsf 2124 and pbr 322 vectors. it was revealed that 2 of the 32 recombinant plasmids can autonomously replicate in the eucaryotic cells. according to the maxam-gilbert method the dna g.m. npv fragment (1 ... | 1984 | 6365591 |
| structural alterations to proteus mirabilis as a result of exposure to haemolymph from the larvae of galleria mellonella. | scanning and transmission electron microscopy was used to examine proteus mirabilis, strain 11-1a, for morphologic alterations following exposure to normal and immune cell-free haemolymph obtained from galleria mellonella larvae. it was found that both normal and immune haemolymph exerted effects on the bacterial cells, with immune haemolymph affecting a much higher percentage of cells than normal haemolymph. the morphological damage observed by scanning electron microscopy (sem) included abnorm ... | 1984 | 6374385 |
| transfer of immunity against pseudomonas aeruginosa p11-1 in galleria mellonella larvae. | larvae of galleria mellonella may be immunized against pseudomonas aeruginosa by the transfer of whole hemolymph, cell-free hemolymph or hemocytes from insects previously immunized with the lipopolysaccharide of the homologous organisms. whole immune hemolymph injected as early as 3 hours after vaccination confers protection which persists as long as 40 hours. hemocytes alone confer good protection when transferred as early as 30 minutes after, and up to about 4 hours after immunization. transfe ... | 1983 | 6416902 |
| the in vitro generation of an antibacterial activity from the fat body and hemolymph of non-immunized larvae of galleria mellonella. | a state of immunity in galleria mellonella against the pathogen pseudomonas aeruginosa is known to be induced by the injection of lipopolysaccharide (lps), isolated from the homologous organism. an in vitro mixture of the lps and whole or cell-free hemolymph from non-immunized larvae is not antibacterial. in vitro mixtures of fat body and cell-free hemolymph from non-immunized larvae, incubated at 25 degrees c for 20 hours generated a proteinaceous antibacterial activity. the generation of this ... | 1984 | 6437887 |
| changes in ecdysteroid and juvenile hormone titers in the hemolymph of galleria mellonella larvae and pupae. | the variations in circulating ecdysteroids and juvenile hormones (jh) in galleria, from the end of the antepenultimate larval stage until emergence of adults, have been determined. the two hormonal families were extracted separately from the same hemolymph sample and quantified by two radioimmunoassays. juvenile hormone ria activity was about 35 nm in larvae of the antepenultimate and penultimate stages. it dropped before each molt and increased thereafter. moreover, it gradually decreased durin ... | 1984 | 6510685 |
| [the effects of bacillus thuringiensis on the greater wax moth, galleria mellonella (l.) (lepidoptera: galleriidae)]. | the effects of bacillus thuringiensis preparation on the greater wax moth population have been investigated under laboratory conditions. b. thuringiensis isolated from commercial insecticidal formulations such as dipelr (abbott lab. usa), tribactur btb wp (fabrick von chemische production, holland), bactospein (laboratoire roger, france). the effective strain was used in this experiment. the applied concentrations of bacillus on the greater wax moth larvae are 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 gram, ... | 1983 | 6669083 |
| the synthesis of rna and silk protein in galleria mellonella silk glands. | the silk protein synthesis in silk glands of galleria mellonella is preceded by the increase of total rna content. the levels of the main rna classes: 28s and 18s rrna, trna as well as poly(a) + rna change proportionally to the total rna pool of glands. the fibroin-like silk protein of molecular weight of about 240 000 is characterized by the high content of four amino acids: glycine, alanine, serine and leucine, which account for more than 70% of amino acid residues. this fibroin-like protein i ... | 1984 | 6720190 |
| calcium binding sites in the insect polytrophic egg vesicles as possible markers of the route of inflow of calcium. | calcium binding sites were revealed in the polytropic egg vesicles of galleria mellonella (lepidoptera) by fixation of the ovariole with glutaraldehyde in the presence of excess of calcium. calcium dependent deposits appeared in the mitochondrial matrix in cells of the epithelial sheath and of the follicular epithelium. the deposits were also seen in the extracellular space around the follicular epithelial cells and around the trophocytes , at the oolemma, and in the coated vesicles of the oocyt ... | 1984 | 6735280 |
| the inhibitory effect of purified cobra venom factor, isolated from the venom of naja naja atra, on the in vivo immune response in galleria mellonella. | 1981 | 6786928 | |
| effects of hemolymph from immune and non-immune larvae of galleria mellonella on the ultra-structure of pseudomonas aeruginosa. | the ultrastructure of pseudomonas aeruginosa, a pathogen of galleria mellonella is rapidly altered after in vitro exposure to the hemolymph of vaccinated larvae. the bacteria were treated with normal and immune hemolymph for periods of time ranging from 7 to 28 min at 28 degrees c. in contrast to the apparent non-damaging effects of normal hemolymph, the immune hemolymph caused progressive damage to the cells within 7 min. the initial attack was directed towards the cell wall. complete degradati ... | 1982 | 6813153 |
| [a method for the ultrastructural, immunocytochemical detection of ecdysone]. | the investigations were carried out with prothoracic glands of galleria mellonella. the influence of various fixation media on the preservation of the ultrastructure and on the immunocytochemical staining of the steroid ecdysone was studied. an immunocytochemical staining technique for ecdysone is described allowing the ultrastructural localization of ecdysone in different cellular compartments. | 1982 | 6815959 |
| heterorhabditis spp., neoaplectana spp., and steinernema kraussei: interspecific and intraspecific differences in infectivity for insects. | the effectiveness of various dosages of different species/strains of nematodes was compared for galleria mellonella and various pest insects that live in or pupate in soil. neoaplectana feltiae (= carpocapsae), the only nematode species tested by most other workers, was never the most infective for any of the insect species tested and was least infective for two. all species/strains of nematode were able to kill insects of each species. the degree of infectivity of each of the nematode species/s ... | 1983 | 6832283 |
| structural and thermodynamic investigation of the chilo iridescent virus (iridovirus type 6). | structural features and thermodynamic parameters of the complete chilo iridescent virus (iridovirus type 6) and its constituents, isolated from the larvae of galleria mellonella, were evaluated by means of uv spectroscopy and microcalorimetry. it can be demonstrated that the viral dna is attached to the coat protein in a chromatin-like fashion, which is preserved after disruption of the virus by low temperature or partial digestion of the coat protein with proteinase k. at elevated temperature h ... | 1983 | 6838381 |
| ultrastructural and immunocytochemical investigation of ecdysteroid secretion by the prothoracic gland of the waxmoth galleria mellonella. | the formation and secretion of ecdysteroid by the prothoracic gland cells of galleria mellonella (insecta, lepidoptera) were investigated electron microscopically and immunocytochemically. the moulting hormone ecdysone becomes first evident in vesicles and tubules of the smooth endoplasmic reticulum (ser). the ser forms secretory granules in which ecdysone was shown immunocytochemically. the golgi apparatus seems not to be directly involved in ecdysone secretion. the secretory granules are relea ... | 1983 | 6850754 |
| further studies on the effect and role of cobra venom factor on protective immunity in galleria mellonella: activity in the response against proteus mirabilis. | 1980 | 6995190 | |
| [derivation of a transplantable cell line from oak silkworm ovarian tissue]. | a continuous cell line (m. s. a. p.-1) was obtained from the ovaries of diapausing pupae of the oak silkmoth antheraea pernyi. active multiplication of cells was observed two months after preparing a primary culture from partly trypsinized tissues. subcultures derived at this time were transmitted with 10 day intervals and endure over 30 passages. the cells are susceptible to the nuclear polyhedrosis viruses of mamestra brassicae, galleria mellonella, malacosoma neustria and bombyx mori. | 2008 | 6998064 |
| [quantitative determination of the infectivity of nuclear polyhedrosis virus dna on honeycomb moth (galleria mellonella) larvae]. | the possibility of using honeycomb moth larvae for titration of nuclear polyhedrosis virus (npv) infectious dna and determinations of transfection effectiveness was studied. honeycomb moth larvae were shown to be a sensitive system for npv dna titration. deae-dextran used as a protector increased npv dna infectivity 1000-fold, ld50 in this instance being 2 x 10(8) molecules per larva. the method of npv dna infectivity determinations by the number of larvae with polyhedreae in the fatty tissue is ... | 1980 | 7001752 |
| [a non-damaging procedure for dna isolation from insect cell nuclei]. | a gentle procedure is described for dna isolation from galleria mellonella cell nuclei. nuclei lysis was performed by sds in final concentration of 3% which was followed by dna extraction from chromatin by a stepwise rise of nacl concentration up to 2.5 m. after removal of nuclei insoluble components by centrifugation the native dna was purified using the hydroxyapatite column. the method proposed provides a higher yield of the native dna as compared with the phenol-detergent method. | 2015 | 7101477 |
| juvenoids cause some insects to form composite cuticles. | metamorphosing insects treated with juvenoids may secrete composite cuticles which combine morphological features of two metamorphic stages within the area secreted by an individual epidermal cell. characters found combined were pigmentation, tanning, surface sculpturing, and microtrichiae. neighbouring cells frequently form different types of cuticle. composite cuticles should not be confused with the more common mosaic cuticles, which are composed of discrete areas with different stage-specifi ... | 1982 | 7153697 |
| [dynamics of egg maturation in the parasitoid lixophaga diatraeae (diptera, tachinidae) raised in a substitute host, galleria mellonella (lepidoptera, pyralidae)]. | the content of the uterus and ovaries of l. diatraeae (an ovolarviparous tachinid fly) was studied by dissecting 121 females 0 to 25 days old. several size classes of flies (p: small; m: medium; g: big) were used, weighing 10 to 12.5 mg, 12.5 to 13.5 mg, and 13.5 to 16 mg, respectively. the mean number of ovarioles per ovary, correlated to female size, was 12.1 for p as against 12.9 for m + g females. the total mean number of eggs and oocytes changed with female age and weight. for 0, 5, 10 and ... | 1982 | 7156496 |
| calcium content and distribution in egg vesicles of galleria mellonella (lepidoptera) as determined by x-ray microanalysis. | in egg vesicles of galleria mellonella (lepidoptera) electron microprobe analysis reveals calcium in concentrations of 9 and 3 mmoles per 1,000 g tissue wet weight in oocyters and accompanying trophic cells, respectively. this high average level of calcium characterizes both pre- and postvitellogenic oocytes, but the distribution of calcium is not uniform. in postvitellogenic vesicles the central area of the ooplasm shows a higher content of ca than peripheral one, what may be correlated with th ... | 1980 | 7190558 |
| new insecticidal cyclodepsipeptides from the fungus isaria felina. i. production, isolation and insecticidal properties of isariins b, c and d. | three new cyclodepsipeptides related to the previously described isariin were isolated from a strain of isaria felina. they were named isariins b, c and d. isariin d and, to a lesser extent, isariin c, exhibited insecticidal activity against galleria mellonella larvae, whereas isariin b and isariin itself proved inactive. | 1981 | 7198111 |
| tests with inactivated cricket paralysis virus as a possible immunogen against a virus infection of galleria mellonella larvae. | the antibody-binding capacity of cricket paralysis virus was only slightly reduced by inactivation with heat or formaldehyde. however, galleria mellonella larvae vaccinated with such inactivated cricket paralysis virus preparations and subsequently challenged with live homologous virus were not protected. | 1980 | 7203971 |
| effect of disulfiram on the ultrastructure of the peptidergic and aminergic cells in the pars intercerebralis of galleria mellonella (lepidoptera). | injection of disulfiram, a specific inhibitor of noradrenaline synthesis, evokes changes in two different types of neurons in the brain of galleria mellonella: neurones containing dense-core vesicles (60-80 nm) and peptidergic cells containing 180-200 nm neurosecretory granules. the lowering of the electron density of the dense-core vesicles of 60-80 nm seems to be directly connected with disulfiram administration, while the decrease of the amount of neurosecretory material in the neurosecretory ... | 1981 | 7214484 |
| ultrastructural study of the postembryonic development of the neural lamella of galleria mellonella l. (lepidoptera). | the neural lamella encapsulating the brain of the wax moth galleria mellonella develops from a very thin (80-120 nm) layer in the first larval instar, resembling the basal lamina, to a thick (1-4 micrometer) sheath composed of two zones in the seventh (last) instar. after its breakdown at the time of larval-pupal ecdysis the neural lamella is reconstructed in the pupa, 2-3 days before pupal-adult ecdysis. the cells of the perineurium seem to be responsible for the formation of the neural lamella ... | 1981 | 7237538 |
| insect immunity. purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of hyalophora cecropia. | three inducible bacteriolytic proteins, designated p7, p9a and p9b, from the hemolymph of immunized pupae of the giant silk moth hyalophora cecropia have been purified using a two-step procedure with cation-exchange chromatography. purified protein p7 has a molecular weight of 15000 and its amino acid composition shows a great similarity to that of the lysozyme from the wax moth galleria mellonella. moreover, heat stability, ph-rate profile and bacteriolytic specificity also indicate that protei ... | 1980 | 7341234 |
| segmental gradients specifying polarity and pattern in the wax moth, galleria mellonella: the posterior margin as the source of a diffusible morphogen. | 1980 | 7350014 | |
| graded inhibition of cell disintegration of juvenile hormone. | the proventriculus of galleria mellonella larvae consists of 260-300 polyploid cells. the cells grow throughout larval development and at each ecdysis produce a new cuticular lining of the proventriculus. each cell secretes a large sclerotized plaque covered with thorns. at the end of the last larval instar, the cells disintegrate unless the larvae is treated with juvenile hormone or implanted with active corpora allata. depending on the time of treatment and the hormone dose, these insects pres ... | 1980 | 7379132 |
| ultrastructural study on the innervation of prothoracic glands in galleria mellonella. | 1980 | 7409448 | |
| density-dependent effects on steinernema glaseri (nematoda: steinernematidae) within an insect host. | increasing densities of steinernema glaseri infective juveniles (ijs) in soil affected penetration efficiency and reproduction of the nematodes in larvae of the greater wax moth galleria mellonella. the penetration efficiency and the proportion of penetrated ijs developing to adults decreased significantly with increasing numbers of ijs present in the soil and entering the hosts, respectively. the number of progeny produced/host cadaver initially increased, with the highest production being betw ... | 1995 | 7472882 |
| fatty acid profiles of invertebrate iridescent viruses. | eight invertebrate iridescent viruses (ivs) from diverse host taxa were grown in a common lepidopteran host, galleria mellonella. the lipid composition of purified virus was assessed by fatty acid methyl esterase (fame) analysis using a gas-liquid chromatograph. iv fatty acid profiles were markedly different from those of the host tissues. the interrelationships among the ivs did not follow previous serological and genetic findings. we conclude that fame analysis is not a useful technique for re ... | 1995 | 7605209 |
| separation of oviposition-stimulating peptides and myotropic factors from head extracts of galleria mellonella l.: comparative effects of myotropic and non-myotropic factors on egg laying. | methanolic extracts from heads of the wax moth, galleria mellonella l. contain several factors that stimulate oviposition of virgin females in vivo and spontaneous contractions of the oviduct in vitro of the cricket, gryllus bimaculatus. the myotropic and egg-laying activities behaved distinctly during all steps of purification, including fractionation on reverse-phase high-performance liquid chromatography. a novel neuropeptide was isolated from galleria mellonella. the peptide does not increas ... | 1995 | 7622672 |
| analysis of ecdysteroid action in malacosoma disstria cells: cloning selected regions of e75- and mhr3-like genes. | ipri-md-66 (md-66) cells respond to 20-hydroxyecdysone (20e, 4 x 10(-6) m) in the medium by producing cytoplasmic extensions, clumping and attaching themselves to the substrate. these morphological changes are at a maximum by 6 days post treatment. degenerate oligonucleotides, designed on the basis of conserved amino acid sequences in the dna and ligand binding regions of the members of the steroid hormone receptor superfamily, were used in rna-pcr to isolate two cdna fragments, malacosoma disst ... | 1995 | 7627201 |
| multicellular-vesicle-promoting polypeptide from trichoplusia ni: tissue distribution and n-terminal sequence. | an n-terminal amino acid sequence of a 16.9 kda hemolymph polypeptide, "vesicle promoting factor" (vpf) from trichoplusia ni, revealed a high sequence homology (70%) with manduca sexta apolipophorin-iii. a polyclonal antibody developed against vpf, however, was not immunoreactive with either purified m. sexta or t. ni apolipophorin-iii. immunoblots of tissue homogenates of t. ni indicated that vpf was present in imaginal wing discs, central nervous system (cns), silk glands, midgut and hemocytes ... | 1995 | 7655058 |
| hirsutellin a, a toxic protein produced in vitro by hirsutella thompsonii. | a toxic protein, hirsutellin a, has been purified from the mite fungal pathogen, hirsutella thompsonii, using ammonium sulphate precipitation, ion exchange chromatography and gel filtration on bio-gel p-10. the protein has been characterized as a monomer with a molecular mass of 15 kda and an isoelectric point of 10.5. the amino acid composition and the n-terminal sequence of hirsutellin a (34 amino acids) have been determined. from these results, the toxin appears to be distinct from other know ... | 1995 | 7670635 |
| light-chain fibroin of galleria mellonella l. | the posterior section of galleria mellonella silk glands contains two abundant mrnas that are identical except for the non-coding tail, which includes either two (1.1 kb mrna) or three (1.2 kb mrna) consensus sequences for polyadenylation sites. the transcripts are 40% homologous in the coding as well as non-coding regions with the mrna encoding light-chain fibroin (l-fibroin) in bombyx mori; the deduced translation product shows 43% identity with the bombyx l-fibroin peptide, with all three cys ... | 1995 | 7715595 |
| expression cloning and characterization of a pupal cuticle protein cdna of galleria mellonella l. | epidermal mrna of freshly ecdysed pupae of galleria mellonella was used to establish a cdna library in phage lambda gt11. a cdna clone was isolated by means of a pupal cuticle protein (pcp) specific antibody. nucleic acid sequence data show an insert of 1212 bp with an open reading frame of 1062 bp. the presence of start, stop, and polyadenylation sites suggests, that this insert represents a full length transcript. conceptual translation resulted in a protein of 353 amino acids including a sign ... | 1995 | 7773255 |
| plaque assay and replication of tipula iridescent virus in spodoptera frugiperda ovarian cells. | a plaque assay was developed for the study of tipula iridescent virus (tiv) replication using a cell line derived from the fall army worm spodoptera frugiperda (sf9). infection and plaque formation were monitored with time by phase contrast microscopy, video and fluorescent light microscopy. structure of virions, viroplasmic centres and organelles of infected cells were examined by transmission electron microscopy (tem). after 4 h postinfection, plaques were visibly detected within the cell mono ... | 1994 | 7839010 |
| isolation and characterization of a hemocyte aggregation inhibitor from hemolymph of manduca sexta larvae. | a protein that inhibits hemocyte aggregation has been isolated from hemolymph of manduca sexta larvae and named hemocyte aggregation inhibitor protein (haip). haip has a m(r) = 50,000, pi = 8.5, and contains 7% carbohydrate. it is present at 230 +/- 20 micrograms/ml in hemolymph of day 3 fifth instar larvae. antibodies to haip do not cross-react with m. sexta hemolin, which is similar in size and charge and also inhibits hemocyte aggregation. haip and hemolin have some similarity in amino acid c ... | 1994 | 7949257 |
| isolation and developmental expression of the ecdysteroid-induced ghr3 gene of the wax moth galleria mellonella. | three degenerate primers were designed to match the most conserved regions within the dna-binding domains of several selected members of the steroid hormone receptor family. use of these primers in the polymerase chain reaction with cdna from galleria mellonella prepupae detected a 177 bp fragment that had 87% identity to the manduca sexta gene mhr3 and 75% to the drosophila melanogaster dhr3 gene, and therefore was named "ghr3". screening of a galleria penultimate instar cdna library with this ... | 1994 | 7981726 |
| interaction of mutants of xenorhabdus nematophilus (enterobacteriaceae) with antibacterial systems of galleria mellonella larvae (insecta: pyralidae). | xenorhabdus nematophilus mutants that took longer to kill insects than did the wild type were used to determine the relationship of the physicochemical properties and outer membrane composition to bacterial interaction with the antibacterial systems of galleria mellonella larvae and to bacterial virulence. insect serum slowed the growth of the wild-type and mutant bacteria. this was attributed to increased spheroplast formation for the mutants. spheroplast formation was associated with an increa ... | 1994 | 8012904 |
| adaptogenic and cardioprotective action of galleria mellonella extract in rats and frogs. | pharmacological and metabolic effects of galleria mellonella larvae extract used in russian folk medicine to treat cardiovascular and senile diseases were studied. it was shown that the extract possesses adaptogenic, cardiotropic, cardioprotective, and hypocoagulant properties. the extract possesses low toxicity and does not cause significant changes in biochemical parameters in the blood serum of laboratory animals. increase in catecholamine content in the heart and aortic tissues and their dec ... | 1994 | 8027932 |
| gallysin-1, an antibacterial protein isolated from hemolymph of galleria mellonella. | an inducible hemolysin with antibacterial properties was isolated from the hemolymph of immune galleria mellonella larvae. the galleria-derived lysin, named gallysin-1, was shown to have an apparent molecular weight of 75,000 and to be relatively heat stable at 56 degrees c. although gallysin alone was not bactericidal it caused sufficient damage of the outer cell membranes of pseudomonas aeruginosa rp4 and escherichia coli k176 to release beta-lactamase from the periplasm. in the presence of ei ... | 1994 | 8050612 |
| nucleotide sequence, structure and developmental regulation of lhp82, a juvenile hormone-suppressible hexamerin gene from the waxmoth, galleria mellonella. | we cloned and sequenced a composite cdna corresponding to the 2.6 kb last instar-specific, juvenile hormone-suppressible lhp82 mrna from galleria mellonella. the identity of the cdna was confirmed by n-terminal amino acid sequencing of the purified lhp82 subunit. in addition, we sequenced all coding regions and most of the intronic dna as well as 1300 nucleotides of 5' flanking dna from the lhp82 gene. the eight exons of the lhp82 gene specify a pre-protein of 706 residues, including the signal ... | 1994 | 8111425 |
| asthma caused by live fish bait. | larvae of insects and worms are commonly used as live fish bait (lfb) by anglers. asthma, rhinoconjunctivitis, and urticaria related to various kinds of lfb have been reported. | 1994 | 8120269 |
| isolation, characterization and developmental expression of the ecdysteroid-induced e75 gene of the wax moth galleria mellonella. | using the cdna for the drosophila ecdysteroid-induced member of the steroid-hormone-receptor superfamily, e75a, we isolated a genomic clone from galleria mellonella that revealed 77% similarity with the region of e75a cdna encoding the c-terminal zinc-finger motif. a galleria cdna clone was isolated that encoded a complete dna-binding domain composed of two zinc fingers and designated gme75a. its deduced amino acid sequence showed 100% and 85% identities within the dna-binding and ligand-binding ... | 1994 | 8174547 |
| induction kinetics of immune antibacterial proteins in pupae of galleria mellonella and pieris brassicae. | 1. pupae of galleria mellonella and pieris brassicae given an injection with live, non-pathogenic enterobacter cloacae or abiotic foreign molecules induce an acquired immunity that corresponds with the synthesis of haemolymph proteins of antibacterial activity. 2. this humoral defensive response which persists for several days, differs quantitatively between insect species and between the inducers used, although very different foreign bodies induced the same immune proteins in both lepidopteran ... | 1993 | 8243062 |
| purification and characterization of a flavin-binding storage protein from the hemolymph of galleria mellonella. | the 85k storage protein that accumulates in the hemolymph of galleria mellonella during the final larval instar was isolated and purified from newly molted pupae. the separation of fresh hemolymph proteins from larvae or pupae by different chromatographic and electrophoretic procedures indicated the native protein had a m(r) of 170,000 and consisted of two identical 85k subunits. crosslinking experiments using fresh hemolymph followed by western blotting also indicated a dimeric structure for th ... | 1994 | 8292819 |
| further observations on the induction of immunity by hemolymph transfer in galleria mellonella. | humoral immune responses of galleria mellonella larvae were provoked by transfer of hemolymph lysate supernatant preparations (hls) from untreated donor larvae to recipient larvae. eighteen hours after transfer the antibacterial activity in cell-free hemolymph of recipient larvae was as high as after injection of high dosages of living enterobacter cloacae beta 12 bacteria. filtration of hls (fhls; 0.2 microns filter) prior to injection enhanced its induction capacity. injection of fhls concentr ... | 1993 | 8375564 |
| toxicity and pathology of crude broth filtrate produced by hirsutella thompsonii var. thompsonii in shake culture. | metabolic products secreted by the fungal mycelia of hirsutella thompsonii var. thompsonii (cbs 556.77d) in a defined culture broth in shake culture were tested for toxicity to galleria mellonella larvae and drosophila melanogaster adults via injection and per os application, respectively. in addition, the toxic effect of broth filtrate was observed in vitro in a cell line of bombyx mori. czapek-dox broth fortified with 1% yeast extract stimulated more rapid mycelial growth and correspondingly m ... | 1993 | 8463709 |
| extraction and characterization of the insecticidal toxin hirsutellin a produced by hirsutella thompsonii var. thompsonii. | hirsutellin a (hta) produced by hirsutella thompsonii var. thompsonii (strain jab-04) was extracted and purified using a combination of ion-exchange, gel-permeation, and immunoaffinity chromatography. the identity of the purified hta was confirmed by amino acid analysis and n-terminal sequencing. monoclonal antibodies prepared against hta were capable of detecting 25-50 ng of hta by direct sandwich elisa. in addition, utilizing western blot methods, the antibodies were shown to be specific to ht ... | 1995 | 8574903 |
| the prophenoloxidase from the wax moth galleria mellonella: purification and characterization of the proenzyme. | a prophenoloxidase (ppo) was purified from the hemolymph of the larvae of galleria mellonella. a 135-fold purification of the proenzyme with 25% yield was achieved by a combination of different chromatographic methods. an alternative micropreparation of pure ppo by a novel method for native electrophoresis in polyacrylamide gel is also described. the molecular mass of the native ppo was estimated to be 300 kda by the pore-limit gradient electrophoresis in polyacrylamide gel. in the presence of s ... | 1995 | 8580908 |
| the effect of temperature on infection, development and reproduction of heterorhabditids. | the effect of temperature on infection, development and reproduction of five heterorhabditis isolates was examined in galleria mellonella larvae. infectivity was examined at six temperatures (5, 10, 15, 20, 25 and 30 degrees c). the proportion of galleria infected remained relatively constant, only dropping at extremes of the temperature range tested. however, the mean number of nematodes per host produced a different trend, with each isolate displaying optimal infectivity at 25 degrees c. the t ... | 1995 | 8583129 |
| purification and characterization of lysozyme from hemolymph of heliothis virescens larvae. | lysozyme is an important antibacterial protein in the insect defense system. lysozyme was isolated from hemolymph of heliothis virescens larvae using gel filtration and ion-exchange chromatography. heliothis lysozyme had a molecular mass of 16,000 daltons by sds-page. using acid gel electrophoresis, heliothis lysozyme migrated faster than egg white lysozyme. the pi of heliothis lysozyme was estimated as greater than 9.5. heliothis lysozyme had specific bactericidal activity against three gram-po ... | 1996 | 8607795 |
| ecology of anti-microbials produced by bacterial associates of steinernema carpocapsae and heterorhabditis bacteriophora. | based on the ability of bacterial associates of entomopathogenic nematodes to produce antibiotic compounds on artificial media, it has been commonly accepted that xenorhabdus sp. and photorhabdus sp. inhibit a wide range of invading microorganisms in insects infected with steinernema spp. or heterorhabditis spp. therefore, the question of whether antibiotic compounds produced by the primary form of bacterial symbionts associated mutualistically with s. carpocapsae and h. bacteriophora explain wh ... | 1996 | 8684829 |
| allatotropin released by the brain controls larval molting in galleria mellonella by affecting juvenile hormone synthesis. | monoclonal antibodies were raised against a protein fraction which was purified from larval brains of galleria mellonella and which was shown to stimulate the corpora allata to synthesize juvenile hormone in vivo as well as in vitro. immunoblot analysis revealed that one polypeptide band of 20 kda was specifically recognized. two pairs of median neurosecretory cells of the brain and the cells of the corpus cardiacum were demonstrated to be immunoreactive to the antibodies. our results sustain th ... | 1996 | 8735930 |
| rapid changes in thermal sensitivity of entomopathogenic nematodes in response to selection at temperature extremes | entomopathogenic nematodes (rhabditida: heterorhabditidae, steinernematidae) are lethal insect parasites that have a symbiotic association with bacteria in the family enterobacteriaceae. we evaluated the changes in thermal sensitivity of two nematodes heterorhabditis bacteriophora and steinernema anomali. the nematodes were genetically selected, together with their respective symbiotic bacteria, at nematodes' reproductive thermal niche breadth extremes, 15° and 30°c, by repeated passage ... | 1996 | 8812571 |
| peptide rgds inhibits the fibronectin-enhanced phagocytosis of yeast cells by galleria mellonella hemocytes in vitro. | 1996 | 8858912 | |
| host recognition behaviour predicts host suitability in the entomopathogenic nematode steinernema carpocapsae (rhabditida:steinernematidae). | steinernema carpocapsae (rhabditida: steinernematidae) host recognition behaviour was assessed and compared with 2 measures of host suitability. previous research showed that s. carpocapsae infective juveniles respond to host cues in a hierarchical order, with attraction to galleria mellonella volatiles being stimulated by contact with g. mellonella cuticle. we measured host recognition behaviour by calculating the percentage response of s. carpocapsae infective juveniles to volatiles produced b ... | 1996 | 8939054 |
| isolation, characterization and complete nucleotide sequence of a galleria mellonella cdna coding for the follicle cell-specific yolk protein, yp4. | an ovariole-specific yolk polypeptide cdna from galleria mellonella was isolated from a cdna library constructed using vitellogenic ovariole poly (a)+ rna, by differential screening with the ovariole and day-0 male pupal poly (a)+ rna. the ov11 cdna hybridized to a 1.1 kb message from the ovariole but not from male pharate adults, ovariectomized pharate adults or day-5 last instar larvae. developmental northern analysis showed that this message is expressed only during vitellogenic stages. in si ... | 1996 | 8969466 |
| evidence for glycosylation of the juvenile-hormone-binding protein from galleria mellonella hemolymph. | the juvenile-hormone-binding protein (jhbp) from galleria mellonella hemolymph, which is a member of the high-affinity/low-molecular-mass group of jhbp proteins, was found to be glycosylated. glycosylation was confirmed by the following evidence. carbohydrate gas-liquid chromatography analysis of the purified jhbp preparations showed the presence of a low amount of sugars (man and glcnac were the major components). the jhbp electrophoretic band blotted onto nitrocellulose was stained with glycot ... | 1996 | 9022705 |
| a new small rna virus persistently infecting an established cell line of galleria mellonella, induced by a heterologous infection. | a persistent infection in a galleria mellonella cell line was revealed when infected with a maize stem borer picorna-like virus isolated on sesamia cretica (msbv). the new virus, completely different from the msbv, is designated as g. mellonella cell line virus (gmclv), induces spectacular cytopathic effects, and is also considered efficient in vivo. the gmclv is a 29-nm-diameter isometric virus, with single-strand rna of 2.9 x 10(6) da molecular weight with a poly(a) tract. its capsid is consti ... | 1997 | 9028922 |
| immunoaffinity purification of juvenile hormone-binding protein from galleria mellonella hemolymph. | previously described methods of purification of hemolymph juvenile hormone-binding protein (hjhbp) from lepidoptera were tedious and required multiple steps. these methods resulted in low protein yield (kramer et al., 1976; goodman et al., 1978; peterson et al., 1982; park et al., 1993; ozyhar & kochman, 1987). in this report a simple method of purification of hjhbp from galleria mellonella (l.) larvae is described. monoclonal antibodies against hjhbp were obtained and crosslinked to cnbr-activa ... | 1996 | 9104496 |
| influence of low temperature on the synthesis of some galleria mellonella proteins. | in developing galleria mellonella larvae (reared at 30 degrees c) three proteins of 74, 76 and 81/82 kda were identified. they represent a group of storage proteins (lhp proteins). in galleria larvae, the development of which is arrested by low temperature (18 degrees c), accumulation of the 74, 76 and 81/82 kda proteins was detected in the hemolymph. the synthesis of 74 kda and 76 kda proteins started after 24 h, and that of about 80 kda after 96 h following the transfer of larvae from 30 degre ... | 1996 | 9104500 |
| mechanisms of specificity of association between the nematode steinernema scapterisci and its symbiotic bacterium. | we suggest a new mechanism for the maintenance of specificity of the association between the entomopathogenic nematode steinernema scapterisci and its symbiotic bacteria. we evaluated the development and reproduction of infective and non-infective juvenile s. scapterisci in monoxenic combinations with its symbiotic bacteria, xenorhabdus sp. 's' and with the bacterial symbiont of steinernema carpocapsae and steinernema riobravis. although development of non-infective stages occurred on all xenorh ... | 1997 | 9149419 |
| pathogenicity of axenic steinernema feltiae, xenorhabdus bovienii, and the bacto-helminthic complex to larvae of tipula oleracea (diptera) and galleria mellonella (lepidoptera). | the pathogenicity of the nematode-bacterium complex steinernema feltiae-xenorhabdus bovienii to larvae of tipula oleracea and galleria mellonella was investigated by injection of dauer juvenile nematodes carrying their bacterial symbiont cells (monoxenic nematodes). axenic nematodes (free of bacteria) and the symbiotic bacteria themselves were tested. the lc50 of x. bovienii in t. oleracea was 15,700 colony forming units (cfu)/larva compared to < or = 8 cfu in g. mellonella. xenorhabdus bovienii ... | 1997 | 9170346 |
| effects of infected insects on secondary invasion of steinernematid entomopathogenic nematodes. | factors affecting 'invasion efficiency' of steinernematid entomopathogenic nematodes into hosts were elucidated. the phenomenon that only part (10-40%) of the nematode population invades the target host has been recorded in many studies. it has been mainly ascribed to differences in the ability of individual nematodes to infect. in the present study the effect of an infected host, the wax moth galleria mellonella, on subsequent infection of the entomopathogenic nematodes steinernema carpocapsae ... | 1997 | 9172429 |
| assay for phenoloxidase activity in amblyomma americanum, dermacentor variabilis, and ixodes scapularis. | phenoloxidase activity in amblyomma americanum, dermacentor variabilis, and ixodes scapularis was assayed. no phenoloxidase activity was detected compared to high activity through time in larvae of the greater wax moth (galleria mellonella). we conclude that activated prophenoloxidase does not act as an opsonin in ixodid ticks tested to date. | 1997 | 9194851 |
| mechanical work as a determinant of prey-handling behavior in the tokay gecko (gekko gecko). | in this study an in vitro analysis of the force and mechanical work required to bite prey items of different size and physical character is combined with an in vivo analysis of prey-handling behavior in the tokay gecko (gekko gecko). the force required to bite and the work of biting increase with prey size, but the rate of increase is prey specific, with crickets (acheta domestica) requiring substantially more force and work per bite than larvae (galleria mellonella and manduca sexta) for all bu ... | 1997 | 9231392 |
| characterization of hemolytic and cytotoxic gallysins: a relationship with arylphorins. | gallysin-1, an inducible effector protein in the protective response of galleria mellonella larvae is a 75 kda component of hemolytically active material (ham) isolated from immune cell-free hemolymph. the sequence of the first 20 n-terminal amino acids of the antibacterial protein gallysin-1 is identical to the predicted sequence of the first 20 amino acids of the galleria arylphorin lhp76 (larval hemolymph protein 76). a murine monoclonal antibody to the 20 amino acid n-terminal peptide of gal ... | 1997 | 9258607 |
| destruxin analogs: variations of the alpha-hydroxy acid side chain. | this work describes the synthesis of three destruxin e cyclodepsipeptidic analogs. these compounds have an identical amino acid sequence but differ by the nature of the hydroxy acid residue with is 2-hydroxy-3-phenylpropionic (hpp), 2-hydroxy-5-trimethylsilyl-4-pentynoic (hpy-tms) and 2-hydroxy-4-pentynoic (hpy) acid, respectively. the insecticidal properties on the galleria mellonella larvae (paralysis and lethal effect) of these analogs are presented in comparison with the natural destruxin e. ... | 1997 | 9273893 |
| isolation, inoculation to insect host, and molecular phylogeny of an entomogenous fungus paecilomyces tenuipes. | a parasitic fungus to moth larvae and pupae, paecilomyces tenuipes, was isolated and cultured on liquid and agar media. fruit bodies, or synnemata, with characteristics of p. tenuipes were successfully formed on the agar medium. when pupae of wax moth, galleria mellonella, were incubated with the conidia, all the pupae were infected and the synnemata were formed out of them. almost the entire length of 18s rdna of p. tenuipes was amplified by pcr and directly sequenced. molecular phylogenetic an ... | 1997 | 9367727 |
| comparison of endemic and exotic entomopathogenic nematode species for control of colorado potato beetle (coleoptera: chrysomelidae). | we compared the efficacy of 2 endemic strains of entomopathogenic nematodes isolated from hermiston, or, with that of 3 exotic nematode species for control of colorado potato beetle, leptinotarsa decemlineata (say). in laboratory experiments, the exotic heterorhabditis species were more pathogenic to colorado potato beetle than were the endemic heterorhabditis strains. exotic steinernema species were less pathogenic to colorado potato beetle than the exotic heterorhabditis species. no colorado p ... | 1997 | 9461847 |
| uv-difference and cd spectroscopy studies on juvenile hormone binding to its carrier protein. | juvenile hormone binding protein (jhbp) from hemolymph of galleria mellonella is a single-chain glycoprotein of molecular mass near 25,880 containing no trp residues. the fourth derivative of the protein absorption spectrum shows the characteristic vibrational components of the phenylalanine spectrum within the range 240-270 nm. at longer wavelengths two main bands 0-0 and 0+800 cm(-1) appear, caused by vibrational levels of electronic transition, pi-->pi*, in the tyrosine residues, with maxima ... | 1998 | 9516043 |