Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| theophylline inhibits early and late asthmatic reactions induced by allergens in asthmatic subjects. | to determine whether oral slow-release theophylline inhibits asthmatic reactions and the associated increase of airway responsiveness to methacholine induced by allergens, we examined six asthmatic subjects who developed a dual asthmatic reactions after allergen bronchoprovocation with dermatophagoides pteronyssinus or with grass pollen. we gave oral slow-release theophylline and placebo to each subject for seven days in two series of experiments in a double-blind, randomized, crossover study. t ... | 1991 | 2006773 |
| house dust mites in the west indies. | the purpose of this study was to determine the role of the house dust mite. dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df), in allergic diseases on the island of st. lucia. dust samples were taken from living quarters of patients and hospital volunteers. the level of df antigen in these living quarters was measured by rast inhibition. all samples showed df antigen within a large range of values. prick tests were done to house dust mite and molds on 91 patients with suspect ... | 1991 | 2014932 |
| comparison of fifteen production batches of house dust mite extract (dermatophagoides pteronyssinus). | an important measurement of quality of allergenic extracts is the batch to batch uniformity. using different methods of protein and immunobiochemical analysis, we could demonstrate, that 15 production batches of house dust mite dermatophagoides pteronyssinus extract prepared during the course of 4 years from different lots of the source material, (purified whole mite bodies) showed reproducible data. | 2000 | 2014936 |
| detection of b-lymphocytes secreting antibodies to dermatophagoides antigens. | an enzyme-linked immunospot assay (eli-spot assay) has been established to count individual cells secreting antibodies to dermatophagoides spp. allergens. initial optimization of the assay was performed using der p i-specific murine hybridoma cell lines. inhibition with soluble purified allergen demonstrated that spot formation was specific. addition of cycloheximide showed that spot formation was due to antibodies actively produced and secreted and not caused by preformed surface-associated ig. ... | 1991 | 2021882 |
| house dust acarofauna and der p i equivalent in australia: the relative importance of dermatophagoides pteronyssinus and euroglyphus maynei. | the acarofauna and der p i allergen concentrations in dust samples from mattresses and lounge room carpets obtained from 20 homes from two coastal cities. perth and bunbury, were determined. all samples were shown to contain mites and the geometric mean numbers of total mites/g of mattress and carpet dust for perth and bunbury were 480 and 263, and 585 and 992, respectively. carpets from both centres had a significantly (p less than 0.02) greater mean number of mite species (perth 9.1, bunbury 9 ... | 1991 | 2043990 |
| injection of allergen-antibody complexes is an effective treatment of atopic dermatitis. | atopic dermatitis (ad) can be exacerbated by contact with airborne allergens, amongst which dermatophagoides pteronyssinus (dpt) appears to be potentially important. specific ige antibodies towards dpt are often found in ad, and it can therefore be speculated that suppression of the production of anti-dpt ige might result in a significant clinical improvement. complexes of antigen and specific antibodies have been shown to suppress the production of antibody in other systems; we report here the ... | 1991 | 2050242 |
| [sensitization to granary mites in patients with allergic diseases]. | as many as 71 patients selected from a group of 550 patients suffering from allergic diseases, sensitized to the house dust mites dermatophagoides pteronyssinus were examined. a study was made of the presence of allergen-specific ige antibodies against house dust mites, storage mites (7 species altogether) using allergenic discs and commercial kits rast (pharmacia, sweden). the group under examination mostly manifested sensitization to the house dust mites: dermatophagoides ferinae (80%), eurogl ... | 1991 | 2063323 |
| [accelerated desensitization to dermatophagoides pteronyssinus mites in children with asthma. results of two years of immunotherapy]. | 16 children with chronic asthma were given a rush immunotherapy with standardized dermatophagoides pteronyssinus extracts. for two years, the dose of 10 ir was injected monthly. before this rush, six children have received a typical immunotherapy ineffective on asthma. examination of clinical, medication, immuno-allergologic and functional parameters were recorded before and at 6, 12 and 24 months of the follow-up. rush immunotherapy was well tolerated. substantial improvement of asthmatic syndr ... | 1991 | 2069687 |
| [ten years of experience with specific desensitization in children with bronchial asthma]. | hundred eighty five children, aged between 6 and 15 years, with bronchial asthma and diagnosed allergy to dermatophagoides pteronyssinus were followed-up for 2-3 years between 1978 and 1988. children were treated with hdm vaccine bencard. administered doses were increased gradually at the beginning of therapy whereas they remained the same for further 1-3 years. excellent and favourable results were achieved in 78.4% of cases. the treatment as postponed in 36 children (19.4%) because of the lack ... | 2008 | 2075131 |
| [results of skin tests 1981 to 1989]. | in the aim of proving the existence of organism sensitivity to standard group inhalation antigens, at the orl clinic in novi sad, in the period of 1981 through july, 1989, intradermal testing was conducted in 1816 patients. the greatest number of positive reactions appeared within the city inhabitants, mostly of female sex. most frequently they reacted to house dust (53.19%), pollen (37.88%), ambrosia (18.17%), dermatophagoides pteronissynus (11.17%), and much less frequently to other allergens. ... | 1990 | 2077378 |
| efficacy of sublingual immunotherapy in patients with rhinitis and asthma due to house dust mite. a double-blind study. | fifty eight patients under 12 years of age, positive to mites (dermatophagoides pteronyssinus and d. farinae) according to prick, "in vitro" specific ige and challenge tests, suffering from asthma and rhinitis, were randomly assigned on a double blind basis to receive per os either a biologically standardized extract of mites (active therapy ta = 30 patients) or a saline buffered solution (placebo = 28 patients). patients took sublingually increasing doses of the solution, followed by maintenanc ... | 2006 | 2097894 |
| [sensitivity to the primary house dust allergen--dermatophagoides pteronyssinus--in patients with atopic dermatitis]. | the study involved 117 adults and 535 children with atopic dermatitis. immunoglobulins e against d. pteronyssinus--main allergen of the home dust--were assayed with rast technique in children and fast technique in adult patients. it was found that the blood serum ige levels increase with patients' age and is the highest in patients with coexisting allergic respiratory diseases (difference statistically significant). rast precision was compared with that of "prick" skin tests in the detection of ... | 2008 | 2098737 |
| [the determination of allergen-specific ige and igg antibodies in patients sensitized to the house dust mite dermatophagoides pteronyssinus]. | 45 patients, hypersensitive to house-dust mites, were examined by the method of skin tests to d. pteronyssinus allergen. besides, in their blood sera the levels of allergen-specific ige antibodies were determined in the radioallergosorbent test and allergen-specific igg antibodies, in the enzyme immunoassay. these tests revealed that in 91% of the patients the results of skin tests were positive, in 68% an elevated level of specific ige antibodies and in 93% of the patients an elevated level of ... | 1990 | 2099077 |
| specific igg and ige responses to dermatophagoides pteronyssinus in sprague-dawley rats. | specific igg and ige antibodies against the mite dermatophagoides pteronyssinus (dp) have been determined in the serum of sprague dawley (sd) rats by enzyme linked immunosorbent assay (elisa) and passive cutaneous anaphylaxis (pca), respectively. immunization of rats with dp produces a rapid increase of serum specific ige antibody levels which decrease when specific igg antibodies start rising. the results indicate that the sd rat is a good model to induce a humoral immune response to allergen d ... | 1990 | 2099531 |
| [accelerated desensitization with dermatophagoides pteronyssinus in severe asthmatic children. evaluation after one year of immunotherapy]. | an homogeneous group of 17 children with severe chronic asthma were given a rush immunotherapy with standardized dermatophagoides pteronyssinus extracts. maintenance dose was injected monthly for one year. symptom and medication scores as well as functional (lung function tests, specific nasal challenges), immunologic and allergic parameters (skin tests, total serum ige, specific ige and igg4) were recorded before and at the end of the study. rush immunotherapy was well tolerated in spite of som ... | 1990 | 2111693 |
| expression of dermatophagoides pteronyssinus allergen, der p ii, in escherichia coli and the binding studies with human ige. | lambda gt11 clones expressing the major house dust mite allergen, der p ii, have been reported to react with ige in the serum of a high proportion of allergic patients. the clones described, however, only produced small quantities of protein which was not fused to the beta-galactosidase of the vector. a construct of the der p ii is described which produces a fusion of der p ii, minus its leader sequence, with the glutathione-s transferase in the pgex vector. this could be readily isolated and wa ... | 1990 | 2187817 |
| comparison between rast and pharmacia cap system: a new automated specific ige assay. | rast represents the standard technique to titrate serum-specific ige and was found to have a higher efficiency in the diagnosis of ige-mediated allergy than other in vitro tests. pharmacia cap system (cap) is a new solid-phase immunoassay, fully automated, used for the titration of specific ige antibodies. results are listed in kilounits per liter, equilibrated against the world health organization standard for ige. rast and cap were compared in 106 unselected patients (6 to 59 years) characteri ... | 1990 | 2191989 |
| comparison of a multi-allergen dipstick ige assay to skin-prick test and rast. | a multi-allergen dipstick enzyme immunoassay 'quidel allergy screen' (qas) has recently been developed commercially for measuring ige antibodies against nine allergens (house dust 1, house dust 2, dermatophagoides pteronyssinus, d. farinae, japanese cedar, ragweed, cat dander, sweet vernal grass, and egg white) at one time. to assess whether this assay is useful in screening allergen-specific ige antibody, we compared the titres of ige antibodies against the nine allergens measured by qas to tho ... | 1990 | 2192782 |
| the management of house dust mite allergies. | a safe and practical home sanitation procedure for the removal of house dust mites (dermatophagoides pteronyssinus) and their allergens is described. the severity of mite infestation was assessed with the use of the acarex test, which measures the concentration of guanine in house dust, and all affected house textiles were then treated with an acarosan product. the active ingredient of acarosan is benzyl benzoate, which has been used as a scabicide for over 100 years. the results of several stud ... | 2006 | 2199059 |
| [allergic individuals to japanese cedar or orchard grass consist of two subgroups based on the sensitization to dermatophagoides pteronyssinus]. | one hundred and twenty-six allergic subjects were divided into three groups based on the rast results for dermatophagoides pteronyssinus (d.p) and japanese cedar (j.c.). we compared sex, age of examination for rast, serum ige, family history of allergic diseases, prevalence rate of bronchial asthma (ba) and allergic rhinitis (ar), and onset of ar among three groups. no significant difference in the other variables except serum ige and prevalence rate of ba was found between the group with d.p-po ... | 1990 | 2222193 |
| effect of heat treatments on the house-dust mites dermatophagoides pteronyssinus and d. farinae (acari: pyroglyphidae) in a mattress-like polyurethane foam block. | the behaviour and survival of the house-dust mites dermatophagoides pteronyssinus and d. farinae were studied under laboratory conditions in a mattress-like polyurethane foam block that was heated by an electric blanket. mites obtained from a laboratory culture were introduced into the foam block which was sliced into seven horizontal layers and covered with an electric blanket. measurements of temperature and relative humidity were taken between the layers and the number of living mites was est ... | 1990 | 2226071 |
| the control of house dust mite allergens in rugs. | used rugs were vacuum cleaned, "wet cleaned," "shampooed," or heated in an autoclave. the effects on the live-mite population, on the allergen (dermatophagoides pteronyssinus, der p i and der p ii; and dermatophagoides farinae, der f ii) content, and on the suitability of the rugs as a habitat for house dust mites was studied. autoclaving was most effective in all three aspects. the cleaning methods were quite inefficient for killing the mites, but a significant reduction of allergens and deteri ... | 1990 | 2229844 |
| temporal association between der pi exposure, immediate hypersensitivity and clinical severity of eczema. | uncontrolled studies of avoidance of house dust mite (dermatophagoides pteronyssinus) by atopic subjects have reported clinical improvement in the severity of eczema. the aim of the present study was to examine the temporal relationship between environmental exposure to the major mite allergen (der pi) and clinical disease severity in children with chronic atopic eczema. twelve children were identified as being house dust mite-sensitive on the basis of skin prick test and rast to der pi. they we ... | 1990 | 2244674 |
| antigenic analysis of group i house dust mite allergens using random fragments of der p i expressed by recombinant dna libraries. | antigenic regions of a major house dust mite allergen, der p i, were identified by a recombinant dna strategy employing the technique of random fragmentation. fragments of cdna coding for der p i were produced by sonication and used to construct lambda gt 11 expression libraries. analyses of recombinant fragments reactive with a rabbit anti-der p i antiserum showed that the b cell determinants expressed in escherichia coli were limited, with the majority (86%) of antigenic clones isolated mappin ... | 1990 | 2246073 |
| a double-blind study of hyposensitization with an alginate-conjugated extract of dermatophagoides pteronyssinus (conjuvac) in patients with perennial rhinitis. ii. immunological aspects. | in a 2-year double-blind placebo controlled study an immunological evaluation was carried out on 33 patients (15 males, 18 females, mean age 29.2 years) with mite-induced perennial rhinitis who were submitted to specific immunotherapy (it) with an alginate-conjugated extract of d. pteronyssinus. the behaviour of ige, igg, igg1 and igg4 antibodies specific to d. pteronyssinus and its major allergen der p1 was characterized by assessment of their changes in serum, and changes in igg in nasal secre ... | 1990 | 2252161 |
| [effect of specific desensitization therapy in combination with an immunopotentiator, neurotropin, on perennial nasal allergy--with special reference to mite specific igg and igg4 antibody levels]. | we treated perennial nasal allergy with specific desensitization in combination with or without neurotropin injections (nsp) from march 1983 to september 1984, and analyzed blood anti-mite igg and igg4 levels in relation to therapeutic efficacy. desensitization and nsp were given once a week and seventy subjects (33 from the desensitization group and 37 from the desensitization + nsp group) were evaluated at 24, 36 and 48 weeks. 1) in total cases clinical usefulness showed no significant differe ... | 1990 | 2260931 |
| topical nasal immunotherapy. immunologic response of ige in nasal secretion after immunotherapy with dermatophagoides pteronyssinus. | we studied 9 children between 9 and 14 years old who had been diagnosed with exclusive allergic rhinitis and were sensitive to acari. topical nasal immunotherapy for d. pteronyssinus was carried out for 2 years. the total ige in nasal secretions measured before immunotherapy showed levels of 14.2 to 85.2 iu/ml, and the specific ige in nasal secretion against d. pteronyssinus and d. farinae ranged from 0.6 to 8.86 pru (classes ii and iii). after the immunotherapy the total ige in nasal secretion ... | 2004 | 2264553 |
| [results of patch test using mite components in atopic dermatitis (ad) patients. 1st report. "as is patch test" using crushed mites]. | as is patch test using crushed live mites and crushed dried dead mites of dermatophagoides pteronyssinus (d.p.), dermatophagoides farinae (d.f.), & tryophagus ptrescentiae (t.p.) was performed on 49 ad patients and 18 control patients suffering from dermatitis other than ad. results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 ad patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases ... | 1990 | 2273575 |
| [result of "as is patch test" using mite elements in atopic dermatitis (ad) patient. 2nd report, "mite lipid"]. | the antigenicity of mite lipid was studied, taking atopic dermatitis as an allergic disease. lipid was extracted from dermatophagoides pteronyssinus (dp) and d. farinae (df). it was tentatively designated a mite lipid antigen (dp-l, df-l), and then a patch test was performed. an extract from animal food (lc) and acetone were used as the controls. with only dp-l, a positive reaction of more than one plus (+) of the icdrg standard was noted in 3 out of 21 cases of ad. histology of the above-mentio ... | 1990 | 2273576 |
| development of dermatophagoides pteronyssinus (acari: pyroglyphidae). | developmental times (egg to adult) and fecundity of the european house dust mite, dermatophagoides pteronyssinus (trouessart), were determined for various temperatures that normally occur in the mite microhabitat in homes. egg-to-adult developmental times were 122.8 +/- 14.5, 34.0 +/- 5.9, 19.3 +/- 2.5, and 15.0 +/- 2.0 d (means +/- sd) at 16 (61 degrees f), 23 (73 degrees f), 30 (86 degrees f), and 35 degrees c (95 degrees f), respectively. developmental times of individual life stages were con ... | 1990 | 2280388 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| the spectrum of low molecular weight house dust mite (dermatophagoides pteronyssinus) allergens with emphasis on der p ii. | analysis by protein blotting of sera from 96 different house dust mite-allergic subjects revealed previously unrecognized complexity of low molecular weight (mw) (less than 20 kd) ige-binding proteins in extracts of whole bodies of dermatophagoides pteronyssinus. of 11 different ige-binding components of mw less than 20 kd identified, two (mw approximately 16 kd and approximately 15 kd), showed both a high frequency (88% and 49% respectively) and a high intensity of ige-binding. the approximatel ... | 1990 | 2310979 |
| the prevalence of allergic disease and ige antibodies to house dust mite in schoolchildren in taiwan. | the prevalence of positive specific ige antibodies to house dust mites (dermatophagoides pteronyssinus; d. farinae) was determined by enzyme-linked immunosorbent assay (elisa) in 5097 (61%) volunteers of 8345 schoolchildren aged between 7 and 14 yr from two government schools. all of them filled out a questionnaire concerning allergic symptoms. among them, 412 (8.1%) children showed a positive reaction to at least one of the two mite allergens, the range varying between 5.6 and 11.2% according t ... | 1990 | 2310980 |
| hyposensitization in asthmatics with mpeg-modified and unmodified house dust mite extract. iv. occurrence and prediction of side effects. | a double-blind study on hyposensitization (hs) with two extracts prepared from the house dust mite dermatophagoides pteronyssinus (dp) was performed on a group of asthmatics with bronchial sensitivity to dp. in 18 patients, aluminium-hydroxide was added to the dp-extract to give a depot effect (dp-group). nineteen patients were treated with a similar extract in which allergenicity had been reduced by coupling to monomethoxypolyethylene glycol (mpeg-dp-group). this extract had previously been sho ... | 1990 | 2316824 |
| allergic bronchial asthma due to dermatophagoides pteronyssinus hypersensitivity can be efficiently treated by inoculation of allergen-antibody complexes. | antigen-antibody complexes were made from allergens of the common house dust mite, dermatophagoides pteronyssinus (dpt) and an excess of purified autologous specific antibodies. these complexes have been used to treat dpt-hypersensitive patients who suffered from chronic bronchial asthma. clinical symptoms and medication intake were followed by filling in diary cards. peak expiratory flow, measured four times a day, was also followed. intradermal skin tests and bronchial challenge tests were per ... | 1990 | 2318962 |
| [seasonal variations in house dust mite allergens--significance for children with allergy]. | allergenic proteins of the house dust mites dermatophagoides pteronyssinus and dermatophagoides farinae and their major allergens der p i and der f i are one of the most significant environmental factors which may facilitate sensitization and allergic airway disease in childhood. in order to measure seasonal variations of mite allergen exposure, we collected mattress dust samples monthly in 13 homes over a period of 1 year. der p i and der f i were determined with a sandwich type elisa. mite all ... | 1990 | 2320013 |
| dermatophagoides and storage mites: statistical analysis of rast results. | r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity. | 1990 | 2321810 |
| the effect of inhaled fenoterol, administered during the late asthmatic reaction to house dust mite (dermatophagoides pteronyssinus). | a double-blind, placebo-controlled crossover study was set up to investigate the effect of fenoterol (400 micrograms) during the late asthmatic reaction (lar). twenty young subjects with asthma (mean age, 11.8 years; range, 8.3 to 20.6 years) were selected on the basis that they developed an lar after bronchial challenge with dermatophagoides pteronyssinus. after the lar occurred, a placebo and fenoterol were administered blindly by a metered-dose inhaler, with an interval of 15 minutes and in a ... | 1990 | 2324409 |
| who will benefit from hyposensitization? predictive parameters in house dust mite allergic asthmatics. | the aim of this study was to assess the ability of various data collected before treatment to predict the therapeutic benefits of hyposensitization. thirty-one asthmatics were hyposensitized with extract from the house dust mite dermatophagoides pteronyssinus (dp) for 2 years, 15 comparable patients served as controls. the treatment extract was either modified by coupling to monomethoxypolyethylene glycol (mpeg) or administered in a diluent containing al(oh)3. improvement would be either a great ... | 1990 | 2327561 |
| localization of antigens and allergens in thin sections of the house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae). | cryostat sections of the house dust mite, dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, der p i. sections also were probed for allergens with sera from human mite-allergic subjects containing ige antibodies to mite allergens and with lectins. antibody binding was mainly to the gut lining and gut contents of the mite, although some specific labeling also ... | 1990 | 2332879 |
| isolation of cdna coding for the major mite allergen der p ii by ige plaque immunoassay. | a lambda gt11 library made with cdna from the house dust mite dermatophagoides pteronyssinus was screened with human allergic serum by ige plaque radioimmunoassay. this resulted in the isolation of clones coding for the major allergen der p ii. the cdna coded for a 129-residue protein of 14,131 daltons with no n-glycosylation sites. no sequence homology with other proteins was evident. the der p ii expressed in escherichia coli reacted with ige in 14 of 17 sera from mite-allergic patients giving ... | 1990 | 2341191 |
| immune response to blomia kulagini and dermatophagoides pteronyssinus in sweden and brazil. | the immune responses to the non-pyroglyphid mite blomia kulagini and the pyroglyphid mite dermatophagoides pteronyssinus were compared in 440 swedish farmers and 34 allergic subjects from brazil using the radioallergosorbent test (rast). both b. kulagini and d. pteronyssinus were shown to be a common cause of sensitization, particularly in brazil. the highest rast scores were found against d. pteronyssinus in sera from both countries. allergenic cross-reactivity between the two mites was studied ... | 1990 | 2341199 |
| [the fauna, number and spatial distribution of mites (acari) in house dust in moscow]. | there is a wide and strong allergenic background in moscow, which is determined by relatively high population density and regular frequency of the allergenic mites in various premises. 34 species have been identified, 5 of them are allergen producers. the mites of the family pyroglyphidae are the main component of the house dust fauna. dermatophagoides pteronyssinus and d. farinae distributed all over the world are predominate. a comparative evaluation of the fauna structure, numbers and frequen ... | 1990 | 2346769 |
| a study of the relationship between childhood nephrotic syndrome and allergic diseases. | the incidence of atopic diseases in 206 children with nephrotic syndrome (ns) was studied. boys with ns had three times higher incidence of bronchial asthma than the general population. there was no difference in the girls. both boys and girls with ns had about three times more allergic rhinitis and ten times more atopic dermatitis than the general population. in ns patients with associated allergic disease, skin test and allergen-specific ige antibodies by radioallergosorbent test (rast) were p ... | 1990 | 2347227 |
| changes of serum-specific ige antibody titer during hyposensitization in mite-sensitive asthmatic children. | in this study, we used the enzyme-linked immunosorbent assay (elisa) to evaluate the changes of ige antibody titer against dermatophagoides pteronyssinus (d. pteronyssinus) or dermatophagoides farinae (d. farinae) in asthmatic children after immunotherapy. according to elisa analysis, a significantly higher mean level of ige antibody titer to d. pteronyssinus (or d. farinae) was found in nonhyposensitized asthmatic children than in the pediatric control group (p less than 0.001), but there was n ... | 1990 | 2347816 |
| the use of phadiatop in mass-screening programmes of inhalant allergies: advantages and limitations. | the validity of phadiatop as a tool in the mass screening for inhalant allergies was investigated. seventy-nine out of 600 recruits (13.2%) were classified as allergic to inhalant allergens (68 oculorhinitis; 11 asthma) on the basis of positive history, confirmed by skin testing and/or rast for the seven most common aero-allergens in italy. another 74 subjects had positive rast and/or skin tests, yet had never experienced allergic symptoms. phadiatop was positive in 145/600: in 78 out of 79 alle ... | 1990 | 2357615 |
| ecology of house dust mites in oxfordshire. | in a survey of the mite fauna in 30 homes in oxfordshire, dermatophagoides pteronyssinus and euroglyphus maynei were found to be the most abundant species but d. farinae was absent. of a total of 14 parameters investigated for their influence on mite numbers, relative humidity was apparently the most important factor. bedrooms with humidities above 64% contained significantly more mites in mattresses than those with lower humidities. bedroom humidity and thus mattress mite numbers were apparentl ... | 1990 | 2357619 |
| house dust mite sensitivity: a comparison of immune response with exposure to four species of pyroglyphidae. | twenty-five atopic children under 11 years of age were studied, using skin and rast tests, for their specific ige response to four species of pyroglyphid house dust mites, dermatophagoides pteronyssinus, d. farinae, d. microceras and euroglyphus maynei. all of the children were sensitive to d. pteronyssinus, 20 (80%) of these children were also sensitive to d. farinae and d. microceras, and 16 of the latter (64%) were also sensitive to e. maynei. dust samples from various sites in the homes of t ... | 1990 | 2364312 |
| assessment and control of house dust mite infestation. | most allergens of the house dust mite (dermatophagoides pteronyssinus and dermatophagoides farinae) are carried by the mite excreta rather than by the mites themselves. a method of assessing the concentration of mite excreta in dust (the acarex test) is described. the effects of acarosan products, whose active ingredient is solidified benzyl benzoate, on mites and mite excreta were evaluated. over a period of three years, three to four applications of acarosan effectively destroyed mites in carp ... | 1990 | 2379225 |
| functional characterization of lymphocyte response to fractionated house dust mite antigens (dermatophagoides pteronyssinus) in atopic and non-atopic individuals. | this study investigated the cellular response of human lymphocytes to dermatophagoides pteronyssinus crude antigen and 14 molecular weight (mw) fractions. the cells were derived from atopic patients and healthy individuals who were skin test-positive or skin test-negative to mite antigen. when stimulated with crude antigen, the group of patients showed elevated proliferation and production of lymphokine in comparison with the healthy skin test-negative individuals (p less than 0.01). by stimulat ... | 1990 | 2379943 |
| evolution of the late asthmatic reaction during immunotherapy and after stopping immunotherapy. | in previous studies it was demonstrated that the frequency and the severity of the late asthmatic reaction (lar) can be attenuated by immunotherapy (it). the present study was set up to observe the evolution of the lar under it and after having stopped it. nineteen children with bronchial asthma and an lar to house dust mite (dermatophagoides pteronyssinus) (hdm) were selected for this study. all subjects received it with hdm extract during 1 year. thereafter, the children were divided randomly ... | 1990 | 2384645 |
| comparison of diversity and function of house dust mite-specific t lymphocyte clones from atopic and non-atopic donors. | panels of cd4+cd8- t lymphocyte clones (tlc), specific for house dust mite dermatophagoides pteronyssinus (dp) proteins, were generated from the peripheral blood of an atopic dp-allergic donor (ad), suffering from severe atopic dermatitis, and a histocompatible non-atopic donor (nad). we studied the diversity of tlc within these two panels in search for the possible occurrence of dominant clone types with properties that might be characteristic for the atopic or non-atopic state. tlc with specif ... | 1990 | 2387315 |
| immunotherapy with a standardized dermatophagoides pteronyssinus extract. iv. systemic reactions according to the immunotherapy schedule. | specific immunotherapy with standardized extracts can induce systemic reactions (srs), possibly increased by a rush immunotherapy (rit) protocol. a prospective study in 1152 mite-allergic patients (3 to 63 years of age) examined the incidence of srs during an rit or a step protocol. all patients received the same standardized extract of dermatophagoides pteronyssinus with the same maintenance dose. in the first group, 290 patients had an rit protocol without any preventive measure. in a second g ... | 1990 | 2406324 |
| [the histamine liberation test as a diagnostic parameter and its possible modification following specific immunotherapy]. | it is generally accepted that the allergic reaction depends on the levels of specific ige, on the concentration of blocking antibodies and on the capacity of the basophils to release mediators. the fact that in the histamine release test (hrt) all of the elements intervening in an allergic reaction (antigens, ige, basophils and mononuclear cells of a lymphoid origin) have been found, has led some authors to consider it as the most suitable method of "in vitro" diagnosis. this is a reflection of ... | 1985 | 2408457 |
| activity of allergenic proteins from dermatophagoides pteronyssinus. comparison of crossed radioimmunoelectrophoresis (crie) and histamine release from washed leukocytes. | two purified allergens from dermatophagoides pteronyssinus, dp 42 (identical to p1) and dp x were studied for their ability to induce histamine release from washed leukocytes and to bind to ige antibodies from the serum of 27 mite-sensitive children. almost all patients were demonstrated to be sensitive to both proteins by both assays. dp 42 was found to have the highest allergenic activity, releasing histamine from leukocytes at a median concentration 10 times lower than for dp x. there was a p ... | 1985 | 2413784 |
| purification and partial characterization of two major allergens from the house dust mite dermatophagoides pteronyssinus. | two major allergens of the house dust mite, dermatophagoides pteronyssinus (dp), were purified, and their molecular weight and isoelectric points (pis) were determined. dp 42 was purified from an acetone-precipitated mite-excrement extract by a combination of hydrophobic interaction chromatography on phenyl sepharose and copper-chelate chromatography. the molecular weight was determined to be 18,000 and 25,000 to 30,000 by gel filtration (g-75) and sodium dodecyl sulphate-polyacrylamide gel elec ... | 1985 | 2414353 |
| studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea. | the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ... | 1986 | 2420852 |
| [changes in histamine liberation induced by house dust mites after the administration of procaterol]. | the number of patients clinically suffering from rhinitis and/or asthma is very important in any collective of our society. the usage of drugs with a bronchodilator effect as a symptomatic therapy has been widely studied. however, the appearance of new products which complements the effect of bronchodilator and antianaphylactic union has not been sufficiently studied. the motive of this study is to evaluate the inhibition of histamine as the primary and principal mediator, liberated specifically ... | 2013 | 2424293 |
| cross-reactivity between the house dust mite dermatophagoides pteronyssinus and the mange mites psoroptes cuniculi and p. ovis. i. demonstration of antibodies to the house dust mite allergen dpt 12 in sera from p. cuniculi-infested rabbits. | the cross-reactivity of the mange mites psoroptes cuniculi (pc) and psoroptes ovis (po) antigens with the house dust mite dermatophagoides pteronyssinus (dp) antigens has been studied. cross-reactivity between mange mite and house dust mite antigens was demonstrated by both elisa and immunoelectrophoresis by use of a sheep anti-dp antiserum. both pc and po were demonstrated to contain eight cross-reacting antigens. sera from rabbits infested with pc were demonstrated to produce antibodies to the ... | 1986 | 2426321 |
| cross-reacting and species-specific determinants on a major allergen from dermatophagoides pteronyssinus and d. farinae: development of a radioimmunoassay for antigen p1 equivalent in house dust and dust mite extracts. | two species of mites of the genus dermatophagoides are common in house dust and make a major contribution to the allergen content of house dust. as judged by skin tests and the radioallergosorbent test, these mites, d. pteronyssinus and d. farinae, demonstrate extensive cross-reactivity. when the major allergen from d. pteronyssinus, antigen p1, was compared with the equivalent allergen from d. farinae, these two proteins were found to have both species-specific and common antigenic determinants ... | 1986 | 2428855 |
| antigen der f i from the dust mite dermatophagoides farinae: structural comparison with der p i from dermatophagoides pteronyssinus and epitope specificity of murine igg and human ige antibodies. | the physicochemical and antigenic properties of an allergen purified from dermatophagoides farinae, der f i, were compared with der p i from dermatophagoides pteronyssinus. on sds-page, der f i migrated as a single polypeptide chain with the same m.w. as der p i (24,000). two isoallergenic peaks of der f i were identified on preparative isoelectric focusing (pi 5.7 to 6.3 and pi 6.6 to 6.95). fractions from each peak were shown to have an identical amino acid composition (which was similar but n ... | 1986 | 2428875 |
| comparison by electroblotting of ige-binding components in extracts of house dust mite bodies and spent mite culture. | eight dermatophagoides pteronyssinus extracts (three culture extracts, four mite body extracts, and the world health organization international standard [is]) were investigated by side-by-side sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by electrotransfer to nitrocellulose, and by probing with a pooled serum from mite-allergic subjects. representative body and mite culture extracts were compared by probing with individual sera, and both types of extract were also compared by rast- ... | 1987 | 2433323 |
| investigations of culture medium-free house dust mites. iv. cross antigenicity and allergenicity between the house dust mites, dermatophagoides farinae and d. pteronyssinus. | heterologous crossed immunoelectrophoresis (cie) analysis demonstrated that dermatophagoides pteronyssinus body and d. farinae body extracts contained up to 21 cross-reacting antigens (ags). incubation of cie gels with two mite-sensitive individual sera and a serum pool of mite-sensitive patients indicated that up to nine cross-reacting ags bound mite-specific ige on crossed radioimmunoelectrophoresis and were common allergens. likewise, heterologous cie analysis of d. pteronyssinus feces and d. ... | 1987 | 2434550 |
| selection and characterization of monoclonal antibodies against a major allergen in dermatophagoides pteronyssinus. species-specific and common epitopes in three dermatophagoides species. | hybridomas were produced after immunization with der p i (ag 42, p1). specific anti-der p i activity was detected in a reverse elisa using immobilized anti-mouse ig and highly purified der p i labelled with horseradish peroxidase. stable subclones from 6 parental hybridomas were obtained. the species specificities of the monoclonal antibodies (mabs) were classified: by an overlayer technique analogous to the crie technique, and by two competitive elisa techniques. one mab recognized an epitope s ... | 1987 | 2440816 |
| epitope mapping of two major inhalant allergens, der p i and der f i, from mites of the genus dermatophagoides. | the repertoire of antigenic sites on two major dust mite allergens, der p i of dermatophagoides pteronyssinus and der f i of d. farinae, was studied using murine (balb/c) monoclonal antibodies (mab), polyclonal rabbit igg antibodies, and human ige antibodies. fifty-three igg mab were analyzed from six different fusions (five vs der p i, one vs der f i). by antigen binding radioimmunoassay (ria), most mab were either der p i or der f i specific, and only 2/53 bound to both allergens. epitope mapp ... | 1987 | 2442247 |
| [specific ige antibody against dermatophagoides pteronyssinus in nasal secretion and nasal mucosa of asthmatic children with subclinical nasal allergy]. | 2008 | 2442336 | |
| house dust mite asthma. correlation between allergen sensitivity in various organs. | fifty asthmatics, candidates for hyposensitization with the house dust mite dermatophagoides pteronyssinus (dp), went through a series of allergy tests to evaluate the sensitivity of different organs to dp. all patients were exposed to bronchial challenge with histamine and bronchial, nasal and conjunctival challenge with dp, skin prick test (spt) with dp, analyses for dp-specific histamine release from blood cells (hr) and for anti-dp-ige in serum (rast). results from 40 patients reacting posit ... | 1987 | 2444129 |
| cloned human t lymphocytes reactive with dermatophagoides farinae (house dust mite): a comparison of t- and b-cell antigen recognition. | in this report, t-cell and b-cell recognition of the house dust mite dermatophagoides farinae (d. far.) is compared. nitrocellulose immunoblots of polyacrylamide gel electrophoresis (sds-page)-fractionated d. far. were added to proliferation assays to map the antigen specificity of cloned human helper t cells and a long-term line induced with d. far. t-cell recognition was of a polypeptide of molecular weight 9000-13,000, that migrates with the serologically defined allergen der fii (12,500 mw). ... | 1987 | 2448229 |
| modification of house dust mite allergens by monomethoxypolyethylene glycol. allergenicity measured by in vitro and in vivo methods. | in animal models, allergen modification by coupling to monomethoxypolyethylene glycol (mpeg) molecules can reduce allergenicity of the extract and makes the allergen capable of suppressing boosted ige response. to investigate in a human system the degree of attenuation implied by a mpeg modification of a house dust mite (dermatophagoides pteronyssinus) extract, 55 adults with asthma caused by house dust mites were tested by skin prick test (spt) and histamine release assay (hr). rast inhibition ... | 1988 | 2448247 |
| atopic dermatitis, house dust mite allergy and month of birth. | an analysis of the month of birth in 210 patients with atopic dermatitis and a positive prick test towards the house dust mite (dermatophagoides pteronyssinus) showed a significantly higher prevalence of birth in the interval may to november when compared with the expected distribution (p less than 0.01). a subdivision of the ad patients due to the concomitant presence of asthma showed no differences as the relative risk was nearly the same in the two subgroups. this observation indicates that a ... | 1987 | 2449000 |
| passive sensitization of basophil leukocytes from non-atopic adult by plasma from allergic children. | basophil leukocytes from a non-atopic donor, who responded well to anti-ige, were depleted of their native membrane-bound ige by acid treatment and passively sensitized with plasma containing either phleum pratense-, dermatophagoides pteronyssinus- or dog dander- specific ige obtained from 18 allergic children. histamine release was then performed on the passively sensitized cells and the results were compared with those of bronchial provocation test (bpt), allergen-specific serum ige (rast), sk ... | 1988 | 2449829 |
| skin tests and histamine release with p1-depleted dermatophagoides pteronyssinus body extracts and purified p1. | monoclonal antibodies were raised against p1 and dp x, two major allergens in dermatophagoides pteronyssinus extracts. the concentrations of ige antibodies to p1 and dp x in sera of mite-sensitive patients were determined in rast with immunosorbent-bound monoclonal antibodies used for insolubilization of the allergens. the major allergen-specific ige titers were compared with the total ige response against d. pteronyssinus. the results of these serologic assays confirmed studies in the literatur ... | 1988 | 2453544 |
| latex allergosorbent test (last): a new immunoassay for specific ige with latex particles. | we describe a two-step latex (lx) agglutination assay for the titration of specific anti-dermatophagoides pteronyssinus ige. the samples are first incubated with allergen-coated lx of 2.3 microns diameter. bound ige is digested by pepsin and then titrated by its agglutinating activity on 0.8 micron lx particles coated with antihuman fc epsilon rabbit f(ab')2. this latex allergosorbent test detects 100 pg of specific ige per milliliter and does not depend on the concentration of total ige. owing ... | 1988 | 2455743 |
| [long-term tolerance of specific hyposensitization with the aid of calcium phosphate-adsorbed mite extracts]. | 90 children receive a specific hyposensitization with calcium phosphate adjuvant purified dermatophagoides pteronyssinus allergens. we note 44% very good results, 37% good results and 19% failures. we propose to begin with 1/1,000,000 concentration every other day until 1/10,000 concentration is obtained under antihistamine protection. tolerance is satisfactory: moderate respiratory reaction are pointed out for 22 patients. no anaphylactic shock is encountered. for 80 children with more than thr ... | 1986 | 2456078 |
| release of highly-sulphated glycosaminoglycans and histamine from human basophils. | a new assay for highly-sulphated glycosaminoglycans (gags) has been applied to the study of allergen (dermatophagoides pteronyssinus)-induced release of gag from human basophils. highly-sulphated gag, which was not heparin, was found only in basophil-containing cellular fractions of whole blood. its release, like that of histamine, was ca++-dependent. however, allergen-induced release of gag from the basophils of atopic individuals exhibited a different time-course and dose-response from that of ... | 1988 | 2456991 |
| role of igg4 in histamine release from human basophil leucocytes. i. sensitization of cells from normal donors. | several conflicting reports on the ability of igg4 to mediate type i allergic reactions have appeared lately. we have developed a model system for testing this possibility, using passive sensitization of basophil leucocytes from normal individuals. at first, the system was optimized with regard to donor cells and anti-igg4 antibody: among 3 normal individuals with cells showing high, medium and low responses to anti-ige, only the high responder showed a reproducible, but low response to anti-igg ... | 1988 | 2457556 |
| guanine and mite allergenicity in house dust. | guanine is the major nitrogenous waste product in arachnids. it may serve as an indicator for allergenic mite faecal pellets. the present study assesses the correlation between the mit allergenicity of different house dust (hd) samples and their guanine content. guanine content in hd samples was evaluated either by the acarex-test or determined quantitatively, especially for in-vitro methods. using intradermal tests in selectively sensitized dermatophagoides pteronyssinus (dpt) patients, we eval ... | 1988 | 2458199 |
| mhc class ii restriction specificity of cloned human t lymphocytes reactive with dermatophagoides farinae (house dust mite). | in this report the antigen and restriction specificity of human t-cell clones induced with dermatophagoides farinae (d. farinae) and isolated from an atopic individual with perennial rhinitis has been investigated. of the six clones analysed, four were species specific and two showed cross-reactivity for the closely related dermatophagoides pteronyssinus (d. pteronyssinus). inhibition of antigen-dependent proliferation by murine monoclonal antibodies directed against hla-d-region gene products r ... | 1988 | 2459050 |
| occurrence and subclass distribution of igg antibodies to dietary antigens in children with atopic dermatitis and in their mothers. | occurrence and subclass distribution of igg to alpha-lactalbumin (ala), beta-lactoglobulin (blg), and ovalbumin (oa) have been determined in 30 children with atopic dermatitis (ad) and in their mothers. thirty nonatopic (na) children and their mothers were studied as controls. total ige and ige to ala, blg, oa, dermatophagoides pteronyssinus, and lolium perenne were also investigated. the main purpose of this study was to determine whether in humans, as observed in animals, maternal allergen-spe ... | 1988 | 2459993 |
| loratadine, an antihistamine, blocks antigen-and ionophore-induced leukotriene release from human lung in vitro. | some h1-antihistamines possess anti-allergic properties, and inhibit the immunological release of mediators including histamine and sulfiopeptide-leukotrienes (slow reacting substance of anaphylaxis) from lung. the effects of the antihistiamine loratadine, sch29851, on the release of leukotrienes and histamine from human lung fragments were measured, using the calcium ionophore a23187 and an extract of antigen from dermatophagoides pteronyssinus, house dust mite, (with passively sensitized lung) ... | 1988 | 2470117 |
| strong delayed-type hypersensitivity induced against house dust mite antigens in the mice. | the aim of this study was to assess the antigenicity of house dust mite antigens using delayed-type hypersensitivity (dth) responses in mice. the crude extracts of dermatophagoides pteronyssinus and dermatophagoides farinae induced strong dth responses and was transferred with t cells. assessment of dth responses to the house dust revealed that substantial cross-reactivity at t-cell level was observed between these two mite extracts. almost all the fractions of the extract of d. pteronyssinus, w ... | 1989 | 2470689 |
| "in vivo" and "in vitro" evaluation of four antihistamines (astemizole, azatadine, mequitazine, terfenadine). | a group of 32 patients with perennial allergic rhinitis sensitized exclusively to house dust and mites were evaluated for "in vivo" modifications (skin tests for histamine, house dust and mites) and "in vitro" alterations (seric ige, specific ige, histamine release curve to different concentrations of dermatophagoides pteronyssinus and histamine release curve to this pneumoallergen after incubation with h1 receptor antagonists) to four antihistamines (astemizole, azatadine, mequitazine and terfe ... | 1966 | 2476015 |
| high mite-allergen exposure increases the risk of sensitization in atopic children and young adults. | concentrations of major allergens of dermatophagoides pteronyssinus (dpt) and d. farinae (df), der p i and der f i, were determined in 183 dust samples of mattresses of 133 atopic and 50 nonatopic children by a sandwich-type elisa. atopic children and young adults living in houses with high levels of der p i and der f i (greater than or equal to 2000 ng/gm of dust) were found to have significantly higher serum ige levels to dpt and df (p less than 0.0001) compared to patients with low mite-aller ... | 1989 | 2478606 |
| evaluation of immune parameters (including specific igg4) during immunotherapy. | a group of 26 patients with allergic asthma and rhinitis, exclusively sensitized to house dust and mites, underwent immunotherapy with an extract of dermatophagoides pteronyssinus (allpyral) for a period of 36 months. every 6 months we carried out a clinical examination and assessed ige levels, specific ige to house dust and mites, histamine release curve to house dust and mites, cd3+ cd4+ cd8+ lymphocyte subpopulations, anti-dermatophagoides pteronyssinus specific igg and anti-dermatophagoides ... | 2006 | 2479244 |
| basophil degranulation test in house dust mite allergy: diagnostic value. | after reviewing the experience of various authors we applied the basophil degranulation test (bdt) in the study of sensitization to dermatophagoides pteronyssinus, so as to evaluate its diagnostic use. fifty-five patients with asthma and/or rhinitis and a positive skin test to the mentioned antigen, as well as 20 control subjects were studied. the results demonstrated a sensitivity of 65.5% and a specificity of 80% with an efficiency index of 69.5%. we did not find any significant correlation wi ... | 2004 | 2479246 |
| comparative analysis of physicochemical and immunochemical properties of the two major allergens from dermatophagoides pteronyssinus and the corresponding allergens from dermatophagoides farinae. | two major allergens, dp1 (der p i) and dp2 (der p ii), were isolated from the whole culture extract of dermatophagoides pteronyssinus, and the physicochemical and immunochemical properties of these allergens were compared with those of the corresponding allergens from dermatophagoides farinae, df1 (der fi) and df2 (der fii). on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, polyacrylamide gel isoelectric focusing and amino acid analysis, both dp1 and dp2 were demonstrated to have clo ... | 1989 | 2498214 |
| house dust mites in naval ships, military barracks, and homes in the hampton roads area of virginia. | this survey was undertaken to determine the possible exposure of military personnel to house dust mites in ships, barracks, and homes in the hampton roads area of virginia. during the period from april 1, 1982 through august 31, 1983, 524 dust samples were collected with vacuum cleaners. mites were extracted from aliquots of dust, counted, and identified as to species and life stage. dermatophagoides pterynssinus (european house dust mite) was more common in private homes, while dermatophagoides ... | 1989 | 2507967 |
| pyroglyphid mites in the high tatras sanatoria. | 1. in our work we studied the problematics of house dust fauna in the sanatoria and institutes of the tatra region, regarding especially the incidence of mites of the family pyroglyphidae that are considered for the major source of house dust allergen. 2. in eleven places of different altitude in this region, there were in 1982 chosen 13 localities--sanatoria and institutes. here, once quarterly, were collected house dust samples from beds and floors. 3. in the locality 1 helios, strbské pleso, ... | 1989 | 2519180 |
| [pathology caused by acari]. | this report concerns the observations the authors have been carrying out for over twelve years in sardinia; they regard the sensitization to house dust mites (dermatophagoides pteronyssinus, dermatophagoides farinae) and to storage mites (acarus siro, glycyphagus domesticus, lepidoglyphus destructor, tyrophagus putrescentiae) and are been obtained with: 1) skin tests with mite extracts; 2) radioallergosorbenttest (rast) for specific ige and igg; 3) mite exposure in homes and mite analysis; 4) hy ... | 1989 | 2519754 |
| effect of cytokines on spontaneous and allergen-induced cd23 expression, scd23 release and ig(e,g) synthesis from peripheral blood lymphocytes. | we studied the expression of the cd23 antigen as well as the release of soluble cd23 from peripheral blood lymphocytes (pbl) of atopic donors. pbl were stimulated with allergen (dermatophagoides pteronyssinus; der.p.), cytokines (interleukin-4, interferon-gamma; il-4, ifn-gamma) or combinations of stimuli. cd23 levels were enhanced after the addition of allergen or il-4 (10 u/ml) up to 10-fold compared to spontaneous expression. soluble cd23 (scd23) release was enhanced in a dose-dependent manne ... | 1989 | 2530155 |
| evidence of paf-acether metabolic pathway activation in antigen challenge of upper respiratory airways. | lyso-paf-acether and paf-acether (formerly platelet-activating factor) were detected in nasal secretions from patients with hay fever who underwent local antigen challenge. lyso-paf release was observed in 12 of 13 patients, with a maximum (p less than 0.001) 5 min after stimulation and a progressive decrease during the first hour. paf was detected in the 5-min postchallenge nasal washings from two of 13 subjects. after hplc, this mediator was found in four of seven postchallenge nasal washings ... | 1989 | 2546468 |
| the inhibitory actions of azelastine hydrochloride on the early and late bronchoconstrictor responses to inhaled allergen in atopic asthma. | we have examined the effect of azelastine hydrochloride, 8.8 mg, on the early and late responses to inhaled allergen in a group of 12 atopic subjects with asthma. on two separate days, 3 weeks apart, patients were administered either oral azelastine, 8.8 mg, or matched placebo. four hours later they inhaled via nebulizer, a dose of allergen (grass pollen or dermatophagoides pteronyssinus) that had previously been demonstrated to produce a 25% fall in fev1. plasma-histamine concentrations and fev ... | 1989 | 2572616 |
| monoclonal anti-human igg antibodies for quantitation of allergen-specific igg in human sera. | eight monoclonal anti-human igg antibodies were fully characterized and evaluated as possible reagents in solid phase radioimmunoassay for quantitating allergen-specific igg antibody. four monoclonal antibodies (hg24d, hg2-14, hg2-18, and hg2-25) recognize ch2 domain of human igg and bind to human igg fixed to microtiter plate with high affinities. these monoclonal antibodies were more suitable than polyclonal rabbit anti-human igg antibody in phadebas rast for honey bee venom-specific igg antib ... | 1985 | 2578496 |
| leukocyte histamine release to suxamethonium in patients with adverse reactions to muscle relaxants. | in an earlier study we confirmed the usefulness of intradermal skin tests and histamine release in diagnosis of patients reactive to muscle relaxants, and we suggested an ige-mediated reaction rather than an idiosyncratic mechanism. in a later study, we studied the relationship between (formula: see text) that is one of the muscle relaxants producing the most frequent adverse reactions under anesthesia. histamine release was measured in five patients with increasing concentrations of suxamethoni ... | 1985 | 2579116 |
| measurement of allergens associated with dust mite allergy. i. development of sensitive radioimmunoassays for the two groups of dermatophagoides mite allergens, der i and der ii. | sensitive radioimmunoassays were developed to measure the two groups of major allergens (der i and der ii) of dermatophagoides pteronyssinus and dermatophagoides farinae, in which radiolabeled protein a served as a general tracer. glass rods covalently coupled with f(ab')2 fragments of rabbit antiserum igg were incubated first with mite or house dust extracts, and then with affinity-purified rabbit antibodies. the bound allergen-antibody complex was detected with a 125i-labeled protein a. the tw ... | 1989 | 2583856 |
| investigation of the allergenic activity of isolated fractions of a standardized partly purified whole mite body (dermatophagoides pteronyssinus) extract. | twelve fractions of a molecular weight range of 1.35-670.00 kilodaltons (kd) were isolated from a biologically standardized partly purified whole mite body extract (dermatophagoides pteronyssinus) by preparative size exclusion high-performance liquid chromatography. the allergenic activity and the antigen and allergen patterns of the isolated fractions were investigated by rast, rast inhibition and crossed (radio)immunoelectrophoresis (cie/crie). by crie, each of the fractions showed allergen pa ... | 1989 | 2592112 |
| the value of total ige determination in mite allergy. | our aim was to study the behaviour of patients with perennial respiratory allergy in relation to total ige production. we chose patients allergic to house dust mite dermatophagoides pteronyssinus, because this is the most representative perennial antigen. the total ige in a wide group of patients was evaluated in relation to diagnosis, clinical activity, and seasonal changes. we also studied the possible changes in relation to immunotherapy. the total ige was measured through enzyme immunoassay ... | 2008 | 2610185 |
| [specific immunotherapy using the slow alpha fraction of dermatophagoides pteronyssinus. efficacy; tolerance]. | this open study was made on 126 patients (72 men, 54 women). specific immunotherapy (it) commenced in 1988 with the dermatophagoides pteronyssinus alpha fraction (alpha -f dpt) a new standardised, purified allergen, of better quality, but more reactive. the patient were aged between 4 and 66 years (mean 23.1 years) the subjects were young and the majority were male. most (93.6%) showed at least respiratory symptoms (asthma, rhinitis). in about a quarter of the patients there were also dermatolog ... | 1989 | 2624679 |
| [reactivity of the skin to the most common allergens in children aged 0-3 years with obstructive bronchitis]. | prick tests were carried out in 84 children aged 4-34 months with obstructive chronic bronchitis. three children reacted to negative control. all tested children reacted with a blister to positive control (0.1% histamine solution, 5% solution of codeine). positive prick tests were found in 33 children (40.7%). most often the children reacted to egg (16%), feathers and house mite--dermatophagoides pteronyssinus (13.6%), dust and cereals allergens (11.1%). in all positive reactive children elevate ... | 0 | 2628952 |
| [skin reactivity to grass pollen and the mite dermatophagoides pteronyssinus in the prick-test using 2 types of allergen preparations]. | the reliability of prick testing with allergen preparations produced in yugoslavia was assessed by comparison to the internationally accepted ones (pharmacia diagnostica) of 100,000 bu activity. the following allergens were used: the mite dermatophagoides pteronyssinus in 107 subjects, and grass pollens: dactylis glomerata, poa pratensis, phleum pratense and secale cereale in 39 subjects. the clinical significance of the response was assessed in relation to the skin reaction to the negative cont ... | 1989 | 2638564 |