Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| immunolocalization of allatostatin-like neuropeptides and their putative receptor in eyestalks of the tiger prawn, penaeus monodon. | allatostatin (ast)-like immunoreactivity (ir) was localized in the eyestalk of penaeus monodon by immunohistochemistry using four anti-ast antibodies. depending on the antisera, ast-like immunoreactivity was detected in neuronal bodies of the lamina ganglionalis, cell bodies anterior to the medulla externa and cell bodies on the anterior and posterior of the medulla terminalis. neuronal processes in neuropiles of the medulla externa, medulla terminalis, sinus gland and nerve fibers in the optic ... | 2003 | 14706535 |
| abundance of potentially pathogenic micro-organisms in penaeus monodon larvae rearing systems in india. | monodon baculovirls (mbv), external fouling organisms (efo) and bacteria (especially vibrio species) were monitored during 1996-1997 at nine different penaeus monodon rearing hatcheries in india. total cultivable heterotrophic bacteria, vibrio-like-bacteria, presumptive vibrio harveyi, vibrio anguillarum, vibrio vulnificus counts were determined from shrimp eggs, post larvae, rearing tank water, source sea water, feed (artemia nauplii and microencapsulated feed). the mbv infected post larvae and ... | 2003 | 14717450 |
| expression of biologically active crustacean hyperglycemic hormone (chh) of penaeus monodon in pichia pastoris. | crustacean hyperglycemic hormone (chh), molt-inhibiting hormone (mih), and gonad-inhibiting hormone (gih) are members of a major peptide family produced from the x-organ sinus gland complex in the eyestalk of crustaceans. this peptide family plays important roles in controlling several physiologic processes such as regulation of growth and reproduction. in this study the complementary dna encoding a peptide related to the chh/mih/gih family (so-called pem-cmg) of the black tiger prawn penaeus mo ... | 2003 | 14719165 |
| identification of gyrkppfngsifamide (crustacean-sifamide) in the crayfish procambarus clarkii by topological mass spectrometry analysis. | a new concept relating to the purification protocol for biological proteins and peptides has been designed as "topological mass spectrometry analysis," in combination with maldi-tof ms using slices of tissues, chromatographic purification from the extract of tissues, molecular cloning for the determination of the precursor structure, and capillary lc-ms/ms analysis for elucidation of its posttranslational modifications. in an actual application, we identified an alpha-amidated neuropeptide from ... | 2004 | 14723891 |
| occurrence, histopathology and experimental transmission of hepatopancreatic parvovirus infection in penaeus monodon postlarvae. | hepatopancreatic parvovirus (hpv) was detected in samples of penaeus monodon post-larvae (pl-13, pl-18, pl-19, pl-26) from 2 hatcheries in 2 provinces (samar and iloilo) in the philippines. the percentage of infection was 20 to 100% in postlarvae obtained from the hatchery in samar in august 2001. postlarvae from the hatchery in iloilo, sampled in october and november 2001, had 70 to 99% hpv infection. wet mounts of squashed hepatopancreatic tissue stained with malachite green (wet-mount techniq ... | 2003 | 14735916 |
| detection by pcr of hepatopancreatic parvovirus (hpv) and other viruses in hatchery-reared penaeus monodon postlarvae. | the prevalence of hepatopancreatic parvovirus (hpv), monodon baculovirus (mbv) and white spot syndrome virus (wssv) in samples of penaeus monodon postlarvae (pl10 to pl20, 10 to 20 d old postlarvae) in india was studied by pcr. samples collected from different hatcheries, and also samples submitted by farmers from different coastal states, were analyzed. hpv was detected in 34%) of the hatchery samples and 31% of the samples submitted by farmers, using a primer set designed for detection of hpv ... | 2003 | 14735932 |
| vibrio alginolyticus associated with white spot disease of penaeus monodon. | in february 2000, white spot disease outbreaks occurred among cultured penaeus monodon in extensive shrimp farms on the southwest coast of india. bacteria were isolated from infected shrimp that showed reddish body coloration and white spots in the cuticle. the isolates were screened on thiosulfate citrate bile salt sucrose (tcbs) agar plates for the selection of vibrio species. the primary isolate (qs7) was characterized as v. alginolyticus based on morphological, biochemical and physiological ... | 2003 | 14735933 |
| a single wap domain-containing protein from litopenaeus vannamei hemocytes. | a cdna clone coding for a single wap domain (swd) protein was isolated from a hemocyte cdna library of litopenaeus vannamei. the full-length cdna sequence is 0.4kb long and encodes a 93-amino acid protein. using this sequence as a probe a similar clone coding for a 92-amino acids protein was found in a cdna library from penaeus monodon hemocytes. the mrna size was confirmed by northern blot as well as that gene is expressed in hemocytes, but not in hepatopancreas. mrna levels of the shrimp swd p ... | 2004 | 14741689 |
| protection of penaeus monodon against white spot syndrome virus by oral vaccination. | white spot syndrome virus (wssv) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. no adequate treatments against this virus are available. it is generally accepted that invertebrates such as shrimp do not have an adaptive immune response system such as that present in vertebrates. as it has been demonstrated that shrimp surviving a wssv infection have higher survival rates upon subsequent rechallenge, we investigated the potential of ora ... | 2004 | 14747570 |
| detection and differentiation of yellow head complex viruses using monoclonal antibodies. | three monoclonal antibodies (mabs) raised against pathogenic yellow head virus (yhv) from thailand were tested against tissues of shrimp from thailand, australia, ecuador and india that were purported to be infected with yellow head complex viruses. mabs v-3-2b and y-18 were specific to gp116 and gp64 envelope proteins, respectively, while y-19 was specific to a 20 kda putative nucleoprotein p20. as a preliminary step, the site of reactivity of the 3 mabs in yhv was determined by immuno-electron ... | 2003 | 14960031 |
| identification of immune-related genes in hemocytes of black tiger shrimp (penaeus monodon). | an expressed sequence tag (est) library was constructed from hemocytes of the black tiger shrimp (penaeus monodon) to identify genes associated with immunity in this economically important species. the number of complementary dna clones in the constructed library was approximately 4 x 10(5). of these, 615 clones having inserts larger than 500 bp were unidirectionally sequenced and analyzed by homology searches against data in genbank. significant homology to known genes was found in 314 (51%) of ... | 2002 | 14961242 |
| identification of two distinct molt-inhibiting hormone-related peptides from the giant tiger prawn penaeus monodon. | six peptides belonging to the crustacean hyperglycemic hormone (chh) family were isolated from extracts of the sinus glands of the giant tiger prawn penaeus monodon by reverse-phase high-performance liquid chromatography. these were designated pem-sgp-a to pem-sga-f (pem, penaeus monodon; sgp, sinus gland peptide) and their amino-terminal amino acid sequences were analyzed. five of the 6 peptides (pem-sgp-a, -b, -d, -e, and -f) exhibited similar amino acid sequences to those of chh peptides that ... | 2002 | 14961272 |
| development of vitellogenin-elisa, an in vivo bioassay, and identification of two vitellogenesis-inhibiting hormones of the tiger shrimp penaeus monodon. | a sandwich enzyme-linked immunosorbent assay (elisa) for the quantification of vitellogenin in the hemolymph of penaeus monodon is reported. lipovitellin from the mature ovary was purified using hydroxylapatite column chromatography and used as the standard protein, which was serially diluted from 3336 to 6.51 microg and gave a linear plot. sensitivity results showed elisa was insensitive to samples that did not contain vitellogenin or lipovitellin. specificity results showed the degree of the d ... | 2001 | 14961329 |
| genetic heterogeneity of the giant tiger shrimp (penaeus monodon) in thailand revealed by rapd and mitochondrial dna rflp analyses. | genetic diversity of the giant tiger shrimp (penaeus monodon) collected from 5 areas, chumphon and trat (gulf of thailand), and phangnga, satun, and trang (andaman sea), was examined by randomly amplified polymorphic dna (rapd) and mitochondrial dna (16s ribosomal dna and an intergenic coi-coii) polymorphism. a total of 53 polymorphic fragments from ubc299, ubc273, and ubc268 was consistently scored across all samples. from the respective primers 26, 32, and 30 genotypes were generated. a 260-bp ... | 2001 | 14961335 |
| a synthetic antibacterial peptide from mytilus galloprovincialis reduces mortality due to white spot syndrome virus in palaemonid shrimp. | white spot syndrome virus (wssv) isolated from penaeus monodon was found to be highly infective for the western mediterranean shrimp, palaemon sp. using polymerase chain reaction (pcr), it was demonstrated that such shrimp are not naturally carriers of wssv. following challenge with virus, mortality reached 100% 3.5-4 days after injection at 22 degrees c. incubation of infected shrimp at 10 degrees c totally suppressed the mortality which rapidly developed when shrimp were returned to 18 or 22 d ... | 2004 | 14986940 |
| molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (tctp) gene from penaeus monodon shrimp. | white spot syndrome, caused by white spot syndrome virus (wssv), is a deadly disease of shrimps, causing a catastrophic loss in shrimp industries worldwide. in order to investigate molecular response of shrimp haemocyte to wssv infection, we performed subtraction hybridization of mrnas from healthy and wssv-infected haemocyte. one of the genes that were severely down-regulated in moribund wssv-infected-haemocyte was translationally controlled tumor protein (tctp) (or fortilin). strikingly, while ... | 2004 | 15006423 |
| multiplex rt-nested pcr differentiation of gill-associated virus (australia) from yellow head virus (thailand) of penaeus monodon. | a multiplex rt-nested pcr has been developed to detect and differentiate the closely related prawn viruses, gill-associated virus (gav) from australia and yellow head virus (yhv) from thailand. rt-pcr using primers to conserved sequences in the orf1b gene amplified a 794 bp region of either gav or yhv. nested pcr using a conserved sense primer and either a gav- or yhv-specific antisense primer to a divergent sequence differentially amplified a 277 bp region of the primary pcr amplicon. multiplex ... | 2004 | 15019259 |
| structural properties, conformational stability and oxygen binding properties of penaeus monodon hemocyanin. | hemocyanin sequences allineament shows the presence of highly invariant regions especially in the active site and in the tight intersubunits interaction sites. comparing the aminoacids in contact regions between monomers is possible to interpret the stability of hexamers. | 2004 | 15036290 |
| microsporidians in penaeid shrimp along the west coast of madagascar. | three species of penaeid shrimp, fenneropenaeus indicus, penaeus monodon and p. semisulcatus, found in trawler catches off the west coast of madagascar were infected with microsporidian parasites. the infections were evident as muscular lesions with a cottony appearance when abundant. spore size (2.6 x 1.6 microm) and morphology (ovoid) for the parasites infecting both f. indicus and p. semisulcatus were not significantly different, suggesting that they might be the same microsporidian species. ... | 2004 | 15038455 |
| microsatellite dna markers: evaluating their potential for estimating the proportion of hatchery-reared offspring in a stock enhancement programme. | we describe a statistical method for estimating the effectiveness of a stock enhancement programme using nuclear dna loci. it is based on knowing the population allele frequencies and the genotypes of the hatchery parents (mother only, or mother and father), and on determining the probability that a wild-born animal will by chance have a genotype consistent with hatchery origin. we show how to estimate the proportion of released animals in the wild population, and its standard error. the method ... | 2004 | 15078464 |
| detection of white spot syndrome virus and yellowhead virus in prawns imported into australia. | to determine whether viable white spot syndrome virus (wssv) or yellowhead virus (yhv) were present in prawn products imported into australia. | 2004 | 15088963 |
| rt-pcr detection of yellow head virus (yhv) infection in penaeus monodon using dried haemolymph spots. | sample collection and rna isolation from shrimp haemolymph for rt-pcr diagnosis of yellow head virus (yhv) infections is crucial for disease control programs for cultivated shrimp in thailand. problems with rna degradation arise when field samples must be collected far from the laboratory by relatively inexperienced personnel who do not have ready access to sophisticated reagents. in an attempt to solve this problem, haemolymph samples from shrimp were collected either by mixing with 10% (w/v) s ... | 2004 | 15109813 |
| protection of penaeus monodon against white spot syndrome virus using a wssv subunit vaccine. | although invertebrates lack a true adaptive immune response, the potential to vaccinate penaeus monodon shrimp against white spot syndrome virus (wssv) using the wssv envelope proteins vp19 and vp28 was evaluated. both structural wssv proteins were n-terminally fused to the maltose binding protein (mbp) and purified after expression in bacteria. shrimp were vaccinated by intramuscular injection of the purified wssv proteins and challenged 2 and 25 days after vaccination to assess the onset and d ... | 2004 | 15110331 |
| cdna sequence encoding an 11.5-kda antibacterial peptide of the shrimp penaeus monodon. | 2004 | 15110339 | |
| molecular cloning and sequencing of shrimp (penaeus monodon) penaeidin-5 cdna. | 2004 | 15110340 | |
| vitamin e requirements of juvenile grass shrimp, penaeus monodon, and effects on non-specific immune responses. | a feeding trial was conducted to determine the dietary vitamin e (dl-alpha-tocopheryl acetate, dl-alpha-toa) requirement and its effect on the non-specific immune responses of juvenile grass shrimp, penaeus monodon. purified diets with eight levels (0, 25, 50, 75, 100, 150, 200, 400 mg vitamin e kg diet-1) of supplemental dl-alpha-toa were fed to p. monodon (mean initial weight 0.29 +/- 0.01 g) for eight weeks. each diet was fed to three replicate groups of shrimp. weight gains and total haemocy ... | 2004 | 15123290 |
| molecular cloning and characterisation of a cell adhesion molecule, peroxinectin from the white shrimp litopenaeus vannamei. | expression of peroxinectin cdna was obtained from haemocytes of white shrimp litopenaeus vannamei using oligonucleotide primers based on the peroxinectin sequence of tiger shrimp penaeus monodon and freshwater crayfish pacifastacus leniusculus. the peroxinectin of l. vannamei was constitutively expressed. the cloning of peroxinectin cdna fragment was subjected to rt-pcr using degenerated primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nu ... | 2004 | 15145414 |
| evaluation of pseudomonas sp. pm 11 and vibrio fluvialis pm 17 on immune indices of tiger shrimp, penaeus monodon. | occurrence of widespread epizootics among cultured stock of shrimp has put research programmes on preventive approaches such as application of probiotics on a high priority in aquaculture. in the present study two bacteria, pseudomonas sp. pm 11 and vibrio fluvialis pm 17 were selected as candidate probionts from a pool of bacteria isolated from gut of farm reared sub-adult shrimp and tested for their effect on the immunity indicators of tiger shrimp. sub-adult shrimp, weighing 14 to 22 g were t ... | 2004 | 15212731 |
| susceptibility of the australian freshwater crayfish cherax destructor albidus to white spot syndrome virus (wssv). | cherax destructor occurs naturally and/or is farmed in all australian mainland states and territories and is of major cultural, economical and conservation significance. the aim of this study was to determine susceptibility of the commercially important subspecies c. destructor albidus to white spot syndrome virus (wssv), a hazard to crustaceans and currently considered to be exotic to australia. in challenge tests by intramuscular injection, c. destructor albidus displayed a similar level of su ... | 2004 | 15264714 |
| quantitative real-time rt-pcr demonstrates that handling stress can lead to rapid increases of gill-associated virus (gav) infection levels in penaeus monodon. | gill-associated virus (gav) of the black tiger prawn penaeus monodon has been implicated as a cause of periodic production losses in australia since 1996. we report here the development of a real-time quantitative rt-pcr (qrt-pcr) for gav. a dilution series of in vitro transcribed rna was used to determine the sensitivity limit of the qrt-pcr and as a standard for gav quantification. a linear relationship between cycle threshold (ct) values and input rna was obtained over a wide concentration ra ... | 2004 | 15264715 |
| evaluation of attractants and egg-laying substrate preference for oviposition by aedes albopictus (diptera: culicidae). | evaluation of oviposition attractants and substrate preferences of aedes albopictus was carried out under laboratory and field conditions. to obtain candidate oviposition substances we used a water rinse of 3 mollusk species: blood cockle (anadara granosa), carpet shell (paphia undulata), and sea mussel (mytilus smaragdinus), and the giant tiger prawn (penaeus monodon). the rinse water of carpet shell and giant tiger prawn showed higher attractiveness for ovipostion than the other candidate attr ... | 2004 | 15266742 |
| evaluation of dietary essentiality of vitamins for penaeus monodon (fabricius). | the effect of exclusion of individual water-soluble (thiamine, riboflavin, pyridoxine, cyanocobalamin, pantothenic acid, folic acid, niacin, biotin, choline, inositol, ascorbic acid) and fat-soluble vitamins (vit. a, d, k and e) in semi-purified diets on growth and survival of juvenile shrimp, p. monodon was studied in the laboratory for 8 weeks. diets lacking riboflavin and vitamin k did not affect growth and survival of shrimp. however, deletion of inositol and choline resulted in poor growth. ... | 2004 | 15274482 |
| the gene encoding the nucleocapsid protein of gill-associated nidovirus of penaeus monodon prawns is located upstream of the glycoprotein gene. | the orf2 gene of gill-associated virus (gav) of penaeus monodon prawns resides 93 nucleotides downstream of the orf1a-orf1b gene and encodes a 144-amino-acid hydrophilic polypeptide (15,998 da; pi, 9.75) containing 20 basic (14%) and 13 acidic (9%) residues and 19 prolines (13%). antiserum to a synthetic orf2 peptide or an escherichia coli-expressed glutathione s-transferase-orf2 fusion protein detected a 20-kda protein in infected lymphoid organ and gill tissues in western blots. the gav orf2 f ... | 2004 | 15280504 |
| occurrence of vibrio vulnificus in fish and shellfish available from markets in china. | vibrio vulnificus is a naturally occurring estuarine bacterium often associated with disease such as septicemia in humans following consumption of raw and lightly cooked seafood. in china and neighboring countries, rapid economic growth has encouraged increased consumption of seafood, and dietary habits are changing, with more people eating raw fish. in this study, the prevalence of v. vulnificus was investigated in 48 samples from 11 species of live seafood available from markets in coastal cit ... | 2004 | 15330524 |
| molecular modification of penaeus monodon sperm in female thelycum and its consequent responses. | using penaeus monodon as the model, we demonstrated the molecular changes and the mechanism of thelycal-dependent sperm modification resulting in an enhanced acrosome reaction (ar) response. attention was paid to the modification of the sperm plasma membrane which was mediated through an adsorption or removal of sperm peripheral and integral membrane proteins as indicated by the different profiles of these proteins in spermatophore (s) and thelycal (t) sperm. in vitro adsorption of alexa-488 con ... | 2004 | 15349848 |
| fusarium incarnatum isolated from black tiger shrimp, penaeus monodon fabricius, with black gill disease cultured in vietnam. | fusarium incarnatum was isolated from gill lesions of cultured black tiger shrimp, penaeus monodon, in every crop during 2000-2002 in nghe an province, vietnam. infected shrimps showed typical signs of black gill disease and mortalities about a month prior to harvest. detailed morphological examinations, as well as molecular phylogenic analyses based on partial nucleotide sequences of ribosomal dna, were made on the isolates. an artificial infection of kuruma prawn, penaeus japonicus, using two ... | 2004 | 15357709 |
| production of polyclonal antiserum against recombinant vp28 protein and its application for the detection of white spot syndrome virus in crustaceans. | the vp28 gene of white spot syndrome virus (wssv) was cloned into prset b expression vector. the vp28 protein was expressed as a protein with a 6-histidine taq in escherichia coli gj1158 with nacl induction. antiserum was raised against this recombinant-vp28 protein in rabbits and it recognized vp28 protein in naturally and experimentally wssv-infected shrimp, marine crabs, freshwater prawns and freshwater crabs. the antiserum did not recognize any of the other known wssv structural proteins. va ... | 2004 | 15357710 |
| genetic and phenotypic variations of isolates of shrimp taura syndrome virus found in penaeus monodon and metapenaeus ensis in taiwan. | distinct taura syndrome virus (tsv) isolates were found in metapenaeus ensis (isolate tw2kmetsv), penaeus monodon (isolate tw2kpmtsv) and litopenaeus vannamei (isolate tw02lvtsv). nucleotide sequence analysis of these three isolates revealed differences in the tsv structural protein (capsid protein precursor) gene orf2. tsv orf2 amino acid sequence comparison and phylogenetic analysis suggested a comparatively close relationship between these three taiwanese isolates and the hawaiian isolate hi9 ... | 2004 | 15448359 |
| multiple pathogens found in growth-retarded black tiger shrimp penaeus monodon cultivated in thailand. | in 2001-2002 throughout thailand, black tiger shrimp penaeus monodon farmers reported very unusual retarded growth. we have called this problem monodon slow growth syndrome (msgs). based on decreased national production, estimated losses due to this phenomenon were in the range of 13 000 million baht (approximately 300 million us dollars) in 2002. since rearing practices had not changed, it was considered possible that the msgs problem may have arisen from a new or existing pathogen. to examine ... | 2004 | 15460852 |
| molecular structure and organization of crustacean hyperglycemic hormone genes of penaeus monodon. | the crustacean hyperglycemic hormone (chh) has been shown to exist as multiple molecular forms in several crustacean species. in penaeus monodon, a gene encoding chh (so-called pem-chh1) was recently described. in this study, the molecular structures of two other chh genes (pem-chh2 and pem-chh3) are reported. both the pem-chh2 and pem-chh3 genes contain three exons that are separated by two introns that are similar to the structure of other genes in the same family. an analysis of the upstream ... | 2004 | 15469693 |
| characterisation of a serine proteinase from penaeus vannamei haemocytes. | serine proteinases are involved, besides digestive role, in immune response processes. in addition to the typical serine proteinase domain, proteinases from arthropod haemocytes contain so-called clip domains which are believed to exert regulatory functions. clones coding for clip domain-containing serine proteinases were isolated from both penaeus vannamei and penaeus monodon haemocyte cdna libraries. these proteins have most of the structural characteristics of serine proteinase domain, but in ... | 2005 | 15475307 |
| cdna sequence encoding an antimicrobial peptide of chelonianin from the tiger shrimp penaeus monodon. | 2005 | 15475313 | |
| monoclonal antibodies specific to haemocytes of black tiger prawn penaeus monodon. | monoclonal antibodies specific to haemocytes of penaeus monodon were generated from a mouse immunized with a mixture of sds-treated and formalin-fixed haemocytes. hybridoma clones were selected by immunohistochemistry against fixed haemocytes, heart, lymphoid organ, and haemopoietic tissue, and western blot against haemocyte extract and haemolymph. sixteen monoclonal antibodies specific to haemocytes were obtained and could be divided into six groups according to their binding capacities to vari ... | 2005 | 15519539 |
| molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) from the white shrimp litopenaeus vannamei. | a lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte and hepatopancreas of white shrimp litopenaeus vannamei using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nucleotide sequence revealed that the cdna clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. the calculated molecular mass of the m ... | 2005 | 15561560 |
| antimicrobial peptides discovered in the black tiger shrimp penaeus monodon using the est approach. | two cdna libraries were prepared from hemocytes of normal and vibrio harveyi-challenged black tiger shrimp penaeus monodon. a total of 1062 expressed sequence tag (est) clones were sequenced unidirectionally. ests representing the antimicrobial peptide (amp) homologues, antilipopolysaccharide factors (alf), penaeidins and crustins were discovered. they predominated among immune-related genes, representing 29.2% and 64.0% of the normal and challenged libraries, respectively. several types of each ... | 2004 | 15584419 |
| rediscovery of the australian strain of infectious hypodermal and haematopoietic necrosis virus. | in this study we rediscovered and verified the presence of an infectious hypodermal and haematopoietic necrosis virus (ihhnv) strain amongst cultured penaeid prawns penaeus monodon from 1993 in australia on the basis of a pcr analysis with ihhnv specific primers and sequencing of the resulting amplicons. a total of 7 previously published diagnostic primers specific to ihhnv were tested against australian penaeid prawns and only 1 elicited a positive ihhnv pcr result with 16 out of 20 australian ... | 2004 | 15584422 |
| cloning and molecular characterization of heat shock cognate 70 from tiger shrimp (penaeus monodon). | we cloned the complementary deoxyribonucleic acid (cdna) of the heat shock cognate 70 (hsc70) gene of tiger shrimp (penaeus monodon). it was 2207 bp long and included a 1959-bp coding region, a 40-bp flanking region at the 5' end, and a 208-bp flanking region at the 3' end. the deduced, 652-amino acid sequence had a molecular mass of 71 481 da and an estimated isoelectric point (pi) of 5.2. based on phylogenetic analysis, the gene is clustered with the hsc70 proteins of invertebrates and vertebr ... | 2004 | 15633291 |
| interaction of ph with mercuric chloride toxicity to penaeid prawns from a tropical estuary, east coast of india: enhanced toxicity at low ph. | toxicity tests were conducted to study the interaction of ph and the response of two size groups of penaeid prawns i.e. penaeus monodon and penaeus indicus to different sets of five concentrations of hgcl2 (0.01-0.09 mg l(-1)) under a broad range of ph conditions (5-9). behavioural responses varied according to test solution concentration and nominated values of ph. abnormality was detected in the higher concentrations with lower ph in smaller size group of p. monodon. high mortality was observe ... | 2005 | 15667843 |
| molecular cloning and characterisation of prophenoloxidase from haemocytes of the white shrimp, litopenaeus vannamei. | cdna encoding prophenoloxidase (propo) of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna using oligonucleotide primers based on the propo sequence of tiger shrimp penaeus monodon, freshwater crayfish pacifastacus leniusculus, green tiger shrimp penaeus semisulcatus (accession no.: af521949) and kuruma shrimp marsupenaeus japonicus (accession no.: ab0733223). propo of l. vannamei was ... | 2005 | 15683918 |
| cyanobacteria and prawn farming in northern new south wales, australia--a case study on cyanobacteria diversity and hepatotoxin bioaccumulation. | harmful cyanobacteria pose a hazard to aquatic ecosystems due to toxins (hepatotoxic microcystins, nodularins, and cylindrospermopsin) they produce. the microcystins and nodularins are potent toxins, which are also tumor promoters. the microcystins and nodularins may accumulate into aquatic organisms and be transferred to higher trophic levels, and eventually affect vector animals and consumers. prawn farming is a rapidly growing industry in australia. because information regarding effects of cy ... | 2005 | 15737678 |
| response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus. | white spot syndrome virus (wssv) is a serious pathogen of aquatic crustaceans. little is known about its transmission in vivo and the immune reaction of its hosts. in this study, the circulating haemocytes of crayfish, procambarus clarkii, infected by wssv, and primary haemocyte cultures inoculated with wssv, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. in ultra-thin sections of infected haemocytes, the enveloped virions we ... | 2005 | 15752275 |
| recombinant expression and characterization of five-domain kazal-type serine proteinase inhibitor of black tiger shrimp (penaeus monodon). | four full-length complementary dnas of kazal-type serine proteinase inhibitors (spis) were identified from hemocyte cdna libraries of the black tiger shrimp penaeus monodon and recognized as having 4 (spipm1) and 5 (spipm2, spipm3, and spipm4) kazal domains. spipm2 encoding a complete 5-kazal-domain inhibitor was expressed in the escherichia coli system. inhibitory activity of crude proteins against various serine proteases was tested using spi activity gelatin with sodium dodecylsulfate polyacr ... | 2005 | 15756478 |
| antiserum to the gp116 glycoprotein of yellow head virus neutralizes infectivity in primary lymphoid organ cells of penaeus monodon. | yellow head virus (yhv) is an invertebrate nidovirus that has caused mass mortality of cultured penaeus monodon in asia. in this study, we investigated whether mouse polyclonal antiserum raised against the yhv gp116 or gp64 structural glycoproteins could neutralize yhv infectivity as determined using an in vitro quantal assay in primary cultures of lymphoid organ cells. anti-gp116 antiserum showed virus-neutralizing activity whereas anti-gp64 antiserum failed to inhibit infection. the results su ... | 2005 | 15759804 |
| bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. | new methods were developed to assess immunostimulant efficacy in the black tiger shrimp penaeus monodon. test shrimp were fed with 2 or 4 % yeast extract (ye)-coated feed while controls were fed non-coated feed. after 4 wk of feeding, individual shrimp were assessed for total hemocyte counts (thc), the number of granular hemocytes (gh) and rate of bacterial clearance. for hemocyte counts, formalin-fixed hemolymph was stained with 1.2 % rose bengal in 50 % ethanol for 20 min at room temperature. ... | 2005 | 15759805 |
| molecular cloning and characterization of cdna of penaeidin-like antimicrobial peptide from tiger shrimp (penaeus monodon). | we report the molecular cloning and characterization of a penaeidin-like antimicrobial peptide (amp) complementary dna from the hemocytes of tiger shrimp (penaeus monodoni). a tiger shrimp amp cdna containing the entire coding region of the peptide, determined by rapid amplification of cdna 5' and 3' ends and polymerase chain reaction of the messenger rna isolated from hemocytes of the shrimp, is 683 bp in length with an open reading frame of 222 bp. the deduced amino acid sequence of this antim ... | 2005 | 15776314 |
| polychaete worms--a vector for white spot syndrome virus (wssv). | the present work provides the first evidence of polychaete worms as passive vectors of white spot syndrome virus (wssv) in the transmission of white spot disease to penaeus monodon broodstocks. the study was based on live polychaete worms, marphysa spp., obtained from worm suppliers/worm fishers as well as samples collected from 8 stations on the northern coast of tamilnadu (india). tiger shrimp penaeus monodon broodstock with undeveloped ovaries were experimentally infected with wssv by feeding ... | 2005 | 15819425 |
| production of monoclonal antibodies for detection of vibrio harveyi. | monoclonal antibodies (mabs) against vibrio harveyi were produced from mice immunized with heat-killed and sds-mercaptoethanol-treated highly virulent v. harveyi 639. fifteen mabs were selected and sorted into 6 groups according to their specificity to various proteins of apparent molecular weight ranging from 8 to 49 kda. some antibodies were used for detection of v. harveyi at concentrations as low as 10(4) cfu ml(-1) using immunodot blots. most of the selected mabs did not show cross-reactivi ... | 2005 | 15819431 |
| detection of hepatopancreatic parvovirus (hpv) in wild shrimp from india by nested polymerase chain reaction (pcr). | the prevalence of hepatopancreatic parvovirus (hpv) in wild penaeid shrimp samples from india was studied by nested polymerase chain reaction (pcr) using primers designed in our laboratory. the virus could be detected in 9 out of 119 samples by non-nested pcr. however, by nested pcr 69 out of 119 samples were positive. the pcr results were confirmed by hybridization with digoxigenin-labelled dna probe. shrimp species positive by non-nested pcr included penaeus monodon, penaeus indicus and penaeu ... | 2005 | 15819441 |
| in situ hybridization demonstrates that litopenaeus vannamei, l. stylirostris and penaeus monodon are susceptible to experimental infection with infectious myonecrosis virus (imnv). | infectious myonecrosis virus (imnv) was recently found to be the cause of necrosis in the skeletal muscle of farm-reared litopenaeus vannamei from northeastern brazil. nucleic acid extracted from semi-purified imn virions showed that this virus contains a 7.5 kb rna genome. a cdna library was constructed, and a clone, designated as imnv-317, was labeled with digoxigenin-11-dutp and used as a gene probe for in situ hybridization (ish). this probe specifically detected imnv in infected tissues. to ... | 2005 | 15819442 |
| quaternary structure and functional properties of penaeus monodon hemocyanin. | the hemocyanin of the tiger shrimp, penaeus monodon, was investigated with respect to stability and oxygen binding. while hexamers occur as a major component, dodecamers and traces of higher aggregates are also found. both the hexamers and dodecamers were found to be extremely stable against dissociation at high ph, independently of the presence of calcium ions, in contrast to the known crustacean hemocyanins. this could be caused by only a few additional noncovalent interactions between amino a ... | 2005 | 15819896 |
| fitness and virulence of an ancestral white spot syndrome virus isolate from shrimp. | white spot syndrome virus, the type species of the virus family nimaviridae, is a large dsdna virus infecting shrimp and other crustaceans. genomic analysis of three completely sequenced wssv isolates identified two major polymorphic loci, "variable region orf14/15" and "variable region orf23/24". here, we characterize a wssv isolate originating from shrimp collected in thailand in 1996 (th-96-ii). this isolate contains the largest wssv genome ( approximately 312 kb) identified so far, mainly be ... | 2005 | 15845251 |
| protein expression in white spot syndrome virus infected penaeus monodon fabricius. | white spot syndrome virus (wssv) is the causative agent of the white spot disease of shrimp. penaeus monodon were captured from muttukadu estuary in chennai, india, transported to the laboratory and maintained in an aerated system with continuous water circulation-biofiltration. wssv-free p. monodon were challenged by feeding them only once with wssv-infected tissues of p. monodon. cumulative mortality (100%) of the infected individuals was determined. tissues from infected and uninfected shrimp ... | 2005 | 15845264 |
| an ancient role for a prokineticin domain in invertebrate hematopoiesis. | hemopoietic development requires firm control of cell proliferation and differentiation. although recent research has revealed conserved function of transcription factors and signaling pathways regulating lineage commitment in hemopoietic development in drosophila melanogaster and vertebrates, little is known about hemopoietic cytokines among the invertebrate phyla. in the present study, we show that differentiation and growth of hemopoietic stem cells in vitro from an invertebrate, pacifastacus ... | 2005 | 15879111 |
| activity and substrate specificity of cytosolic and microsomal glutathione s-transferase in australian black tiger prawns (penaeus monodon) after exposure to cyanobacterial toxins. | cyanobacterial toxins have been shown to have a far-reaching impact-from aquatic organisms to human health. aquatic organisms are typically exposed in their natural environment to toxic cyanobacteria, and exposure can occur via ingestion of cyanobacterial cells or by bioaccumulation of water-borne toxin. the aquaculture and fisheries of crustaceans are among the most important seafood industries. concomitant with the growth of this industry, the importance of the health of crustaceans increased. ... | 2005 | 15892065 |
| differences in susceptibility of palaemonid shrimp species to yellow head virus (yhv) infection. | five species of palaemonid shrimp, macrobrachium rosenbergii, m. lanchesteri, m. sintangense, palaemon styliferus and p. serrifer, were collected from penaeus monodon farming areas in thailand. some of each species were artificially infected with yellow head virus (yhv) by injection and then monitored by rt-pcr and by immunohistochemistry using monoclonal antibodies specific to 116 kda, 64 kda, and 20 kda proteins of yhv. natural yhv infections were not detected in any of the shrimp examined. in ... | 2005 | 15900682 |
| snp analysis of amy2 and ctsl genes in litopenaeus vannamei and penaeus monodon shrimp. | genetic studies in shrimp have focused on disease, with production traits such as growth left unexamined. two shrimp species, litopenaeus vannamei and penaeus monodon, which represent the majority of us shrimp imports, were selected for single nucleotide polymorphism (snp) discovery in alpha-amylase (amy2) and cathepsin-l (ctsl), both candidate genes for growth. in l. vannamei, four snps were found in amy2 and one snp was found in ctsl. in p. monodon, one snp was identified in ctsl. the ctsl gen ... | 2005 | 15932404 |
| gene-expression profiling of white spot syndrome virus in vivo. | white spot syndrome virus, type species of the genus whispovirus in the family nimaviridae, is a large, double-stranded dna (dsdna) virus that infects crustaceans. the genome of the completely sequenced isolate wssv-th encodes 184 putative open reading frames (orfs), the functions of which are largely unknown. to study the transcription of these orfs, a dna microarray was constructed, containing probes corresponding to nearly all putative wssv-th orfs. transcripts of 79 % of these orfs could be ... | 2005 | 15958687 |
| cloning the prophenoloxidase cdna and monitoring the expression of propo mrna in prawns (macrobrachium rosenbergii) stimulated in vivo by cpg oligodeoxynucleotides. | the prophenoloxidase (propo) system forms the basis of the non-specific defence system in crustaceans. the aim of this study was to develop an rt-pcr procedure to determine propo gene expression. we used several degenerate primers designed from the conserved regions in amino acid sequences of propos from other species to clone the possible cdna(s) from the haemocytes of the prawn macrobrachium rosenbergii. one dna fragment, 2016 bp long, was cloned by a combination of reverse transcriptase-polym ... | 2006 | 15961318 |
| recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor (alf) from the black tiger shrimp penaeus monodon. | anti-lipopolysaccharide factors (alfs), originally characterized from horseshoe crabs, have been recently identified from hemocytes of the black tiger shrimp, penaeus monodon, by a genomic approach. in order to characterize the properties and biological activities of this immune effector in shrimp, alfpm3, the most abundant isoform found in p. monodon, was expressed in the yeast pichia pastoris. large-scale production in fermentor provided 262 mg/l of recombinant alfpm3 which was purified to hom ... | 2005 | 15978281 |
| more than one type of transglutaminase in invertebrates? a second type of transglutaminase is involved in shrimp coagulation. | coagulation (clot formation) forms a physical barrier to prevent the loss of body fluid and dissemination of microbes into the haemocoel after injury or infection. its quickness and efficiency are essential for the survival of invertebrates that rely solely on innate immunity. transglutaminase (tg) catalyses intermolecular or intramolecular epsilon-(gamma-glutamyl) lysine bond formation, resulting in a protein polymerisation, and plays a role in blood coagulation and post-translational protein r ... | 2005 | 15985293 |
| partial characterization of a novel bacteriophage of vibrio harveyi isolated from shrimp culture ponds in thailand. | a bacteriophage was isolated together with vibrio harveyi (vh) 1114 a from a black tiger shrimp-rearing pond in thailand. by negative staining transmission electron microscopy (tem), the phage had an icosahedral head (diameter 60-62 nm), a rigid, non-contractile tail (9-10 nm x 100-120 nm) without a collar or terminal fibers and a genome of double stranded dna of approximately 80 kb as determined by analysis of restriction enzyme digestion fragments. since these features would place it in the fa ... | 2005 | 16023243 |
| molecular typing of vibrio parahaemolyticus strains isolated from the philippines by pcr-based methods. | the main aim of the present study was to use three pcr-based techniques for the analysis of genetic variability among vibrio parahaemolyticus strains isolated from the philippines. | 2005 | 16033470 |
| synthethic peptide used to develop antibodies for detection of polyhedrin from monodon baculovirus (mbv). | the use of previously published primers to amplify the monodon baculovirus (mbv) polyhedrin gene sequence by polymerase chain reaction (pcr) from post larvae (pl) of thai penaeus monodon resulted in failure. as a result, the putative polyhedrin protein of mbv was isolated from infected pl by homogenization, differential centrifugation and density gradient centrifugation with verification by transmission electron microscopy (tem). by sds-page, a single major protein band at 58 kda was obtained fr ... | 2005 | 16042047 |
| generation of monoclonal antibodies specific to hepatopancreatic parvovirus (hpv) from penaeus monodon. | hepatopancreatic parvovirus (hpv) was isolated from the hepatopancreas (hp) of slow growth penaeus monodon by urografin gradient centrifugation. the presence of hpv in the fraction was monitored by pcr and sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). only 1 major 54 kda protein band was observed in the strong pcr-positive fractions used to immunize mice for monoclonal antibody production. after cell fusion, the first step in selecting specific antibodies was performed b ... | 2005 | 16042048 |
| sirna injection induces sequence-independent protection in penaeus monodon against white spot syndrome virus. | white spot syndrome virus (wssv) is a major disease in crustaceans, particularly shrimp, due to the current intensity of aquaculture practices. novel strategies including vaccination to control this virus would be highly desirable. however, invertebrates lack a true adaptive immune response system and seem to rely on various innate immune responses. an alternative and more specific approach to counteract wssv infections in shrimp could be by the exploitation of rna interference. as long dsrna mo ... | 2005 | 16043253 |
| white spot syndrome virus isolates of tiger shrimp penaeus monodon (fabricious) in india are similar to exotic isolates as revealed by polymerase chain reaction and electron microscopy. | microbiological analysis of samples collected from cases of white spot disease outbreaks in cultured shrimp in different farms located in three regions along east coast of india viz. chidambram (tamil nadu), nellore (andhra pradesh) and balasore (orissa), revealed presence of vibrio alginolyticus, vibrio parahaemolyticus, and aeromonas spp. but experimental infection trials in penaeus monodon with these isolates did not induce any acute mortality or formation of white spots on carapace. infectio ... | 2005 | 16053274 |
| species identification of five penaeid shrimps using pcr-rflp and sscp analyses of 16s ribosomal dna. | dna-based molecular markers for differentiation of five penaeid shrimps (penaeus monodon, p. semisulcatus, feneropenaeus merguiensis, litopenaeus vannamei and marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and single-stranded conformation polymorphism (sscp) of 16s ribosomal (r) dna. differentiation of p. monodon, p. semisulcatus and l. vannamei can be unambiguously carried out by pcrrflp of 16s rdna(560) whereas p. s ... | 2005 | 16053718 |
| identification and characterization of syntenin binding protein in the black tiger shrimp penaeus monodon. | shrimp exhibit a diverse response to viral infection that is manifested in drastic up- and down-regulations of a variety of genes. in our previous work, we identified syntenin of the shrimp penaeus monodon (pm) as a dynamic responder to white spot syndrome virus (wssv) infection, its message being greatly upregulated in the acute phase of the infection. in order to further explore the link between pm-syntenin and viral infection, we performed a yeast two-hybrid screening of a p. monodon cdna lib ... | 2005 | 16055222 |
| in vitro susceptibility of antibiotics against vibrio spp. and aeromonas spp. isolated from penaeus monodon hatcheries and ponds. | susceptibility patterns to 16 different antibiotics were investigated against pathogenic vibrio spp. and aeromonas spp. isolated from shrimp culture hatcheries and ponds in india. thirteen species of vibrio (n = 90) and two species of aeromonas (n = 7) isolates were tested by agar disk diffusion. the results show that 100% of the isolates were resistant to ampicillin, and that 43.2% and 47.4% were sensitive to chlortetracycline and erythromycin, respectively. susceptibility patterns of another 1 ... | 2005 | 16139992 |
| physiological function and metabolism of free d-alanine in aquatic animals. | aquatic crustaceans and some bivalve mollusks contain a large amount of free d-alanine (up to 100 mumol/g wet wt.) in their tissues. under high salinity stress, crustaceans and bivalve mollusks largely accumulate d- and l-alanine irrespective of species examined, together with l-glutamine, l-proline, and glycine of which increases are species dependent. these data indicate that d-alanine is one of the major compatible osmolytes responsible for the intracellular isosmotic regulation in the tissue ... | 2005 | 16141518 |
| cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the european lobster, homarus gammarus, and its response to infection with bacteria. | degenerate pcr was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the european lobster, homarus gammarus. rapid amplification of cdna ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. the inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, carcinus maenas. the ... | 2006 | 16144710 |
| purification and partial characterization of the plasma clotting protein from the pink shrimp farfantepenaeus paulensis. | a clotting protein (cp) was purified from the plasma of the pink shrimp farfantepenaeus paulensis by sequential anion-exchange chromatography. the shrimp cp was able to form stable clots in vitro in the presence of hemocyte lysate and ca2+, suggesting that the clotting reaction is catalyzed by a ca2+-dependent transglutaminase present in shrimp hemocytes. dansylcadaverine was incorporated into the shrimp cp in the presence of endogenous transglutaminase (hemocyte lysate), confirming that the shr ... | 2005 | 16153866 |
| molecular cloning and characterisation of cytosolic manganese superoxide dismutase (cytmn-sod) from the giant freshwater prawn macrobrachium rosenbergii. | a cytosolic manganese superoxide dismutase (cytmn-sod) cdna was cloned from the hepatopancreas of giant freshwater prawn macrobrachium rosenbergii using reverse transcription polymerase chain reaction (rt-pcr) by degenerate primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nucleotide sequence revealed that the cytmn-sod cdna clone consists of 1339 bp with an open reading frame of 858 bp encoding a protein of 286 amino acids. the calculated ... | 2006 | 16154362 |
| effect of salinity on the immune response of tiger shrimp penaeus monodon and its susceptibility to photobacterium damselae subsp. damselae. | addition of nacl at 2.5% to tryptic soy broth (tsb) significantly increased the growth of photobacterium damselae subsp. damselae. tiger shrimp penaeus monodon held in 25 per thousand seawater were injected with p. damsela subsp. damselae grown in tsb containing nacl at 0.5%, 1.5%, 2.5% and 3.5% at a dose of 8.48 x 10(4)colony-forming units (cfu)shrimp(-1). over 24-96 h, the cumulative mortality was significantly higher for the shrimp challenged with p. damselae subsp. damselae grown in 2.5% nac ... | 2006 | 16213751 |
| rt-nested pcr detection of mourilyan virus in australian penaeus monodon and its tissue distribution in healthy and moribund prawns. | mourilyan virus (mov) is a newly identified virus of penaeus monodon prawns that is genetically related to the uukuniemi virus and other phleboviruses of the bunyaviridae. this paper describes an rt-nested pcr test that can reliably detect between 2 and 6 copies of a synthetic mov rna. total rna isolated from the lymphoid organ, gills and haemocytes of p. monodon with moderate infections gave comparable amplicon yields in the rt-pcr step of the test. however, in prawns with extremely low-level i ... | 2005 | 16231634 |
| development of gene transfer technology for black tiger shrimp, penaeus monodon. | an effective foreign gene transfer method for shrimp would have several potential uses in the shrimp culture industry, such as in preventing infectious diseases. we evaluated two gene transfer methods and used black tiger shrimp, penaeus monodon, as a model target species. for a promoter, we used the 1,592-bp promoter region of the ef-1alpha gene, a house-keeping gene, of kuruma shrimp marsupenaeus japonicus. the promoter region was linked to either the gene for green fluorescence protein (gfp) ... | 2005 | 16254920 |
| concentration-dependent suppressive effect of shrimp head protein hydrolysate on dehydration-induced denaturation of lizardfish myofibrils. | to utilize fishery waste products as functional food material, the shrimp head protein hydrolysate (shph) was produced from three species of shrimp wastes, northern pink shrimp, endeavour shrimp and black tiger shrimp, by enzymatic hydrolysis. the shph was used as a natural food preservative by adding to lizardfish myofibrils at concentrations ranging from 2.5% to 10%. their effects on the state of water and the denaturation of myofibrils during dehydration were evaluated. the amount of monolaye ... | 2006 | 16324842 |
| identification and characterization of a penaeus monodon lymphoid cell-expressed receptor for the yellow head virus. | the yellow head virus is a positive-sense, single-stranded rna virus that causes significant mortality in farmed penaeid shrimp. this study sought to isolate and characterize the receptor protein used by the virus to gain entry into penaeus monodon oka (lymphoid) organ cells, a primary target of yellow head virus infections. virus overlay protein binding assay on oka organ membrane preparations identified a 65-kda protein, and antibodies raised against this protein inhibited virus entry in prima ... | 2006 | 16352551 |
| complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (hpv) of penaeus monodon. | we have determined the genome of hepatopancreatic parvovirus (hpv), a minus, single-stranded dna virus isolated from infected penaeus monodon in thailand. its genome consisted of 6321 nucleotides, representing three large open reading frames (orfs) and two non-coding termini. the left (orf1), mid (orf2), and right (orf3) orfs on the complementary (plus) strand may code for 428, 579, and 818 amino acids, equivalent to 50, 68, and 92 kda, respectively. the 5' and 3' ends of viral genome contained ... | 2006 | 16356523 |
| purification, characterization and cdna cloning of a novel lipopolysaccharide-binding lectin from the shrimp penaeus monodon. | in invertebrates, c-type lectin plays an important role in innate immunity by mediating the recognition of pathogens to host cells and clearing microinvaders. a few c-type lectins have been identified from shrimps, but none of their gene or protein sequences is known to date. in this paper, a c-type lectin (named pmlec) specific for bacterial lipopolysaccharide was purified from the serum of the shrimp penaeus monodon. the binding of pmlec to lipopolysaccharide was mainly mediated through the o- ... | 2006 | 16364436 |
| molecular cloning and characterisation of prophenoloxidase cdna from haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, and its transcription in relation with the moult stage. | expression of prophenoloxidase (propo) cdna was determined from haemocytes of the giant freshwater prawn macrobrachium rosenbergii by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna using oligonucleotide primers based on the propo sequence of tiger shrimp penaeus monodon, freshwater crayfish pacifastacus leniusculus, green tiger shrimp penaeus semisulcatus, kuruma shrimp marsupenaeus japonicus, and white shrimp litopenaeus vannamei. the propo of m. rose ... | 2006 | 16376571 |
| molecular cloning and characterisation of peroxinectin, a cell adhesion molecule, from the giant freshwater prawn macrobrachium rosenbergii. | expression of peroxinectin cdna was determined from haemocytes of giant freshwater prawn macrobrachium rosenbergii using oligonucleotide primers and reverse transcription polymerase chain reaction (rt-pcr) based on the peroxinectin sequence of white shrimp litopenaeus vannamei, tiger shrimp penaeus monodon, and freshwater crayfish pacifastacus leniusculus. the peroxinectin of m. rosenbergii was constitutively expressed. analysis of the nucleotide sequence revealed that the cdna clone has an open ... | 2006 | 16377210 |
| a new rna virus found in black tiger shrimp penaeus monodon from thailand. | a new, apparently innocuous virus was found while investigating the cause of monodon slow growth syndrome (msgs) in cultured black tiger shrimp (penaeus monodon). it was identified via plasmid vector clones of e. coli containing randomly amplified cdna fragments produced from total nucleic acid extracts of hemolymph from msgs shrimp. of 421 clones, 30 that failed to give positive dot blot hybridization with a digoxigenin (dig)-labeled shrimp dna probe were sequenced and compared to genbank recor ... | 2006 | 16384619 |
| experimental infection of penaeus monodon with taura syndrome virus (tsv). | clinical signs and lesions of taura syndrome virus (tsv) infection in penaeus monodon have not been documented although the virus has been detected in this shrimp species by reverse transcription polymerase chain reaction (rt-pcr). this study provides the first evidence of tsv infection in p. monodon by histological and in situ hybridization (ish) analyses. we performed experimental bioassays with groups of p. monodon using inocula of p. monodon and litopenaeus vannamei (th04pmtsv and th04lvtsv, ... | 2005 | 16385801 |
| different responses to infectious hypodermal and hematopoietic necrosis virus (ihhnv) in penaeus monodon and p. vannamei. | infectious hypodermal and hematopoietic necrosis virus (ihhnv) is widespread in cultured penaeus monodon and p. vannamei in thailand. it causes runt-deformity syndrome that is characterized by physical abnormalities and stunted growth in p. vannamei, but causes no apparent disease in p. monodon. in both species, the virus may produce cowdry type a inclusions in tissues of ectodermal and mesodermal origin, but these are common in p. vannamei and rare in p. monodon. the virus can be more easily de ... | 2005 | 16408834 |
| yhv-protease dsrna inhibits yhv replication in penaeus monodon and prevents mortality. | yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. haemolymph injection of dsrna(pro) corresponding to the protease motif of yhv genome resulted in a complete inhibition of yhv replication. the effect of dsrna lasted for at least 5 days. injecting sequence-unrelated dsrna(gfp) or dsrna(tsv-pol) also resulted in an inhibition of yhv replication but at a comparatively much less extent. shrimp mortality was monitored for 10 days when more than ... | 2006 | 16426575 |
| shrimps survive white spot syndrome virus challenge following treatment with vibrio bacterin. | taking an innovative approach, a vaccination study using five bacterial strains viz. vibrio campbelli (b60), v. alginolyticus (b73), v. parahaemolyticus-like (b79), v. parahaemolyticus (r8) and v. harveyi (rg203) was conducted in penaeus monodon against white spot syndrome virus (wssv) infection, considered as one of the serious pathogens of shrimps. oral challenge with shrimps infected with wssv showed a relative percentage survival of 5 and 47% in the p. monodon juveniles vaccinated with v. pa ... | 2006 | 16430093 |
| differentially expressed genes in hemocytes of vibrio harveyi-challenged shrimp penaeus monodon. | differential display pcr technique (dd-pcr) was used for the analysis of altered gene expression in hemocytes of vibrio harveyi-infected penaeus monodon. forty-four combinations of arbitrary and oligo(dt) primers were used to screen for differentially expressed genes. a total of 79 differentially expressed bands could be identified from 33 primer combinations. these included 48 bands (61%) whose expression level increased and 31 bands (39%) decreased after v. harveyi challenge. subsequently, for ... | 2006 | 16466635 |
| identification of sex-specific expression markers in the giant tiger shrimp (penaeus monodon). | bulked segregant analysis (bsa) and aflp were used for isolation of genomic sex determination markers in penaeus monodon. a total of 256 primer combinations were tested against 6-10 bulked genomic dna of p. monodon. five and one candidate female- and male-specific aflp fragments were identified. female-specific fragments were cloned and further characterized. scar markers derived from fe10m9520, fe10m10725.1, fe10m10725.2 and fe14m16340 provided the positive amplification product in both male an ... | 2006 | 16466636 |
| infectious hypodermal and hematopoietic necrosis virus (ihhnv)-related sequences in the genome of the black tiger prawn penaeus monodon from africa and australia. | we found an infectious hypodermal and hematopoietic necrosis virus (ihhnv)-related sequence within the shrimp genome in populations of penaeus monodon from africa and australia. ihhnv is a single-stranded dna virus that has caused severe mortality and stunted growth in penaeid shrimp. recently, ihhnv-related sequences were found in samples of p. monodon from madagascar and tanzania. these sequences vary considerably (14 and 8%, respectively) from that of ihhnv found in association with viral epi ... | 2006 | 16473428 |