Publications

standardization of dot-elisa for the serological diagnosis of toxocariasis and comparison of the assay with elisa.	the dot-enzyme-linked immunosorbent assay (dot-elisa) was standardized using somatic (s) and excretory-secretory (es) antigens of toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. all samples were used before and after absorption with ascaris suum extract. when the results ...	1992	1307410
characterization of the major phosphofructokinase-dephosphorylating protein phosphatases from ascaris suum muscle.	in contrast to the mammalian enzyme, pfk from the nematode ascaris suum is activated following phosphorylation (daum et al. (1986) biochem. biophys. res. commun. 139, 215-221) catalyzed by a camp-dependent protein kinase (thalhofer et al. (1988) j. biol. chem. 263, 952-957). in the present report, we describe the characterization of the major pfk dephosphorylating phosphatases from ascaris muscle. two of these phosphatases exhibit apparent m(r) values of 174,000 and 126,000, respectively, and ar ...	1992	1321672
extracellular recordings from the motor nervous system of the nematode, ascaris suum.	1. the close association of muscle and neurons in ascaris suum makes it difficult to determine whether spikes recorded from nerve cords originate in muscle or neurons. we have developed criteria that distinguish muscle and neuronal activity. there are two categories of extracellular spikes. 2. the first category consists of spikes with a wide range of amplitudes, marked by large spikes. these spikes, which can be recorded over lateral muscle and over the dorsal and ventral nerve cords, are aboli ...	1992	1328624
the 5-lipoxygenase inhibitor zileuton blocks antigen-induced late airway responses, inflammation and airway hyperresponsiveness in allergic sheep.	leukotrienes are thought to be involved in allergen-induced airway responses. to test this hypothesis we used a newly described 5-lipoxygenase inhibitor, zileuton, and examined its effect on antigen-induced early and late bronchial responses, airway inflammation and airway hyperresponsiveness in allergic sheep. early and late responses were determined by measuring specific lung resistance (srl) before and serially for 8 h after antigen challenge. airway inflammation was assessed by bronchoalveol ...	1992	1330588
sk&f 104353, a selective leukotriene receptor antagonist, inhibits leukotriene d4- and antigen-induced bronchoconstriction in cynomolgus monkeys.	the ability of sk&f 104353 to prevent and reverse leukotriene (lt) d4- and antigen (ag)-induced bronchoconstriction was examined in anesthetized, spontaneously breathing cynomolgus monkeys. aerosol administration of ltd4 (10 micrograms/ml; 20 breaths) produced a sustained increase in pulmonary resistance and decrease in dynamic lung compliance. aerosolized sk&f 104353 (150 breaths, 0.3 or 4.4 mg/ml) administered 15 min prior to ltd4 challenge antagonized these changes in a dose-dependent manner. ...	1992	1332792
voltage-activated currents in somatic muscle of the nematode parasite ascaris suum.	1. voltage-activated currents in cell bodies of the somatic muscle cells of ascaris suum were studied using a two-microelectrode voltage-clamp technique. cells recorded from had resting membrane potentials around -35 mv and had input conductances in the range 1-10 micros. 2. in cells bathed in artificial perienteric fluid, depolarizing steps from a holding potential of -35 mv elicited outward currents at a threshold of -15 mv. these currents had inwardly directed inflections on the rising phase, ...	1992	1336792
pharmacological profiles of the gaba and acetylcholine receptors from the nematode, ascaris suum.	the pharmacological profiles of the acetylcholine and gaba receptors of ascaris muscle have been investigated. acetylcholine excites the muscle through activation of a nicotinic receptor which resembles the mammalian ganglionic receptor. dmpp is a potent agonist and alpha-bungarotoxin is a weak antagonist. gaba inhibits the muscle through activation of a chloride-linked receptor which in terms of agonist profile resembles the mammalian gaba-a receptor. however, bicuculline is inactive and picrot ...	1992	1338558
effect of purified components of ascaris suum extracts on ige antibody response in guinea pigs.	the effect of components p530 and p29, isolated from ascaris suum adult worm extract (asc), on the heterologous ige antibody response was studied in guinea pigs. groups of 7 guinea pigs were immunized ip with 50 micrograms of ovalbumin (oa) alone or mixed with 200 micrograms of each component precipitated in an alum gel. the primary and secondary ige antibody response was evaluated by passive cutaneous anaphylaxis reaction (pca). immunization of guinea pigs with p29 plus ovalbumin (oa) resulted ...	1992	1342594
identification of a novel 5-htn (nematoda) receptor from ascaris suum muscle.	1. the abilities of various serotonergic drugs to bind with the 5-ht receptor of ascaris suum muscle and to affect cyclic amp levels in muscle tissue were examined. 2. ligands which selectively interact with either the 5-ht1 or the 5-ht2 receptor in mammalian systems interact with the 5-ht receptor from a. suum muscle and increase cyclic amp levels. 3. no binding of 5-ht3 ligands to 5-ht receptors from a. suum muscle was observed. 4. the 5-ht receptor of a. suum muscle should be called the 5-htn ...	1992	1354123
chromatographic and immunological characterisation of neuropeptide y-like and pancreatic polypeptide-like peptides from the nematode ascaris suum.	1. immunoreactivity (ir) towards neuropeptide y (npy) and pancreatic polypeptide (pp) has previously been demonstrated in nematodes by immunocytochemistry (icc). 2. the pp- and npy-ir in the nematode parasite ascaris suum were analysed using hplc and radioimmunoassays (rias) specific for either peptide. 3. quantitative ria data on the distribution of these irs in the parasite disagreed with icc data suggesting that different tissue treatments used in the two techniques lead to different antigen ...	1992	1360356
the pyruvate dehydrogenase complex from the parasitic nematode ascaris suum: novel subunit composition and domain structure of the dihydrolipoyl transacetylase component.	the pyruvate dehydrogenase complex (pdc) from muscle of the adult parasitic nematode ascaris suum plays a unique role in its anaerobic mitochondrial metabolism. resolution of the intact complex in high salt dissociates the pyruvate dehydrogenase subunit but leaves the dihydrolipoyl dehydrogenase subunit (e3) and two other proteins with apparent m(r)s of 45 and 43 kda bound to the dihydrolipoyl transacetylase (e2) core. these proteins are not observable on coomassie brilliant blue-stained gels of ...	1992	1376597
a monoclonal antibody-based immunodiagnostic assay for onchocerciasis.	five murine monoclonal antibodies raised against onchocerca volvulus cuticular extracts and termed movs (1-4 and 6) were selected based on reactivity with o. volvulus cryosections, and non-reactivity with cryosections of human skin and/or nodular tissue. two others movs 5 and 7 reacted with both. using the peroxidase-anti- peroxidase (pap) histochemical method, the target epitopes of mov 1 were located in the cuticle's basal and cortical layers, those of mov 2 in the cortical layer; whilst mov 3 ...	1992	1381517
immunologic mast cell-mediated responses and histamine release are attenuated by heparin.	heparin has been shown to act as a competitive inhibitor of inositol 1,4,5-triphosphate (insp3) receptors in various cell types. because insp3 is one of the second messengers involved in stimulus-secretion coupling in mast cells, it is possible that heparin may inhibit mast cell-mediated reactions. therefore, in allergic sheep, we tested this hypothesis in two mast cell-mediated reactions induced by immunologic and nonimmunologic stimuli: immediate cutaneous reaction (icr) and acute bronchoconst ...	1992	1383185
patent infections of ascaris suum in pigs: effect of previous exposure to multiple, high doses of eggs and various treatment regimes.	fifty-four crossbred, 4-week-old pigs divided into nine equal groups were used to test whether multiple inoculations with high numbers of a. suum eggs with or without anthelmintic would result in patent infections. all pigs exposed to multiple prechallenge inoculations of 500, 1000, 2000, 5000, 10,000 and 20,000 and challenged orally 2 weeks later with 10,000 eggs harboured adult worms. when prechallenge infections were removed by pyrantel tartrate treatment the animals were more susceptible to ...	1992	1399243
inhibitory reflex during bronchoconstrictions induced by histamine and ascaris suum antigen.	we investigated the involvements of sympathetic and nonadrenergic nervous systems in the inhibitory reflex following bronchoconstriction in dogs. inhalations of a 0.00125% solution of histamine and ascaris suum antigen (3 mg protein) to the bronchial side induced reflex tracheal constriction following bronchoconstriction. an intra-arterial infusion of 5 micrograms/min of atropine to the tracheal site changed the reflex tracheal constrictions by histamine and antigen inhalations into tracheal dil ...	1992	1405031
immunoreactivity to two specific regions of chromogranin a in the nervous system of ascaris suum: an immunocytochemical study.	antisera to a highly conserved region of chromogranin a (sequence keltae) and to a hexapeptide (sequence kgqele) adjacent to the putative c-terminus of pancreastatin, a peptide whose sequence is found within the chromogranin a molecule, have been used to examine the localisation of immunoreactivity (ir) to these peptides in ascaris suum. ir to both peptides was found in the nerve rings and nerve cords. in addition, kgqele-ir was also observed in the pharyngeal neurones and in a network of fibres ...	1992	1409533
distribution of a fluorescent ivermectin probe, bodipy ivermectin, in tissues of the nematode parasite ascaris suum.	a fluorescent derivative of the anthelmintic ivermectin (4''-5,7-dimethyl bodipy proprionylivermectin, referred to hereafter as bodipy ivermectin) was synthesized for an investigation of the distribution of avermectins. injected into adult ascaris suum at doses greater than 0.1 micron per worm, it produced a dose-dependent immobilization. fluorescent microscopy of frozen sections revealed the distribution of the probe in the whole nematode. staining of collagenase-isolated muscle cells was studi ...	1992	1409535
development of an animal model of late asthmatic response in guinea pigs and effects of anti-asthmatic drugs.	we developed an animal model of late asthmatic response (lar) in guinea pigs and examined the effects of anti-asthmatic drugs and peptide leukotriene antagonist, mci-826, on this model. bronchial challenge of dnp-as (dinitrophenylated-ascaris suum extract)-sensitized guinea pigs induced a biphasic increase in pulmonary resistance (rl) with the maximal increase being observed immediately (iar, immediate asthmatic response) and 3 to 5 hr after antigen inhalation (lar). twelve of 22 guinea pigs sho ...	1992	1410517
detection of circulating antigens in human trichinellosis.	a 'sandwich' enzyme-linked immunosorbent assay was established to detect circulating antigens of trichinella spiralis in human sera, its sensitivity and specificity was evaluated using 4 antigens (trichinella spiralis, trichuris trichiura, dirofilaria immitis and ascaris suum), and it was found to be sensitive and specific for t. spiralis antigen. samples of 347 individuals with suspected trichinellosis, who had eaten incompletely cooked bear meat containing larvae of t. spiralis, were examined. ...	1992	1412659
the primary structure of te-6: a novel neuropeptide from the nematode ascaris suum.	extensive immunoreactivity (ir) towards a hexapeptide (sequence kgqele), which flanks the c-terminus of the pancreastatin sequence in rat chromogranin a (cga), is found throughout the nervous system of the nematode parasite ascaris suum. the peptide ir was purified from the gonoduct of the parasite and found to have the sequence tkqele. this peptide, designated te-6, has some c-terminal homology with several regions of the cga molecule. however, te-6 was the only peptide isolated suggesting that ...	1992	1417808
interaction between ascaris suum and pasteurella multocida in the lungs of mice.	in an experiment including 8 groups of 15 mice, the effect of migrating ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to pasteurella multocida was investigated. following aerosol exposure to p. multocida, mice with migrating a. suum in their lungs developed more severe pneumonia and septicaemia than did parasite-free mice. the parasite-induced effect on bacterial pathogenicity was more marked for a non-toxin-producing p. multocida as compared with a ...	1992	1438140
pulmonary vascular response to anaphylaxis in isolated canine lungs.	we determined changes in vascular resistance and microvascular permeability during anaphylactic reaction in isolated canine lungs perfused at constant pressure with autologous blood. in lungs with anaphylaxis induced by an intra-arterial injection of ascaris suum antigen (10 mg), pulmonary vascular resistance and capillary pressure assessed as double occlusion pressure increased transiently by 10 times and 6.3 mmhg, respectively. pre- to postcapillary vascular resistance ratio decreased from 0.8 ...	1992	1443218
mechanism of activation of the nad-malic enzyme from ascaris suum by fumarate.	the mechanism of activation of the nad-malic enzyme from ascaris suum by fumarate has been probed using initial velocity studies, deuterium isotope effects, and isotope partitioning of the e:mg:malate complex. fumarate exerts its activating effect by decreasing the off-rate for malate from the e:mg:malate and e:nad:mg:malate complexes. fumarate is a positive heterotropic effector of the nad-malic enzyme at low concentrations (k act approximately 0.05 mm) and an inhibitor competitive against mala ...	1992	1444459
relationship of airway responsiveness to agents causing bronchoconstriction and cough in sensitized guinea-pigs.	the relationship between airway responsiveness to bronchoconstrictor- and cough-inducing stimuli has been examined in ascaris suum-sensitized conscious guinea-pigs. guinea-pigs were sensitized to ascaris suum [4000 pnu and 100 mg al(oh)3 i.p. on days 1 and 7] and then challenged with aerosolized antigen on days 21, 28 and 35. at day 35, antigen-exposure produced an early bronchoconstrictor response (ebr) and in about 50% of the animals also a late bronchoconstrictor response (lbr) commencing 4-8 ...	1992	1446141
ascaris hemoglobin gene: plant-like structure reflects the ancestral globin gene.	animal globin genes have two introns at strictly conserved positions, while plant globin genes have both of these as well as an additional, central intron. it has been proposed that a common ancestor gene had three introns, one of which was subsequently lost from animal but not plant globin genes. we have elucidated the cdna sequence and gene structure of a hemoglobin from the parasitic nematode ascaris suum and found a plant-like central intron, providing strong evidence for a three-intron ance ...	1992	1465385
ascaris suum--vaccination of mice with liposome encapsulated antigen.	vaccination with liposome encapsulated adult crude antigen with and without coencapsulated immunomodulator (levamisole) in a mice/larval ascaris suum model provided protection against a challenge infection (2000 eggs) in mice immunised by immobilised antigen. the best results (88.9% protection) were obtained with a combination of two doses of liposome entrapped antigen with leamisole. vaccination with liposome vaccine without modulator was slightly less effective (78.7% protection). a single dos ...	1992	1496793
association of a heat-stable inhibitor protein with cyclic-3',5'-amp-dependent protein kinase from the nematode ascaris suum: purification and characterization of the inhibitor.	an inhibitor protein of the catalytic subunit of the cyclic 3',5'-amp-dependent protein kinase from the nematode ascaris suum was isolated and characterized. the molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on superose 12. the trypsin-labile inhibitor was resistant to short incubations (less than or equal to 5 min) at temperatures up to 95 degrees c and at ph 3. it affected the protein kina ...	1992	1497350
mechanisms of microenvironmental ph regulation in the cuticle of ascaris suum.	the excretion kinetics of various organic acids by ascaris suum were quantified to determine if the excretion of these metabolic end-products could generate and maintain a microclimate ph within the aqueous compartment of the cuticle. ligated and nonligated a. suum were incubated in media buffered with 0.25 or 2.5 mm hepes (initial ph 7.5) or 0.5 or 5 mm glycine (initial ph 3.25). the concentration of organic acids and the ph of the media were followed for 24 h. several volatile fatty acids, inc ...	1992	1501633
structure and macromolecular assembly of two isoforms of the major sperm protein (msp) from the amoeboid sperm of the nematode, ascaris suum.	ascaris sperm are amoeboid cells that crawl by extending pseudopods. although amoeboid motility is generally mediated through an actin-based cytoskeleton, ascaris sperm lack this system. instead, their major sperm protein (msp) forms an extensive filament system that appears to fulfil this function. because their motility appears to be essentially the same as that of their actin-rich counterparts, ascaris sperm offer a simple alternative system for investigation of the molecular mechanism of amo ...	1992	1527183
resistance to ascaris suum in parasite naïve and naturally exposed growers, finishers and sows.	commercially reared growers, finishers, and sows of danish landrace x yorkshire crossbred were inoculated orally with ascaris suum at 50 eggs kg-1 body weight. white spots on the serosal surface of livers and total larval recoveries from lungs were recorded 7 days later. the response in pigs originating from a specific pathogen free and parasite free herd (parasite naïve) was observed in the three different age groups and compared with age-matched pigs from a herd maintained in a facility contam ...	1992	1532874
response to repeated inoculations with ascaris suum eggs in pigs during the fattening period. i. studies on worm population kinetics.	this experimental study on pigs was designed to simulate natural, long-term exposure to ascaris suum under modern management conditions. parasite kinetics were followed in pigs receiving a. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg (baconers). in pigs inoculated twice weekly with 500 eggs, there was an initial marked rise in the numbers of hepatic milk spots, but as early as around week 6 after the start of in ...	1992	1534170
effects of inhaled heparin on immunologic and nonimmunologic bronchoconstrictor responses in sheep.	the glycosaminoglycan heparin has been shown to block inositol triphosphate (ip3) receptors in various tissues. because ip3 is one of the pathways involved in stimulus-secretion coupling in mast cells, it is possible that heparin may inhibit airway anaphylaxis. thus, the purpose of the present investigation was to study the effect of inhaled heparin on immunologically and nonimmunologically induced bronchoconstriction in allergic sheep. specific lung resistance (srl) was measured in 15 sheep all ...	1992	1546836
the mitochondrial genomes of two nematodes, caenorhabditis elegans and ascaris suum.	the nucleotide sequences of the mitochondrial dna (mtdna) molecules of two nematodes, caenorhabditis elegans [13,794 nucleotide pairs (ntp)], and ascaris suum (14,284 ntp) are presented and compared. each molecule contains the genes for two ribosomal rnas (s-rrna and l-rrna), 22 transfer rnas (trnas) and 12 proteins, all of which are transcribed in the same direction. the protein genes are the same as 12 of the 13 protein genes found in other metazoan mtdnas: cyt b, cytochrome b; coi-iii, cytoch ...	1992	1551572
motor behavior and motor nervous system function in the nematode ascaris suum.	ascaris suum has a nervous system that is very simple both numerically and morphologically. it comprises only 298 neurons almost all of which are extremely simple in shape. extensive anatomical descriptions of the morphology of neurons and of their synaptic connections, together with the study, by using intracellular recording techniques, of their physiological properties, have led to a prediction of how the motor nervous system controls behavior. subsequent discovery of endogenous neuropeptides ...	1992	1556637
comparison between the mhc-restricted antibody repertoire to ascaris antigens in adjuvant-assisted immunization or infection.	genetic restrictions to the immune repertoire will be an important consideration in the development of anti-nematode vaccines. it has already been established that the major histocompatibility complex (mhc) limits responsiveness to nematode antigens in infection, but little is known of whether this also applies under other routes of sensitization, such as with adjuvants. the specificity of the antibody response was, therefore, compared in infection and adjuvant-assisted immunization using secret ...	1992	1557231
effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs.	in experimental ascariosis (ascaris suum) of pigs a preparation of glucan combined with pig immunoglobulin and zinc was found to significantly elevate the levels of both t and b lymphocytes in the blood, and to significantly stimulate the phagocytic ability of monocytes and neutrophilic granulocytes in experimental pigs. the protective effect of the preparation, assessed by the reduction in the number of migrating ascarid larvae in the lungs of pigs was 65%.	1992	1561757
characterization of cdna clones for the alpha subunit of pyruvate dehydrogenase from ascaris suum.	the pyruvate dehydrogenase complex of the adult parasitic nematode ascaris suum functions in the reducing environment present in their anaerobic mitochondria. these organelles use fumarate and enoyl coas as terminal electron acceptors instead of oxygen. a lambda gt11 cdna library was constructed from rna isolated from adult ascarid muscle. partial clones for the pyruvate dehydrogenase alpha subunit were isolated by screening the lambda gt11 library with a specific antiserum. full-length clones ( ...	1992	1565136
purification and characterization of polyamine oxidase from ascaris suum.	the interconversion of polyamines in the parasite nematode ascaris suum by a novel type of polyamine oxidase was demonstrated. the nematode enzyme was clearly distinguishable from monoamine and diamine oxidases as well as from the mammalian polyamine oxidase, as shown by the use of the specific inhibitors pargyline, aminoguanidine and mdl 72527 respectively. all three inhibitors had no effect on the parasite polyamine oxidase, and the enzyme did not accept diamines such as putrescine, cadaverine ...	1992	1567380
the onset and recovery from airway hyperresponsiveness: relationship with inflammatory cell infiltrates and release of cytotoxic granule proteins.	previous studies from our laboratory have demonstrated a temporal relationship between eosinophil influx into the airways and the onset of airway hyperresponsiveness to inhaled methacholine. the purpose of the present study was to extend this observation by evaluating changes in airway cellular composition and measuring the levels of granulocyte-derived mediators recovered in bal fluid during the onset and recovery from antigen-induced airway hyperresponsiveness. airway cellular composition, air ...	1992	1571822
purification of glutathione reductase from muscle of the adult parasitic nematode ascaris suum.		1992	1574090
isolation of ascaris suum components which suppress ige antibody responses.	an extract from adult ascaris suum was fractionated on a sephadex g-200 column and individual elution fractions assessed for their ability to induce or suppress an ige antibody response in mice immunized simultaneously with a heterologous antigen (ovalbumin). the results showed that, whilst fractions eluted in the first peak suppressed the anti-ovalbumin ige antibody production, those eluted in the third peak induced a significant anti-ascaris ige response. the mixture of the two kinds of fracti ...	1992	1582696
vascular permeability and airway narrowing during late asthmatic response in dogs treated with metopirone.	recently, we have developed an animal model of late asthmatic response (lar) by treating naturally sensitized dogs to ascaris suum antigen with the cortisol-synthesizing inhibitor, metopirone. by using this animal model, we examined the contribution of edema in the airway wall to the development of lar. to study whether airway microvascular leakage is increased in association with lar, we performed antigen challenge in dogs treated with metopirone. we measured the amount of extravasated evans bl ...	1992	1583251
polar zipper sequence in the high-affinity hemoglobin of ascaris suum: amino acid sequence and structural interpretation.	the extracellular hemoglobin of ascaris has an extremely high oxygen affinity (p50 = 0.004 mmhg). it consists of eight identical subunits of molecular weight 40,600. their sequence, determined by protein chemistry, shows two tandemly linked globin-like sequences and an 18-residue c-terminal extension. two n-linked glycosylation sites contain equal ratios of mannose/glucosamine/fucose of 3:2:1. electron micrographs suggest that the eight subunits form a polyhedron of point symmetry d4, or 42. the ...	1992	1584800
efficacy of an in-feed preparation of ivermectin against endoparasites and scabies mites in swine.	in 2 trials, the efficacy of an in-feed preparation of ivermectin was evaluated in 40 pigs naturally infected with endoparasites and sarcoptes scabiei var suis. treated pigs (n = 10 in each trial) were fed a ration containing 2 ppm ivermectin for 7 days, followed by consumption of a nonmedicated ration for the remainder of the trial. control pigs (n = 10 in each trial) were fed a complete, nonmedicated ration for the duration of the trial. pigs in trial a were monitored for 14 days after treatme ...	1992	1586019
effects of cholinergic agents on the metabolism of choline in muscle from ascaris suum.	the incorporation of [methyl-14c]choline into the choline-containing compounds of ascaris suum muscle and the effects of acetylcholine and its agonists, carbachol and levamisole, on this incorporation were studied. previous experiments reported a stimulation of phosphatidylcholine (lecithin) metabolism upon the administration of acetylcholine. acetylcholine administered in vitro to a. suum muscle and body wall preparations resulted in a stimulation of phospholipase c activity that, in turn, prod ...	1992	1597777
[ascaris suum infection as a cause of clinical problems in cattle?].	a sudden decrease in milk yield, increased respiratory rate and occasional coughing were observed in dairy cows on two farms in spring 1991. pigs were also kept on these farms, and pastures grazed by the cattle had been fertilised with pig slurry. laboratory investigations of some of the cattle showed eosinophilia and high elisa titres of antibodies against ascaris suum. on one farm the clinical symptoms disappeared after the animals had been treated with oxfendazole and on the other farm the sy ...	1992	1604447
immunoepidemiology of ascaris lumbricoides: relationships between antibody specificities, exposure and infection in a human community.	the serum antibody responses of 124 people naturally exposed to ascaris lumbricoides infection were analysed by immunoprecipitation of radio-isotope labelled 3rd- and 4th-stage larval ascaris suum excretory and secretory antigens (l3/4 es). profiles of antigens recognized were visualized by polyacrylamide gel electrophoresis (sds-page), and the band intensities of the 12 major precipitated antigens were individually scored. most subjects were seropositive, but considerable variation was observed ...	1992	1614731
mechanistic studies in the transcuticular delivery of antiparasitic drugs. ii: ex vivo/in vitro correlation of solute transport by ascaris suum.	using live, intact ascaris suum and a closed perfusion system, the absorption kinetics and tissue distribution of selected radiolabeled permeants were measured to determine the importance of the transcuticular pathway for drug absorption. the data support the conclusions established by previous in vitro transport studies which utilized excised cuticle-hypocuticle tissue preparations. the external surface of a. suum can be breached by drugs and the rate-determining barrier is the lipoidal hypocut ...	1992	1625697
dna synthesis in the early embryo of the nematode ascaris suum.	we have used microspectrofluorimetry to measure the rate of dna synthesis in the first two embryonic cell cycles of the parasitic nematode ascaris suum. the s-phase of the early ascaris cell cycles occupies at most 1 hr; g2 phase is prominent and occupies approximately 11 hr; no g1 phase could be detected. these results contrast with our previous measurements made with embryos of the free-living nematode caenorhabditis elegans, in which the earliest cell cycles consist of simple alternations bet ...	1992	1628758
ascaris suum: are trypsin inhibitors involved in species specificity of ascarid nematodes?	inhibitors of porcine trypsin were prepared from aqueous extracts of the parasitic nematodes ascaris suum (hogs) and ascaris lumbricoides (human). in this study three experiments were performed. (1) polyclonal antibodies were prepared against one isoform of trypsin inhibitor from each parasitic nematode. each antibody reacted with all isoforms from itself as well as all isoforms from the other parasite. (2) association equilibrium constants were measured by titrating host trypsins (porcine or hu ...	1992	1639157
cross-reactions of sera from toxocara canis-infected mice with toxascaris leonina and ascaris suum antigens.	the elisa method using larval excretory-secretory (e/s) products and homogenized toxocara canis, toxascaris leonina and ascaris suum adult worm extract were used to determine possible cross-reactions in balb/c and c57bl/10 mice, inoculated with embryonated eggs or adult worm extract of t. canis in single and multiple doses. when we used sera of mice infected with embryonated eggs of t. canis against different heterologous antigens, we observed no cross-reactions in balb/c mice against a. suum e/ ...	1992	1639565
crystallization of the nad-dependent malic enzyme from the parasitic nematode ascaris suum.	the malic enzyme from muscle mitochondria of the parasitic nematode ascaris suum is a tetramer of 65 kda monomers that catalyzes the oxidative decarboxylation of malate to pyruvate and co2 with nad cofactor as oxidant. this malic enzyme is critical to the nematode for muscle function under anaerobic conditions. unlike mammalian versions of the enzyme such as that found in rat liver, which require nadp as cofactor, the nematode version is an nad-dependent enzyme. we report the crystallization of ...	1992	1640469
on the distribution of a fluorescent ivermectin probe (4" 5,7 dimethyl-bodipy proprionylivermectin) in ascaris membranes.	the distribution of the fluorescent ivermectin derivative (4" 5,7 dimethyl bodipy proprionylivermectin, referred to here as bodipy ivermectin) was studied in muscle vesicle membranes from the parasite ascaris suum. incubation in bodipy ivermectin, at concentrations greater than 1 ng/ml, produced a dose-dependent fluorescence in the vesicles. the level of fluorescence was not depressed (but increased) by prior incubation with 1 microm unlabelled ivermectin. this observation suggested that most of ...	1992	1641253
the physiology and pharmacology of neuromuscular transmission in the nematode parasite, ascaris suum.	the organization of ascaris motoneurones and nervous system is summarized. there is an anterior nerve ring and associated ganglia, main dorsal and ventral nerve cords which run longitudinally, and a small set of posterior ganglia. cell bodies of motoneurones are found in the ventral nerve cord and occur in 5 repeating 'segments'; each contains 11 motoneurones. seven morphological types of excitatory or inhibitory motoneurone are recognized. each ascaris somatic muscle cell is composed of the con ...	1991	1647516
antigen-induced mediator release in primates.	we examined the release of bronchoactive mediators into the airways of allergic primates during the acute response to specific antigen inhalation. twelve adult male cynomolgus monkeys (macaca fascicularis) with a naturally occurring respiratory sensitivity to inhaled ascaris suum extract were anesthetized and intubated for each study. respiratory system resistance (rrs) and dynamic lung compliance (cldyn) were measured before and after antigen inhalation, and the release of mediators into the ai ...	1991	1648317
characterization of sulfidopeptide leukotriene responses in sheep tracheal smooth muscle in vitro.	we have investigated the effects of leukotrienes (lts) on isolated tracheal smooth muscle from sheep sensitive to ascaris suum antigen. ltc4 and ltd4 produced dose-dependent contractions of sheep trachea, but lte4 was virtually inactive. ym-17690, a non-analogous lt agonist, produced no contractile response up to 100 microm. indomethacin (5 microm) had no effect on ltc4- and ltd4-induced contractions. l-serine borate (45 mm), an inhibitor of gamma-glutamyl transpeptidase, shifted the dose-respon ...	1991	1655181
antagonist properties of arylaminopyridazine gaba derivatives at the ascaris muscle gaba receptor.	1. in a previous study, it was shown that the potency order for two arylamino-pyridazine derivatives, sr95531 and sr95103, was different in ascaris suum when compared to vertebrate preparations. sr95531, the most potent analogue at the vertebrate gabaa receptor, was found to be very weak at antagonizing gaba responses in ascaris, but sr95103, approximately 20 times less potent than sr95531 in vertebrate preparations, was more potent than sr95531 in ascaris. these results suggested the existence ...	1991	1658181
novel regulation of pyruvate dehydrogenase phosphatase activity from mitochondria of the parasitic nematode, ascaris suum.		1991	1664050
hallucinogenic and neuroleptic drug interactions with potential neurotransmitter receptors in parasitic nematodes.	receptors potentially involved in neurotransmitting have been characterised in the muscle tissue and in whole worms of the nematodes ascaris suum and onchocerca volvulus, respectively. binding studies revealed a high affinity for lsd with apparent kd values of 94 nm for a. suum and 120 nm for o. volvulus, whereas those of the neuroleptics haloperidol, spiperone and mianserin were found to be in the micromolar range. a variety of neurotransmitter antagonists, known to bind with high affinities ei ...	1991	1674821
effects of plumbagin on development of the parasitic nematodes haemonchus contortus and ascaris suum.	1. plumbagin (5-hydroxy,2-methyl-1,4-napthoquinone) inhibited the motility and survival of haemonchus contortus first-stage larvae (l1) with an ed50 of 1 microgram/ml, but was less effective in preventing the development of h. contortus to infective third-stage larvae in a faecal slurry assay. 2. of the structural analogs tested, plumbagin was the most potent in preventing development of l1 followed in decreasing order of potency by 1,4-naphthoquinone, 5-hydroxy-1,4-napthoquinone (juglone) and 1 ...	1991	1687553
identification of the major ascaris allergen and its purification to homogeneity by high-performance liquid chromatography.	the major allergen from the body fluid of adult ascaris suum (abf) has been identified and purified to homogeneity using gel permeation high-performance liquid chromatography (hplc). the purity of the ascaris body fluid allergen (aba-1) was confirmed by hplc and sds-page. aba-1 appears to be a 25-kda dimer in its native form, which runs as a 10-kda monomer on sds-page under reducing conditions. the allergenicity of the hplc-purified protein was confirmed using isolated in vivo-sensitised mast ce ...	1990	1690856
surface properties of membrane vesicles prepared from muscle cells of ascaris suum.	to facilitate biochemical, pharmacological, and biophysical studies on the membrane of the body muscle of ascaris suum, a method for preparing intact vesicles was developed. vesicles were prepared by incubating a muscle flap preparation with 1 mg/ml collagenase in a saline solution and then washing in saline without enzyme. the vesicles then formed gradually over the next hour as outgrowths of the original surface membrane from the bag region of the muscle. the vesicles were harvested readily by ...	1990	1693673
characterization of monoclonal antibodies to young-adult worms of angiostrongylus cantonensis.	three igg1 and one igg2a monoclonal antibodies were obtained by fusion of ns-1 cells with spleen cells of balb/c mice immunized with young-adult worm antigen of a. cantonensis. these 4 monoclonal antibodies were specific to a. cantonensis without cross-reactivity to any antigens of toxocara canis, ascaris suum, clonorchis sinensis, dirofilaria immitis, paragonimus westermani, gnathostoma spinigerum, strongyloides stercoralis and anisakis spp. detected by elisa. the affinity constants of monoclon ...	1990	1701759
a patch-clamp study of acetylcholine-activated ion channels in ascaris suum muscle.	acetylcholine-activated single-channel currents were recorded from cell-attached and inside-out patches of isolated muscle vesicles from ascaris suum. acetylcholine (1-10 mumols l-1) activated cation-selective channels of two amplitudes: 40-50 ps and 25-35 ps. both channels had linear i/v relationships and mean open durations independent of voltage. the larger conductance was analysed in detail to determine its open-, closed- and burst-time kinetics; the open and burst durations were composed of ...	1990	1703557
airway effects of inhaled bradykinin, substance p, and neurokinin a in sheep.	the effects of inhaled bradykinin (bk), substance p (sp), and neurokinin a (nka) on pulmonary resistance and airway responsiveness to carbachol were studied in conscious allergic sheep. inhaled bk (20 breaths, 0.1 to 5.0 mg.ml-1) caused dose-dependent increases in pulmonary resistance. neither inhaled sp nor nka (20 breaths, 0.1 to 1.0 mg.ml-1) produced significant bronchoconstriction in allergic sheep. however, the response to sp could be enhanced (p less than 0.05) by pretreatment with the neu ...	1991	1704388
the complete primary structure of a nematode alpha 2(iv) collagen and the partial structural organization of its gene.	we have isolated and characterized cdna and genomic dna clones which encode an alpha 2(iv) collagen chain from the parasitic nematode ascaris suum. in addition we have determined, by nucleic acid sequence analysis, the structural organization of approximately two-thirds of the gene. this analysis has shown that the gene contains at least 15 introns, and those that have been characterized range in size from 141 to 854 base pairs. the derived protein sequence contains 1763 amino acids and includes ...	1991	1714907
leukotrienes, thromboxane a2, and prostaglandins during systemic anaphylaxis in sheep.	we investigated the roles of eicosanoid mediators in acute systemic anaphylaxis in anesthetized sheep. sheep were sensitized with dinitrophenylated ascaris suum extract and were challenged with an intravenous injection of dinitrophenylated bovine serum albumin. during anaphylaxis, cyclooxygenase inhibitors eliminated the elevation of arterial plasma levels of thromboxane b2 and 6-ketoprostaglandin f 1 alpha but markedly elevated the levels of leukotriene e4 in lung lymph without significantly el ...	1991	1716064
studies on the ionic selectivity of the gaba-operated chloride channel on the somatic muscle bag cells of the parasitic nematode ascaris suum.	the reversal potential for the gaba-elicited hyperpolarization (egaba) of the muscle bag cells of the parasite nematode ascaris suum is near to the equilibrium potential for chloride ions (ecl) indicating that this is a chloride-mediated event. the value of egaba in the presence of different anions indicates the selectivity sequence of the gaba-operated chloride channel as scn- greater than br-, i- greater than clo3-, no3-, cl- greater than c2h5coo- greater than ch3coo-, hcoo-, ohch2ch2so3h-, ch ...	1991	1716924
larval exoantigens from ascarid nematodes are potent inducers of histamine release from human blood basophils.	histamine release (hr) from washed human blood cells after challenge with the excretory-secretory antigens (es) of the parasitic nematodes toxocara canis and ascaris suum was studied, employing a recently developed microfibre-based assay combined with hyperosmolar release media for maximal sensitivity. blood samples were obtained from 30 patients suspected of parasite infection and 11 healthy volunteers serving as controls. specific antibodies of ige, igg1 and igg4 subclasses were determined by ...	1991	1723345
structure of mitochondrial trna.	some mitochondrial (mt) trnas are presumed to have unusual secondary structures on the basis of dna sequence analyses. however there are few trnas whose structures have been investigated at rna level. thus we isolated some mt trnas to investigated their structures and also synthesized mt trna by run-off transcription. in these experiments, following results have been obtained. 1) bovine mt trna(ser)(agy) needs the interaction between the t-loop and d-arm for its aminoacylation activity. 2) the s ...	1991	1726807
biochemical characterisation of the enzyme responsible for "activated l-serine sulphydrase" activity in nematodes.	the biochemical properties of the enzyme responsible for nematode "activated l-serine sulphydrase" activity (l-cysteine + r-sh----cysteine thioether + h2s) have been investigated using primarily the gastro-intestinal nematodes nippostrongylus brasiliensis and haemonchus contortus. the activated l-serine sulphydrase enzyme was found to be cytosolic in origin and exhibited maximal activity at ph 9.0. enzyme activity was widely distributed amongst the major tissues of adult female ascaris suum but ...	1992	1740180
in vitro damaging effect of leukocytes, serum, and peritoneal fluid on ascaris suum infective larvae.	peritoneal leukocytes, serum, and peritoneal fluid from ascaris suum-infected or noninfected mice were studied using 51cr release and larval mobility to determine if they had in vitro cytotoxicity for a. suum infective larvae. mouse serum or peritoneal fluid from infected and non-infected mice was added to leukocyte-larvae cultures to determine their effect on leukocyte damage to larvae. leukocytes from a. suum infected mice were cytotoxic for a. suum larvae in the presence of serum from infecte ...	1991	1741924
morphological and morphometric studies of the airways of sheep with acute airway hypersensitivity to inhaled ascaris suum.	the airways of 12 sheep with naturally-occurring allergic airway hypersensitivity, six of which had changes in both airway resistance and dynamic lung compliance (group a) and six of which had changes in only dynamic lung compliance (group b), were compared quantitatively with six non-reacting sheep (group c) in order to examine the relation between airway hypersensitivity and various morphological features thought to be related to airway hypersensitivity. compared to the non-reacting sheep (gro ...	1991	1742207
lysophospholipase activity of ascaris suum-induced mouse peritoneal neutrophils and eosinophils.	lysophospholipase activity of mouse peritoneal neutrophils and eosinophils was studied to determine if neutrophils and eosinophils have lysophospholipase activity when treated with ascaris suum whole worm extract, if zymosan activated complement can induce increased lysophospholipase activity, or if the immune status of the host has an effect on lysophospholipase activity. neutrophils from noninfected or infected (immunized) mice were found to have increased lysophospholipase activity when treat ...	1991	1753875
phospholipids and protein kinase c in acetylcholine-dependent signal transduction in ascaris suum.	quantitatively, the major phospholipid in the muscle of the nematode ascaris suum was found to be phosphatidylcholine (lecithin). stimulation of ascaris muscle with acetylcholine or the agonists carbachol and levamisole increased the level of phosphorylcholine, 1,2-diacylglycerides and phosphatidic acid. increased levels of these compounds, together with the demonstration of phospholipase c activity, suggest that phospholipid hydrolysis may be associated with the ach response of the muscle via s ...	1991	1762627
structure of the polypeptide chain of extracellular hemoglobin from the nematode ascaris suum.	1. ascaris suum extracellular hemoglobin is composed of eight identical single polypeptide chain subunits carrying two heme binding sites each. 2. limited trypsinolysis followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis gave a major band corresponding to half the molecular mass of an intact subunit. 3. peptide mapping of tryptic hydrolysates yielded 27 to 30 fluorescamine positive spots, about half the number of lysyl and arginyl residues in a polypeptide chain. 4. the findin ...	1991	1764923
centripetal flow and directed reassembly of the major sperm protein (msp) cytoskeleton in the amoeboid sperm of the nematode, ascaris suum.	the cytoskeleton of the amoeboid spermatozoa of ascaris suum consists of major sperm protein (msp) filaments arranged into long, branched fiber complexes that span the length of the pseudopod and treadmill rearward continuously due to assembly and disassembly at opposite ends of the complexes (sepsenwol et al., journal of cell biology 108:55-66, (1989)). examination by video-enhanced microscopy showed that this cytoskeletal flow is tightly coupled to sperm locomotion. the fiber complexes treadmi ...	1991	1773449
[effects of immunoactivity on ascaris suum infection in mice].	the immune response to sheep red blood cell (srbc) was monitored in the mice infected with ascaris suum or trichinella spiralis. the effects of the infection with t. spiralis or the injection with cyclophosphamide(cy) as an immunosuppression agent prior to challenge infection with the embryonated eggs of a. suum were monitored in mice by means of the level of infection with a. suum and cellular and humoral immune response to srbc. following the oral administration of 1,000 eggs of a. suum to mic ...	1991	1786254
identification of tubulin isoforms in different tissues of ascaris suum using anti-tubulin monoclonal antibodies.	three monoclonal antibodies specific to alpha- and beta-tubulin were used to examine the expression of tubulin isoforms in the intestine, reproductive tract and body wall muscle of a. suum. the tubulins were found to be different in their isoelectric points, number of isoforms and peptide maps with western blot analysis of one-dimensional polyacrylamide gel confirming the presence of alpha-, beta 1- and beta 2-tubulin. commercial cross-reactive anti-alpha and anti-beta mabs 356 and 357 recognize ...	1991	1787032
[helminth infections in wild boars kept in enclosures in southern germany: severity of infections and fecal intensity].	to determine the intensity of helminth infection and egg output with regard to age and sex of wild boar, stomachs and intestines of 124, lungs of 62, and livers of 39 animals from 5 enclosures in southern germany were examined. the evaluation of the intensity of infection showed 579.4 worms per animal for hyostrongylus rubidus, 510.4 for globocephalus longemucronatus, 476.9 for oesophagostomum dentatum, 476.9 for o. quadrispinulatum, 254.2 for metastrongylus pudendotectus, 176.9 for m. salmi, 14 ...	1991	1796467
airway inflammation in sheep with acute airway hypersensitivity to inhaled ascaris suum.	in order to investigate the relationship between airway inflammation and the development of naturally occurring allergic airway hypersensitivity, the lower respiratory tract was studied in eighteen sheep 14 days after challenge with inhaled ascaris suum antigen. sheep could be classed into three groups on the basis of their response: group a (n = 6) had significant changes in both airway resistance and dynamic lung compliance; group b (n = 6) had changes in only dynamic lung compliance, and grou ...	1991	1804794
[trypsin and alpha-amylase activity in the pancreas of guinea pigs. iv. comparison of the effects of administration of the mixtures of volatile organic acids, invasive ova of ascaris suum and their homogenates].	the activity of alpha-amylase in the experimental groups of guinea pigs was lower than in control animals. the decreased level of activity of trypsin was noted (p less than 0.05) also in infected animals, and in treatment with organic acids. in the group of guinea pigs, which was administrated homogenized eggs of parasite, the activity of enzyme was a little higher than in control animals. the infection of animals was associated with increase of relative weight of lungs. the guinea pigs treatmen ...	1991	1822040
[effects of administration of vitamin a, b 2 and b 15 to guinea pigs during the larval stage of ascariasis on alpha-amylase activity in the lungs and kidneys].	the guinea pigs were administrated vit. a (400 i.u.), vit. b2 (1 mg) or vit. b15 (5 mg). on the 9-th day of the experiment part of them was infected with 5000 invasive eggs of ascaris suum. the invasion lasted 6 days and was controlled by lungs and kidney weight, and number of larvae in the lungs. the activity of amylase was determined by saccharogenic method in both organs. in the lungs of infected animals the activity of alpha-amylase was about 3 times lower than in the control. the infection ...	1991	1822041
[trypsin and alpha-amylase activity in the pancreas of guinea pigs. v. effects of administration of vitamin a and b 2 during the larval stage of ascariasis].	the infection of guinea pigs with ascaris suum larvae resulted in decrease of the activities of trypsin and alpha-amylase, and in increase of lipase activity in extracts from their pancreas. the activity of alpha-amylase, lipase and the relative weight of lungs of infected animals which were given vitamin a, did not differ from control animals. the activity of trypsin from pancreas these animals was higher than that measured in only infected guinea pigs but it was lower than in control animals. ...	1991	1822049
[experimental evaluation of the degree of detectability of helminths' eggs in the soil using 2 methods].	sand and soil were experimentally contaminated with eggs of ascaris suum and oncospheres of hymenolepis diminuta in three doses: 500, 3000 and 5000 eggs (oncospheres) per 50 g of the ground. numbers of recovered eggs were compared with the use of the two following methods: the method of flotation and spindler's method, modified by vasilkova and ivancuk. the ground samples were investigated the following day and on the 14th day after the contamination. the samples were stored in 20 degrees c and ...	1991	1822050
current status of food-borne parasitic zoonoses in singapore.	parasitic infections adopt a rather low profile in the highly urbanized setting in singapore. very few food-borne parasitic infections are encountered. apart from a few reports of infections with clonorchis/opisthorchis, taenia spp. and hydatid disease, there are no other citations of such helminthic infections. seroprevalence surveys have shown the presence of toxoplasmosis in local meat animals (sheep, pigs and cattle) and toxoplasma strains have been isolated from the pig, tree shrew (tupaia ...	1991	1822903
suppression of the ige antibody response by ascaris suum components: effect of x-irradiation.	the effect of x-irradiation on the suppression of ige antibody responses induced by some of the ascaris suum (asc) components was analyzed in mice (7-week old a/sn females). treatment with 300 r 24 h before immunization with 50 micrograms ova and 200 micrograms asc suppressive components abolished the damping effect on anti-ova ige antibody levels. the same effect was observed on the anti-asc ige antibody response obtained in mice injected with 200 micrograms asc immunogenic plus 200 micrograms ...	1991	1823275
effector-induced conformational transitions in ascaris suum phosphofructokinase. a fluorescence and circular dichroism study.	results of activity and spectral studies using fluorescence and circular dichroism show that amp and fructose 2,6-bisphosphate (f-2,6-p2) activate ascaris suum phosphofructokinase through specific and similar conformational changes. inorganic compounds like (nh4)2so4 and kh2po4 also induce structural alterations in the enzyme in a manner different from those caused by amp and f-2,6-p2. the enzyme is activated by both amp and f-2,6-p2, in 20 mm phosphate buffer, ph 6.6, with 0.2 mm atp and 1 mm f ...	1991	1827437
fructose 2,6-bisphosphate and amp increase the affinity of the ascaris suum phosphofructokinase for fructose 6-phosphate in a process separate from the relief of atp inhibition.	kinetic data have been collected suggesting that heterotropic activation by fructose 2,6-bisphosphate and amp is a result not only of the relief of allosteric inhibition by atp but is also the result of an increase in the affinity of phosphofructokinase for fructose 6-phosphate. modification of the ascaris suum phosphofructokinase at the atp inhibitory site produces a form of the enzyme that no longer has hysteretic time courses or homotropic positive (fructose 6-phosphate) cooperativity or subs ...	1991	1827438
trypsin modification of phosphofructokinase from ascaris suum.	phosphofructokinase from ascaris suum is a tetramer with subunits of 90 kda. treatment of the native enzyme with trypsin (10%, w/w) followed by sds-gel electrophoresis was shown to immediately generate a 40-kda fragment followed by a gradual formation of two other fragments of 37 and 32 kda. the loss of catalytic activity during the digestion was less than 50%. gel filtration of the digested enzyme under non-denaturing conditions showed a mr almost that of the native enzyme. digestion of the pho ...	1991	1828862
identification and isolation of a specific antigen with diagnostic potential from dictyocaulus viviparus.	the purpose of the investigation was to isolate and identify a specific antigen of dictyocaulus viviparus that can be used to diagnose lungworm infections in cattle. somatic, excretion and secretion antigens of adult d. viviparus and somatic antigens of l3 larvae were examined in an indirect enzyme-linked immunosorbent assay (elisa) to determine whether they cross-reacted with sera collected from calves with mono-infections of fasciola hepatica. ostertagia ostertagi, ascaris suum, or cooperia on ...	1991	1832795
transmission of intestinal helminths in danish sow herds.	faecal samples from sows and their litters, all untreated with anthelmintics, were examined in eight sow herds, ranging from very intensive to very traditional management. four helminth genera were recorded, namely oesophagostomum sp., ascaris suum, trichuris suis and strongyloides ransomi. the herds with the most intensive management were only infected with a. suum, while the more traditionally managed herds were infected with three or four species. in one herd, the sows showed a small relative ...	1991	1832796
modification of the atp inhibitory site of the ascaris suum phosphofructokinase results in the stabilization of an inactive t state.	treatment of the ascaris suum phosphofructokinase (pfk) with 2',3'-dialdehyde atp (oatp) results in an enzyme form that is inactive. the conformational integrity of the active site, however, is preserved, suggesting that oatp modification locks the pfk into an inactive t state that cannot be activated. a rapid, irreversible first-order inactivation of the pfk is observed in the presence of oatp. the rate of inactivation is saturable and gives a koatp of 1.07 +/- 0.27 mm. complete protection agai ...	1991	1832959
n-acetylglucosaminyltransferase from ascaris suum.	the occurrence of n-acetylglucosaminyltransferase, the initial step in the synthesis of the carbohydrate moiety of n-linked glycoproteins, is demonstrated in the microsomal fraction of the nematode ascaris suum. phosphatidylglycerol stimulated enzyme activity three- to six-fold without affecting the km values of either substrates, uridinediphospho-n-acetylglucosamine or dolichylphosphate. the km values were determined to be about 12 microm and 100 micrograms ml-1, respectively. the enzyme activi ...	1991	1836781
[dissemination and survival of endoparasite embryos in the environment].	pig farms with the annual capacity from five to 20 thousand head represent one of the crucial fields of agriculture influencing the environment in the csfr. daily production of large amounts of liquid excrements (150-300 m3), as a result of high concentrations of animals, poses not only technical but also sanitary problems closely related to the animal and man's health. in a long-term (267 days) field study the authors followed the development and survival of eggs of the model helminth ascaris s ...	1991	1841477
initial characterization of several actin genes in the parasitic nematode, ascaris suum.	southern hybridization data suggest a large actin gene family in ascaris suum. our genomic reconstruction experiment indicated that it consists of 50-75 members. polymorphism was uncovered in the actin genes or in their surrounding sequences. from the genomic library 5 nonoverlapping actin clones were isolated and characterized.	1991	1859418
cut-1 a caenorhabditis elegans gene coding for a dauer-specific noncollagenous component of the cuticle.	we have molecularly identified a new gene of caenorhabditis elegans that codes for a component of the cuticle. the gene has been physically mapped on lgii near the locus sqt-1. the structure and the sequence of the gene have been determined and antisera have been raised against parts of the protein produced as fusions in escherichia coli. by transcription analysis, and by the use of specific antisera, we have determined that this gene is expressed specifically during dauer larva formation. in ex ...	1991	1864469
distribution of 3h-gaba uptake sites in the nematode ascaris.	the distribution of uptake sites for the inhibitory neurotransmitter gamma-aminobutyric acid (gaba) in the nematode ascaris suum was examined by autoradiography of 3h-gaba uptake. single neural processes in both the ventral and dorsal nerve cords were labeled with 3h-gaba. serial section analysis identified the cells of origin of these processes as the rmev-like and rmed-like neurons. these cells belong to a set of four neurons in the nerve ring, all of which are labeled by 3h-gaba. 3h-gaba labe ...	1991	1869634
stimulation of leukocyte lysophospholipase activity by noninfectious agents.	mouse peritoneal leukocyte lysophospholipase (lpl) activity was studied to determine whether or not noninfectious agents cause increased enzyme activity and whether neutrophils have lpl activity. in the first study, mice infected with ascaris suum, a known inducer of lpl activity, were given intraperitoneal injections of proteose peptone, thioglycolate, bovine albumin, paraffin, glycogen, or a. suum whole worm extract (wwe). cell populations collected from mice injected with a. suum wwe, proteos ...	1991	1871154
nonspecific immunomodulation influences resistance of mice to experimental infection with mesocestoides corti and ascaris suum.	the influence of nonspecific immunomodulation on the course of experimental infection was examined in larval cestodosis (mesocestoides corti) and ascaridosis (ascaris suum) in mice. immunosuppressive treatment (with azathioprine or hydrocortisone) resulted in a decrease of resistance in both models. the subsequent administration of t-activin to immunosuppressed mice led to the restoration of resistance to a level equal to that of untreated control mice. the administration of different immunomodu ...	1991	1880386