Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
a preliminary examination of the major integral membrane protein antigens of schistosoma japonicum and schistosoma mansoni adult worms for glycosyl-phosphatidylinositol membrane anchors.integral membrane protein (imp) antigens isolated from s. japonicum and s. mansoni adult worms using triton x-114 phase partitioning were treated with phosphatidylinositol-specific phospholipase c (piplc). following piplc treatment, only one imp antigen of 58 kda from each species was released from the hydrophobic fraction and remained soluble in the absence of detergent. an additional 23 kda antigen was identified following piplc treatment of s. japonicum imp's. this molecule has been previousl ...19911835141
miracidia of schistosoma japonicum: approach and attachment to the snail host.schistosoma japonicum miracidia swim directed along a chemical gradient toward the snails oncomelania hupensis and biomphalaria glabrata, and they turn back when the concentration of attractive chemicals decreases. the host signal for this chemotactic response has a molecular weight of more than 30,000. when swimming miracidia encounter the surface of o. hupensis or agar containing o. hupensis snail-conditioned water (scw) they perform the host-specific responses "contact with return," "repeated ...19911865256
existence of host-related dna sequences in the schistosome genome.dna sequences homologous to the mouse intracisternal a particle and endogenous type c retrovirus were detected in the dnas of schistosoma japonicum adults and s. mansoni eggs. furthermore, other kinds of repetitive sequences in the host genome such as mouse type 1 alu sequence (b1), mouse type 2 alu sequence (b2) and mo-2 sequence, a mouse mini-satellite, were also detected in the dnas from adults and eggs of s. japonicum and eggs of s. mansoni. almost all of the sequences described above were a ...19911866186
identification of a putative eggshell precursor protein of the female schistosoma japonicum.in adult worms of schistosoma japonicum, a prominent radiolabelled female-specific protein (34 kda) was demonstrated on fluorography of sds gels with the pulse incorporation of 14c-tyrosine in vitro, though it was difficult to detect major female-specific proteins by direct staining methods. this female-specific protein was demonstrated to localize exclusively in the vitelline cells by indirect immunofluorescence using the rabbit anti-34 kda female protein antiserum. it was shown that 14c-tyrosi ...19911869356
[anti-antibody responses of rabbit to mouse monoclonal antibody against schistosome "target antigen"].the supernatant of protective mcab (isotype igg2a) secreted by 1e2 hybridoma line was purified by protein a-sepharose cl-4b column and rabbits were immunized with purified 1e2 mcab. dot-elisa, immunodiffusion assay, western blotting and elisa were used to observe the presence of anti-mcab and anti-anti-mcab. the main results are: 1. anti-mcab antibody could be detected as early as 20 days post immunization in the immune sera and would be decreased gradually without boosting; otherwise, it would ...19911873892
quantification of circulating schistosome antigen by monoclonal antibody-based enzyme-linked immunosorbent assay.a two-site enzyme-linked immunosorbent assay (2s-elisa) was developed for the quantification of gut-associated schistosome circulating anodic proteoglycan (gascap) of schistosoma japonicum using a monoclonal antibody (mcab m120), purified by one-step hydroxylapatite (hap) chromatography, as both solid phase and peroxidase-conjugated reagent. in this system, a high detectable level of serum gascap concentration (0.25 microgram/l) could be achieved with the immunoaffinity purified gascap being use ...19911874021
schistosoma haematobium and s. japonicum: analysis of the ribosomal rna genes and determination of the "gap" boundaries and sequences.we have determined the intragenic organization of the rrna genes of schistosoma haematobium and s. japonicum and found them to be similar to that of s. mansoni and other eukaryotes. an entire ribosomal repeat approximately 10 kbp in size from each species was isolated as a sali fragment from a genomic library constructed in bacteriophage lambda. the segments encoding both the small and large rrnas have been identified using three cloned ecori fragments of s. mansoni as probes. there were three e ...19911889469
schistosoma japonicum: changes in lipase activity in adults maintained in vitro.schistosoma japonicum (chinese strain) adult worm pairs (10-12 weeks old) were fixed in 4 degrees c absolute acetone or neutral buffered formalin either immediately after recovery from mice, or following incubation for 0.5 or 1.0 h. males and females were incubated individually in earle's balanced salt solution at 37 degrees c. lipase activity was determined in frozen sections by gomori's method using tween 80 as substrate. in unincubated males, lipase activity was localized only in the parenchy ...19911894435
regulation of the human t-cell response to schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro.serum-mediated regulation of t-cell responses specific for soluble egg antigen (sea) of schistosoma japonicum was tested in human hosts. when we added autologous serum to sea-specific human t-cell lines (cd3+, 4+, 8-), we observed suppression of t-cell proliferation, and this suppressive activity was detected in the immunoglobulin-g2 (igg2) subclass. suppression was dose-dependent and antigen-specific. t-cell proliferation induced by only one sea fraction of greater than 18 kda was modulated in ...19911899724
uptake and effect of praziquantel and the major human oxidative metabolite, 4-hydroxypraziquantel, by schistosoma japonicum.after exposure to praziquantel in vitro at a concentration of 1 microgram/ml for 0.5-2 hr, amounts of praziquantel in schistosoma japonicum varied from 2.1 +/- 1.2 to 3.7 +/- 1.6 ng/male worm and 1.3 +/- 1.2 to 2.2 +/- 1.5 ng/female worm during the time studied. at 30 micrograms/ml, praziquantel amounts were 11-33-fold higher. however, within 2 hr after removal from a medium containing 30 micrograms/ml praziquantel, 95% of the drug was released from the parasites. when s. japonicum worm pairs we ...19911901360
schistosoma japonicum: some parameters affecting the development of protective immunity induced by a cryopreserved, irradiated schistosomula vaccine in guinea-pigs.experiments were conducted in guinea-pigs to elucidate the parameters affecting the development of protective immunity against schistosoma japonicum induced by a cryopreserved, irradiated schistosomula vaccine such as the number of immunizations, route of injection and the use of adjuvants. results obtained indicated that the cryopreserved, irradiated schistosomula vaccine was effective by either intradermal or intramuscular injection. one intradermal injection with bcg adjuvant resulted in an a ...19911903879
activated macrophages in highly irradiated cercariae-induced immunity to schistosoma japonicum in rats.the results of studies on the schistosomulicidal activity of activated peritoneal and alveolar macrophages (pm phi and am phi) from rats immunized with highly irradiated (50 krad.) schistosoma japonicum cercariae are reported. the authors have examined the activation of these macrophages in terms of spreading, adhesion and ingestion of sheep erythrocytes and pinocytosis of horse-radish peroxidase. using three criteria, peritoneal macrophages and alveolar macrophages from immunized rats and from ...19911903881
[demonstration of replication of daughter sporocysts in the natural development of schistosoma japonicum].the participation of replicating sporocysts in the intramolluscal development of schistosoma japonicum within its snail host oncomelania hupensis is demonstrated by histological studies. replicating sporocysts were observed in snails maintained under both standard and hibernation conditions. the demographic and epidemiological importance of the replication process within schistosomes is discussed comparatively.19911904296
[indirect immunoperoxidase staining technique with frozen sections of schistosome for serodiagnosis of latent schistosomiasis].indirect immunoperoxidase staining technique using frozen sections of adult worm as antigen (iip-awa) was carried out to detect antibodies against schistosome antigens (awab) for the diagnosis of existing infection of schistosomiasis in copt positive cases. sera from 229 copt positive and 135 copt negative cases in shanghai county, where schistosomiasis had been eradicated for more than 5 years, were tested. sera from 122 patients with positive stool hatching from an endemic area were served as ...19911905990
infection characteristics of schistosoma japonicum in mice and relevance to the assessment of schistosome vaccines. 19911906234
in vivo regulation of s. japonica granuloma formation by an il-2 antagonist.the present study shows an in vivo role for interleukin-2 in the formation of hepatic granulomatous inflammation in murine schistosoma japonicum infection. mice which had been administered an inhibitor to interleukin-2 function during acute infection were noted to have reduced cell-mediated immune responses to cona and s. japonicum soluble egg antigen (sea). lymphoid tissue from these treated mice also have reduced numbers of cells which could be activated by cona to produce interleukin-2 mrna a ...19911906593
induction of experimental murine granuloma formation against schistosoma japonicum eggs produced by in vitro ova deposition, in vitro tissue extraction, or lyophilization.fresh eggs, laid by schistosoma japonicum adult worms in vitro or recovered from tissue after trypsin digestion, as well as non-viable lyophilized eggs were injected into mice via the cecal vein. the freshly laid eggs induced a marked, maximal reaction at 2 weeks after implantation. this reaction was indistinguishable from that seen early in naturally infected mice. lyophilized eggs induced granulomas only one-tenth the size of those formed around freshly laid eggs. maximal granulomatous reactiv ...19911907742
[localization of antigen and antibodies in the liver and intestine of mice with schistosoma japonicum].indirect fluorescent antibody test was used to localize the antigen in the livers and intestines of mice with schistosoma japonicum. no antigen was detected 4 weeks p.i.. there was a significant rise in antigen level 6 weeks p.i. which reached peak level 10-12 weeks p.i., the gmrt being 512 and 483, respectively. the eggs in the tissues showed strong fluorescent reaction 6 weeks p.i.. the mean diameter of schistosome egg granulomas in the livers and intestines of infected mice was remarkably enl ...19911908356
[observation on the production of interleukin-1 and interleukin-2 and response to interleukin-2 in patients with schistosomiasis].the ability of adherent and nonadherent peripheral blood mononuclear cells (pbmc) from forty patients with schistosomiasis to produce il-1 and il-2 was studied. the results indicated that cona-stimulated nonadherent-pbmc from patients exhibited a decreasing ability to produce il-2 compared with controls (p less than 0.001), and that their cona-activated blast cells expressed a smaller number of cell surface il-2 receptor since il-2 adsorption to the patient cells was lower than that to the norma ...19911908357
[preliminary study of naturally occurring anti-idiotypic antibodies in schistosomiasis japonica].in this study, naturally occurring anti-idiotypic antibodies in sera from patients with schistosomiasis japonica were demonstrated by using mcab (5b5h)-elisa. mcab 5b5h against schistosoma japonicum soluble egg antigen (sea) was purified by precipitation with 50%, 40% and 33% saturated (nh4)2so4 and coupled to cnbr-activated sepharose 4b (pharmacia). 5b5h column was loaded with sera from two patients with schistosomiasis japonica and then the bound material was eluated with 3m kcns. the resultin ...19911908358
recent laboratory investigations by chinese workers on antischistosomal activities of praziquantel. 19911908765
a case of cutaneous squamous cell carcinoma associated with sporadic porphyria cutanea tarda due to liver disorder after schistosoma japonicum infection.a 56-year-old man suffered from cutaneous squamous cell carcinoma (cutaneous scc) which occurred on a cutaneous lesion of sporadic porphyria cutanea tarda (sporadic pct). his liver function decreased from the time he was infected with schistosoma japonicum at the age of 10. he drank a little alcohol. erythematous maculae with blisters or erosions occurred on sun-exposed areas of his skin when he was 52. his urine continued to be red. after detailed examinations including liver biopsy, he was dia ...19911918600
serologic and ultrasonographic parameters of praziquantel treatment of hepatic fibrosis in schistosoma japonicum infection.we describe the parameters useful in evaluating the development of hepatic fibrosis in schistosoma japonicum infection, as well as its improvement after treatment with praziquantel (pzq). various serologic parameters and ultrasonographic images were examined, and their changes were monitored using rabbits infected with 200 or 300 cercariae of s. japonicum. infected rabbits were administered one oral treatment of pzq at a dosage of 100 mg/kg at 6, 12, or 24 weeks after infection. histopathologic ...19911928570
schistosomiasis in singapore--a case report.schistosomiasis although endemic in the far east, has not been reported in singapore. we describe an 83 year-old lady with schistosoma japonicum infection presenting with bloody diarrhoea. we discuss its diagnosis and postulate the mode of transmission of this infection in urban singapore.19911929186
penetration and migration routes of schistosoma japonicum miracidia in the snail oncomelania hupensis.the routes of penetration and the strategies of invasion of schistosoma japonicum miracidia in the snail vector oncomelania hupensis were observed in a histological study. in all species of the genus schistosoma, it is usually assumed that the miracidia achieve penetration through the tegument. our results showed that at least 57% of s. japonicum miracidia penetrated the snail by natural openings (branchial cavity, mouth and rectum). throughout the invasion phase, the larvae were observed in all ...19911945527
bronchoscopic diagnosis of schistosoma japonicum in a patient with hemoptysis. 19911947373
characteristics of different isolates of schistosoma japonicum from china in the final hosts.the present paper deals with the susceptibility of common laboratory animals, such as mouse, rat, hamster, jird, rabbit and rhesus monkey, to infection with different isolates of schistosoma japonicum in the mainland of china under laboratory conditions. with the exception of the rat, all the animals under study were permissive hosts for different isolates though their worm recovery rates varied. the mean body length of pair-worms of the yunnan isolate was considerably smaller than that of the a ...19911948285
compatibility between oncomelania hupensis and different isolates of schistosoma japonicum in china.oncomelania hupensis from six localities were used for infection with different isolates of schistosoma japonicum in the mainland of china, ie anhui in the east, hubei in the center, guangxi in the south, sichuan in the west, yunnan in the southwest and fujian in the southeast. snails from anhui and hubei were readily infected with the local isolate of s. japonicum and cross infection also took place readily between the snails and the schistosomes from these two places. snails from sichuan and y ...19911948286
hepatic schistosomiasis: report of two cases and literature review.two filipino patients were referred for evaluation of abnormal liver enzymes. both patients were known to come from regions endemic for schistosoma japonicum. the clinical laboratory and radiological features of these patients are presented. we highlight the findings of radiologic evaluation, including magnetic resonance imaging (mri). the epidemiology, clinical manifestations, diagnostic strategies including a comparison of the utility of ultrasound, computed tomography, and mri are reviewed. t ...19911951247
hepatic parenchymal dysfunction in schistosoma japonicum infection.decompensated liver disease was investigated in 94 unselected filipino patients sequentially hospitalized with schistosoma japonicum infection. liver damage was objectively scored from 3 (none) to a maximum of 10 (severe damage with encephalopathy) using a modified pugh-child grading system. liver parenchymal dysfunction occurred in 52% of patients and was severe in 21% (hepatic encephalopathy or markedly prolonged prothrombin time). severity scores were higher in patients with previous gastroin ...19911955717
[experimental studies on the concurrent infections with entamoeba histolytica and schistosoma japonicum].the mongolian gerbil (merious unguiculatus) was used as an experimental animal model for studying the concurrent infections and pathogenicities with entamoeba histolytica (e. h.) and schistosoma japonicum (s. j.) by the cutaneous infection with 20 fresh cercariae of s. j. and the intracaecal inoculation of 5 x 10(5)/0.5 ml amebic suspension. light and electron microscopy were used to observe the pathological changes of the caecal wall in the concurrent infections. the results showed that s. j. i ...19911959165
[studies on the strain differences of schistosoma japonicum in the mainland of china. iv. fuzzy cluster analysis on some biological characters of different isolates of schistosoma japonicum].in this paper, a computerized numerical systematics was used to analyse the 5 different isolates of schistosoma japonicum in the mainland of china by means of fuzzy clustering method with 32 quantitative data of each isolate about the morphological and biological characters. a dendrogram of the 5 isolates of s. japonicum was constructed on the basis of similarity coefficients as shown. the phylogenetic relationships revealed by the present study show that the two isolates of anhui and hubei get ...19911959166
[the kinetics of cell-mediated cytotoxicity to schistosomula of schistosoma japonicum with sera from infected mice at different duration].the tests of antibody-dependent cell-mediated cytotoxicity (adcc) were performed to observe the kinetics of in vitro killing of schistosoma japonicum schistosomula mediated by eosinophils, macrophages or neutrophils with infected mouse sera of different duration. the results showed that when complement was not involved, the killing rates of schistosomula mediated by eosinophils or macrophages began to increase significantly at 4 wk post-infection, reached a plateau in 5-7 wk and 6-8 wk respectiv ...19911959170
[establishment and characterization of a monoclonal antibody against egg antigens of schistosoma japonicum].a monoclonal antibody (n29b3) against egg antigens of schistosoma japonicum was obtained by hybridoma technique n29b3 was of igg3 isotype. the molecular weight of its target antigen was 141 kd. iifa with section of liver from infected rabbit showed that n29b3 was located on surface of miracidium and egg shell. iifa and elisa also showed that n29b3 was not reactive with antigens of differential developmental stages of s. japonicum and antigens of other trematodes. the data indicated that n29b3 ha ...19901964846
[detection of a circulating sj 70 antigen in human schistosomiasis japonica using a monoclonal antibody].a double antibody enzyme-linked immunosorbent assay (sandwich elisa) was used for detection of a circulating antigen from human schistosoma japonicum infections. this assay involves the use of poly clonal rabbit anti-schistosoma japonicum soluble egg antigen (sea) antiserum to bind circulating antigen and a monoclonal antibody (mcab-h4) to identify and quantify this antigen. sera from 108 s. japonicum infected patients (acute and chronic) were tested. sera from 93 of 95 (98%) patients with chron ...19901964847
[pathological analysis of lymphonoidi coeliac in patients with schistosomiasis].in specimens from 75 cases of abdominal operation, it was found that ova of schistosoma had deposited in 108 lymphonodi, including 62 lymphoglandulae coelacae and 46 lymphoflandulae mesentericae. quantity of eggs in lymphonodi and 4 conditions of reactions, i.e. sparse distribution, massive lumping, chronic egg-knotting and mixed presence, were studied and analyzed pathologically. the possible ways in which the ova reversely penetrate the lymph nodes through the vein are analyzed and discussed.19901964848
[parasitological and pathological studies on rhesus monkeys infected with chinese mainland strain of schistosoma japonicum].the present study was undertaken to obtain fundamental data from rhesus monkey in the course of infection with chinese mainland strain of schistosoma japonicum under laboratory condition. 15 normal monkeys were infected by cutaneous exposure to 200 cercariae for each animal. the following items were observed: the dynamic distribution of schistosomulum in the skin; the average of worm recovery rate; the size of mature worms of both sexes; the size of mature eggs from the feces; the mean prepatent ...19901964850
[advances in immunologic research in schistosomiasis]. 19901964860
evidence that a 16-kilodalton integral membrane protein antigen from schistosoma japonicum adult worms is a type a2 phospholipase.type a2 phospholipase (pla2) activity has been observed in integral membrane protein extracts of schistosoma japonicum. antiserum raised against bee venom pla2 recognized a single 16-kda band in the parasite extracts; it also localized to antigen in the gut lining of fixed adult schistosomes as shown by immunofluorescence techniques. evidence was obtained that the molecule was expressed at low levels in comparison with other integral membrane proteins and was weakly immunogenic in rabbits. two o ...19912004822
the diagnostic value and molecular characterisation of an echinococcus multilocularis antigen gene clone.a diagnostic echinococcus multilocularis antigen gene (em4) has been expressed using the escherichia coli expression vector pgex-1 resulting in the high level synthesis of a readily purifiable, soluble, non-denatured peptide fused to the 26-kda glutathione-s-transferase of schistosoma japonicum. this recombinant antigen, on testing by enzyme-linked immunosorbent assay (elisa) with heterologous human antisera, demonstrated 100% e. multilocularis specificity. specific anti-em4 antibody immunopreci ...19912011154
[studies on the strain differences of schistosoma japonicum in the mainland of china. iii. morphometric data].the present investigation was undertaken to determine whether differences exist in the size of cercariae, adult worms and eggs among different isolates of s. japonicum in the mainland of china. measurements on the corresponding stages of japanese and philippine strains of s. japonicum were also carried out for comparison. the size of cercariae collected from pooled naturally infected snails from different isolates was shown in table 1. the body index of yunnan isolate, which is defined as cercar ...19912065446
[common antigenic components in soluble antigens shared by schistosoma japonicum eggs and paragonimus westermani adult worms].using enzyme-linked immunoblot technique (elib), the antigenic protein components of water-soluble and urea-soluble antigens of schistosoma japonicum eggs (jsea, jeau) and paragonimus westermani adult worms (paa, paa-u) were analysed. the results showed that in both jsea and jea-u, 8 and 3 bands of polypeptides could be recognized by sera from patients with schistosomiasis japonica (sj). in both paa and paa-u, there were 9 and 2 bands of polypeptides respectively, which could be recognized by se ...19912065450
multi-dot enzyme-linked immunosorbent assay for serodiagnosis of trematodiasis.a nitrocellulose membrane strip dotted with crude antigens of trematodes, such as paragonimus miyazakii, p. westermani, fasciola sp., clonorchis sinensis and schistosoma japonicum was applied for serodiagnosis of trematodiasis. with peroxidase conjugated anti-human igg and 4-chloro-1-naphthol as a substrate, antibodies in trematodiasis patient sera were clearly detected on the strip as bluish purple spots. densities of the spots correlated well with values obtained by standard micro-elisa. cross ...19902075490
[detection of circulating antigen and/or antigen-antibody complex by using mcab against surface membrane antigen of adult schistosoma japonicum].in the present paper, the results of detecting circulating antigen and/or antigen-antibody complexes by mcab against surface membrane antigen of adult schistosoma japonicum were reported. the mcab, coded as 8se4, was prepared by fusion of sp2/0 myeloma cells with spleen cells of the balb/c mice immunized with the saline extract of adult s. japonicum. the 8se4- directed antigen was proved to be located on the surface of the adult worm. after being purified by a de52 column, 8se4 was labelled with ...19902095994
[screening of schistosoma japonicum adult worm genomic dna library by specific antibody].s. japonicum adult worm genomic dna libraries were screened by enzymeimmunoassay. the antigens produced by recombinant lambda gt 11 plaques were transferred to nitrocellulose filters. clones encoding given antigens were detected with infected rabbit sera (irs) which were preabsorbed with lysate of induced lambda gt 11 in y1090 cells. eight putative positives were picked up from 97 plates in the primary screening and were rescreened a second time, and, 2 of them consistently gave good positive si ...19902099255
[studies on the strain differences of schistosoma japonicum in the mainland of china. ii. susceptibility of mammalian hosts].six species of animals were percutaneously exposed to cercariae obtained from pools of naturally infected snails from different isolates of s. japonicum in the mainland of china as shown in tables 1 and 2. our results indicated that with the exception of rat, all animals under study were permissive host though their worm recovery rates varied with different isolates. the mean prepatent period in different host species were 35.0 +/- 0.8 to 36.4 +/- 1.0 days for anhui isolate; 34.5 +/- 1.2 to 36.4 ...19902099256
prospects of vaccination against schistosomiasis.the development of a vaccine against schistosomiasis is necessary in order to reduce the risk of reinfection after drug treatment. recent reports converge on a message strongly supporting the existence of naturally acquired human immunity to this infection and reinforcing the hypothesis that at least partial protection can be achieved by artificial means. advances in molecular biology have led to the identification and characterization of an array of protective schistosome antigens, and the intr ...19902102021
schistosoma japonicum: monoclonal antibodies to the mr 26,000 schistosome glutathione s-transferase (sj26) in an assay for circulating antigen in infected individuals.two monoclonal antibodies have been produced that bind to separate epitopes on the mr 26,000 glutathione s-transferase (gst) of schistosoma japonicum worms (sj26). both antibodies have been used in an enzyme immunoassay (eia) with sera from infected individuals from the philippines. relatively high signals were obtained with sera from some, but not all, individuals who are positive for fecal eggs. evidence was obtained that the material detected by the monoclonal antibodies was present in minute ...19902107094
the effect of praziquantel on the circulating antigen sj 70 in murine schistosomiasis japonica.a circulating schistosome 70 kda antigen (sj 70) has been detected in the sera of mice infected with schistosoma japonicum. for the detection of this antigen, a double antibody sandwich enzyme-linked immunosorbent assay (elisa) was employed. sj 70 is first detected in the serum of s. japonicum-infected mice 4 weeks after infection. antigen levels rise rapidly, plateau by 7 weeks after infection, and remain relatively unchanged for at least another 9 weeks. in mice infected with s. japonicum for ...19902109949
detection of a circulating antigen in human schistosomiasis japonica using a monoclonal antibody.a double antibody enzyme-linked immunosorbent assay (sandwich elisa) was used for the detection of a circulating antigen from human schistosomiasis japonica infections. this assay involves the use of polyclonal rabbit schistosoma japonicum soluble egg antigen (sea) antiserum to bind circulating antigen and a monoclonal antibody (mabh4) to identify and quantify this antigen. sera from 108 s. japonicum-infected patients (acute and chronic) were tested. sera from 93 of 95 patients with chronic infe ...19902109950
antibody is responsible for the passive transfer of immunity to mice from rabbits, rats or mice vaccinated with attenuated schistosoma japonicum cercariae.sera from rabbits, rats and mice multiply-vaccinated with attenuated cercariae of schistosoma japonicum conferred high levels of resistance against challenge to naive recipient mice (up to 97, 64 and 60% respectively). vaccinated rabbit and rat sera were given before challenge and vaccinated mouse serum 5 days after challenge. to show that the protective factors in these sera were antibodies, vaccinated rabbit and mouse sera were fractionated by protein a-sepharose and the fractions precipitated ...19902111906
penetration of schistosoma japonicum cercaria into host skin.the anterior part of schistosoma japonicum cercaria is a specialized head organ which can slightly stretch out and retract. there are three different types of large unicellular glands in cercarial body, consisting of one head gland, 2 pairs of pre- and 3 pairs of postacetabular glands. these glands differ in position, gross feature, histochemistry and functions. both polysaccharase and protease activities are demonstrated in the secretions from these glands. mode of cercarial penetration is desc ...19902112441
growth and fecundity of schistosoma japonicum in mice maintained at different environmental temperatures.on weeks 7 and 9 post infection (pi), the number of schistosoma japonicum in mice maintained at low (5 degrees c) and high (32 degrees c and 35 degrees c) temperatures did not differ from those in controls (25 degrees c), but shorter males were observed in hosts at 5 degrees c. further, both male and female worms recovered on week 7 pi from mice kept at 35 degrees c were shorter than those recovered from controls. staining analysis revealed that worm maturation was not affected in mice kept at 5 ...19902112654
schistosoma japonicum: an ultraviolet-attenuated cercarial vaccine applicable in the field for water buffaloes.water buffaloes were vaccinated three times with 10,000 schistosoma japonicum cercariae irradiated with ultraviolet (uv) light at a dose of 400 microw x min/cm2. the irradiation was performed with cheap, simple, and portable equipment in a rural area of hubei province (people's republic of china). a challenge infection of 1000 untreated cercariae was given to six vaccinated and six naive control buffaloes, while two vaccinated animals were not challenged. the experiment was terminated 6 weeks af ...19902113005
[life span and cercaria shedding of schistosome-infected snails in mountain region of yunnan].the life span and cercaria shedding of infected oncomelania snails in a mountain region of shitoudi village, weishan county, yunnan province were observed in simulated local ecological environments. 135 infected snails were isolated for observation 3 months after exposure to miracidia in august, 1987. the snail survival rate from the day of initial cercaria shedding to next june, july, august and september was 27.4, 16.3, 13.3 and 11.9% respectively, and the average number of cercariae shed was ...19902114229
[snail distribution and susceptible zones of schistosomiasis in endemic areas around poyang lake].to understand the situation of snail distribution and to explore the criteria of susceptible zones of schisosomiasis in endemic areas around poyang lake, a total of 1,222,910 mu of marshland in lake regions were surveyed for three years from 1982 through 1984 by 400 skillful observers. this article reports the relation between mean density of infected snails and incidence of acute schistosomiasis in marshland. we divided all susceptible marshlands of poyang lake area into three types, and found ...19902114231
extramedullary eosinophilopoiesis in the liver of schistosoma japonicum-infected mice, with reference to hemopoietic stem cells.extramedullary hemopoiesis recognized as hemopoietic foci increased in the liver of schistosoma japonicum-infected mice in parallel with kinetic changes in periovular granuloma formation. at the peak of the response, about 65% of the hepatic hemopoietic foci were of eosinophil lineage. when s. japonicum-infected mice were irradiated, hepatic hemopoietic foci rapidly disappeared within 3 days, whereas inflammatory cells in the periovular granulomas slowly reduced in number. when the number of hem ...19902116621
bone marrow and spleen cell colony formation in mice infected with schistosoma japonicum.progenitor cells in the bone marrow and the spleen of mice, whether infected with schistosoma japonicum or not, formed cell clusters and colonies when incubated with culture supernatant fluid of spleen cells incubated with soluble egg antigen (sea). the egg extract, up to a concentration of 250 micrograms/ml protein, did not directly stimulate progenitor cell proliferation in the bone marrow. eosinophilia in mice infected with s. japonicum may be mediated indirectly by egg antigen-stimulated imm ...19902116713
[detection of circulating antigen in the sera in patients with schistosomiasis using monoclonal antibody testing].monoclonal antibody-antigen spot test (mcab-ast) was used for detecting circulating antigen in sera from patients with schistosomiasis japonicum. mcab-ast positive rate in 308 schistosomiasis patients before treatment was 75.97%. among the patients who had moderate or severe infection, the mcab-ast positive rates were 91.18% or 100% respectively. however, the mcab-ast cross-reactive rates in 123 normal donors, 98 clonorchiasis patients, 50 paragonimiasis patients and 56 patients with other paras ...19902119273
[ultrastructural studies on effects of colchicine in treating hepatic fibrosis of schistosomiasis rabbits].8 rabbits were infected percutaneously with the cercariae of schistosoma japonicum. after hepatic fibrosis had developed in these rabbits 4 months after infection, 4 out of the 8 infected rabbits were given colchicine orally at a dosage of 40 micrograms/kg per day for 7 weeks. another uninfected rabbits were used as controls. the therapeutic effects of colchicine on hepatic fibrosis were studied by transmission electron microscopy and morphometry, in which the area of collagen microfibrils in th ...19902119913
schistosoma mansoni and s. japonicum: comparison of levels of ultraviolet irradiation for vaccination of mice with cercariae.when cercariae of schistosoma mansoni and of s. japonicum were irradiated with various levels of u.v. light at 254 nm, their development to perfusable worms was reduced to below 1% at about 200 microw min cm-2. cercariae attenuated with about 300 microw min cm-2 induced partial resistance against an homologous challenge infection in mice. no differences were observed between the two schistosome species when the same treatment was given to the cercariae. thus the same u.v. dose can confer immuniz ...19902122396
studies on the sex ratio of worms in schistosome infections.sex ratios of adult schistosomes in mice are almost invariably different from 1.0 and are biased towards males. the bias applies to wild rats infected with schistosoma japonicum and trapped in an endemic area of the philippines (male:female ratio = 1.7). it also applies to cercariae of snails collected in such areas and assessed by infection of laboratory mice using cercariae from individual snails (male:female ratio may approach 6.0). experiments were designed to determine if duration of infect ...19902122397
importance of mast-cell-derived eosinophil chemotactic factor a on granuloma formation in murine schistosomiasis japonica: evaluation using mast-cell-deficient w/wv mice.the role of mast-cell-derived eosinophil chemotactic factor a (ecf-a) on eosinophil-rich periovular granuloma formation was examined by using mast-cell-deficient wbb6f1-w/wv mice infected with schistosoma japonicum. the average size of granulomas formed around newly deposited eggs in the liver of w/wv mice was significantly smaller than that observed in control +/+ mice. the number of adult worms recovered or specific ige titers were comparable between w/wv and +/+ mice. in contrast, immediate-t ...19902123170
further studies on variable resistance of 129/j and c57bl/6 mice to infection with schistosoma japonicum and schistosoma mansoni.two mouse strains maintained in this laboratory (wehi) are variably resistant to infection with schistosoma japonicum and s. mansoni in that worms cannot be found in the liver and portal system in a high proportion (wehi 129/j mice) or low proportion (c57bl/6 mice) some weeks after exposure to cercariae. resistance can be as high as 100% in wehi 129/j mice and is usually around 20% in c57bl/6 mice. the proportion of resistant mice closely parallels the proportion of mice that demonstrate a shunt ...19902128113
[effect of praziquantel treatment on the levels of circulating sj70 antigen in murine schistosomiasis japonica].a circulating schistosome 70kda antigen (sj 70) has been detected in sera of mice infected with schistosoma japonicum. using a double antibody sandwich enzyme-linked immunoabsorbent assay (elisa). sj 70 antigen was first detected in the sera of s. japonicum-infected mice at the 4th week after infection and increased rapidly, reaching their peak by the 7th week after infection, and remaining relatively unchanged for additional 9 weeks. in contrast, in mice infected with s. japonicum for 7 weeks a ...19902128923
attempts to induce resistance in mice to schistosoma japonicum and schistosoma mansoni by exposure to crude schistosome antigens plus cloned glutathione-s-transferases.several attempts have been made to induce resistance in mice to schistosoma japonicum (philippines) or schistosoma mansoni by exposure to living male and/or female adult worms, their antigens or irradiated cercariae. no resistance was demonstrated in the following cases: re-exposure of mice to cercariae following praziquantel (pzq) treatment of existing infection; re-exposure of mice following cyclosporin a (csa) treatment at the time of first cercarial exposure; subcutaneous or intraperitoneal ...19902129038
[dot-elisa using monoclonal antibody for detecting schistosome circulating antigen].monoclonal antibodies were produced by the fusion of splenic lymphocytes from balb/c mice immunized with schistosoma japonicum excretory/secretory antigen and the myeloma cell line sp2/0. the 1b2e7b8 mcab was proved to be specific against the gut antigen of adult worm in ifa. the mcab labelled with hrp was used in dot-elisa to detect schistosome circulating antigen. schistosome circulating antigen was detected in 152 out of 188 proven cases of schistosomiasis, accounting for a positive rate of 8 ...19902129159
[studies on the dynamic changes in specific antibodies in sera from animals infected with unisexual and bisexual cercariae of schistosoma japonicum].the iest using both liver tissue-egg frozen section of mice infected with schistosoma japonicum and adult worm frozen section of schistosoma japonicum (tefs-iest and awfs-iest) were performed to study the dynamics of specific antibodies in sera from rabbits infected with unisexual and bisexual cercariae of schistosoma japonicum. both peaks of specific antibodies appeared at the 10th week after infection. the levels of specific antibodies were much higher in bisexual infection than in unisexual i ...19902129160
[mcab-elisa for detection of circulating antigen from schistosoma. i. preparation, selection of monoclonal antibody and preliminary testing].fusions between spleen cells from balb/c mice infected with schistosoma japonicum or mansoni cercariae and sp2/0 myeloma were carried out. more than 30 hybridoma cell lines secreting monoclonal antibodies were obtained. their target antigens were integument, gut and egg respectively. the preliminary tests showed that the antibody against gut-associated polysaccharide antigen had the highest activity of all antibodies obtained. this antibody can be used in the sandwich elisa to detect trichloroac ...19902150353
[primary liver cancer coincident with schistosomiasis japonica].the etiologic relationship of a parasitic liver disease to primary liver cancer has long been debated, and reported is a case that has been encountered of a primary liver cancer coincident with schistosomiasis japonica. a fifty-nine year-old-man was diagnosed as having a primary liver cancer complicated with liver cirrhosis. a posterior segmentectomy was performed and a microscopic examination revealed a primary liver cancer with multiple ovae of schistosoma japonicum in the fibrous stroma of th ...19902157081
[detection of circulating antigen in patients with schistosomiasis]. 19902157540
purification of a putative lipoprotein receptor from schistosoma japonicum adult worms.a 43-kda putative lipoprotein receptor from schistosoma japonicum adult worms (sj43) has been purified by reverse-phase high-performance liquid chromatography (hplc) using a waters delta-pak c4 300 a 15 mu 3.9 mm x 300 mm column. a linear acetonitrile gradient from 10-95%, spanning 40 min and at a flow rate of 0.9 ml min-1 was employed for the elution of bound material. sj43 had a retention time of approximately 13 min on the column, whereas other main components from the parasite extract had a ...19902166913
the gene family encoding eggshell proteins of schistosoma japonicum.the four closely related genes encoding eggshell proteins in the human parasite schistosoma japonicum are described. a cdna and a genomic dna library were constructed and members of the eggshell protein gene family isolated. the four genes in this family do not contain introns, and differ in organization and nucleotide sequence from the related set of genes in schistosoma mansoni and schistosoma haematobium. the coding sequences of two of the s. japonicum genes and their flanking regions were de ...19902172818
leishmania major: production of recombinant gp63, its antigenicity and immunogenicity in mice.the mr 63,000 membrane polypeptide (gp63) is one of the leishmania receptors for host macrophages and has been shown to protect mice from infection. the gene encoding gp63, the major mr 63,000 surface glycoprotein of l. major promastigotes, has been expressed as a fusion protein with the enzyme glutathione s- transferase encoded by the parasitic helminth schistosoma japonicum. this fusion protein was recognized by polyclonal antibodies to the native leishmania gp63 polypeptide. the insoluble gp6 ...19902182337
[study on mcabs against protein "target antigen" in schistosoma japonicum].in the studies presented here, we demonstrated the feasibility of producing large number of hybridoma cell lines secreting mcabs against s. japonicum including 16 cell lines secreting igg mcabs (7 for igg1, 5 for igg2a, 1 for igg2b, 3 for igg3) and 13 mcabs for igm, on the basis of successfully extracting 24-26kd and 90kd "target antigen" proteins known as important antigens for inducing schistosome protective immunity. all the above cell lines were characterized for localization of the mcabs, m ...19902208631
[studies on the strain differences of schistosoma japonicum in the mainland of china. i. compatibility between schistosomes and their snail hosts].oncomelania hupensis hupensis from six localities were used in this study, i.e. guichi of anhui in the east, jianli of hubei in the middle, guiping of guangxi in the south, tianquan of sichuan and eryuan of yunnan in the southwest and fuqing of fujian in the southeast. snails from each locality were individually cross-exposed to 20 miracidia of the different isolates of s. japonicum from the above-named places, with the exception of fujian province where no snail could be found naturally infecte ...19902208632
mechanisms of eosinophil accumulation around eggs of schistosoma japonicum: role of two purified components, allergen and eosinophil chemotactic factor, from soluble egg antigens measured on sensitized guinea pig skin.mechanisms of eosinophil response induced by soluble egg antigen (sea) in schistosoma japonicum-infected guinea pig tissues were analyzed using two purified components of sea: major allergenic components (jeal) and a major eosinophil chemotactic component (ecf-sje). eosinophil response to the crude sea of s. japonicum was detected at the injection site mainly at 2-8 h after the antigenic challenge; this response consisted of two different phases of eosinophil attraction. an early phase (2 h) was ...19902217121
production of rat renin fusion protein in escherichia coli and the preparation of renin-specific antisera.rat renin fused at the n-terminus with sj26, a 26,000 da glutathione s-transferase of schistosoma japonicum, was expressed in escherichia coli. the fusion protein was soluble and easily purified from crude bacterial lysates by affinity chromatography on immobilised glutathione. the fusion protein possessed no detectable renin activity. antisera raised in rabbits against the fusion protein were specific for renin. these antisera did not bind soluble renin but bound immobilized renin. by immunoblo ...19902269396
hla-dq-controlled t cell response to soluble egg antigen of schistosoma japonicum in humans.we analysed regulatory mechanisms of the human t cell response to soluble egg antigen (sea) of schistosoma japonicum in vitro. sea is a crude antigen mixture containing numerous epitopes. we obtained sea-induced t cell lines from five patients with chronic schistosomiasis japonica, and tested their proliferative response to molecular weight fractions of sea. although all t cell lines showed strong responses to crude sea, there was a heterogeneity in fraction-driven responsiveness. all but one t ...19902317945
molecular cloning and tissue distribution of a 26-kilodalton schistosoma mansoni glutathione s-transferase.a schistosoma mansoni cdna library was constructed from the mrna of adult worms in the expression vector lambda gt11 and screened with a rabbit antiserum raised against the 26-kda s. mansoni glutathione s-transferase isoforms (sm gst 26). two clones were selected and the nucleotide sequences deduced. the predicted amino acid sequence, specified by these cdnas, shows strong homology with a schistosoma japonicum 26 kda glutathione s-transferase and a lower level of homology with mammalian glutathi ...19902385266
failure to induce resistance of schistosoma japonicum to praziquantel.in order to explore the possible occurrence of inducing resistance of schistosoma japonicum to praziquantel (pzq), a set of animal experiments were carried out. outbred mice (nih strain), anhui isolates of s. japonicum and oncomelania hupensis were used. in one protocol five weeks after being infected with 48-52 cercariae, mice were orally dosed with pzq 300 mg/kg, and killed 82 days later to isolate eggs from the liver. snails were exposed to miracidia released from egg-hatching. f1 progeny wer ...19902402679
characterization of developmentally regulated epitopes of schistosoma mansoni egg glycoprotein antigens.the chemical and antigenic composition of a major group of concanavalin a-binding glycoprotein antigens of schistosoma mansoni eggs was examined by the use of monoclonal antibodies. the individual glycoproteins of this group each displayed a very wide range of apparent m.w. and had isoelectric points of less than 5 when analyzed by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. these glycoproteins could be chemically labeled with 125iodine by two different methods and ...19852409151
stage and sex specific differences in actin gene expression in schistosoma mansoni.we have characterized actin gene expression in schistosoma mansoni at the rna and protein levels. northern blot analyses showed two size classes of actin mrna in eggs, cercariae and adult worms of both sexes, approximately 1 900 and 1 400 bases in length. a higher abundance of actin mrna of both size classes was demonstrated in male worms than in eggs, cercariae, and females. using a phalloidin-rhodamine conjugate, male worms were observed to contain more actin protein than females. southern blo ...19852417116
immunocytochemical localization of gut-associated circulating anodic antigen in schistosoma japonicum.the localization of the gut-associated circulating anodic antigen in schistosoma japonicum adults was revealed by means of the immunofluorescence and a peroxidase-anti-peroxidase method with the electron microscope. the reaction sites were confined to amorphous material in the cecal lumen. the cecal lumen generally was infolded with lamellae. the antigenic material appeared to be secreted by the rough endoplasmic reticulum, probably through the golgi apparatus, into the lumen. observations of ma ...19852417424
molecular identity of a major antigen of schistosoma mansoni which cross-reacts with trichinella spiralis and fasciola hepatica.a glycoprotein cross-reactive among schistosoma mansoni, trichinella spiralis and fasciola hepatica was identified and characterized by use of monoclonal antibodies prepared against s. mansoni glycoproteins. four monoclonal antibodies recognized the same antigen which was one of the major s. mansoni glycoproteins precipitated by sera of hosts infected with either s. mansoni or t. spiralis. this antigen was expressed in s. mansoni cercariae, adult male and female worms, and eggs, and in s. haemat ...19862421229
decalcification of schistosome eggs during staining of tissue sections: a potential source of diagnostic error.miracidial nuclei within calcified schistosome eggs are frequently mummified. calcified eggs are often decalcified prior to or during the routine processing and staining of tissues, and mummified miracidial nuclei then stain clearly and are often arranged in the patterns seen in live eggs. these miracidia may be incorrectly assumed to be alive or to have died recently and thus be taken as evidence of an active infection requiring chemotherapy. the present observations demonstrate the decalcifica ...19862429569
[experimental observations on the efficacy of specific transfer factor and immuno-ribonucleic acid against schistosome infection]. 19862433072
antigenic similarity of onchocerca volvulus to other helminths examined by monoclonal antibodies against o. volvulus.antigens in onchocerca volvulus were characterized using anti-o. vovulus monoclonal antibodies (mcab). a total of 17 mcab which were not species-specific to o. volvulus was examined for the antigenic similarity of o. volvulus with 12 parasitic helminths using indirect immunofluorescence. the range of reactivity of these mcab was wider in nematodes in contrast to species of cestoda or trematoda. the antigenic similarity within helminths examined was generally in agreement with their taxonomic rel ...19862434696
schistosoma japonicum egg antigen-specific t cell lines in man. induction of helper and suppressor t cell lines and clones in vitro in a patient with chronic schistosomiasis japonica.t cell lines (tcls) specific for schistosoma japonicum egg antigen were established from a patient with chronic schistosomiasis japonica to investigate the regulatory mechanism of s.japonicum egg antigen-driven t cell responses in man. all five tcls tested were cd2+, cd4+, cd8-, and were strongly proliferative only to s. japonicum egg antigen in the absence of exogenous il-2. all but one tcl produced il-2-like lymphokines in vitro, indicating their helper t cell functions. one tcl, sje-3, failed ...19882449460
responses in mice to sj26, a glutathione s-transferase of schistosoma japonicum worms.the genetic variation in antibody responses of mice to glutathione s-transferase (gst) enzymes of schistosoma japonicum worms, and in particular to a mr 26,000 species termed sj26, was analysed. sera from infected mice, or mice immunized with adjuvant and an sj26 beta-galactosidase fusion protein produced in escherichia coli (sj26fp), or purified near-native recombinant sj26 produced in e. coli (rsj26), were assayed by enzyme-linked immunosorbent assay (elisa) for antibody titres to gst purified ...19872452132
schistosome heat-shock proteins are immunologically distinct host-like antigens.constitutively expressed schistosome homologues of heat-shock protein hsp70 elicit a dominant antibody response in humans infected with either schistosoma japonicum or schistosoma mansoni; in each case the parasite antigens are immunologically distinct and noncrossreactive. the antigenic site of the homologues is located near their carboxyl terminus where phylogenetic divergence between hsp70 proteins is greatest. nucleotide sequence comparison between these regions predicts very few amino acid ...19882457805
generation and functional characterization of t cell lines and clones specific for schistosoma japonicum egg antigen in humans.t cell lines specific for schistosoma japonicum egg ag were established in vitro from patients with chronic schistosomiasis japonica, and investigated their possible immunopathologic roles by testing lymphokines production and in vitro granuloma formation assay. all lines tested had surface phenotypes of cd3+ cd4+ cd8-, and showed s. japonicum soluble egg ag (sea)-specific proliferation requiring hla-dr-restricted ag presentation. of these fractions of sea separated by gel filtration, fraction i ...19882459210
fl-160. a surface antigen of trypanosoma cruzi that mimics mammalian nervous tissue.chagas' disease, caused by trypanosoma cruzi, is an excellent model for autoimmune disease induced by an infectious agent. transfer of t cells, directed against crossreactive antigens of t. cruzi and nervous tissue, have been shown to reproduce pathology found in chronic chagas' disease. we used recombinant dna technology to characterize one of these crossreactive antigens (fl-160). we have cloned dna from t. cruzi, which expresses a protein corresponding to a 160-kd protein found on the surface ...19892466939
monoclonal antibodies reacting with schistosoma japonicum eggs and their target epitopes.ten monoclonal antibodies (mcabs) raised to schistosoma japonicum eggs could be assigned using several serological and immunochemical techniques to 3 groups. the mcabs, termed a, b and c-mcabs, apparently recognize carbohydrate epitopes that can be located on the same antigen molecule. the antibodies, generally of igm isotype, are idiotypically related. they are distinct from another igm mcab (group d-mcab) the carbohydrate target epitope of which can also be associated with the epitopes of a, b ...19892468264
reactivity of anti-tegument monoclonal antibodies with target epitopes in different worm tissues and developmental stages of schistosoma mansoni.sixteen monoclonal antibodies (mabs) were selected for their reactivity with adult schistosome tegument. the distribution of target epitopes in different tissues of the adult and in various developmental stages was investigated by indirect immunofluorescence. the distinct patterns of reactivity of the mabs permitted their classification into 9 groups. the distribution of epitopes in larvae, particularly 3 h schistosomula, generally mirrored that in adults. a change in distribution of epitopes co ...19892474789
granuloma formation in schistosoma japonicum infected nude mice: the effects of reconstitution with l3t4+ or lyt2+ splenic cells.the role of l3t4+ and lyt2+ lymphocytes in the formation and modulation of granulomas around schistosoma japonicum eggs was examined in athymic mice. nude c57bl/6 mice infected with s. japonicum miracidia were compared to nude mice given normal spleen cells depleted of l3t4+ or lyt2+ cells prior to infection and to intact mice. nude mice formed small granulomas that were poor in eosinophils and connective tissue. nude mice reconstituted with cells enriched for l3t4+ or lyt2+ cells formed granulo ...19892492779
[schistosomiasis].schistosomiasis ranks among the major public health problems in the tropics and subtropics. the disease is caused by the parasite schistosoma, a flatworm with a life cyclus including man as the definitive host and a freshwater snail as the intermediate host. during the last ten years one hundred cases of this disease have been reported in norway. we report two cases of schistosomiasis, one patient infected with schistosoma japonicum, the first reported case in norway, the other with schistosoma ...19892493686
schistosome vaccines: current progress and future prospects.vaccination against human schistosomes in laboratory hosts is now a reality. a number of different parasite molecules have been shown to confer partial protective immunity against challenge infection with schistosoma mansoni or schistosoma japonicum in rodent or primate hosts. these antigens are unusually diverse in their structure and stage specificity. interestingly, although all of the vaccine molecules characterized are situated in the tegument, their exposure on the parasite surface, in mos ...19892498828
evidence of triple infections with schistosoma japonicum, s. haematobium, and s. mansoni in two siblings. 19892499639
persistence of hepatic fibrosis and tissue eggs following treatment of schistosoma japonicum infected mice.the persistence of hepatic fibrosis and of schistosoma japonicum eggs in the tissues of mice was examined after chemotherapy. c57bl/6 mice infected with a philippine strain of s. japonicum were treated with praziquantel or amoscanate 7 or 8 weeks after infection. groups of mice were killed at the time of treatment and 3, 8, 26, and 52 weeks later. the number of eggs per worm pair in the tissues did not change during the year after treatment. however, s. japonicum eggs injected into the tail vein ...19892500856
Refresh
firstprev
12345678910...
nextlast
Displaying items 201 - 300 of 3545