Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| immunological studies in rockland mice infected with t. cruzi. development of antinuclear antibodies. | the existence of antinuclear antibodies (ana) and their relationship with anti-trypanosoma cruzi specific agglutinins was studied in rockland mice inoculated with 10(5), 10(4) or 10(3) epimastigotes and 2 x 10(5) trypomastigotes of t. cruzi. ana and anti-t. cruzi agglutinins were detected at the same time in groups of mice receiving 10(5) culture forms, while in those groups of mice receiving 10(4) parasites agglutinins were established earlier than ana which were present only at low titres. pos ... | 1975 | 131587 |
| [symposium on new approaches in research on american trypanosomiasis]. | 1977 | 142492 | |
| studies on the vesicular component of the auerbach's plexus and the substance p content of the mouse colon in the acute phase of the experimental trypanosoma cruzi infection. | a dramatic reduction in the total number of dense vesicles in auerbach's plexus of the mouse colon was observed during the acute phase of experimental american trypanosomiasis (chagas' disease). a significant decrease in substance p activity of the colon of inoculated animals was also measured. it is suggested that this decrease in substance p activity could be related to the reduction in the number of dense vesicles in auerbach's plexus. | 1977 | 145726 |
| trypanosoma cruzi: isolation and characterization of membrane and flagellar fractions. | 1978 | 153234 | |
| [american trypanosomiasis (chagas' disease) in the caribbean]. | 1978 | 154898 | |
| [domestic triatomines (reduvidae) and the trypanosomic infections in insects in el salvador]. | 1979 | 154908 | |
| [epidemiology of chagas' disease in oaxaca, mexico]. | 1979 | 157759 | |
| [anatomo-pathological study of the brain in idiopathic cardiomegaly]. | the pathological findings in the brain of patients who died with idiopathic cardiomegaly are reported. the major findings in the 38 studied cases were cerebral infarcts (10,5%) and cerebral atrophy (7,9%) besides the nonspecific histological changes represented by neuronal ischemic alteration, satellitosis of nerve cells and swollen and hyperplasia of endothelial cells in capilaries of the cerebral cortex. these findings were considered a consequence of the venous stasis of the congestive heart ... | 1979 | 161163 |
| [evaluation of serological tests used for the study of chagas' disease]. | 2002 | 161709 | |
| adenosine triphosphatase activities in trypanosoma cruzi. | 1. subcellular fractions obtained from epimastigotes of trypanosoma cruzi, disrupted by three different procedures, contained in addition to the already known mg2+-activated adenosine triphosphatase (atpase; e.c.3.6.1.4), a ca2+-atpase activity. 2. the ca2+-atpase (a) was activated by low concentrations of cacl2 (apparent ka, 80 microm); (b) had a km for atp of 0.6 mm (at 1 mm cacl2, ph 8.0); (c) presented a broad ph curve (optimum 7.1-8.6); and (d) was insensitive to oligomycin concentrations w ... | 1978 | 162583 |
| solubilization and some properties of the mg2+-activated adenosine triphosphatase from trypanosoma cruzi. | 1. grinding of epimastigotes of trypanosoma cruzi with glass powder in a mortar yielded a mg2+-activated adenosine triphosphatase (atpase) preparation which was highly sensitive to oligomycin. 2. chloroform treatment of the particles resulted in the solubilization of an atpase which was (a) activated by mgcl2; (b) slightly inhibited by cacl2; (c) activated by sulphite and bisulphite; (d) had an optimum ph of 7.6; and (e) had a km for atp of 2.1 mm (in the presence of 4 mm mgcl2). 3. the solubili ... | 1978 | 162584 |
| isoenzyme patterns of cultured trypanosoma cruzi: changes after prolonged subculture. | 1. the isoenzyme patterns of four soluble enzymes in seven stocks of t. cruzi were determined by electrophoresis. according to their patterns they could be classified into four sets. 2. the isoenzyme patterns of two stocks were influenced by the number of subcultures. 3. five stocks from man are distinct from those derived from a silvatic reservoir. 4. since the isoenzyme patterns of a stock isolated from a patient with acute disease were similar to those of a silvatic reservoir, its recent intr ... | 1979 | 162589 |
| in vitro studies on the differential toxicity of metronidazole in protozoa and mammalian cells. | 1975 | 164840 | |
| immunization against experimental chagas' disease by using culture forms of trypanosoma cruzi killed with a solution of sodium perchlorate. | protection against infection with virulent blood (trypomastigote) forms of trypanosoma cruzi was accomplished in mice by immunization with culture (mainly epimastigote) forms killed by treatment with sodium perchlorate. sodium chloride, used instead of sodium perchlorate, with all other conditions kept the same, failed to kill all the organisms, indicating that the effects of the perchlorate anion were not simply ionic or osmotic, suggesting that they might be chaotropic. a single dose of the im ... | 1975 | 170200 |
| chemotherapeutically active nitro compounds. 4. 5-nitroimidazoles (part i). | more than 135 new 2-methyl-5-nitroimidazoles substituted in 1-position and 1-methyl-5-nitroimidazoles substituted in 2-position were investigated for their activity against various protozoan species, in particular entamoeba histolytica in the golden hamster, trichomonas fetus, trypanosoma brucei and t. cruzi in the nmri mouse. among the nitroimidazoles substituted in the 1-position only two preparations exhibited a similar effect as metronidazole preparations exhibited a similar effect as metron ... | 1977 | 203293 |
| lipid peroxidation and the generation of free radicals, superoxide anion, and hydrogen peroxide in beta-lapachone-treated trypanosoma cruzi epimastigotes. | 1978 | 205176 | |
| isolation of blood and intracellular forms of trypansoma cruzi from rats and other rodents and preliminary studies of their metabolism. | isolation of blood and intracellular forms of trypanosoma cruzi was made mainly from rats (90-110 g) which had received 580 rad of whole-body gamma-irradiation not more than 24 h before subcutaneous inoculation with 10(7) trypomastigotes of the sonya strain of t. cruzi. unirradiated chinchillas (250-350 g) were, however, used for some experiments. blood forms were isolated using a technique involving differential centrifugation to remove most of the erythrocytes and deae-cellulose chromatography ... | 1978 | 206867 |
| immunization of mice against trypanosoma cruzi: the effect of chemical treatment or immune serum on an epimastigote vaccine. | groups of long evans and cd-1 mice were immunized with vaccines prepared from epimastigotes of trypanosoma cruzi, strains y and tulahuen, modified by various chemical means, or by the addition of immune sera. freeze-thawed epimastigotes were used as a control antigen. freeze-thawed epimastigotes, with and without saponin as adjuvant, gave significant protection against challenge with homologous trypomastigotes. similar levels or protection were obtained in mice vaccinated with living epimastigot ... | 1978 | 209592 |
| trypanosoma cruzi: role of the immune response in the natural resistance of inbred strains of mice. | 1978 | 210036 | |
| ultrastructural alterations and peroxide formation induced by naphthoquinones in different stages of trypanosoma cruzi. | addition of beta-lapachone, an o-naphthoquinone with bactericidal, cytotoxic, and trypanocidal activities, to trypanosoma cruzi epimastigote and amastigote stages induced the release of o2- and h2o2 from the whole cells into the suspending medium. in the presence of reduced nicotinamide adenine dinucleotide as reductant beta-lapachone was also able to stimulate o2- and h2o2 production by homogenates of these stages. electron micrographs showed that in beta-lapachone-treated amastigotes and trypo ... | 1978 | 216177 |
| corynebacterium parvum as an adjuvant for trypanosoma cruzi epimastigote vaccines: a comparison with saponin and bordetella pertussis. | the effect was compared in cba mice of adding corynebacterium parvum, saponin, and bordetella pertussis to living or killed trypanosoma cruzi (y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. the addition of c. parvum to a low dose of t. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or b. pertussis. c. parvum alone increased resistance to infection t ... | 1979 | 216967 |
| effect of beta-lapachone on superoxide anion and hydrogen peroxide production in trypanosoma cruzi. | addition of beta-lapachone, an o-naphthoquinone endowed with trypanocidal properties to respiring trypanosoma cruzi epimastigotes induced the release of o2- and h2o2 from the whole cells to the suspending medium. the same beta-lapachone concentration (4 micron) that released h2o2 at maximal rate completely inhibited t. cruzi growth in a liquid medium. the position isomer, alpha-lapachone, did not stimulate o2- and h2o2 release, and did not inhibit epimastigote growth. beta-lapachone was able to ... | 1978 | 217340 |
| conversion of dihydroorotate to orotate in parasitic protozoa. | the conversion of dihydroorotate to orotate, one of the key reactions in the de novo pyrimidine biosynthetic pathway, has been studied in a number of parasitic protozoa. enzyme activities capable of carrying out this reaction were detected in six members of the kinetoplastida (trypanosoma brucei, trypanosoma congolense, trypanosoma vivax, trypanosoma lewisi, trypanosoma cruzi, leishmania enriettii) and three members of the genus plasmodium (p. knowlesi, p. berghei, p. gallinaceum). the mechanism ... | 1979 | 217438 |
| oxidative metabolism in mammalian and culture forms of trypanosoma cruzi. | 1979 | 229013 | |
| [cytochromes in epimastigote form of trypanosoma cruzi]. | 1979 | 231798 | |
| generation of superoxide anion and hydrogen peroxide induced by nifurtimox in trypanosoma cruzi. | 1979 | 232403 | |
| [study of brazilian agricultural workers. a medico-socio-psychological study]. | the general state of health of native brazilian agricultural workers - a total of 750 people from 3 different plantations in the states of paraná and saso paulo - was examined. the main interest of this study war centred on infectious and parasitic diseases, nutritional conditions as well as social, intellectual factors. observations: apart from the high number of cases of helminthiasis, amounting to 80%, the general state of health of the examined subjects was found to be good - indeed better t ... | 1975 | 239549 |
| trypanosoma lewisi, t. acomys and t. cruzi: a method for their cultivation with mammalian tissue. | 1977 | 320031 | |
| inhibition of protein kinase activity from trypanosoma cruzi and trypanosoma gambiense by 3'-deoxyadenosine. | 3'-deoxydadenosine was found to be a potent inhibitor of nucleoside-stimulated protein kinase activity from culture forms of trypanosoma cruzi and bloodstream forms of trypanosoma gambiense. the type of inhibition by 3'-deoxyadenosine was competitive with respect to atp. the inhibition constants for 3'-deoxyadenosine were determined to be 0.11mm and 0.085mm for the enzyme from t. cruzi and t. gambiense, respectively. the apparent km value for atp was 0.2mm for both enzymes. 2'-deoxyadenosine was ... | 1977 | 320116 |
| feeding preferences of triatoma dimidiata maculipennis in yucatan, mexico. | to determine whether a low preference for human blood by triatoma dimidiata maculipennis stal, 1859, the only known vector of chagas' disease in yucatan, mexico, would account for the low prevalence of antibodies to trypanosoma cruzi in man in the area, the intestinal contents of 924 bugs were tested against antisera to blood antigens of chicken, opossum, dog, bat, monkey, pig, goat, rabbit, horse, human, rat, ox, cat, and armadillo. although the chicken was the primary host, man was fed upon fr ... | 1977 | 320892 |
| the effects of lampit (bayer 2502) on the interaction of trypanosoma cruzi with vertebrate cells in vitro. | the effects of 3-methyl-4-(5'-nitrofurfurylidene-amino)-tetrahydro-4h-1,4-thiazine-1,1-dioxide [lampit, bayer 2502] on the intracellular cycle of trypanosoma cruzi were studied with an in vitro steady-state culture system that permitted the continuous analysis of individual host cell-parasite interactions. lampit concentrations of 10(-4) and 10(-5) m significantly affected the ability of trypomastigotes to penetrate vertebrate cells. lampit concentrations above 10(-4) m were toxic to the host ce ... | 1977 | 320896 |
| trypanosoma cruzi: effect of ethidium bromide on growth, dyskinetoplasty, and respiration of the y and costa rica strains. | 1977 | 321238 | |
| biochemical strain characterization of trypanosoma cruzi by restriction endonuclease cleavage of kinetoplast-dna. | 1977 | 321246 | |
| effect of human monocytes and macrophages on trypanosoma cruzi. | studies were undertaken to determine whether trypanosoma cruzi can invade and multiply within human monocytes and macrophages cultured in vitro and, if so, whether macrophages can be activated to inhibit the multiplication. a reticulotropic strain of t. cruzi was capable of infecting human monocytes and monocyte-derived macrophages. intracellular multiplication was observed in both cell types when they were examined microscopically. an increase in the number of trypanosomes occurred in the super ... | 1977 | 321338 |
| establishment and characterization of a cell line (btc-32) from the triatomine bug, triatoma infestans (klug) (hemiptera: reduviidae). | the establishment of a cell line from embryonic tissues of the triatomine bug, triatoma infestans is described. the cell line consists of three cell types which are described, and has a population doubling time of 48 hours during the logarithmic phase of growth. a proportion of the cells remain in the medium as floaters. of the chromosome preparations that could be counted, the majority contained the diploid (2n = 22) number. the cell line has undergone 129 subcultures and has been maintained fo ... | 1977 | 322623 |
| influence of the stage of infection of trypanosoma cruzi in guinea-pigs on infectivity to rhodnius prolixus. | 1977 | 322624 | |
| lipopeptidophosphoglycan from trypanosoma cruzi. amide and ester-linked fatty acids. | lipopeptidophosphoglycan, extracted from whole cells of epimastigote forms of trypanosoma cruzi, has now been shown to contain 12.6% of fatty acids in addition to the previously identified content of neutral sugars (60%), glucosamine (0.8%), peptide (9.5%) and acid-hydrolyzable phosphate (2%). the main fatty acids are palmitic (6.9%) and lignoceric (4.6%) acids. stearic (0.55%), oleic (0.15%) and myristic (0.18%) acids were also found. one third of the fatty acids are bound in the lipopeptidopho ... | 1977 | 323009 |
| [agglutination reaction of t. cruzi, t. cruzi like strains, t. rangeli and t. conorhini with soja hispida lectin and aaptos papillata protectin (author's transl)]. | protectin from the sponge aaptos papillata (keller) was used in the characterization of five strains of t. cruzi (venezuela, guatemala, y. brasilien, peru, wien) and six t. cruzi like strains (triatoma, maryland, itmap 943, fh4, fh5, ln). based upon their membrane receptors, these t. cruzi and t. cruzi like isolates could be differentiated from rangeli (venezuela strain) and t. conorhini (hawai strain) by agglutination reaction to the proctectin. furthermore, after pronase treatment t.rangeli co ... | 1977 | 324052 |
| attempts to define a medium for the in vitro cultivation of trypanosoma cruzi. | 1977 | 324409 | |
| 5-fluorocytosine, a valuable fungicidal agent in specific culture media. | 1977 | 324410 | |
| heterogeneity of the kinetoplast dna molecules of trypanosoma cruzi. | kinetoplast dna (kdna) of the culture form of trypanosoma cruzi is cleaved by restriction endonucleases (hpaii, hindii, ecori, and haeiii). the analysis of the cleavage patterns proves that the minicircles (free circulargenome units) are heterogeneous in base sequences. the same results are obtained with the complex kdna network which is composed of the association of minicircles and linear molecules. kinetic studies of the renaturation of kdna previously cleaved by hpaii into fragments of the g ... | 1977 | 324518 |
| trypanosoma cruzi: ultrastructural and metabolic alterations of epimastigotes by beta-lapachone. | 1977 | 324785 | |
| complement receptors and cell associated complement components. | membrane receptors for activated complement components are widely distributed amongst tissue cells of most mammalian species. common amongst these are receptors for c3b which mediate many of the biological functions of c3. in addition, the genetic control of certain complement components is linked to the genes which code for the major histocompatibility complex. many of these components are also present on cell surfaces. this suggests that the function of the complement system and the major hist ... | 1977 | 324894 |
| intraspecific variation in trypanosoma cruzi: effect of temperature on the intracellular differentiation in tissue culture. | inhibition of t. cruzi amastigote-trypomastigote differentiation in tissue culture at 37 c is a strain-dependent event. when eight t. cruzi strains were submitted to two environmental temperatures (33 and 37 c), the following patterns of differentiation were obtained: in three strains, transformation was inhibited at 37 c but readily occurred at 33 c; in three other strains differentiation took place at both temperatures; finally, in the two remaining strains, a partial inhibition was detected a ... | 1977 | 325187 |
| growth of the y strain of trypanosoma cruzi in an hx25-modified defined medium. | 1977 | 325189 | |
| trypanosoma cruzi: modification of macrophage function during infection. | infection of mice with trypanosoma cruzi and subsequent intraperitoneal challenge with heat-killed trypanosomes elicits peritoneal macrophages which display in vitro microbicidal activity against trypomastigotes of t. cruzi. these cells also display other activated properties including rapid spreading, intense membrane activity, secretion of high levels of plasminogen activator, and ingestion mediated by the c3 receptor. an intravenous infection with bcg, followed by an intraperitoneal challenge ... | 1977 | 327012 |
| trypanosoma cruzi: the immunological induction of macrophage plasminogen activator requires thymus-derived lymphocytes. | in this article we describe methods in which unstimulated mouse peritoneal macrophages were induced to secrete high livels of plasminogen activator under in vitro conditions. the exposure of sensitized peritoneal or spleen cell populations from trypanosoma cruzi-infected animals to either viable or heat-killed trypanosomes lead to the release of an inducing factor(s). maximal levels of plasminogen activator secretion are achieved by the incubation of such factors (s) with unstimulated macrophage ... | 1977 | 327013 |
| catabolism of deoxythymidylate in some trypanosomatids. | an initial observation concerning the failure of [3h]thymidine at high specific activity to be incorporated into the dna of crithidia fasciculata for more than a brief initial period has been correlated with the presence at high specific activity in the organism of a thymidine phosphorylase activity with an equilibrium in the direction of catabolism. this enzyme degrades thymidine to thymine which is not utilized by the organism. the enzyme has also been shown to be present in a number of other ... | 1977 | 327402 |
| geographical extension in a new ecological association of panstrongylus humeralis (hemiptera: reduviidae) natural host of trypanosoma cruzi in panama. | 1977 | 328884 | |
| comparative drug sensitivities of culture forms of trypanosoma cruzi and trypanosoma dionisii. | 1977 | 329144 | |
| eosinophils and not lymphoid k cells kill trypanosoma cruzi epimastigotes. | 1977 | 329145 | |
| some observations on the effect of ro 7-1051 on trypanosoma cruzi, particularly in cell culture. | 1977 | 330210 | |
| trypanosoma cruzi. surface change characteristics of cultured epimastigotes, trypomastigotes and amastigotes. | 1977 | 331436 | |
| [effect of parasite density of ooencyrtus trinidadensis (chalcidoidea, encyrtidae), an endophagus parasite of eggs of rhodnius prolixus, vector of chagas' disease in venezuela]. | 1977 | 331437 | |
| effects of 9-hydroxy ellipticine on in vitro transcription of trypanosoma cruzi dnas. | 1977 | 332175 | |
| fine structure of the trypomastigote form of trypanosoma cruzi isolated from acellular culture by passage in column. | 1977 | 333521 | |
| the citrate synthase from trypanosoma cruzi. | 1977 | 335036 | |
| comparative stability of epimastigotes and trypomastigotes in different wash solutions. | 1977 | 335037 | |
| surface charge of trypanosoma cruzi. binding of cationized ferritin and measurement of cellular electrophoretic mobility. | the surface charge of epimastigote and trypomastigote forms of trypanosoma cruzi was evaluated by means of binding of cationized ferritin to the cell surface as visualized by electron microscopy, and by direct measurements of the cellular microelectrophoretic mobility (epm). epimastigote forms had a mean epm of -0.52 micrometer-s-1-v-1-cm and were lightly labeled with cationized ferritin. in contrast, bloodstream trypomastigotes had a much higher epm (-1.14), and the surface was heavily labeled ... | 1977 | 335054 |
| [effect of beta and alpha-lapachone on the production of h202 and on the growth of trypanosoma cruzi]. | addition of beta-lapachone to the epimastigote (culture) form of trypanosoma cruzi, suspended in saline, buffered-isotonic medium (ph 7.2), determined the appearance of large amounts of h2o2 in the suspension medium, as measured spectrophotometrically by formation of the h2o2 horse radish peroxidase complex. under similar conditions, alpha-lapachone did not induce h2o2 formmation. using nadh as electron donor, beta-lapachone (not alpha-lapachone) increased significantly the rate of h2o2 generat ... | 1977 | 339293 |
| a comparative study of the behavior of venezuelan and brazilian strains of trypanosoma (schizotrypanum) cruzi in the venezuelan invertebrate host (rhodnius prolixus). | 1977 | 339313 | |
| the resistance to human plasma of trypanosoma brucei, t. rhodesiense and t. gambiense: iii. clones of two plasma-resistant strains. | tests for resistance to human plasma were made on six clones of a stabilate of trypanosoma rhodesiense (lump 10) which was calculated to contain about 3,000 resistant trypanosomes per million. two of the clones were not resistant and four were only subresistant. tests were also made on 12 lines (clones) of a stabilate of polymorphic trypanosomes isolated from tsetse flies. one of them, etat 10, had infected a laboratory worker and was found to be fully resistant to human plasma; the other lines ... | 1977 | 339423 |
| growth-inhibition drug test with trypanosoma cruzi culture forms. | a 48-hr drug screening test is described which evaluates inhibition of exponential growth of t. cruzi culture forms by electronic cell count. about 80% of drugs active in vivo produced a greater than 50% growth inhibition, whereas among compounds inactive in vivo, only 19.6% induced such inhibition. advantages of this test are low cost, rapid results, small amounts of drugs needed, and feasibility without animal facilities. comparative studies showed that culture forms are not suitable for scree ... | 1977 | 340677 |
| proteolytic activites in cell extracts of trypanosoma cruzi. | cell extracts of culture forms of trypanosoma cruzi are capable of hydrolysing substances belonging to 4 different groups of protease substrates: (a) substrates for trypsin-like enzymes: benzoyl-arginine-p-nitroanilide and benzoylarginine-naphtylamide; (b) substrates for aminopeptidases: leucyl, lysl and glutamyl-beta-naphtylamide; (c) a substrate fochymotrypsin-like enzymes: carbobenzoxy-l-tyrosine-p-nitorphenylester, and (d) a nonspecific substrate for a broad range of proteases: azocasein. so ... | 1977 | 340678 |
| the number of trypomastigotes of trypanosoma cruzi, required to infect rhodnius prolixus. | 1977 | 341267 | |
| trypanosoma cruzi soluble antigen. role in immunofluorescence, complement fixation and inhibition of hemagglutination tests. | 1977 | 341268 | |
| [studies on reservoirs and wild vectors of trypanosoma cruzi. lxvi: evolution cycle of trypanosoma cruzi in hemocel of panstrongylus megistus]. | 1977 | 343195 | |
| [external phenotypic differences between interspecies hybrids and their respective species, vectors of chagas' disease in brazil (triatominae, reduviidae)]. | 1978 | 345369 | |
| observation on the interaction of peritoneal macrophages with trypanosoma cruzi. ii. intracellular fate of bloodstream forms. | 1977 | 345392 | |
| lipopolysaccharide from trypanosoma cruzi. | 1978 | 345535 | |
| protein typing by disc electrophoresis of some species of trypanosomes with special emphasis to trypanosoma cruzi. | proteins extracted from culture forms of t. cruzi, t. cruzi-like strains, t. rangeli, t. conhorrini and t. dionisii were separated by disc electrophoresis. the electrophoretic protein patterns of all strains examined were highly reproducible. the results indicate that each of these strains have their own "fingerprints" and the trypanosoma species could be identified by some typical specific protein bands. however, it was not possible to distinguish between the human strains of t. cruzi and the t ... | 1978 | 347650 |
| cyanide-sensitive and insensitive respiration of trypanosoma cruzi. | 1978 | 350173 | |
| tricarboxylic acid cycle operation at the kinetoplast-mitochondrion complex of trypanosoma cruzi. | 1978 | 350277 | |
| chemotherapy of parasite infections. | although several good antiparasitic agents are available, new drugs are needed for the treatment of diseases such as chloroquine-resistant malaria, chagas' disease, leishmaniasis and filariasis. the 'semirational approach' should be the basis for their synthesis. | 1981 | 351413 |
| antibody dependent cell-mediated cytotoxicity of trypanosoma cruzi: the release of tritium-labelled rna, dna and protein. | the cytotoxicity of normal rat spleen cells to antibody-coated trypanosoma cruzi epimastigotes has been studied by assaying the release of [3h]-labelled macromolecules from the parasites. the release of thymidine (dna) is slower than the release of uridine (rna), suggesting that the nucleus is broken down more slowly than the cytoplasmic membrane. less than 50% of the leucine (protein) is released when the parasites are lysed, whereas uridine (rna) is almost totally released. in practical terms ... | 1978 | 351526 |
| haemoflagellates: commercially available liquid media for rapid cultivation. | the successful cultivation of a variety of haemoflagellates in three different liquid media is reported. these media include medium 199, grace's insect tissue-culture medium and schneider's drosophila medium, each in combination with 30% (v/v) foetal calf serum. these media were used to cultivate old and new world species of visceral and cutaneous human leishmania, as well as leishmania species isolated from sandflies, rodents, and reptiles. four strains of trypanosoma cruzi, an isolate of t. r- ... | 1978 | 351527 |
| separation with cm-cellulose of the trypomastigote form of trypanosoma cruzi from the forms grown in fibroblast cell cultures. | 1977 | 352340 | |
| trypanosoma cruzi: ultrastructural, cytochemical and freeze-fracture studies of protein uptake. | 1978 | 352715 | |
| trypanosoma cruzi: isolation and characterization of a protease. | 1978 | 352716 | |
| trypanosoma cruzi: in vitro induction of macrophage microbicidal activity. | normal, resident and inflammatory mouse peritoneal macrophages can be induced to display microbicidal activity against trypomastigotes of trypanosoma cruzi by exposure to products from antigen-pulsed, sensitized spleen cell populations. optimal macrophage microbicidal activity was achieved by constant exposure and daily renewal of the spleen cell factors. macrophages obtained after an intraperitoneal injection of mild inflammatory agents were rapidly induced, displaying trypanocidal activity 24 ... | 1978 | 353218 |
| [reservoirs and wild vectors of trypanosoma cruzi. lxvii: various aspects of the behavior of different t. cruzi strains in the coelomic cavity of panstrongylus megistus]. | 1978 | 353908 | |
| blood cultures of trypanosoma cruzi using vacuum culture tubes. | 1978 | 354116 | |
| biochemical characterization of some species of trypansoma (schizotrypanum) from bats (microchiroptera). | trypanosoma (schizotryanum) species isolated from bats (microchirotera) in europe and latin america were examined by determining the buoyant densities of their nuclear and kinetoplastic deoxyribonucleic acid (dna) and the electrophoretic patterns of six isoenzymes. by these criteria they were separated into three distinct groups -- two from europe (t. dionisii and t. vespertilionis) and one from america. t. dionisii was also separable by its morphology in vitro. geographical location and dna buo ... | 1978 | 354417 |
| trypanosoma cruzi: location of a specific antigen on the surface of bloodstream trypomastigote and culture epimastigote forms. | 1978 | 354954 | |
| trypanosoma cruzi: identification of a cell surface polysaccharide. | 1978 | 354955 | |
| trypanosoma cruzi: intracellular stages grown in a cell-free medium at 37 c. | 1978 | 354956 | |
| trypanosoma cruzi: in vitro interactions between cultured amastigotes and human skin-muscle cells. | 1978 | 354958 | |
| [molecular forms of alpha-hydroxyacid dehydrogenase in trypanosoma cruzi]. | 1978 | 355774 | |
| [nucleic acid and proteins content in trypanosoma cruzi epimastigotes]. | the nucleic acids and protein content in epimastigote forms of trypanosoma cruzi during its exponential growth in vitro were studied. the parasites were cultivated in a diphasic blood-agar medium at 28 degrees c. the rna extraction and hydrolisis were performed by the method of fleck and begg and the acid hydrolisis of dna precipitate was done according to webb and lindstrom. schneider's colorimetric method was used for ribose and desoxyribose measurement, employing as reagents orcein and diphen ... | 1978 | 356127 |
| extracellular "in vitro" evolution of metacyclic trypomastigotes isolated from trypanosoma cruzi cultures. | 1978 | 356159 | |
| antitrypanosomal effect of allopurinol: conversion in vivo to aminopyrazolopyrimidine nucleotides by trypanosoma curzi. | the parasite trypanosoma cruzi metabolizes allopurinol by a sequential conversion to allopurinol mononucleotide and aminopurinol mononucleotide. the latter is incorporated into rna. this transformation of a widely used innocuous agent, allopurinol, into a toxic adenine analog appears to account for the antiprotozoan effect of allopurinol. these unique enzymatic activities appear to occur only in t. cruzi and the pathogenic lesihaminae. allopurinol may serve as a model for the synthesis of simila ... | 1978 | 356267 |
| [phospholipids and other lipid components of trypanosoma cruzi. comparative study in tulahuén, es and brazil strains]. | 1978 | 357886 | |
| [hydrogen peroxide metabolism in trypanosoma cruzi]. | 1978 | 357887 | |
| 25 years of neuroepidemiology in the americas. | in the western hemisphere, prior to 1950, there were few reliable data on the incidence, geographic distribution or population patterns of diseases of the nervous system. impressions of prevalence were generally derived from the european literature and from a variety of case reports and relative frequencies based on clinic or hospital admissions or selected autopsy series. since such data from different sources generally defy comparison, it has been necessary to develop data sources for defined ... | 1977 | 358005 |
| [aerobic degradation of glucose by trypanosoma cruzi]. | 1978 | 362118 | |
| [leucine aminotransferase activity in trypanosoma cruzi]. | 1978 | 362119 | |
| the cell surface of trypanosoma cruzi: cytochemistry and freeze-fracture. | the ultrastructure of epimastigotes of trypanosoma cruzi, obtained from acellular cultures, and bloodstream trypomastigotes, isolated from infected mice, were studied by thin-sectioning and freeze-fracturing techniques. epimastigotes showed a thin (5 nm) surface coat when stained with ruthenium red, while the surface coat of trypomastigotes was more prominent (15 nm thick). both p and e faces of the plasma membrane of t. cruzi had roughly the same number of intramembranous particles (imp) as see ... | 1978 | 363731 |
| immunization and enhancement properties of the circulating antigens of trypanosoma cruzi. | 1978 | 364787 | |
| plasma membrane vesicles isolated from epimastigote forms of trypanosoma cruzi. | membrane vesicles can be obtained from epimastigote forms of trypansoma cruzi by incubating cells with either cross-linking reagents or acid ph. acetate, phtalate or citrate, at ph 4.0, but not at higher ph values, were able to induce plasma membrane vesiculation. vesicles have been purified by sucrose density centrifugation and their membrane origin was demonstrated by the following criteria: (a) vesicles are 5--10 times richer in protein-bound iodine when they are prepared from cells previousl ... | 1979 | 365244 |
| purification of an adenylyl cyclase-containing plasma membrane fraction from trypanosoma cruzi. | a fraction containing plasma membrane fragments has been purified from epimastigote forms of trypanosoma cruzi. cells were broken by sonic vibration under well defined conditions and membranes were isolated by differential centrifugation and equilibrium centrifugation in sucrose gradients. the co-purification (approximately 10-fold) of adenylyl cyclase and plasma membrane-bound radioactive iodine is highly suggestive of the localization of this enzyme in the plasma membrane of t. cruzi. determin ... | 1979 | 365245 |