Publications

effects of clbr overexpression on enzyme production in aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species.	clbr is a zn(ii)2cys6 transcriptional activator that controls the expression of cellulase-related genes in response to avicel and cellobiose in aspergillus aculeatus. a clbr-overexpressing strain (clbr-oe) that expresses the clbr gene at levels sevenfold higher than the control strain sustainably produced xylanolytic and cellulolytic activities during 10-day cultivation of a. aculeatus, enabling synchronization of xylanolytic and cellulolytic activities at a maximum level. however, clbr overexpr ...	2015	25410617
identification of cadmium-resistant fungi related to cd transportation in bermudagrass [cynodon dactylon (l.) pers].	phytoremediation utilizing plants and microbes has been increasingly adopted as a green technology for cleaning up heavy metal polluted soils. cd polluted soil and native bermudagrass from liuyang and zhuzhou in hunan province of china were collected to investigate microbial diversity and isolate cd resistant fungi, and then to determine the effect of cd resistant fungi on cd tolerance and transportation of bermudagrass. the functional diversity of microorganisms was evaluated using the biolog e ...	2014	25461949
asperaculanes a and b, two sesquiterpenoids from the fungus aspergillus aculeatus.	six sesquiterpenoids 1-6, including two new ones, an ent-daucane-type sesquiterpenoid, asperaculane a (1), and a nordaucane one, asperaculane b (2), and four known nordaucane derivatives, aculenes a-d 3-6, together with the known secalonic acid d (7), were isolated from a fermentation culture of the fungus aspergillus aculeatus. their structures and absolute configurations were established by analyses of their spectroscopic data, including 1d and 2d-nmr spectra, hr-esims, electronic circular dic ...	2014	25547729
characterization of aspergillus aculeatus β-glucosidase 1 accelerating cellulose hydrolysis with trichoderma cellulase system.	aspergillus aculeatus β-glucosidase 1 (aabgl1), which promotes cellulose hydrolysis by trichoderma cellulase system, was characterized and compared some properties to a commercially supplied orthologue in a. niger (anbgl) to elucidate advantages of recombinant aabgl1 (raabgl1) for synergistic effect on trichoderma enzymes. steady-state kinetic studies revealed that raabgl1 showed high catalytic efficiency towards β-linked glucooligosaccharides. up to a degree of polymerization (dp) 3, raabgl1 pr ...	2015	25642400
biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from penicillium chrysogenum.	rhamnogalacturonan lyase (pcrgl4a) was purified from the culture supernatant of penicillium chrysogenum 31b. pcrgl4a optimal activity occurred between ph 7-8 and at 40 °c. conserved domain search analysis identified pcrgl4a as a member of polysaccharide lyase family 4. pcrgl4a contains two conserved catalytic and four conserved substrate-binding residues as determined by x-ray crystallography of the aspergillus aculeatus rg lyase. recombinant pcrgl4a (rpcrgl4a) expressed in escherichia coli demo ...	2015	25666014
[display cellulolytic enzymes on saccharomyces cerevisiae cell surface by using flo1p as an anchor protein for cellulosic ethanol production].	in this study, we constructed a yeast consortium surface-display expression system by using flo1 as an anchor protein. endoglucanase ii (egii) and cellobiohydrolase ii (cbhii) from trichoderma reesei, and β3-glucosidase 1 (bgli) from aspergillus aculeatus were immobilized on saccharomyces cerevisiae y5. we constructed the cellulose-displaying expression yeast consortium (y5/fegii:y5/fcbhii:y5/fbgli = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. the displayed cellulolyt ...	2014	25720155
crystallization and preliminary x-ray diffraction analysis of an endo-1,4-β-d-glucanase from aspergillus aculeatus f-50.	cellulose is the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in a wide variety of industries. endo-1,4-β-d-glucanase (ec 3.2.1.4; endoglucanase), which can catalyze the random hydrolysis of β-1,4-glycosidic bonds to cleave cellulose into smaller fragments, is a key cellulolytic enzyme. an endoglucanase isolated from aspergillus aculeatus f-50 (fi-cmcase) that was classified into glycoside hydrolase family 12 has been found to be effectively expres ...	2015	25849498
effect of mutations on the thermostability of aspergillus aculeatus β-1,4-galactanase.	new variants of β-1,4-galactanase from the mesophilic organism aspergillus aculeatus were designed using the structure of β-1,4-galactanase from the thermophile organism myceliophthora thermophila as a template. some of the variants were generated using propka 3.0, a validated pka prediction tool, to test its usefulness as an enzyme design tool. the propka designed variants were d182n and s185d/q188t, g104d/a156r. variants y295f and g306a were designed by a consensus approach, as a complementary ...	2015	25941560
dietary sugars analysis: quantification of fructooligossacharides during fermentation by hplc-ri method.	in this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1(f)-fructofuranosylnystose). it was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using aspergillus aculeatus. this analytical technique may be easily implemented at laboratorial or ...	2014	25988114
utilization of recombinant trichoderma reesei expressing aspergillus aculeatus β-glucosidase i (jn11) for a more economical production of ethanol from lignocellulosic biomass.	the capacity of trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant t. reesei strain expressing aspergillus aculeatus β-glucosidase i. we have confirmed highly efficient ethanol production from converge-milled japanese cedar by recombinant t. reesei expressing a. aculeatus β-glucosidase i (jn11). we investigated the ethanol productivity of jn11 and compared it with the cocktail enzyme t. reesei pc-3-7 with reinforced cellobiase a ...	2015	26026380
heterologously expressed aspergillus aculeatus β-glucosidase in saccharomyces cerevisiae is a cost-effective alternative to commercial supplementation of β-glucosidase in industrial ethanol production using trichoderma reesei cellulases.	trichoderma reesei is a filamentous organism that secretes enzymes capable of degrading cellulose to cellobiose. the culture supernatant of t. reesei, however, lacks sufficient activity to convert cellobiose to glucose using β-glucosidase (bgl1). in this study, we identified a bgl (cel3b) from t. reesei (trcel3b) and compared it with the active β-glucosidases from aspergillus aculeatus (aabgl1). aabgl1 showed higher stability and conversion of sugars to ethanol compared to trcel3b, and therefore ...	2016	26073313
imaging of bacterial and fungal cells using fluorescent carbon dots prepared from carica papaya juice.	in this paper, we have described a simple hydrothermal method for preparation of fluorescent carbon dots (c-dots) using carica papaya juice as a precursor. the synthesized c-dots show emission peak at 461 nm with a quantum yield of 7.0 %. the biocompatible nature of c-dots was confirmed by a cytotoxicity assay on e. coli. the c-dots were used as fluorescent probes for imaging of bacterial (bacillus subtilis) and fungal (aspergillus aculeatus) cells and emitted green and red colors under differen ...	2015	26123674
cloning and genomic organization of a rhamnogalacturonase gene from locally isolated strain of aspergillus niger.	the rhg gene encoding a rhamnogalacturonase was isolated from the novel strain a1 of aspergillus niger. it consists of an orf of 1.505 kb encoding a putative protein of 446 amino acids with a predicted molecular mass of 47 kda, belonging to the family 28 of glycosyl hydrolases. the nature and position of amino acids comprising the active site as well as the three-dimensional structure were well conserved between the a. niger ctm10548 and fungal rhamnogalacturonases. the coding region of the rhg ...	2015	26142900
a crispr-cas9 system for genetic engineering of filamentous fungi.	the number of fully sequenced fungal genomes is rapidly increasing. since genetic tools are poorly developed for most filamentous fungi, it is currently difficult to employ genetic engineering for understanding the biology of these fungi and to fully exploit them industrially. for that reason there is a demand for developing versatile methods that can be used to genetically manipulate non-model filamentous fungi. to facilitate this, we have developed a crispr-cas9 based system adapted for use in ...	2015	26177455
rhamnogalacturonan i modifying enzymes: an update.	rhamnogalacturonan i (rgi) modifying enzymes catalyse the degradation of the rgi backbone and encompass enzymes specific for either the α1,2-bond linking galacturonic acid to rhamnose or the α1,4-bond linking rhamnose to galacturonic acid in the rgi backbone. the first microbial enzyme found to be able to catalyse the degradation of the rgi backbone, an endo-hydrolase (ec 3.2.1.171) derived from aspergillus aculeatus, was discovered 25 years ago. today the group of rgi modifying enzymes encompas ...	2016	26255130
investigation of a 6-msa synthase gene cluster in aspergillus aculeatus reveals 6-msa-derived aculinic acid, aculins a-b and epi-aculin a.	aspergillus aculeatus, a filamentous fungus belonging to the aspergillus clade nigri, is an industrial workhorse in enzyme production. recently we reported a number of secondary metabolites from this fungus; however, its genetic potential for the production of secondary metabolites is vast. in this study we identified a 6-methylsalicylic acid (6-msa) synthase from a. aculeatus, and verified its functionality by episomal expression in a. aculeatus and heterologous expression in a. nidulans. feedi ...	2015	26374386
co-expression of endoxylanase and endoglucanase in scheffersomyces stipitis and its application in ethanol production.	scheffersomyces stipitis strain bcc15191 is considered as a biotechnologically valuable yeast for its ability to ferment glucose and xylose, the main sugar components in plant biomass, to ethanol. however, the wild strain lacks of endogenous cellulases and hemicellulases that limited biomass utilization. in order to improve biomass degrading ability of s. stipitis bcc15191, new integrative plasmids harboring constitutive tef1 promoter and codon-optimized zeocin or hygromycin antibiotic resistanc ...	2015	26378014
synergistic action of recombinant accessory hemicellulolytic and pectinolytic enzymes to trichoderma reesei cellulase on rice straw degradation.	synergism between core cellulases and accessory hydrolytic/non-hydrolytic enzymes is the basis of efficient hydrolysis of lignocelluloses. in this study, the synergistic action of three recombinant accessory enzymes, namely gh62 α-l-arabinofuranosidase (ara), ce8 pectin esterase (pet), and gh10 endo-1,4-beta-xylanase (xyl) from aspergillus aculeatus expressed in pichia pastoris to a commercial trichoderma reesei cellulase (accellerase® 1500; acr) on hydrolysis of alkaline pretreated rice straw w ...	2015	26433794
a high performance trichoderma reesei strain that reveals the importance of xylanase iii in cellulosic biomass conversion.	the ability of the trichoderma reesei x3ab1strain enzyme preparations to convert cellulosic biomass into fermentable sugars is enhanced by the replacement of xyn3 by aspergillus aculeatus β-glucosidase 1 gene (aabg1), as shown in our previous study. however, subsequent experiments using t. reesei extracts supplemented with the glycoside hydrolase (gh) family 10 xylanase iii (xyn iii) and gh family 11 xyn ii showed increased conversion of alkaline treated cellulosic biomass, which is rich in xyla ...	2016	26672453
identification and characterization of an acidic and acid-stable endoxyloglucanase from penicillium oxalicum.	xyloglucan is a major structural macromolecule of the primary cell wall of spermatophytes. the hydrolysis of xyloglucan by xyloglucanases may facilitate the hydrolysis of cellulose by cellulases, which is beneficial for bioethanol production. penicillium oxalicum has been employed for commercial cellulase production. in p. oxalicum, many genes and proteins related to the degradation of structural macromolecules of the plant cell wall have been found, but no gene encoding a xyloglucanase has been ...	2016	26840178
identification and functional characterization of a fructooligosaccharides-forming enzyme from aspergillus aculeatus.	although fructosyltransferases from aspergillus aculeatus have received a considerable interest for the prebiotics industry, their amino acid sequences and structural features remain unknown. this study sequenced and characterized a fructosyltransferase from a. aculeatus (acft) isolated by heat treatment of pectinex ultra sp-l. the acft enzyme showed two isoforms, low-glycosylated acft1 and high-glycosylated acft2 forms, with similar optimum activity at 60 °c. the purified heat-resistant acft1 a ...	2016	26857855
towards complete hydrolysis of soy flour carbohydrates by enzyme mixtures for protein enrichment: a modeling approach.	soy protein is a well-known nutritional supplement in proteinaceous food and animal feed. however, soybeans contain complex carbohydrate. selective carbohydrate removal by enzymes could increase the protein content and remove the indigestibility of soy products for inclusion in animal feed. complete hydrolysis of soy flour carbohydrates is challenging due to the presence of proteins and different types of non-structural polysaccharides. this study is designed to guide complex enzyme mixture requ ...	2016	26992789
heterologous expression of aspergillus terreus fructosyltransferase in kluyveromyces lactis.	fructo-oligosaccharides are prebiotic and hypocaloric sweeteners that are usually extracted from chicory. they can also be produced from sucrose using fructosyltransferases, but the only commercial enzyme suitable for this purpose is pectinex ultra, which is produced with aspergillus aculeatus. here we used the yeast kluyveromyces lactis to express a secreted recombinant fructosyltransferase from the inulin-producing fungus aspergillus terreus. a synthetic codon-optimised version of the putative ...	2016	27084521
enhanced cell-surface display and secretory production of cellulolytic enzymes with saccharomyces cerevisiae sed1 signal peptide.	recombinant yeast strains displaying aheterologous cellulolytic enzymes on their cell surfaces using a glycosylphosphatidylinositol (gpi) anchoring system are a promising strategy for bioethanol production from lignocellulosic materials. a crucial step for cell wall localization of the enzymes is the intracellular transport of proteins in yeast cells. therefore, the addition of a highly efficient secretion signal sequence is important to increase the amount of the enzymes on the yeast cell surfa ...	2016	27183011
soybean bio-refinery platform: enzymatic process for production of soy protein concentrate, soy protein isolate and fermentable sugar syrup.	soybean carbohydrate is often found to limit the use of protein in soy flour as food and animal feed due to its indigestibility to monogastric animal. in the current study, an enzymatic process was developed to produce not only soy protein concentrate and soy protein isolate without indigestible carbohydrate but also soluble reducing sugar as potential fermentation feedstock. for increasing protein content in the product and maximizing protein recovery, the process was optimized to include the f ...	2016	27207010
direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts.	laminarin is the algal storage glucan and represents up to 35% of the dry weight of brown macroalgae. in this study, a novel laminarinase, gly5m, was first found using focused proteome analysis of a laminarin-assimilating marine bacterium, saccharophagus degradans, and the encoding gene was isolated. a gly5m-displaying yeast strain was prepared with the cell surface display system using saccharomyces cerevisiae. it showed a laminarin-degrading activity on the cell surface and caused the dominant ...	2016	27287535
site-saturation mutagenesis for β-glucosidase 1 from aspergillus aculeatus to accelerate the saccharification of alkaline-pretreated bagasse.	aspergillus aculeatus β-glucosidase 1 (aabgl1) is one of the best cellobiose hydrolytic enzymes without transglycosylation products, among β-glucosidase from various origins, for use in cellulosic biomass conversion with trichoderma cellulases. however, in our previous report, it was demonstrated that aabgl1 has lower catalytic efficiency toward cellobiose, which is a major end product from cellulosic biomasses by trichoderma reesei cellulases, than do gentiobiose and laminaribiose. thus, we exp ...	2016	27444432
crystal structure and genetic modifications of fi-cmcase from aspergillus aculeatus f-50.	cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. endo-1,4-β-d-glucanase (ec 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-β-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. an endoglucanase isolated from aspergillus aculeatus f-50 (fi-cmcase), which is classified ...	2016	27470581
highly efficient synthesis of fructooligosaccharides by extracellular fructooligosaccharide-producing enzymes and immobilized cells of aspergillus aculeatus m105 and purification and biochemical characterization of a fructosyltransferase from the fungus.	in this work, aspergillus aculeatus m105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. the maximum yields of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. a fructosyltransferase (ftase), aaft32a, was purified from m105. the optimal ph and temperature of aaft32a were ph 5.0-6.0 and 65 °c, respectively. the km, vmax, and kcat values for the transfructosylating activity of a ...	2016	27492129
structure and antitumor activity of the extracellular polysaccharides from aspergillus aculeatus via apoptosis and cell cycle arrest.	two extracellular polysaccharides, designated as wpa and wpb, were isolated from the fungus aspergillus aculeatus using q-sepharose fast flow and sephacryl s-300 column chromatography. wpa composed of mannose and galactose in a molar ratio of 3.9:1.0, and wpb mainly contained mannose. the molecular weight of wpa and wpb was about 28.1 kda and 21.0 kda, respectively. on the basis of methylation and nmr analysis, the possible main chain of wpa was [→5)-β-d-galf-(1 → 2,6)-α-d-manp(1→], and wpb was ...	2016	27557923
screening and identification of novel ochratoxin a-producing fungi from grapes.	ochratoxin a (ota) contamination has been established as a world-wide problem. in this study, the strains with the ability of ota production were screened by analyzing the green fluorescence of the isolates colonies from the grapes in zhenjiang with 365 nm uv light and confirmed by hplc with fluorescent detection (hplc-fld). the results showed that seven isolates acquired the characteristic of the fluorescence, of which only five showed the ability of ota production as confirmed by hplc-fld anal ...	2016	27845758
amelioration of salt stress on bermudagrass by the fungus aspergillus aculeatus.	there is considerable evidence that plant abiotic-stress tolerance can be evoked by the exploitation of a globally abundant microbe. a. aculeatus, which was initially isolated from the rhizosphere of bermudagrass, has been shown to increase heavy metal tolerance in turfgrasses. here, we report on the potential of a. aculeatus to induce tolerance to salt stress in bermudagrass. physiological markers for salt stress, such as plant growth rate, lipid peroxidation, photosynthesis, and ionic homeosta ...	2017	28134574
unravelling the nanostructure of strawberry fruit pectins by endo-polygalacturonase digestion and atomic force microscopy.	pectins analysed by afm are visualized as individual chains, branched or unbranched, and aggregates. to investigate the nature of these structures, sodium carbonate soluble pectins from strawberry fruits were digested with endo-polygalacturonase m2 from aspergillus aculeatus and visualized by afm. a gradual decrease in the length of chains was observed as result of the treatment, reaching a minimum ln value of 22nm. the branches were not visible after 2h of enzymatic incubation. the size of comp ...	0	28159266
production of high galacto-oligosaccharides by pectinex ultra sp-l: optimization of reaction conditions and immobilization on glyoxyl-functionalized silica.	a rational optimization for the synthesis of galacto-oligosaccharides (gos) from lactose catalyzed by β-galactosidase from aspergillus aculeatus, included in the commercial product pectinex ultra sp-l, has been performed by using experimental design and surface response methodology. this accurate tool optimized empirical production of the most desired high-gos (tri-gos and tetra-gos) up to 16.4% under the following reaction conditions: 59 °c, 4 u/ml free enzyme concentration, ph 6.5, 250 g/l ini ...	2017	28176525
heterologous expression of aspergillus aculeatus endo-polygalacturonase in pichia pastoris by high cell density fermentation and its application in textile scouring.	removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. in this study, an endo-polygalacturonase (endopg) from aspergillus aculeatus produced in pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation.	2017	28209146
secalonic acid- f inhibited cell growth more effectively than 5-fluorouracil on hepatocellular carcinoma in vitro and in vivo.	hepatocellular carcinoma (hcc), one of the most common types of liver cancer, could be treated with 5-fluorouracil (5-fu). due to its side effects, 5-fu is more often used as the co-administration drug in clinical practice. secalonic acid-f (saf), isolated from a fungal strain identified in our lab as aspergillus aculeatus, showed potent biological activities. the goal of this study was to evaluate the inhibitory effects of saf on hepatocellular carcinoma and to compare it with that of 5-fu. res ...	2017	28253713
effects of cadmium-resistant fungi aspergillus aculeatus on metabolic profiles of bermudagrass [cynodondactylon (l.)pers.] under cd stress.	plants' tolerance to heavy metal stress may be induced by the exploitation of microbes. the objectives of this study were to investigate the effect of cadmium (cd)-resistant fungus, aspergillus aculeatus, on tolerance to cd and alteration of metabolites in bermudagrass under cd stress, and identify the predominant metabolites associated with cd tolerance. two genotypes of bermudagrass with contrasting cd tolerance (cd-sensitive 'wb92' and cd-tolerant 'wb242') were exposed to 0, 50, 150 and 250 m ...	2017	28273510
dipeptidyl peptidase iv is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in aspergillus aculeatus.	we screened for factors involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes from approximately 12,000 aspergillus aculeatus t-dna insertion mutants harboring a transcriptional fusion between the fiii-avicelase gene (cbhi) promoter and the orotidine 5'-monophosphate decarboxylase gene. analysis of 5-fluoroorodic acid (5-foa) sensitivity, cellulose utilization, and cbhi expression of the mutants revealed that a mutant harboring t-dna at the dipeptidyl peptida ...	2017	28290772
optimization of process parameters for enhanced production of jamun juice using pectinase (aspergillus aculeatus) enzyme and its characterization.	jamun fruit comprises of seed and thick pulp. the pectin-protein bond of the thick pulp creates difficulty in making juice. clear jamun juice is not available in the market, so there is a need for extraction of juice with maximum yield. the goal of this research is to obtain high yield of clarified juice with the help of pectinase (aspergillus aculeatus) enzyme. the study was conducted at different enzyme concentration (0.01-0.1%), time duration (40-120 min), and temperature (30-50 °c). various ...	2016	28330313
metabolomic tools to assess the chemistry and bioactivity of endophytic aspergillus strain.	endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical and agrochemical drugs. in this study, a combination of metabolomics and bioactivity-guided approaches were employed to isolate anticancer secondary metabolites from an endophytic aspergillus aculeatus. the endophyte was isolated from the egyptian medicinal plant terminalia laxiflora and identified using molecular biological methods. metabolomics an ...	2017	28672096
does osmotic stress affect natural product expression in fungi?	the discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. however, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. an unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawa ...	2017	28805714
characterization of mechanisms underlying degradation of sclerotia of sclerotinia sclerotiorum by aspergillus aculeatus asp-4 using a combined qrt-pcr and proteomic approach.	the biological control agent aspergillus aculeatus asp-4 colonizes and degrades sclerotia of sclerotinia sclerotiorum resulting in reduced germination and disease caused by this important plant pathogen. molecular mechanisms of mycoparasites underlying colonization, degradation, and reduction of germination of sclerotia of this and other important plant pathogens remain poorly understood.	2017	28859614
simultaneous saccharification and viscosity reduction of cassava pulp using a multi-component starch- and cell-wall degrading enzyme for bioethanol production.	in this study, an efficient ethanol production process using simultaneous saccharification and viscosity reduction of raw cassava pulp with no prior high temperature pre-gelatinization/liquefaction step was developed using a crude starch- and cell wall-degrading enzyme preparation from aspergillus aculeatus bcc17849. proteomic analysis revealed that the enzyme comprised a complex mixture of endo- and exo-acting amylases, cellulases, xylanases, and pectina ses belonging to various glycosyl hydrol ...	2017	28868217