Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| nanoscale structure of the cell wall protecting cellulose from enzyme attack. | the cell wall structure protects cellulose from enzymatic attack and its successive fermentation. the nature of this protection consists in the very complex macroscopic and microscopic structure of cell wall that limits transport. explaining this kind of protection is critical in future research to improve cell polymer availability for enzymatic attack. this research shows that the complete description of the cell wall topography at a nanoscale level allows a mechanistic understanding of cellulo ... | 2011 | 21174466 |
| identification of candidate genes associated with senescence in durum wheat (triticum turgidum subsp. durum) using cdna-aflp. | senescence is an integrated response of plants to various internal (developmental) and external (environmental) signals. it is a highly regulated process leading eventually to the death of cells, single organs such as leaves, or even whole plants. in cereals, which are monocarpic plants, senescence represents the final stage of development. in order to study senescence in durum wheat (triticum turgidum subsp. durum), a cdna-aflp analysis was performed. the transcription profiles of plants at dif ... | 0 | 21197602 |
| assessment of genetic diversity among syrian durum (triticum turgidum ssp. durum) and bread wheat (triticum aestivum l.) using ssr markers. | genetic diversity among 49 wheat varieties (37 durum and 12 bread wheat) was assayed using 32 microsatellites representing 34 loci covering almost the whole wheat genome. the polymorphic information content (pic) across the tested loci ranged from 0 to 0.88 with average values of 0.57 and 0.65 for durum and bread wheat respectively. b genome had the highest mean number of alleles (10.91) followed by a genome (8.3) whereas d genome had the lowest number (4.73). the correlation between pic and all ... | 2010 | 21254727 |
| meiotic behaviour of tetraploid wheats (triticum turgidum l.) and their synthetic hexaploid wheat derivates influenced by meiotic restitution and heat stress. | meiotic restitution is considered to be a common mechanism of polyploidization in plants and hence is one of the most important processes in plant speciation. meiotic behaviour of plant chromosomes is influenced by both genetic and environmental factors. in this study, the meiotic behaviour of cereal crops was investigated, which includes tetraploid wheat genotypes (with and without the meiotic restitution trait) and their derivates (synthetic hexaploid wheats and a doubled haploid (dh) line), g ... | 2010 | 21273690 |
| the drought response displayed by a dre-binding protein from triticum dicoccoides. | drought is one of the major causes of dramatic yield loss in crop plants. knowledge of how to alleviate this loss is still limited due to the complexity of both the stress condition and plant responses. wild emmer wheat (triticum turgidum ssp. dicoccoides) is a potential source of important drought-resistance genes for its cultivated relatives. the gene for an emmer dre-binding protein, tdicdrf1, was cloned and shown to be drought-responsive with orthologs in other plants. this is the first repo ... | 2011 | 21296583 |
| synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process. | doubled haploid (dh) populations are useful to scientists and breeders in both crop improvement and basic research. current methods of producing dhs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. this report describes a simple method for synthesizing dhs (syndh) especially for allopolyploid species by utilizing meiotic restitution genes. the method involves three steps: hybridization to induce recombination, interspecific hybridization to ex ... | 2011 | 21356528 |
| major genes for na+ exclusion, nax1 and nax2 (wheat hkt1;4 and hkt1;5), decrease na+ accumulation in bread wheat leaves under saline and waterlogged conditions. | two major genes for na(+) exclusion in durum wheat, nax1 and nax2, that were previously identified as the na(+) transporters tmhkt1;4-a2 and tmhkt1;5-a, were transferred into bread wheat in order to increase its capacity to restrict the accumulation of na(+) in leaves. the genes were crossed from tetraploid durum wheat (triticum turgidum ssp. durum) into hexaploid bread wheat (triticum aestivum) by interspecific crossing and marker-assisted selection for hexaploid plants containing one or both g ... | 2011 | 21357768 |
| alteration in expression of hormone-related genes in wild emmer wheat roots associated with drought adaptation mechanisms. | transcriptomic and metabolomic profiles were used to unravel drought adaptation mechanisms in wild emmer wheat (triticum turgidum ssp. dicoccoides), the progenitor of cultivated wheat, by comparing the response to drought stress in roots of genotypes contrasting in drought tolerance. the differences between the drought resistant (r) and drought susceptible (s) genotypes were characterized mainly by shifts in expression of hormone-related genes (e.g., gibberellins, abscisic acid (aba) and auxin), ... | 2011 | 21656015 |
| identification and characterization of two plasma membrane aquaporins in durum wheat (triticum turgidum l. subsp. durum) and their role in abiotic stress tolerance. | plant plasma membrane intrinsic proteins (pip) cluster in two phylogenetic groups, pip1 and pip2 that have different water channel activities when expressed in xenopus oocytes. pip2s induce a marked increase of the membrane osmotic water-permeability coefficient (p(f)), whereas pip1s are generally inactive. here we report the cloning of two durum wheat (triticum turgidum l. subsp. durum) cdnas encoding tdpip1;1 and tdpip2;1 belonging to the pip1 and pip2 subfamilies, respectively. contrary to td ... | 2011 | 21723739 |
| chromosomal and genome-wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental. | genomic instability can be induced by nascent allopolyploidization in plants. however, most previous studies have not defined to what extent the allopolyploidy-induced rapid genomic instability represents a general response, and hence important to evolution, or merely incidental events occurring stochastically in a limited number of individuals. we report here that in a newly formed allohexaploid wheat line between tetraploid wheat triticum turgidum subsp. durum (genome bbaa) and aegilops tausch ... | 2011 | 21797821 |
| environmental conditions affect semolina quality in durum wheat (triticum turgidum ssp. durum l.) cultivars with different gluten strength and gluten protein composition. | sowing time may impact semolina and pasta cooking quality by changing the environmental conditions during grain filling. the effect of an optimum and a delayed sowing time on semolina quality was studied by comparing six cultivars under irrigation, in order to isolate temperature from drought effects. | 2011 | 21842525 |
| variations in content and extractability of durum wheat (triticum turgidum l. var durum) arabinoxylans associated with genetic and environmental factors. | arabinoxylans (ax) represent the most abundant components of non-starch polysaccharides in wheat, constituting about 70% of cell wall polysaccharides. an important property of ax is their ability to form highly viscous water solutions; this peculiarity has a significant impact on the technological characteristics of wheat and determines the physiologically positive influence in consumption. durum wheat (triticum turgidum l. var durum), the raw material for pasta production, is one of the most im ... | 2011 | 21845095 |
| low temperature-induced necrosis shows phenotypic plasticity in wheat triploid hybrids. | hybrid necrosis sometimes appears in triploid hybrids between tetraploid wheat and aegilops tauschii coss. two types of hybrid necrosis (type ii and type iii) were observed when cultivar langdon was used as female parent for hybrid production. type ii necrosis symptoms occurred only under low temperature conditions, whereas bushy and dwarf phenotypes were observed under normal temperature conditions. the developmental plasticity might be related to a temperature-responsive alteration of meristem ... | 2011 | 21897130 |
| disturbance of reactive oxygen species homeostasis induces atypical tubulin polymer formation and affects mitosis in root-tip cells of triticum turgidum and arabidopsis thaliana. | in this study, the effects of disturbance of the reactive oxygen species (ros) homeostasis on the organization of tubulin cytoskeleton in interphase and mitotic root-tip cells of triticum turgidum and arabidopsis thaliana were investigated. reduced ros levels were obtained by treatment with diphenylene iodonium (dpi) and n-acetyl-cysteine, whereas menadione was applied to achieve ros overproduction. both increased and low ros levels induced: (a) macrotubule formation in cells with low ros levels ... | 2012 | 21976360 |
| occurrence of fusarium spp. and fumonisin in durum wheat grains. | a survey was carried out to determine fusarium species and fumonisin contamination in 55 durum wheat (triticum turgidum l. var. durum) samples collected during two harvest seasons (2007 and 2008) using hplc and further lc-ms/ms confirmation. all samples showed fusarium contamination with infection levels ranging from 8 to 66%, f. proliferatum being the species most frequently isolated during 2007 and the second most frequently isolated one during the 2008 harvest season, respectively. natural co ... | 2011 | 21999326 |
| a new reduced height gene found in the tetraploid semi-dwarf wheat landrace aiganfanmai. | aiganfanmai is a dwarf tetraploid wheat landrace (triticum turgidum var. turgidum) that stably produces the semi-dwarf trait. plant height varies from 80-105 cm under cultivation. compared with tall durum wheat (t. turgidum var. durum) variety langdon, we found it to have short spikes and seeds, besides a semi-dwarf character. we crossed aiganfanmai with langdon to analyze the genetic basis of the semi-dwarf trait. the f(2) population segregated at a 1:3 ratio for the short trait to the normal, ... | 2011 | 22002128 |
| genetic behaviour of physiological traits conferring cytosolic k+/na+ homeostasis in wheat. | a plant's ability to maintain an optimal cytosolic k(+)/na(+) ratio has long been cited as a key feature of salinity tolerance. as traditional whole-leaf nutrient analysis does not account for tissue and organelle-specific ion sequestration, the predictive value of this index at the whole-plant level is not always satisfactory. consequently, suitable in situ methods for functionally assessing the activity of the key membrane transporters contributing to this trait at the cellular level need to b ... | 2012 | 22117736 |
| identification and characterization of a novel powdery mildew resistance gene pmg3m derived from wild emmer wheat, triticum dicoccoides. | powdery mildew, caused by blumeria graminis f. sp. tritici (bgt) is one of the most important wheat diseases worldwide. wild emmer wheat, triticum turgidum ssp. dicoccoides, the tetraploid ancestor (aabb) of domesticated bread and durum wheat, harbors many important alleles for resistance to various diseases, including powdery mildew. in the current study, two tetraploid wheat mapping populations, derived from a cross between durum wheat (cv. langdon) and wild emmer wheat (accession g-305-3m), w ... | 2011 | 22159825 |
| identification and comparative mapping of a powdery mildew resistance gene derived from wild emmer (triticum turgidum var. dicoccoides) on chromosome 2bs. | powdery mildew, caused by blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. wild emmer (triticum turgidum var. dicoccoides) is a valuable genetic resource for improving disease resistance in common wheat. a powdery mildew resistance gene conferring resistance to b. graminis f. sp. tritici isolate e09 at the seedling and adult stages was identified in wild emmer accession iw170 introduced from israel. an incomplete dominant gene, temporarily designated mliw170, ... | 2011 | 22170431 |
| immunogenicity characterization of two ancient wheat α-gliadin peptides related to coeliac disease. | the immunogenic potential of α-gliadin protein from two ancient wheats was studied with reference to coeliac disease. to this aim we investigated graziella ra® and kamut® (the latter is considered an ancient relative of modern durum wheat) in comparison to four durum wheat accessions (senatore cappelli, flaminio, grazia and svevo). elisa and western blot analyses - carried out by two monoclonal antibodies raised against the α-gliadin peptides p31-49 (lgqqqpfpqqpypqpqpf) and p56-75 (lqlqpfpqpqlpy ... | 2009 | 22253984 |
| global transgenerational gene expression dynamics in two newly synthesized allohexaploid wheat (triticum aestivum) lines. | alteration in gene expression resulting from allopolyploidization is a prominent feature in plants, but its spectrum and extent are not fully known. common wheat (triticum aestivum) was formed via allohexaploidization about 10,000 years ago, and became the most important crop plant. to gain further insights into the genome-wide transcriptional dynamics associated with the onset of common wheat formation, we conducted microarray-based genome-wide gene expression analysis on two newly synthesized ... | 2012 | 22277161 |
| effect of silicon on reducing cadmium toxicity in durum wheat (triticum turgidum l. cv. claudio w.) grown in a soil with aged contamination. | agricultural soil contamination and subsequently crops still require alternative solutions to reduce associated environmental risks. the effects of silica application on alleviating cadmium (cd) phytotoxicity in wheat plants were investigated in a 71-day pot experiment conducted with a historically contaminated agricultural soil. we used amorphous silica (asi) that had been extracted from a diatomite mine for si distribution at 0, 1, 10 and 15 ton asi ha(-1). asi applications increased plant bio ... | 2012 | 22301080 |
| high-density mapping and marker development for the powdery mildew resistance gene pmas846 derived from wild emmer wheat (triticum turgidum var. dicoccoides). | powdery mildew, caused by blumeria graminis f. sp. tritici, is an important foliar disease of wheat worldwide. the dominant powdery mildew resistance gene pmas846 was transferred to the hexaploid wheat lines n9134 and n9738 from wild emmer wheat (triticum dicoccoides) in 1995, and it is still one of the most effective resistance genes in china. a high resolution genetic map for pmas846 locus was constructed using two f(2) populations and corresponding f(2:3) families developed from the crosses o ... | 2012 | 22350087 |
| genetic variability in arbuscular mycorrhizal fungi compatibility supports the selection of durum wheat genotypes for enhancing soil ecological services and cropping systems in canada. | crop nutrient- and water-use efficiency could be improved by using crop varieties highly compatible with arbuscular mycorrhizal fungi (amf). two greenhouse experiments demonstrated the presence of genetic variability for this trait in modern durum wheat ( triticum turgidum l. var. durum desf.) germplasm. among the five cultivars tested, 'ac morse' had consistently low levels of am root colonization and dt710 had consistently high levels of am root colonization, whereas 'commander', which had the ... | 2012 | 22356605 |
| formation of an endoplasmic reticulum ring associated with acetylated microtubules in the angiosperm preprophase band. | we investigated the organization of the cortical endoplasmic reticulum (er) in prophase cells of the angiosperms zea mays, triticum turgidum, and vigna sinensis. in both symmetrically and asymmetrically dividing protodermal leaf cells, cortical er was enriched in the preprophase band and colocalized there with microtubules, forming a ring-like structure (er ring). in contrast, er ring was absent from prophase root-tip cells of the same plants, suggesting that er ring formation in the preprophase ... | 2012 | 22359412 |
| mixed model association mapping for fusarium head blight resistance in tunisian-derived durum wheat populations. | sources of resistance to fusarium head blight (fhb) in wheat are mostly restricted to chinese hexaploid genotypes. the effort to incorporate the resistance from hexaploid wheat or wild relatives to cultivated durum wheat (triticum turgidum l. var. durum desf.) have not been successful in providing resistance to the level of the donor parents. in this study, we used 171 bc(1)f(6) and 169 bc(1)f(7) lines derived from crossing of four tunisian tetraploid sources of resistance (tun7, tun18, tun34, t ... | 2011 | 22384332 |
| a novel retrotransposon inserted in the dominant vrn-b1 allele confers spring growth habit in tetraploid wheat (triticum turgidum l.). | vernalization genes determine winter/spring growth habit in temperate cereals and play important roles in plant development and environmental adaptation. in wheat (triticum l. sp.), it was previously shown that allelic variation in the vernalization gene vrn1 was due to deletions or insertions either in the promoter or in the first intron. here, we report a novel vrn-b1 allele that has a retrotransposon in its promoter conferring spring growth habit. the vrn-b1 gene was mapped in a doubled haplo ... | 2011 | 22384375 |
| wheat grain yield on saline soils is improved by an ancestral na⁺ transporter gene. | the ability of wheat to maintain a low sodium concentration ([na(+)]) in leaves correlates with improved growth under saline conditions. this trait, termed na(+) exclusion, contributes to the greater salt tolerance of bread wheat relative to durum wheat. to improve the salt tolerance of durum wheat, we explored natural diversity in shoot na(+) exclusion within ancestral wheat germplasm. previously, we showed that crossing of nax2, a gene locus in the wheat relative triticum monococcum into a com ... | 2012 | 22407351 |
| identification of snp mutations in dreb1, hkt1, and wrky1 genes involved in drought and salt stress tolerance in durum wheat (triticum turgidum l. var durum). | tolerance mechanisms to salinity and drought stress are quite complex. plants have developed a complex and elaborate signaling network that ensures their adaptation to this stress. for example, salinity tolerance is thought to be due to three main factors: na(+) exclusion, tolerance to na(+) in the tissues and osmotic tolerance. recently, many transcription factors for tolerance to salt and drought stresses have been identified. in this study, multialignments of conserved domains in dreb1, wrky1 ... | 2012 | 22433076 |
| nitrogen and phosphorus economy of a legume tree-cereal intercropping system under controlled conditions. | considerable amounts of nitrogen (n) and phosphorus (p) fertilizers have been mis-used in agroecosystems, with profound alteration to the biogeochemical cycles of these two major nutrients. to reduce excess fertilizer use, plant-mediated nutrient supply through n(2)-fixation, transfer of fixed n and mobilization of soil p may be important processes for the nutrient economy of low-input tree-based intercropping systems. in this study, we quantified plant performance, p acquisition and belowground ... | 2012 | 22446108 |
| variation in abscisic acid responsiveness of aegilops tauschii and hexaploid wheat synthetics due to the d-genome diversity. | common wheat (triticum aestivum l.) is an allohexaploid that originated from natural hybridization between tetraploid wheat (triticum turgidum) and diploid aegilops tauschii. ae. tauschii is considered one of the potential sources of new genetic variation in abiotic stress tolerance for improving common wheat. abscisic acid (aba) plays an important role in plant adaptation to environmental stresses. in this study, aba responsiveness of 67 ae. tauschii accessions and their synthetic hexaploid whe ... | 2012 | 22531790 |
| novel durum wheat genes up-regulated in response to a combination of heat and drought stress. | we report the effect of heat, drought and combined stress on the expression of a group of genes that are up-regulated under these conditions in durum wheat (triticum turgidum subsp. durum) plants. modulation of gene expression was studied by cdna-aflp performed on rnas extracted from flag leaves. by this approach, we identified several novel durum wheat genes whose expression is modulated under different stress conditions. we focused on a group of hitherto undescribed up-regulated genes in durum ... | 2012 | 22609457 |
| identification and molecular characterisation of hmw glutenin subunit 1by16* in wild emmer. | in this study, a novel y-type high molecular weight glutenin subunit (hmw-gs) in wild emmer wheat triticum turgidum l. var. dicoccoides (körn.) accession ku1952 was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), capillary electrophoresis (ce) and matrix-assisted laser desorption ionisation/time-of-flight/mass spectrometry (maldi-tof-ms). its electrophoretic mobility and molecular weight were similar to those of 1by16 and was designated as 1by16*. the complet ... | 2012 | 22644727 |
| introgression and characterization of a goatgrass gene for a high level of resistance to ug99 stem rust in tetraploid wheat. | the transfer of alien genes to crop plants using chromosome engineering has been attempted infrequently in tetraploid durum wheat (triticum turgidum l. subsp. durum). here, we report a highly efficient approach for the transfer of two genes conferring resistance to stem rust race pgt-ttksk (ug99) from goatgrass (aegilops speltoides) to tetraploid wheat. the durum line das15, carrying the stem rust resistance gene sr47 derived from ae. speltoides, was crossed, and backcrossed, to durum 5d(5b) ane ... | 2012 | 22690376 |
| conservation of socioculturally important local crop biodiversity in the oromia region of ethiopia: a case study. | in this study, we surveyed diversity in a range of local crops in the lume and gimbichu districts of ethiopia, together with the knowledge of local people regarding crop uses, socio-economic importance, conservation, management and existing threats. data were collected using semistructured interviews and participant observation. the study identified 28 farmers' varieties of 12 crop species. among these, wheat (triticum turgidum) and tef (eragrostis tef) have high intra-specific diversity, with 9 ... | 2012 | 22729809 |
| finite element modeling of the cyclic wetting mechanism in the active part of wheat awns. | many plant tissues and organs are capable of moving due to changes in the humidity of the environment, such as the opening of the seed capsule of the ice plant and the opening of the pine cone. these are fascinating examples for the materials engineer, as these tissues are non-living and move solely through the differential swelling of anisotropic tissues and in principle may serve as examples for the bio-inspired design of artificial actuators. in this paper, we model the microstructure of the ... | 2012 | 22791359 |
| genetic map of triticum turgidum based on a hexaploid wheat population without genetic recombination for d genome. | a synthetic doubled-haploid hexaploid wheat population, syndh1, derived from the spontaneous chromosome doubling of triploid f1 hybrid plants obtained from the cross of hybrids triticum turgidum ssp. durum line langdon (ldn) and ssp. turgidum line as313, with aegilops tauschii ssp. tauschii accession as60, was previously constructed. syndh1 is a tetraploidization-hexaploid doubled haploid (dh) population because it contains recombinant a and b chromosomes from two different t. turgidum genotypes ... | 2012 | 22888829 |
| metabolite profiling of a diverse collection of wheat lines using ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry. | genetic differences among major types of wheat are well characterized; however, little is known about how these distinctions affect the small molecule profile of the wheat seed. ethanol/water (65% v/v) extracts of seed from 45 wheat lines representing 3 genetically distinct classes, tetraploid durum (triticum turgidum subspecies durum) (dw) and hexaploid hard and soft bread wheat (t. aestivum subspecies aestivum) (bw) were subjected to ultraperformance liquid chromatography coupled with time-of- ... | 2012 | 22957002 |
| cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (triticum turgidum ssp. durum) and hexaploid (triticum aestivum) wheat grains. | carotenoid β-hydroxylases attach hydroxyl groups to the β-ionone rings (β-rings) of carotenoid substrates, resulting in modified structures and functions of carotenoid molecules. we cloned and characterized two genes (each with three homeologs), hyd1 and hyd2, which encode β-hydroxylases in wheat. the results from bioinformatic and nested degenerate pcr analyses collectively suggest that hyd1 and hyd2 may represent the entire complement of non-heme di-iron β-hydroxylases in wheat. the homeologs ... | 2012 | 23015203 |
| molecular identification of a new powdery mildew resistance gene on chromosome 2bs from triticum turgidum ssp. dicoccum. | powdery mildew caused by the fungal pathogen blumeria graminis f. sp. tritici (bgt), is a destructive foliar disease on wheat in many regions of the world. triticum turgidum ssp. dicoccum (2n=4x=28) shows particular promises as a donor source of useful genetic variation for several traits, including disease resistances that could be introgressed to cultivated wheats. accession mg5323, resistant to powdery mildew, was crossed to the susceptible durum cultivar latino and a set of 122 recombinant i ... | 2012 | 23017904 |
| antioxidant activity of free and bound compounds in quinoa (chenopodium quinoa willd.) seeds in comparison with durum wheat and emmer. | antioxidant activity (aa) of quinoa (chenopodium quinoa willd.) seeds, as well as of durum wheat (triticum turgidum l. ssp. durum desf.) and of emmer (t. turgidum l. ssp. dicoccum schübler) grains, was evaluated by studying hydrophilic (h), lipophilic (l), free-soluble (fsp) and insoluble-bound (ibp) phenolic extracts using the new lipoxygenase/4-nitroso-n,n-dimethylaniline (lox/rno) method, able to simultaneously detect different antioxidant mechanisms, as well as using the oxygen radical absor ... | 2012 | 23057788 |
| coexpression of the high molecular weight glutenin subunit 1ax1 and puroindoline improves dough mixing properties in durum wheat (triticum turgidum l. ssp. durum). | wheat end-use quality mainly derives from two interrelated characteristics: the compositions of gluten proteins and grain hardness. the composition of gluten proteins determines dough rheological properties and thus confers the unique viscoelastic property on dough. one group of gluten proteins, high molecular weight glutenin subunits (hmw-gs), plays an important role in dough functional properties. on the other hand, grain hardness, which influences the milling process of flour, is controlled b ... | 2012 | 23185532 |
| microsatellite mutation rate during allohexaploidization of newly resynthesized wheat. | simple sequence repeats (ssrs, also known as microsatellites) are known to be mutational hotspots in genomes. dna rearrangements have also been reported to accompany allopolyploidization. a study of the effect of allopolyploidization on ssr mutation is therefore important for understanding the origin and evolutionary dynamics of ssrs in allopolyploids. three synthesized double haploid (syndh) populations were made from 241 interspecific f(1 )haploid hybrids between triticum turgidum l. and aegil ... | 2012 | 23202911 |
| identification of novel qtl for resistance to fusarium head blight in a tetraploid wheat population. | most tetraploid durum wheat (triticum turgidum l var. durum) cultivars are susceptible to fusarium head blight (fhb). this study reports novel quantitative trait loci (qtl) associated with fhb resistance. a backcross recombinant inbred line (bcril) population was developed from the cross bgrc3487/2*dt735, and 160 lines were evaluated for resistance to fusarium graminearum schwabe (teleomorph gibberella zeae (schwein. petch) in field trials over 3 years (2008-2010) and to a f. graminearum 3-acety ... | 2012 | 23231604 |
| quantification of microfibril angle in secondary cell walls at subcellular resolution by means of polarized light microscopy. | the cell walls constitute the mechanical support of plants. crystalline cellulose building the walls forms rigid microfibrils that set the stiffness of the cell and the direction in which it expands during growth. therefore, the determination of the directions of the microfibrils is important in both mechanical and developmental assays. we adapted polarized light microscopy to estimate the cellulose microfibril orientations at subcellular resolution. the optical information supplements x-ray sca ... | 2013 | 23240639 |
| prevalence of gene expression additivity in genetically stable wheat allohexaploids. | the reprogramming of gene expression appears as the major trend in synthetic and natural allopolyploids where expression of an important proportion of genes was shown to deviate from that of the parents or the average of the parents. in this study, we analyzed gene expression changes in previously reported, highly stable synthetic wheat allohexaploids that combine the d genome of aegilops tauschii and the ab genome extracted from the natural hexaploid wheat triticum aestivum. a comprehensive gen ... | 2013 | 23278496 |
| characterization of hmw-gs and evaluation of their diversity in morphologically elite synthetic hexaploid wheats. | high molecular weight glutenin subunit composition and variation in 95 elite-1 synthetic hexaploid (sh) wheats (triticum turgidum/aegilops tauschii; 2n = 6× = 42; aabbdd) were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis method (sds-page). twenty two different alleles at glu-1 loci in shs were observed. forty four different patterns of hmw-gs in synthetics were found. this higher hmw glutenin composition was due to higher proportion of d-genome encoded subunits in the ... | 2012 | 23341752 |
| aegilops tauschii single nucleotide polymorphisms shed light on the origins of wheat d-genome genetic diversity and pinpoint the geographic origin of hexaploid wheat. | hexaploid wheat (triticum aestivum, genomes aabbdd) originated by hybridization of tetraploid triticum turgidum (genomes aabb) with aegilops tauschii (genomes dd). genetic relationships between a. tauschii and the wheat d genome are of central importance for the understanding of wheat origin and subsequent evolution. genetic relationships among 477 a. tauschii and wheat accessions were studied with the a. tauschii 10k infinium single nucleotide polymorphism (snp) array. aegilops tauschii consist ... | 2013 | 23374069 |
| synthesis and characterization of advanced durum wheat hybrids and addition lines with thinopyrum chromosomes. | durum wheat (triticum turgidum l., 2n = 4x = 28; aabb genomes) is a natural hybrid-an allotetraploid between 2 wild species, triticum urartu tumanian (aa genome) and aegilops speltoides tausch (bb genome). even at the allotetraploid level, durum wheat can tolerate chromosomal imbalance, for example, addition of alien chromosome 1e of diploid wheatgrass, lophopyrum elongatum. therefore, one way to broaden its genetic base is to add a desirable chromosome(s) from diploid wild relatives. we attempt ... | 2013 | 23396879 |
| identification and mapping of leaf, stem and stripe rust resistance quantitative trait loci and their interactions in durum wheat. | leaf rust (puccinia triticina eriks.), stripe rust (puccinia striiformis f. tritici eriks.) and stem rust (puccinia graminis f. sp. tritici) cause major production losses in durum wheat (triticum turgidum l. var. durum). the objective of this research was to identify and map leaf, stripe and stem rust resistance loci from the french cultivar sachem and canadian cultivar strongfield. a doubled haploid population from sachem/strongfield and parents were phenotyped for seedling reaction to leaf rus ... | 2012 | 23396999 |
| proteome changes in wild and modern wheat leaves upon drought stress by two-dimensional electrophoresis and nanolc-esi-ms/ms. | to elucidate differentially expressed proteins and to further understand post-translational modifications of transcripts, full leaf proteome profiles of two wild emmer (triticum turgidum ssp. dicoccoides tr39477 and ttd22) and one modern durum wheat (triticum turgidum ssp. durum cv. kızıltan) genotypes were compared upon 9-day drought stress using two-dimensional gel electrophoresis and nano-scale liquid chromatographic electrospray ionization tandem mass spectrometry methods. the three genotype ... | 2013 | 23443681 |
| flanking sequence determination and event-specific detection of genetically modified wheat b73-6-1. | in order to establish a specific identification method for genetically modified (gm) wheat, exogenous insert dna and flanking sequence between exogenous fragment and recombinant chromosome of gm wheat b73-6-1 were successfully acquired by means of conventional polymerase chain reaction (pcr) and thermal asymmetric interlaced (tail)-pcr strategies. newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes ... | 2013 | 23449073 |
| evaluation of the safety of ancient strains of wheat in coeliac disease reveals heterogeneous small intestinal t cell responses suggestive of coeliac toxicity. | coeliac disease is a chronic small intestinal immune-mediated enteropathy triggered by dietary gluten in genetically predisposed individuals. since it is unknown if all wheat varieties are equally toxic to coeliac patients seven triticum accessions showing different origin (ancient/modern) and ploidy (di-, tetra- hexaploid) were studied. | 2013 | 23465776 |
| development of a new wheat microarray from a durum wheat totipotent cdna library used for a powdery mildew resistance study. | totipotent cdna libraries representative of all the potentially expressed sequences in a genome would be of great benefit to gene expression studies. here, we report on an innovative method for creating such a library for durum wheat (triticum turgidum l. var. durum) and its application for gene discovery. the use of suitable quantities of 5-azacytidine during the germination phase induced the demethylation of total dna, and the resulting seedlings potentially express all of the genes present in ... | 2013 | 23515937 |
| quantitative imaging of rhizosphere ph and co2 dynamics with planar optodes. | live imaging methods have become extremely important for the exploration of biological processes. in particular, non-invasive measurement techniques are key to unravelling organism-environment interactions in close-to-natural set-ups, e.g. in the highly heterogeneous and difficult-to-probe environment of plant roots: the rhizosphere. ph and co2 concentration are the main drivers of rhizosphere processes. being able to monitor these parameters at high spatio-temporal resolution is of utmost impor ... | 2013 | 23532048 |
| aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. | about 8,000 years ago in the fertile crescent, a spontaneous hybridization of the wild diploid grass aegilops tauschii (2n = 14; dd) with the cultivated tetraploid wheat triticum turgidum (2n = 4x = 28; aabb) resulted in hexaploid wheat (t. aestivum; 2n = 6x = 42; aabbdd). wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker's flour. here we describe sequencing the ae. ta ... | 2013 | 23535592 |
| accumulation of cadmium in near-isogenic lines of durum wheat (triticum turgidum l. var durum): the role of transpiration. | concentrations of cadmium in the grain of durum wheat (triticum turgidum l. var durum) are often above the internationally acceptable limit of 0.2 mg kg(-1). cultivars that vary in concentrations of cadmium in the grain have been identified but the physiology behind differential accumulation has not been determined. three pairs of near-isogenic lines (isolines) of durum wheat that vary in aboveground cadmium accumulation (8982-tl 'high' and 'low', w9260-bc 'high' and 'low', and w9261-bg 'high' a ... | 2011 | 23573025 |
| the genetic study utility of a hexaploid wheat dh population with non-recombinant a- and b-genomes. | to study the d-genome of the wild wheat relative aegilops tauschii cosson at the hexaploid level, we developed a synthetic doubled-haploid (dh) hexaploid wheat population, syndh3. this population was derived from the spontaneous chromosome doubling of triploid f1 hybrid plants obtained from a cross between triticum turgidum ssp. dicoccon pi377655 and a. tauschii ssp. strangulata as66 × ssp. tauschii as87. syndh3 is a diploidization-hexaploid dh population containing recombinant d chromosomes fro ... | 2013 | 23577301 |
| ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley. | this work investigated the importance of the ability of leaf mesophyll cells to control k(+) flux across the plasma membrane as a trait conferring tissue tolerance mechanism in plants grown under saline conditions. four wheat (triticum aestivum and triticum turgidum) and four barley (hordeum vulgare) genotypes contrasting in their salinity tolerance were grown under glasshouse conditions. seven to 10-day-old leaves were excised, and net k(+) and h(+) fluxes were measured from either epidermal or ... | 0 | 23611560 |
| in situ hybridization analysis indicates that 4al-5al-7bs translocation preceded subspecies differentiation of triticum turgidum. | the important cyclic translocation 4al-5al-7bs is an evolutionary signature of polyploidy in wheat. this study aimed to determine its distribution within the subspecies of triticum turgidum l., using genomic in situ hybridization and fluorescence in situ hybridization. as it exists in all eight subspecies, this translocation appeared before the differentiation of the subspecies of t. turgidum. this translocation probably first appeared in t. turgidum subsp. dicoccoides and was then transmitted i ... | 2013 | 23789999 |
| plant uptake and translocation of inorganic and organic forms of selenium. | selenium (se) plays a role in human health: it is an essential trace element but can be toxic if too much is consumed. the aim of this study was to determine which species of se are most rapidly taken up and translocated to above-ground plant tissues. specifically, we wished to determine if organic forms of se in an exposure solution can contribute to the amount of se found in shoot tissue. durum wheat (triticum turgidum) and spring canola (brassica napus) were grown hydroponically, and young se ... | 2013 | 23793939 |
| genetic diversity and population structure of tetraploid wheats (triticum turgidum l.) estimated by ssr, dart and pedigree data. | levels of genetic diversity and population genetic structure of a collection of 230 accessions of seven tetraploid triticum turgidum l. subspecies were investigated using six morphological, nine seed storage protein loci, 26 ssrs and 970 dart markers. the genetic diversity of the morphological traits and seed storage proteins was always lower in the durum wheat compared to the wild and domesticated emmer. using bayesian clustering (k = 2), both of the sets of molecular markers distinguished the ... | 2013 | 23826256 |
| cadmium uptake and partitioning in durum wheat during grain filling. | concentrations of cadmium (cd) in the grain of many durum wheats (triticum turgidum subsp. durum) grown in north american prairie soils often exceed international trade standards. genotypic differences in root-to-shoot translocation of cd are a major determinant of intraspecific variation in the accumulation of cd in grain. we tested the extent to which changes in whole-plant cd accumulation and the distribution of cd between tissues influences cd accumulation in grain by measuring cd accumulati ... | 2013 | 23856013 |
| threshing efficiency as an incentive for rapid domestication of emmer wheat. | the harvesting method of wild and cultivated cereals has long been recognized as an important factor in the emergence of domesticated non-shattering ear genotypes. this study aimed to quantify the effects of spike brittleness and threshability on threshing time and efficiency in emmer wheat, and to evaluate the implications of post-harvest processes on domestication of cereals in the near east. | 0 | 23884398 |
| quality of synthetic hexaploid wheat containing null alleles at glu-a1 and glu-b1 loci. | triticum turgidum ssp. dicoccon pi94668 and pi349045 were identified as containing null alleles at glu-a1 and glu-b1 loci in previous investigation. sequencing of the respective hmw-gs genes ax, bx, ay and by in both accessions indicated equal dna lengths with gene silencing caused by 1 to 4 in-frame stop codon(s) in the open reading frames. six synthetic hexaploid wheat lines were produced by crossing pi94668 or pi349045 with six aegilops tauschii by spontaneous chromosome doubling of unreduced ... | 2013 | 23970079 |
| chay/bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in triticum turgidum ssp. dicoccoides. | high-molecular-weight glutenin subunits (hmw-gss) are of considerable interest, because they play a crucial role in determining dough viscoelastic properties and end-use quality of wheat flour. in this paper, chay/bx, a novel chimeric hmw-gs gene from triticum turgidum ssp. dicoccoides (aabb, 2n=4x=28) accession d129, was isolated and characterized. sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) analysis revealed that the electrophoretic mobility of the glutenin subunit enc ... | 2013 | 24012818 |
| contribution of the ear and the flag leaf to grain filling in durum wheat inferred from the carbon isotope signature: genotypic and growing conditions effects. | the ear, together with the flag leaf, is believed to play a major role as a source of assimilates during grain filling in c3 cereals. however, the intrusive nature of most of the available methodologies prevents reaching conclusive results in this regard. this study compares the carbon isotope composition (δ(13)c) in its natural abundance in the water-soluble fractions of the flag leaf blade and the ear with the δ(13)c of mature kernels to assess the relative contribution of both organs to grain ... | 0 | 24028829 |
| durum wheat and allelopathy: toward wheat breeding for natural weed management. | wheat-derived foodstuffs represent about one-fifth of the calories consumed by humans worldwide. bread wheat (triticum aestivum l.) is one of the most important crops throughout the world, and it has been extensively studied for its allelopathic potential. in contrast, for allelopathy in durum wheat (triticum turgidum ssp. durum), our knowledge is partial and fragmentary. through highlighting recent advances in using allelopathy as a crop-breeding tool, we provide an overview of allelopathy in t ... | 2013 | 24065979 |
| structural analysis of the wheat genes encoding nadh-dependent glutamine-2-oxoglutarate amidotransferases and correlation with grain protein content. | nitrogen uptake and the efficient absorption and metabolism of nitrogen are essential elements in attempts to breed improved cereal cultivars for grain or silage production. one of the enzymes related to nitrogen metabolism is glutamine-2-oxoglutarate amidotransferase (gogat). together with glutamine synthetase (gs), gogat maintains the flow of nitrogen from nh4 (+) into glutamine and glutamate, which are then used for several aminotransferase reactions during amino acid synthesis. | 2013 | 24069228 |
| quantifying relationships between rooting traits and water uptake under drought in mediterranean barley and durum wheat. | in mediterranean regions drought is the major factor limiting spring barley and durum wheat grain yields. this study aimed to compare spring barley and durum wheat root and shoot responses to drought and quantify relationships between root traits and water uptake under terminal drought. one spring barley (hordeum vulgare l. cv. rum) and two durum wheat mediterranean cultivars (triticum turgidum l. var durum cvs hourani and karim) were examined in soil-column experiments under well watered and dr ... | 2014 | 24112696 |
| phosphorylation of a p38-like mapk is involved in sensing cellular redox state and drives atypical tubulin polymer assembly in angiosperms. | reactive oxygen species (ros) imbalance is a stressful condition for plant cells accompanied by dramatic changes in tubulin cytoskeleton. here, evidence is provided that alterations in ros levels directly interfere with the phosphorylation state of a p38-like mapk in the angiosperms triticum turgidum and arabidopsis thaliana. both oxidative stress generators and chemicals inducing ros scavenging or decreasing ros production resulted in the accumulation of a phospho-p46 protein similar to p38-map ... | 2014 | 24138172 |
| relationships among tetraploid wheat (triticum turgidum l.) landrace populations revealed by isozyme markers and agronomic traits. | diversity and relationships among ten tetraploid wheat landrace populations, collected from different localities in the central highlands of ethiopia, were studied using isozyme markers and agronomic traits. this type of analysis in crop species is fundamental for designing optimal germ plasm collection, management practices and for developing an index for parental selection. the populations differed in allelic frequencies. gene-diversity estimates showed that the populations encompass an apprec ... | 1996 | 24162354 |
| engineering of interstitial foreign chromosome segments containing the k(+)/na (+) selectivity gene kna1 by sequential homoeologous recombination in durum wheat. | targeted homoeologous recombination mediated by the absence of the ph1 locus is currently the most efficient technique by which foreign genes can be introgressed into polyploid wheat species. because intra-arm homoeologous double cross-overs are rare, introgressed foreign genes are usually on terminal foreign chromosome segments. since the minimum length of such a segment is determined by the position of a gene in the chromosome, large chromosome segments with undesirable genetic effects are oft ... | 1996 | 24162500 |
| chromosome substitutions of triticum timopheevii in common wheat and some observations on the evolution of polyploid wheat species. | whether the two tetraploid wheat species, the well known triticum turgidum l. (macaroni wheat, aabb genomes) and the obscure t. timopheevii zhuk. (a(t)a(t)gg), have monophyletic or diphyletic origin from the same or different diploid species presents an interesting evolutionary problem. moreover, t. timopheevii and its wild form t. araraticum are an important genetic resource for macaroni and bread-wheat improvement. to study these objectives, the substitution and genetic compensation abilities ... | 1996 | 24162542 |
| the cytogenetics of a triticum turgidum x psathyrostachys juncea hybrid and its backcross derivatives. | psathyrostachys juncea (2n = 2x = 14, nn), a source of barley yellow dwarf (bydv) virus resistance with tolerance to drought and salinity, has been successfully hybridized in its autotetraploid form (2n = 4x = 28, nnnn) as the pollen parent to durum wheat (triticum turgidum l.). the 2n = 4x = 28 (abnn) f1 hybrid has a mean meiotic metaphase-i configuration of 20.29 univalents + 0.29 ring bivalents + 3.36 rod bivalents + 0.14 trivalents. spike length, internode length, glume awn length and lemma ... | 1995 | 24173934 |
| identification and quantification of soluble free, soluble conjugated, and insoluble bound phenolic acids in durum wheat (triticum turgidum l. var. durum) and derived products by rp-hplc on a semimicro separation scale. | a straightforward semimicro separation scale rp-hplc method was developed for the identification and quantification of phenolic acids (pas) occurring as soluble free, soluble conjugated, and insoluble bound compounds, which were independently extracted from wholemeal of durum wheat and from its derived products coarse bran, semolina, and dried pasta. a narrow bore column and a semimicro photodiode array detector (pda) cell, in conjunction with a single quadrupole mass spectrometer, equipped with ... | 2013 | 24175612 |
| durum wheat haploid production using maize wide-crossing. | while anther culture or pollinations with hordeum bulbosum have provided suitable methods for haploid production in bread wheat, they have been largely unsuccessful in durum wheat. pollinations with maize were used in an attempt to produce haploid seedlings and, from these, fertile doubled haploids of durum wheats. moreover, the effect of various concentrations and combinations of a synthetic auxin, 2, 4-dichlorophenoxyacetic acid (2,4-d), kinetin, and an ethylene inhibitor, silver nitrate (agno ... | 1994 | 24177930 |
| use of recombinant substitution lines in the construction of rflp-based genetic maps of chromosomes 6a and 6b of tetraploid wheat (triticum turgidum l.). | rflp-based genetic maps of chromosomes 6a and 6b of triticum turgidum have been constructed using data obtained by the study of triticum turgidum var 'durum' cv 'langdon'-t. t. var 'dicoccoides' recombinant substitution lines (rsls) supplemented with data obtained from f3 families derived from 'langdon' dicoccoides 6a and 6b disomic substitution lines. the average rflp frequencies detected for the two chromosomes in a test of 45 dna clones with six restriction enzymes were 56% and 53%, respectiv ... | 1994 | 24178015 |
| comparative responses of tetraploid wheats pollinated with zea mays l. and hordeum bulbosum l. | ten different tetraploid wheat (triticum turgidum) genotypes were pollinated with maize (zea mays). fertilization was achieved in all ten genotypes and no significant difference in fertilization frequency between the tetraploid wheat genotypes was detected. a mean of 41.1% of pollinated ovaries contained an embryo. all these crosses were characterized by the elimination of the maize chromosomes, and the resulting embryos were haploids. six of the tetraploid wheat genotypes were also pollinated w ... | 1994 | 24190410 |
| enhancement of the salt tolerance of triticum turgidum l. by the kna1 locus transferred from the triticum aestivum l. chromosome 4d by homoeologous recombination. | durum wheat, triticum turgidum l. (2n= 4x=28, genome formula aabb) is inferior to bread wheat, t. aestivum l. (2n=6x=42, genome formula aabbdd), in the ability to exclude na(+) under salt strees, in the ratio of the accumulated k(+) to na(+) in the leaves under salt stress, and in tolerance of salt stress. previous work showed that chromosome 4d has a major effect on na(+) and k(+) accumulation in the leaves of bread wheat. the 4d chromosome was recombined with chromosome 4b in the genetic backg ... | 1994 | 24190475 |
| functional characterization in xenopus oocytes of na+ transport systems from durum wheat reveals diversity among two hkt1;4 transporters. | plant tolerance to salinity constraint involves complex and integrated functions including control of na(+) uptake, translocation, and compartmentalization. several members of the high-affinity k(+) transporter (hkt) family, which comprises plasma-membrane transporters permeable to k(+) and na(+) or to na(+) only, have been shown to play major roles in plant na(+) and k(+) homeostasis. among them, hkt1;4 has been identified as corresponding to a quantitative trait locus (qtl) of salt tolerance i ... | 2014 | 24192995 |
| physical distribution of recombination in b-genome chromosomes of tetraploid wheat. | several studies have indicated a noncorrespondence between genetic and physical distances in wheat chromosomes. to study the physical distribution of recombination, polymorphism for c-banding patterns was used to monitor recombination in 67 segments in 11 b-genome chromosome arms of triticum turgidum. recombination was absent in proximal regions of all chromosome arms; its frequency increased exponentially with distance from the centromere. a significant difference was observed between the distr ... | 1993 | 24193391 |
| characterization of wheat/aegilops ventricosa introgression and addition lines with respect to the m(v) genome. | stable wheat-aegilops introgression lines with 42 chromosomes (h-93), derived by repeated selfing from a cross (triticum turgidum x aegilops ventricosa) x t. aestivum, have been characterized using the following dna probes and isozyme markers: (1) single or low-copy dna fragments from ae. ventricosa; (2) known cdna probes corresponding to α1-thionin, monomeric α-amylase inhibitor, the cm3 subunit of tetrameric α-amylase inhibitor, and sucrose synthase from wheat; (3) anonymous cdna probes from w ... | 1993 | 24193460 |
| storage-protein variation in wild emmer wheat (triticum turgidum ssp.dicoccoides) from jordan and turkey. i. electrophoretic characterization of genotypes. | seed storage-protein variation at theglu-a1,glu-b1 andgli-b1/glu-b3 loci in the tetraploid wild progenitor of wheat,t. dicoccoides, was studied electrophoretically in 315 individuals representing nine populations from jordan and three from turkey. a total of 44 different hmw-glutenin patterns were identified, resulting from the combination of 15 alleles in the a genome and 19 in the b genome. twenty-seven new allelic variants, 12 at theglu-a1 locus and 15 at theglu-b1 locus, were identified by c ... | 1993 | 24193595 |
| storage-protein variation in wild emmer (triticum turgidum ssp.dicoccoides) from jordan and turkey. : ii. patterns of allele distribution. | genetic diversity in the seed storage-proteins encoded at theglu-a1,glu-b1 andgli-b1/glu-b3 loci was studied electrophoretically in 315 individuals belonging to nine populations oft. dicoccoides from jordan and three from turkey. the inter- and intra-population distribution of seed storage-protein alleles at the considered loci and its link with geographical factors were investigated. population differentiation in seed storage-proteins was in some cases very high with very weak correlations with ... | 1993 | 24193601 |
| use of ammi and linear regression models to analyze genotype-environment interaction in durum wheat. | the joint durum wheat (triticum turgidum l var 'durum') breeding program of the international maize and wheat improvement center (cimmyt) and the international center for agricultural research in the dry areas (icarda) for the mediterranean region employs extensive multilocation testing. multilocation testing produces significant genotype-environment (ge) interaction that reduces the accuracy for estimating yield and selecting appropriate germ plasm. the sum of squares (ss) of ge interaction was ... | 1992 | 24202676 |
| intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. | polyploidy or whole-genome duplication is recurrent in plant evolution, yet only a small fraction of whole-genome duplications has led to successful speciation. a major challenge in the establishment of nascent polyploids is sustained karyotype instability, which compromises fitness. the three putative diploid progenitors of bread wheat, with aa, ss (s ∼ b), and dd genomes occurred sympatrically, and their cross-fertilization in different combinations may have resulted in fertile allotetraploids ... | 2013 | 24218593 |
| encoding genes for endosperm proteins in hordeum chilense. | the endosperm proteins encoded by the genome hch in hordeum chilense, tritordeum (amphiploid hordeum chilense x triticum turgidum), common wheat-h. chilense addition lines, and the segregating plants resulting from the cross tritordeum x t. turgidum, were fractionated by three electrophoretical techniques: sds-page, a-page, and bidimensional page. prolamin subunits with a high molecular weight (hmw) were well visualized by sds-page, the a-page technique permitted good resolution for many hordein ... | 1991 | 24221169 |
| high molecular weight glutenin subunit in durum wheat (t. durum). | the diversity of high molecular weight (hmw) glutenin subunits of 502 varieties of durum wheat (triticum durum) from 23 countries was studied using sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). twenty-nine types of patterns were observed with 18 mobility bands. a total of 18 alleles were identified by comparing the mobilities of their subunits to those previously found in hexaploid wheat (t. aestivum) and in triticum turgidum var. dicoccum. five new alleles were detected ... | 1989 | 24227241 |
| biochemical versatility of amphiploids derived from crossing dasypyrum villosum candargy and wheat: genetic control and phenotypical aspects. | the biochemical complexity and its consequence has been investigated in the amphiploids m x v and cs x v derived from crossing the tetraploid wheat triticum turgidum var durum cv 'modoc' and the hexaploid wheat t. aestivum cv 'chinese spring', respectively, with dasypyrum villosum. electrophoretic analysis of variation in six enzyme systems (got, adh, gpi, sod, est, and lpx) and in high molecular weight glutenin seed storage proteins indicated that in the amphiploids these proteins were specifie ... | 1988 | 24232269 |
| biochemical and cytological characterization of wheat/aegilops ventricosa addition and transfer lines carrying chromosome 4m(v.). | the gene encoding a variant of alcohol dehydrogenase, adh-μ, has been found to be associated with the chromosome of the m(v) genome which is present in type 9 wheat/aegilops ventricosa addition line, to which the genes for protein cm-4 and for a phosphatase variant, aph-v, had been previously assigned. transfer line h-93-33, which has 42 chromosomes and has been derived from the cross (triticum turgidum x ae. ventricosa) x t. aestivum, carries genes encoding all three biochemical markers. linkag ... | 1989 | 24232526 |
| high molecular weight glutenin subunit variation in triticum turgidum var. dicoccum. | variation in high molecular weight (hmw) glutenin subunit composition among 167 accessions of dicoccum wheat (triticum turgidum l. var. dicoccum schrank) of diverse origins was investigated using one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page). a total of 20 alleles were identified, and 9 of them were found to be different from those previously detected by payne and lawrence (1983 b) in hexaploid wheat (triticum aestivum l.). the newly discovered alleles enh ... | 1987 | 24240328 |
| subunits of tetrameric α-amylase inhibitors of hordeum chilense are encoded by genes located in chromosomes 4h(ch) and 7h (ch.). | three proteins (components 1, 2, and 4) of the non-prolamin, 70% ethanol soluble fraction from the endosperm of hordeum chilense have been identified as putative subunits of the tetrameric inhibitors active against insect α-amylases. in experiments carried out with the synthetic alloploid tritordeum (h. chilense x triticum turgidum conv. durum), previously described proteins from t. turgidum, designated cm2, cm3 and cm 16, have been also identified as subunits of α-amylase inhibitors. genes for ... | 1987 | 24240344 |
| wheat storage proteins: diversity of hmw glutenin subunits in wild emmer from israel : 1. geographical patterns and ecological predictability. | the diversity of hmw glutenin subunits in the tetraploid wild progenitor of wheat, triticum turgidum var. dicoccoides was studied electrophoretically in 231 individuals representing 11 populations of wild emmer from israel. the results show that (a) the two hmw glutenin loci, glu-a1 and glu-b1, are rich in variation, having 11 and 15 alleles, respectively, (b) genetic variation in hmw glutenin subunits is often severely restricted in individual populations, supporting an island population geneti ... | 1987 | 24240347 |
| chromosomal location of structural genes controlling isozymes in hordeum chilense : 1. 6-phosphogluconate dehydrogenase and malate dehydrogenase. | a study of 6-phosphogluconate dehydrogenase and malate dehydrogenase isozyme expression in triticum turgidum conv. durum /hordeum chilense monosomic addition lines has revealed the location of two structural genes, 6-pgd-h (ch) 2 and mdh-h (ch) 1, on chromosome 1h(ch) of h. chilense. the homoeology between 1h(ch) and other chromosome of triticeae related species is discussed on the basis of isozyme gene analysis. | 1987 | 24241006 |
| chromosomal location of structural genes controlling isozymes in hordeum chilense : 3. esterases, glutamate oxaloacetate transaminase and phosphoglucomutase. | polyacrylamide and starch gel electrophoresis of esterase (est), glutamate oxaloacetate transaminase (got) and phosphoglucomutase (pgm) isozymes in hordeum chilense, triticum turgidum conv. durum, the amphiploid h. chilense x t. turgidum (tritordeum), and the durum wheat/h. chilense monosomic addition lines revealed the chromosomal location of one est locus, two got loci and one pgm locus. loci est-h (ch)1 and got-h (ch)2 were found on chromosome 6h(ch),got-h (ch)3 on chromosome 3h(ch), and pgm- ... | 1987 | 24241192 |
| parental genome expression in synthetic wheats (triticum turgidum sp. × t. tauschii sp.) revealed by two-dimensional electrophoresis of seedling proteins. | two-dimensional gel electrophoresis was conducted on etiolated seedling proteins from two distinct amphiploids (abd1, abd2) and their parental lines (ab1, d1 and ab2, d2), ab1 and ab2 being used as female. on the amphiploid patterns were found all the parental spots except 8 d spots of which 3 are cytoplasmically encoded. one exceptional polypeptide observed in abd1 was present neither in ab1 nor d1. the patterns fromt the amphiploids very closely resemble the co-electrophoresis done with 1/3 d ... | 1987 | 24241568 |
| the inheritance of tetraploid wheat seed peroxidases. | embryo and endosperm peroxidases from dry mature seeds of three subspecies of tetraploid wheat (triticum turgidum l.) were subjected to genetic analysis. the inheritance of eight isozymes (embryo isozymes a2, d1, d2, e and f; and endosperm isozymes b, d and 4) were studied in f2's obtained from different wheat accessions. simple monogenic inheritance producing three banded: one null segregation and two epistatic segregations (9∶7 and 15∶1) were found. in the case of isozymes b, d and 4, monogeni ... | 1985 | 24247340 |
| analysis of the effect of rye chromosomes 4r, 6r and telomeric heterochromatin on 7r on homologous chromosome pairing in pentaploid hybrids (aabbr, aabbd). | meiotic pairing in triticum turgidum cv. ma (4x) with a mean chiasmata frequency of 27.16 per cell was compared with chiasmata frequencies in its hybrids with several triticale strains, 'chinese spring' wheat and its addition lines for 'imperial' rye chromosomes 4r and 6r. in hybrids between 'ma' and x triticosecale cv. 'rosner' the chiasmata frequency was marginally reduced by an average of 1.25%, by 8.8% in hybrids with x triticosecale cv. 'drira' hh and by 6.7% with 'drira' ee (lacking 90% te ... | 1986 | 24248206 |
| a computer-assisted examination of the storage protein genetic variation in 841 accessions of triticum dicoccoides. | triticum turgidum l. var. dicoccoides (wild emmer) is an important genetic resource for increasing the protein content of common wheat (triticum aestivum l.). many studies have shown that the presence or absence of bands in sodium dodecyl sulfate polyacrylamide (sds-page) electrophoregrams of wheat storage proteins to be of a purely genetic character. a total protein extraction and sds-page technique was used to estimate the storage protein genetic variability among 841 accessions of wild emmer ... | 1984 | 24253627 |