Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sequence of a cdna encoding rice (oryza sativa l.) leaf ferredoxin-nadp+ reductase. | 1994 | 8016278 | |
| mrna accumulation and promoter activity of the gene coding for a hydroxyproline-rich glycoprotein in oryza sativa. | the accumulation of the mrna corresponding to the gene coding for a hydroxyproline-rich glycoprotein has been studies in rice. the patterns of gene expression obtained are similar to those observed in maize in regions rich in dividing cells such as the meristematic zones of roots. however, the gene does not seem to be induced by wounding as it is the case in maize. this effect is correlated with the absence of sequences present in the promoter of the maize gene and that have been described as re ... | 1994 | 8018866 |
| poly(a) tail shortening of alpha-amylase mrnas in vegetative tissues of oryza sativa. | the pattern of expression for the entire alpha-amylase gene family in rice vegetative tissues during the developmental growth stages has been studied. we were surprised that the size of rice alpha-amylase mrnas in the young roots, shoots, and different tissues from adult plant was shorter by approximately 250 bases than that in immature or mature embryos and aleurone layers of seed. interestingly, the difference in the size of the alpha-amylase mrnas of rice seed and vegetative tissues is due to ... | 1994 | 8048915 |
| isolation of a monocot 3-hydroxy-3-methylglutaryl coenzyme a reductase gene that is elicitor-inducible. | the rice (oryza sativa) phytoalexins, momilactones and oryzalexins, are synthesized by the isoprenoid pathway. an early step in this pathway, one that is rate-limiting in mammalian systems, is catalyzed by the enzyme 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr). a gene that encodes this enzyme has been isolated from rice, and found to contain an open reading frame of 1527 bases. the encoded protein sequence of the rice hmgr appears to be conserved with respect to other hmgr proteins, a ... | 1994 | 8049366 |
| expressed sequence tags from cultured cells of rice (oryza sativa l.) under stressed conditions: analysis of transcripts of genes engaged in atp-generating pathways. | large-scale sequencing of randomly selected cdna clones was used to isolate numerous genes in rice (oryza sativa l.). total rna used for cdna synthesis was prepared from suspension-cultured cells of rice grown under stressed conditions, such as in saline or nitrogen-starvation conditions. a total of 780 cdna clones were partially sequenced and about 15% could be identified as putative genes. in the library constructed under saline conditions, we identified several genes associated with signal tr ... | 1994 | 8049371 |
| cloning and structural analysis of calmodulin gene from rice. | full-length cdna encoding rice (oryza sativa, cv. nongken 58s) calmodulin (cam) mrna has been cloned using polymerase chain reaction (pcr) with synthesized primers from the sequence of barley cam gene. sequence analysis revealed that the rice gene is 450 bp in length that and its predicted polypeptide consists of 148 amino acids. it differs from those that of barley and alfalfa in only one position, respectively. the nucleotide homology between rice and barley is 90%, between rice and alfalfa is ... | 1993 | 8061233 |
| developmental and light-dependent cues interact to establish steady-state levels of transcripts for photosynthesis-related genes (psba, psbd, psaa and rbcl) in rice (oryza sativa l.). | the steady-state transcript levels for psba, psbd, psaa and rbcl are low in dark-grown rice seedlings as compared to those grown in light. following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. but transcripts for psba and psbd continue to maintain relatively-high levels even after 10 days of growth in light. exposure of 5-day-old dark-grown seedlings to light results in an a ... | 1994 | 8082180 |
| rice tungro bacilliform virus: transcription and translation in protoplasts. | protoplasts from cell suspension cultures of oryza sativa (monocot) and orychophragmus violaceus (dicot) support transcription from the rice tungro bacilliform virus (rtbv) promoter and translation of the resulting mrna despite the presence of a long leader sequence with strong secondary structure and 12 short open reading frames. transcriptional elements located both upstream and downstream of the transcription initiation site are defined by deletion analysis and the functional tata motif is de ... | 1994 | 8091688 |
| expression of a rice homeobox gene causes altered morphology of transgenic plants. | we have isolated a cdna clone encoding a homeobox sequence from rice. dna sequence analysis of this clone, which was designated as oryza sativa homeobox 1 (osh1), and a genomic clone encoding the osh1 sequence have shown that the osh1 gene consists of five exons and encodes a polypeptide of 361 amino acid residues. restriction fragment length polymorphism analysis has shown that osh1 is a single-copy gene located near the phytochrome gene on chromosome 3. introduction of the cloned osh1 gene int ... | 1993 | 8104574 |
| nuclear gene encoding cytosolic triosephosphate isomerase from rice (oryza sativa l.). | 1993 | 8108523 | |
| partial purification and characterization of soluble acid invertases from rice (oryza sativa) leaves. | there are three soluble acid invertases in the leaves of rice. they could be separated by deae1-sephacel chromatography, and named it1, it2 and it3. the molecular weights of it1, it2 and it3, determined by hplc gel filtration chromatography, were 220 kd, 59 kd and 67 kd, respectively. their pi values were 6.2, 5.2 and 4.9, respectively. the ph optima of it1, it2 and it3 were 3.5, 6.0 and 5.5 respectively. all of them could hydrolyze sucrose and raffinose but not maltose, therefore they are all b ... | 1993 | 8136712 |
| root colonization and systemic spreading of azoarcus sp. strain bh72 in grasses. | the invasive properties of azoarcus sp. strain bh72, an endorhizospheric isolate of kallar grass, on gnotobiotically grown seedlings of oryza sativa ir36 and leptochloa fusca (l.) kunth were studied. additionally, azoarcus spp. were localized in roots of field-grown kallar grass. to facilitate localization and to assure identity of bacteria, genetically engineered microorganisms expressing beta-glucuronidase were also used as inocula. beta-glucuronidase staining indicated that the apical region ... | 1994 | 8144457 |
| retrotransposition of a plant sine into the wx locus during evolution of rice. | a new type of plant retroposon, p-sine1, has been found in the wx locus of rice (oryza sativa). it has some structural characteristics similar to those of mammalian sines, such as members of the alu or b1 family. in order to estimate the time at which the integration of p-sine1 into a single locus occurred during rice evolution, we examined the distribution of two members of p-sine1 in several species of the oryza genus by the polymerase chain reaction (pcr). we found that one member of p-sine1 ... | 1994 | 8169958 |
| phylogenetic relationships of 10 grass species: an assessment of phylogenetic utility of the internal transcribed spacer region in nuclear ribosomal dna in monocots. | entire sequences of the internal transcribed spacers (itss) and 5.8s subunit of nuclear ribosomal dna (nrdna) were obtained from nine grass species by direct double-stranded sequencing of polymerase chain reaction (pcr) amplified dna fragments. these sequences from subfamily pooideae (triticum aestivum, crithodium monococcum, sitopsis speltoides, hordeum vulgare, secale montanum, avena longiglumis, bromus inermis, brachypodium distachyon) and subfamily panicoideae (sorghum bicolor) together with ... | 1994 | 8181731 |
| responses to continuous and discontinuous nacl stress of long-term cultured rice (oryza sativa l.) cells. | rice (oryza sativa l.) cells, grown under a continuous stress of 1.5% nacl, produced a homogeneous mass of dry, compact, nodular callus with high regeneration potential, typical of embryogenic cultures. when transferred and subcultured in conditions without salt, the pre-adapted cells underwent several changes, leading to the formation of heterogeneous populations that comprised different cell types. this newly formed callus, in the 6-month period without salt stress, resembled cell populations ... | 1993 | 8184630 |
| sequence and functional analyses of the rice gene homologous to the maize vp1. | the homologous gene of the maize vp1 gene was isolated from rice (oryza sativa). sequence analysis revealed that the rice vp1 gene (osvp1) encodes a protein of 728 amino acids and is interupted by 5 introns at positions identical to those of the maize gene. osvp1 transcript was detected in developing embryo as early as 10 days after flowering and decreased toward maturity. osvp1 transcript was also detected in dry as well as imbided mature embryos. the ability of osvp1 gene product to activate a ... | 1994 | 8193305 |
| characterization of a rice gene coding for a lipid transfer protein. | the cloning and sequence analysis of a gene that encodes a lipid transfer protein (ltp) from rice is reported. a genomic dna library from oryza sativa was screened using a cdna encoding a maize ltp. one genomic clone containing the gene (ltp) was partially sequenced and analyzed. the open reading frame is interrupted by an 89-bp intron. from the results of southern hybridizations, ltp appears to be a member of a small multigenic family. transcripts of the corresponding gene were detected in seve ... | 1994 | 8194762 |
| a rice (oryza sativa l.) cdna encodes a protein sequence homologous to the eukaryotic ribosomal 5s rna-binding protein. | a rice (oryza sativa l.) cdna clone coding for the cytoplasmic ribosomal protein l5, which associates with 5 s rrna for ribosome assembly, was cloned and its nucleotide sequence was determined. the primary structure of rice l5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. these include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three ... | 1993 | 8219074 |
| activity of a maize ubiquitin promoter in transgenic rice. | we have used the maize ubiquitin 1 promoter, first exon and first intron (ubi) for rice (oryza sativa l. cv. taipei 309) transformation experiments and studied its expression in transgenic calli and plants. ubi directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. we exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric g ... | 1993 | 8219091 |
| role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of oryza sativa cv indica. | conditions were optimised for efficient callus induction from seeds of four local indica rice cultivars, gr-3, gr-102, jaya and te-tep. addition of 2,4-d to ms medium at 2.5 mg/l resulted in 100% callus induction. n6 medium was superior to ms medium for callus growth, formation of embryogenic callus as well as regeneration. cultivar te-tep showed highest callus growth while gr-102 the least. addition of casein hydrolysate enhanced growth of callus but did not yield more embryogenic calli. supple ... | 1993 | 8225416 |
| metabolic regulation of alpha-amylase gene expression in transgenic cell cultures of rice (oryza sativa l.). | expression of two genes in the alpha-amylase gene family is controlled by metabolic regulation in rice cultured cells. the levels of ramy3d and ramy3e mrnas in rice cultured cells are inversely related to the concentration of sugar in the culture medium. other genes in the rice alpha-amylase gene family have little or no expression in cultured cells; these expression levels are not controlled by metabolic regulation. a ramy3d promoter/gus gene fusion was metabolically regulated in the transgenic ... | 1993 | 8251627 |
| molecular cloning and nucleotide sequence cdna encoding nucleoside diphosphate kinase of rice (oryza sativa l.). | we isolated a rice cdna encoding nucleoside diphosphate kinase (ndk, ec 2.7.4.6). the deduced amino acid sequence of the rice ndk shows highest homology to spinach ndk-i. the rice ndk gene exhibits a strong codon bias (73.8% gc) in the third position of the codon. dna blot analysis indicated that at least single ndk gene is present in rice genome. | 1993 | 8260630 |
| nascent transcript-binding protein of the pea chloroplast transcriptionally active chromosome. | this study describes the nascent rna-binding protein of the pea chloroplast transcriptional complex. the protein has been identified by photoaffinity labelling of the transcriptionally active chromosome (tac) which utilizes the endogenous plastid dna as template. uv irradiation of lysed chloroplast or the isolated tac under conditions optimized for transcription photocross-links nascent radiolabelled transcripts (up to 250 nucleotides in length) to a 48 kda protein. the photoaffinity labelling o ... | 1993 | 8260634 |
| clusters of interspersed repeated dna sequences in the rice genome (oryza). | we have characterized a repeated dna sequence (rtl122) from rice (oryza sativa l.) with respect to its organization in the rice genome and its distribution among rice and other plants. the results indicate that the rtl122 sequence is interspersed in the rice genome and limited to the genus oryza. it is highly polymorphic and can be used to fingerprint rice varieties. a structure was observed in which several repeated sequences were clustered in dna regions of 15-20 kb. we characterized three bac ... | 1993 | 8270205 |
| characterization of minisatellite sequences from oryza sativa. | two dna sequences were cloned from the genome of cultivated rice (oryza sativa l.) by cross-hybridization with the human minisatellite sequence 33.6. the rice sequences consisted of tandem direct repeats, which showed significant similarity to the 33.6 consensus sequence. profiles capable of distinguishing different rice cultivars were detected by cross-hybridization with a dna probe amplified by the polymerase chain reaction from one of the rice minisatellite sequences. | 1993 | 8270207 |
| cytosolic triosephosphate isomerase is a single gene in rice. | a cdna clone encoding rice (oryza sativa l.) cytosolic triosephosphate isomerase (tpi), an important glycolytic enzyme, was isolated and characterized. the clone (prtpi-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (m(r) 27,060). the identity of this clone was defined by its high homology (85% nucleotide sequence and 89% amino acid sequence identical match) with a maize mrna sequence encoding the cytosolic tpi and with tpis from othe ... | 1993 | 8278510 |
| light-regulated and cell-specific expression of tomato rbcs-gusa and rice rbcs-gusa fusion genes in transgenic rice. | a previously isolated rice (oryza sativa) rbcs gene was further characterized. this analysis revealed specific sequences in the 5' regulatory region of the rice rbcs gene that are conserved in rbcs genes of other monocotyledonous species. in transgenic rice plants, we examined the expression of the beta-glucuronidase (gusa) reporter gene directed by the 2.8-kb promoter region of the rice rbcs gene. to examine differences in the regulation of monocotyledonous and dicotyledonous rbcs promoters, th ... | 1993 | 8278540 |
| nucleotide sequence of a rice root ferredoxin-nadp+ reductase cdna and its induction by nitrate. | a ferredoxin-nadp+ reductase (fnr) cdna was isolated from a lambda gt 11 cdna library constructed from the roots of nitrate-induced rice (oryza sativa l. cv. kinmaze) seedlings. the nucleotide sequence of this clone contains a 1134 nucleotide open reading frame. the n-terminal 62 amino acid stretch was assigned to the transit sequence, followed by 316 residues for the mature protein. the rice root fnr shows only 49% sequence identity to that of the leaf enzyme, but the regions of the binding sit ... | 1994 | 8286405 |
| cloning and sequence analysis of a cdna encoding rice glutaredoxin. | a full-length cdna clone (rasc8) encoding glutaredoxin (thioltransferase) was isolated from a cdna library of an aleurone layer prepared from a developing seed of rice (oryza sativa l.). rasc8, 568bp in length, contained an atg codon and two possible polyadenylation signals, and encoded 112 amino acid residues. cys-pro-phe-cys, which is the active site and a highly conserved sequence among thioltransferases, was found in the deduced amino acid sequence. rasc8 was introduced into an expression ve ... | 1994 | 8287970 |
| cloning and sequencing analysis of a complementary dna for manganese-superoxide dismutase from rice (oryza sativa l.). | 1993 | 8290649 | |
| cdna cloning and sequence analysis of a pathogen-induced thaumatin-like protein from rice (oryza sativa). | 1993 | 8310049 | |
| nucleotide sequence of a rice (oryza sativa) prolamin storage protein gene, rp6. | 1993 | 8310050 | |
| a 127 kda component of a uv-damaged dna-binding complex, which is defective in some xeroderma pigmentosum group e patients, is homologous to a slime mold protein. | a cdna which encodes a approximately 127 kda uv-damaged dna-binding (uv-ddb) protein with high affinity for (6-4)pyrimidine dimers [abramic', m., levine, a.s. & protic', m., j. biol. chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cdna library. the presence of this protein in complexes bound to uv-damaged dna was confirmed by immunoblotting. the human cognate of the uv-ddb gene was localized to chromosome 11. uv-ddb mrna was expressed in all human tissues examined, including c ... | 1993 | 8371985 |
| a transcription map of the chloroplast genome from rice (oryza sativa). | the complete nucleotide sequence (134,525 bp) of the chloroplast genome from rice (oryza sativa l.) contains four rrna genes, 30 trna genes, and over 100 genes that encode proteins. however, expression of only a few of these genes, namely psba, rbcl, and atpb/e, has been detected. we constructed the complete transcription map of rice chloroplast dna by northern hybridization of total rna from rice seedlings, using subclones from a clone bank of rice chloroplast dna as probes. approximately 90% o ... | 1993 | 8381719 |
| anaerobiosis and plant growth hormones induce two genes encoding 1-aminocyclopropane-1-carboxylate synthase in rice (oryza sativa l.). | the plant hormone ethylene is believed to be responsible for the ability of rice to grow in the deepwater regions of southeast asia. ethylene production is induced by hypoxia, which is caused by flooding, because of enhanced activity of 1-aminocyclopropane-1-carboxylic acid (acc) synthase, the key enzyme in the ethylene biosynthetic pathway. we have cloned three divergent members, (os-acs1, os-acs2, and os-acs3), of a multigene family encoding acc synthase in rice. os-acs1 resides on chromosome ... | 1993 | 8389618 |
| agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/beta-glucuronidase gene. | we have successfully transferred and expressed a reporter gene driven by an alpha-amylase promoter in a japonica type of rice (oryza sativa l. cv. tainung 62) using the agrobacterium-mediated gene transfer system. immature rice embryos (10-12 days after anthesis) were infected with an agrobacterium strain carrying a plasmid containing chimeric genes of beta-glucuronidase (uida) and neomycin phosphotransferase (nptii). co-incubation of potato suspension culture (psc) with the agrobacterium inocul ... | 1993 | 8392395 |
| identification of the entire set of transferred chloroplast dna sequences in the mitochondrial genome of rice. | the entire set of transferred chloroplast dna sequences in the mitochondrial genome of rice (oryza sativa cv. nipponbare) was identified using clone banks that cover the chloroplast and mitochondrial genomes. the mitochondrial fragments that were homologous to chloroplast dna were mapped and sequenced. the nucleotide sequences around the termini of integrated chloroplast sequences in the rice mtdna revealed no common sequences or structures that might enhance the transfer of dna. sixteen chlorop ... | 1993 | 8437578 |
| phylogenetic distribution and genetic mapping of a (ggc)n microsatellite from rice (oryza sativa l.). | dna microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. we describe in this article a (ggc)n microsatellite which is widely distributed in eukaryotic genomes. using polymerase chain reaction (pcr) techniques and dna sequencing, we demonstrated for the first time in plant species that a (ggc)n microsatellite locus is moderately polymorphic. six alleles are present at this locus in rice and length polymorphisms are caused by var ... | 1993 | 8448360 |
| use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts. | we have used the bar gene in combination with the herbicide basta to select transformed rice (oryza sativa l. cv. radon) protoplasts for the production of herbicide-resistant rice plants. protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using peg-mediated dna uptake. transformed calli could be selected 2-4 weeks after placing the protoplast-derived calli on medium containing the selective agent, phosphinothricin (ppt), the activ ... | 1993 | 8467080 |
| changes in translatable mrna populations induced in rice seedlings by exposure to freeze-thaw stress. | in order to examine whether changes in gene expression are induced by injury in seedlings of rice (oryza sativa) after a lethal freeze-thaw stress. mrnas were extracted and their in vitro translation products were compared with those of the control plants by polyacrylamide gel electrophoresis. an mrna encoding an entirely new protein with m(r) of ca. 37 kda was detected as early as 30 min of thawing at 28 degrees c. two-dimensional gel electrophoresis revealed the induction of mrnas for at least ... | 1993 | 8485458 |
| enigmatic double-stranded rna in japonica rice. | we have found a linear, 16 kb, double-stranded rna (dsrna) in symptomless japonica rice (oryza sativa l.) that is not found in indica rice (oryza sativa l.). the dsrna was detected in every tissue and at every developmental stage, and its copy number was approximately constant (about 20 copies/cell). double-stranded rna was also detected in two strains of oryza rufipogon (an ancestor of o. sativa). hybridization experiments indicated that the dsrna of o. rufipogon was homologous but not identica ... | 1993 | 8490131 |
| nucleotide sequence of an abundant rice seed globulin: homology with the high molecular weight glutelins of wheat, rye and triticale. | a cdna clone corresponding to a 19 kd salt-soluble globulin of rice (oryza sativa l.) was isolated by screening a lambda gt11 expression library of endosperm mrna with antibodies raised against the purified rice seed alpha-globulin. the cdna contained a single large open reading frame encoding a putative globulin precursor of molecular weight of 21 kd. the polypeptide consists of 182 amino acids and is devoid of lysine residues. computer analysis of the nh2-terminal sequence of the globulin prec ... | 1993 | 8503935 |
| suppression in mitochondrial electron transport is the prime cause behind stress induced proline accumulation. | exposure of six day old rice (oryza sativa) seedlings to salt or cadmium stress lead to an increase in the level of proline with a simultaneous decline in the mitochondrial electron transport activity. mitochondrial electron transport inhibitors - rotenone, antimycin a or potassium cyanide also stimulated proline accumulation in rice seedlings with a concurrent decline in the mitochondrial electron transport activity. four to five fold enhancement in proline level was noted in seedlings after 48 ... | 1993 | 8503937 |
| cloning and characterization of a cdna encoding an 18.0-kda class-i low-molecular-weight heat-shock protein from rice. | a novel cdna clone, oshp18.0 cdna, encoding a rice (oryza sativa l. cv. tainong 67) 18.0-kda heat-shock protein (hsp), was isolated from a cdna library of heat-shocked rice seedlings by use of the rice hsp cdna, oshsp17.3 cdna, as a probe. the sequence showed that oshsp18.0 cdna contains a 749-bp insert encoding an orf of 160 amino acids, with a predicted molecular mass of 18.0 kda and a pi of 7.3. sequence comparison reveals that oshsp18.0 cdna is highly homologous to other low-molecular-weight ... | 1995 | 8522180 |
| polymorphic distribution and molecular diversification of mitochondrial plasmid-like dnas in the genus oryza. | four kinds of circular plasmid-like dna, designated b1, b2, b3 and b4, have been found in the mitochondria of rice (oryza sativa l.). we analyzed the distribution of families of plasmid-like dnas homologous to those of o. sativa in 40 strains of the genus oryza with aa, bb, bbcc, cc, ccdd and ee genomes. plasmid-like dnas were observed only strains having aa, cc and ccdd genomes. the distribution patterns of strains with aa genome were highly polymorphic. we amplified the plasmid-like dnas from ... | 1995 | 8527166 |
| cloning and properties of a rice gene encoding phenylalanine ammonia-lyase. | phenylalanine ammonia-lyase (pal) genomic sequences were isolated from a rice (oryza sativa l.) genomic library using a pcr-amplified rice pal dna fragment as a probe. there is a small family of pal genes in the rice genome. the nucleotide sequence of one pal gene, zb8, was determined. the zb8 gene is 4660 bp in length and consists of two exons and one intron. it encodes a polypeptide of 710 amino acids. the transcription start site was 137 bp upstream from the translation initiation site. rice ... | 1995 | 8534851 |
| analysis of randomly isolated cdnas from developing endosperm of rice (oryza sativa l.): evaluation of expressed sequence tags, and expression levels of mrnas. | using a cdna library prepared from poly(a)+ rna from 10-day-old rice endosperm, partial nucleotide sequences of randomly isolated clones were analyzed. a total of 153 (30.6%) out of 500 cdna clones showed high amino acid identity to previously identified genes. there was significant redundancy in cdnas encoding prolamine and glutelin. about 21.0% of the cdna clones were found to code for seed storage protein genes. consequently, 37 independent genes were identified. using cdna clones encoding gl ... | 1995 | 8541495 |
| a gibberellin-stimulated ubiquitin-conjugating enzyme gene is involved in alpha-amylase gene expression in rice aleurone. | a ubiquitin-conjugating enzyme (ubc) gene, induced by gibberellin (ga) within an hour, was identified in rice (oryza sativa) seeds by the mrna differential display technique. ga inducibility was confirmed by rna hybridization. a full-length ubc cdna clone and a genomic clone have been isolated and sequenced. the deduced amino acid sequence shares a significant identity with several known ubc sequences, which are probably involved in the pathway responsible for degrading short-lived regulatory pr ... | 1995 | 8541504 |
| cloning and nucleotide sequence of a leaf ferredoxin-nitrite reductase cdna of rice. | a ferredoxin-nitrite reductase (ec 1.7.7.1) cdna was isolated and sequenced from a lambda gt 11 cdna library constructed from nitrate-induced greening shoots of rice (oryza sativa l.) seedlings. the nucleotide sequence of the cdna clone contains an open reading frame of 1788 nucleotides. there exists a strong bias for the third codon usage of g/c (95.5%) as in the case of the maize enzyme. the deduced amino acid sequence shows an overall homology to the maize (81%) and the dicot enzymes (70-74%) ... | 1995 | 8541663 |
| open reading frame 176 in the photosynthesis gene cluster of rhodobacter capsulatus encodes idi, a gene for isopentenyl diphosphate isomerase. | isopentenyl diphosphate (ipp) isomerase catalyzes an essential activation step in the isoprenoid biosynthetic pathway. a database search based on probes from the highly conserved regions in three eukaryotic ipp isomerases revealed substantial similarity with orf176 in the photosynthesis gene cluster in rhodobacter capsulatus. the open reading frame was cloned into an escherichia coli expression vector. the encoded 20-kda protein, which was purified in two steps by ion exchange and hydrophobic in ... | 1996 | 8550491 |
| expression of functional oat phytochrome a in transgenic rice. | to investigate the biological functions of phytochromes in monocots, we generated, by electric discharge particle bombardment, transgenic rice (oryza sativa cv gulfmont) that constitutively expresses the oat phytochrome a apoprotein. the introduced 124-kd polypeptide bound chromophore and assembled into a red- and far-red-light-photoreversible chromoprotein with absorbance spectra indistinguishable from those of phytochrome purified from etiolated oats. transgenic lines expressed up to 3 and 4 t ... | 1995 | 8552709 |
| inhibition of gibberellin-induced elongation growth of rice by feruloyl oligosaccharides. | the biological activity of cell wall-derived feruloyl oligosaccharides was investigated using a modified micro-drop bioassay. a feruloyl arabinoxylan trisaccharide (faxx) and a feruloyl arabinoxylan tetrasaccharide (faxxx) were found to inhibit the gibberellin-induced elongation of dwarf rice (oryza sativa l., cv, tan-ginbozu) that had been treated with uniconazole (s-3307), an inhibitor of the biosynthesis of gibberellins. an arabinoxylan trisaccharide (axx) was ineffective. the growth-inhibito ... | 1995 | 8589928 |
| regulation, expression and function of a new basic chitinase gene in rice (oryza sativa l.). | a new basic chitinase gene, designated rc24, was isolated from a rice genomic library. the predicted rc24 protein contains 322 amino acid residues and exhibits 68% to 95% amino acid identity with known class i rice chitinases. rc24 protein expressed in escherichia coli exhibited chitinase activity and strongly inhibited bacterial growth. two transcription start sites of the rc24 gene were mapped by primer extension analysis of both rice native rna and in vitro transcribed rna using a rc24 promot ... | 1996 | 8605293 |
| cloning and characterization of the rice cata catalase gene, a homologue of the maize cat3 gene. | we isolated and sequenced a genomic clone (cata) encoding cat-a catalase, a homologue of the maize catalase isozyme 3 (cat-3) from rice (oryza sativa l.). the 5'-upstream non-coding region had very low similarity with the maize cat3 gene and possible cis elements and sequence motifs in the maize cat3 gene were not evident, except for tata and caat motifs. several sequence motifs found in the promoters of plant seed-specific genes were identified in the 5'-upstream non-coding region of the cata g ... | 1996 | 8605302 |
| rice gibberellin-binding phosphoprotein structurally related to ribulose-1,5-bisphosphate carboxylase/oxygenase activase. | a gibberellin a (ga)-binding protein was identified from rice (oryza sativa l.) leaves by a ligand-binding assay. the dissociation constant of ga-binding protein and ga complex was about 100 nm. this protein has a relative molecular mass of 47 000 and an isoelectric point of 5.1. the partial amino acid sequence of the protein was determined for 54 residues from both the n-terminal and internal regions. a sequence homology search indicated that the amino acid sequence of ga-binding protein was ho ... | 1996 | 8612816 |
| genomic structure of the rice aldolase isozyme c-1 gene and its regulation through a ca 2+ -mediated protein kinase-phosphatase pathway. | complementary and genomic dna clones coding for aldolase c-1, the fourth-type isozyme of aldolase in rice oryza sativa l., have been characterized. the organization of the gene is quite similar to those encoding rice aldolase c-a and a maize cytoplasmic-type aldolase, in that introns are located in the same position. amino acid sequences are highly conserved among cytoplasmic aldolases in plants. expression of the gene in rice callus is activated by a protein phosphatase inhibitor okadaic acid, ... | 1996 | 8616263 |
| molecular cloning of the full-length cdna of (s)-hydroxynitrile lyase from hevea brasiliensis. functional expression in escherichia coli and saccharomyces cerevisiae and identification of an active site residue. | the full-length cdna of (s)-hydroxynitrile lyase (hnl) from leaves of hevea brasiliensis (tropical rubber tree) was cloned by an immunoscreening and sequenced. hnl from h. brasiliensis is involved in the biodegradation of cyanogenic glycosides and also catalyzes the stereospecific synthesis of aliphatic, aromatic, and heterocyclic cyanohydrins, which are important as precursors for pharmaceutical compounds. the open reading frame identified in a 1. 1-kilobase cdna fragment codes for a protein of ... | 1996 | 8621461 |
| centromere mapping and orientation of the molecular linkage map of rice (oryza sativa l.). | rice has become a model cereal plant for molecular genetic research. rice has the most comprehensive molecular linkage maps with more than 2000 dna markers and shows synteny and colinearity with the maps of other cereal crops. until now, however, no information was available about the positions of centromeres and arm locations of markers on the molecular linkage map. secondary and telotrisomics were used to assign restriction fragment length polymorphism markers to specific chromosome arms and t ... | 1996 | 8650237 |
| a methyltransferase for synthesis of the flavanone phytoalexin sakuranetin in rice leaves. | rice (oryza sativa l.) leaves irradiated with short wave uv light accumulated the major rice phytoalexin, flavanone sakuranetin. the extracts from these leaves catalyzed the methylation of the hydroxyl group at position 7 of naringenin to yield sakuranetin, with s-adenosyl-l-methionine as the methyl donor. activity of the naringenin 7-o-methyltransferase was not found in healthy rice leaves but increased upon irradiation with uv light in parallel with increase in sakuranetin. the autoradiogram o ... | 1996 | 8651913 |
| frequency of microsatellite sequences in rice (oryza sativa l.). | this study was undertaken to estimate the relative frequencies of 13 microsatellite motifs in the rice genome as a basis for efficient development of a microsatellite map. two dinucleotide, seven trinucleotide, and four tetranucleotide repeat motifs were end labelled and used as hybridization probes to screen genomic and cdna libraries of rice, cv. ir36. optimal washing temperatures for identification of clones containing specific microsatellite motifs were estimated based on washing temperature ... | 1995 | 8654913 |
| genomic organization of 57 ribosomal protein genes in rice (oryza sativa l.) through rflp mapping. | four hundred cdna clones from rice (oryza sativa l.) callus and root cdna libraries, with a high similarity to about 70 kinds of ribosomal proteins (r-protein) in eukaryotic as well as procaryotic organisms, were identified by their deduced amino acid sequences. southern hybridization of 114 independent cdna clones with total rice genomic dna showed 77 distinct and specific hybridization patterns. of the 77 clones representing the above hybridization patterns, copies of 67 clones corresponding t ... | 1995 | 8654915 |
| molecular cloning and characterization of a cdna for the beta subunit of a g protein from rice. | we isolated a cdna for the beta subunit of a heterotrimeric g protein from rice (oryza sativa l. cv. nipponbare). the amino acid sequence deduced from the cdna was 76% an 94% homologus to the sequences of the beta subunits from arabidopsis and maize (ag beta 1 and zg beta 1), respectively. | 1996 | 8665097 |
| a novel cross-phylum family of proteins comprises a krr1 (ycl059c) gene which is essential for viability of saccharomyces cerevisiae cells. | we demonstrate here that the open reading frame (orf) ycl059c, discovered during the systematic sequencing of chromosome iii [oliver et al., nature 357 (1992) 38-46], codes for a protein essential for yeast: neither spore germination nor cell division occur in strains deleted for this gene. we have cloned the wild-type (wt) gene and shown that it complements the deletion. a relatively abundant rna transcript corresponds to the gene. the protein has no similarity to proteins of known function. in ... | 1996 | 8675026 |
| starch debranching enzyme (r-enzyme or pullulanase) from developing rice endosperm: purification, cdna and chromosomal localization of the gene. | starch debranching enzyme (r-enzyme or pullulanase) was purified to homogeneity from developing endosperm of rice (oryza sativa l. cv. fujihikari) using a variety of high-performance liquid chromatography columns, and characterized. a cdna clone encoding the full length of the rice endosperm debranching enzyme was isolated and its nucleotide sequence was determined. the cdna contains an open reading frame of 2958 bp. the mature debranching enzyme of rice appears to be composed of 912 amino acids ... | 1996 | 8680309 |
| molecular cloning and structural analysis of cdnas that encode 3 small gtp-binding proteins from sugar beet. | isolation of homologous ras-related genes from sugar beet has never been reported. screening cdna library from beta vulgaris l. hilma resulted in the isolation of 3 ras-homologous clones. two of these genes (rab1bv and rab2bv) belong to the rab/ypt group. the deduced polypeptidic sequences from them show strong homologies to ara3 (93% with rab1bv) of arabidopsis thaliana and rgp2 (97% with rab2bv) of oryza sativa. the third gene (rho1bv) belongs to the rho family. the homology of rho1bv protein ... | 1996 | 8680960 |
| the drosophila melanogaster gene vha14 encoding a 14-kda f-subunit of the vacuolar atpase. | a drosophila melanogaster (dm) cdna (vha14) encoding the 14-kda f-subunit of the vacuolar h(+)-atpase (v-atpase) has been cloned via homology with the corresponding manduca sexta (ms) gene. its deduced translation product is a 124-amino-acid polypeptide sharing 90% identity with the ms polypeptide and 50% identity with an analogous polypeptide of saccharomyces cerevisiae, and a more distant similarity to a subunit of the na(+)-transporting atpase of enterococcus hirae. homology was also found wi ... | 1996 | 8682310 |
| isolation and characterization of two cdna clones for mrnas that are abundantly expressed in immature anthers of rice (oryza sativa l.). | the relationship between the length of anthers and the stage of development of microspores was examined in rice (oryza sativa l. cv. hayayuki). anthers of < or = 2 mm and 2.1-2.2 mm in length and those ready to dehiscence were determined to be at the uninucleate, binucleate and trinucleate microspore stage, respectively. two cdnas (yy1 and yy2), representing genes that are specifically expressed in anthers at the uninucleate microspore stage, were isolated and characterized. yy1 cdna encoded an ... | 1996 | 8704128 |
| a computer-based systematic survey reveals the predominance of small inverted-repeat elements in wild-type rice genes. | several recent reports indicate that mobile elements are frequently found in and flanking many wild-type plant genes. to determine the extent of this association, we performed computer-based systematic searches to identify mobile elements in the genes of two "model" plants, oryza sativa (domesticated rice) and arabidopsis thaliana. whereas 32 common sequences belonging to nine putative mobile element families were found in the noncoding regions of rice genes, none were found in arabidopsis genes ... | 1996 | 8710903 |
| characterization of cdna encoding for phosphoglucose isomerase of rice (oryza sativa l.). | two types of genes (pgi-a and pgi-b) encoding phosphoglucose isomerase (pgi; ec 5.3.1.9) were cloned from cdna libraries of rice cultured cells (oryza sativa l.). pgi-a and pgi-b consisted of 2132 and 2030 nucleotides, respectively. the homology between these genes was 93.0% at nucleotide level. the homology scores between these genes in protein coding region and 3' non-coding region were 95.6% and 79.4%, respectively. pgi proteins encoded by pgi-a and pgi-b consisted of 567 and 568 amino acid, ... | 1996 | 8722567 |
| oxidative stress and responses in arabidopsis thaliana and oryza sativa subjected to chilling and salinity stress. | 1996 | 8736786 | |
| a wound- and methyl jasmonate-inducible transcript coding for a myrosinase-associated protein with similarities to an early nodulin. | myrosinase is regarded as a defense-related enzyme in the brassicaceae and is capable of hydrolyzing glucosinolates into various compounds, some of which are toxic. several myrosinase isoenzymes exist, and some of them have been found in association with nonmyrosinase proteins. one of these associated proteins, myrosinase-associated protein (myap), was purified from seeds of brassica napus both in complexes with myrosinase and in a free form. myap is a glycosylated, 40-kd protein with at least o ... | 1996 | 8742330 |
| variations in glutelin and high molecular weight endosperm proteins among subspecies of rice (oryza sativa l.) detected by two-dimensional gel electrophoresis. | variations in endosperm polypeptides among 16 cultivars of rice were analyzed by two-dimensional gel electrophoresis. one glutelin alpha-subunit (alpha 8) with a molecular mass of 32.5 kda was exclusively present in indica cultivars. by contrast, the glutelin alpha 3 subunit, with a molecular mass of 32 kda, was present only in japonica and javanica cultivars. glutelin alpha-subunits alpha 5a and alpha 5b, which have a molecular mass of 32 kda and slightly different isoelectric points, differed ... | 1996 | 8752866 |
| vectors carrying two separate t-dnas for co-transformation of higher plants mediated by agrobacterium tumefaciens and segregation of transformants free from selection markers. | novel 'super-binary' vectors that carried two separate t-dnas were constructed. one t-dna contained a drug-resistance, selection-marker gene and the other contained a gene for beta-glucuronidase (gus). a large number of tobacco (nicotiana tabacum l.) and rice (oryza sativa l.) transformants were produced by agrobacterium tumefaciens lba4404 that carried the vectors. frequency of co-transformation with the two t-dnas was greater than 47% gus-positive, drug-sensitive progeny were obtained from mor ... | 1996 | 8758986 |
| submergence enhances expression of a gene encoding 1-aminocyclopropane-1-carboxylate oxidase in deepwater rice. | partial submergence greatly stimulates internodal growth in deep water rice (oryza sativa l.). previous work has shown that the effect of submergence is, at least in part, mediated by ethylene, which accumulates in the air spaces of submerged internodes. to investigate the expression of the genes encoding ethylene biosynthetic enzymes during accelerated growth of deep water rice, we cloned a 1-aminocyclopropane-1-carboxylate (acc) oxidase cdna (os-aco1) from internodes of submerged plants and me ... | 1996 | 8759917 |
| purification and properties of oryza sativa cu-zn superoxide dismutase. | superoxide dismutase has been purified to homogeneity from oryza sativa germinated seeds growth in the dark. the purified enzyme contained two electrophoretically distinct bands on continuous gel electrophoresis or analytical gel electrofocusing. sds-page showed a single band of an m(r) 15000 while gel chromatography on sephadex g 100 showed a single peak of an m(r) 32000. it contained two cu and two zn ions. the spectra of ultraviolet and visible regions were similar to those of cu-zn mammalian ... | 1996 | 8784923 |
| mitochondrial plasmid-like dnas of the b1 family in the genus oryza: sequence heterogeneity and evolution. | four kinds of circular plasmid-like dna, designated b1, b2, b3 and b4, have been found in the mitochondria of oryza sativa l. with an aa genome. three novel b1-homologous mitochondrial plasmid-like dnas, designated, m1, m2 and m3, were isolated in the present study from strains with cc and ccdd genomes in the genus oryza. we cloned and sequenced these dnas and found that the sequences of these molecules have wide regions of homology. b1, m2 and m3 each lack about 300 bp of a region that is prese ... | 1995 | 8790446 |
| isolation of mammalian pol beta type dna polymerase in shoot tips of germinated seedlings of ir-8 rice (oryza sativa l.). | a single polypeptide of approx. 67 kda mol.wt. with dna polymerase activity has been chromatographically purified from shoot tips of 72 hr. grown germinated seedlings of rice (oryza sativa.l.cv ir-8). an approx. 4800 fold enrichment of specific activity was measured by the incorporation of 3h-dtmp into trichloroacetic acid insoluble fraction, using activated calf thymus dna as template-primer. the enzyme uses different types of dna but not rna as a template. the enzyme requires mg+2, high kcl, a ... | 1996 | 8799334 |
| epidermal growth factor binding proteins in rice (oryza sativa l.) leaves. | a ligand blotting experiment using [125i]epidermal growth factor (egf) showed that there are egf binding proteins in rice leaves. egf binding proteins have isoelectric points and relative molecular masses of 5.5/35 kda, 7.5/50 kda and 8.3/40 kda, respectively. the amino acid sequences of the n-terminal and internal regions of the egf binding proteins were determined. the amino acid sequences of 35 kda protein and 40 kda were found to be homologous with those of the photosystem ii oxygen-evolving ... | 1996 | 8799499 |
| purification and properties of a basic endo-1,3-beta-glucanase from rice (oryza sativa l.). | a 1,3-beta-glucanase purified from rice grain is a 33 kda monomer with a pi of > or = 10.4. the enzyme was determined to be an endo-1,3-beta-glucanase (ec 3.2.1.39) by end product analysis using laminaria digitata laminarin as substrate. its amino-terminal amino acid sequence revealed strong homology to an endo-1,3-beta-glucanase from barley. | 1996 | 8819316 |
| cool-temperature-induced chlorosis in rice plants. | we have established an experimental system for mimicking the phenomenon of cool-temperature-induced chlorosis (ctic) in rice plants (oryza sativa l.). rice seedlings were initially grown in darkness under cool-temperature conditions and then exposed to light and warm conditions to follow the expression of ctic. induction of ctic in the sensitive cultivar (cv surjamukhi) was bimodally dependent on the temperatures experienced during the initial growth in darkness. ctic was maximally induced betwe ... | 1996 | 8819872 |
| phytohormone-regulated beta-amylase gene expression in rice. | the expression of beta-amylase genes in rice (oryza sativa) and its regulation by phytohormones gibberellic acid (ga) and abscisic acid (aba) were examined. upon germination beta-amylase is synthesized de novo in aleurone cells and (ga) is not required. exogenous addition of ga does not enhance the beta-amylase activity, while aba inhibits the beta-amylase activity, mrna accumulation, and the germination of rice seeds. ga can reverse aba inhibition of beta-amylase expression, but not aba inhibit ... | 1996 | 8843940 |
| isolation and characterization of cdnas encoding vacuolar h(+)-pyrophosphatase isoforms from rice (oryza sativa l.). | the vacuolar h(+)-pyrophosphatase (v-ppase) is an electrogenic h+ pump, which was found in the plant vacuolar membrane. two cdna clones (ovp1 and ovp2) encoding the v-ppase were isolated from cultured rice (oryza sativa l.) cells and subsequently sequenced. the sequence analysis has revealed that ovp1 contains 2316 nucleotides of open reading frame (orf) and 362 nucleotides of the 3'-untranslated region, whereas ovp2 comprises 2304 nucleotides of orf and 312 nucleotides of the 3'-untranslated re ... | 1996 | 8843945 |
| evidence for genomic changes in transgenic rice (oryza sativa l.) recovered from protoplasts. | the occurrence of genomic modifications in transgenic rice plants recovered from protoplasts and their transmission to the self-pollination progeny has been verified with the random amplified polymorphic dna (rapd) approach. the plant was the indica-type rice (oryza sativa l.) cultivar chinsurah boro ii. the analysed material was: (1) microspore-derived embryogenic rice cells grown in suspension culture, (2) transgenic plants recovered from protoplasts produced from the cultured cells and (3) th ... | 1996 | 8866891 |
| the rab16b promoter of rice contains two distinct abscisic acid-responsive elements. | to localize abscisic acid (aba)-inducible gene expression of rab16 genes, rab16a promoter was linked to the gusa reporter gene encoding beta-glucuronidase and introduced into rice (oryza sativa l.) plants. the activity of rab16a promoter was induced by aba and osmotic stresses in various tissues of vegetative and floral organs. in anthers and embryos, rab16a promoter was active in the absence of aba. to elucidate cis-elements of the rab16 promoter that confer aba-inducible expression, variously ... | 1996 | 8883374 |
| phosphorylation of a protein (pp56) is related to the regeneration of rice cultured suspension cells. | short-term cultured suspension cells of rice (oryza sativa l.) are capable of regeneration, but not in long-term culture. for clarification of the mechanism of regeneration, protein phosphorylation in short-term and long-term cultured suspension cells was compared by two dimensional-polyacrylamide gel electrophoresis. a 56 kda protein having an isoelectric point of 4.5 was phosphorylated in vitro in short-term cultured suspension cells, but was not phosphorylated after regeneration. this protein ... | 1996 | 8888618 |
| higher plants contain homologs of the bacterial cela genes encoding the catalytic subunit of cellulose synthase. | in spite of much effort, no one has succeeded in isolating and characterizing the enzyme(s) responsible for synthesis of cellulose, the major cell wall polymer of plants. we have characterized two cotton (gossypium hirsutum) cdna clones and identified one rice (oryza sativa) cdna that are homologs of the bacterial cela genes that encode the catalytic subunit of cellulose synthase. three regions in the deduced amino acid sequences of the plant cela gene products are conserved with respect to the ... | 1996 | 8901635 |
| dna fingerprints of rice (oryza sativa) obtained from hypervariable chloroplast simple sequence repeats. | the aim of this research was to develop a convenient polymerase chain reaction-based assay that would allow intraspecific chloroplast variability to be detected. our approach is based on the detection of length polymorphism within chloroplast mononucleotide microsatellite loci. information from the fully sequenced rice chloroplast genome was used to identify 12 regions with a minimum of ten uninterrupted mononucleotide repeats. primers flanking these repeats were used in conjunction with polymer ... | 1996 | 8914327 |
| development of microsatellite markers and characterization of simple sequence length polymorphism (sslp) in rice (oryza sativa l.). | microsatellite markers containing simple sequence repeats (ssr) are a valuable tool for genetic analysis. our objective is to augment the existing rflp map of rice with simple sequence length polymorphisms (sslp). in this study, we describe 20 new microsatellite markers that have been assigned to positions along the rice chromosomes, characterized for their allelic diversity in cultivated and wild rice, and tested for amplification in distantly related species. our results indicate that the geno ... | 1996 | 8914521 |
| physicochemical and serological characterization of rice alpha-amylase isoforms and identification of their corresponding genes. | we have identified, purified, and characterized 10 alpha-amylase isoforms from suspension-cultured rice (oryza sativa l.) cells having different isoelectric point values. they had distinguishable optimum temperatures for enzymatic activity and molecular sizes. the results of immunoblotting indicated that polyclonal anti-a + b antibodies bound well to isoforms a, b, y, and z but weakly or not at all to e, f, g, h, i, and j. however, the anti-a + b antibodies inhibited the enzyme activities of onl ... | 1996 | 8934629 |
| characterization of three rice basic/leucine zipper factors, including two inhibitors of embp-1 dna binding activity. | the promoter of the wheat em gene contains elements with a cacgtg core sequence (g-boxes), which are recognized by embp-1, a wheat basic/leucine zipper (bzip) protein. g-boxes are required for em expression in response to the phytohormone abscisic acid and for transactivation by the viviparous-1 protein (vp1) using transient expression systems. in order to identify other factors that are part of the transcriptional complex that associates with g-boxes, we have screened a rice (oryza sativa) cdna ... | 1996 | 8940135 |
| two-dimensional electrophoresis of plant proteins and standardization of gel patterns. | proteins of two plants, arabidopsis thaliana and rice (oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i) comigration of external standard marker proteins with resultant horizontal and vertical stripes in the gel, and (ii) deblocking with a vapor of aqueous heptafluorobutyric acid for n-acetylserine. approximately 5000 protein spots were separated from both the five tissues of arabidopsis and the nine tissues of rice. over one hundred spots were ... | 1996 | 8964270 |
| phylogenetic relationships in a-genome species of rice as revealed by rapd analysis. | in order to clarify the phylogenetic relationships in a-genome species of rice, rapd analysis was carried out using 29 accessions (8 oryza sativa, 6 o. glaberrima, 13 o. perennis, and 2 o. breviligulata). their total dna was used as template for pcr with 14 decamer primers. the amplified products were electrophoresed in agarose gel, and the banding patterns were observed. average number of total amplified bands for each accession using 14 primers was 84.6. by comparing the banding patterns, the ... | 1996 | 8979372 |
| mechanism of safening action of dymuron and its two monomethyl analogues against bensulfuron-methyl injury to rice (oryza sativa) | the comparative efficacy and mechanism of action of the herbicide dymuron and its two optically active monomethyl analogues, (r)-1-(alpha-methylbenzyl)-3-(p-tolylurea) (r-mbtu) and s-mbtu, as safeners of rice against injury from bensulfuron-methyl were investigated. bioassays using etiolated seedlings of rice (oryza sativa l., cv. lemont), grown either in agar or in liquid media containing bensulfuron-methyl, showed that this sulfonylurea herbicide is a potent inhibitor of rice root growth (i50 ... | 1996 | 8980030 |
| rna expression patterns of a type 2 metallothionein-like gene from rice. | a type 2 metallothionein-like gene from rice, osmt-2 (oryza sativa metallothionein-like gene-2), was isolated in its cdna form and sequenced. by northern analyses osmt-2 expression was shown to be induced under stress by sucrose starvation, heat shock and, to a lesser extent, abscisic acid, but not excess metals, including copper. its response to sucrose starvation was transient and different from osmt-1, a type 1 metallothionein-like gene of rice inducible by copper. these results suggest that ... | 1996 | 8980501 |
| chalcone synthase in rice (oryza sativa l.): detection of the chs protein in seedlings and molecular mapping of the chs locus. | the chalcone synthase is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. the chs gene and its protein product in rice has been investigated. the presence of a chalcone synthase (chs) protein in rice seedlings and its developmental stage-specific expression has been demonstrated by western analysis. the chalcone synthase of rice was found to be immunologically similar to that of maize. a rice cdna clone, os-chs cdna, encoding chalcone synthase, ... | 1996 | 8980525 |
| novel dna probes capable of discriminating indica and japonica rice cultivars. | we obtained two dna clones, g93 (1.6kb) and g318 (1.7kb) from indica rice (oryza sativa l.) cultivar culture 340. southern hybridization analysis indicated that both dna probes hybridized with dna of indica cultivars but not with japonica cultivars. some javanica cultivars dnas did not hybridize with these probes. nucleotide sequence (400bp) of a part of g93 showed a 65.8% g + c content. these novel dna probes can be used for the study of evolutional fingerprinting of rice cultivars. | 1996 | 8988367 |
| [the goal-oriented project "pathology of environmental pollutants" (m.u.r.s.t. 40%)]. | the purpose of this study was to estimate to toxicity bound to effluent water, pore water and bed sediment sampled twice during 1994 (spring and autumn) from 16 tributaries of po river. toxicity was determined by using a battery of bioassays (artemia salina, dugesiagonocephala and thamnocephalus platyurus) and a battery of phytoassays, including oryza sativa and triticum sativum. results show that practically no toxicity is found in effluent water, mild adverse effects are obtained with sediment ... | 1995 | 8991825 |
| t-dna integration into genomic dna of rice following agrobacterium inoculation of isolated shoot apices. | this paper establishes that the isolated shoot meristem of monocotyledons can be infected and transformed using agrobacterium. since this explant from nearly any cereal cultivar can rapidly regenerate into a plant, using this explant effectively eliminates the genotype regeneration restrictions to cereal crop transformation allowing direct transformation of elite germplasm. shoot apices of oryza sativa l. tropical japonica, cv. maybelle were explants used for cocultivation, and gene transfer was ... | 1996 | 9002612 |
| phytoalexin production by amino acid conjugates of jasmonic acid through induction of naringenin-7-o-methyltransferase, a key enzyme on phytoalexin biosynthesis in rice (oryza sativa l.). | amino acid conjugates of jasmonic acid are found to elicit production of the flavonoid phytoalexin, sakuranetin in rice leaves. the elicitation is shown to arise from induction of naringenin 7-o-methyltransferase, a key enzyme of sakuranetin biosynthesis. the (-)-phenylalanine conjugate, one of the active compounds, is characterized by high activity for both sakuranetin and enzyme induction and low phytotoxicity against rice growth. both (+)-enantiomers of the conjugates and free amino acids do ... | 1997 | 9013895 |
| expression characteristics of os-acs1 and os-acs2, two members of the 1-aminocyclopropane-1-carboxylate synthase gene family in rice (oryza sativa l. cv. habiganj aman ii) during partial submergence. | deepwater rice can grow in the regions of southeast asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. one such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flood water levels. ethylene is considered to be the trigger of this growth response because deepwater conditions not only trap ethylene in submerged organs, but also enhance the activ ... | 1997 | 9037160 |