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efficiency of some in vitro allergy tests for evaluating atopy in children and adults.in recent years, an increase in the prevalence of allergic diseases has been reported, in industrialised countries. it is also a problem in non-industrialised countries, but its extent is less well documented. in this study, inhalant screening allergens (alatop), food screening allergens (fp5e), and total and specific ige were measured in 237 allergic patients (164 adults and 73 children) in order to examine the distribution of common allergens, the percentage of atopy/non-atopy among allergic c ...199910616753
quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus.using perennial ryegrass infected with an acremonium transformant carrying the escherichia coli beta-d-glucuronidase gene (gusa) (gus system) under control of a constitutive promoter, we have developed methods for the quantitative extraction of endophyte-associated gus activity from plant material. fluorometric assays of these extracts allow quantitative assessment of the distribution of endophyte-associated gus activity within single plants (tillers) with high resolution. fluorescence microscop ...199710627142
substrate preference profiles of proteases released by allergenic pollens.pollens are important triggers for allergic asthma and seasonal rhinitis. we have recently reported that proteases released by major allergenic pollens can injure airway epithelial cells in vitro. disruption of epithelial integrity by proteases released following deposition of pollens on mucosal surfaces could promote sensitization and induce inflammation.200010718856
use of length heterogeneity pcr and fatty acid methyl ester profiles to characterize microbial communities in soil.in length heterogeneity pcr (lh-pcr) a fluorescently labeled primer is used to determine the relative amounts of amplified sequences originating from different microorganisms. labeled fragments are separated by gel electrophoresis and detected by laser-induced fluorescence with an automated gene sequencer. we used lh-pcr to evaluate the composition of the soil microbial community. four soils, which differed in terms of soil type and/or crop management practice, were studied. previous data for mi ...200010742258
ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (medicago sativa), white clover (trifolium repens), red clover (trifolium pratense) or perennial ryegrass (lolium perenne).two experiments were performed with post-valve t-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (cp) and amino acid (aa) digestibilities. the control diets (c1 and c2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (medicago sativa) or white clover (trifolium repens) meal in expt 1 and ...199910743486
histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation.histamine is a physiological mediator which exerts both effector and regulatory functions through its receptors on various cells. the aim of the study was to investigate changes in histamine receptor expression on peripheral blood lymphocytes affected by stimulation with both specific and nonspecific stimuli. lymphocytes were obtained from both healthy and allergic subjects. cells were incubated with various allergens (mixed grass pollen, lolium perenne, dermatophagoides pteronyssinus 1, bee ven ...200010807051
synthetic zeolites as amendments for sewage sludge-based compost.the effects of incorporating a synthetic zeolite (zeolite p) in a range of concentrations (0.1-1.0 w:w) into an experimental horticultural compost, derived from sewage sludge, have been investigated. the impact of zeolite treatment on time-related changes of the labile zinc, copper, iron and manganese pools within the compost was compared to lime incorporation (5% w:w) and to a proprietary unamended peat-based compost. addition of 0.5% and 1.0% zeolite significantly reduced labile zinc over a 90 ...200010819210
duration of regrowth of ryegrass (lolium perenne) effects on grazing behavior, intake, rumen fill, and fermentation of lactating dairy cows.the relative importance of duration of sward regrowth and fill and fermentation in the rumen on the control of grazing time and intake rate during the first grazing session of the day was studied. four lactating dairy cows were allowed to graze ryegrass (lolium perenne) swards, with five different regrowth periods after mowing (6, 9, 16, 22, and 30 d). the cows were allowed to graze until they stopped voluntarily (cessation of grazing activity for at least 15 min). before and after grazing the r ...200010821574
lawn parameters influencing abundance and distribution of the hairy chinch bug (hemiptera: lygaeidae).management of lawns that promotes conditions detrimental to the development of insect pests may represent a valuable environmentally benign turfgrass management strategy. in the cool-humid region of quebec, canada, we investigated 45 lawns infested with hairy chinch bug, blissus leucopterus hirtus montandon, to identify lawn parameters related to its distribution and abundance. kentucky bluegrass, creeping bentgrass, and perennial ryegrass, respectively, accounted for 55.8, 19.6, and 9.3% of the ...200010826187
expulsion of allergen-containing materials from hydrated rye grass (lolium perenne) pollen revealed by using immunogold field emission scanning and transmission electron microscopy.several studies demonstrated episodes of grass pollen-induced allergic asthma after heavy rainfalls. it has been hypothesized that these asthma attacks might be due to the release of respirable allergen-bearing particles from pollen cytoplasm.200010856148
effect of breed and age on the voluntary intake and the micromineral status of non-pregnant sheep. 1. estimation of voluntary intake.twenty-four sheep were used to test breed and age differences in voluntary intake (vi). the sheep were divided into four groups of six animals each: suffolk mature ewes (sm), suffolk yearlings (sy), rambouillet mature ewes (rm), and rambouillet yearlings (ry). the animals grazed alternatively two mixed pastures containing trifolium repens latum, lolium perenne and pennisetum clandestinum from 07:00 to 16:30 hours and remained penned overnight. voluntary intake was estimated using chromic sesquio ...200010867320
effect of breed and age on the voluntary intake and the micromineral status of non-pregnant sheep. ii. micromineral status.twenty-four non-pregnant sheep, divided into four groups of six animals each, were used to test breed (suffolk and rambouillet) and age (mature (six years old) and yearling (16 months old) ewes) differences in micromineral status. the animals grazed alternatively two mixed pastures containing trifolium repens latum, lolium perenne and pennisetum clandestinum from 07:00 to 16:30hours, and remained penned overnight. hand-plucked forage samples, blood and wool samples were collected on days 1 (exce ...200010867321
amino acids as a nitrogen source in temperate upland grasslands: the use of dual labelled ((13)c, (15)n) glycine to test for direct uptake by dominant grasses.it is becoming increasingly apparent that soil amino acids are a principal source of nitrogen (n) for certain plants, and especially those of n-limited environments. this study of temperate upland grasslands used glycine-2-(13)c-(15)n and ((15)nh4)(2)so(4) labelling techniques to test the hypothesis that plant species which dominate 'unimproved' semi-natural grasslands (festuca-agrostis-galium) are able to utilise amino acid n for growth, whereas those plants which dominate 'improved' grasslands ...200010920354
effects of zinc and influence of acremonium lolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (lolium perenne l. cv apollo).the effects of zinc on growth, mineral content, chlorophyll a fluorescence, and detoxifying enzyme activity (ascorbate peroxidase (apx), ec 1.11.1.11; superoxide dismutase (sod), ec 1.15.1.1) of ryegrass infected or not by acremonium lolii, and treated with nutrient solution containing 0-50 mm znso(4) were studied. the introduction of zinc induces stress with a decrease in growth at 1, 5 and 10 mm znso(4) and a cessation of growth at 50 mm znso(4), in ryegrass plants infected by a. lolii or not. ...200010948221
response of a soil bacterial community to grassland succession as monitored by 16s rrna levels of the predominant ribotypes.the composition of predominant soil bacteria during grassland succession was investigated in the dutch drentse a area. five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. the activity of the predominant bacteria was monitored by direc ...200010966420
portal net appearance of amino acids in growing pigs fed a barley-based diet with inclusion of three different forage meals.the net absorption of amino acids (aa) in young pigs fed a barley-based control diet (c) and diets where barley was replaced by 200 g/kg fresh weight of dried lucerne (medicago sativa; l20), white clover (trifolium repens; w20) or perennial ryegrass (lolium perenne; pr20) meal was studied. castrated male pigs were fitted with permanent catheters in the hepatic portal vein and mesenteric artery, and the hepatic portal net absorption of aa was estimated from the porto-arterial plasma concentration ...200011103219
cloning and expression of a distinct subclass of plant thioredoxins.mrnas encoding a novel thioredoxin were isolated from pollen rna of lolium perenne (lptrx), hordeum bulbosum (hbtrx), phalaris coerulescens (ptrx) and secale cereale (sctrx). the cdnas contain a single orf of 393 bp encoding a protein of 131 amino acids. the predicted proteins showed highest homology to plant thioredoxins of the h class yet form a distinct subgroup that is characterized by a high level of sequence conservation (95.4-97.7% identity). genbank searches revealed additional members o ...200011106422
overseeding endophytic perennial ryegrass into stands of kentucky bluegrass to manage bluegrass billbug (coleoptera: curculionidae).studies were conducted to determine the utility of overseeding endophyte enhanced varieties of perennial ryegrass, lolium perenne l., into existing stands of kentucky bluegrass, poa pratensis l., to manage bluegrass billbug, sphenophorus parvulus gyllenhal, larval populations and their damage. two commercial varieties of endophyte enhanced perennial ryegrass (repell ii and triple play) were overseeded at two different rates in the field. s. parvulus larval populations, their damage, and turfgras ...200011142296
the effect of applying sodium fertilizer on the rate of digestion of perennial ryegrass and white clover incubated in rumen liquor, with implications for ruminal tympany in cattle.a high herbage k:na ratio increases the risk of ruminal tympany in cattle, which may relate to digestion rate. experiment 1 examined whether in vitro digestibility of ryegrass was affected by nacl fertilizer or by na concentration in artificial saliva. fertilizer na increased grass digestibility, but na in artificial saliva decreased it, probably due to the energy cost of sodium exclusion from bacteria. increased herbage digestibility with fertilizer na is therefore not due to additional na, but ...200111145830
predicting the transfer of radiocaesium from organic soils to plants using soil characteristics.a model predicting plant uptake of radiocaesium based on soil characteristics is described. three soil parameters required to determine radiocaesium bioavailability in soils are estimated in the model: the labile caesium distribution coefficient (kd1), k+ concentration in the soil solution [mk] and the soil solution-->plant radiocaesium concentration factor (cf, bq kg-1 plant/bq dm-3). these were determined as functions of soil clay content, exchangeable k+ status, ph, nh4+ concentration and org ...200111202684
a terminal flower1-like gene from perennial ryegrass involved in floral transition and axillary meristem identity.control of flowering and the regulation of plant architecture have been thoroughly investigated in a number of well-studied dicot plants such as arabidopsis, antirrhinum, and tobacco. however, in many important monocot seed crops, molecular information on plant reproduction is still limited. to investigate the regulation of meristem identity and the control of floral transition in perennial ryegrass (lolium perenne) we isolated a ryegrass terminal flower1-like gene, lptfl1, and characterized it ...200111244130
anaerobically digested poultry slaughterhouse wastes as fertiliser in agriculture.chemical and physical analysis, 27-d plant growth assays with carrot (daucus carota) and chinese cabbage (brassica campestris var. chinensis), and 5-d phytotoxicity assays with chinese cabbage and perennial ryegrass (lolium perenne) were used to investigate the suitability of anaerobically digested poultry slaughterhouse waste for fertiliser in agriculture and the effect of aerobic post-treatment on the properties of the digested material. the digested material appeared to be rich in nitrogen. i ...200111265792
a comparison of manometric and membrane-electrode procedures for measuring rates of leaf decay.two instruments were compared for consistency of decay-rate measurements of four leaf types: lolium perenne, typha domingenesis, casuarina cunninghamiana and eucalyptus polyanthemos, via progressive biological oxygen demand (bod). the instruments were (a) a dissolved oxygen probe (i.e. an oxygen-selective membrane electrode) used in two different modes: (1) measurements of sacrificial samples that were successively discarded, and (2) repeated measurements of the same sample, and (b) a mercury fr ...200111349381
association between feeding perennial ryegrass (lolium perenne cultivar grasslands impact) containing high concentrations of ergovaline, and health and productivity in a herd of lactating dairy cows.perennial ryegrasses are frequently infected with fungal endophyte (neotyphodium lolii) to increase the resistance of the plant to insect damage. unfortunately, a side effect of endophyte infection can be the production of alkaloids, including lolitrem b and ergovaline, that produce toxic effects in animals. a significant 4.6 litre decrease in milk production in a herd of holstein-friesian dairy cows was associated with high concentrations of ergovaline in ryegrass silage. simultaneously, milk s ...200111349413
correlation of endophyte toxins (ergovaline and lolitrem b) with clinical disease: fescue foot and perennial ryegrass staggers.fescue foot, summer syndrome, reproductive problems, and ryegrass staggers are all diseases of livestock related to endophyte toxins in pasture grasses. range finding experiments and case studies of fescue foot relative to ergovaline toxin found in endophyte infected tall fescue and lolitrem b present in endophyte infected perennial ryegrass were conducted. within 42 d of initiating a feeding trial with chopped tall fescue straw containing 825 ppb ergovaline and at environmental temperatures of ...200111383653
release of allergen-bearing cytoplasm from hydrated pollen: a mechanism common to a variety of grass (poaceae) species revealed by electron microscopy.the release of submicronic particles from grass pollen after rainfall was suggested to be responsible for outbreaks of grass pollen asthma. recently, we provided evidence for the release of respirable allergen-bearing particles from hydrated ryegrass (lolium perenne ) pollen as a possible explanation for this phenomenon.200111447390
adaptation of ruminococcus flavefaciens resulting in increased degradation of ryegrass cell walls.this study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. no significant increase in degradation was detected for lucerne, barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium ruminococcus flavefaciens strain 17 on each of these substrates. significantly increased substrate degradation by r. flavefaciens strain 17 was however observed after 23 subcultures on perennial ryegrass. the ...200111451517
allergen-induced basophil activation: cd63 cell expression detected by flow cytometry in patients allergic to dermatophagoides pteronyssinus and lolium perenne.in this study, we determined by flow cytometry the percentage of basophils activated after in vitro stimulation by allergens and expressing the cd63 marker. the diagnostic reliability of the technique was assessed as well as its correlation with other in vitro diagnostic parameters.200111467990
surface runoff pollution by cattle slurry and inorganic fertilizer spreading: chemical oxygen demand, ortho-phosphates, and electrical conductivity levels for different buffer strip lengths.as a way of dealing with the removal of pollutants from farming practices generated wastewater in the eu, we investigate the effect of spreading cattle slurry and inorganic fertiliser on 8 x 5 m2 and 8 x 3 m2 areas, referred to surface runoff chemical oxygen demand (cod), ortho-phosphates (o-p) and electrical conductivity (ec) levels, and the efficiency of grass buffer strips of various lengths in removing pollutants from runoff. the experimental plot was a 15% sloped lolium perenne pasture. sur ...200111496670
elimination of ergovaline from a grass-neotyphodium endophyte symbiosis by genetic modification of the endophyte.the fungal endophytes neotyphodium lolii and neotyphodium sp. lp1 from perennial ryegrass (lolium perenne), and related endophytes in other grasses, produce the ergopeptine toxin ergovaline, among other alkaloids, while also increasing plant fitness and resistance to biotic and abiotic stress. in the related fungus, claviceps purpurea, the biosynthesis of ergopeptines requires the activities of two peptide synthetases, lps1 and lps2. a peptide synthetase gene hypothesized to be important for erg ...200111592979
detection of endophyte toxins in the imported perennial ryegrass straw.from 1997 to 1999, 29 cases of disorders were detected in cattle and horses that had been fed ryegrass straw imported from the u.s.a. these animals showed symptoms resembling ryegrass staggers and the clinical signs disappeared after removal of the straw. endophytic hyphae were detected in the seeds of all straw samples that were responsible for the clinical cases. lolitrem b concentrations in the straw ranged between 972 and 3740 ppb. ergovaline concentrations were between 355 and 1300 ppb. eve ...200111642270
black cutworm (lepidoptera: noctuidae) larval emigration and biomass in mixtures of endophytic perennial ryegrass and kentucky bluegrass.studies examined the possibility that mixtures of endophytic perennial ryegrass and kentucky bluegrass provide resistance against larvae of the black cutworm, agrotis ipsilon (hufnagel). we hypothesized that resistance against a. ipsilon in such stands would stem from the influence of kentucky bluegrass on a. ipsilon growth and behavior rather than the influence of endophytic perennial rvegrass. in replicated greenhouse experiments, black cutworm larvae initially emigrated more quickly from pots ...200111681682
in planta regulation of extension of an endophytic fungus and maintenance of high metabolic rates in its mycelium in the absence of apical extension.the fungus neotyphodium lolii is an endophytic symbiont. it grows in the intercellular spaces of the perennial ryegrass lolium perenne, producing secondary metabolites which enhance the fitness of the association over that of uninfected l. perenne. we report that the average number of hyphal strands in a given section of a leaf remains constant during the life of a leaf, indicating synchrony of leaf and hyphal extension, including cessation of hyphal extension when leaf extension ceases. we used ...200111722882
ryegrass host genetic control of concentrations of endophyte-derived alkaloids.endophytic fungi in pasture grasses produce alkaloids which affect invertebrate and vertebrate herbivores. while the competence to produce an alkaloid is a property of the fungus, the host plant may moderate fungal activity. host genetic influence on endophyte activity was studied in perennial ryegrass (lolium perenne l.) infected with a common strain of neotyphodium lolii (latch, christensen & samuels) glenn, bacon & hanlin. progeny seedling families of a partial diallel cross and their 12 pare ...200211756253
accumulation of hmx (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine) in indigenous and agricultural plants grown in hmx-contaminated anti-tank firing-range soil.to investigate their potential for phytoremediation, selected agricultural and indigenous terrestrial plants were examined fortheir capacity to accumulate and degrade the explosive octahydro-1 ,3,5,7-tetra nitro-1,3,5,7-tetrazocine (hmx). plant tissue and soil extracts were analyzed for the presence of hmx and possible degradative metabolites using high-performance liquid chromatography with diode-array uv detection (hplc-uv), micellar electrokinetic chromatography with diode-array uv detection ...200211811476
dynamic and steady-state responses of inorganic nitrogen pools and nh(3) exchange in leaves of lolium perenne and bromus erectus to changes in root nitrogen supply.short- and long-term responses of inorganic n pools and plant-atmosphere nh(3) exchange to changes in external n supply were investigated in 11-week-old plants of two grass species, lolium perenne and bromus erectus, characteristic of n-rich and n-poor grassland ecosystems, respectively. a switch of root n source from no(-)(3)to nh(4)(+) caused within 3 h a 3- to 6-fold increase in leaf apoplastic nh(4)(+) concentration and a simultaneous decrease in apoplastic ph of about 0.4 ph units in both s ...200211842177
[the effects of the primary growth of two ryegrasses on the dynamics of changes in digestibility and feed intake by sheep].dynamics of changes in digestibility and feed intake by sheep of two ryegrass species during primary growth environmental impacts can cause short-term variations in chemical composition and feeding value of grass swards during growth and maturation and induce concomitant changes of intake of these grasses by animals. continuous digestion trials are effective in observing the dynamics of these variations. therefore this study was conducted to evaluate the effects of maturity-related alterations i ...200111906563
influence of flavor on goat feeding preferences.goat feeding preferences for straw pellets flavored with ryegrass (lolium perenne, cv. belida) or white clover (trifolium repens, cv. huia) aromatic extracts, obtained by means of a cold aromatic extraction method, were assessed with cafeteria trials. prior to the trials, odor differences between the two plant species, the two aromatic extracts, and the straw pellets sprayed with the two aromatic extracts were verified using sensory analysis performed by 30 human panelists. since odor difference ...200211925067
grass species and endophyte effects on survival and development of fall armyworm (lepidoptera: noctuidae).grass selections including 10 zoysiagrasses, 18 paspalums, 34 bermuda grasses, tall fescue, creeping red fescue, and perennial ryegrasses with and without endophyte were evaluated for potential resistance to fall armyworm, spodoptera frugiperda (j. e. smith), larvae. laboratory evaluations assessed the degree of antibiosis among >70 grass lines to first-instar fall armvworms. when all parameters measured were considered, the trend in resistance to fall armyworm among endophyte-infected (e+) and ...200212020031
degradation of fresh ryegrass by methanogenic co-cultures of ruminal fungi grown in the presence or absence of fibrobacter succinogenes.the ability of five ruminal fungi in syntrophic co-culture with the methanogen methanobrevibacter smithii to degrade perennial ryegrass ( lolium perenne) stem fragments and leaf blades was studied to determine the susceptibilities of non-autoclaved fresh tissues to fungal degradation. autoclaving did not significantly increase fungal degradation of stem fragments but strongly increased degradation of leaf blades by a species of caecomyces. in methanogenic co-cultures, non-autoclaved stem fragmen ...200212029527
does decreased mowing frequency enhance alkaloid production in endophytic tall fescue and perennial ryegrass?tall fescue, festuca arundinacea, and perennial ryegrass, lolium perenne. are widely infected with fungal endophytes (neotyphodium spp.). the symbiosis between plant and fungus leads to synthesis of alkaloids that have been shown to be either toxic or act as feeding deterrents against insect pests. as cultural practices have the potential to regulate production of plant secondary metabolites, we evaluated the influence of mowing frequency on the levels of major alkaloids in tall fescue and peren ...200212049232
phytoremediation of subarctic soil contaminated with diesel fuel.the effects of several plant species, native to northern latitudes, and different soil amendments, on diesel fuel removal from soil were studied. plant treatments included scots pine (pinus sylvestris), poplar (populus deltoides x wettsteinii), a grass mixture (red fescue, fesuca rubra; smooth meadowgrass, poa pratensis and perennial ryegrass, lolium perenne) and a legume mixture (white clover, trifolium repens and pea, pisum sativum). soil amendments included npk fertiliser, a compost extract a ...200212118697
attenuation of groundwater contamination caused by cattle slurry: a plot-scale experimental study.infiltration of contaminants was investigated in a flat pasture plot lolium perenne l. which received 250 m3/ha of cattle slurry. lysimeters and piezometers had previously been installed in the plot to sample groundwater at different depths. water samples were analysed for ph, conductivity, nh4(+), no3-, orthophosphate, cl-, na+, k+, ca2+, mg2+ and chemical oxygen demand (cod), and for faecal coliforms and faecal streptococci. contaminant concentrations in water samples taken in lysimeters at a ...200212139326
clinical characteristics of patients with mustard hypersensitivity.although mustard is frequently consumed in spain and elsewhere, only isolated case reports of mustard allergy have been reported. no large series of case studies have been published.200212197573
is lolium pollen from an urban environment more allergenic than rural pollen?allergy to grass pollen is a highly prevalent allergic disease. hay fever is more predominant in urban than in rural areas, despite the increasingly smaller areas of surrounding grassland. the effect of vehicle exhaust pollutants, mainly diesel particles, and other industrial sources of atmospheric pollution leading to plant damage has been implicated in this phenomenon.200212199966
a novel fungal protease expressed in endophytic infection of poa species.the fungus acremonium typhinum produces a novel endoprotease during symbiotic endophytic infection of the grass, poa ampla. this protease is unusual because it is highly active in the presence of sodium dodecyl sulfate. the enzyme is a thiol-containing serine protease and is localized to a crude membrane fraction. similar protease activity has been detected in endophyte-infected poa autumnalis and poa sylvestris plants. expression of this protease may be important in endophytic infection of poa ...199312231854
effects of grazing undrenched weaner deer on chicory or perennial ryegrass/white clover pasture on the viability of gastrointestinal nematodes and lungworms.this study determined the in vitro effects on the viability of internal parasites of grazing undrenched weaner deer on either chicory (cichorium intybus) or perennial ryegrass (lolium perenne)/white clover (trifolium repens) pasture. one experiment investigated the hatching and development of gastrointestinal nematode eggs and larvae, and the development and motility of l1 lungworm (dictyocaulus eckerti) larvae, and a second experiment used larval migration inhibition assays to test the viabilit ...200212371691
[allergens and risk factors in pediatric patients with allergic seasonal conjunctivitis].the seasonal allergic conjunctivitis is an inflammatory disease of the ocular surface that affects mainly children, with predominance of male sex. it is an immunological disease with a typical reaction of hypersensitivity type 1 (ige), resulted from several biological reactions (antigen-antibody) and it's associated to several risk factors.200212374042
alpha-tocopherol concentrations and case life of lamb muscle as influenced by concentrate or pasture finishing.two experiments were conducted to evaluate alpha-tocopherol accumulation in muscle of lambs finished on pasture or concentrates. the objective for exp. 1 was to compare accumulation of alpha-tocopherol in the longissimus muscle of pasture-fed lambs to that of lambs fed three concentrations (15, 150, and 300 iu/kg of dm) of supplemental vitamin e (all rac alpha-tocopheryl acetate) in all-concentrate diets. the objective in exp. 2 was to investigate the effect of duration of supplemental vitamin e ...200212413072
lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep.condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. however, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. the aim of this study was to investigate the specific effects of condensed tannins from lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (lolium perenne ...200212489781
morphological variation in different host populations of abacarus hystrix (acari: prostigmata: eriophyoidea).the present systematic classification of eriophyoidea is widely recognised as artificial and not reflecting plant-herbivore linkage. quantitative description of host-related morphological variation can provide the basic information needed to improve the eriophyoid taxonomic system and enhance our understanding of mechanisms generating this variation. the purpose of this study was to investigate quantitative morphological traits of populations of cereal rust mite abacarus hystrix (nalepa 1896) li ...200212537292
feed intake of young double-muscled bulls fed on grass and supplemented with sugar-beet pulp.daily dry matter intake in young growing double-muscled bulls, fed indoors on grass, was estimated based on forty-four intake data from 28 animals, ageing at least five months and weighing up to 400 kg live weight. intake was measured during five consecutive days using one of eighteen cuts of grass. fresh meadow grass (mainly lolium perenne) was fed ad libitum and two kg dried sugar-beet pulp was offered per animal and per day. animal live weight averaged 278 +/- 82 kg and mean total daily dry m ...200212556046
[responses of agricultural crops of free-air co2 enrichment].over the past decade, free-air co2 enrichment (face) experiments have been conducted on several agricultural crops: wheat(triticum aestivum l.), perennial ryegrass (lolium perenne), and rice(oryza sativa l.) which are c3 grasses; sorghum (sorghum bicolor (l.) möench), a c4 grass; white clover (trifolium repens), a c3 legume; potato (solanum tuberosum l.), a c3 forb with tuber storage; and cotton (gossypium hirsutum l.) and grape (vitis vinifera l.) which are c3 woody perennials. using reports fr ...200212557686
the physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (lolium perenne).we have characterized a cold-induced, boiling stable antifreeze protein. this highly active ice recrystallization inhibition protein shows a much lower thermal hysteresis effect and displays binding behavior that is uncharacteristic of any afp from fish or insects. ice-binding studies show it binds to the (1 0 1 0) plane of ice and ftir studies reveal that it has an unusual type of highly beta-sheeted secondary structure. ice-binding studies of both glycosylated and nonglycosylated expressed for ...200312573283
the effect of birdsfoot trefoil (lotus corniculatus) and chicory (cichorium intybus) on parasite intensities and performance of lambs naturally infected with helminth parasites.conventionally, farmers rely upon the routine use of anthelmintics to control helminth parasites and their use has proved highly cost-effective. however, several factors, including the emergence of helminths resistant to pharmaceutical anthelmintics, are forcing farmers to seek alternative approaches to parasite control. studies in new zealand have shown that some alternative forages may reduce parasitic infestation in sheep. in the current study, it was found that under uk environmental conditi ...200312581592
the development of trichostrongylus colubriformis larvae on a range of herbage species or on plots of differing topographical aspect.five "contaminations", where faeces containing trichostrongylus colubriformis eggs were deposited on pasture and serially recovered, were used to compare the rate of decline of faecal mass and larval development. in the first three contaminations, faeces from a common source were deposited on swards of browntop (agrostis capillaris cv grasslands muster), ryegrass (lolium perenne cv grassland nui), white clover (trifolium pratense cv grassland tahora), or onto bare ground in the late spring, summ ...200312591198
surveys of microfungi in a former industrial area in duisburg-nord.one hundred and forty microfungi (ascomycetes and deuteromycetes) were collected in the "landschaftspark duisburg-nord" located in north rhine-westphalia. new hosts for rarely found fungi are recorded for the first time. new for germany are massaria inquinans (tode) de not. and nitschkia grevillei (rhem) nannf. on acer pseudoplatanus l., pirottaea nigrostriata graddon on artemisia vulgaris l., ceratopycnis clematidis höhn. on clematis vitalba l., dasyscyphus aff. humuli (w. phillips) dennis on h ...200212701427
impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of central europe.the impact of land use intensity on the diversity of arbuscular mycorrhizal fungi (amf) was investigated at eight sites in the "three-country corner" of france, germany, and switzerland. three sites were low-input, species-rich grasslands. two sites represented low- to moderate-input farming with a 7-year crop rotation, and three sites represented high-input continuous maize monocropping. representative soil samples were taken, and the amf spores present were morphologically identified and count ...200312732553
carbon mineralisation and plant growth in soil amended with compost samples at different degrees of maturity.the carbon and nitrogen mineralisation of a composting mixture of brewing yeast and lemon tree prunings was studied, at different degrees of stabilisation of this matrix, within an incubation experiment in soil. meanwhile, a growth test in pots with ryegrass (lolium perenne l.) was carried out using the selected soil and equal amounts of the composting mixture taken at different maturation steps, in order to evaluate the additions of these organic amendments in terms of fertilising value. sample ...200312739731
epidemiology of atopy in 220 children. diagnostic reliability of skin prick tests and total and specific ige levels.we have prospectively studied 220 children attending our division because they suffered from atopic dermatitis (ad), asthma, and allergic rhinitis (ar), to assess the epidemiology of atopic diseases, and effectiveness of the diagnostic tests commonly used in allergic children.200312754457
diversity of arbuscular mycorrhizal fungi colonising roots of the grass species agrostis capillaris and lolium perenne in a field experiment.analysis of arbuscular mycorrhizal (am) fungal diversity through morphological characters of spores and intraradicular hyphae has suggested previously that preferential associations occur between plants and am fungi. a field experiment was established to investigate whether am fungal diversity is affected by different host plants in upland grasslands. indigenous vegetation from plots in an unimproved pasture was replaced with monocultures of either agrostis capillaris or lolium perenne. modifica ...200412768382
[legume cross-reactivity].legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ...200312783766
effects of earthworms and organic litter distribution on plant performance and aphid reproduction.human management practices and large detritivores such as earthworms incorporate plant litter into the soil, thereby forming a heterogeneous soil environment from which plant roots extract nutrients. in a greenhouse experiment we investigated effects of earthworms and spatial distribution of (15)n-labelled grass litter on plants of different functional groups [ lolium perenne (grass), plantago lanceolata (forb), trifolium repens (legume)]. earthworms enhanced shoot and root growth in l. perenne ...200312844255
morphological compatibility of white clover and perennial ryegrass cultivars grown under two nitrate levels in flowing solution culture.the effects of nitrate (no3-) supply on shoot morphology, vertical distribution of shoot and root biomass and total nitrogen (n) acquisition by two perennial ryegrass (lolium perenne l.) cultivars (aberelan and preference) and two white clover (trifolium repens l.) cultivars (grasslands huia and aberherald) were studied in flowing nutrient culture. cultivars were grown from seed as monocultures and the clovers inoculated with rhizobium. the 6-week measurement period began on day 34 (grasses) and ...200312876188
neozygites abacaridis sp. nov. (entomophthorales), a new pathogen of phytophagous mites (acari, eriophyidae).a new entomopathogenic fungus, described here as neozygites abacaridis n. sp. (zygomycetes: entomophthorales), has been found on the mites abacarus hystrix, aculodes dubius, and a. mckenziei (acari: eriophyidae). it differs from other neozygites species affecting mites by its small, globose primary conidia, short-ovoid, smoky coloured capilliconidia, and very short capillary conidiophores-which are usually not longer than the spore length. this pathogen infected mite individuals in autumn (from ...200312877829
whole-body fluxes and partitioning of amino acids to the mammary gland of cows fed fresh pasture at two levels of intake during early lactation.the utilisation of essential amino acids (eaa) by the mammary gland of lactating dairy cows fed fresh forages was studied to provide basic information useful in designing strategies to increase the production of milk protein from pasture-fed dairy cows. the relationship between the flux of eaa in the whole body and their uptake by the mammary gland was determined in four cows in early lactation (length of time in milk 44 (sd 14.5) d) producing 21 (sd 4.0) kg milk/d. the cows were maintained in m ...200312908887
does mowing height influence alkaloid production in endophytic tall fescue and perennial ryegrass?the mutualistic symbiosis following infection of tall fescue, festuca arundinacea, and perennial ryegrass, lolium perenne, by fungal endophyte (neotyphodium spp.) results in the production of alkaloids that are feeding deterrents or toxic to insects and livestock. if the levels of the alkaloids can be manipulated by cultural practices in the grasses that are used for home lawns and golf courses, this could alleviate the need for pesticide applications in urban environments. we evaluated the infl ...200312918918
fate of urine nitrogen in three soils throughout a grazing season.the fate of 15n-labeled cattle (bos taurus) urine (52 g n m(-2)), applied to a 0.4-m2 surface area on three dates between may and october to three different pasture soils, was studied using 2-m2 lysimeters. over a period of two years, the sward recovered most of the 15n, but the amount recovered decreased with application date (62% in spring to 17% in fall). however, n uptake by ryegrass (lolium perenne l.) in year 2 showed that some nitrogen came from the previous year's urine application. the ...200312931896
hot alkali-labile linkages in the walls of the forage grass phalaris aquatica and lolium perenne and their relation to in vitro wall digestibility.the factors affecting in vitro dry matter digestibility (ivdmd) of fully mature internodes of 150 lines of the forage grass, phalaris aquatica, and internodes of 100 lines of perennial ryegrass (lolium perenne), harvested just after anthesis, were investigated. the relationships between ivdmd and the contents of acetyl bromide lignin, and ester-ether linkages between lignin and wall polysaccharides, measured by hydroxycinnamic acids (hcas) released by 4 m naoh at 170 degrees c respectively, were ...200312943783
stimulation of soil nitrification and denitrification by grazing in grasslands: do changes in plant species composition matter?stimulation of nitrification and denitrification by long term (from years to decades) grazing has commonly been reported in different grassland ecosystems. however, grazing generally induces important changes in plant species composition, and whether changes in nitrification and denitrification are primarily due to changes in vegetation composition has never been tested. we compared soil nitrification- and denitrification-enzyme activities (nea and dea, respectively) between semi-natural grassla ...200312955489
isolation and characterisation of an invertase cdna from perennial ryegrass (lolium perenne).an invertase (lpft2) cdna from perennial ryegrass was isolated and sequenced. nucleotide sequence analysis revealed an orf of 2016 bp encoding a protein of 671 amino acids. lpft2 is 76% identical to sugarcane soluble acid invertase, and contains invertase and fructosyltransferase functional domains. lpft2 is present as a single copy gene and maps to the distal region of lg6 in perennial ryegrass. the expression pattern analysis of lpft2 revealed transcript accumulation in seedlings and in mature ...200312964866
characteristics of pollinosis caused by betula in patients from ourense (galicia, spain).a retrospective study was performed to describe the features of the pollinosis caused by betula in the area of ourense, spain. the pollen count was carried out with a lanzoni volumetric hirts spore trap (1993-2000). the betula pollen represented 5% over the annual total (annual mean quantity: 965 grains). it was present in the air from march to mid-may. the highest peaks took place in april (maximum values mean: 131 grains/m3). the medical records of 222 patients (mean age 25.66 years) diagnosed ...200312968397
comparison of growth performance of lolium perenne l., dactylis glomerata l. and agropyron elongatum (host.) p. beauv. for erosion control in turkey.this study was carried out in plastic containers to compare growth performances of perennial ryegrass, orchardgrass and tall wheatgrass to be given priority in revegetation studies in turkey. three pre-germinated seeds of each grass species were planted separately into the soil in the black plastic containers. seedlings were harvested 2, 4 and 6 months after planting pre-germinated seeds and measured for percentage of seedling emergence, rooting depth, height growth, leaf and tiller development ...200312974411
biochemical outcome of blocking the ergot alkaloid pathway of a grass endophyte.neotyphodium sp. lp1, an endophytic fungus from perennial ryegrass (lolium perenne), produces the mycotoxin ergovaline in infected grasses, whereas a mutant in which a particular peptide synthetase gene is knocked out does not. we examined the impact of this knockout on other constituents of the ergot alkaloid pathway. two simple lysergic acid amides, ergine and a previously undescribed amide, were eliminated by the knockout. lysergic acid accumulated in the knockout endophyte, but quantities we ...200314558758
the infection process of colletotrichum graminicola and relative aggressiveness on four turfgrass species.detached 3-week-old leaves of agrostis palustris, lolium perenne, poa annua, and poa pratensis were inoculated with conidial suspensions of two isolates of colletotrichum graminicola obtained from a. palustris. inoculated leaves were incubated at 23 degrees c under high relative humidity (>95%). the infection process was investigated by light microscopy from 2 to 168 h after inoculation (ai). spore germination was observed within 2 h ai, appressoria within 6 h ai, and penetration pores within 8 ...200314569284
mass spectrometric analysis of electrophoretically separated allergens and proteases in grass pollen diffusates.pollens are important triggers for allergic asthma and seasonal rhinitis, and proteases released by major allergenic pollens can injure airway epithelial cells in vitro. disruption of mucosal epithelial integrity by proteases released by inhaled pollens could promote allergic sensitisation.200314577842
beneficial properties of single-domain antibody fragments for application in immunoaffinity purification and immuno-perfusion chromatography.we explored the possibility to apply single-domain antibodies from camelidae for immunoaffinity purification of the ice structuring protein (isp) from lolium perenne, which modifies ice crystal growth and therefore has potential application in medicine, biotechnology, agriculture and (frozen) foods. using phage display together with an appropriate selection method, a group of candidate fragments was isolated from a llama-derived immune library. affinity chromatography using a purposely selected ...200314642809
discrimination against 13c during degradation of simple and complex substrates by two white rot fungi.changes in isotopic 13c signatures of co2-c evolved during decomposition of a sugar (glucose), a fatty acid (palmitic acid), a protein (albumin), a structural biopolymer (lignin) and bulk plant tissue (aerial shoots from lolium perenne) were monitored over a period of 76 days. all materials were sterilized and inoculated with either of two different species of white rot fungi, phanerochaete chrysosporium or coriolus versicolor, and incubated in sealed bottles at 28 degrees c. the co2 concentrati ...200314648898
the effect of dietary forage on the development and survival of helminth parasites in ovine faeces.studies in new zealand and the uk have shown that lambs grazing birdsfoot trefoil (lotus corniculatus) or chicory (cichorium intybus) have reduced parasite intensities compared to lambs grazing ryegrass swards. however, data in the literature on the influence of forages on helminth parasites is equivocal and the underlying mechanisms by which different forage diets may affect these parasites have not been fully determined. the aim of the experiments reported here was to investigate the hypothesi ...200314651879
the determinant step in ergot alkaloid biosynthesis by an endophyte of perennial ryegrass.many cool-season grasses harbor fungal endophytes in the genus neotyphodium, which enhance host fitness, but some also produce metabolites--such as ergovaline--believed to cause livestock toxicoses. in claviceps species the first step in ergot alkaloid biosynthesis is thought to be dimethylallyltryptophan (dmat) synthase, encoded by dmaw, previously cloned from claviceps fusiformis. here we report the cloning and characterization of dmaw from neotyphodium sp. isolate lp1, an endophyte of perenni ...200414732265
temperature control of the development of frost hardiness in two populations of leptospermum scoparium.seedlings of leptospermum scoparium j.r. et g. forst (manuka) originating from seed from a low altitude coastal site (auckland) and from a high altitude inland site (desert road) were grown for 96 days in four controlled environments to compare the relationship between growth temperature and frost hardening. day/night temperature treatments were 12/6, 12/3, 12/0 and 12/-3 degrees c. frost hardiness was determined at 14-day intervals by exposing whole seedlings to temperatures ranging from -2 to ...199514965949
the potential of lolium perenne for revegetation of contaminated soil from a metallurgical site.a greenhouse study was carried out to determine the possibility of using lolium perenne for revegetation of soil from a former ferrous metallurgical plant (naples, south italy) contaminated by cu, pb and zn at levels above current italian regulatory limits. surface soil samples (0-40 cm) from the facility area where raw minerals were disposed (rm1 and rm2), from a nearby unpolluted cultivated soil (c) as control and a 1:3 mixture of the control with the polluted ones (rm1+c and rm2+c) were utili ...200414967498
comparison of tall fescue (cyperales: gramineae) to other cool-season turfgrasses for tolerance to european chafer (coleoptera: scarabaeidae).three cultivars of tall fescue, festuca arundinacea schreb., were compared with three cultivars each of fine fescue (festuca spp.), kentucky bluegrass (poa pratensis l.), and perennial ryegrass (lolium perenne l.) to evaluate tolerance to root-feeding by european chafer grubs, rhizotrogus majalis (razoumowsky). potted turfgrasses were infested with initial densities equivalent to 33 or 66 grubs per 0.1 m2 on 19 august 2000. more grubs were added in late september and october, bringing the total ...200314977131
turfgrass, crop, and weed hosts of blissus occiduus (hemiptera: lygaeidae).blissus occiduus barber is an important pest of buffalograss, buchloë dactyloides (nuttall) engelmann, turf. no-choice studies documented the susceptibility of selected turfgrasses, crops, and weeds to b. occiduus feeding. highly to moderately susceptible grasses included buffalograss; yellow setaria glauca (l.) and green foxtail setaria viridis (l.); kentucky bluegrass, poa pratensis l.; perennial ryegrass, lolium perenne l.; brome, bromus spp. leyss.; zoysiagrass, zoysia japonica steudel; berm ...200414998128
endophyte transmission via seeds of lolium perenne l.: immunodetection of fungal antigens.we traced the presence and distribution of neotyphodium lolii within developing inflorescences and embryos of perennial ryegrass (lolium perenne l.), cultivar "grassland nui," by in situ immunolocalization of fungal proteins. evidence is presented that the fungus penetrates through the rachilla at the base of the ovary, and localizes in a very precise and specific manner in the ovular nucellus, but never enters the embryo sac or the integuments. young embryos do not contain mycelium, but as they ...200415050542
selection of a highly virulent fungal isolate, metarhizium anisopliae clo 53, for controlling hoplia philanthus.the june beetle, hoplia philanthus füessly (coleoptera: scarabaeidae), has become a widespread and destructive insect pest of lawns, sport turf, pastures, and horticultural crops in belgium. the virulence of 34 entomopathogenic fungal isolates from the genera metarhizium, beauveria, and paecilomyces to third-instar h. philanthus was tested in bioassays by dipping larvae in 10(7)conidia/ml suspensions. two isolates of metarhizium anisopliae (clo 53 and clo 54) caused maximally 90% mortality 10 we ...200415050838
fertilizer source effect on ground and surface water quality in drainage from turfgrass.nutrients in surface and ground water can affect human and aquatic organisms that rely on water for consumption and habitat. a mass-balance field study was conducted over two years (july 2000-may 2001) to determine the effect of nutrient source on turfgrass runoff and leachate. treatments were arranged in an incomplete randomized block design on a slope of 7 to 9% of arkport sandy loam (coarseloamy, mixed, active, mesic lamellic hapludalf) and seeded with kentucky bluegrass (poa pratensis l.) an ...200415074817
short-term sequestration of slurry-derived carbon into particle size fractions of a temperate grassland soil.surface application of animal wastes in intensive grassland systems has caused growing environmental problems during the last decade and, therefore, increasing public and scientific concern. in the present study we examined if the natural abundance 13c stable isotope tracer techniques could be used to investigate a poorly defined aspect of waste application, i.e. incorporation of slurry-derived c and its distribution in soil organic matter (som) fractions with different turnover times of a pastu ...200415085987
effect of mecoprop drift on some plant species of conservation interest when grown in standardized mixtures in microcosms.there has been an increasing awareness of potential impacts of herbicide drift on to vegetation in nature reserves and field margin habitats adjacent to treated areas. previous work using single species bioassays has suggested that the effects of a single drift event are confined close to the sprayer (< 10 m). in the present study eight native dicotyledonous species, with and without a perennial grass (lolium perenne), were grown in standardized microcosms in order to study (1) the effects of he ...199115092089
vegetation establishment on a deposit of zinc mine wastes.field trials concerning the establishment of plant cover on a deposit of wastes from the ammeberg zinc mine in central sweden were carried out during 1976-1985. different soil conditioners and manures were applied and plant species cultivars were evaluated with regard to plant biomass, vigour, durability and content of zinc, lead and cadmium. sewage sludge and topsoil led to better establishment of grasses than did municipal waste, straw and hydraulic seeding. after 2 years, festuca rubra and po ...198915092483
effects of ambient levels of air pollution on grass swards subjected to different defoliation regimes.the urgent need to evaluate effects of air quality on plant growth in the field is discussed in relation to the difficulties which field-based studies pose in the interpretation of data. an initial study is described which aimed to overcome some of these difficulties and the data are discussed within the remaining limitations. the productivity of pure swards of lolium perenne l. and dactylis glomerata l., subjected to defoliation at different times of the year, was studied at field sites with di ...198715092760
patterns of soil copper contamination and temporal changes in vegetation in the vicinity of a copper rod rolling factory.patterns of soil copper contamination have been examined in the vicinity of a copper rod rolling plant in prescot, merseyside, uk. the site, established in 1975, was found to possess clear patterns of soil copper contamination, with the highest levels of hno3 and water-extractable fractions encountered in the factory grounds adjacent to the location of the furnace chimney. the majority of the copper had accumulated in the upper soil horizons. the site is surrounded by planted lawns, established ...199715093451
[young perennial ryegrass staggers in a dairy herd].ryegrass staggers was diagnosed in a group of young stock on a dairy farm in the netherlands. the cattle were fed on ryegrass hay originating from a farm in the region. the typical clinical signs observed in this herd were difficulty in rising, hypermetria of especially the front legs, incoordination, and tremors of the head and neck muscles. the morbidity of the illness was about 80 percent and there was no mortality. four weeks after withdrawal of the hay, the animals recovered completely. the ...200415156654
phytoextraction for clean-up of low-level uranium contaminated soil evaluated.spills in the nuclear fuel cycle have led to soil contamination with uranium. in case of small contamination just above release levels, low-cost yet sufficiently efficient remedial measures are recommended. this study was executed to test if low-level u contaminated sandy soil from a nuclear fuel processing site could be phytoextracted in order to attain the required release limits. two soils were tested: a control soil (317 bq 238u kg(-1)) and the same soil washed with bicarbonate (69 bq 238u k ...200415162854
atrazine and simazine degradation in pennisetum rhizosphere.the ability of rhizosphere of four plant species to promote the degradation of charcoal-fixed atrazine and simazine in cement blocks of a long-term contaminated soil when mixed with a normal soil at 1:1 ratio was tested. of the four selected plants viz., rye grass (lolium perenne), tall fescue (festuca arundinacae), pennisetum (pennisetum clandestinum) and a spring onion (allium sp.) used in this study, only p. clandestinum was able to survive in herbicide contaminated soil while other plants di ...200415172598
preliminary ecotoxicological characterization of a new energetic substance, cl-20.a new energetic substance hexanitrohexaazaisowurtzitane (or cl-20) was tested for its toxicities to various ecological receptors. cl-20 (epsilon-polymorph) was amended to soil or deionized water to construct concentration gradients. results of microtox (15-min contact) and 96-h algae growth inhibition tests indicate that cl-20 showed no adverse effects on the bioluminescence of marine bacteria vibrio fischeri and the cell density of freshwater green algae selenastrum capricornutum respectively, ...200415234161
agri-environmental grass hay: nutritive value and intake in comparison with hay from intensively managed grassland.chemical composition, digestibility, nutritive value and intake of hay from an agri-environmental management (eh) were compared with those from hay (lolium perenne) from an intensive management (ih). ih was of low to moderate quality because of unfavourable weather conditions. eh was harvested mid-june of 2000 (eh1) and 2001 (eh2) on the same sward that had not received mineral fertilizer for 10 years. the eh was characterized by a species-rich botanical composition. on average, it had lower con ...200415264672
the effects of lactic acid bacteria inoculants and formic acid on the formation of biogenic amines in grass silages.silages were prepared in six laboratory experiments from four direct-cut grassland swards and pure swards of perennial ryegrass and false oat with dry matter contents ranging between 180 and 325 g/kg. grass was fermented at 22 degrees c and silages were stored at the same temperature for 4 months. untreated silages (negative control) and silages preserved with 3 g/kg of formic acid (positive control) were compared with silages inoculated with commercial strains of lactobacillus plantarum, lactob ...200415264673
dietary preference of dairy cows grazing ryegrass and white clover.the dietary preference of lactating dairy cows grazing perennial ryegrass (lolium perenne l.) and white clover (trifolium repens l.) was studied. twelve groups of 2 lactating, holstein-friesian dairy cows grazed 1.2-ha plots containing conterminal monocultures of clover and grass. half of the groups grazed a plot containing 75% clover and 25% grass (by ground area), with the remaining groups grazing a plot containing 25% clover and 75% grass. the intake rates of clover were higher than those of ...200415290979
the effects of forage provision and group size on the behavior of calves.the effects of providing alternative forages to individual or group-reared calves on their behavior were examined in 2 experiments. in experiment 1, 24 calves were reared in groups of 3 or individually in straw-bedded pens from age 1 to 7 wk. one-half of the calves in each treatment were provided with ad libitum cut perennial ryegrass herbage. grass intakes and time spent eating grass were greater for grouped calves than for individual calves. providing grass reduced concentrate intake of groupe ...200415290985
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