Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
a novel protein elicitor (panie) from pythium aphanidermatum induces multiple defense responses in carrot, arabidopsis, and tobacco.a novel protein elicitor (panie(234)) from pythium aphanidermatum (edson) fitzp. was purified, microsequenced, and the corresponding cdna was cloned. the deduced amino acid sequence contains a putative eukaryotic secretion signal with a proteinase cleavage site. the heterologously expressed elicitor protein without the secretion signal of 21 amino acids (panie(213)) triggered programmed cell death and de novo formation of 4-hydroxybenzoic acid in cultured cells of carrot (daucus carota). program ...200111706166
amelioration of nickel phytotoxicity in muck and mineral soils.in situ remediation (phytostabilization) is a cost-effective solution for restoring the productivity of metal-contaminated soils and protection of food chains. a pot experiment with wheat (triticum aestivum l.), oat (avena sativa l.), and redbeet (beta vulgaris l.) was conducted to test the ability of limestone and hydrous ferric oxide (hfo) to ameliorate ni phytotoxicity in two soils contaminated by particulate emissions from a nickel refinery. quarry muck (terric haplohemist; 72% organic matte ...201511790001
inhibition of gravitropism in oat coleoptiles by the calcium chelator, ethyleneglycol-bis-(beta-aminoethyl ether)-n,n'-tetraacetic acid.a treatment period as brief as 8 h in 10(-3) m egta completely blocks gravitropism in 70-80% of the treated coleoptiles of oats (avena sativa l. cv. garry) without inhibiting growth. only about 10% of the plants perfused in water failed to exhibit gravitropism. subsequent perfusion of egta-treated plants with calcium completely restores gravitropism; post-perfusion with water does not. after perfusion in water for 10 h, gravistimulated oat coleoptile segments show the same asymmetry of 45ca dist ...198411829020
[kinetics of mitotic rhythmic activity in oat (avena sativa) coleoptiles following irradiation].the analysis of different intensity (10-200 gy) irradiation influence on rhythmical processes in mitotic activity of oats coleoptiles was carries out. obtained results indicate that irradiation disorders phase and amplitude of the cell division rhythm in investigated material. this disturbance, as judged by doze dependence, is associated with unknown molecular structures.201511833332
modulating effects of oatmeal extracts in the sodium lauryl sulfate skin irritancy model.the aim of the present study was to assess the anti-inflammatory activity of two topically applied oatmeal extracts, i.e. avena sativa and avena rhealba, using the sodium lauryl sulfate (sls) irritation model. at baseline, test areas on the volar surface of the upper arms of 12 healthy individuals were pretreated with the two extracts and their vehicle (petrolatum ointment) under occlusion for 2 h, and one site was left untreated. then a patch with a 1% sls solution was applied to the test sites ...201511867969
effects of commercial processing on levels of antioxidants in oats (avena sativa l.).the effects of various commercial hydrothermal processes (steaming, autoclaving, and drum drying) on levels of selected oat antioxidants were investigated. steaming and flaking of dehulled oat groats resulted in moderate losses of tocotrienols, caffeic acid, and the avenanthramide bp (n-(4'-hydroxy)-(e)-cinnamoyl-5-hydroxy-anthranilic acid), while ferulic acid and vanillin increased. the tocopherols and the avenanthramides bc (n-(3',4'-dihydroxy-(e)-cinnamoyl-5-hydroxy-anthranilic acid) and bf ( ...200211902929
genetic and physical mapping of lrk10-like receptor kinase sequences in hexaploid oat (avena sativa l.).oat receptor-like kinase gene sequences, homologous to the lrk10 gene from wheat (triticum aestivum l.), were mapped in oat (avena sativa l.). pcr primers designed from the wheat lrk10 were used to produce alrk10 from oat. two dna sequences, alrk1a1 and alrk4a5, were produced from primers designed from coding and noncoding regions of alrk10. their use as rflp probes indicated that the kinase genes mapped to four loci on different hexaploid oat 'kanota' x 'ogle' linkage groups (4_12, 5, 6, and 13 ...200211908651
chemistry and biology of phototropism-regulating substances in higher plants.most people are familiar with the sight of a young seedling bending towards a window or the brightest source of light to which it is exposed. this directional growth response is known as phototropism, which is caused by a lateral growth-promoting auxin in the bending organ (cholodny-went theory, cited in high school textbook). recently, however, bruinsma et al., weiler et al., and hasegawa et al. independently found that the shaded half did not contain more auxin than the illuminated one. instea ...200111933243
isolation of a/d and c genome specific dispersed and clustered repetitive dna sequences from avena sativa.dna gel-blot and in situ hybridization with genome-specific repeated sequences have proven to be valuable tools in analyzing genome structure and relationships in species with complex allopolyploid genomes such as hexaploid oat (avena sativa l., 2n = 6x = 42; aaccdd genome). in this report, we describe a systematic approach for isolating genome-, chromosome-, and region-specific repeated and low-copy dna sequences from oat that can presumably be applied to any complex genome species. genome-spec ...200211962640
ethanol sensitivity of rice and oat coleoptiles.the ability to avoid the ethanol-induced injury was evaluated in rice (oryza sativa l.) and oat (avena sativa l.) coleoptiles. the growth of the rice and oat coleoptiles was inhibited by ethanol exogenously applied at concentrations greater than 200 and 30 mm, respectively. at 300 mm ethanol, oat coleoptiles were brown and flaccid but rice coleoptiles did not show any visible symptoms of toxicity. the acetaldehyde level in rice and oat coleoptiles was increased by exogenously applied ethanol and ...200212010475
repetition of the classical boysen-jensen and nielsen's experiment on phototropism of oat coleoptiles.the classical experiment of phototropic response as reported by boysen-jensen and nielsen (1926), which supports the cholodny-went theory, was repeated in detail. in the original experiment, etiolated oat (avena sativa l. cv. victory) coleoptiles with mica inserted into their tip only showed a positive response when the mica was placed parallel toward the light source and not if it was inserted perpendicularly. on the contrary, we found a positive response irrespective of whether the mica was in ...200012090269
the serine-rich n-terminal domain of oat phytochrome a helps regulate light responses and subnuclear localization of the photoreceptor.phytochrome (phy) a mediates two distinct photobiological responses in plants: the very-low-fluence response (vlfr), which can be saturated by short pulses of very-low-fluence light, and the high-irradiance response (hir), which requires prolonged irradiation with higher fluences of far-red light (fr). to investigate whether the vlfr and hir involve different domains within the phya molecule, transgenic tobacco (nicotiana tabacum cv xanthi) and arabidopsis seedlings expressing full-length (fl) a ...200212114567
toxicity of methyl tert-butyl ether to plants (avena sativa, zea mays, triticum aestivum, and lactuca sativa).influence of methyl tert-butyl ether (mtbe) on the germination of seeds and growth of seedling plants were studied in laboratory experiments. test plants were wild oats (avena sativa), sweet corn (zea mays), wheat (triticum aestivum), and lettuce (lactuca sativa). seed germination, shoot growth, and root growth of plants exposed to different concentrations of mtbe in a moist soil were examined. seed germination and seedling growth in mtbe-contaminated soil were markedly reduced in all test plant ...200212152769
independent activation of cold acclimation by low temperature and short photoperiod in hybrid aspen.temperate zone woody plants cold acclimate in response to both short daylength (sd) and low temperature (lt). we were able to show that these two environmental cues induce cold acclimation independently by comparing the wild type (wt) and the transgenic hybrid aspen (populus tremula x populus tremuloides michx.) line 22 overexpressing the oat (avena sativa) phytochrome a gene. line 22 was not able to detect the sd and, consequently, did not stop growing in sd conditions. this resulted in an impa ...200212177476
phosphoryl group exchange between atp and adp catalyzed by h+-atpase from oat roots.atp-adp exchange was estimated in the presence of plasma membrane h+-atpase of oat (avena sativa) roots partially purified with triton x-100 by measuring [14c]atp formation from [14c]adp. most studies were done at 0[deg]c. at ph 6.0 the exchange showed: (a) mg2+ requirement with a biphasic response giving maximal activity at 152 [mu]m and (b) insensitivity to ionic strength, [na+], and [k+]. atp and adp dependence were analyzed with a model in which nucleotide-enzyme interactions are at rapid-ra ...199712223778
peroxygenase-catalyzed fatty acid epoxidation in cereal seeds (sequential oxidation of linoleic acid into 9(s),12(s),13(s)-trihydroxy-10(e)-octadecenoic acid).peroxygenase-catalyzed epoxidation of oleic acid in preparations of cereal seeds was investigated. the 105,000g particle fraction of oat (avena sativa) seed homogenate showed high peroxygenase activity, i.e. 3034 [plus or minus] 288 and 2441 [plus or minus] 168 nmol (10 min)-1 mg-1 protein in two cultivars, whereas the corresponding fraction obtained from barley (hordeum vulgare and hordeum distichum), rye (secale cereale), and wheat (triticum aestivum) showed only weak activity, i.e. 13 to 138 ...199612226220
osmoregulation by oat coleoptile protoplasts (effect of auxin).the effect of auxin on the physiology of protoplasts from growing oat (avena sativa l.) coleoptiles was investigated. protoplasts, isolated iso-osmotically from peeled oat coleoptile segments, were found to swell steadily over many hours. incubated in 1 mm cacl2, 10 mm kcl, 10 mm 2-(morpholino)ethanesulfonic acid/1,3-bis-[tris(hydroxymethyl)methylamino]propane, ph 6.5, and mannitol to 300 milliosmolal, protoplasts swelled 28.9% [plus or minus] 2.0 (standard error) after 6 h. addition of 10 [mu]m ...199612226237
mgatp-dependent transport of phytochelatins across the tonoplast of oat roots.in cd-exposed oat (avena sativa) roots cd was found to be associated primarily with the phytochelatin ([gamma]-glutamylcysteinyl)3-glutamic acid [([gamma]ec)3g], with a peptide to cd ratio of 1:3 (cysteine to cd ratio of 1:1), even though both ([gamma]ec)2g and ([gamma]ec)3g were present in the roots. phytochelatins are known to accumulate in the vacuoles of plant cells on exposure to cd, but the mechanism is not clear. here we present evidence for the transport of the phytochelatins ([gamma]ec) ...199512228436
arginine decarboxylase is localized in chloroplasts.plants, unlike animals, can use either ornithine decarboxylase or arginine decarboxylase (adc) to produce the polyamine precursor putrescine. lack of knowledge of the exact cellular and subcellular location of these enzymes has been one of the main obstacles to our understanding of the biological role of polyamines in plants. we have generated polyclonal antibodies to oat (avena sativa l.) adc to study the spatial distribution and subcellular localization of adc protein in different oat tissues. ...199512228631
calcium antagonists inhibit sustained gibberellic acid-induced growth of avena (oat) stem segments.the elongation response of avena sativa (oat) stem segments to gibberellic acid (ga3) is of large magnitude, with high hormonal sensitivity and specificity, but without cell division activity. this system is therefore an excellent model for mechanistic studies on higher plant cell elongation and the action of gibberellin. at millimolar concentrations, the calcium antagonists verapamil, d-600, nicardipine, diltiazem, bepridil, 8-(n,n,-diethylamino)-octyl-3,4,5-trimethoxybenzoate hcl, and lanthanu ...199312231695
two related biosynthetic pathways of mugineic acids in gramineous plants.the biosynthesis of mugineic acids was studied by feeding 2h- or 13c-labeled compounds to water-cultured roots in several gramineous plants. the fate of labeled compounds was monitored by using 2h- and 13c-nuclear magnetic resonance. on investigating the proton changes during biosynthesis by feeding d,l-[3,3,4,4-d4]-methionine (98.6% 2h), 2h-labeled 2[prime]-deoxymugineic, mugineic, and 3-epihydroxymugineic acids were isolated from root washings of wheat (triticum aestivum l. cv minori), barley ...199312231828
evidence for two indoleacetic acid-induced growth responses in the avena straight-growth indoleacetic acid assay.floating avena sativa l. cv victory coleoptile segments were used to determine whether the straight-growth indoleacetic acid (iaa) assay can be reconciled with the avena curvature assay and the cholodny-went theory of photo- and gravitropism. measurements of segment length after 5 h yield sigmoid-shaped iaa dose-response curves with the growth rate leveling off at 1 [mu]m. however, measurements made at 24 h generate bell-shaped curves with maximal growth being induced by 10 [mu]m iaa. the differ ...199312231831
density gradient study of victorin-binding proteins in oat (avena sativa) cells.victorin-binding proteins (vbps) in oat (avena sativa) cells were identified using native victorin and anti-victorin polyclonal antibodies. homogenates of oat tissues were fractionated in continuous or discontinuous sucrose density gradients or with an aqueous two-phase method, and covalent binding sites of victorin were detected by western blotting. in a 20 to 45% (w/w) sucrose continuous density gradient, the 100-kd vbp was located in fractions of 37 to 44% sucrose, with a peak at 39% sucrose. ...199312231915
determination of auxin-dependent ph changes in coleoptile cell walls by a null-point method.the present debate on the validity of the "acid-growth theory" of auxin (indole-3-acetic acid, iaa) action concentrates on the question of whether iaa-induced proton excretion into the cell wall is quantitatively sufficient to provide the shift in ph that is required to explain iaa-induced growth (see d.l. rayle, r.e. cleland [1992] plant physiol 99:1271-1274 for a recent apologetic review of the acid-growth theory). in the present paper a null-point method has been employed for determining the ...199312231942
role of expansin in cell enlargement of oat coleoptiles (analysis of developmental gradients and photocontrol).expansins are wall proteins that mediate a type of acid-induced extension in isolated plant cell walls (s. mcqueen-mason, d.m. durachko, d.j. cosgrove [1992] plant cell 4: 1425-1433). to assess the role of these proteins in the process of cell enlargement in living tissues, we compared the spatial and temporal growth patterns of oat (avena sativa l.) coleoptiles with four wall properties related to expansin action. these properties were (a) the ability of isolated walls and living segments to ex ...199312232025
a comparison of freezing injury in oat and rye: two cereals at the extremes of freezing tolerance.a detailed analysis of cold acclimation of a winter rye (secale cereale l. cv puma), a winter oat (avena sativa l. cv kanota), and a spring oat cultivar (ogle) revealed that freezing injury of leaves of nonacclimated seedlings occurred at -2[deg]c in both the winter and spring cultivars of oat but did not occur in winter rye leaves until after freezing at -4[deg]c. the maximum freezing tolerance was attained in all cultivars after 4 weeks of cold acclimation, and the temperature at which 50% ele ...199412232096
a contrast of the plasma membrane lipid composition of oat and rye leaves in relation to freezing tolerance.the lipid composition of the plasma membrane isolated from leaves of spring oat (avena sativa l. cv ogle) was vastly different from that of winter rye (secale cereale l. cv puma). the plasma membrane of spring oat contained large proportions of phospholipids (28.8 mol% of the total lipids), cerebrosides (27.2 mol%), and acylated sterylglucosides (27.3 mol%) with lesser proportions of free sterols (8.4 mol%) and sterylglucosides (5.6 mol%). in contrast, the plasma membrane of winter rye contained ...199412232097
modulation of h+-atpase activity by fusicoccin in plasma membrane vesicles from oat (avena sativa l.) roots (a comparison of modulation by fusicoccin, trypsin, and lysophosphatidylcholine).the fungal phytotoxin fusicoccin affects various transport processes in the plasma membrane of plant cells. the plasma membrane (pm) h+-atpase (ec 3.6.1.35) seems to be the primary target of fusicoccin action. the kinetics of the stimulation of the pm h+-atpase by fusicoccin was studied in pm vesicles isolated from oat (avena sativa cv adamo) roots by aqueous two-phase partitioning. considerable stimulation of activity was observed only when roots were treated with fusicoccin prior to the pm iso ...199412232167
purification of a membrane-bound udp-glucose:sterol [beta]-d-glucosyltransferase based on its solubility in diethyl ether.membrane-bound udp-glucose:sterol [beta]-d-glucosyltransferase (udpg-sgtase) catalyzes the formation of steryl glucosides from udp-glucose and free sterols. this enzyme was purified from etiolated oat shoots (avena sativa l. cv alfred) in five steps. udpg-sgtase was solubilized from a microsomal fraction with the detergent n-octyl-[beta]-d-thioglucopyranoside and then extracted into diethyl ether. subsequent removal of the organic solvent, resolubilization with an aqueous buffer, and two column ...199412232266
purification of the fusicoccin-binding protein from oat root plasma membrane by affinity chromatography with biotinylated fusicoccin.fusicoccin (fc), a fungal phytotoxin, evokes a number of physiological responses after binding to the fc-binding protein (fcbp). for characterization of this plasma membrane protein and elucidation of the signal transduction pathway, we purified active fcbp from oat (avena sativa l. cv valiant) root plasma membranes using avidin-biotin affinity chromatography. for the binding of fcbp to immobilized avidin, a bifunctional fc derivative (fc-biotin, fcbio) was synthesized. fcbio retained high bindi ...199412232284
tropisms of avena coleoptiles: sine law for gravitropism, exponential law for photogravitropic equilibrium.the quantitative relation between gravitropism and phototropism was analyzed for light-grown coleoptiles of avena sativa (l.). with respect to gravitropism the coleoptiles obeyed the sine law. to study the interaction between light and gravity, coleoptiles were inclined at variable angles and irradiated for 7 h with unilateral blue light (466 nm) impinging at right angles relative to the axis of the coleoptile. the phototropic stimulus was applied from the side opposite to the direction of gravi ...200212244443
serpins of oat (avena sativa) grain with distinct reactive centres and inhibitory specificity.most proteinase inhibitors from plant seeds are assumed to contribute to broad-spectrum protection against pests and pathogens. in oat (avena sativa l.) grain the main serine proteinase inhibitors were found to be serpins, which utilize a unique mechanism of irreversible inhibition. four distinct inhibitors of the serpin superfamily were detected by native page as major seed albumins and purified by thiophilic adsorption and anion exchange chromatography. the four serpins osza-d are the first pr ...200212354191
direct capture and cloning of receptor kinase and peroxidase genes from genomic dna.a direct dna capture and cloning procedure with magnetic bead separation was used to isolate receptor kinase like and peroxidase genes from oat (avena sativa) and wheat (triticum aestivum l.) genomic dna, respectively. in this procedure, the digoxigenin-labeled probe dna and target genomic dna fragments were mixed, denatured, and hybridized. the double-helix complexes formed were captured with anti-digoxigenin immunoglobulin-coated magnetic beads and then cloned into either the lambdabluestar or ...200212416632
complex transgene locus structures implicate multiple mechanisms for plant transgene rearrangement.to more fully characterize the internal structure of transgene loci and to gain further understanding of mechanisms of transgene locus formation, we sequenced more than 160 kb of complex transgene loci in two unrelated transgenic oat (avena sativa l.) lines transformed using microprojectile bombardment. the transgene locus sequences from both lines exhibited extreme scrambling of non-contiguous transgene and genomic fragments recombined via illegitimate recombination. a perfect direct repeat of ...200212445116
gramene, a tool for grass genomics.gramene (http://www.gramene.org) is a comparative genome mapping database for grasses and a community resource for rice (oryza sativa). it combines a semi-automatically generated database of cereal genomic and expressed sequence tag sequences, genetic maps, map relations, and publications, with a curated database of rice mutants (genes and alleles), molecular markers, and proteins. gramene curators read and extract detailed information from published sources, summarize that information in a stru ...200212481044
fluorescence in situ hybridization mapping of avena sativa l. cv. sunii and its monosomic lines using cloned repetitive dna sequences.fluorescent in situ hybridization (fish) employing multiple probes was used with mitotic or meiotic chromosome spreads of avena sativa l. cv. sunii and its monosomic lines to produce physical chromosome maps. the probes used were avena strigosa pas120a (which hybridizes exclusively to a-genome chromosomes), avena murphyi pam1 (which hybridizes exclusively to c-genome chromosomes), a. strigosa pas121 (which hybridizes exclusively to a- and d-genome chromosomes), and the wheat rdna probes pta71 an ...200212502269
characterization of two phases of chlorophyll formation during greening of etiolated barley leaves.the esterification kinetics of chlorophyllide, obtained by a single flash of light, were investigated in etiolated barley ( hordeum vulgare l.) and oat ( avena sativa l.) leaves. a rapid phase, leading to esterification of 15% of total chlorophyllide within 15-30 s, was followed by a lag-phase of nearly 2 min and a subsequent main phase, leading to esterification of 85% of total chlorophyllide within 30-60 min. the presence of additional protochlorophyllide, produced in the leaves by incubation ...200312520340
blue light perception in plants. detection and characterization of a light-induced neutral flavin radical in a c450a mutant of phototropin.the lov2 domain of avena sativa phototropin and its c450a mutant were expressed as recombinant fusion proteins and were examined by optical spectroscopy, electron paramagnetic resonance, and electron-nuclear double resonance. upon irradiation (420-480 nm), the lov2 c450a mutant protein gave an optical absorption spectrum characteristic of a flavin radical even in the absence of exogenous electron donors, thus demonstrating that the flavin mononucleotide (fmn) cofactor in its photogenerated tripl ...200312525505
pre-loading of chlorophyll synthase with tetraprenyl diphosphate is an obligatory step in chlorophyll biosynthesis.the reaction of recombinant chlorophyll synthase from avena sativa, expressed in escherichia coli, was investigated. to verify the identity of the recombinant and native enzymes, reaction rates were determined for both enzyme preparations with several chlorophyllide analogs. the rates of esterification of these modified substrates ranged from 0 to 100% of the rate with the natural substrate, and were nearly identical for both enzyme preparations. the lineweaver-burk plot for variation of both ch ...200212530542
avenanthramides in oats (avena sativa l.) and structure-antioxidant activity relationships.eight avenanthramides, amides of anthranilic acid (1) and 5-hydroxyanthranilic acid (2), respectively, and the four cinnamic acids p-coumaric (p), caffeic (c), ferulic (f), and sinapic (s) acid, were synthesized for identification in oat extracts and for structure-antioxidant activity studies. three compounds (2p, 2c, and 2f) were found in oat extracts. as assessed by the reactivity toward 1,1-diphenyl-2-picrylhydrazyl (dpph), all avenanthramides except 1p showed activity. initially, the antioxi ...200312537428
purification and properties of aminoaldehyde dehydrogenase from avena sativa.nad-dependent aminoaldehyde dehydrogenase (amadh, ec 1.2.1.-) from avena shoots was purified by deae sephacel, hydroxyapatite, 5'-amp sepharose 4b, mono q, and tsk-gel column chromatographies to homogeneity by the criterion of native page. sds-page yielded a single band at a molecular mass of 55 kda. ief studies showed a band with a p i value of 5.3. in contrast to amadhs from other species, the tsk-gel chromatography showed that avenaamadh exists as a monomer in the native state. the purified e ...200212579364
competence of oat ( avena sativa l.) shoot apical meristems for integrative transformation, inherited expression, and osmotic tolerance of transgenic lines containing hva1.three oat ( avena sativa l.) cultivars have been successfully transformed using an efficient and reproducible in vitro culture system for differentiation of multiple shoots from shoot apical meristems. the transformation was performed using microprojectile bombardment with two plasmids (pby520 and pact1-d) containing linked ( hva1-bar) and non-linked ( gus) genes. the hva1 and bar genes cointegrated with a frequency of 100% as expected, and 61.6% of the transgenic plants carried all three genes. ...200212582520
changes in phosphorylation of 50 and 53 kda soluble proteins in graviresponding oat (avena sativa) shoots.the present work indicates that phosphorylation of a 50 kda soluble protein is involved in the gravitropic response in graviresponsive pulvini of oat (avena sativa) stems. this 50 kda protein shows a differential pattern of phosphorylation between lower and upper halves of pulvini both in vivo and in vitro. the differential phosphorylation of this protein is detected only when stem segments are gravistimulated for short and long time periods. the differential phosphorylation of the 50 kda protei ...200312598571
growth performance, carcass and pork quality of finisher pigs fed oat-based diets containing different levels of beta-glucans.a study was undertaken to investigate the growth performance and carcass and meat quality of pigs (bw = 52 to 108 kg) fed oat-based (avena sativa l.) diets containing four levels of mixed-linkage (1 --> 3), (1 --> 4)-beta-d-glucans. one hundred sixty pigs-80 barrows and 80 gilts (average starting bw = 52.7 kg)--were allocated to one of five diets: a wheat-barley-based control diet and four experimental diets. the groats of marion, a covered oat, and ot789, a hulless oat, were used to formulate f ...200312643489
primary reactions of the lov2 domain of phototropin, a plant blue-light photoreceptor.the phototropins constitute an important class of plant photoreceptor kinases that control a range of physiological responses, including phototropism, light-directed chloroplast movement, and light-induced stomatal opening. the lov2 domain of phototropin binds a molecule of flavin mononucleotide (fmn) and undergoes a photocycle involving light-driven covalent adduct formation between a conserved cysteine residue and the c(4a) atom of fmn. this product state promotes c-terminal kinase activation ...200312653541
a molecular marker map in 'kanota' x 'ogle' hexaploid oat (avena spp.) enhanced by additional markers and a robust framework.molecular mapping of cultivated oats was conducted to update the previous reference map constructed using a recombinant inbred (ri) population derived from avena byzantina c. koch cv. kanota x avena sativa l. cv. ogle. in the current work, 607 new markers were scored, many on a larger set of ri lines (133 vs. 71) than previously reported. a robust, updated framework map was developed to resolve linkage associations among 286 markers. the remaining 880 markers were placed individually within the ...200312669794
isolation and identification of triticeae chromosome 1 receptor-like kinase genes (lrk10) from diploid, tetraploid, and hexaploid species of the genus avena.the dna sequence of an extracellular (exc) domain of an oat (avena sativa l.) receptor-like kinase (alrk10) gene was amplified from 23 accessions of 15 avena species (6 diploid, 6 tetraploid, and 3 hexaploid). primers were designed from one partial oat alrk10 clone that had been used to map the gene in hexaploid oat to linkage groups syntenic to triticeae chromosome 1 and 3. cluster (phylogenetic) analyses showed that all of the oat dna sequences amplified with these primers are orthologous to t ...200312669804
comparison of cytokinin-binding proteins from wheat and oat grains.cytokinin-binding proteins (cbps) isolated from mature grains of oat (avena sativa l.) and wheat (triticum aestivum l.) by acid precipitation, ion-exchange and affinity chromatography had similar characteristics, although they differed somewhat in apparent molecular weight of the native protein as determined by gel filtration (109 and 133 kda, respectively) and subunit size as estimated by sds-polyacrylamide gel electrophoresis (47 and 55 kda, respectively). highly purified oat cbp showed very w ...200312675735
mapping of low- and high-fluence autophosphorylation sites in phototropin 1.phototropins, originally detected by their blue light-dependent autophosphorylation, are plant photoreceptors involved in several blue light responses such as phototropism, chloroplast relocation, leaf expansion, rapid inhibition of hypocotyl growth, and stomatal opening. three domains have been identified in phototropin sequences, two chromophore binding domains (lov1 and lov2) and a kinase domain. we describe here two additional domains, the n-terminus upstream of lov1 and the hinge region bet ...200312680776
inhibitory effect of sejin-eum i/ii on nicotine- and cigarette extract-induced cytotoxicity in human lung fibroblast.nicotine is a major pharmacologically active component of cigarette smoke. excessive cigarette smoking is harmful to lung. sejin-eum (sje) i is composed of various oriental medicines, and sje ii is sje i plus seeds of avena sativa (gramineae) that reduces the craving for cigarette in man. in this study, we have examined whether an aqueous extract of sje i/ii inhibits nicotine- or cigarette extract (ce)-induced cytotoxicity in human embryonic lung fibroblast, mrc-9. assessment of cell viability u ...200312686436
"chitin-specific" peroxidases in plants.the activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. some increase in peroxidase activity was observed in crude extracts in the presence of chitin. activated peroxidases of some species fell in the fraction not sorbed on chitin and those of other species can bind chitin. only anionic isoperoxidases from oat (avena sativa), rice (oryza sativa), horseradish (armoracia rusticana), garden radish (raphanus sativus var. radicula), peanut (a ...200312693984
cadmium contents of oats (avena sativa l.) in official variety, organic cultivation, and nitrogen fertilization trials during 1997-1999.the cadmium (cd) contents of oats (avena sativa l.) in finland were examined over a 3 year period in three types of trials: official variety, organic vs conventional cultivation, and nitrogen fertilization trials. large seasonal and regional variations were found in the cd concentrations. in official variety trials, the mean cd contents in 1997, 1998, and 1999 were 0.046, 0.029, and 0.052 mg kg(-1) dry weight (dw), respectively, ranging from 0.008 to 0.120 mg kg(-1) dw. the concentrations were g ...200312696945
apatite and phillipsite as sequestering agents for metals and radionuclides.laboratory and greenhouse studies were conducted to quantify apatite and phillipsite (zeolite) sequestration of selected metal contaminants. the laboratory batch study measured the sorption of aqueous co2+, ba2+, pb2+, eu3+, and uo2(2+). apatite sorbed more co2+, pb2+, eu3+, and uo2(2+) from the spike solution than phillipsite, resulting in distribution coefficients (kd values) of >200,000 l kg(-1). phillipsite was more effective than apatite at sorbing aqueous ba2+. results from the laboratory ...201512708675
mechanism of physiological effects of titanium leaf sprays on plants grown on soil.titanium (ti) has significant biological effects on plants, being beneficial at low and toxic at higher concentrations. from results of our hydroponical experiment with oats, we have recently proposed that the effect called hormesis is the mechanism of ti action in plants. here, we present the experiment with oats (avena sativa l. cv. zlat'ák) grown on soil where ti was applied using leaf sprays. two different soils, three different concentrations of ti(iv) citrate spray solution (0, 20, and 50 ...200312719613
a genetic linkage map for hexaploid, cultivated oat (avena sativa l.) based on an intraspecific cross 'ogle/mam17-5'.genetic research and breeding of oat ( avena sativa l.) would be aided by development of a genetic linkage map for a breeding population. such a map could be used for localization of qualitative and quantitative trait loci, marker-assisted selection and other genetic analysis in an adapted, agronomically useful background. the objectives of this research were to develop a genetic linkage map of hexaploid cultivated oat, to identify homoeologous relationships of linkage groups, and to compare hom ...200312721634
replacement value of undecorticated sunflower meal as a supplement for milk production by crossbred cows and buffaloes in the northern plains of india.the replacement value of undecorticated sunflower meal (sfm) in the diets of dairy animals was assessed on-station and on-farm. eighteen primiparous crossbred (bos taurus x bos indicus) cows (350.4 +/- 8.84 kg), randomly allocated to three groups, were used in the on-station study. the animals were fed on either a conventional concentrate supplement (control) or on an experimental concentrate, in which sfm replaced 25% (sfm-25) or 50% (sfm-50) of the cp in the control supplement. green oats (ave ...200312735704
purification and properties of betaine aldehyde dehydrogenase from avena sativa.betaine aldehyde dehydrogenase (badh; ec 1.2.1.8) is the enzyme that catalyzes the second step in the synthesis of the osmoprotectant, glycine betaine. nad-dependent badh was purified from avena sativa shoots by deae sephacel, hydroxyapatite, 5'-amp sepharose 4b, mono q and tsk-gel column chromatographies to homogeneity by the criterion of native page, and the properties of badh were compared with those of aminoaldehyde dehydrogenase purified to homogeneity from a. sativa. the molecular mass est ...200312736784
cys/gly-rich proteins with a putative single chitin-binding domain from oat (avena sativa) seeds.through a reliable and repeatable procedure based on solid-phase extraction techniques, a protein fraction (p fraction) rich in cys/gly residues was extracted and captured from oat (avena sativa l.) seeds. quantitative amino acid analysis and ms of the p fraction indicated that it contains a series of heterogeneous cys/gly-rich proteins with molecular masses of 3.6-4.0 kda. preliminary results from bioassays showed that these proteins possess weak to moderate antifungal properties to some fungal ...200312737975
molecular mapping of genomic regions underlying barley yellow dwarf tolerance in cultivated oat (avena sativa l.).barley yellow dwarf (byd) is one of the most important viral diseases in small grains, including oat (avena sativa l.). breeding for byd tolerance is an effective and efficient means to control the disease. characterization of major sources of tolerance, and identification of marker and the trait associations, will directly benefit breeding for byd tolerance. genomic regions underlying byd tolerance were mapped and characterized in an oat population consisting of 152 recombinant inbred lines fro ...200312748782
in vivo and in vitro effects of cadmium on h+ atpase activity of plasma membrane vesicles from oat (avena sativa l.) roots.the effect of an in vivo and in vitro treatment with cadmium on transport activities of root plasma membrane enriched vesicles was studied in oat (avena sativa l. cv. argentina) plants. addition of 100 mumol/l cdso4 to nutrient solution decreases both proton transport activity and atpase activity to the same level. in vitro experiments show that cadmium seems to have a differential inhibiting effect on proton transport activity and atpase activity, the most pronounced one on atp-dependent h(+)-a ...200312756918
expression of green fluorescent protein and its inheritance in transgenic oat plants generated from shoot meristematic cultures.the expression of green fluorescent protein (gfp) and its inheritance were studied in transgenic oat ( avena sativa l.) plants transformed with a synthetic green fluorescent protein gene [sgfp(s65t)] driven by a rice actin promoter. in vitro shoot meristematic cultures (smcs) induced from shoot apices of germinating mature seeds of a commercial oat cultivar, garry, were used as a transformation target. proliferating smcs were bombarded with a mixture of plasmids containing the sgfp(s65t) gene an ...200312789450
the development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa.although universal or consensus chloroplast primers are available, they are limited by their number and genomic distribution. therefore, a set of consensus chloroplast primer pairs for simple sequence repeats (ccssrs) analysis was constructed from tobacco (nicotiana tabacum l.) chloroplast sequences. these were then tested for their general utility in the genetic analysis of a diverse array of plant taxa. in order to increase the number of ccssrs beyond that previously reported, the target seque ...200312827249
captures of western corn rootworm (coleoptera: chrysomelidae) adults with pherocon am and vial traps in four crops in east central illinois.it is hypothesized that the long-term rotation of maize (zea mays l.) and soybean (glycine max l.) in east central illinois has caused a significant change in the ovipositional behavior of the western corn rootworm, diabrotica virgifera virgifera leconte. since the mid 1990s in east central illinois, western corn rootworm adults have been observed feeding on soybean foliage and also now use soybean fields as egg laying sites. this behavioral adaptation has greatly decreased the effectiveness of ...200312852611
growth promotion and an increase in cell wall extensibility by silicon in rice and some other poaceae seedlings.the effect of silicon on organ growth and its mechanisms of action were studied in rice ( oryza sativa l. cv. koshihikari), oat ( avena sativa l. cv. victory), and wheat ( triticum aestivum l. cv. daichino-minori) seedlings grown in the dark. applying silicon in the form of silicic acid to these seedlings via culture solution resulted in growth promotion of third (rice) or second (oat and wheat) leaves. the optimal concentration of silicon was 5-10 mm. no growth promotion was observed in early o ...200212884045
reduction in the endogenous arginine decarboxylase transcript levels in rice leads to depletion of the putrescine and spermidine pools with no concomitant changes in the expression of downstream genes in the polyamine biosynthetic pathway.we investigated whether down-regulation of arginine decarboxylase (adc) activity and concomitant changes in polyamine levels result in changes in the expression of downstream genes in the polyamine pathway. we generated transgenic rice (oryza sativa l.) plants in which the rice adc gene was down-regulated by expression of its antisense oat (avena sativa l.) ortholog. plants expressed the oat mrna adc transcript at different levels. the endogenous transcript was down-regulated in five out of eigh ...200312898254
integration, expression and inheritance of transgenes in hexaploid oat (avena sativa l.).two oat varieties, melys (spring variety) and bulwark (winter variety) were transformed by particle bombardment of primary embryogenic callus using either a ubi-bar-ubi-gus co-integration vector or co-transformed (melys) with a ubi-bar plasmid together with one of three plasmids containing the beta-glucuronidase (gus) gene under the control of either a rice actin promoter, a camv35s promoter or a wheat high molecular weight glutenin promoter. morphologically normal and fertile transgenic plants ...200312964869
development of a monoclonal antibody-based enzyme-linked immunosorbent assay to quantify soluble beta-glucans in oats and barley.a set of 31 murine monoclonal antibodies was produced against (1-->3,1-->4)beta-d-glucan from oats (avena sativa l.) chemically cross-linked to keyhole limpet hemocyanin. monoclonal antibodies were tested for their cross-reactivity to related and unrelated polysaccharides. the antibodies reacted strongly to unmodified beta-glucan from oats and barley (hordeum vulgare l.) and to lichenan from icelandic moss, a polysaccharide with a structure similar to that of beta-glucan but which is not encount ...200313129289
[common oat (avena sativa) as a substitute for meat in simple and combined culture media]. 201513136943
[effect of microorganismal culture filtrate on production of scopoletin by germinal roots of oat (avena sativa l. )]. 195813534431
avenacin, an antimicrobial substance isolated from avena sativa. i. isolation and antimicrobial activity. 196414155108
avenacin, an antimicrobial substance isolated from avena sativa. ii. structure. 196414155109
the fine structure of chloroplast stroma following aldehyde osmium-tetroxide fixation.markedly improved fixation of leaf tissues is obtained by means of a glutaraldehyde (or acrolein)-osmium tetroxide procedure, as compared with the results of potassium permanganate or osmium tetroxide fixation methods. the procedure has proved useful in all species so far examined. chloroplasts are particularly well preserved. in this paper details of components of the ground-substance of avena sativa plastids are presented. they include the following:-(i) the "tromacentre" is an area of aggrega ...196514286298
studies on the effects of gaseous ions on plant growth. i. the influence of positive and negative air ions on the growth of avena sativa.exposure of avena sativa seedlings to unipolar ionized atmospheres of either charge produced statistically significant stimulation of growth as measured by mean stem length, integral elongation, and dry weight. the extent of growth increase was related to the atmospheric ion density and this in turn determined the magnitude of current flow to ground. the minimal current measured in the ground circuit and capable of producing a measurable difference in growth was 4.3 to 4.6 x 10(-13) amp/plant.196214459883
damage potential of grasshoppers (orthoptera: acrididae) on early growth stages of small-grains and canola under subarctic conditions.we characterized the type and extent of grasshopper injury to above- and below-ground plant parts for four crops [barley (hordeum vulgare l.), oats (avena sativa l.), wheat (triticum aestivum l.), and canola (brassica campestris l.)] commonly grown, or with potential to grow, in central alaska. cages were placed on 48 pots containing plants in second to third leaf stages and stocked with 0, 2, 4, and 6 first-instar melanoplus sanguinipes f. pot(-1). plants were harvested 22 d after planting. ste ...200314503591
possible evidence for transport of an iron cyanide complex by plants.barley (hordeum vulgare l.), oat (avena sativa l.), and wild cane (sorghum bicolor l.), were exposed to 15n-labeled ferrocyanide to determine whether these plant species can transport this iron cyanide complex. plants were treated with ferrocyanide in a nutrient solution that simulated iron cyanide contaminated groundwater and soil solutions. this nutrient solution has been shown to maintain ferrocyanide speciation with minimal dissociation to free cyanide. following treatment, all three plants ...200414568716
milk fatty acid composition of cows fed a total mixed ration or pasture plus concentrates replacing corn with fat.thirty-one holstein cows (six ruminally cannulated) were used to evaluate milk fatty acids (fa) composition and conjugated linoleic acid (cla) content on three dietary treatments: 1) total mixed rations (tmr), 2) pasture (avena sativa l.) plus 6.7 kg dm/d of corn-based concentrate (pcorn), and 3) pasture plus pcorn with 0.8 kg dm/d of ca salts of unsaturated fa replacing 1.9 kg dm/d of corn (pfat). no differences were found in total (22.4 kg/d) or pasture (18.5 kg/d) dry matter intake, ruminal p ...200314594244
reduction of crop contamination by soil resuspension within the 30-km zone of the chernobyl nuclear power plant.a field experiment was conducted within the 30-km zone of the chernobyl nuclear power plant to analyze whether the application of mulching reduced resuspension of 137cs contaminated soil in oat (avena sativa) crops. in 1993, we applied a mulch treatment at a dose of 200 g m(-2), and soil resuspension was measured by estimating soil loadings onto plant surfaces from ti concentrations in plants. in 1994, two mulch doses were applied, 200 and 50 g m(-2), and we estimated the contribution of soil re ...200314594366
tissue-specific and subcellular localization of phototropin determined by immuno-blotting.phototropin (phot) is a uv/blue- light receptor mediating phototropic reactions of plants as a response to unilateral irradiation. using an antiserum directed against the n-terminal part of arabidopsis phot1, we show here cross-reaction with phototropin from avena sativa, eruca sativa, glycine max, lepidium sativum, lycopersicon esculentum, pisum sativum, sinapis alba, and zea mays. in all investigated plants, blue light irradiation led to a gel mobility shift of phototropin corresponding to an ...200414634818
[genetic analysis of oat (avena sativa l.) by joint scaling test].comparing the results of genetic analysis of oat varieties with the method of diallel analysis of f1 hybrids and with the joint scaling test the spheres of optimal application of both methods were determined. quantitative estimation of genetic parameters forming the phenotypic averages in scaling test develops the necessary base for involvement of marker genes for qualitative characters in search of qtls controlling the traits with continuous variation. the crosses being the most suitable for fu ...201514650325
oat cover cropping and soil insecticides in an integrated sugarbeet root maggot (diptera: otitidae) management program.sugarbeet, beta vulgaris l., producers occasionally establish cereal cover crops to minimize early-season soil erosion, wind abrasion, and mechanical injury of seedlings. we evaluated the use of living oat, avena sativa l., cover cropping as a cultural tactic to minimize feeding injury from sugarbeet root maggot, tetanops myopaeformis (röder), larvae at five field sites during 1996, 1998, and 1999. sweep-net sampling yielded 4.8-, 11.2-, and 7.2-fold more flies from oat cover-cropped chlorpyrifo ...200314650515
europium uptake and partitioning in oat (avena sativa) roots as studied by laser-induced fluorescence spectroscopy and confocal microscopy profiling technique.the uptake of eu3+ by elongating oat roots was studied by fluorescence spectroscopy, fluorescence lifetime measurement, and a laser excitation time-resolved confocal fluorescence profiling technique. the results of this work indicated that initial uptake of eu3+ was highest within the undifferentiated cells of the root tip just behind the root cap, a region of maximal cell growth and differentiation and with incomplete formation of the casparian strip around the central vascular cylinder. distri ...200314655714
willet m. hays, great benefactor to plant breeding and the founder of our association.willet m. hays was a great benefactor to plant breeding and the founder of the american genetic association (aga). we commemorate the aga's centennial. we mined university archives, u.s. department of agriculture (usda) yearbooks, plant breeding textbooks, scientific periodicals, and descendants for information. willet hays first recognized the individual plant as the unit of selection and started systematic pure-line selection and progeny tests in 1888. he developed useful plant breeding method ...200314691309
joint effects of elevated levels of ultraviolet-b radiation, carbon dioxide and ozone on plants.there is growing interest regarding the joint effects of elevated levels of surface ultraviolet b (uv-b) radiation, carbon dioxide (co2) and ozone (o3) on plants. our current knowledge of this subject is too limited to draw any specific conclusions, although one might state that such effects are likely to be highly species dependent and may be more than additive, additive or less than additive. there are a number of uncertainties associated with the experimental protocols used and the conclusion ...200314743861
production and characterization of hemicellulase activities from trichoderma harzianum strain t4.xylan and mannan are the major constituent groups of hemicellulose in the cell wall of higher plants. the mesophilic fungus trichoderma harzianum strain t4 produces extracellular xylanase and mannanase activities when grown in the presence of oat (avena sativa)-spelt xylan and wheat bran as the carbon sources respectively. after the growth procedure, the crude extracts were submitted to ultrafiltration in an amicon system fitted with a 10 kda-cut-off membrane. mannanase activity was only detecte ...200414763904
a molecular linkage map with associated qtls from a hulless x covered spring oat population.in spring-type oat ( avena sativa l.), quantitative trait loci (qtls) detected in adapted populations may have the greatest potential for improving germplasm via marker-assisted selection. an f(6) recombinant inbred (ri) population was developed from a cross between two canadian spring oat varieties: 'terra', a hulless line, and 'marion', an elite covered-seeded line. a molecular linkage map was generated using 430 aflp, rflp, rapd, scar, and phenotypic markers scored on 101 ri lines. this map w ...200414767596
maize centromeres: organization and functional adaptation in the genetic background of oat.centromeric dna sequences in multicellular eukaryotes are often highly repetitive and are not unique to a specific centromere or to centromeres at all. thus, it is a major challenge to study the fine structure of individual plant centromeres. we used a dna fiber-fluorescence in situ hybridization approach to study individual maize (zea mays) centromeres using oat (avena sativa)-maize chromosome addition lines. the maize centromere-specific satellite repeat centc in the addition lines allowed us ...200414973167
nitric oxide and reactive oxygen species do not elicit hypersensitive cell death but induce apoptosis in the adjacent cells during the defense response of oat.nitric oxide (no) acts as a signaling molecule in many cellular responses in plants and animals. oat plants (avena sativa l.) evoke the hypersensitive response (hr), which shares morphological and biochemical features with mammalian apoptosis, such as dna laddering and heterochromatin condensation, in response to the avirulent crown rust fungus (puccinia coronata f. sp. avenae). we examined the role of no and reactive oxygen species (ros) in the initiation of hypersensitive cell death, which is ...200415000391
purification and characterization of serine proteases that exhibit caspase-like activity and are associated with programmed cell death in avena sativa.victoria blight of avena sativa (oat) is caused by the fungus cochliobolus victoriae, which is pathogenic because of the production of the toxin victorin. the victorin-induced response in sensitive a. sativa has been characterized as a form of programmed cell death (pcd) and displays morphological and biochemical features similar to apoptosis, including chromatin condensation, dna laddering, cell shrinkage, altered mitochondrial function, and ordered, substrate-specific proteolytic events. victo ...200415020745
enrichment of conjugated linoleic acid in oats (avena sativa l.) by microbial isomerization.a method for microbial isomerization of oat linoleic acid to conjugated linoleic acid (cla) was developed. the method includes hydrolysis of oat lipids in aqueous flour slurries by the endogenous oat lipase. then, the flour slurry containing free linoleic acid is utilized as a substrate for the isomerization reaction carried out by resting cells of propionibacterium freudenreichii ssp. shermanii. the isomerization reaction progressed most effectively when, after the lipid hydrolysis period, the ...200415030240
thiamine binding and metabolism in germinating seeds of selected cereals and legumes.the basic characteristics of thiamine metabolism in germinating seeds of maize (zea mays), oat (avena sativa), faba bean (vicia faba) and garden pea (pisum sativum) are presented with a special emphasis of a possible thiamine storage function of seed thiamine-binding proteins (tbps). seeds were germinated for 6 d in the dark. thiamine-binding activity in seeds decreased during germination by 50% in cereals and by 30% in legumes. the degradation of tbps was also detected by polyacrylamide gel ele ...200415051042
purification and properties of apoplastic amylase from oat (avena sativa) seedlings.the protein fraction extracted with a high ionic strength buffer from the cell wall preparation of oat (avena sativa l.) coleoptiles and first leaves contained starch-degrading (amylase) activity. the activity of apoplastic amylase in the coleoptiles and first leaves continued to increase in parallel with organ growth. one of the apoplastic amylases recovered from shoot cell wall preparations was purified by sequential ion exchange and gel filtration chromatography, and the catalytic properties ...200415086825
exposure of oats, avena sativa l., to filtered and unfiltered air in open-top chambers: effects on grain yield and quality.field grown oats, avena sativa l. cv vital, were exposed to filtered and unfiltered air from anthesis until harvest in open-top chambers at a site in south-west sweden. ambient plots were used to study the influence of the chamber itself. with the exception of the number of grains per panicle, which was significantly higher in the charcoal-filtered treatment, no significant filtration effects were obtained for any of the plant growth parameters studied), i.e. grain yield, number of panicles per ...199415091629
reduction of net photosynthesis in oats after treatment with low concentrations of ozone.oats (avena sativa l. cv titus) were exposed to low concentrations of o3 in an assimilation chamber system. net photosynthesis (net co2 uptake), measured before and after o3 fumigation, showed significantly different responses for leaves of different age. the oldest active leaf was the most sensitive to o3. net photosynthesis was depressed after 2 h with 0.075 ppm (150 microg m(-3)) o3. for leaves exposed to 0.150 ppm (300 microg m(-3)) o3 for 2 h, net photosynthesis was reduced significantly fo ...198815092555
effect of ozone on net photosynthesis in oat (avena sativa) and duckweed (lemna gibba).short exposure to ozone depressed photosynthesis in both oat and duckweed at concentrations above 140 microg m(-3) and 300 microg m(-3), respectively. the effect on exposed oat flag leaves was age-dependent, with maximum susceptibility to ozone 10-20 days after emergence of the panicle. in duckweed, photosynthesis was more sensitive to differences in ozone concentration than to differences in duration of exposure.198715092702
effect of sludge-processing mode, soil texture and soil ph on metal mobility in undisturbed soil columns under accelerated loading.the effect of sludge processing (digested dewatered, pelletized, alkaline-stabilized, composted, and incinerated), soil type and initial soil ph on trace metal mobility was examined using undisturbed soil columns. soils tested were hudson silt loam (glossaquic hapludalf) and arkport fine sandy loam (lamellic hapludalf), at initial ph levels of 5 and 7. sludges were applied during four accelerated cropping cycles (215 tons/ha cumulative application for dewatered sludge; equivalent rates for other ...200015092904
repression of pseudomonas putida phenanthrene-degrading activity by plant root extracts and exudates.the phenanthrene-degrading activity (pda) of pseudomonas putida atcc 17484 was repressed after incubation with plant root extracts of oat (avena sativa), osage orange (maclura pomifera), hybrid willow (salix alba x matsudana), kou (cordia subcordata) and milo (thespesia populnea) and plant root exudates of oat (avena sativa) and hybrid poplar (populus deltoides x nigra dn34). total organic carbon content of root extracts ranged from 103 to 395 mg l(-1). characterization of root extracts identifi ...200415142245
influence of application volume on herbicide efficacy.herbicide doses used by farmers are often lower than registration doses. one of the reasons put forward is that field assays for registration are performed at relatively high application volumes (typically 300 l ha(-1)), whereas farmers use lower volumes (down to 75 l ha(-1) or even lower). the resulting concentration of the active ingredient(s) and the formulants is supposed to enhance efficacy. to test this hypothesis in the case of specific graminicides, we compared the efficacy of clodinafop ...200315149130
ovarian development and ovipositional preference of the western corn rootworm (coleoptera: chrysomelidae) variant in east central illinois.the rotation of maize, zea mays l., and soybean, glycine max (l.) merr., has been the traditional cultural tactic to manage the western corn rootworm, diabrotica virgifera virgifera leconte, in the corn belt. the reduced effectiveness of this rotation as a pest management tool in east central illinois, northern indiana, and southern michigan can be explained by the shift in the ovipositional behavior of the new variant of western corn rootworm. the objective of this study was to evaluate the inf ...200415154460
changes in ion fluxes during phototropic bending of etiolated oat coleoptiles.this work has been conducted to assist theoretical modelling of the different stages of the blue light (bl)-induced phototropic signalling pathway and ion transport activity across plant membranes. ion fluxes (ca(2+), h(+), k(+) and cl(-)) in etiolated oat coleoptiles have been measured continuously before and during unilateral bl exposure.200415155378
dissecting the maize genome by using chromosome addition and radiation hybrid lines.we have developed from crosses of oat (avena sativa l.) and maize (zea mays l.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. the whole chromosome 10 addition is available only in haploid oat background. most of the maize chromosome disomic addition lines have regular transmission; however, chromosome 5 showed diminished paternal transmission, and chromosome 10 is transmitted to offspring only as a short-arm telosome. t ...200415197265
Displaying items 201 - 300 of 1129