Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| spatiotemporal expression patterns of sialoglycoconjugates during nephron morphogenesis and their regional and cell type-specific distribution in adult rat kidney. | the expression of alpha2,6- and alpha2,3-linked sialic acids on n-glycans was studied in embryonic, postnatal, and adult rat kidney. histochemistry and blotting using polyporus squamosus and sambucus nigra lectins for alpha2,6-linked sialic acids and the maackia amurensis lectin for alpha2,3-linked sialic acids were performed and sialyltransferase activity was assayed. n-glycans with alpha2,6- and alpha2,3-linked sialic acid were differently expressed in the two embryonic anlagen and early stage ... | 2003 | 12898273 |
| expression of beta-galactoside alpha2,6 sialyltransferase and of alpha2,6-sialylated glycoconjugates in normal human liver, hepatocarcinoma, and cirrhosis. | beta-galactoside alpha2,6-sialyltransferase (st6gal.i) mediates the addition of alpha2,6-linked sialic acid to glycoproteins. st6gal.i is strongly expressed by the liver and is up-regulated in several cancers, but little is known of its regulation in human liver diseases. we have investigated the expression of st6gal.i and its product, the alpha2,6-sialylated lactosamine, in normal human liver, hepatocarcinoma (hcc), and cirrhosis. we found that both st6gal.i activity and mrna can undergo up- or ... | 2004 | 14514712 |
| the role of m cells of human nasopharyngeal lymphoid tissue in influenza virus sampling. | little is known about the role of the m cells of human nasopharyngeal lymphoid tissue in the sampling of viruses that cause respiratory infections. to clarify whether m cells could function as a gateway for influenza virus into human nasopharyngeal lymphoid tissue, excised adenoid tissue was incubated in media containing influenza a virus for 30, 60, and 90 min, respectively. transmission electron microscopic observation revealed that many influenza viruses adhered to m cell surfaces and were ta ... | 2004 | 14551766 |
| differences between influenza virus receptors on target cells of duck and chicken and receptor specificity of the 1997 h5n1 chicken and human influenza viruses from hong kong. | to study whether influenza virus receptors in chickens differ from those in other species, we compared the binding of lectins and influenza viruses with known receptor specificity to cell membranes and gangliosides from epithelial tissues of ducks, chickens, and african green monkeys. we found that chicken cells contained neu5ac alpha(2-6)gal-terminated receptors recognized by sambucus nigra lectin and by human viruses. this finding explains how some recent h9n2 viruses replicate in chickens des ... | 2003 | 14575133 |
| type i allergy to elderberry (sambucus nigra) is elicited by a 33.2 kda allergen with significant homology to ribosomal inactivating proteins. | patients suffering from allergic rhinoconjunctivitis and dyspnoea during summer may exhibit these symptoms after contact with flowers or dietary products of the elderberry tree sambucus nigra. | 2003 | 14656358 |
| [changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue]. | to investigate the changes of sugar chain structures of alkaline phosphatase (alp) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (hcc). | 2003 | 14697136 |
| differential expression of sialylglycoconjugates and sialidase activity in distinct morphological stages of fonsecaea pedrosoi. | the expression of sialoglycoconjugates in fonsecaea pedrosoi conidia, mycelia, and sclerotic cells was analyzed using influenza a and c virus strains, sialidase treatment, and lectin binding. conidium and mycelium whole cells were recognized by limax flavus (lfa), maackia amurensis (maa), and sambucus nigra (sna) lectins, denoting the presence of surface sialoglycoconjugates containing alpha 2,3- and alpha 2,6-sialylgalactosyl sequences. sialidase-treated conidia reacted more intensively with pe ... | 2004 | 14767636 |
| identification of sialic acids on leishmania donovani amastigotes. | the presence of neu5ac on promastigotes of leishmania donovani, the causative organism of indian visceral leishmaniasis, has been reported recently. here we report the occurrence of neu5ac as a major component on amastigotes, as well as neu5gc, neu5,9ac2 and neu9ac5gc as indicated by fluorimetric high performance liquid chromatography and gas liquid chromatography/electron impact mass spectrometry. furthermore, binding studies with sambucus nigra agglutinin (sna), maackia amurensis agglutinin (m ... | 2004 | 14977047 |
| differential lectin recognition of glycoproteins in choanomastigote-shaped trypanosomatids: taxonomic implications. | the glycoprotein profiles of seven choanomastigote-shaped trypanosomatids (six crithidia spp. and one herpetomonas sp.), which have been suggested to form three distinct taxonomic groups (crithidia, angomonas and strigomonas), were analyzed by western blotting using the lectins limax flavus (lfa), sambucus nigra (sna) and maackia amurensis (maa), which specifically recognize sialic acid residues, and concanavalin a (con a) that recognizes mannose-like residues in glycoconjugates. all lectins sho ... | 2004 | 14987761 |
| carbohydrate structure and differential binding of prostate specific antigen to maackia amurensis lectin between prostate cancer and benign prostate hypertrophy. | serum prostate-specific antigen (psa) assay is widely used for detection of prostate cancer. because psa is also synthesized from normal prostate, false positive diagnosis cannot be avoided by the conventional serum psa test. to apply the cancer-associated carbohydrate alteration to the improvement of psa assay, we first elucidated the structures of psa purified from human seminal fluid. the predominant core structure of n-glycans of seminal fluid psa was a complex type biantennary oligosacchari ... | 2004 | 15044396 |
| analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins. | although the type-2 ribosome-inactivating proteins (sna-i, sna-v, snlrp) from elderberry (sambucus nigra l.) are all devoid of rrna n-glycosylase activity towards plant ribosomes, some of them clearly show polynucleotide-adenosine glycosylase activity towards tobacco mosaic virus rna. this particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. transgenic tobacco (nicotiana tabacum l. cv samsun ... | 2004 | 15066176 |
| the in-vitro response of pollen germination and tube length to different types of acidity. | pollen germination and tube growth are among the most sensitive responses to atmospheric pollution. both these are inhibited by the acidity of the growth medium. pollen grains from two species (pinus cembra l. and sambucus nigra l.) were germinated in media over a range of phs (5.0, 4.5, 4.0, 3.5, 3.0 and 2.5) and six types of acidity (h(2)so(4), hno(3), h(2)so(4): hno(3) in 1:1, 2:1, 3:1 and 5:1 ratio). pollen of the gymnosperm is shown to be more resistant to acidity in the medium. sulphuric a ... | 1990 | 15092214 |
| decreased sialylation of the acute phase protein alpha1-acid glycoprotein in feline infectious peritonitis (fip). | feline infectious peritonitis (fip) is an immune-mediated disease of domestic and exotic felides infected with feline coronavirus. fip is characterized by the overexpression of an acute phase protein, the alpha1-acid glycoprotein (agp). in humans, agp is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. we studied the changes in agp glycosylation in the course of fip. specifically, we focussed our attention ... | 2004 | 15135988 |
| placental glycosylation in peccary species and its relation to that of swine and dromedary. | comparison has been made between glycans at the fetomaternal interface of two tayassu species (new world peccaries or wild pigs) and those of swine (true pigs) and dromedary, which have similar epitheliochorial placentae. plastic sections of near-term fetomaternal interface from tayassu tajacu (120 days gestation) and tayassu pecari (140 days gestation) were stained with 20 lectins and compared with those of swine (109 days) and dromedary (375 days). both tayassu species showed similar staining ... | 2004 | 15193872 |
| nontypeable haemophilus influenzae strain 2019 produces a biofilm containing n-acetylneuraminic acid that may mimic sialylated o-linked glycans. | previous studies suggested that nontypeable haemophilus influenzae (nthi) can form biofilms during human and chinchilla middle ear infections. microscopic analysis of a 5-day biofilm of nthi strain 2019 grown in a continuous-flow chamber revealed that the biofilm had a diffuse matrix interlaced with multiple water channels. our studies showed that biofilm production was significantly decreased when a chemically defined medium lacking n-acetylneuraminic acid (sialic acid) was used. based on these ... | 2004 | 15213170 |
| beta1-integrins in the primary cilium of mdck cells potentiate fibronectin-induced ca2+ signaling. | because beta(1)-integrin is involved in sensing of fluid flow rate in endothelial cells, a function that in madin-darby canine kidney (mdck) cells is confined to the primary cilium, we hypothesized beta(1)-integrin to be an important part of the primary ciliary mechanosensory apparatus in mdck cells. we observed that beta(1)-integrin, alpha(3)-integrin, and perhaps alpha(5)-integrin were localized to the primary cilium of mdck cells by combining lectin and immunofluorescence confocal microscopy. ... | 2004 | 15226154 |
| trypanosome trans-sialidase targets trka tyrosine kinase receptor and induces receptor internalization and activation. | trypanosome trans-sialidase (ts) is a sialic acid-transferring enzyme that hydrolyzes alpha2,3-linked sialic acids and transfers them to acceptor molecules. here we show that a highly purified recombinant ts derived from t. cruzi parasites targets trka receptors on trka-expressing pc12 cells and colocalizes with trka internalization and phosphorylation (ptrka). maackia amurensis lectin ii (mal-ii) and sambucus nigra lectin (sna) block ts binding to trka-pc12 cells in a dose-dependent manner with ... | 2004 | 15240558 |
| stress causes tissue-specific changes in the sialyltransferase activity. | numerous pathological conditions are associated with specific changes in glycosylation. recent studies clearly demonstrated a link between stress and the development and course of many diseases. biochemical mechanisms that link stress and diseases are still not fully understood, but there are some indications that changes in glycosylation are involved in this process. influence of acute and chronic psychological stress on protein sialylation as well as the activity of sialyltransferases, enzymes ... | 2004 | 15241940 |
| glycopeptide derived from hen egg ovomucin has the ability to bind enterohemorrhagic escherichia coli o157:h7. | ovomucin glycopeptide (ogp) was prepared by size exclusion chromatography after pronase digestion of hen egg ovomucin, and the binding of ogp to foodborne pathogens (bacillus cereus,clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella enteritidis, salmonella typhimurium, and staphylococcus aureus) was investigaed. binding assays with biotinylated bacteria as probes in microtiter plates showed that ogp bound to only e. coli o157:h7 among these foodborne pathogens. ... | 2004 | 15373418 |
| [the content of zinc and cadmium in medicinal plants and their infusions]. | the content of cadmium and zinc in the elder (sambucus nigra) and the lime tree (tilia spec.) collected from four different localities in the czech republic was studied. from the elder, the flowers (sambuci flos), pollen, and fruits (sambuci fructus) were collected, from the lime tree, the flowers (tiliae flos) were used. the highest content of zn and cd was found in the pollen of the elder ( zn 65.0-94.4 mg.kg(-1), cd 14.1-43.1 microg.kg(-1)), the lowest in its fruits (zn 4.5-14.7 mg.kg(-1), cd ... | 2004 | 15631004 |
| traditional phytotherapy in central italy (marche, abruzzo, and latium). | in this study, the more significant results of extensive ethnopharmacobotanical research carried out by the author in the years 1977-2000 in 175 localities of three regions of central italy (marche, abruzzo, and latium) have been reported and compared. the usages of 80 species belonging to 36 families are described, of which 71 were used in human therapy and 29 in veterinary medicine. uses are suited with the number of localities in which they have been mentioned. among the wild plant mainly sti ... | 2005 | 15664457 |
| epitope recognition of antibodies that define the sialomucin, endolyn (cd164), a negative regulator of haematopoiesis. | endolyn (cd164) is a sialomucin that functions as an adhesion molecule and a negative regulator of cd34+ cd38- human haematopoietic precursor cell proliferation. the 105a5 and 103b2/9e10 cd164 monoclonal antibodies (mabs), which act as surrogate ligands, recognize distinct glycosylation-dependent classes i and ii epitopes located on domain i of the native and recombinant cd164 proteins. here, we document five new cd164 mabs, the 96 series, that rely on conformational integrity, but not glycosyla ... | 2005 | 15730515 |
| influence of sambucus nigra bark lectin on cell dna under different in vitro conditions. | the biological activity of sambucus nigra bark lectin on chinese hamster cells in vitro was investigated by comet-assay and cytotoxicity testing. mitogenic properties at the concentrations 0.063-0.25 microg/ml (but not higher) were found, and the induction of dna breaks at concentrations 0.5 microg/ml and higher is demonstrated. s. nigra bark lectin at mitogenic concentrations decreased the level of nickel-induced dna damage. the character and mechanism of this lectin protective activity was pro ... | 2005 | 15763496 |
| antiviral activity in vitro of urtica dioica l., parietaria diffusa m. et k. and sambucus nigra l. | parietaria diffusa m. et k., urtica dioica l. (urticaceae) and sambucus nigra l. (caprifoliaceae) are plants usually used in popular medicine of central italy for treating numerous diseases, first of all herpes zoster. several plant products have been described as potential antiviral agents, with special attention being devoted to those having retroviruses as etiological agents, including acquired immunodeficiency syndrome (aids), in which a retrovirus, the designated human immunodeficiency viru ... | 2005 | 15814267 |
| mammalian-like nonsialyl complex-type n-glycosylation of equine gonadotropins in mimic insect cells. | recombinant equine luteinizing hormone/chorionic gonadotropin (elh/cg) was expressed in mimic insect cells, that are commercial stably transformed spodoptera frugiperda (sf9) cells expressing five mammalian genes encoding glycosyltransferases involved in the synthesis of complex-type monosialylated n-glycans. we previously showed that it exhibited no in vivo bioactivity although expressing full in vitro bioactivity, and it was suspected that this was because of insufficient sialylation of elh/cg ... | 2005 | 15814822 |
| phenological observations on shrubs to predict weed emergence in turf. | phenology is the study of periodic biological events. if we can find easily recognizable events in common plants that precede or coincide with weed emergences, these plants could be used as indicators. weed seedlings are usually difficult to detect in turf, so the use of phenological indicators may provide an alternative approach to predict the time when a weed appears and consequently guide management decisions. a study was undertaken to determine whether the phenological phases of some plants ... | 2005 | 15846520 |
| increased sialylation and defucosylation of plasma proteins are early events in the acute phase response. | within hours of turpentine injection to stimulate the acute phase (ap) response in rats, the n-acetylneuraminic acid content of plasma proteins increases and that of fucose decreases, each by about 60%. the two changes are inversely related (r = -0.97). the neuac/gal ratio increases from the normal 0.75 to 1.0 on day 2 of the ap. whereas 50% of the isolated oligosaccharides of normal plasma proteins are retarded on immobilized ricinus communis agglutinin, those from day 2 ap plasma fail to do so ... | 2005 | 15858075 |
| use of multidimensional lectin affinity chromatography in differential glycoproteomics. | this paper reports studies comparing the relative degree of sialylation among human serum glycoproteins carrying complex biantennary n-linked, hybrid, and high-mannose oligosaccharides. comparisons were made by coupling lectin affinity selection with stable isotope coding of peptides from tryptic digests of serum. after proteolysis, samples were split and differentially acetylated with stable isotope coding agents according to either origin or the separation method by which they would be fractio ... | 2005 | 15859596 |
| glycan moiety modifications of feline alpha1-acid glycoprotein in retrovirus (fiv, felv) affected cats. | alpha1-acid glycoprotein (agp) is considered one of the major acute phase proteins in cats. in humans, agp is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. in this paper we present the feline agps (fagp) glycan moiety modifications in the course of two prevalent feline diseases, the fiv (feline immunodeficiency virus) dependent feline acquired viral immunodeficiency and the feline leukemia virus (felv) ... | 2005 | 15982476 |
| combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides. | silica-based lectin microcolumns are described in this study together with the chemical procedures necessary for their preparation. the analytical merits of canavalia ensiformis and sambucus nigra lectins, [immobilized on activated macroporous silica], such as binding capacity, trapping reproducibility, and substrate selectivity, have been evaluated using model glycoproteins. the described microcolumns are applicable to high-pressure analytical schemes utilizing microvalving procedures, washing ... | 2005 | 15987113 |
| the blood-brain barrier transmigrating single domain antibody: mechanisms of transport and antigenic epitopes in human brain endothelial cells. | antibodies against receptors that undergo transcytosis across the blood-brain barrier (bbb) have been used as vectors to target drugs or therapeutic peptides into the brain. we have recently discovered a novel single domain antibody, fc5, which transmigrates across human cerebral endothelial cells in vitro and the bbb in vivo. the purpose of this study was to characterize mechanisms of fc5 endocytosis and transcytosis across the bbb and its putative receptor on human brain endothelial cells. the ... | 2005 | 16271053 |
| comparative glycoproteomics of n-linked complex-type glycoforms containing sialic acid in human serum. | this study describes a simple and efficient approach for comparative analysis of sialylated glycoforms of proteins containing differentially branched complex-type glycans. the analytical protocol is based on glycopeptide selection from tryptic digests with serial lectin affinity chromatography (slac), quantification with global internal standard technology, fractionation of deglycosylated peptides with reversed-phase chromatography, and peptide sequencing with tandem mass spectrometry. fractiona ... | 2005 | 16285669 |
| identification of 9-o-acetyl-n-acetylneuraminic acid in normal canine pre-ocular tear film secreted mucins and its depletion in keratoconjunctivitis sicca. | o-acetylated sialic acids have been reported in many sialoglycoproteins where they mediate a variety of immune and other biological events. we have previously demonstrated that the protective mucus barrier on the surface of the canine eye contains sialoglycoproteins. we have also investigated the occurrence of o-acetylated sialic acids in these ocular mucins. mucus aspirated from the surface of normal dog eyes and those with keratoconjunctivitis sicca (kcs) was fractionated into three pools by d ... | 2005 | 16311885 |
| alteration of the sialylation pattern of the murine tooth germ after ethanol exposure. | ethanol consumption during pregnancy leads to changes in murine dental morphogenesis, dental size, cellular differentiation, enamel mineralization, and delayed eruption. it has been proposed that glycoproteins play a role during embryonic dental development that may determine the dental morphological pattern and extracellular matrix secretion. o-glycosylation and sialylation appear to actively participate in the differentiation and maturation processes. because glycosylation may be affected by t ... | 2005 | 16323169 |
| alterations of n-glycan branching and expression of sialic acid on amniotic fluid alpha-1-acid glycoprotein derived from second and third trimesters of normal and prolonged pregnancies. | alterations in the agp glycoform pattern are not only disease related, but also can occur during physiological processes such as pregnancy. in this paper, possible changes in human amniotic fluid agp glycan branching and in the type of sialic acid glycosidic attachment to glycans were analyzed with regard to different stages of human pregnancy. | 2006 | 16405881 |
| inhibition of proinflammatory activities of major periodontal pathogens by aqueous extracts from elder flower (sambucus nigra). | prolonged induction of excessive levels of inflammatory mediators contributes to the pathogenesis of chronic disease states, such as periodontitis. it is thus important to develop safe and effective anti-inflammatory strategies for therapeutic reasons. in this study, we determined the ability of aqueous extracts from elder flower (sambucus nigra) to inhibit the proinflammatory activity of major virulence factors from the periodontal pathogens porphyromonas gingivalis and actinobacillus actinomyc ... | 2006 | 16460254 |
| the use of common elder sambucus nigra to promote aphidophagous syrphids in apple orchards. | elder shrubs (sambucus nigra l.) were planted in an experimental apple orchard as bordering hedgerow with the objective of rearing syrphids (diptera: syrphidae) and consequently enhancing the biological control of the rosy apple aphid dysaphis plantaginea (passerini). the inoculative introduction of the specific elder aphid aphis sambuci l. (homoptera: aphididae) in late march 2002 and again in 2003 led to the complete hedgerow turning into an early and productive alternative prey reservoir for ... | 2005 | 16628887 |
| neoplastic transformation of the thyroid gland is accompanied by changes in cellular sialylation. | cancer of the thyroid gland is one of the most common endocrine diseases. histological evaluation is often complicated by difficulty in distinguishing between benign and malignant lesions. abnormal glycosylation of cell structures, including changes in sialylation, is a feature of the neoplastic transformation process. the aim of this study was to evaluate associations between neoplastic changes in the thyroid gland and changes in sialylation, with reference to its terminal linkage type. lectin ... | 2006 | 16720036 |
| characterisation of insulin-like growth factor receptors and insulin receptors in the human placenta using lectin affinity methods. | insulin and insulin-like growth factor receptors (ir, igf-ir, igf-iir) from human placental cell membranes were solubilised and their glycoprotein properties were studied in terms of their interaction with five lectins: wheat germ agglutinin (wga), banana lectin (banlec), phytohaemagglutinin (pha), concanavalin a (con a), and sambucus nigra agglutinin (sna). the pattern of binding to the immobilised lectins indicated that the glycosylation of the igf-ir, igf-iir and ir differed. we found several ... | 2006 | 16730207 |
| the adhesion of pseudomonas aeruginosa to high molecular weight human tear film species corresponds to glycoproteins reactive with sambucus nigra lectin. | pseudomonas aeruginosa is a pathogen gaining prevalence in contact lens-related corneal ulcers. tear outflow protects the ocular surface, where high molecular weight tear glycoproteins bind bacteria for removal from the eye. the purpose of the present study was to identify glycoproteins in human tears involved in the adhesion of ocular p. aeruginosa isolates. basal human tears were applied to a bacterial adhesion assay involving electrophoretic separation of tear components, transfer to nitrocel ... | 2006 | 16844114 |
| altered granulopoietic profile and exaggerated acute neutrophilic inflammation in mice with targeted deficiency in the sialyltransferase st6gal i. | elevation of serum sialic acid and the st6gal-1 sialyltransferase is part of the hepatic system inflammatory response, but the contribution of st6gal-1 has remained unclear. hepatic st6gal-1 elevation is mediated by p1, 1 of 6 promoters regulating the st6gal1 gene. we report that the p1-ablated mouse, siat1deltap1, and a globally st6gal-1-deficient mouse had significantly increased peritoneal leukocytosis after intraperitoneal challenge with thioglycollate. exaggerated peritonitis was accompanie ... | 2006 | 16849643 |
| [different receptor specificity of influence viruses from ducks and chickens and its reflection in the composition of sialosides on host cells and mucins]. | the ability of influenza viruses from different hosts to bind to the intestinal epithelium of various birds (anseriformes (anatidae), galliformes, charadriiformes (sandpipers and sea gulls), ciconiiformes (storks), podicipediformes (grebes), and gruiformes was studied. the composition of sialo-containing receptors on the epithelia was examined, by using lectins. intestinal epitheliocytes of the anatidae (anseriformes) family was shown to have a low content of receptors binding both sambucus nigr ... | 2006 | 16929595 |
| lower expression of the alpha2,3-sialylated fibronectin glycoform and appearance of the asialo-fibronectin glycoform are associated with high concentrations of fibronectin in human seminal plasma with abnormal semen parameters. | sialic acid isoforms expressed on glycoconjugates are known to be involved in different biological functions. the aim of this study was to compare alpha2,3- and/or alpha2,6-linked sialic acid expression on fibronectin in the seminal plasma of male partners from infertile couples. the data obtained were analyzed in relation to normal and abnormal semen parameters, as well as to fibronectin concentrations. | 2006 | 16958607 |
| beta-galactoside alpha2,6-sialyltransferase and the sialyl alpha2,6-galactosyl-linkage in tissues and cell lines. | beta-galactoside alpha2,6-sialyltransferase (st6gal.i) is the principal sialyltransferase responsible for the biosynthesis of the sialyl alpha2,6-galactosyl linkage. this enzyme and its cognate glycosidic structure are overexpressed in several malignancies and are related to cancer progression. the expression of the enzyme is regulated primarily through the expression of three principal mrna species differing in the 5'-untranslated exons. the form known as yz is considered associated with the ba ... | 2006 | 17072010 |
| an ethnobotanical study on the usage of wild medicinal herbs from kopaonik mountain (central serbia). | an ethnobotanical survey was carried out on the territory of the highest mountain in central serbia, kopaonik, which is characterized by great plant diversity. in total, 83 wild species from 41 families and 96 preparations for use in human therapy were recorded. among those wild plants which are most commonly used for medicinal purposes, hypericum perforatum l., urtica dioica l., achillea millefolium l., matricaria chamomilla l., sambucus nigra l., and thymus serpyllum l. were particularly highl ... | 2007 | 17145148 |
| alpha2,6-linked sialic acid acts as a receptor for feline calicivirus. | feline calicivirus (fcv) is a major causative agent of respiratory disease in cats. it is also one of the few cultivatable members of the family caliciviridae. it has recently been reported that fcv binding is in part due to interaction with junction adhesion molecule-a. this report describes the characterization of additional receptor components for fcv. chemical treatment of cells with sodium periodate showed that fcv recognized carbohydrate moieties on the surface of permissive cells. enzymic ... | 2007 | 17170450 |
| angiotensinogen impairs angiogenesis in the chick chorioallantoic membrane. | angiotensinogen shares with other members of the serine protease inhibitor (serpin) family antiangiogenic properties. angiotensinogen inhibits in vitro endothelial cell proliferation, and is antiangiogenic in ovo in the chick chorioallantoic membrane assay. the cellular mode of action of angiotensinogen has been studied by applying purified human angiotensinogen or chinese hamster ovary cells producing recombinant angiotensinogen onto the developing chorioallantoic membrane. vessel density of th ... | 2007 | 17180343 |
| the influence of laparoscopic and open surgery on the concentration and structural modifications of insulin-like growth factor binding protein 3 in the human circulation. | insulin-like growth factor binding protein 3 (igfbp-3) is the most abundant insulin-like growth factor binding protein in the circulation. the aim of the present work was to investigate the influence of surgery (laparoscopy and open) on the concentration and carbohydrate content of igfbp-3. the concentration of igfbp-3 was measured using an immunoradiometric assay (irma), its protein profile was characterised using immunoblotting and its sialic acid content was examined by means of sambucus nigr ... | 2006 | 17191668 |
| differential expression of glycans in the hippocampus of rats trained on an inhibitory learning paradigm. | the glycan chains of glycoconjugates play important roles in cell-cell and cell-matrix interactions. in the cns, previous studies on learning and memory suggest the importance of oligosaccharides attached to glycoconjugates in the modulation of synaptic connections. we studied the hippocampal glycan distribution of rats subject to an inhibitory avoidance task. the expression of glycans was examined by lectin-histochemistry using vicia villosa lectin (vvl) for terminal alpha/beta n-acetylgalactos ... | 2006 | 17203585 |
| amino acid change 335 e to k affects the sialic-acid-binding and neuraminidase activities of urabe am9 mumps virus hemagglutinin-neuraminidase glycoprotein. | a mutation coding for the amino acid change e335 to k is frequently found in the hemagglutinin-neuraminidase (hn) gene of urabe am9 mumps viruses isolated during post-vaccination meningitis cases. to identify if this mutation modifies the biological activities of the hn glycoprotein, two variants of urabe am9 vaccine differing at amino acid 335 (hn-e335 and hn-k335) were isolated and their receptor-binding specificity was determined by means of competence assays. pre-incubation of the viruses wi ... | 2007 | 17223599 |
| n-glycans of recombinant human acid alpha-glucosidase expressed in the milk of transgenic rabbits. | pompe disease is a lysosomal glycogen storage disorder characterized by acid alpha-glucosidase (gaa) deficiency. more than 110 different pathogenic mutations in the gene encoding gaa have been observed. patients with this disease are being treated by intravenous injection of recombinant forms of the enzyme. focusing on recombinant approaches to produce the enzyme means that specific attention has to be paid to the generated glycosylation patterns. here, human gaa was expressed in the mammary gla ... | 2007 | 17293352 |
| occurrence of n-phenylpropenoyl-l-amino acid amides in different herbal drugs and their influence on human keratinocytes, on human liver cells and on adhesion of helicobacter pylori to the human stomach. | thirty commonly used medicinal plants were screened by a selective and specific lc-ms/ms method for the occurrence of n-phenylpropenoyl- l-amino acid amides, a new homologous class of secondary products. in 15 plants, one or more of the respective derivatives (1 to 12) were found and quantitated. especially roots from angelica archangelica, fruits of cassia angustifolia, c. senna, coriandrum sativum, leaves from hedera helix, flowers from lavandula spec. and from sambucus nigra contained high am ... | 2007 | 17295182 |
| the quail and chicken intestine have sialyl-galactose sugar chains responsible for the binding of influenza a viruses to human type receptors. | the receptor specificity of influenza viruses is one factor that allows avian influenza viruses to cross the species barrier. the recent transmissions of avian h5n1 and h9n2 influenza viruses from chickens and/or quails to humans indicate that avian influenza viruses can directly infect humans without an intermediate host, such as pigs. in this study, we used two strains of influenza a virus (a/pr/8/34, which preferentially binds to an avian-type receptor, and a/memphis/1/71, which preferentiall ... | 2007 | 17389652 |
| colds and influenza: a review of diagnosis and conventional, botanical, and nutritional considerations. | the common cold is the leading cause of doctor visits in the united states and annually results in 189 million lost school days. in the course of one year the u.s. population contracts approximately 1 billion colds. influenza infection is still a leading cause of morbidity and mortality, accounting for 20-25 million doctor visits and 36,000 deaths per year in the united states. conventional therapies for colds and flu focus primarily on temporary symptom relief and include over-the-counter antip ... | 2007 | 17397266 |
| lectin binding profile of the small intestine of five-week-old pigs in response to the use of chlortetracycline as a growth promotant and under gnotobiotic conditions. | antibiotics have traditionally been used for growth promotion in the pork industry; however, their use in animal feed has recently been limited because of human health concerns. the intestinal microbiota plays an important role in mediating many physiological functions such as digestion and animal growth. it was hypothesized that use of antibiotics as growth promotants and subsequent variations in intestinal microbiota induce significant changes in the intestinal glycoconjugate composition, whic ... | 2007 | 17400973 |
| wild vascular plants gathered for consumption in the polish countryside: a review. | this paper is an ethnobotanical review of wild edible plants gathered for consumption from the end of the 18th century to the present day, within the present borders of poland. | 2007 | 17433114 |
| comparison of 226ra nuclide from soil by three woody species betula pendula, sambucus nigra and alnus glutinosa during the vegetation period. | the uptake of 226ra from the contaminated soil was compared in three woody species: alder (alnus glutinosa), birch (betula pendula) and elder (sambucus nigra). the 226ra activities increased during the vegetation periods (in 2003, 2004 and 2005) both in the leaves and flowers+seeds. the highest accumulation was found in birch, reaching 0.41 bq/g dw in the leaves (at the end of the vegetation period in 2003). the lowest 226ra accumulation was determined in alder. the extent of 226ra accumulation ... | 2007 | 17467859 |
| the importance of a taste. a comparative study on wild food plant consumption in twenty-one local communities in italy. | a comparative food ethnobotanical study was carried out in twenty-one local communities in italy, fourteen of which were located in northern italy, one in central italy, one in sardinia, and four in southern italy. 549 informants were asked to name and describe food uses of wild botanicals they currently gather and consume. data showed that gathering, processing and consuming wild food plants are still important activities in all the selected areas. a few botanicals were quoted and cited in mult ... | 2007 | 17480214 |
| first comprehensive contribution to medical ethnobotany of western pyrenees. | an ethnobotanical and medical study was carried out in the navarre pyrenees, an area known both for its high biological diversity and its cultural significance. as well as the compilation of an ethnopharmacological catalogue, a quantitative ethnobotanical comparison has been carried out in relation to the outcomes from other studies about the pyrenees. a review of all drugs used in the area has also been carried out, through a study of the monographs published by the institutions and organizatio ... | 2007 | 17553138 |
| age effect on human aortic valvular glycoproteins. | the aortic valve has been the subject of many hemodynamic studies but, to our knowledge, posttranslational modification of human valve proteins has not yet been studied. thus, the aim of this study was to determine whether any age-related changes in the protein composition of normal human aortic valves and their glycosylation pattern could be observed. | 2007 | 17560454 |
| herbal medicines as diuretics: a review of the scientific evidence. | there is increasing interest in the health and wellness benefits of herbs and botanicals. this is with good reason as they might offer a natural safeguard against the development of certain conditions and be a putative treatment for some diseases. one such area may be the lowering of blood pressure in those where it is elevated (i.e., hypertension). one class of clinical medicines used to lower blood pressure are known as diuretics and work by increasing the excretion of urine from the body as w ... | 2007 | 17804183 |
| identification of n-glycosylated proteins from the central nervous system of drosophila melanogaster. | although the function of many glycoproteins in the nervous system of fruit flies is well understood, information about the glycosylation profile and glycan attachment sites for such proteins is scarce. in order to fill this gap and to facilitate the analysis of n-linked glycosylation in the nervous system, we have performed an extensive survey of membrane-associated glycoproteins and their n-glycosylation sites isolated from the adult drosophila brain. following subcellular fractionation and try ... | 2007 | 17893096 |
| avian influenza receptor expression in h5n1-infected and noninfected human tissues. | avian and human influenza viruses preferentially bind to alpha-2,3-linked and alpha-2,6-linked sialic acids, respectively. until today, the distributions of these two receptor types had never been investigated in h5n1-infected human tissue samples. here, the expression of avian (aiv-rs) and human influenza receptors (huiv-rs) is studied in various organs (upper and lower respiratory tracts, brain, placenta, liver, kidney, heart, intestines, and spleen) of two h5n1 cases and 14 control cases. his ... | 2008 | 17925493 |
| characterization of glycans in the developmental stages of myxobolus cerebralis (myxozoa), the causative agent of whirling disease. | glycans and sugar-binding molecules (lectins) form an interactive recognition system, which may enable parasitic organisms to adhere to host cells and migrate into target tissues. the aim of the present study was to analyse surface-associated glycans in the developmental stages of myxobolus cerebralis (hofer), the causative agent of whirling disease. a panel of biotin-labelled plant lectins was used to detect a broad spectrum of glycan motifs with high specificity. binding sites were detected hi ... | 2007 | 17958607 |
| sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses. | influenza virus binds to cell receptors via sialic acid (sa) linked glycoproteins. they recognize sa on host cells through their haemagglutinins (h). the distribution of sa on cell surfaces is one determinant of host tropism and understanding its expression on human cells and tissues is important for understanding influenza pathogenesis. the objective of this study therefore was to optimize the detection of alpha2,3-linked and alpha2,6-linked sa by lectin histochemistry by investigating the bind ... | 2007 | 17961210 |
| complementary medicine for treating or preventing influenza or influenza-like illness. | the objective of this review was to assess the evidence for the effectiveness of complementary and alternative therapies for preventing or treating influenza or influenza-like illness, including avian influenza. systematic literature searches were conducted in 5 databases until june 2006; other data sources included bibliographies of located articles, manufacturers of commercially available preparations, and experts in the field. randomized clinical trials, controlled against placebo or active c ... | 2007 | 17976414 |
| cell surface glycosylation diversity of embryonic thymic tissues. | in the thymus, glycosylation status of many cell surface molecules changes during the thymocyte maturation and selection processes. in this study, we evaluated the glycosylation changes and possible relationships with programmed cell death in the thymic tissues from mouse embryos at the days 14 (e14), 15 (e15), 16 (e16), 17 (e17) and 18 (e18) of embryonic development. in order to determine glycosylation changes we used three different plant lectins: peanut agglutinin (pna), maackia amurensis leu ... | 2008 | 18028987 |
| placental expression of alpha2,6-linked sialic acid is upregulated in malaria. | in africa, approximately 25 million pregnant women are at risk of plasmodium falciparum infection each year, one in four has evidence of placental involvement and up to half of these may be associated with low birth weight outcomes. in infected pregnant women, the placenta is an ideal site for the accumulation of the parasites, and this reduces in extent in subsequent pregnancies. recent data indicate that terminal alpha2,3 sialic acid-dependent routes are central to the efficient invasion of er ... | 2008 | 18243302 |
| [effect of lectins on induction of apoptosis in the populations of mammalian cells in vitro]. | the cytotoxic action of lectins different in origin and carboxyl specificity has been studied. it has been shown that all types of lectins at high concentrations (20 mkg/ml) were able to induce apoptosis in the in vitro populations of chinese hamster cells two days after the treatment. in the case of persa fluviatilis lectin this effect was detected immediately after the treatment and two days later as well. it was shown that sambucus nigra lectin did not influence the frequency of apoptosis in ... | 2007 | 18268967 |
| detection of carbohydrate terminals in the enteric parasite enteromyxum scophthalmi (myxozoa) and possible interactions with its fish host psetta maxima. | the existence and localisation of carbohydrate terminals in enteromyxum scophthalmi stages was investigated at light (lm) and transmission electron microscopes (tem) using lectin histochemistry techniques, with the aim of contributing to elucidate the participation of carbohydrate-lectin interactions in the parasite invasion and relationships with the fish host. the presence of abundant mannose and/or glucose residues was demonstrated by the intense staining by concanavalin a at both lm and tem. ... | 2008 | 18311571 |
| an observational study and quantification of the actives in a supplement with sambucus nigra and asparagus officinalis used for weight reduction. | the aim of the study was to obtain information on the content of co-active compounds of a food supplement recommended as a weight reduction diet and on its short-term effectiveness and safety as a starter for lifestyle change. eighty participants completed the protocol. the sambucus nigra l. berry juice enriched with flower extract and tablets containing berry powder and flower extract provided a total of 1 mg anthocyanins, 370 mg flavonol glycosides and 150 mg hydroxycinnamates per day; the asp ... | 2008 | 18350516 |
| [lectins from sambucus nigra l inflorescences: isolation and investigation of biological activity using procaryotic test-systems]. | isolation of lectins from extracts of the sambucus nigra inflorescences and of pollen material have been performed using isoelectric focusing without carrier ampholytes (autofocusing). fractions active in agglutination tests with different carbohydrate specificity were subjected to sds-page. the major lectin found in whole inflores-cences was galnac specific and is proposed to be a heterotetramer with subunits of about 30 and 33 kda. it was called snaflu-i. at least two other lectins were presen ... | 2007 | 18357788 |
| surface plasmon resonance study of protein-carbohydrate interactions using biotinylated sialosides. | lectins are carbohydrate binding proteins found in plants, animals, and microorganisms. they serve as important models for understanding protein-carbohydrate interactions at the molecular level. we report here the fabrication of a novel sensing interface of biotinylated sialosides to probe lectin-carbohydrate interactions using surface plasmon resonance spectroscopy (spr). the attachment of carbohydrates to the surface using biotin-neutravidin interactions and the implementation of an inert hydr ... | 2008 | 18461973 |
| monitoring of influenza virus hemagglutinin in process samples using weak affinity ligands and surface plasmon resonance. | surface plasmon resonance (spr) was used to screen the interaction between a variety of affinity ligands and hemagglutinin (ha) from human influenza virus, with the aim of identifying low affinity ligands useful for the development of a rapid bioanalytical sensor. three sialic acid-based structures and four lectins were evaluated as sensor ligands. the sialic acid-based ligands included a natural sialic acid-containing glycoprotein, human alpha1-acid glycoprotein (alpha1-agp), and two synthetic ... | 2008 | 18611459 |
| pericytes in the mature chorioallantoic membrane capillary plexus contain desmin and alpha-smooth muscle actin: relevance for non-sprouting angiogenesis. | the chicken chorioallantoic membrane (cam) is a frequently used tissue for studying vascular growth and remodeling, notably non-sprouting angiogenesis by formation of transluminal pillars. vascular pericytes have received increasing attention in the field of angiogenesis research and appear important for pillar growth. our earlier observation that desmin (des), but not alpha-smooth muscle actin (alphasma) was expressed in pericytes of the mature cam capillary plexus after e12 was confirmed by ot ... | 2008 | 18688635 |
| expression of sambucus nigra agglutinin (sna-i') from elderberry bark in transgenic tobacco plants results in enhanced resistance to different insect species. | tobacco plants (nicotiana tabacum cv samsun nn) have been transformed with the gene encoding the type-2 ribosome-inactivating protein (rip) sna-i' from elderberry (sambucus nigra) under the control of the cauliflower mosaic virus 35s promoter. previous research confirmed that these plants synthesize, correctly process and assemble a fully active rip. variability in protein expression was observed within the transgenic lines. the effects of the type-2 rip sna-i' delivered through a leaf feeding a ... | 2009 | 18720022 |
| combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides. | although the vital roles of structures containing sialic acid in biomolecular recognition are well documented, limited information is available on how sialic acid structural modifications, sialyl linkages, and the underlying glycan structures affect the binding or the activity of sialic acid-recognizing proteins and related downstream biological processes. a novel combinatorial chemoenzymatic method has been developed for the highly efficient synthesis of biotinylated sialosides containing diffe ... | 2008 | 18729452 |
| influence of altitudinal variation on the content of phenolic compounds in wild populations of calluna vulgaris, sambucus nigra, and vaccinium myrtillus. | this study deals with the effect of altitudinal variation on the content of phenolic compounds in three traditional herbal plants, which are also consumed as food in central europe. herbs of calluna vulgaris (l.) hull, flowers and fruits of sambucus nigra l., and berries of vaccinium myrtillus l. collected in the naturpark solktaler (austria) were extracted using accelerated solvent extraction (ase). identification and quantification of the constituents in the polar extracts (methanol 80%, v/v) ... | 2008 | 18788745 |
| the role of integrin glycosylation in galectin-8-mediated trabecular meshwork cell adhesion and spreading. | primary open angle glaucoma (poag) is a major blindness-causing disease, characterized by elevated intraocular pressure due to an insufficient outflow of aqueous humor. the trabecular meshwork (tm) lining the aqueous outflow pathway modulates the aqueous outflow facility. tm cell adhesion, cell-matrix interactions, and factors that influence rho signaling in tm cells are thought to play a pivotal role in the regulation of aqueous outflow. in a recent study, we demonstrated that galectin-8 (gal8) ... | 2009 | 18849583 |
| carbohydrate-binding activity of the type-2 ribosome-inactivating protein sna-i from elderberry (sambucus nigra) is a determining factor for its insecticidal activity. | in recent years, different classes of proteins have been reported to promote toxic effects when ingested. type-2 ribosome-inactivating proteins (rips) are a group of chimeric proteins built up of an a-chain with rna n-glycosidase activity and a b-chain with lectin activity. these proteins are thought to play a role in plant protection. sambucus nigra agglutinin i (sna-i) is a type-2 rip, isolated from the bark of elderberry (s. nigra l.). this study demonstrated the insecticidal potency of sna-i ... | 2008 | 18951590 |
| exposure of alpha2,6-sialylated lactosaminic chains marks apoptotic and necrotic death in different cell types. | many observations have reported glycosylation changes associated with apoptosis in different biological systems, although none of these has shown any general significance. in this work, we show that in cell lines from different histological origin, (colon, breast, pancreas, and bladder cancer) as well as in normal human and mice neutrophils, apoptosis is accompanied by the exposure of sugar chains recognized by the lectin from sambucus nigra (sna), specific for sia alpha 2,6gal/galnac structures ... | 2009 | 18988689 |
| differential n-glycosylation of kallikrein 6 derived from ovarian cancer cells or the central nervous system. | ovarian cancer causes more deaths than any other gynecological disorder. perturbed glycosylation is one of the hallmarks of this malignancy. kallikrein 6 (klk6) elevation in serum is a diagnostic and prognostic indicator in ovarian cancer. the majority of ovarian carcinomas express high levels of klk6, which diffuses into the circulation. under physiological conditions, klk6 is expressed highly in the central nervous system and found at high levels in cerebrospinal fluid from where it enters the ... | 2009 | 19088065 |
| specificity analysis of lectins and antibodies using remodeled glycoproteins. | due to their ability to bind specifically to certain carbohydrate sequences, lectins are a frequently used tool in cytology, histology, and glycan analysis but also offer new options for drug targeting and drug delivery systems. for these and other potential applications, it is necessary to be certain as to the carbohydrate structures interacting with the lectin. therefore, we used glycoproteins remodeled with glycosyltransferases and glycosidases for testing specificities of lectins from aleuri ... | 2009 | 19123999 |
| mucin-type o-glycans in tears of normal subjects and patients with non-sjögren's dry eye. | o-linked carbohydrates (o-glycans) contribute to the hydrophilic character of mucins in mucosal tissues. this study was conducted to identify the repertoire of o-glycans in the tear film and the glycosyltransferases associated with their biosynthesis, in normal subjects and patients with non-sjögren's dry eye. | 2009 | 19407012 |
| [expression of galectin 3 and sambucus nigra agglutinin and their clinicopathological significance in benign and malignant lesions of stomach]. | to study the expressive levels of galectin-3(gal-3) and sambucus nigra agglutinin(sna) and their clinicopathological significance in the benign and malignant lesions of stomach. | 2009 | 19434543 |
| the effects of hypo- and hyperthyroidism on nuclear, cytosolic, endoplasmic and mitochondrial fractions of sialoglycoproteins in rabbit hepatocytes. | enhanced sialylation of glycoproteins occurs during hypo- and hyperthyroidism. the role of sialic acid (sa) in cell membranes is well-standing, but its role in intracellular structures is still under analysis. we wanted to evaluate the influence of thyroid hormones on the sialylation ratio of intracellular proteins presented in cytosolic, mitochondrial, endoplasmic and nuclear fractions of rabbit hepatocytes. twenty-one new zealand male rabbits were divided into three groups. hypothyroidism (n = ... | 2009 | 19445840 |
| enhanced expression of alpha1-acid glycoprotein and fucosylation in hepatitis b patients provides an insight into pathogenesis. | altered glycosylation and concentration of alpha1-acid glycoprotein has been known to be related to the pathogenesis of the hepatic diseases. the present study investigated enhanced fucosylation of agp in the sera of chronic hepatitis b (hbv-ch) and hepatitis b cirrhosis (hbv-lc) patients by high performance anion exchange chromatography and by elisa using fucose binding aleuria aurantia lectin. the concentration of agp determined by elisa using monoclonal anti-human agp (mab-agp) showed high le ... | 2009 | 19459043 |
| sialoglycoconjugates in herpetomonas megaseliae: role in the adhesion to insect host epithelial cells. | herpetomonas megaseliae is a monoxenic trypanosomatid isolated from the phorid fly megaselia scalaris. in the present report, the expression of cell surface sialoglycoconjugates in this parasite was analyzed by western blotting, flow cytometry and fluorescence microscopy analyses using lectins that specifically recognize sialic acid residues. a strong reaction was detected when parasites were treated with limax flavus, maackia amurensis and sambucus nigra lectins. analysis of crude protein extra ... | 2009 | 19459976 |
| sialic acid in human testis and changes with aging. | the aim of the present study was to investigate the distribution of the glycoconjugates sialoderivatives in the human testis. orchidectomy specimens from men aged 18-30 years (group 1) and from men aged 70-93 years (group 2) were obtained at autopsy. the study was performed using digoxigenin-labelled lectins, namely maackia amurensis ii lectin (maa), sambucus nigra agglutinin (sna) and arachis hypogaea lectin (pna), in addition to enzymatic and chemical treatments (neuraminidase, koh-neuraminida ... | 2009 | 19486598 |
| synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat alpha2,6-sialyltransferase expressed in bmnpv bacmid-injected silkworm larvae. | sialic acid is a deoxy uronic acid with a skeleton of nine carbons which is mostly found on cell surface in animals. this sialic acid on cell surface performs various biological functions by acting as a receptor for microorganisms, viruses, toxins, and hormones; by masking receptors; and by regulating the immune system. in order to synthesize an artificial sialoglycoprotein, we developed a large-scale production of rat alpha2,6-sialyltransferase (st6gal1). the st6gal1 was expressed in fifth inst ... | 2009 | 19500344 |
| a systematic review of the efficacy and safety of herbal medicines used in the treatment of obesity. | this review focuses on the efficacy and safety of effective herbal medicines in the management of obesity in humans and animals. pubmed, scopus, google scholar, web of science, and iranmedex databases were searched up to december 30, 2008. the search terms were "obesity" and ("herbal medicine" or "plant", "plant medicinal" or "medicine traditional") without narrowing or limiting search elements. all of the human and animal studies on the effects of herbs with the key outcome of change in anthrop ... | 2009 | 19575486 |
| lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus beauveria bassiana. | the entomopathogenic fungus beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. b. bassiana produces a number of distinct cell types that include aerial conidia, submerged conidia, blastospores and haemolymph-derived cells termed in vivo blastos ... | 2009 | 19608611 |
| bovine adenovirus serotype 3 utilizes sialic acid as a cellular receptor for virus entry. | bovine adenovirus serotype 3 (bad3) and porcine adenovirus serotype 3 (pad3) entry into the host cells is independent of coxsackievirus adenovirus receptor and integrins. the role of sialic acid in bad3 and pad3 entry was investigated. removal of sialic acid by neuraminidase, or blocking sialic acid by wheat germ agglutinin lectin significantly inhibited bad3, but not pad3, transduction of madin-darby bovine kidney cells. maackia amurensis agglutinin or sambucus nigra (elder) agglutinin treatmen ... | 2009 | 19646729 |
| detection of expression of influenza virus receptors in tissues of balb/c mice by histochemistry. | infection of host cells with the influenza virus is mediated by specific interactions between the viral hemagglutinin and its cell receptor, oligosaccharides containing sialic acid (sa) residues. avian and human influenza viruses preferentially bind to α-2, 3-linked and α-2, 6-linked sialic acids, respectively. therefore, differential expression of these receptors may be crucial to influenza virus infection. to date, the distribution of these two receptors has never been investigated in the tiss ... | 2009 | 19662506 |
| elderberry flavonoids bind to and prevent h1n1 infection in vitro. | a ionization technique in mass spectrometry called direct analysis in real time mass spectrometry (dart tof-ms) coupled with a direct binding assay was used to identify and characterize anti-viral components of an elderberry fruit (sambucus nigra l.) extract without either derivatization or separation by standard chromatographic techniques. the elderberry extract inhibited human influenza a (h1n1) infection in vitro with an ic(50) value of 252+/-34 microg/ml. the direct binding assay established ... | 2009 | 19682714 |
| a close look at human igg sialylation and subclass distribution after lectin fractionation. | polyspecific human igg preparations are indicated for the treatment of primary immunodeficiency disorders associated with defects in humoral immunity. in addition, intraveneous igg (ivig) is used to treat patients with autoimmune and systemic inflammatory diseases. lectin chromatography on sambucus nigra agglutinin stood at the cradle of the hypothesis that the anti-inflammatory properties depend on sialylation of the n-glycans in the fc region of igg. a detailed analysis of fractions obtained b ... | 2009 | 19688751 |
| abo blood group glycans modulate sialic acid recognition on erythrocytes. | abh(o) blood group polymorphisms are based on well-known intraspecies variations in structures of neutral blood cell surface glycans in humans and other primates. whereas natural antibodies against these glycans can act as barriers to blood transfusion and transplantation, the normal functions of this long-standing evolutionary polymorphism remain largely unknown. although microbial interactions have been suggested as a selective force, direct binding of lethal pathogens to abh antigens has not ... | 2009 | 19704115 |
| a lectin hplc method to enrich selectively-glycosylated peptides from complex biological samples. | glycans are an important class of post-translational modifications. typically found on secreted and extracellular molecules, glycan structures signal the internal status of the cell. glycans on tumor cells tend to have abundant sialic acid and fucose moieties. we propose that these cancer-associated glycan variants be exploited for biomarker development aimed at diagnosing early-stage disease. accordingly, we developed a mass spectrometry-based workflow that incorporates chromatography on affini ... | 2009 | 19798022 |
| characterisation of n-glycans bound to igfbp-3 in sera from healthy adults. | human igfbp-3 contains three potential n-linked glycosylation sites. published data concerning the type and saccharide composition of the n-glycans is scarce. the aim of this study was to characterise n-glycans covalently attached to igfbp-3 from sera of healthy adults (men and women). in order to do that a panel of eight lectins covering broad saccharide specificity was used: agarose-immobilised sna (sambucus nigra agglutinin), con a (lectin from canavalia ensiformis), rca i (ricinus communis a ... | 2010 | 19800385 |
| the glycosylation pattern of secretory granules in binucleate trophoblast cells is highly conserved in ruminants. | the binucleate trophoblast cells (bncs) in the ruminant placenta are a unique feature of this taxon. these cells produce several secretory proteins and transfer these across the fetomaternal barrier into the dam. we used lectin histochemistry with a panel of 24 lectins to characterise the glycosylation pattern of bnc secretory granules in a variety of ruminants. seven species out of three ruminant families were thus investigated: greater malayan chevrotain (tragulidae); fallow deer, red deer, ch ... | 2010 | 19959226 |