Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rapid production of interferon-gamma in uninduced human leukocyte suspensions. | human leukocytes produced interferon-gamma (ifn-gamma) rapidly without induction during incubation at 37 degrees c. the intracellular level peaked at 3 h; the extracellular ifn-gamma reached its maximum at 7 h. leukocytes purified by different methods, and whether from single donors or pooled from numerous donors, yielded similar amounts of ifn. the best yields were approximately 3 iu/10(6) cells. the highest titers were obtained at cell concentrations ranging between 30 and 100 x 10(6)/ml, and ... | 1991 | 1919078 |
| glycoconjugates of the lateral prostate of the guinea pig: a lectin histochemical study. | the present study examined the glycoconjugates of the lateral prostate using a battery of lectins. the results indicated that the secretory epithelium was rich in mannose (man), n-acetylglucosamine (glcnac), galactose (gal), n-acetylgalactosamine (galnac), and complex oligosaccharides. con a (concanavalin a), lca (lens culinaris agglutinin), psa (pisum sativum agglutinin), wga (wheat germ agglutinin), pwm (pokeweed mitogen), rca-i (racinus communis isolectin i), and pha-p (phaseolus vulgaris agg ... | 1991 | 1923963 |
| radionuclide contamination of foods imported into iraq following the chernobyl nuclear reactor accident. | since early 1986, a monitoring program for radionuclides in imported foods has been carried out by the iraqi atomic energy commission. after the chernobyl nuclear reactor accident in the soviet union, the program was expanded; our laboratory was officially designated by the iraqi government to measure radionuclide activity concentrations in foodstuff imported from countries known to be severely contaminated by chernobyl radioactive fallout. gamma-spectrometric analysis was used. food items such ... | 1991 | 1925528 |
| insulin sensitivity and liver insulin receptor structure in ducks from two genera. | insulin sensitivity and liver insulin receptor structure were studied in 5-wk-old ducks from two genera (muscovy and pekin). in the fasting state, both duck types were equally resistant to exogenous insulin compared with chicken. despite the low potency of duck insulin, the number of insulin receptors was lower in muscovy duck and similar in pekin duck and chicken liver membranes. after 125i-insulin cross-linking, the size of the alpha-subunit of the receptors from the three species was 135,000. ... | 1991 | 1928434 |
| glycosylation at the fab portion of myeloma immunoglobulin g and increased fucosylated biantennary sugar chains: structural analysis by high-performance liquid chromatography and antibody-lectin enzyme immunoassay using lens culinaris agglutinin. | an antibody-lectin enzyme immunoassay technique which had been developed for the analysis of sugar chains of alpha-fetoprotein (n. kinoshita et al., clin. chim. acta, 179: 143-152, 1989) was used for analysis of sugar chains of myeloma immunoglobulin g (igg). the igg sugar chains of four of nine patients with myeloma were found to be highly reactive to lens culinaris agglutinin as compared with those of six normal controls and 177 patients without myeloma. this reflected a high l. culinaris aggl ... | 1991 | 1933856 |
| dietary management of persistent diarrhea: comparison of a traditional rice-lentil based diet with soy formula. | recent studies have indicated that enteral diets can play an important role in the treatment of persistent diarrhea. khitchri, a local weaning food in pakistan, is composed of rice and lentils, which have previously been shown to be well tolerated in many children with acute diarrhea. the effectiveness of a khitchri and yogurt (ky) diet, which is inexpensive and widely available in pakistan, was studied. one hundred two weaned boys (6 to 36 months old) with persistent diarrhea were randomly assi ... | 1991 | 1945604 |
| increased hepatic insulin proreceptor-to-receptor ratio in diabetes: a possible processing defect. | hepatic insulin proreceptors and receptors were studied in control and in ketotic diabetic rats 2-4 wk after streptozotocin treatment. solubilized preparations were partially purified by wheat germ agglutinin-agarose (wga) and lentil lectin agarose (lla) chromatography to enrich eluates in insulin receptors and proreceptors, respectively. after phosphorylation with [gamma-32p]atp, an approximately 190-kda glycoprotein was identified in lla eluates as the insulin proreceptor, based on insulin dos ... | 1991 | 1951680 |
| [nutritional quality of the protein of the false lentil (vicia sativa ssp. abovata (ser) gaudin]. | the common vetch (vicia sativa) is a legume that grows spontaneously in the central zone of chile. it is consumed by the rural population, as lentil, and frequently it is utilized in animal feeding. the objective of the present work was to study the biological quality of its protein and protein digestibility, as well as its dietary fiber pattern. the samples studied were provided by the instituto de investigaciones agropecuarias (inia), where in its chemical composition, dietary fiber, biologica ... | 1990 | 1967029 |
| characterization of gamma-glutamyltranspeptidase from human pancreatic cancer. | to elucidate the specific changes of pancreatic gamma-glutamyl-transpeptidase (gamma-gtp) associated with malignant transformation, some properties of gamma-gtp purified from pancreatic cancer were compared with those of gamma-gtps from normal pancreas and other tissues. four of five pancreatic cancer gamma-gtps showed distinctly slower electrophoretic mobility than normal pancreatic enzymes. isoelectric points of pancreatic cancer gamma-gtps varied in each case, but they were all higher than th ... | 1990 | 1967152 |
| [structural characterization of the somatostatin receptors on rat cerebrocortical membranes]. | we characterized structurally the receptors for somatostatin in rat cerebral cortex by affinity labeling with [125i-tyr1] somatostatin. [125i-tyr1] somatostatin was cross-linked to cerebrocortical membranes using photoreactive cross-linker: n-5-azido-2-nitrobenzoyloxy-succinimide. analysis by autoradiography revealed a broad band centered at mr = 72,000 in the presence or absence of dithiothreitol. affinity labeling of and specific [125i-tyr1] somatostatin binding to cerebrocortical membranes we ... | 1990 | 1980894 |
| glycoproteins in the retinal pigment epithelium of normal and dystrophic rats. | the apical membranes of retinal pigmented epithelium (rpe) were isolated from adult, normal (le), and dystrophic (rcs) rats. the proteins of these rpe subfractions were separated through the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the lectin-binding properties of glycoproteins were examined in western blots through the use of lectin-peroxidase conjugates. no differences were detected between rpe membrane proteins from normal and dystrophic rats in silver-stained gels. h ... | 1991 | 1993583 |
| studies on the oligosaccharide heterogeneity of the isoelectric forms of the lower molecular weight acid phosphatase of frog liver. | 1. the lower molecular weight, heterogeneous acid phosphatase (acpase) from the frog liver (rana esculenta) containing acpase i, ii, iii and iv was separated into enzymatically active components by isoelectric focusing in an immobilized ph gradient. 2. the blotted enzyme bands were characterized by their different binding patterns obtained with the lectins concanavalin a, wheat germ agglutinin (wga), lens culinaris hemagglutinin (lch) and peanut agglutinin (pna). 3. in situ neuraminidase treatme ... | 1991 | 2015951 |
| isolation and characterization of a membrane glycoprotein from human brain with sequence similarities to cell adhesion proteins from chicken and mouse. | we previously described the production of monoclonal antibodies against a preparation of membrane glycoproteins from human brain [berglund et al. (1987) j. neurochem. 48, 809-815]. one of the glycoproteins, recognized by monoclonal antibody cf3, was specifically expressed in the brain. we now report the isolation and characterization of this glycoprotein, called glycoprotein 135 (gp135). gp135 was purified by means of lentil lectin affinity chromatography and immunoaffinity chromatography, using ... | 1991 | 2026173 |
| the collagen binding specificity of bone and platelet osteonectin is related to differences in glycosylation. | in this study we report that bone and platelet osteonectin are structurally and functionally heterogeneous in terms of glycosylation and collagen binding capacity. the relative sensitivity of bone and platelet osteonectin to specific glycosidases was used to evaluate potential differences in glycosylation. although native bone and platelet osteonectin are electrophoretically nonidentical, n-glycanase treatment yielded products with the same apparent molecular weight. bone osteonectin was also su ... | 1991 | 2033056 |
| on the multimeric nature of natural human interleukin-6. | natural human interleukin-6 (il-6) characterized under completely denaturing conditions consists of a set of differentially modified phosphoglycoproteins of molecular mass in the range from 23 to 30 kda ("25-kda" o-glycosylated species and "30-kda" o- and n-glycosylated species). the 25-kda o-glycosylated il-6 (which contains only ser- or thr-galnac-gal-neunac and thus should not bind wheat germ or lentil lectins) bound to and was eluted from a wheat germ lectin affinity column by glcnac and fro ... | 1991 | 2033081 |
| dietary plant materials and development of diabetes in the bb rat. | the present study was designed to examine further the impact of individual plant protein sources found in a diabetogenic, cereal-based, rodent laboratory diet, nih-07 [open formula, nonpurified rat and mouse diet (positive control)], on the development of diabetes. diabetes-prone bb rats that were pan-t(ox19+)-lymphopenic were fed a low diabetogenic diet during gestation and lactation. progeny of these rats were fed a normal or autoclaved nih-07 diet, or one of eight other diets based on the ain ... | 1991 | 2033475 |
| histochemical and immunohistochemical studies of diseased human glomeruli. | in order to study the localization of lentil lectin (lch)-binding glycoresidues in glomeruli from patients with a variety of glomerulopathies, and to elucidate the relationship between lch-binding sugars and the components of the extracellular matrix, laminin and type iv collagen, investigations of formalin-fixed, paraffin-embedded kidney tissues digested with trypsin were carried out by the direct and indirect immunofluorescence microscopy techniques. the glomerular basement membrane (gbm) and ... | 1991 | 2038128 |
| production and characterization of a mouse monoclonal antibody specific for lentil lectin. | a murine iga monoclonal antibody (moab) was produced against the widely used glucose/mannose-specific two-chain mitogen from lens culinaris (lentil) belonging to the vicieae tribe of the leguminosae family. the moab designated, 98, f-10, was found to be specific for lentil lectin when tested in dot blotting against 22 different native lectins. the antigenic specificity was also tested against subunits of 13 completely sequenced legume lectins separated by sodium dodecyl sulphate-polyacrylamide g ... | 1991 | 2039607 |
| structure of a new nervous system glycoprotein, nr-cam, and its relationship to subgroups of neural cell adhesion molecules. | we have identified and characterized a new glycoprotein in the chicken nervous system using immunological and molecular biological methods and we have examined its tissue distribution. analysis revealed that this protein is very similar in structure to the chicken neuron-glia cell adhesion molecule, ng-cam, and to mouse l1. cdna clones encompassing the entire coding sequence of this ng-cam related molecule, called nr-cam, have been isolated and sequenced. a glycoprotein containing one major comp ... | 1991 | 2045418 |
| carbohydrate composition of human megakaryocyte membranes in culture: identification using binding of seven lectins. | seven tetramethylrhodamine b isothiocyanate- (tritc) labeled lectins: lens culinaris (lch), ulex europeus-1 (uea-1), lycopersicon esculentum (lea), wheat germ agglutinin (wga), dolichos biflorus (dba), soybean agglutinin (sba) and erythrina cristagalli (eca) were applied on cultured human megakaryocytes (megs) detected by immunofluorescence. all stages of megs (from lymphocyte-like megs to mature megs) and platelets were labeled by lch, lea, uea-1 and wga. eca binds to platelets but only to some ... | 1991 | 2061623 |
| [lysosomes in embryonic cell differentiation]. | lysosomes as a modeling factor of embryonal morphogenesis occupy an important place in the systemic structural and ultrastructural studies on prenatal development of man. human embryos at the age of 6 to 10 weeks of embryonal development, obtained after interruption of normal pregnancy by the method vacuum excochleation according to andreev. lysosomes were investigated in cells of groups, chosen in advance, from organs with various origin and degree of cellular differentiation: neurones of the s ... | 1990 | 2098255 |
| precocious loss of cortical granules during mouse oocyte meiotic maturation and correlation with an egg-induced modification of the zona pellucida. | fertilization results in cortical granule exocytosis, which is thought to be involved in modifications of the zona pellucida that constitute the zona pellucida block to polyspermy. a previous report demonstrated that a decrease in the number of lens culinaris agglutinin-staining granules, which are likely to be cortical granules, occurred during in vivo mouse oocyte maturation with arrest at metaphase ii, as well as the formation of a cortical granule-free domain in the area of the metaphase ii ... | 1990 | 2104813 |
| purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum. | the 53-kda glycoprotein and sarcalumenin (160-kda glycoprotein) were extracted from rabbit skeletal muscle sarcoplasmic reticulum with egta and purified by fractionation on deae-sephadex a-25 and lentil lectin-sepharose 4b. sarcalumenin was shown to bind up to 400 nmol of ca2+/mg of protein at ph 7.5, which is equivalent to binding of approximately 35 mol of ca2+/mol of protein. the apparent dissociation constant was 300 microm in the presence of 20 mm kcl and 600 microm in 150 mm kcl. the 53-kd ... | 1990 | 2112542 |
| heterogeneity of latent transforming growth factor-beta isolated from bone matrix proteins. | transforming growth factor-beta (tgf beta) is a family of 25-kda peptides that appear to be important regulators of cell proliferation, differentiation, and differentiated function in many tissues. tgf beta is present in platelets and serum and is released by cultured cells as several distinct large mol wt complexes of tgf beta with other proteins. these complexes are biologically inactive and are generically called latent tgf beta (l-tgf beta). large quantities of tgf beta are present in bone m ... | 1990 | 2137079 |
| a cerebellar purkinje cell marker p400 protein is an inositol 1,4,5-trisphosphate (insp3) receptor protein. purification and characterization of insp3 receptor complex. | p400 protein is a 250 kd glycoprotein, characteristic of the cerebellum, which is accumulated at the endoplasmic reticulum, at the plasma membrane and at the post-synaptic density of purkinje cells. in this study, we purified inositol 1,4,5-trisphosphate (insp3) receptor from mouse cerebellum and examined the possibility that p400 protein is identical with cerebellar insp3 receptor protein. insp3 receptor was solubilized with triton x-100 from a post-nuclear fraction of ddy mouse cerebellum and ... | 1990 | 2153079 |
| increased in-vivo insulin sensitivity but normal liver insulin receptor kinase activity in dwarf chickens. | the effects of the recessive and sex-linked dw gene on insulin sensitivity and liver insulin receptors were compared in normal (dw-dw) and dwarf (dw-dw) brother or half-brother chickens. at 3.5 weeks of age, following an overnight fast, exogenous insulin (0-6.9 nmol/kg body weight) was slightly but significantly more hypoglycaemic in dwarf chickens. at 4 weeks of age, following an oral glucose load (2 g/kg), glucose tolerance was the same in both genotypes, whereas plasma insulin levels were gre ... | 1990 | 2166126 |
| adsorptive and fluid phase endocytosis by cultured rabbit corneal endothelium. | in addition to maintaining corneal transparency via a pump-leak mechanism, the corneal endothelium plays an active role in the transport of certain proteins to supply nutrients for the stroma and to remove metabolites. in order to investigate transcellular transport mechanisms across the endothelium, we exposed cultured rabbit corneal endothelial cells to tracers that are commonly used to describe various forms of endocytosis. the cells were incubated on their apical surfaces in solutions contai ... | 1990 | 2189689 |
| n-linked oligosaccharides of the murine transferrin receptor from a plasmacytoma cell line. comparison with total cellular n-glycans. | the n-linked oligosaccharides synthesised by the murine plasmacytoma cell line ns-1 have been analysed by lectin affinity chromatography on columns of immobilised concanavalin a (con a), lens culinaris (lentil), ricinus communis agglutinin (rca) and leuko-phytohemagglutinin (l-pha). the majority of complex n-glycans in this transformed cell line were branched structures with only a low level of biantennary complex chains detected. the analysis showed the major complex n-glycan fraction consisted ... | 1990 | 2223825 |
| clear cell carcinoma of the human ovary synthesizes and secretes a transferrin with microheterogeneity of lectin affinity. | human ovarian clear cell carcinoma cell line (transferrin (tf)-non-producer), hac 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. they synthesized and secreted constantly the 80 kda protein immunologically similar to human serum tf (15 +/- 12 ng/ml/10(7) cells/3 days). by sensitive lectin-affinity electrophoresis followed by antibody-affinity blotting technique, a concanava ... | 1990 | 2226780 |
| rat brain n-acetylated alpha-linked acidic dipeptidase activity. purification and immunologic characterization. | n-acetylated alpha-linked acidic dipeptidase (naala dipeptidase) is a membrane-bound metallopeptidase that cleaves glutamate from the endogenous neuropeptide n-acetyl-l-aspartyl-l-glutamate. in this report, we have solubilized naala dipeptidase activity from synaptosomal membranes with triton x-100 and purified it to apparent homogeneity by sequential column chromatography on deae-sepharose, cm-sepharose, and lentil lectin-sepharose. this procedure resulted in a 720-fold purification with 1.6% y ... | 1990 | 2250024 |
| ontogenesis of the secretory epithelium of the bovine subcommissural organ. a histofluorescence study using lectins and monoclonal antibodies. | a spatio-temporal analysis of the differentiation of a group of specialized (secretory) ependymal cells in the subcommissural organ (sco) of the brain was undertaken in the bovine using a monoclonal antibody (c1b8a8) which is specific of the secretory process in this organ. in addition, lectins (concanavalin agglutinin (con a), lens culinaris agglutinin (lca), wheat germ agglutinin (wga), and phaseolus vulgaris agglutinin (pha] were used to analyse the maturation of the carbohydrate moieties of ... | 1990 | 2253320 |
| high molecular weight soluble neutral maltase-glucoamylases in the intestine of the suckling rat. | previous work from our laboratory has shown that the intestine of the suckling rat, unlike adult rat intestine, contains abundant quantities of at least two soluble neutral maltase-glucoamylases. these enzymes are related antigenically to membrane-bound maltase-glucoamylase, which predominates in adult intestine, but are either more easily solubilized or occupy a different cellular locus. to study the soluble enzymes further, we attempted their isolation from the intestine of 11-day-old suckling ... | 1990 | 2257117 |
| identification of the surface polypeptide antigens of the infective larvae of onchocerca volvulus. | the surface polypeptide antigens of third-stage infective larvae (l3) and adult males of onchocerca volvulus have been compared after iodogen-catalysed radioiodination, separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in beta-mercaptoethanol followed by autoradiography. l3 surfaces contained polypeptides with apparent molecular weights of 14, 18.5, 26, 34, 51, 68 and 130 kda, whereas the adults contained 14, 19, 26, 34, 37.5 and 68 kda components. by immunoprecipitation w ... | 1990 | 2278076 |
| recombinant carboxyl-terminal fibrin-binding domain of human fibronectin expressed in mouse l cells. | the carboxyl-terminal fibrin-binding domain, fib2, of human fibronectin was expressed in mouse l cells as a fusion protein with the signal sequence of human protein c inhibitor. the recombinant fib2 (rfib2) protein synthesized by transfected cells retained the ability to form dimers with each other or with mouse fibronectin subunits and was secreted to the medium after extensive glycosylation. only a small fraction of the secreted protein was incorporated into the pericellular matrix. interestin ... | 1990 | 2294110 |
| distribution of cell surface glycoconjugates during secondary neurulation in the chick embryo. | lectin histochemistry was used to examine the expression of cell surface glycoconjugates during secondary neurulation in chick embryos. fourteen lectins were applied to serial sections of the caudal region of embryos at the various stages of tail bud development. the lectins bandeiraea simplicifolia, dolichos biflorus agglutinin, phaseolus vulgaris leukoagglutinin, soybean agglutinin, sophora japonica agglutinin, ulex europaeus agglutinin and succinylated wheat germ agglutinin (swga) showed very ... | 1990 | 2297085 |
| secretory glycoconjugates of a mucin-synthesizing human colonic adenocarcinoma cell line. analysis using double labeling with lectins. | lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. despite being clonally derived, ht29-18n2 (n2) cells, like normal goblet cells in situ were heterogeneous in their glycosylation of mucin. only wheat-germ agglutinin, which recognizes n-acetylglucosamine and sialic acid residues, and succinylated wheatgerm agglutinin, which binds n-acetylglucosamine, stain ... | 1990 | 2312359 |
| membrane-bound and water-soluble nonclassical class i mhc antigens on rat placenta. | we have used mouse monoclonal antibodies to different determinants on rat class i major histocompatibility complex (mhc) antigens in order to identify water-soluble and membrane-bound nonclassical (i. e., non-rt1.a) class i mhc antigens on the spongiotrophoblast and labyrinthine trophoblast of rat placenta. initial immunohistological studies with monoclonal antibodies reacting with a determinant restricted to classical (rt1.a) rat class i antigens confirmed the presence of these antigens on spon ... | 1990 | 2318515 |
| duck lymphocytes--iii. transformation responses to some common mitogens. | the lymphocyte transformation (lt) test was performed using duck blood lymphocytes stimulated with phytohaemagglutinin (pha), concanavalin a (con a), lentil lectin (lc), roman snail lectin (hp), peanut agglutinin (pna), bandeiraea simplicifolia seed lectin (bss), wheat germ agglutinin (wga), horseshoe crab lectin (hsc), pokeweed mitogen (pwm) and e. coli lipopolysaccharide (lps). cells were cultured in microtitre trays, at 41.6 degrees c, 8 x 10(5) cells in 200 microliters medium (= 4 x 10(6) ce ... | 1990 | 2323180 |
| neurothelin: an inducible cell surface glycoprotein of blood-brain barrier-specific endothelial cells and distinct neurons. | the blood-brain barrier is characterized by still poorly understood barrier and transport functions performed by specialized endothelial cells. hybridoma technology has been used to identify a protein termed neurothelin that is specific for these endothelial cells. neurothelin is defined by the species-specific mouse mab 1w5 raised against lentil-lectin-binding proteins of neural tissue from embryonic chick. in the posthatch chick, neurothelin expression is found on endothelial cells within the ... | 1990 | 2324198 |
| lectin-resistant variants of mouse lewis lung carcinoma cells. ii. altered glycosylation of membrane glycoproteins. | lectin-resistant variants of mouse lewis lung carcinoma ll2 cell line, selected with wheat germ agglutinin (wgar), ricinus communis agglutinin ii (rca iir) and aleuria aurantia agglutinin (aaar) were studied. total cellular glycopeptides of the parent ll2 line and of the five lectin-resistant variants were analyzed by gel filtration and affinity chromatography on immobilized concanavalin a and lens culinaris agglutinin. the results revealed that low-metastatic wgar and rca iir variants possessed ... | 1990 | 2328549 |
| lectin staining of saccharides in the normal and hypothyroid developing organ of corti. | lectin staining has been used to detect mono- and oligosaccharides in normal and hypothyroid developing organs of corti in the rat. eight developmental stages were studied (1, 5, 8, 10, 15, 20, 50 and 60 days after birth). congenital hypothyroidism was induced by oral administration of propylthyouracil to pregnant rats. labelling of the tectorial membrane with 3 lectins, ulex europaeus agglutinin-i (uea-i), lens culinaris agglutinin (lca) and ricinus communis agglutinin-i (rca-i) showed no signi ... | 1990 | 2331782 |
| structure of asparagine-linked oligosaccharides on human and rabbit testosterone-binding globulin. | we have previously demonstrated, using two-dimensional polyacrylamide gel electrophoresis, that much of the microheterogeneity of human (h) and rabbit (rb) testosterone-binding globulin (tebg) is due to differential glycosylation of a single protomer. since glycosylation has been shown to be a physiologically important modification of proteins, we have examined the structure of the oligosaccharide chains attached to htebg and rbtebg to facilitate future studies on the mechanisms of action of the ... | 1990 | 2340332 |
| development of cortical vesicles in sicyonia ingentis ova: their heterogeneity and role in elaboration of the hatching envelope. | in the marine shrimp sicyonia ingentis, ova lack cortical vesicles at spawning. previous ultrastructural studies suggested that two different populations of cortical vesicles (dense vesicles and the ring vesicles) appear within 30 min post-spawning. these vesicles undergo sequential exocytosis (exocytosis of the dense vesicles followed by exocytosis of the ring vesicles) that leads to the formation of a hatching envelope around the ovum (see pillai and clark: tissue & cell 20:941-52, 1988). in t ... | 1990 | 2346649 |
| stimulation by concanavalin a of cartilage-matrix proteoglycan synthesis in chondrocyte cultures. | the effect of concanavalin a on proteoglycan synthesis by rabbit costal and articular chondrocytes was examined. chondrocytes were seeded at low density and grown to confluency in medium supplemented with 10% fetal bovine serum, and then the serum concentration was reduced to 0.3%. at the low serum concentration, chondrocytes adopted a fibroblastic morphology. addition of concanavalin a to the culture medium induced a morphologic alteration of the fibroblastic cells to spherical chondrocytes and ... | 1990 | 2351653 |
| effect of pea and lentil lectins on in vitro absorption of nutrients. | in vitro absorption of nutrients like glucose, leucine, protein hydrolysate and ca2+ by ligated loops of small intestine was significantly affected in presence of lectins from peas and lentils. except for sucrose, all other nutrients showed significant decrease in their absorption in presence of lectins. lentil lectins had a greater inhibitory effect than pea lectins. | 1990 | 2365412 |
| in vitro effect of lectins from peas (pisum sativum) and lentils (lens culinaris) on lipid peroxidation and superoxide dismutase activity in normal and vitamin b6 deficient albino rats. | 1990 | 2365414 | |
| analysis of the oligosaccharides on rat androgen-binding protein using serial lectin chromatography. | the microheterogeneity seen when rat androgen-binding protein (rabp) is analyzed by two-dimensional polyacrylamide gel electrophoresis is attributable, at least in part, to the differential glycosylation of a single promoter. further insight into the chemical nature of the oligosaccharide units on rabp was obtained by serial lectin chromatography. when rabp was chromatographed on immobilized concanavalin a (con-a), it was fractionated into three classes: (1) one that did not bind to the lectin ( ... | 1990 | 2378933 |
| inhibition of fibrin(ogen) binding to stimulated platelets by a monoclonal antibody specific for a conformational determinant of gpiiia. | we have purified the integrin gpiib:iiia from the membrane fraction of human blood platelets by lentil lectin affinity chromatography followed by gel filtration chromatography. with purified gpiib:iiia as an antigen, we have produced monoclonal antibody cs-1, which immunoblotting demonstrates to be specific for native gpiiia; disulfide bond reduction of gpiiia resulted in loss of immunoreactivity. radiolabelled ligand binding studies revealed that cs-1 recognized approximately 55,000 sites per p ... | 1990 | 2385828 |
| heterogeneity in lectin-binding characteristics of human lymphokine-activated killer cells. | the binding of various lectins to human lymphokine-activated killer (lak) cells was investigated. human peripheral blood lymphocytes were cultured for 3 days with recombinant interleukin-2 (ril-2), and then stained with 20 kinds of lectins. there were four types of lectin-binding patterns: (a) negative staining; (b) weakly positive staining; (c) strongly positive staining; and (d) two populations, one weakly and one strongly positive staining. among the 20 kinds of lectins, both lens culinaris a ... | 1990 | 2395003 |
| glycoprotein storage in gaucher disease: lectin histochemistry and biochemical studies. | lectin histochemical studies were performed on formalin-fixed, frozen, and paraffin-embedded tissue sections from 19 patients with glucosylceramide lipidosis (i.e., gaucher disease). eleven different lectins were used to identify the specific carbohydrate residues in the undegraded stored compounds in the cytoplasm of gaucher cells. in all cases studied, gaucher cells stained with concanavalia ensiformis agglutinin, datura stramonium agglutinin, lens culinaris, ricinus communis agglutinin-i, and ... | 1990 | 2395332 |
| presence of an antigen-specific t cell subset that forms ige-suppressive factor and igg-suppressive factor on antigenic stimulation. | b6d2f1 mice were given three i.v. injections of ovalbumin (oa), and antigen-specific t cell clones were established from their spleen cells. one of the fcr+ t cell clones formed ige-binding factors on incubation with oa-pulsed syngeneic macrophages. neither soluble antigen nor macrophages alone induced factor formation. t cell hybridomas were constructed by fusion of the antigen-specific t cell clone with bw 5147 cells. among 11 t cell hybridomas established, six clones produced ige-binding fact ... | 1985 | 2409160 |
| transneuronal transport of lectins. | axonal and transneuronal transport of the plant lectins wheat germ agglutinin (wga), pisum sativum agglutinin (psa), lens culinaris agglutinin (lca), soybean agglutinin (sba), peanut agglutinin (pna), concanavalin a agglutinin (con a), and ulex europeus agglutinin (uea) were examined and compared using an immunocytochemical staining method. wga, which binds to n-acetylglucosamine and sialic acid carbohydrate residues, and the 3 mannose binding lectins (con a, psa and lca) were found to undergo r ... | 1985 | 2412649 |
| alpha-fetoprotein subfractions in amniotic fluid identified by a modification of the method of concanavalin a, lentil lectin or phytohemagglutinin-e affinity crossed-line immunoelectrophoresis. | using a modified method of concanavalin a (con a), lentil lectin (lch) or phytohemagglutinin-e (pha-e) affinity crossed-line immunoelectrophoresis, alpha-fetoprotein (afp) subfractions were studied in 33 samples of human amniotic fluid obtained between 41 and 287 days of gestation. fetal tissues (yolk sac, liver, stomach and small intestine) obtained from a fetus of 68 days' gestation were incubated for 24 hours and afp subfractions in the culture fluid examined. afp in control amniotic fluids y ... | 1985 | 2416031 |
| characterization of the human glomerular c3 receptor as the c3b/c4b complement type one (cr1) receptor. | the functional and immunochemical characteristics of the human glomerular c3 receptor were investigated by adherence of sheep erythrocytes (es) coated with defined c3 fragments and by using polyclonal and/or monoclonal antibodies directed against epitopes expressed on complement receptors cr1, cr2, and cr3. c3b-bearing es (esc3b) strongly adhered to glomeruli in frozen kidney sections in a reaction that was selectively inhibited by f(ab')2 anti-cr1 antibodies. there was no adherence of esc3dg, e ... | 1986 | 2418113 |
| biochemical and immunologic characterization of a major surface antigen of dirofilaria immitis infective larvae. | a 35 kd major surface antigen of dirofilaria immitis third-stage larvae was characterized biochemically and immunologically. living larvae were iodinated by using iodo-gen, iodosulfanilic acid, lactoperoxidase-glucose oxidase, and bolton-hunter reagents. detergent extracts of larvae labeled by the first three methods showed one major 35 kd component and a number of smaller components of about 6 kd, as analyzed by one-dimensional sds-page. in contrast, extracts from larvae labeled with the bolton ... | 1986 | 2419445 |
| serum alpha-fetoprotein subfractions in pregnant women identified by the modified method of lectin affinity crossed-line immunoelectrophoresis. | using a modified method of lectin affinity crossed-line immunoelectrophoresis, we studied the alpha-fetoprotein (afp) subfractions in sera from 12 pregnant japanese women, at 22 and 42 weeks of gestation. the method involves the first dimension electrophoresis in agarose gel containing concanavalin a (con a) or lentil lectin (lch), the second dimension immunoelectrophoresis in agarose gel containing polyclonal antibody against afp, reaction with peroxidase-conjugated protein a and staining with ... | 2008 | 2423145 |
| localization of lectin binding sites in human, cat, and rabbit corneas. | paraffin sections of human, cat, and rabbit corneas were stained with nine lectins, using an avidin-biotin-complex procedure to study glycoconjugates of the epithelium, keratocytes, and stromal matrix. wheat germ agglutinin (wga) stained plasma membranes of all epithelial cell layers of cat and human and superficial and wing cells of rabbit. plasma membranes of superficial and wing cells of cat epithelium also stained with peanut agglutinin (pna) and ricinus communis agglutinin i (rca-i). human ... | 1986 | 2426217 |
| domain-specific distribution of carbohydrates in human fibronectins and the transformation-dependent translocation of branched type 2 chain defined by monoclonal antibody c6. | fibronectins purified from human plasma (termed pfn), spent culture media of human fibroblasts wi38 (termed cfn), and sv40 virus-transformed wi38/va13 cells (termed tfn) and their cleavage fragments were compared with respect to their binding activities to lectins and anti-carbohydrate antibodies reacting with chemically well-defined structures. the following findings were of particular interest. about 25-35% of cfn and tfn carried a binary sialosyl type 2 chain (neuac alpha 2----3/or 6gal beta ... | 1986 | 2426270 |
| further resolution of human alpha-fetoprotein by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin. | major molecular species of human alpha-fetoprotein(afp), which were separated as single components by serial affinity chromatography with concanavalin a(con-a) and lens culinaris agglutinin, were further resolved into several bands by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin(e-pha). among the newly separated main molecular species, both con-a- and e-pha-reactive afp(afp-1x1) was demonstrated, contrary to the known sugar specificity of con ... | 1985 | 2426755 |
| [differential diagnosis of benign liver diseases from primary liver cancer with elevation of afp shown by lentil lectin immunoelectrophoresis]. | 1986 | 2427282 | |
| structural implications of the location and stability to proteolytic enzymes of immunodominant determinants of the human leukocyte common molecule. | in this report, we use 8 different mouse monoclonal antibodies to define 3 immunodominant determinants of the human leukocyte common (lc) antigen, and by defining the relative location and the stability to proteolytic enzymes of these determinants we make some structural predictions about the lc family of molecules. one lineage-restricted determinant is expressed predominantly on b lymphocytes and is located on the two higher (210 and 195) kda bands of lc, with possibly some expression on the 18 ... | 1986 | 2427343 |
| two chinese hamster ovary glycosylation mutants affected in the conversion of gdp-mannose to gdp-fucose. | a biochemical basis for the pea and lentil lectin resistance of two chinese hamster ovary (cho) cell mutants, lec13 and lec13a, was investigated. studies of the g glycopeptides of vesicular stomatitis virus grown in the mutants indicated that lec13 cells essentially lack the ability to add fucose to complex carbohydrates while lec13a cells synthesize significant proportions of fucosylated, complex moieties. however, both mutants were known to be reverted to lectin sensitivity by growth in l-fuco ... | 1986 | 2428310 |
| establishment and characterization of a human ovarian endodermal sinus tumor cell line--producing specific type of alpha-fetoprotein subfraction. | a cell line was established from an endodermal sinus tumor of the ovary from a 57-year-old japanese woman, obtained on july 22, 1982. histological study of the resected tumor revealed a mixture of reticular, solid and cystic patterns, being consistent with the characteristic feature. the cell line, designated as human akimoto endodermal sinus tumor (haest), was subcultivated over 50 times during 29 months. the cells were spindle or columnar in shape and showed pleomorphic and neoplastic features ... | 1986 | 2430863 |
| close topographical relationship in alpha foetoprotein (afp) between a lens culinaris binding glycan and the epitope recognized by afp-reactive monoclonal antibody, 18h4. | monoclonal antibodies 18h4 and 19f12 against alpha-foetoprotein (afp) were examined by enzyme immunoassay for binding to two forms of afp that were separated on the basis of the reactivity with lentil lectin (lca). lca-binding and lca non-binding afp, coated on a solid phase, reacted with 18h4 but reactivity with the lca-binding species was inhibited by 60% following pretreatment of the afp with lca. the lectin was a very poor inhibitor of binding of 18h4 to the afp which did not interact with l ... | 1987 | 2434121 |
| monoclonal antibody specific for t cell-derived human ige binding factors. | a b cell hybridoma secreting monoclonal antibody against human ige binding factors was obtained by immunization of balb/c mice with partially purified ige binding factors, and fusion of their spleen cells with sp-2/0-ag14 cells. the monoclonal antibody bound all of the 60,000, 30,000, and 15,000 dalton ige binding factors from two t cell hybridomas and those from activated t cells of a normal individual. the antibody bound both ige-potentiating factors, which had affinity for lentil lectin, and ... | 1987 | 2437189 |
| subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle. | purified dihydropyridine-sensitive calcium channels from rabbit transverse-tubule membranes consist of three noncovalently associated classes of subunits: alpha (167 kda), beta (54 kda), and gamma (30 kda). cleavage of disulfide bonds reveals two distinct alpha polypeptides and an additional component, delta. the alpha 1 subunit, a 175-kda polypeptide that is not n-glycosylated, contains the dihydropyridine binding site, camp-dependent protein kinase phosphorylation site(s), and substantial hydr ... | 1987 | 2440051 |
| an arg-gly-asp-directed receptor on the surface of human melanoma cells exists in an divalent cation-dependent functional complex with the disialoganglioside gd2. | the disialogangliosides gd2 and gd3 play a major role in the ability of human melanoma cells to attach to arg-gly-asp-containing substrates such as fibronectin and vitronectin, since pretreatment of these cells with monoclonal antibodies to the oligosaccharide of gd2 and gd3 can inhibit their attachment and spreading on such adhesive proteins. this report demonstrates that human melanoma cells (m21) synthesize and express a glycoprotein receptor that shares antigenic epitopes with the vitronecti ... | 1987 | 2443507 |
| class 1 (unique) tumor antigens of human melanoma: partial purification and characterization of the fd antigen and analysis of a mouse polyclonal antiserum. | serum antibodies from melanoma patient fd were previously shown to detect a unique antigenic specificity (fd) expressed only on the autologous tumor cells (sk-mel-131) in culture. the fd determinant is carried by a mr 90,000 glycoprotein (gp90) that binds to concanavalin a but not to lentil lectin or wheat germ agglutinin. after treatment with endo-n-acetylglucosaminidase h, the antigen no longer bound to concanavalin a. these and other properties indicate the gp90 carries mainly high mannose or ... | 1987 | 2445475 |
| an immunoglobulin m monoclonal antibody, recognizing a subset of acetylcholinesterase molecules from electric organs of electrophorus and torpedo, belongs to the hnk-1 anti-carbohydrate family. | an immunoglobulin m (igm) monoclonal antibody (mab elec-39), obtained against asymmetric acetylcholinesterase (ache) from electrophorus electric organs, also reacts with a fraction of globular ache (amphiphilic g2 form) from torpedo electric organs. this antibody does not react with asymmetric ache from torpedo electric organs or with the enzyme from other tissues of electrophorus or torpedo. the corresponding epitope is removed by endoglycosidase f, showing that it is a carbohydrate. the subset ... | 1987 | 2445915 |
| reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells. | the importance of glycoproteins located in the nuclear envelope in nuclear transport was tested by microinjection of karyophilic proteins into the cytoplasm of cultured human cells together with various lectins. wheat germ agglutinin (wga) blocked the nuclear transport of nucleoplasmin, a nuclear protein of xenopus laevis oocytes, and of nonnuclear proteins conjugated with a synthetic peptide containing the nuclear localization signal sequence for simian virus 40 (sv40) large t antigen. its inhi ... | 1987 | 2446896 |
| structural and functional analysis of cr2/ebv receptor by means of monoclonal antibodies and limited tryptic digestion. | the receptor for the c3d fragment of the third component of complement, cr2, has recently been shown also to act as the receptor for the epstein-barr virus (ebv) and to be involved in the control of b-cell proliferation. in order to define functionally important domains on this molecule, we produced monoclonal antibodies to several distinct epitopes. cr2 was purified from a np-40 lysate of human tonsils by a new method involving sequential chromatography on lentil lectin sepharose 4b and deae-se ... | 1988 | 2448232 |
| selective staining of platelet glycoproteins using two-dimensional o'farrell gel electrophoresis and avidin-biotin-conjugated lectins. | a time-saving and sensitive high resolution method for the analysis and identification of platelet glycoproteins using nonradioactive compounds has been developed. platelet proteins (50 micrograms) of normal subjects were separated by isoelectric focusing and sds-page. proteins were either stained with silver or electroblotted onto nitrocellulose. nitrocellulose blots were treated with the following biotinylated lectins: abrus precatorius lectin, concanavalin a, lens culinaris lectin, or wheat g ... | 1987 | 2451305 |
| expression of thy-1 and effect of phosphatidylinositol-specific phospholipase c on primate and murine cell lines. | balc/c mice were immunized with isolated human brain thy-1. the antisera at an appropriate dilution only reacted with a doublet of an apparent molecular weight (mw) around 25,000 among all the glycoproteins of brain tissue isolated by lentil lectin affinity chromatography when tested by enzyme-linked immunosorbent assay and immunoblotting. when the antisera were used to test a number of human cell lines and a marmoset t-cell line (70n2) by flow cytometry, an astrocytoma cell line (u-373), a t-ly ... | 1988 | 2451568 |
| tissue of origin of elevated alpha-fetoprotein in ataxia-telangiectasia. | although only a single gene exists for alpha-fetoprotein (afp), differential glycosylation generates several different forms and these are associated with different tissues of origin, namely, liver, gastrointestinal tract, and yolk sac. this microheterogeneity of serum afp was studied in seven patients with ataxia-telangiectasia (at) in order to determine the tissue of origin of their elevated afp levels. concanavalin a (con a), lens culinaris agglutinin a (lca-a), and erythroagglutinating phyto ... | 1986 | 2454778 |
| leishmania donovani: identification of glycoproteins released by promastigotes during growth in vitro. | culture supernatants of metabolically labeled leishmania donovani promastigotes were shown to contain approximately 40 electrophoretically distinct released protein compounds. of these, approximately 20 were glycoproteins which contained terminal mannose residues, as judged by their specific binding to concanavalin a-agarose beads. smaller subsets of the released glycoproteins were bound by agarose-conjugated lens culinaris, ricinus communis, and peanut lectins. promastigote mannose-containing r ... | 1988 | 2461315 |
| glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins. | the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ... | 1988 | 2466021 |
| further characterization of monoclonal antibody 6,f-8 reacting with lathyrus, lens and pisum lectins. | the murine monoclonal antibody (moab) 6,f-8 made against the glucose/mannose-specific lathyrus odoratus mitogen has previously been shown to react with lens culinaris and pisum sativum lectins, but not with the lectin from vicia faba [(1988) biol. chem. hoppe-seyler 369, 365-370]. the reactivity against seven other completely sequenced lathyrus lectins has now been tested after separation of the subunits by sds-polyacrylamide gel electrophoresis and electroblotting to nitrocellulose filters. two ... | 1989 | 2468535 |
| human melanoma cell attachment involves an arg-gly-asp-directed adhesion receptor and the disialoganglioside gd2. | the disialogangliosides gd2 and gd3 play a major role in the ability of human melanoma cells to attach to arg-gly-asp-containing substrates such as fibronectin and vitronectin, since pretreatment of these cells with monoclonal antibodies to the oligosaccharide of gd2 and gd3 can inhibit their attachment and spreading on such adhesive proteins. this report demonstrates that human melanoma cells (m21) synthesize and express a glycoprotein receptor that shares antigenic epitopes with the vitronecti ... | 1989 | 2470110 |
| binding of lectins to human leukaemic cells. | the interactions of five different lectins: peanut (pna), lentil (len), wheat germ (wga), soybean (sba), asparagus pea (fbp) with leukaemic cells obtained from 31 children: 25 with acute lymphoblastic leukaemia (all) and 6 with acute myeloblastic leukaemia (aml) were examined in this study. the relationship of lectin-binding ability to cells cytomorphological, cytochemical and immunological features and its potential clinical application were investigated. it has been shown that pna and len rece ... | 1989 | 2475405 |
| carbohydrate composition of beta-core. | beta-core is a major component of the hcg-related molecules found in pregnancy urine. we previously have purified the beta-core molecule and have deduced portions of its carbohydrate structure based on lectin binding data. in the present study we used recently developed technology to determine the carbohydrate composition of beta-core and hcg beta (cr119). for direct compositional analysis, parallel samples were hydrolyzed in trifluoroacetic acid and analyzed for sialic acid and neutral sugars w ... | 1989 | 2477227 |
| lectin affinity electrophoresis of alpha-fetoprotein in cancer diagnosis. | lectin affinity electrophoresis of serum alpha-fetoprotein (afp) was carried out on samples obtained from patients with benign and malignant diseases and on cord blood, and separated afp bands were detected by antibody-affinity blotting. the following major bands were identified by determination of kinetic constants: afp-c1 and -c2 with concanavalin a, afp-l1, -l2 and -l3 with lens culinaris agglutinin a, afp-p1, -p2, -p3, -p4 and -p5 with erythroagglutinating phytohemagglutinin, and afp-a1, -a2 ... | 2007 | 2478360 |
| affinity electrophoresis for diagnosis of cancer and inflammatory conditions. | affinity electrophoresis, with concanavalin a and lens culinaris agglutinin as ligands, was applied to study the microheterogeneity of serum proteins, with special emphasis on alpha 1-fetoprotein and alpha 1-acid glycoprotein, two proteins of potential clinical value. a total of 602 samples of serum from patients with various neoplastic and inflammatory conditions were evaluated. affinity electrophoresis provided useful information for differential diagnosis of cancers of various origins includi ... | 2006 | 2478361 |
| cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse. | the expression of the extracellular matrix molecules cytotactin, which is synthesized by glia, and cytotactin-binding (ctb) proteoglycan, which is synthesized by neurons, was examined in the developing brain of the mouse, specifically in the cortical barrel field, using highly specific polyclonal antibodies to the purified molecules. both molecules appeared early in the development of the cortex but were excluded from the centers of the developing barrels at the time of entry and arborization of ... | 1989 | 2479585 |
| two-dimensional lectin affinity electrophoresis of alpha-fetoprotein: characterization of erythroagglutinating phytohemagglutinin-dependent microheterogeneity forms. | by means of two-dimensional lectin affinity electrophoresis of human alpha-fetoprotein (afp) from different sources, afp bands separated with erythroagglutinating phytohemagglutinin (e-pha) were further characterized with other lectins of known oligosaccharide specificities. the results with a cord serum afp revealed that not only afp-p2 (e-pha-nonreactive) but also afp-p4 and p5 (e-pha-reactive) had affinities for concanavalin a (con a) and allomyrina dichotoma lectin (allo a), indicating that ... | 1989 | 2482176 |
| [electrophoretic analysis of urinary glycoproteins in diabetic nephropathy using peroxidase-lectins]. | we examined the clinical usefulness determined by polyacrylamide gel electrophoresis, followed by reaction with peroxidase-coupled lectins using urinary glycoproteins for diabetic nephropathy in 20 patients with diabetes mellitus. lectins used were triticum vulgaris (wga), phaseolus vulgaris (pha-e4), dolichos biflorus (dba), and lens culinaris (lca), which have high affinity for beta 1----4n-acetyl-d-glucosamine (glcnac beta 1----4glcnac), n-acetyl-d-galactosamine (galnac), alpha-galactosamine ... | 1989 | 2483179 |
| characterization and localization of the sporulation glucoamylase of saccharomyces cerevisiae. | glucoamylase (sga) was purified approximately 250-fold from sporulating saccharomyces cerevisiae cells. the partially purified enzyme was active against glycogen, starch, maltotriose and maltose. it exhibited maximum catalytic activity against glycogen at ph 5.5. the enzyme appears to be glycosylated, because it bound to lentil-lectin sepharose. sga was expressed in vegetatively growing cells under the control of the gal1 promoter, and the cellular location of the enzymatic activity determined b ... | 1989 | 2493265 |
| inhibin and activin-like activity in fluids from male and female gonads: different molecular weight forms and bioactivity/immunoactivity ratios. | the existence of various molecular weight forms of inhibin in ovarian follicular fluid has been reported earlier, while there is no information on the form of inhibin in testicular tissue. inhibin bioactivity was therefore estimated in eluates of slices, obtained after sds-page of rat testicular and ovarian homogenates, rat sertoli cell-conditioned medium (rsccm) and bovine ovarian follicular fluid (bff). the only form of inhibin detected in testes from 22-day-old rats and in rsccm was a 30 kda ... | 1989 | 2504871 |
| asparagine-linked glycosylation of the scrapie and cellular prion proteins. | post-translational modification of the scrapie prion protein (prp) is thought to account for the unusual features of this protein. molecular cloning of a prp cdna identified two potential asn-linked glycosylation sites. both the scrapie (prpsc) and cellular (prpc) isoforms were susceptible to digestion by peptide n-glycosidase f (pngase f) but resistant to endoglycosidase h as measured by migration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. pngase f digestion of prpc yielded t ... | 1989 | 2505674 |
| glycosylation characteristics of the mouse liver lactogenic receptor. | the structural characteristics and glycosylation properties of the lactogenic receptor were examined in 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (chaps)-solubilized plasma membranes from female mouse liver. the specific binding of the radioiodinated human growth hormone [( 125i]hgh) was displaced with an equivalent potency by both hgh and prolactin. after a mild neuraminidase treatment, this binding was increased by 40%, as a result of an increase in receptor affinity. affinity ... | 1989 | 2506088 |
| the notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during drosophila development. | the notch locus of drosophila melanogaster is one of a small number of zygotically acting "neurogenic" genes involved in the correct segregation of neural from epidermal lineages during embryogenesis as well as in other postembryonic developmental events. we have generated antibody probes against three regions of the notch protein to study the expression of notch and begin a biochemical characterization of the protein. consistent with predictions based on dna sequence data, here we gather eviden ... | 1989 | 2509485 |
| production of interferon-alpha and gamma by leukocytes from patients with schizophrenia. | production of interferon (ifn)-alpha and -gamma by leukocytes from 34 patients with acute schizophrenia and 34 controls was measured before and after 5-6 weeks of antipsychotic treatment by using sendai virus as ifn-alpha inducer and lentil lectin as inducer for ifn-gamma production. the schizophrenia series included 13 first admission patients (mean duration of illness 1.1 years) and 21 re-entry patients (mean duration of illness 10.1 years). of the total series 23 were drug-free at the time of ... | 1989 | 2518635 |
| molecular cloning and expression of cdna encoding the 53,000-dalton glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum. | the 53-kda glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum was purified by lentil lectin affinity chromatography and preparative polyacrylamide gel electrophoresis and partially sequenced. polyclonal and monoclonal antibodies were raised against the 53-kda glycoprotein and found to cross-react with the 160-kda glycoprotein. a combination of antibody and synthetic oligonucleotide screening was used to isolate a cdna encoding the 53-kda glycoprotein of rabbit fast-twitch skeletal mus ... | 1989 | 2521635 |
| isolation and characterization of complement receptors cr1 from human peripheral nerves. | extracts from myelinated and unmyelinated nerves, prepared using nonidet p-40, contained receptors for c3b/c4b (cr1). extracts from myelinated nerves inhibited eac3b rosette formation with peripheral blood leucocytes and agglutinated eac3b, whereas extract from unmyelinated nerves did not. rosette formation with eac3bi or eac3d was not affected. cr1 in extracts from myelinated nerves also expressed decay-accelerating activity of the alternative pathway c3 convertase and cofactor activity in fact ... | 1989 | 2526822 |
| primary structure of the major o-glycosidically linked carbohydrate unit of human von willebrand factor. | a reduced tetrasaccharide chain was obtained from human von willebrand factor (vwf) by mild alkaline borohydride treatment. the purification of this o-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin a and lens culinaris agglutinin and finally gel filtration. its structure was determined by a combination of methylation studies and 500 mhz 1h-nmr spectroscopy to be: neuac(alpha 2-3)gal(beta 1-3)[neuac(alpha 2-6)]galnac-ol. | 1989 | 2535488 |
| production of antibodies against the coenzyme pyrrolequinoline quinone. | polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. these antibodies react with free and protein-bound pyrrolequinoline quinone. in particular they react with native and denatured lentil seedling amine oxidase as detected by dot-blot and elisa assays. the presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method. | 1989 | 2541012 |
| affinity cytochemical differentiation of glycoconjugates of small intestinal absorptive cells using pisum sativum and lens culinaris lectins. | we studied the subcellular localization of glycoconjugates recognized by the garden pea and lentil lectins (pisum sativum, psa; lens culinaris, lca) in mature absorptive cells of duodenum and jejunum of fasted rats. psa and lca are mannose-, glucose-, and n-acetyl-glucosamine-recognizing lectins that bind with high affinity to fucosylated core regions of n-glycosidically linked glycans. the binding reactions were cytochemically demonstrated in a pre-embedment incubation system using peroxidase-l ... | 1989 | 2542394 |
| a collagen-binding glycoprotein from bovine platelets is identical to propolypeptide of von willebrand factor. | several proteins from bovine platelet lysate bound to type i collagen immobilized to the beads of formyl derivatives of cellulose. among these proteins, a protein of about 100,000 daltons was purified to homogeneity by two additional affinity chromatographies, an organomercurial-agarose and a lentil lectin-agarose. this protein consisted of a single polypeptide chain which contains carbohydrate moiety and many intrapolypeptide disulfide bridges. in addition to platelets, this protein was present ... | 1989 | 2543668 |
| strong-cation-exchange sulfoethyl aspartamide chromatography for peptide mapping of staphylococcus aureus v8 protein digests. | in two recent reports (d. l. crimmins, j. gorka, r. s. thoma, and b. d. schwartz (1988) j. chromatogr. 443, 63-71; a. j. alpert and p. c. andrews (1988) j. chromatogr. 443, 85-96) a sulfoethyl aspartamide column was shown to efficiently analyze peptides less than 25 residues in length which differ in the number of nominal positive charges at ph 3.0. in particular, the elution order for a series of distinct peptides ranging in nominal charge from +1 to +7 was found to be monotonic in nature indic ... | 1989 | 2545105 |
| human gelatinase/type iv procollagenase is a regular plasma component. | gelatin zymograms revealed in human plasma a constant 66 kda proteolytically active polypeptide. in most plasma samples other major proteolytic activities were seen at mr 92,000, 130,000 and 225,000. all four proteases were ca2+-dependent metalloproteases and bound quantitatively to gelatin-sepharose. immunoblotting results indicated that the 66 kda protease was the human fibroblast gelatinase/type iv procollagenase and that the other three proteases were macrophage/granulocyte-derived gelatinas ... | 1989 | 2551735 |
| lectin binding to the porcine and human ileal receptor of intrinsic factor-cobalamin. | the purified porcine receptor for the intrinsic factor-cobalamin complex bound to concanavalin a, lentil lectin and wheat germ lectin covalently coupled to sepharose and was eluted with the corresponding soluble sugars. in contrast, human intrinsic factor bound efficiently to concanavalin a, to some extent to lentil lectin, but only slightly to wheat germ agglutinin. the binding of if-cbl to the receptor was inhibited when the receptor was pre-incubated with soluble wheat germ agglutinin, with a ... | 1989 | 2562509 |