Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| organization of the boar spermatozoan plasma membrane: evidence for separate domains (subdomains) of integral membrane proteins in the plasma membrane overlying the principal segment of the acrosome. | indirect immunofluorescence microscopy and freeze-fracture have been used to identify overlapping subdomains at the peripheral rim of the sperm-head plasma membrane (pm) and the margin of the outer acrosomal membrane (oam) comprising the principal segment of the acrosome of the boar spermatozoon. an array of ridge-like structures (spaced 12-16 nm centre-to-centre), originally observed on the oam by aguas & pinto da silva, lies just beneath an area of the pm that is sparsely populated with large ... | 1987 | 3329199 |
| the biosynthesis of sulfoquinovosyldiacylglycerol: studies with groundnut (arachis hypogaea) leaves. | the biosynthetic pathway of sulfoquinovosyldiacylglycerol (sqdg) was investigated using groundnut (arachis hypogaea) leaf discs and 35s-labeled precursors. [35s]so4(2-) was actively taken up by the leaf discs and rapidly incorporated into sqdg. after 2 h, 1.5% of the [35s]so4(2-) added to the incubation medium was taken up, of which 28% was incorporated into sqdg. the methanol-water phases of the lipid extracts of the leaf discs were analyzed for the 35s-labeled intermediates. up to 2 h of incub ... | 1988 | 3341737 |
| lectin expression in carcinoid tumours of the gastrointestinal tract. | the binding of peroxidase-conjugated dolichos biflorus (dba), triticum vulgaris (wga), canavalia ensiformis (con a), arachis hypogaea (pna), lotus tetragonolobus, and bandeiraea simplicifolia i (bsai) to gastrointestinal carcinoid tumours was studied. the results indicate that carcinoid tumour cells express certain carbohydrates similar to those present in the adjacent surface epithelium. the differences in the lectin-binding properties of carcinoid tumours of different sites of the gastrointest ... | 1988 | 3351672 |
| lectin binding activity in appendiceal mucocele. | in order to further clarify the histologic criteria and prognostic measurement of appendiceal adenoma and well differentiated adenocarcinoma, lectin binding activity in 31 cases of appendiceal mucocele (6 hyperplasias, 16 adenomas and 9 adenocarcinomas) was studied using an immunoperoxidase staining method. an additional 45 cases of colonic lesions (15 adenomas and 30 adenocarcinomas) were also examined as the corresponding controls. arachis hypogaea agglutinin (pna) bound selectively to the neo ... | 1988 | 3354500 |
| comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin. | 1. the amount of sialoglycopeptide (sgp) mixture released from young (y) erythrocytes by trypsin treatment was significantly larger than that from old (o) ones. 2. the two main membrane-surface sialoglycopeptides (y-sgp and o-sgp) were isolated in yields of about 45% and 42% from the mixtures by a combination of sephadex g-50 superfine gel filtration with deae-cellulose chromatography, respectively. molecular weights of 12,000 and 12,100, respectively, were found for the y-sgp and the o-sgp. 3. ... | 1988 | 3356136 |
| exposure of cryptantigens on erythrocytes in patients with breast cancer. | one hundred thirty-two patients with breast cancer were examined for exposure of cryptantigens on their erythrocytes (rbc) using a lectin panel consisting of arachis hypogaea and glycine soja. eight had exposed cryptantigens; of the eight, five were classified with additional lectins as t-polyagglutination type and three as th-polyagglutination. a control group of 300 healthy blood donors had no exposed cryptantigens on their rbc. these findings could not be correlated with the staging of the tu ... | 1988 | 3365668 |
| haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family. | twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ... | 1988 | 3377987 |
| separable anti-t and anti-tk lectins from the seeds of vicia hyrcanica. | separable anti-t and anti-tk lectins can be extracted from the seeds of vicia hyrcanica. the anti-t lectin is similar to that of the peanut, arachis hypogaea; the anti-tk lectin compares favourably with that of griffonia simplicifolia ii. | 1988 | 3388820 |
| glycoconjugate expression in human renal oncocytomas. a lectin histochemical study. | a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ... | 1988 | 3395218 |
| histochemical specificity of beta-galactose binding lectins from arachis hypogaea (peanut) and ricinus communis (castor bean). | previous histochemical studies have demonstrated disparities in the binding of two lectins with a nominal specificity for terminal beta-d-galactose. biochemical studies have shown that the most complementary structure for binding peanut agglutinin (pna) is the terminal disaccharide gal-(beta 1----3)-galnac, whereas the most complementary structure for binding ricinus communis agglutinin i (rca i) is the terminal disaccharide gal-(beta 1----4)-glcnac. however, it is not known if only these differ ... | 1987 | 3424394 |
| cytostructural localization of peanut lectin binding in fine-needle aspirates of benign and malignant breast lesions. | numerous studies have employed lectin binding patterns to investigate alterations in cell surface polysaccharide expression associated with malignant transformation. cytostructural relocalization of peanut lectin (arachis hypogaea) binding sites from the cell surface to the cytoplasm has been described in breast carcinomas. to investigate the possible utility of peanut lectin binding patterns in the diagnosis of breast fine-needle aspirations, the pna immunoperoxidase method was applied to smear ... | 1987 | 3428065 |
| specificity of fungal lipase in hydrolytic cleavage of oil. | lipase isolated from the fungus fusarium oxysporum f. sp. lini has shown specificity to saturated acids. in vitro hydrolytic splitting of cotton seed, ground-nut and fungal (f. oxysporum) oil substrates by the enzyme preparation from this fungus, revealed preferential fatty acid specificity. the enzyme attacked the triglyceride molecule, liberating 90-97% of saturated fatty acids, irrespective of length of the carbon chain. | 1987 | 3439435 |
| rapid lateral diffusion of lectin-labelled glycoconjugates in the human colonic adenocarcinoma cell line ht29. comparison with the synthetic lipid analogue dii-c14. | the lateral diffusion of lectin-labelled glycoconjugates was studied in the human colon carcinoma cell line ht29 using fluorescence photobleaching techniques. ht29 cells were grown in either dulbecco's modified eagle's medium with glucose (25 mm; dmem-glu) or with galactose (25 mm; dmem-gal). cell cultivation in the dmem-gal medium was assumed to promote a transformation of the cells to become small-intestinal-like with characteristic microvilli and associated enzymes. the diffusion of glycoconj ... | 1987 | 3484360 |
| lectin histochemical study on the infraorbital gland of the japanese serow (capricornis crispus). | histology and lectin histochemistry were performed in the infraorbital gland of the japanese serow. the gland is composed of glandular tissues and a pouch filled with the secretion. the tissues consist of an inner layer of sebaceous glands and an outer layer of apocrine glands. the male sebaceous layer is made up of the ordinary type, whereas the female's layer consists of the ordinary and modified types. in the apocrine gland stained with arachis hypogaea (pna), nine different patterns of gland ... | 1987 | 3506377 |
| lectin binding patterns in developing rat colon. | to evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. tissue from the proximal and distal colon of each animal was incubated with triticum vulgaris, arachis hypogaea, glycine max, or ulex europeus agglutinin i. formalin-fixed paraffin sections and unfixed frozen sections were coded, read blindly, and graded from nega ... | 1987 | 3557008 |
| evaluation of structural and secretory glycoconjugates in normal human jejunum by means of lectin histochemistry. | the labelling pattern of eight lectins was studied in jejunal samples from ten normal subjects, in order to define the normal distribution of structural and secretory glycoconjugates in the small bowel. the following lectins were studied by means of a peroxidase technique on formalin-fixed samples: arachis hypogaea, ricinus communis, canavalia ensiformis, lens culinaris, phaseolus vulgaris, triticum vulgaris, ulex europaeus, dolichos biflorus. phaseolus vulgaris reacted with goblet cell mucus th ... | 1987 | 3570880 |
| the structure of bowman-birk type protease inhibitor a-ii from peanut (arachis hypogaea) at 3.3 a resolution. | the structure of bowman-birk type protease inhibitor (a-ii from peanut) is described at 3.3 a resolution. the molecules form a tetramer with 222 local symmetry in our crystals. each monomer has an elongated shape with approximate dimensions of 45 x 15 x 15 a and consists of two distinct domains. the three-dimensional structures of the two domains are similar and are related by the intramolecular approximate twofold rotation axis. the two independent protease binding sites protrude from the molec ... | 1987 | 3571206 |
| mycoflora and mycotoxins of peanut (arachis hypogaea l.) seeds in egypt. 1--sugar fungi and natural occurrence of mycotoxins. | sixty-four species and 2 varieties which belong to nineteen genera of fungi were identified from 40 peanut seed samples collected from different places in egypt by using a dilution-plate method on glucose-czapek's medium. the most frequent genera were aspergillus (21 species & 2 varieties), penicillium (16 species) and fusarium (6 species). a. flavus, a. fumigatus, a. niger, p. chrysogenum and f. oxysporum were the most common fungal species. forty seven percent of the samples proved to be toxic ... | 1987 | 3587340 |
| glycoprotein allergens in pollen of timothy. v. significance of the carbohydrate moiety for the immunological activity of a basic glycoprotein allergen. | when the allergen was oxidized with periodate the size of its precipitate in rocket immunoelectrophoresis (rie) was reduced. incubation of the allergen with various glycosidases did not significantly affect its precipitation in rie. the binding of human ige and igg to allergen bound to nitrocellulose seemed not to be affected by incubation with alpha-l-fucosidase, beta-d-xylosidase, beta-d-galactosidase or periodate oxidation. incubation with alpha-d-mannosidase reduced the binding of igg to the ... | 1987 | 3596817 |
| histochemical characterization of glycoproteins present in jejunal and colonic goblet cells of pigs on different diets. a biopsy study using chemical methods and peroxidase-labelled lectins. | we examined the glycoprotein composition of intestinal goblet cells in jejunal and colonic biopsies obtained from pigs on different diets. paraffin sections were stained both chemically and with the following horseradish-peroxidase conjugated lectins: canavalia ensiformis (con-a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca1), glycine max (sba) and triticum vulgaris (wga). using chemical staining procedures, only small quantitative differen ... | 1987 | 3624000 |
| energy utilization of supplemented cereal diets in human volunteers. | energy utilization was studied in human volunteers using different diets containing wheat flour supplemented by groundnut (arachis hypogaea), "masur" (lens culinaris), mung (phaseolus aureus) and gram (cicer arietinum) flour. digestible and metabolizable energies were determined for all the experimental diets. an improved energy digestibility was observed when wheat flour was supplemented with groundnut flour, and groundnut flour plus gram flour, i.e. 93.35 and 89.48%, respectively. percent dige ... | 1986 | 3632213 |
| diabetes mellitus associated changes in glomerular glycocompounds: a fluorescence microscopical study. | renal biopsy specimens showing histological alterations typical of advanced diabetic glomerulopathy were studied for changes in glomerular glycoconjugates, using fluorochrome-coupled lectins as probes. all samples studied showed a marked reduction in the binding of triticum vulgaris (wga) lectin in the glomerular basement membranes. on the other hand, 'new' glomerular binding sites for the lectins of dolichos biflorus (dba), helix pomatia (hpa) and arachis hypogaea (pna), recognizing galactosyl ... | 2006 | 3667339 |
| separation of isolectins by high-performance hydrophobic interaction chromatography. | high-performance hydrophobic interaction chromatography (hp-hic) was found to be an effective method for the separation of lectins into isolectin fractions. all of the purified lectins used in this study, phaseolus vulgaris haemagglutinin (pha), wheat germ agglutinin (wga), ricinus communis agglutinin (rca), and arachis hypogaea agglutinin (aha), were prepared by affinity chromatography. hp-hic was performed on a column (15 x 2.1 cm) of tsk gel phenyl-5pw at room temperature. the lectin sample, ... | 1987 | 3667761 |
| intravascular [correction of intracellular] hemolysis and renal failure in a patient with t polyagglutination. | a patient with postoperative intravascular hemolysis aggravated by transfusion with fresh whole blood and fresh plasma products had acute renal failure. screening with arachis hypogaea and glycine soja lectins showed that his red cells were t-transformed. only washed red cells were transfused subsequently, and no further fresh plasma products were given. all hemolysis ceased, the renal function returned to normal, and the t-polyagglutination as measured by lectin tests disappeared 4 months after ... | 2015 | 3705141 |
| high-mr glycoprotein profiles in human milk serum and fat-globule membrane. | gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-mr glycoprotein bands. the properties of the components corresponding to the three bands (tentatively termed 'components c, b and a' in their order of migration) were compared by staining with four monoclonal antibodies and lectins. components b and c both reacted with the four antibodies, but component a did not. components b and c were stained with peanut (arachis hypogaea) agglutinin (pna) and wheat (triticum ... | 1986 | 3707520 |
| partial characterization of an allergenic glycoprotein from peanut (arachis hypogaea l.). | a concanavalin a-reactive glycoprotein allergen has been isolated from peanut (arachis hypogaea). the allergen was separated by affinity chromatography and purified by gel permeation and ion-exchange chromatography. the monomeric molecular weight is 65,000 and the pi is 4.6. the presence of one cysteine residue per molecule results in some dimer formation. concanavalin a-reactive glycoprotein is a potent allergen for peanut-sensitive patients in both in vivo and in vitro tests. it is allergenica ... | 1986 | 3708000 |
| cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms. | the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ... | 1986 | 3709546 |
| lectin binding sites on the surfaces of the pneumonocytes in human neonatal lung. | the surface coating of the pneumonocytes in human neonatal lung was studied by means of an electron microscope technique. slices of aldehyde-fixed lung tissue were labelled with a horseradish peroxidase conjugate of one of the following lectins: dolichos biflorus lectin, triticum vulgaris lectin, canavalia ensiformis lectin (concanavalin a), limulus polyphemus lectin, lotus tetragonolobus lectin and arachis hypogaea lectin. the tissue slices were then incubated in a diaminobenzidine-hydrogen per ... | 1986 | 3733468 |
| lectin-binding sites in human parathyroid tissue. | the aim of this study was to demonstrate several lectin-binding sites in human parathyroid tissue and to correlate these results with functional activity. the following lectins were tested for binding sites with certain carbohydrates (in parentheses): arachis hypogea (pna) (galactose), ulex europaeus i (uea) (fucose) and concanavalin a (cona) (mannose). in addition to normal parathyroids used as controls (13 cases), we examined adenomas associated with a clinical picture of primary hyperparathyr ... | 1986 | 3734421 |
| total substitution of leaf protein in the ration of laying hens. | laying birds of the harco strain (crossbred sex-linked brown leghorn and rhode island red) were fed rations in which leaf protein replaced 0, 25, 50, 75, and 100% of the soybean meal. these five rations were compared with a standard layer ration that contained fish meal and ground nut cake. the results show that 75% of the soybean meal in layer rations can be replaced by leaf protein without any significant effect in egg production and feed efficiency when compared with the performance of birds ... | 1986 | 3737531 |
| histochemistry of glycosaminoglycans in cartilage ground substance. alcian-blue staining and lectin-binding affinities in semithin epon sections. | the critical-electrolyte-concentration staining method using alcian blue (ab) was applied to etched semithin epon-embedded sections. the distribution of various glycosaminoglycans (gags) was studied in hyaline, elastic, cellular and fibrous cartilage obtained from humans and rodents. the staining patterns in semithin sections were found to correspond to those obtained using paraffin-embedded material. lectin histochemistry was performed on consecutive sections. the following peroxidase-labelled ... | 1986 | 3744895 |
| analysis of saccharide binding to artocarpus integrifolia lectin reveals specific recognition of t-antigen (beta-d-gal(1----3)d-galnac). | the binding of artocarpus integrifolia lectin to n-dansylgalactosamine (where dansyl is 5-dimethylaminonaphthalene-1-sulfonyl) leads to a 100% increase in dansyl fluorescence with a concomitant blue shift in the emission maximum by 10 nm. this binding is carbohydrate-specific and has an association constant of 1.74 x 10(4) m-1 at 20 degrees c. the lectin has two binding sites for n-dansylgalactosamine. the values of -delta h and -delta s for the binding of n-dansylgalactosamine are in the range ... | 1986 | 3745164 |
| changes in lectin binding patterns in the developing pulmonary vasculature of the pig lung. | lectin binding patterns to the developing pulmonary vasculature were studied in 10 large white pigs aged 1 min to 1 week and in three adult animals. paraffin-embedded tissue sections were exposed to eight lectin peroxidase conjugates: dolichos biflorus, triticum vulgaris, concanavalin a, ricinus communis type 2, arachis hypogaea, ricinus communis type 1, tetragonolobus purpureas and ulex europeus. lectin binding patterns to the pulmonary arterial and venous endothelium, to smooth muscle cells (s ... | 1986 | 3746525 |
| neurotoxicity effects of some agglutinins extracted from two edible legumes--lima bean (phaseolus lunatus) and groundnut (arachis hypogea)--in growing albino rats. | 1986 | 3751178 | |
| secretory glycoproteins of major vestibular glands (bartholin's glands) in female calves. studies with usual histochemical methods and with lectin-horseradish peroxidase conjugates in normal animals and in animals implanted with anabolic drugs. | the nature of mucins synthetized by the major vestibular glands of normal female calves and animals treated by anabolic drugs was investigated by usual histochemical methods and by lectin conjugate methods: canavalia ensiformis (con a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca i), glycine max (sba) and triticum vulgaris (wga). two main secretory cell types, i.e. acinar and tubular cells, could be distinguished. the former produced sialom ... | 1986 | 3751491 |
| crystallization of bowman-birk type protease inhibitor (peanut) and its complex with trypsin. | crystallization and preliminary crystallographic study of bowman-birk type protease inhibitors, a-i, a-ii, and b-iii from peanut seeds (arachis hypogaea), and of the a-ii + trypsin complex were carried out. a-ii, with 70 amino acid residues, crystallizes in a trigonal system, p3(1)21 (or p3(2)21), a = 71.8, c = 65.9 a, z = 12 or 18. the a-i crystal is isomorphous with that of a-ii, indicating that the n-terminal residues are in a disordered state in both crystals. the b-iii crystal is monoclinic ... | 1986 | 3759935 |
| triacylglycerol synthesis in developing seeds of groundnut (arachis hypogaea): lipid accumulation and incorporation of labeled precursors during seed maturation. | 1986 | 3770789 | |
| a simplified methodology for purification of peanut (arachis hypogaea) agglutinin. | the agglutinin from peanut (arachis hypogaea) was readily isolated by affinity chromatography on acid-treated sepharose 6b. the recovered lectin (50 mg/100 g seeds) appeared as a single band of mr 32,000 on gel electrophoresis and its specific haemagglutination titre on desialylated human a red blood cells was very high (2(15)). | 1986 | 3782717 |
| glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation. | expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. each lectin revealed a characteristic binding pattern during the phenotypic change of the nephrogenic mesenchyme and during distinct stages of nephron development. the uninduced mesenchymal cells were positive for pisum sativum (psa), concanavalin a (cona), wistaria floribunda (wga), and ricinus communis (rca-i) lectins. however, these lectins failed to react with t ... | 1987 | 3794308 |
| selective lectin reactions of two stocks of leishmania enriettii with differing pathogenicity. | five days old promastigote culture forms of two stocks of leishmania enriettii pathogenic and non-infective for cavia procellus, were tested with the lectins of canavalia ensiformis, ricinus communis-120, soja hispida (glycine maxima), arachis hypogaea, ulex europaeus, ulex europaeus i, ulex europaeus ii, laburnum alpinum, lotus tetragonolobus, euonymus europaeus and with a monoclonal antibody against blood group h. the pathogenic stock reacted with the anti-h lectin of lotus tetragonolobus and ... | 1987 | 3809146 |
| red cell th activation: biochemical studies. | the peanut agglutinin from arachis hypogea is a lectin that reacts with red blood cells expressing the th antigen. the th antigen has been said to be qualitatively similar to the t antigen, a well-defined antigen due to desialylation of glycophorin a and b that also reacts with the peanut agglutinin. we examined th activated red blood cells from two patients with fanconi's anaemia using 125i radiolabelled peanut agglutinin as a probe in western blotting of red blood cell membrane proteins. we al ... | 1987 | 3828228 |
| amino acid composition of alpha-amylase protein inhibitor from arachis hypogaea seeds. | 1985 | 3880495 | |
| an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat. | ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ... | 1985 | 3915936 |
| an evaluation of total and digestible lysine as a predictor of lysine availability in protein concentrates for young pigs. | forty pigs between 23 and 51 d of age were given, ad lib., diets containing 7.9 g lysine/kg. the diets contained wheat and one of five protein concentrates: milk, peanut (arachis hypogaea) meal, cotton-seed (gossypium herbaceum) meal, meat meal or lupins (lupinus augustifolius). twenty of the pigs were given an indigestible marker, 103ru-labelled tris-(1,10-phenanthroline)-ruthenium(ii) chloride, between 51 and 56 d of age and were killed at 56 d of age. a further forty pigs, between 23 and 51 d ... | 1985 | 3933552 |
| [development of two assay systems of desialylated hcg specific for o-serine or n-asparagine linked carbohydrate chain and their clinical application]. | two specific and sensitive assays have been developed for the measurement of as-hcg in this study. these assay systems are immunoradiometric assays which utilize peanut lectin, arachis hypogaea (pna) or caster bean lectin, ricin communis agglutinin (rca-i), to selectively extract as-hcg and then directly quantify with purified and radiolabeled antiserum against hcg beta cooh terminal peptide (beta-ctp). pna is highly specific for the terminal carbohydrate linkage gal beta-(1----3)-galnac which i ... | 1985 | 3934006 |
| flow cytometric analysis of heterogeneity in blood group-related antigen expression in a human urinary bladder carcinoma cell line, 647v. | previous immunohistological studies showed a relationship between expression of blood group-related antigens (bg-ag) and invasive potential in human urinary bladder carcinoma, but the marked variability of antigen staining within many individual tumors has obscured the biological basis of this finding. we studied the expression of the a, h, and t (thomsen-friedenreich) bg-ag by flow cytometry in a human bladder carcinoma cell line (647v) using fluoresceinated bg-ag-specific lectins (dolichos bif ... | 1985 | 4005841 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| lectin binding affinities of induced pancreatic lesions in the hamster model. | we examined the binding pattern of nine lectins to n-nitrosobis(2-oxopropyl)amine (bop)-induced pancreatic lesions in syrian hamsters. these lectins were arachis hypogaea (pna), dolichos biflorus (dba), griffonia simplicifolia i (gs-i), helix aspersa (haa), helix pomatia (hpa), sophora japonica (sja), ricinus communis i (rca-i), triticum vulgaris (wga) and ulex europaeus i (uea-i). all of the lectins reacted in untreated control hamsters to varying intensities with cytoplasmic components of acin ... | 1985 | 4064253 |
| glycoside-sensitive atpase from arachis hypogaea. | 1964 | 4220524 | |
| activity patterns of purified atpase from arachis hypogaea. | 1965 | 4221388 | |
| a study of free amino acids in rice moth larvae during mycotoxicosis. | free amino acid pattern has been studied in rice moth larvae during aflatoxicosis and compared with normal in ground nut meal and wheat bran. it was observed that there is an increase in free amino acids in intoxicated larvae and this was directly proportional to the degree of toxicity as indicated by decrease in growth of these larvae. the study of excretary pattern of amino acids shows that intoxicated larvae excrete less amino acids than the normal larvae. the results have been discussed. | 1968 | 4230279 |
| [on plant protease inhibitors. vi. purification of polyvalent protease inhibitors from arachis hypogaea]. | 1969 | 4241153 | |
| on the specificity of lectins with a broad agglutination spectrum. ii. studies on the nature of the t-antigen and the specific receptors for the lectin of arachis hypogoea (ground-nut). | 1969 | 4244395 | |
| the development of glyoxysomes in peanut cotyledons and maize scutella. | the development of glyoxysomes during germination has been studied in isolated peanut (arachis hypogaea l.) cotyledons and in maize (zea mays l.) scutella. in peanut cotyledons isocitratase, malate synthetase, and protein associated with the glyoxysomal fraction increase simultaneously from the 3rd to the 8th day of incubation. in scutella of germinating maize seeds the specific activities of isocitratase, malate synthetase, and catalase associated with the glyoxysomes rise until the 4th day of ... | 1970 | 4316376 |
| [sphingolipids and glycolipids of fungi and higher plants. v. on the structure of the phytoglycolipid from arachis hypogaea l]. | 2013 | 4390049 | |
| influence of some trace elements on the growth of rhizobium from peanut (arachis hypogaea l.) and gram (cicer arietinum l.). | 1972 | 4679121 | |
| derivation, characteristics and large scale culture of a cell line from arachis hypogaea, l. cotyledons. | 1971 | 4950438 | |
| [isolation from peanut seeds (arachis hypogaea l.) of an inhibitor of proteolytic enzymes]. | 1968 | 4971302 | |
| a basic trypsin- and chymotrypsin-inhibitor from groundnuts (arachis hypogaea). | 1972 | 5034214 | |
| [the isolation and characterisation of a lipase inhibitor from the lipids of arachis hypogaea]. | 1972 | 5052944 | |
| induction of giant cells in suspension cultures of arachis hypogaea, l. by massive irradiation. | 1971 | 5137461 | |
| occurrence of aflatoxin in ground nuts, some other nuts and industrial proteins imported into finland. | 1971 | 5147639 | |
| divisions in isolated cells of palisade parenchyma of arachis hypogaea. | 1965 | 5328671 | |
| endocarpic microorganisms of two types of windrow-dried peanut fruit (arachis hypogaea l.). | the endocarpic microorganisms of peanut fruit dried in either a random windrow (plants left as they fell from the digger) or an inverted windrow (plants inverted to expose fruit to sunlight) were different from that of freshly dug fruit. chaetomium, penicillium, trichoderma, rhizoctonia, and fusarium were the dominant fungi found associated with shells (pericarp) of freshly dug fruit. the dominant fungi of shells of windrowed fruit included chaetomium, rhizoctonia, fusarium, sclerotium, and alte ... | 1970 | 5466133 |
| characterization of thiamine in the raw peanut (arachis hypogaea l.). | 2015 | 5474252 | |
| purification and properties of a nonspecific hemagglutinin from the peanut (arachis hypogaea). | 1970 | 5495517 | |
| effects on fibrin clot lysis of a trypsin inhibitor in peanuts (arachis hypogaea). | 1967 | 5626247 | |
| growth of isolated palisade cells of arachis hypogaea in vitro. | 1968 | 5642026 | |
| beware the goober pea. | 1968 | 5678468 | |
| a comparative study of hypocholesterolemic activity of lipid extract of bengal gram with safflower oil, mustard oil and ground-nut oil in albino rats. | 1968 | 5711604 | |
| cytogenetics of x-ray induced aneuploids in arachis hypogaea l. | 1968 | 5717670 | |
| aflatoxin-producing potential of isolates of the aspergillus flavus-oryzae group from peanuts (arachis hypogaea). | seventy-eight samples of farmer stock peanuts, representing peanuts grown in nine different geographical areas during 1964, were assayed for aflatoxin and examined for associated microflora. only two samples contained more than 50 ppb of aflatoxin. infestation by members of the aspergillus flavus-oryzae group varied from 35 to 100% of the kernels per area and from 1 to 100% of the kernels per sample. aflatoxin production by individual isolates ranged from 0 to 349,143 ppb under the test conditio ... | 1967 | 6031427 |
| nutritive value of groundnut (arachis hypogaea). 1. amino acid composition of different varieties of groundnut grown in the punjab. | 1967 | 6052870 | |
| nutritive value of groundnut (arachis hypogaea). 2. biological evaluation of different varieties of groundnut grown in the punjab. | 1967 | 6052875 | |
| calcium-sodium interaction in the pod development of the peanut, arachis hypogaea l. | 1967 | 6065781 | |
| [catalytic concentration, multiple forms, and lectin affinity of microsomal enzymes from human tissues: lectins as reagents, ii (author's transl)]. | we estimated the concentrations, multiple forms, and lectin binding of five microsomal enzymes in particle free extracts from human kidney, pancreas, jejunal mucosa, and normal and cancerous liver. while arylesterase markedly reacted only with concanavalin a, arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase were intensely precipitated by lectins from ricinus communis 120, canavalia ensiformis, triticum vulgare and phaseolus vulgaris s. agglutinins from glycine max ... | 1981 | 6120206 |
| lectin-dependent attachment of actinomyces naeslundii to receptors on epithelial cells. | the adherence of actinomyces naeslundii wvu45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain wvu45. the attachment of actinomyces viscosus t14v, which has both types 1 and 2 fimbriae, was approximately half that of a. naeslundii, and only minimal attachment of a. naeslundii and a. viscosus mutants lacking type 2 fimbriae was detected. the adherence of strain wvu45 was enhanced two- to threefold by neuraminidase treatment of ... | 1984 | 6150008 |
| histochemical methods for the further characterisation of the tumourettes of the posterior lobe of the pituitary. | the granular cell tumourettes of the posterior lobe of the pituitary possess neuraminic acid containing carbohydrate. after removal of neuraminic acid with neuraminidase and exposure to fitc (fluorescein isothiocyanate) labelled peanut agglutinin (arachis hypogaea) (pna), intracellular receptor structures could be demonstrated. the significance of the findings is discussed. | 1980 | 6153492 |
| isolation, characterization and implications of anti-tf (thomsen-friedenreich) agglutinins from different sources. | anti-tf agglutinins from peanut (arachis hypogaea) and from vertebrate sera of different species have been successfully isolated by affinity chromatography on acid-activated sepharose 4 b. the proteins were characterized by immunoelectrophoresis, polyacrylamide gel electrophoresis in the presence of sds and with respect to their carbohydrate binding specificities. anti-tf substances from sera showed one precipitin arc in immunoelectrophoresis, but quantitative immunoprecipitation revealed our hu ... | 1980 | 6157637 |
| purification and properties of an alpha-amylase protein-inhibitor from arachis hypogaea seeds. | a protein showing highly specific inhibitory activity towards hog pancreatic and human salivary alpha-amylases (1,4-alpha-d-glucan glucanohydrolase, ec 3.2.1.1), but not towards plant and bacterial alpha-amylases, has been purified 197-fold from an aqueous extract of peanut cotyledons using heat treatment, (nh4)2so4 precipitation and ion-exchange chromatography on deae- and cm-cellulose. the purified inhibitor was homogeneous by polyacrylamide gel electrophoresis. its molecular weight, as determ ... | 1981 | 6167287 |
| cytochemical localization of small intestinal glycoconjugates by lectin histochemistry in controls and subjects with cystic fibrosis. | human mucosal glycoconjugates were examined in normal small intestinal biopsies from five control subjects using six different fluorescein-conjugated lectins: triticum vulgare agglutinin (wga), ulex europaeus agglutinin i (uea1), ricinus communis agglutinin i (rca1), glycin max-soy bean agglutinin (sba), dolichus biflorus agglutinin (dba), and arachis hypogaea peanut agglutinin (pna). these plant agglutinins bind to specific nonreducing end-terminal carbohydrate residues. only the lectins derive ... | 1983 | 6188589 |
| lectin binding patterns in diffuse large cell lymphoma. | the staining reaction of a panel of lectins in paraffin embedded lymph node specimens of diffuse large cell lymphoma was studied in relation to survival. in 47 of 49 patients, varying degrees of lectin binding were observed with ricinus communis agglutinin (rca), crude extract of arachis hypogaea (c-pna), concanavalin ensiformis a (con a), triticum vulgaris a (wga) and phaseolus vulgaris a (pha). binding was either absent or only minimal with pisum sativum a (psa) and lens culinaris a (lca). two ... | 1983 | 6194878 |
| lectin receptors as markers of lymphoid cells. ii. reed-sternberg cells share lectin-binding properties of monocyte macrophages. | the origin of the reed-sternberg cells of hodgkin's disease is controversial with proponents of transformed lymphocyte and macrophage histiocyte derivations. lectin receptors are potentially useful new cell markers in the investigation of lymphoproliferative disease. in this study, the lectin-binding properties of the reed-sternberg cell were investigated in an effort to clarify the cell of origin. utilizing a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, le ... | 1984 | 6206728 |
| peanut agglutinin (pna) binding sites: a useful marker for hormonal dependence in experimental breast cancer. | histochemical studies were performed with the labeled lectin from arachis hypogaea (peanut agglutinin, pna) to detect the occurrence, distribution, and amount of this lectin receptor in n-nitrosomethylurea-induced rat mammary carcinomas. since in normal rat mammary tissue, the formation and secretion of pna binding sites are controlled by the combined action of estrogen and prolactin, the amount of secretion-associated pna binding sites was correlated with tumor response to either ovariectomy or ... | 1983 | 6309384 |
| sheep peripheral blood-t-lymphocytes: isolation, separation and surface receptors for helix pomatia agglutinin and peanut agglutinin from arachis hypogaea. | 1984 | 6335945 | |
| differentiation of trypanosoma cruzi chagas, 1909 and trypanosoma vespertilionis battaglia, 1904 by various lectins. | four-days-old culture forms of trypanosoma cruzi (strain téhuantépéc, guatemala) and trypanosoma vespertilionis (strain p-14, p-9) were tested by 19 carbohydrate-specific agglutinins. the t. cruzi strains are interspecifically distinguishable with the lectins from euonymus europaeus, tridacna crocea, tridacna maxima and the human blood-group testserum anti-b from the t. vespertilionis strains. while the t. vespertilionis strains did react with anti-b and e. europaeus, the t. cruzi strains did no ... | 1983 | 6349060 |
| comparative studies of trypanosoma cruzi and t. cruzi-like stocks from different south american countries using lectins. | the agglutination behaviour of four-day-old epimastigote culture forms of 34 trypanosoma cruzi, and t. cruzi-like stocks from brazil, chile, colombia, ecuador and peru were tested with 15 carbohydrate-specific lectins. we distinguished intraspecifically two groups of agglutination reactions: group 1 includes stocks which react with triticum vulgaris and aaptos papillata ii (wheat germ agglutinin: wga-type). group 2 includes stocks agglutinated by arachis hypogaea and aaptos papillata ii (peanut ... | 1983 | 6362253 |
| intraspecific characterization of t. cruzi stocks by the lectins from triticum vulgaris and arachis hypogaea. | the agglutination behavior of four-days-old epimastigote culture forms of t. cruzi, and t. cruzi-like stocks from different south american countries were tested with 15 lectins. two agglutination types can be distinguished: typ 1 includes stocks which react with triticum vulgaris and aaptos papillata ii (wga-type). type 2 includes stocks agglutinated by arachis hypogaea and aaptos papillata ii (pna-type). the reactions with the lectins from triticum vulgaris and aaptos papillata ii can be correl ... | 1984 | 6375213 |
| lectin receptors as markers of lymphoid cells. i. demonstration in tissue section by peroxidase technique. | the distribution of lectin binding receptors in human lymphoid tissue (lymph node and tonsil) was studied in an effort to identify lectins with potential application as cell markers. using a simple peroxidase technique applicable to formalin-fixed, paraffin-embedded tissue, the binding properties of five lectins were investigated: concanavalin a (con a), arachis hypogaea (peanut agglutinin), lotus tetragonolobus (asparagus pea), glycine max (soybean agglutinin) and bandeiraea simplicifolia. thre ... | 1984 | 6380299 |
| differentiation between trypanosoma cruzi and trypanosoma rangeli on the basis of their sialic acid content. | four day-old culture forms of trypanosoma rangeli strain san augustin were tested with the following lectins: canavalia ensiformis, pisum sativum, ricinus communis-120, sojahispida, aaptos papillata ii, triticum vulgaris, limulus polyphemus, lberis amara and arachis hypogaea. these flagellates were only agglutinated by the lectins from canavalia ensiformis and pisum sativum. furthermore four day-old culture forms of the t. cruzi strains morcego 1354, p-60, téhuantépéc and wa 301/130 as well as t ... | 1984 | 6388083 |
| lectin--peroxidase conjugates in histopathology of gastrointestinal mucosa. | peroxidase (hrp) conjugates of dolichos biflorus agglutinin (dba), arachis hypogaea agglutinin (pna) and ulex europaeus agglutinin i (uea i) were used as specific molecular probes in order to describe histological patterns of carbohydrate moieties in glycoconjugates of gastric mucosa. specimens of normal mucosa, metaplasias and carcinomas were fixed, embedded in paraffin and stained with lectins by a postembedding technique. in normal mucosa, dba reacted with surface epithelial, parietal and ant ... | 1983 | 6402842 |
| [inhibitory effect of lectins extracted from arachis hypogea and soybeans on germ cell migration in the embryo of xenopus laevis (amphibia, anura)]. | effect of injection of arachis hypogea (pna) and soybean (sba) lectins on the migration of xenopus primordial germ cells (pgcs) were investigated. pna inhibited almost entirely the pgcs migration, more than sba. our results indicated that the inhibition of the migration could be a consequence of the lectin-induced alterations of cell surface properties. | 1983 | 6412978 |
| cellular localization of lectin-affinity in tissue sections of normal human duodenum. | peroxidase (hrp) conjugates of arachis hypogaea agglutinin (pna), dolichos biflorus agglutinin (dba), ulex europaeus agglutinin i (uea i) and bandeiraea simplicifolia agglutinin i (bsa i) were used to detect the expression of carbohydrate moieties in glyco-conjugates of normal human duodenal mucosa. lectin histology was performed on sections from paraffin embedded specimens of donor blood groups a, b, ab and 0. while pna, dba and uea i staining appeared to be independent of the donor blood group ... | 1983 | 6419452 |
| spatio-temporal patterns of differentiation of whole heads of the embryonic chick as revealed by binding of a fitc-coupled peanut-agglutinin (fitc-pna). | the spatio-temporal expression of specific binding sites for fitc-labeled peanut agglutinin (arachis hypogaea) in frozen sections of whole embryonic heads of the chick between embryonic day 2 (e2) and embryonic day 8 (e8) is described. pna-binding sites are present already at e2 on the external limiting membrane and the ventricular boundary of the neural wall. with progressing differentiation fluorescent bands are formed from the outside of the neural wall radiating inside, forming second layers ... | 1984 | 6423214 |
| purification and properties of a stilbene synthase from induced cell suspension cultures of peanut. | stilbene synthase ( resveratrol -forming) converts one molecule of rho- coumaroyl -coa and three molecules of malonyl-coa into 3,4',5- trihydroxystilbene . following selective induction of stilbene synthesis in cell suspension cultures of peanut (arachis hypogaea), the enzyme was extracted and purified to apparent homogeneity by chromatography on deae-cellulose and hydroxylapatite. the enzyme was found to be a dimer of estimated mr = 90,000 exhibiting under denaturing conditions a subunit mr of ... | 1984 | 6427224 |
| lectin binding sites of the mouse ovary, intraovarian and ovulated ova. | fluorescein isothiocyanate (fitc) labeled lectins were used to study the distribution of specific binding sites in histologic sections of mouse ovaries as well as ovulated ova. four distinct patterns of reactivity of the components of the follicle (exclusive of the ovum) and the surrounding ovarian stroma were recognized: uniform staining of granulosa cells, theca cells and surrounding stroma; weak to moderate staining of the granulosa cells and strong staining of the theca cells and stromal cel ... | 1984 | 6432745 |
| the role of the glycoconjugates in the migration of anuran amphibian germ cells. | the presence of a large amount of glycoconjugates on the anuran amphibian germ cells was demonstrated using fluorescein isothiocyanate lectins binding specifically to d-galactose and at a lower level, by other lectins binding specifically to n-acetyl-galactosamine. glycoconjugates including d-galactose were found near the pseudopodial expansions and in the extracellular space, between germ cells and follicular cells. they were also disseminated in the cytoplasm. the injection of pna lectin (from ... | 1984 | 6436429 |
| cona- and pna-binding glycoproteins of human epidermis. | concanavalia ensiformis agglutinin (cona) and arachis hypogaea agglutinin (pna) binding glycoproteins in np-40 extracts of human epidermis and epidermal cell preparations have been investigated by incubation of sds-polyacrylamide gels with 125i-labeled lectins. in whole epidermis, 125i-cona labels numerous glycoproteins, of which some appear to be restricted to the more differentiated upper layers of the epidermis and thus may represent markers of epidermal differentiation. these glycoproteins a ... | 1984 | 6470525 |
| lectins as probes in histochemistry and immunohistochemistry: the peanut (arachis hypogaea) lectin. | 1984 | 6479971 | |
| a possible mechanism for the cellular coaggregation between actinomyces viscosus atcc 19246 and streptococcus sanguis atcc 10557. | the cells of actinomyces viscosus atcc 19246 (av19246) and streptococcus sanguis atcc 10557 (ss10557) coaggregated immediately after mixing in 40 mm-tris/hcl buffer. optimal conditions were ph 7.5 in the presence of ca2+ at 0.1 mm or higher. na2 edta and its analogues, na2mgedta and na2mnedta at 7.5 mm inhibited the coaggregation. trypsin and heat treatment impaired the reactive site on av19246 cells, but not on ss10557 cells. the coaggregates, once formed, dissociated gradually during extended ... | 1984 | 6481336 |