Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| changes in the levels of polymethoxyflavones and flavanones as part of the defense mechanism of citrus sinensis (cv. valencia late) fruits against phytophthora citrophthora. | phytophthora citrophthora causes serious losses in citrus fruits through brown rot lesion. the effect of infection with p. citrophthora on citrus sinensis (cv. valencia late) fruits was studied, with particular reference to the levels of the flavanones hesperidin and isonaringin and the polymethoxyflavones sinensetin, nobiletin, tangeretin, and heptamethoxyflavone, because flavonoids are most probably involved as natural defense or resistance mechanisms in this genus. changes in the levels of th ... | 2004 | 15053528 |
| potent inhibition by star fruit of human cytochrome p450 3a (cyp3a) activity. | there has been very limited information on the capacities of tropical fruits to inhibit human cytochrome p450 3a (cyp3a) activity. thus, the inhibitory effects of tropical fruits on midazolam 1'-hydroxylase activity of cyp3a in human liver microsomes were evaluated. eight tropical fruits such as common papaw, dragon fruit, kiwi fruit, mango, passion fruit, pomegranate, rambutan, and star fruit were tested. we also examined the inhibition of cyp3a activity by grapefruit (white) and valencia orang ... | 2004 | 15155547 |
| interaction between endophytic bacteria from citrus plants and the phytopathogenic bacteria xylella fastidiosa, causal agent of citrus-variegated chlorosis. | to isolate endophytic bacteria and xylella fastidiosa and also to evaluate whether the bacterial endophyte community contributes to citrus-variegated chlorosis (cvc) status in sweet orange (citrus sinensis [l.] osbeck cv. pera). | 2004 | 15189288 |
| a beta-galactosidase gene is expressed during mature fruit abscission of 'valencia' orange (citrus sinensis). | beta-galactosidases have been detected in a wide range of plants and are characterized by their ability to hydrolyse terminal non-reducing beta-d-galactosyl residues from beta-d-galactosides. these enzymes have been detected in a wide range of plant organs and tissues. in a search for differentially expressed genes during the abscission process in citrus, sequences encoding beta-galactosidase were identified. three cdna fragments of a beta-galactosidase gene were isolated from a cdna subtraction ... | 2004 | 15208347 |
| a new virus related to satsuma dwarf virus: the nucleotide sequence of the 3'-terminal regions of hyuganatsu virus rnas 1 and 2. brief report. | the sequences of the 3'-terminal 1306 and 2160 nucleotides of rnas 1 and 2 of a virus serologically related to satsuma dwarf virus (sdv) from hyuganatsu ( citrus tamurana hort. ex tan.) were determined, respectively. we found that the partial rna-dependent rna polymerase region in rna1 and the coat proteins (cps) region in rna2 of the virus tentatively named hyuganatsu virus (hv) have 78.3-84.0% and 76.9-80.7% amino acid sequence identities to those of known sdv-related viruses (sdv-rvs), i.e., ... | 2004 | 15221545 |
| nitrate improves growth in salt-stressed citrus seedlings through effects on photosynthetic activity and chloride accumulation. | we analyzed the effects of nitrate availability on growth of navelina (citrus sinensis (l.) osbeck) scions grafted on three citrus rootstocks differing in salt tolerance: carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.), citrus macrophylla wester and cleopatra mandarin (citrus reshni hort. ex tanaka). salt stress reduced total plant biomass by 27-38%, whereas potassium nitrate supplementation partially counteracted this effect by increasing dry matter and new leaf a ... | 2004 | 15234900 |
| isolation and characterization of a terminal flower homolog and its correlation with juvenility in citrus. | terminal flower is a key regulator of floral timing in arabidopsis and other herbaceous species. a homolog of this gene, cstfl, was isolated from the hybrid perennial tree crop washington navel orange (citrus sinensis l. osbeck). the deduced amino acid sequence of cstfl was 65% identical to the arabidopsis tfl1 protein. wild-type arabidopsis plants ectopically expressing cstfl showed late-flowering phenotypes similar to those described for overexpression of arabidopsis tfl1. in addition, the 35s ... | 2004 | 15235113 |
| the salt-stress signal transduction pathway that activates the gpx1 promoter is mediated by intracellular h2o2, different from the pathway induced by extracellular h2o2. | several genes encoding putative glutathione peroxidase have been isolated from a variety of plants, all of which show the highest homology to the phospholipid hydroperoxide isoform. several observations suggest that the proteins are involved in biotic and abiotic stress responses. previous studies on the regulation of gpx1, the citrus sinensis gene encoding phospholipid hydroperoxide isoform, led to the conclusion that salt-induced expression of gpx1 transcript and its encoded protein is mediate ... | 2004 | 15247370 |
| introduction of a citrus blight-associated gene into carrizo citrange [citrus sinensis (l.) osbc. x poncirus trifoliata (l.) raf.] by agrobacterium-mediated transformation. | the protein p12 accumulates in leaves of trees with citrus blight (cb), a serious decline of unknown cause. the function of p12 is not known, but sequence analysis indicates it may be related to expansins. in studies to determine the function of p12, sense and antisense constructs were used to make transgenic carrizo citrange using an agrobacterium-mediated transformation system. homogeneous beta-glucuronidase+ (gus+) sense and antisense transgenic shoots were regenerated using kanamycin as a se ... | 2004 | 15248084 |
| interstock-induced mechanism of increased growth and salt resistance of orange (citrus sinensis) trees. | interstocks improve the growth and salt resistance of lemon (citrus limon (l.) burm. f.) trees, but their effects on orange (citrus sinensis (l.) osbeck) trees are unknown. we grew 'cleopatra' mandarin (cm) seedlings, budded trees of 'salustiano' orange (sao) on cm, 'valencia late' orange (vlo) on cm (vlo/cm), and interstock trees vlo/sao/cm in pots of sand watered with nutrient solution containing 5 (control) or 50 mm nacl for 12 weeks. plants were harvested on six successive occasions and the ... | 2004 | 15294757 |
| genetic transformation of mature citrus plants. | most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. evaluation of characteristics related to fruits is a requisite to release any new variety into the market. because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. direct transformation of mature material could en ... | 2005 | 15310921 |
| expression of green fluorescent protein in xylella fastidiosa is affected by passage through host plants. | xylella fastidiosa, a gram-negative bacterial plant pathogen, causes pierce's disease of grapevine in north america. in south america the pathogen causes citrus variegated chlorosis, which is widespread in brazil. we have introduced into xylella fastidiosa a mini-tn5 transposon that encodes a green fluorescent protein (gfp) gene optimized for expression in bacteria. the mini-tn5 derivative was inserted into different sites of the genome in independent transconjugants as determined by southern bl ... | 2004 | 15386107 |
| impact of genetically modified enterobacter cloacae on indigenous endophytic community of citrus sinensis seedlings. | enterobacter cloacae (strain pr2/7), a genetically modified endophyte (gme) in citrus plants, carrying different plasmids (pec3.0/18, pcele, pegla and pgfp), was inoculated into citrus sinensis seedlings under greenhouse conditions. the impact of this on the indigenous bacterial endophytic community was studied by analyses of 2 different morphologic groups. the germination rates of inoculated seeds were evaluated in greenhouse, and plasmid stability under in vitro conditions. results demonstrate ... | 2004 | 15459643 |
| a new potato virus in a new lineage of picorna-like viruses. | on constructing a cdna library for potato, 'contaminating' sequences with a significant identity to apple latent spherical virus (alsv) were found. determination of the remaining genome sequence indicated the presence of a bipartite virus with an rna1 and 2 of 7034 and 3315 nucleotides, respectively, excluding a poly(a)tail. rna1 encodes a single polyprotein (233 kda) and shares highest amino acid identity with alsv at 65%. conserved amino acid motifs typical for helicase, protease and rna-depen ... | 2004 | 15503203 |
| biochemical and molecular analysis of carotenoid biosynthesis in flavedo of orange (citrus sinensis l.) during fruit development and maturation. | changes in carotenoid content and composition and expression of carotenoid biosynthetic genes were analyzed in the flavedo of sweet orange (citrus sinensis l. osbeck, cv. navelate) fruit during development and maturation. lutein and all-e-violaxanthin were the major carotenoids in chloroplast-containing tissues. during fruit coloration, phytoene, beta-cryptoxanthin, zeaxanthin, and mainly (9z)-violaxanthin progressively accumulated, and a large proportion of apocarotenoids was also found in the ... | 2004 | 15506808 |
| analysis of antioxidant compounds in sweet orange peel by hplc-diode array detection-electrospray ionization mass spectrometry. | hplc-diode array detection-electrospray ionization mass spectrometry was used to determine qualitatively and quantitatively the flavonoid content of several fractions and residues of extracts of greek navel sweet orange peel (citrus sinensis) from the region of southern greece (leonidi-tripoli). the main groups of flavonoids found according to hplc retention times, spectral data and literature references were polymethoxylated flavones, c-glycosylated flavones, o-glycosylated flavones, o-glycosyl ... | 2005 | 15515108 |
| characterization of anthocyanins and pyranoanthocyanins from blood orange [citrus sinensis (l.) osbeck] juice. | anthocyanins from blood orange [citrus sinensis (l.) osbeck] juices were isolated and purified by means of high-speed countercurrent chromatography and preparative high-performance liquid chromatography. structures of the pigments were then elucidated by electrospray ionization multiple mass spectrometry and nuclear magnetic resonance (nmr) spectroscopy. the major anthocyanins of the juice were characterized as cyanidin 3-glucoside and cyanidin 3-(6"-malonylglucoside). furthermore, six minor ant ... | 2004 | 15563216 |
| cover crops and natural vegetation mulch effect achieved by mechanical management with lateral rotary mower in weed population dynamics in citrus. | the aim of this study was to obtain information on practical weed management in order to reduce the use of herbicides thereby contributing to the sustainable development of citrus crop. the experiment was carried out under field conditions at the experimental area of the department of vegetal production at the college of agriculture "luiz de queiroz," piracicaba, sp, brazil, during the season 2002-2003. influence of mulches produced by four types of vegetations on the dynamic population of weeds ... | 2005 | 15656179 |
| identification and aroma impact of norisoprenoids in orange juice. | four norisoprenoids, alpha-ionone, beta-ionone, beta-cyclocitral, and beta-damascenone, along with their putative carotenoid precursors, were identified in valencia orange juice using time-intensity gc-o, gc-ms, and photodiode array hplc. alpha-ionone and beta-cyclocitral are reported in orange juice for the first time. gc-o aroma peaks were categorized into seven groups with similar sensory qualities: citrus/minty, metallic/mushroom/geranium, roasted/cooked/meaty/spice, fatty/soapy/green, sulfu ... | 2005 | 15656678 |
| repellency and toxicity of aromatic plant extracts against the mosquito culex pipiens molestus (diptera: culicidae). | the insecticidal activities of essential oil extracts from leaves, flowers and roots of aromatic plants against fourth-instar larvae of the mosquito culex pipiens molestus forskal were determined. extracts of foeniculum vulgare mill were the most toxic, followed by those of ferula hermonis boiss, citrus sinensis osbeck, pinus pinea l, laurus nobilis l and eucalyptus spp with lc50 values of 24.5, 44.0, 60.0, 75.0, 117.0 and 120.0 mg litre(-1), respectively. combination tests between the lc50 and ... | 2005 | 15662650 |
| effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism. | the effect of nigerian citrus (citrus sinensis osbeck) honey on ethanol metabolism was tested using 45 consenting individuals in apparent good health and between the ages of 25 and 35 years. the subjects were moderate social drinkers matched in terms of body weight and build. the results obtained showed that on average, honey significantly (p < 0.05) increased the blood ethanol clearance rate by 68% and decreased the intoxication period by 43%, but insignificantly (p > 0.05) reduced the degree o ... | 2004 | 15662998 |
| inotropic effect of citrus sinensis (l.) osbeck leaf extracts on the guinea pig atrium. | the objective of the present investigation was to determine the contractile effect of crude and acetone leaf extracts of citrus sinensis (l.) osb. on mammalian myocardium. crude leaf extracts have been used in folk medicine to treat neurological disorders. some flavonoids isolated from this plant presented a positive inotropic effect on myocardium. this motivated us to test the extracts on the atria of guinea pigs of both sexes (300-500 g) and surprisingly we observed inotropic depression instea ... | 2005 | 15665997 |
| metal-binding proteins scanning and determination by combining gel electrophoresis, synchrotron radiation x-ray fluorescence and atomic spectrometry. | in the present work, protein bands from in vitro embriogenic callus (citrus sinensis l. osbeck) were investigated using micro-synchrotron radiation x-ray fluorescence (musr-xrf) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) separation. metal-binding protein quantification was done after microwave oven decomposition of gel by synchrotron radiation total reflection x-ray fluorescence (sr-txrf), flame atomic absorption spectrometry (faas) and flame atomic emission spect ... | 2005 | 15680280 |
| starter substrate specificities of wild-type and mutant polyketide synthases from rutaceae. | chalcone synthases (chss) and acridone synthases (acss) belong to the superfamily of type iii polyketide synthases (pkss) and condense the starter substrate 4-coumaroyl-coa or n-methylanthraniloyl-coa with three malonyl-coas to produce flavonoids and acridone alkaloids, respectively. acss which have been cloned exclusively from ruta graveolens share about 75-85% polypeptide sequence homology with chss from other plant families, while 90% similarity was observed with chss from rutaceae, i.e., r. ... | 2005 | 15680984 |
| establishment of a genetically marked insect-derived symbiont in multiple host plants. | alcaligenes xylosoxidans subsp. denitrificans, originally isolated from the cibarial region of the foregut of the glassy-winged sharpshooter (homalodisca coagulata), was transformed using the himar1 transposition system to express egfp. seedlings of six potential host plants were inoculated with transformed bacteria and 2 weeks later samples were taken 5 cm away and analyzed by quantitative real-time pcr using primers designed to amplify the gene insert. the largest colony of 3,591,427 cells/2 c ... | 2005 | 15723145 |
| the climacteric-like behaviour of young, mature and wounded citrus leaves. | although leaves and other vegetative tissues are generally considered as non-climacteric, citrus leaves show a climacteric system ii behaviour after detachment. upon harvest, young, fully expanded 'valencia' orange (citrus sinensis) leaves ( approximately 60-d-old) exhibited two phases of ethylene production. the first phase, up to 6 d after detachment, was characterized by a low and constant ethylene production (system i pathway), associated with a constitutive expression of acc synthase 2 (csa ... | 2005 | 15767320 |
| varietal and interspecific influence on micronutrient contents in citrus from the mediterranean area. | to specify the genotypic variation of mediterranean citrus juices, the contents of carotenoids, flavonoids, and vitamin c were determined by high-performance liquid chromatography. a selection of orange varieties and mandarin species from the mediterranean area (citrus sinensis, citrus deliciosa ten, and citrus clementina hort. ex tan) was evaluated using carotenoid profiles and flavanones contents. among the eight varieties of orange (salustiana, hamlin, shamouti, pera, valencia, maltaise, sang ... | 2005 | 15769147 |
| leaf volatile compounds of six citrus somatic allotetraploid hybrids originating from various combinations of lime, lemon, citron, sweet orange, and grapefruit. | volatile compounds were extracted by a pentane/ether (1:1) mixture from the leaves of six citrus somatic allotetraploid hybrids resulting from various combinations of lime, lemon, citron, sweet orange, and grapefruit. extracts were examined by gas chromatography-mass spectrometry (gc-ms) and compared with those of their respective parents. all hybrids having an acid citrus parent exhibit the same relative contents in hydrocarbons and oxygenated compounds as the acid citrus, while the (grapefruit ... | 2005 | 15769160 |
| isolation, characterization, and pectin-modifying properties of a thermally tolerant pectin methylesterase from citrus sinensis var. valencia. | the thermally tolerant pectin methylesterase (tt-pme) was isolated as a monocomponent enzyme from sweet orange fruit (citrus sinensis var. valencia). it was also isolated from flower and vegetative tissue. the apparent molecular weight of fruit tt-pme was 40800 by sds-page and the isoelectric point estimated as pi 9.31 by ief-page. maldi-tof ms identified no tryptic-peptide ions from tt-pme characteristic of previously described citrus pmes. tt-pme did not absolutely require supplemented salt fo ... | 2005 | 15769165 |
| citrus huanglongbing in são paulo state, brazil: pcr detection of the 'candidatus' liberibacter species associated with the disease. | symptoms of huanglongbing (hlb), one of the most serious diseases of citrus in asia and africa, have been noticed in march 2004 in the araraquara region of são paulo state, brazil. hlb has not been reported previously from america. the causal hlb bacteria, candidatus liberibacter africanus in africa and candidatus liberibacter asiaticus in asia, can be detected in symptomatic citrus leaves by pcr amplification of their 16s rdna with previously described primers. when this technique was applied t ... | 2005 | 15797817 |
| transcription analysis of pils and xpsel genes from xylella fastidiosa. | xylella fastidiosa is a xylem-limited phytopathogen responsible for diseases in several plants such as citrus and coffee. analysis of the bacterial genome revealed some putative pathogenicity-related genes that could help to elucidate the molecular mechanisms of plant-pathogen interactions. in the present work, the transcription of three genes of the bacterium, grown in defined and rich media and also in media containing host plant extracts (sweet orange, 'ponkan' and coffee) was analyzed by rt- ... | 2005 | 15803391 |
| a set of primers for analyzing chloroplast dna diversity in citrus and related genera. | chloroplast simple sequence repeat (cpssr) markers in citrus were developed and used to analyze chloroplast diversity of citrus and closely related genera. fourteen cpssr primer pairs from the chloroplast genomes of tobacco (nicotiana tabacum l.) and arabidopsis were found useful for analyzing the citrus chloroplast genome (cpdna) and recoded with the prefix spcc (ssr primers for citrus chloroplast). eleven of the 14 primer pairs revealed some degree of polymorphism among 34 genotypes of citrus, ... | 2005 | 15805086 |
| relationships between nutrient composition of flowers and fruit quality in orange trees grown in calcareous soil. | to determine if flower nutrient composition can be used to predict fruit quality, a field experiment was conducted over three seasons (1996-1999) in a commercial orange orchard (citrus sinensis (l.) osbeck cv. 'valencia late', budded on troyer citrange rootstock) established on a calcareous soil in southern portugal. flowers were collected from 20 trees during full bloom in april and their nutrient composition determined, and fruits were harvested the following march and their quality evaluated. ... | 2005 | 15805096 |
| effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant staphylococcus aureus. | ethanolic extracts and some fractions from 10 indian medicinal plants, known for antibacterial activity, were investigated for their ability to inhibit clinical isolates of beta-lactamase producing methicillin-resistant staphylococcus aureus (mrsa) and methicillin-sensitive s. aureus (mssa). synergistic interaction of plant extracts with certain antibiotics was also evaluated. the mrsa test strains were found to be multi-drug resistant and also exhibited high level of resistance to common beta-l ... | 2005 | 15812867 |
| protoplast transformation and regeneration of transgenic valencia sweet orange plants containing a juice quality-related pectin methylesterase gene. | valencia orange [citrus sinensis (l.) osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (tspme) reduces its juice quality. a long-term strategy of this work is to eliminate or greatly reduce tspme activity in valencia orange. previous work resulted in the isolation of a putative tspme gene, cspme4, associated with a thermostable protein fraction of valencia orange juice. to begin research design ... | 2005 | 15875190 |
| toward validation of process criteria for high-pressure processing of orange juice with predictive models. | mathematical models were developed to predict time to inactivation (tti) by high-pressure processing of salmonella in australian valencia orange juice (ph 4.3) and navel orange juice (ph 3.7) as a function of pressure magnitude (300 to 600 mpa) and inoculum level (3 to 7 log cfu/ml). for each model, the tti was found to increase with increasing inoculum level and decrease with increasing pressure magnitude. the u.s. food and drug administration juice hazard analysis and critical control point re ... | 2005 | 15895726 |
| g-protein-coupled alpha2a-adrenoreceptor agonists differentially alter citrus leaf and fruit abscission by affecting expression of acc synthase and acc oxidase. | temporal and spatial expression patterns of genes encoding 1-aminocyclopropane-1-carboxylate (acc) synthase (acs1 and acs2) and acc oxidase (aco), acc concentration, and ethylene production in leaves and fruit of 'valencia' orange (citrus sinensis [l.] osbeck) were examined in relation to differential abscission after treatment with 2-chloroethylphosphonic acid (ethephon) alone or in combination with guanfacine or clonidine, two g-protein-coupled alpha(2a)-adrenoreceptor selective agonists. guan ... | 2005 | 15928018 |
| antioxidant activity of the flavonoid hesperidin in chemical and biological systems. | the antioxidant hesperidin, a major flavonoid in sweet orange and lemon, was evaluated using chemical and biological systems. the chemical assay evaluates the hesperidin capacity to sequester 1,1-diphenyl-2-picrylhydrazyl (dpph*). biological studies were done using the eukaryotic cells of superoxide-dismutase proficient and deficient strains of saccharomyces cerevisiae treated with hesperidin and the stressing agents hydrogen peroxide or paraquat (methylviologen; 1,1'-dimethyl-4,4'-bipyridinium ... | 2005 | 15941311 |
| [changes in polyamine levels in citrus sinensis osb. cv. valencia callus during somatic embryogenesis]. | somatic embryogenetic capability and changes in polyamine level and their relationship were analyzed using the long-term (8 years) subcultured calli of citrus sinensis osb. cv. valencia as materials. the results showed that endogenous polyamine contents in embryogenic calli were higher than those in non-embryogenic calli, and the embryogenetic capability was positively correlated to the levels of endogenous polyamines. when the calli were transferred to a differentiation medium, the putrescine c ... | 2005 | 15961902 |
| involvement of ethylene biosynthesis and perception in the susceptibility of citrus fruits to penicillium digitatum infection and the accumulation of defence-related mrnas. | citrus fruits infected with the fungus penicillium digitatum substantially increase the production of the plant hormone ethylene. in this study, the regulation of ethylene biosynthesis in citrus sinensis-infected fruits and its putative involvement in an active defence response against p. digitatum infection is examined. ethylene production is demonstrated as being the result of the co-ordinated and differential up-regulation of at least three ethylene biosynthetic genes: acs1, acs2, and aco. bl ... | 2005 | 15983011 |
| nobiletin: efficient and large quantity isolation from orange peel extract. | it is known that nobiletin possesses anticancer, antiviral and anti-inflammatory activities. recently, the demand for nobiletin in large quantities and high purity has increased. however, conventional normal-phase silica gel chromatography and c(18)-reverse-phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. in exploring the composition and the biological activities of polymethoxyflavones from sweet orange (citrus sinensis) peel, we develo ... | 2006 | 15999338 |
| [control effects of mikania micrantha alcohol extract on citrus red mite panonychus citri]. | this study showed that spraying 0.2 and 0.4 g x l(-1) mikania micrantha alcohol extracts on panonychus citri could significantly decrease the survival rates of p. citri eggs, larvae and nymphs, and 0.1 g x l(-1) m. micrantha alcohol extract could also significantly decrease the survival rates of larvae and nymphs. in a no-choice test, both the fecundity and the longevity of female p. citri fed on the leaves treated with all three test concentrations 0.1, 0.2 and 0.4 g x l(-1) of m. micrantha alc ... | 2005 | 16011182 |
| analysis of flavanone-7-o-glycosides in citrus juices by short-end capillary electrochromatography. | the separation of the major flavanone-7-o-glycoside constituents of citrus was carried out by isocratic reversed phase capillary electrochromatography using a 75 microm i.d. silica fused column packed with 5 microm ods silica gel. in comparison to hplc mode, capillary electrochromatography resolution of flavanone glycosides was obtained with a high selectivity factor. optimum separation conditions were found using a mixture of ammonium formate (ph 2.5)--acetonitrile (8:2, v/v) as the mobile phas ... | 2005 | 16013605 |
| [creation and hybrid identification of the sexual progenies using citrus somatic hybrid as pollen parent]. | 110 plantlets were obtained from the cross between citrus allotetraploid somatic hybrid [hamlin sweet orange (citrus sinensis osback) + rough lemon (c. jambhiri luss)] (as the pollen parent) and mono-embryonic diploid type iben no.4 [huanongbendizao tangerine (c. reticulata blanco) x ichang papeda (c. ichangensis swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. rapd analyses sh ... | 2005 | 16044915 |
| lycopene-epsilon-cyclase pre-mrna is alternatively spliced in cara cara navel orange (citrus sinensis osbeck). | lycopene-epsilon-cyclase is one of the key enzymes related to alpha-carotene metabolism in plants. a full-length cdna of 1300 bp encoding lycopene-epsilon-cyclase (lyce) was generated from cara cara navel orange, a unique navel orange containing both lycopene and beta-carotene in its pulp, with little or no alpha-carotene. the gene had a 14 bp nucleotides deletion and caused a terminal mutation. dna sequence corresponding to the deletion region revealed that two repeats of 6 bp (aggtgt) were fla ... | 2005 | 16086259 |
| soil water repellency induced by long-term irrigation with treated sewage effluent. | this study describes soil water repellency developed under prolonged irrigation with treated sewage effluent in a semiarid environment. soil surface layer (0-5 cm) and soil profile (0-50 cm) transects were sampled at a high resolution at the close of the irrigation season and rainy winter season. samples from 0- to 5-cm transects were subdivided into 1-cm slices to obtain fine scale resolution of repellency and organic matter distribution. extreme to severe soil water repellency in the 0- to 5-c ... | 2005 | 16151242 |
| biological activity of carotenoids in red paprika, valencia orange and golden delicious apple. | carotenoid fractions were extracted from red paprika, valencia orange peel and the peel of golden delicious apple. thus, hypophasic carotenoids of paprika (pm1), orange (pm3) and apple (pm4), and epiphasic extractions of paprika (pm2) and apple (pm5) were obtained by extraction, saponification and partition between meoh-h(2)o (9:1) (hypophasic) and hexane (epiphasic). a high content of capsanthin was quantified in hypophasic carotenoids (pm1) from red spice paprika, whereas the hypophasic fracti ... | 2005 | 16177974 |
| action pattern of valencia orange pme de-esterification of high methoxyl pectin and characterization of modified pectins. | valencia pectinmethylesterase (pme) fractions, b-pme, containing 36 and 13 kda protein bands and u-pme, containing a 36 and 27 kda protein bands, were used to de-esterify original pectin (o-pec) from 73% degree of esterification (%de) to 63% (b-pec) and 61% de (u-pec), respectively. most o-pec eluted from ion exchange chromatography at low salt concentration and a smaller component eluted at higher ionic strength. b-pec and u-pec eluted as one broad peak at higher ionic strength. pme modificatio ... | 2005 | 16216228 |
| rapid, specific and quantitative assays for the detection of the endophytic bacterium methylobacterium mesophilicum in plants. | xylella fastidiosa is a xylem-limited bacterium that causes citrus variegated chlorosis disease in sweet orange. there is evidence that x. fastidiosa interacts with endophytic bacteria present in the xylem of sweet orange, and that these interactions, particularly with methylobacterium mesophilicum, may affect disease progress. however, these interactions cannot be evaluated in detail until efficient methods for detection and enumeration of these bacteria in planta are developed. we have previou ... | 2006 | 16266765 |
| biotransformation of citrus aromatics nootkatone and valencene by microorganisms. | biotransformations of the sesquiterpene ketone nootkatone from the crude drug alpiniae fructus and grapefruit oil, and the sesquiterpene hydrocarbon valencene from valencia orange oil were carried out with microorganisms such as aspergillus niger, botryosphaeria dothidea, and fusarium culmorum to afford structurally interesting metabolites. their stereostructures were established by a combination of high-resolution nmr spectral and x-ray crystallographic analysis and chemical reaction. metabolic ... | 2005 | 16272725 |
| highly efficient production of nootkatone, the grapefruit aroma from valencene, by biotransformation. | nootkatone, the most important and expensive aromatic of grapefruit, decreases the somatic fat ratio, and thus its demand is increasing in the cosmetic and fiber sectors. a sesquiterpene hydrocarbon, (+)-valencene, which is cheaply obtained from valencia orange, was biotransformed by the green algae chlorella species and fungi such as mucor species, botryosphaeria dothidea, and botryodiplodia theobromae to afford nootkatone in high yield. | 2005 | 16272746 |
| anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. | the aim of this work was to study the impact of moderately long storage periods at 4 degrees c upon red orange [citrus sinensis (l.) osbeck] anthocyanins production and the expression of structural genes involved in their biosynthesis such as phehylalanine ammonia lyase (pal), chalcone synthase (chs), dihydroflavonol 4-reductase (dfr), and udp-glucose flavonoid glucosyl transferase (ufgt). our results showed that low temperature-induced anthocyanins accumulation in red orange juice vesicles afte ... | 2005 | 16277406 |
| effects of ozone and sulfur dioxide on phyllosphere fungi from three tree species. | short-term effects of ozone (o(3)) on phyllosphere fungi were studied by examining fungal populations from leaves of giant sequoia (sequoiadendron giganteum (lindl.) buchholz) and california black oak (quercus kelloggii newb.). chronic effects of both o(3) and sulfur dioxide (so(2)) were studied by isolating fungi from leaves of mature valencia orange (citrus sinensis l.) trees. in this chronic-exposure experiment, mature orange trees were fumigated in open-top chambers at the university of cali ... | 1989 | 16347849 |
| a rapid liquid chromatography electrospray ionization mass spectrometry(n) method for evaluation of synephrine in citrus aurantium l. samples. | immature bitter orange fruit and its extracts have been introduced into the market as an alternative to ephedra in weight loss products. however, the safety of the immature bitter orange fruit and its extracts is a debated argument due to the presence of synephrine, a constituent known as a sympathomimetic agent. in this paper, we describe the development of a new, rapid, and simple liquid chromatography-electrospray ionization-tandem mass spectrometry method devoted to the quantitative determin ... | 2005 | 16366666 |
| cloning and characterization of two 9-cis-epoxycarotenoid dioxygenase genes, differentially regulated during fruit maturation and under stress conditions, from orange (citrus sinensis l. osbeck). | there is now biochemical and genetic evidence that oxidative cleavage of cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (nced) is the critical step in the regulation of abscisic acid (aba) synthesis in higher plants. the peel of citrus fruit accumulates large amounts of aba during maturation. to understand the regulation of aba biosynthesis in citrus, two full-length cdnas (csnced1 and csnced2) encoding nceds were isolated and characterized from the epicarp of orange fruits (citrus si ... | 2006 | 16396998 |
| quantitative real-time pcr for detection and identification of candidatus liberibacter species associated with citrus huanglongbing. | citrus huanglongbing (hlb, ex greening) is one of the most serious diseases of citrus. different forms of the disease are caused by different candidatus liberobacter species, candidatus liberibacter asiaticus (las), ca. l. africanus (laf) and ca. l. americanus (lam). the pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. the vector psyllid diaphorina citri can transmit both las and lam. establishment of this vector into florida, reports of lam and las in ... | 2006 | 16414133 |
| liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance as complementary analytical techniques for unambiguous identification of polymethoxylated flavones in residues from molecular distillation of orange peel oils (citrus sinensis). | liquid chromatography/mass spectrometry and liquid chromatography/nuclear magnetic resonance techniques with ultraviolet/diode array detection were used as complementary analytical tools for the reliable identification of polymethoxylated flavones in residues from molecular distillation of cold-pressed peel oils of citrus sinensis. after development of a liquid chromatographic separation procedure, the presence of several polymethoxy flavones such as sinensetin, nobiletin, tangeretin, quercetoge ... | 2006 | 16417279 |
| vacuolar citrate/h+ symporter of citrus juice cells. | we have isolated a cdna, designated citrus sinensis citrate transporter 1 cscit1 encoding a novel vacuolar citrate/symporter. immunoblots using antibodies raised against cscit1 showed that the protein is localized to the juice sac cell vacuoles. the highest expression of cscit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar ph. vacuoles from sacharomyce ... | 2006 | 16440212 |
| [chemical composition and bioactive compounds of flour of orange (citrus sinensis), tangerine (citrus reticulata) and grapefruit (citrus paradisi) peels cultivated in venezuela]. | the purpose of this research was to evaluate the chemical composition and some bioactive compounds in the peel's flour of some of the most consumed citrus fruits cultivated in venezuela. chemical composition as well as some trace elements, ascorbic acid, carotenoids dietary fiber, total polyphenols and their antiradical efficiency, using the 2,2-diphenyl-1-picrylhidracyl (dpph) were assessed in the dried peels of orange (citrus sinensis), tangerine (citrus reticulata) and white grapefruit (citru ... | 2005 | 16454058 |
| mining and characterizing microsatellites from citrus ests. | freely available computer programs were arranged in a pipeline to extract microsatellites from public citrus est sequences, retrieved from the ncbi. in total, 3,278 bi- to hexa-type ssr-containing sequences were identified from 56,199 citrus ests. on an average, one ssr was found per 5.2 kb of est sequence, with the tri-nucleotide motifs as the most abundant. primer sequences flanking ssr motifs were successfully identified from 2,295 citrus ests. among those, a subset (100 pairs) were synthesiz ... | 2006 | 16474971 |
| fruit coloration difference between fengwan, a late-maturing mutant and its original cultivar fengjie72-1 of navel orange (citrus sinensis osbeck). | fruit color is one of the most important external quality traits. mutants with different color are useful for the study of regulating mechanism of coloration progress. a novel mutant, fengwan, derived from fengjie72-1 navel orange (citrus sinensis l. osbeck), has the distinctive characteristic of the coloration delay of 30 d or so, with the change in total soluble solid (tss) and the ratio of tss/acidity. in order to understand the mechanism underlying the difference of coloration between the mu ... | 2006 | 16477128 |
| stomatal oscillations in orange trees under natural climatic conditions. | stomatal oscillations have been reported in many plant species, but they are usually induced by sudden step changes in the environment when plants are grown under constant conditions. this study shows that in navel orange trees (citrus sinensis) pronounced stomatal oscillations occur and persist under natural climatic conditions. | 2006 | 16478765 |
| calibration of sap flow estimated by the compensation heat pulse method in olive, plum and orange trees: relationships with xylem anatomy. | the compensation heat pulse method is widely used to estimate sap flow in conducting organs of woody plants. being an invasive technique, calibration is crucial to derive correction factors for accurately estimating the sap flow value from the measured heat pulse velocity. we compared the results of excision and perfusion calibration experiments made with mature olive (olea europaea l. 'manzanilla de sevilla'), plum (prunus domestica l. 'songal') and orange (citrus sinensis (l.) osbeck. 'cadener ... | 2006 | 16510387 |
| germin-like protein cit s 1 and profilin cit s 2 are major allergens in orange (citrus sinensis) fruits. | oranges are clinically relevant allergenic foods. to date, orange allergens have not been characterized in detail. the study is aimed at analyzing the sensitization profile in orange-sensitized subjects with and without clinical allergy, and to identify orange allergens. fifty-six sensitized subjects with self-reported reactions to orange were grouped into reactors (anaphylaxis or multiple episodes of immediate reactions and/or positive challenge tests) and non-reactors (negative open food chall ... | 2006 | 16521162 |
| gene characterization, analysis of expression and in vitro synthesis of dihydroflavonol 4-reductase from [citrus sinensis (l.) osbeck]. | dihydroflavonol 4-reductase (dfr, ec 1.1.1.219) catalyzes the reduction of dihydroflavonols to leucoanthocyanins, a key "late" step in the biosynthesis of anthocyanins. in this study we showed that a strong reduction in dfr expression occurs in the non-red orange cultivar (navel and ovale) compared to that of the red orange (tarocco) suggesting that the enzyme could be involved in the lack of production of anthocyanins. therefore, we isolated and compared the cdnas, the genomic clones, as well a ... | 2006 | 16524606 |
| fatty acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae. | arbuscule-forming fungi in the order glomales form obligate endomycorrhizal associations with plants that make them difficult to quantify, and taxonomy of the group is only beginning to be objectively understood. fatty acid methyl ester (fame) profiles were analyzed to assess the diversity and quantity of fatty acids in 53 isolates of 24 glomalean species. spores and endomycorrhizal roots of sudan grass (sorghum sudanense) and the citrus rootstock carrizo citrange (poncirus trifoliata x citrus s ... | 1995 | 16534923 |
| influence of immigration on epiphytic bacterial populations on navel orange leaves. | factors that influenced the increase in epiphytic bacterial population size on navel orange leaves during winter months were investigated to test the assumption that such populations were the result of multiplication on orange leaves. the population sizes of bacteria of different kinds, including ice nucleation-active (ice(sup+)) bacteria, were from 6- to 30-fold larger on leaves of navel orange trees adjacent to other plant species than on trees growing near other citrus species. total and ice( ... | 1996 | 16535384 |
| efficacy of bacillus thuringiensis against phyllocnistis citrella (lepidoptera: phyllocnistidae). | the purpose of this work was to analyze the efficacy of bacillus thuringiensis berliner on the control of phyllocnistis citrella stainton (lepidoptera: phyllocnistidae) in laboratory and field trials. in the laboratory, four b. thuringiensis were used: dipel (commercial formulation) tested at the concentrations of 25 x 10(6) and 25 x 10(8) spores/ml and the isolates k, 6, and 15 (collections of the university of azores) at the concentration of 25 x 10(6) spores/ ml. a surfactant solution of nono ... | 2005 | 16539108 |
| developing the sterile insect technique for cryptophlebia leucotreta (lepidoptera: tortricidae): influence of radiation dose and release ratio on fruit damage and population growth in field cages. | the effect of radiation dose and different release ratios of treated (t) to untreated (u) cryptophlebia leucotreta (meyrick) (lepidoptera: tortricidae), on the incidence of fruit damage, the competitiveness of the treated males, and population growth was examined inside field cages. navel orange trees were individually enclosed in large nylon mesh cages. newly emerged adult moths treated with either 150 or 200 gy of gamma radiation were released into the cages at ratios of 5t:1u or 10t:1u. the f ... | 2005 | 16539115 |
| analysis of microsatellites in citrus unigenes. | simple sequence repeats (ssrs) were investigated in the unigene sequences from expressed sequence tags (est) of sweet orange (citrus sinensis osbeck), trifoliate orange (poncirus trifoliata raf.) and other citrus species and cultivars. a total of 37 802 citrus unigene sequences corresponding to 23.29 mb were searched, resulting in the identification of 8,218 ssrs. among them there were 4,913 (59.8%) mono-, 1,419 (17.3%) di-, 1,709 (20.8%) tri-, 114 (1.39%) tetra-, 23 (0.28%) penta- and 40 (0.49% ... | 2006 | 16625833 |
| absorption of hydrocyanic acid by valencia-orange fruits and leaves fumigated at atmospheric pressure and at partial vacuum. | 1945 | 16653971 | |
| ether-soluble organic acids of mature valencia orange leaves. | 1947 | 16654097 | |
| the influence of rootstock on the mineral composition of valencia orange leaves. | 1949 | 16654236 | |
| the response of young valencia orange trees to differential boron supply in sand culture. | 1951 | 16654340 | |
| effect of differential nitrogen, potassium, and magnesium supply to young valencia orange trees in sand culture on mineral composition especially of leaves and fibrous roots. | 1954 | 16654674 | |
| cyanide & chelating agent effects on in vitro co(2) fixation in sweet orange leaves. | 1961 | 16655460 | |
| effects of calcium, potassium, & magnesium on oxalic, malic, & citric acid content of valencia orange leaf tissue. | 1961 | 16655480 | |
| regreening of valencia orange as influenced by potassium gibberellate. | 1962 | 16655704 | |
| respiratory response, ethylene production, and response to ethylene of citrus fruit during ontogeny. | the initial respiratory rates at 20 centrigrade of detached oranges (valencia and navel), grapefruit, and lemons decreased during ontogeny. small attached oranges respired at the same rate as detached fruits of the same weight, and cutting the pedicel produced no shock or injury stimulus to the respiratory rate. small oranges and grapefruit (average weight about 15 grams) showed pseudoclimacteric respiratory patterns and produced ethylene. the height of the respiratory rise and the amount of eth ... | 1970 | 16657319 |
| water potential components in growing citrus fruits. | growing navel orange fruits (citrus sinensis) 5.4 to 5.7 centimeters in diameter were used as a model system to determine the effects of transpiration and carbohydrate translocation on water and osmotic potentials in fruit tissues. evidence supported the hypothesis that osmotic potential in the vesicles would be affected little by changes in transpiration or carbohydrate translocation because the vesicles are anatomically isolated from the transpiration stream and are at the end of the carbohydr ... | 1970 | 16657407 |
| extensibility of pericarp tissue in growing citrus fruits. | the tensile force existing in the pericarp of a growing citrus (citrus sinensis) fruit 17 to 19 centimeters in circumference was sufficiently high to cause a 3% shrinkage of the pericarp when it was excised. when a fruit was cut along the equator to the central axis, shrinkage of the pericarp resulted in the formation of a wedge-shaped gap at the cut. stretch modulus of the pericarp was determined by measuring the force required to stretch excised strips of tissue to 1% longer than their excised ... | 1970 | 16657546 |
| changes in cellulase and pectinase activities in fruit tissues and separation zones of citrus treated with cycloheximide. | cellulase activity increased in separation-zone tissues 1 day after "valencia" orange (citrus sinensis [l.] osbeck) was treated with 20 micrograms per milliliter cycloheximide. exocellulase was detected only in the separation zones of treated fruit, whereas endocellulase was present in zones from both treated and control fruit. endocellulase activity in separation-zone tissue of treated fruit was nearly three times as great as that in control tissues. cellulase activity was restricted to separat ... | 1973 | 16658383 |
| diurnal variations in photosynthetic products and nitrogen metabolism in expanding leaves. | expanding leaves of capsicum frutescens l. cv. california wonder, cucumis melo l. cv. hales best, and citrus sinensis l. osbeck cv. washington navel showed a marked diurnal periodicity in the incorporation of (14)c from photosynthetically fixed (14)co(2) into amino acids. incorporation was virtually nil at the beginning of the photoperiod, reached a maximum in the 6th to 7th hour and decreased during the latter part of the photoperiod. in capsicum frutescens this was apparently a reflection of t ... | 1973 | 16658402 |
| effect of water stress on ethylene production by detached leaves of valencia orange (citrus sinensis osbeck). | detached leaves of valencia oranges, citrus sinensis osbeck, emanated ethylene at markedly higher rates and contained more endogenous ethylene when placed under water stress at 55% relative humidity than when placed in water-saturated air. water stress induced defoliation from detached branches. relieving the water stress of such leaves by transfer to a mist chamber resulted in lowering of the rates of ethylene emanation to the level occurring in leaves maintained continuously in a mist chamber. ... | 1974 | 16658805 |
| efficiency and regulation of water transport in some woody and herbaceous species. | the efficiency with which plants transport water is related to the water potential differences required to drive water fluxes from the soil to the leaf. a comparative study of two woody and three herbaceous species (citrus sinensis l. cv. koethen, pyrus kawakami l., helianthus annuus l. cv. mammoth russian, capsicum frutescens l. cv. yolo wonder, and sesamum indicum l. cv. glauca) indicated contrasts in water transport efficiency. depression of leaf water potential in response to transpiration i ... | 1974 | 16658853 |
| metabolism of uronic acids in plant tissues: partial purification and properties of uronic acid oxidase from citrus leaves. | a new enzyme, named uronic acid oxidase, was extracted and purified 67-fold by (nh(4))(2)so(4) fractionation and cm-sephadex column chromatography from ethylene-treated shamouti orange (citrus sinensis l. osbeck) leaves. the enzyme catalyzes the oxidation of d-galacturonic acid and d-glucuronic acid to the corresponding hexaric acids in the presence of molecular oxygen with the production of h(2)o(2). the ph optimum for the oxidation of d-galacturonic acid and d-glucuronic acid is between 7 and ... | 1975 | 16659133 |
| the effects of ph, ionic strength, and ethylene on the extraction of cellulase from abscission zones of citrus leaf explants. | the solubility of cellulase extracted from the abscission zones of citrus leaf explants (citrus sinensis l. osbeck) in sodium phosphate buffer depends on the ph of the extracting solution and, to a lesser extent, on the ionic strength. by increasing molarity from 0.01 to 0.16, the solubility of cellulase increased from 51% to 89% at ph 6.1 and from 70% to 98% at ph 7. in all cases, residual cellulase was further extracted from the pellet by buffer containing 1 m nacl. most of the enzymic activit ... | 1975 | 16659196 |
| partial purification of a growth factor from orange juice which affects citrus tissue culture and its replacement by citric acid. | orange (citrus sinensis l. osbeck) tissue cultures required a supply of orange juice to the medium for their vigorous growth. the growth-promoting activity of juice seemed to involve both cell division and cell enlargement. juice had no promotive activity in bioassays for auxins, gibberellins, and cytokinins. the growth promoting activity of juice was mostly transferred into 1-butanol upon partition at ph 2. gas chromatographic analysis of this acid 1-butanol fraction revealed large amounts of c ... | 1975 | 16659287 |
| cellulase activity, endogenous abscisic acid, and ethylene in four citrus cultivars during maturation. | at maturity, the fruit of two early maturing orange cultivars, hamlin and pineapple (citrus sinensis [l.] osbeck), contained more ethylene and abscisic acid than the late maturing valencia and lamb summer (c. sinensis [l.] osbeck) cultivars. ethylene (up to 95 nl/l in internal atmosphere) and abscisic (50 mug/kg dry weight flavedo) increased most rapidly in pineapple, leading to increased cellulase activity and loosening of the fruit. fruit of the two late maturing cultivars contained less than ... | 1975 | 16659390 |
| involvement of endogenous ethylene in the induction of color change in shamouti oranges. | reducing the level of endogenous ethylene in detached shamouti oranges (citrus sinensis l. osbeck) by means of subatmospheric pressure did not alter the rate of chlorophyll destruction and color changes during the first 8 days after harvest in the presence or absence of exogenous ethylene. reducing the activity of ethylene by means of co(2)-known to be a competitive inhibitor for ethylene-inhibited chlorophyll destruction and color change in shamouti oranges ventilated with ethylene, but had no ... | 1976 | 16659580 |
| ethylene-induced formation of aba in citrus peel as related to chloroplast transformations. | when mature green harvested shamouti oranges (citrus sinensis l. osbeck) were exposed to 35 mul/liter of ethylene, a 3-fold increase in free abscisic acid (aba) of the flavedo could be detected after 12 hours and a 10-fold increase after 24 hours, while chlorophyll destruction did not exceed 20%. the increase in free aba continued up to 24 hours and leveled off. bound aba accumulated strongly after 24 hours suggesting that excess of free aba was being converted into the bound form. similar incre ... | 1976 | 16659682 |
| endogenous abscisic acid in relation to bud growth in alternate bearing ;valencia' orange. | an investigation was conducted into the relation of aba (cis-trans-abscisic acid) in the dormant buds of alternate bearing ;valencia' orange (citrus sinensis [l.] osbeck) trees. aba did not appear to be related to alternate bearing but t-aba (2-transabscisic acid) did. there was 5- to 10-fold more t-aba than aba in the buds. there was more t-aba in the buds of the "on" trees than in the buds of the "off" trees, and a drastic drop in t-aba in both types of buds as spring growth approached. bud do ... | 1976 | 16659743 |
| differential counteraction of ethylene effects by gibberellin a(3) and n(6)-benzyladenine in senescing citrus peel. | treatment of mature citrus fruit (citrus sinensis) with ethylene induced rapid chlorophyll destruction, a rise in respiration, a release of free amino acids, an accumulation of reducing sugars, and an appearance of phenylalanine ammonia lyase activity. gibberellin a(3) (ga(3)) and n(6)-benzyladenine (ba) opposed the effects of ethylene on chlorophyll, amino acids, and to a lesser extent, reducing sugar levels. the ethylene-induced respiratory rise was only slightly modified by ga(3) and ba. phen ... | 1977 | 16659815 |
| differential effects of sucrose, abscisic acid, and benzyladenine on shoot growth and callus formation in the abscission zone of excised citrus buds. | the omission of sucrose from the basal medium stimulated callus formation in bud explants of citrus sinensis (l.) osbeck. moreover, it increased the abscisic acid-induced callus proliferation reported earlier in the presence of 5% sucrose (altman and goren, physiol. plant. 32: 55, 1974). the inhibition of callus formation by the addition of sucrose was not due to the high osmotic potential of the medium. benzyladenine induced callus formation slightly, in all sucrose concentrations up to 5%. the ... | 1977 | 16660013 |
| abscission of citrus leaf explants: no correlation with naphthaleneacetic acid conjugation in the abscission zone. | the role of alpha-naphthaleneacetic acid (naa) in the control of abscission in citrus (citrus sinensis l. osbeck) leaf explants and its conjugation were studied in non-aged and 24-hour-aged explants. dipping non-aged explants in 1.5 micromolar naa for 15 minutes immediately after excision did not delay abscission whereas 150 micromolar naa effectively delayed it. as incubation time was prolonged up to 24 hours after excision, the delaying effect of both concentrations gradually increased. in gen ... | 1978 | 16660505 |
| citrus tissue culture: stimulation of fruit explant cultures with orange juice. | in vitro growth of explant (juice vesicle or albedo tissues) cultures from citron (citrus medica), lemon (c. limon), grapefruit (c. paradisi), sweet orange (c. sinensis), and mandarin (c. reticulata) fruits was stimulated by addition of orange juice (10% v/v optimum) to a basal medium containing murashige and skoog salts, 50 grams per liter sucrose, 100 milligrams per liter myo-inositol, 5 milligrams per liter thiamine.hcl, 2 milligrams per liter 2,4-dichlorophenoxyacetic acid and 0.5 milligrams ... | 1978 | 16660631 |
| role of ethylene in abscisic acid-induced callus formation in citrus bud cultures. | the induction of callus formation in cultured buds of shamouti orange (citrus sinensis [l.] osbeck) by abscisic acid (aba) is a multiphasic process. (altman, and goren 1974 physiol plant 32: 55.) a study of the mediation by ethylene on this effect of aba was undertaken. it was found that: (a) ethylene and (2-chloroethyl) phosphonic acid, as well as aba, induced callus formation; (b) callus induction is best attained when explants are exposed to ethylene during the 1st day after excision; and (c) ... | 1979 | 16660713 |
| inhibition of polar indole-3-acetic acid transport by cycloheximide. | cycloheximide inhibits polar indoleacetic acid transport in midrib tissues of leaves of citrus (citrus sinensis [l.] osbeck) and poplar (populus deltoides bartr.) as measured by the donor-receiver agar cylinder technique. it appears that the mechanism of auxin transport inhibition by cycloheximide consists in arresting protein synthesis and not in the disruption of energy flow. the interpretation of the data takes into account the involvement of either a carrier protein or auxin-induced proton e ... | 1979 | 16660887 |
| accumulation of free proline in citrus leaves during cold hardening of young trees in controlled temperature regimes. | free proline increased in leaves of orange (citrus sinensis [l.] osb. cv. valencia) and grapefruit (citrus paradisi macfad. cv. star ruby) trees on a wide range of citrus rootstocks during cold hardening. increases in sugars accompanied proline accumulation. during cold hardening, the rate of proline accumulation was greater in old than in young leaves. in leaves of grapefruit trees kept in the dark during cold hardening, neither proline nor sugars increased and the degree of cold hardiness was ... | 1979 | 16660980 |
| citrus tissue culture : auxins in relation to abscission in excised pistils. | an in vitro bioassay for chemicals that affect citrus abscission was used to identify three inhibitors of stylar abscission in lemon pistil explants incubated on defined nutrient media. the three inhibitors (picloram, 4-chlorophenoxyacetic acid, and 3,5,6-trichloropyridine-2-oxyacetic acid) are all auxins, and the most potent of them (i.e. picloram) was found to be at least 10 times more active in the bioassay than 2,4-dichlorophenoxyacetic acid. picloram (2 micromolar) also was shown to be effe ... | 1981 | 16661819 |