Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| experimentally produced calf pneumonia. | experimental pneumonia was produced in calves by the endobronchial inoculation of pneumonic lung homogenates. irradiated homogenates produced minimal pneumonia. ampicillin treatment of the homogenates and the experimental calves reduced the extent of pneumonia. treatment with tylosin tartrate prevented experimental pneumonia. these results suggest that the total pneumonia was due to organisms susceptible to tylosin tartrate and that the residual pneumonia remaining after ampicillin treatment was ... | 1976 | 1265354 |
| effect of cerulenin on growth and lipid metabolism of mycoplasmas. | cerulenin markedly inhibited the growth of acholeplasma laidlawii. a. axanthum and a. granularum were less susceptible, whereas the sterol-requiring mycoplasma species examined showed very little susceptibility. the inhibition was not reversed by the addition of long-chain fatty acids to the medium. at a concentration of 20 mug/ml, cerulenin inhibited the incorporation of [(14)c]acetate into a. laidlawii membrane lipids, but it had no effect on either protein or nucleic acid biosynthesis. cerule ... | 1976 | 1267428 |
| replication of mycoplasmavirus mvl51. iv. inhibition of viral synthesis by rifampin. | the effect of rifampin on the replication of mvl51, a bullet-shaped mycoplasmavirus with single-stranded circular dna of molecular weight 2 x 10(6), has been examined in a rifampin-resistant host cell. rifampin does not block the early steps in mvl51 infection but does decrease the total amount of parental viral dna taken up. the single-stranded parental viral dna that enters the cell is found in membrane-associated, double-stranded dna replicative forms i and ii. rifampin had no significant eff ... | 1976 | 1271535 |
| infectious bovine keratoconjunctivitis: experimental induction of infection in calves with mycoplasmas and moraxella bovis. | eyes of 14 calves were exposed by conjunctival instillation to cultures of either mycoplasma conjunctivae (6 calves) or acholeplasma laidlawii (8 calves). calves were observed for clinical signs of infectious bovine keratoconjunctivitis (ibk), and eyes were examined for the test organisms by bacteriologic cultural technique for 60 days. acholeplasma laidlawii became established in the eyes of 5 of 8 calves; m conjunctivae became established in the eyes of 4 of 6 calves. on day 28, eyes of 9 of t ... | 1976 | 1275332 |
| [localization and possible development cycle of mycoplasmas and bacterial l-forms in cell cultures]. | using vital stain chlorazol-black e the authors studied the localization and behaviour of 5 mycoplasma species and the stable l-form of beta-hemolytic streptococcus of group a in various continuous cell lines. mycoplasmas and l-forms had a definite evolution cycle in the cells. at first they are arranged extracellularly and on the surface of the cells, and then intracellularly where they multiply intensively; later they are again localized on the surface of the cells and extracellularly. this cy ... | 1976 | 1276447 |
| acholeplasma laidlawii has trna genes in the 16s-23s spacer of the rrna operon. | we amplified the 16s-23s rrna intergenic spacer region of acholeplasma laidlawii pg8 by polymerase chain reaction (pcr) and obtained two specific pcr products in different sizes. we have sequenced both pcr products and found that one of them has sequence homologous to the spacer trna genes in bacillus subtilis. this is the first evidence of trna genes between the 16s-23s rrna intergenic spacer regions in members of the class mollicutes. | 1992 | 1281149 |
| [the isolation of ribosomes from acholeplasma and the study of their physicochemical characteristics]. | ribosomes are produced from acholeplasma laidlawii pg-8 and a. laidlawii var. granulum str. 118. it is proved as possible to use the standard method of differential centrifugation for isolation of mollicute ribosomes. the physico-chemical properties of acholesplasma ribosomes are studied. the protein content for a. laidlawii pg-8 amounted to 39.6%, rrna content--60.4% and for a. laidlawii var. granulum--39.1 and 60.8%, respectively. the rna: protein ratio equals 1.5:1, the ratio of optic density ... | 1992 | 1284088 |
| [identification of mycoplasma by the growth inhibition of samples isolated from cell cultures]. | cell cultures must be continuously screened for the presence of mycoplasma because, although these microorganisms sometimes pass unnoticed, they may cause chromosomic alterations and interfere with viral replication, antibody and interferon production etc. the international organization for mycoplasmology (iom) recommends the isolation and identification of mycoplasma with a view to the detection of the origin of the infection and the improvement of the quality of the cultures. in this paper, 37 ... | 1992 | 1307416 |
| influence of lipid composition on the orientational order in acholeplasma laidlawii strain b membranes: a deuterium nmr study. | 2h nmr techniques have recently been developed to determine the complete orientational order profile of lipid bilayers employing lipids containing perdeuteriated palmitic acid [lafleur, m., fine, b., sternin, e., cullis, p.r., & bloom, m. (1989) biophys. j. 56, 1037-1041]. in this work, these techniques have been applied to study order profiles in intact membranes derived from acholeplasma laidlawii strain b. it is shown that complete orientational order profiles can be readily obtained from the ... | 1992 | 1390762 |
| [the effect of physicochemical factors on the activity of dna-dependent dna polymerases in acholeplasma laidlawii pg-8]. | the biological and physico-chemical properties of dna-dependent dna-polymerases of acholeplasma laidlawii pg-8 have been studied. the optimal parameters of maximal enzymatic activity are determined. it is stated that n-ethylmaleimide in concentration of 1 mm activated dna-polymerase i by 52%, whereas dna-polymerase ii with reagent concentration of 0.5 mm demonstrated the peak of activity exceeding the control only by 10%. spermidine in concentration of 1.5 mm for the first form of dna-polymerase ... | 1992 | 1406382 |
| breeder turkey hens seropositive and culture-negative for mycoplasma synoviae. | four flocks of clinically normal turkey breeder hens were shown to have suspect and positive mycoplasma synoviae (ms) hemagglutination-inhibition (hi), enzyme-linked immunosorbent assay, and, in some cases, serum plate agglutination serology in the absence of ms isolation. in all cases, hi serology for mycoplasma gallisepticum (mg) and m. meleagridis was negative. acholeplasma laidlawii was isolated from some hens in each of these ms-seropositive culture-negative flocks. immunoblotting was used ... | 1992 | 1417615 |
| [tubular structures in mycoplasma gallisepticum and the localization of a tubulin-like protein]. | in all the strains of m. gallisepticum investigated, a protein with apparent molecular weight 40 kda was revealed by immunoblotting with polyclonal anti-calf brain tubulin antibodies and monoclonal anti-chicken alpha-tubulin antibodies. in other 8 investigated mycoplasma species no positive reactions with the same antibodies were found. the m. gallisepticum cells were examined under electron microscope on fine serial sections and on some sections going at different angles to the long cell axis. ... | 1992 | 1440927 |
| [the effect of mycoplasma contamination on the karyotypic structure of a skin fibroblast cell line from the indian muntjac]. | the karyotypic variability has been studied in a low chromosomal cell line of the indian muntjak skin fibroblasts contaminated with mycoplasma arginini r-16 and acholeplasma laidlawii a. the mycoplasmal contamination exerted influence on the cell distribution for the chromosome number. the frequency of the modal class cells with 7 chromosomes, having the main structure variant of the karyotype (msvk) 2 + 2 + 1 + 1 + 1, was seen to decrease, while the frequency of the submodal class cells with 6 ... | 1992 | 1440934 |
| construction of reca mutants of acholeplasma laidlawii by insertional inactivation with a homologous dna fragment. | mycoplasmas (class mollicutes) are wall-less prokaryotes phylogenetically related to gram-positive bacteria. this study describes the construction of reca mutants of the mycoplasma acholeplasma laidlawii. an internal fragment of the reca gene from a. laidlawii was cloned into a plasmid that does not replicate in this organism. when this plasmid construct was used to transform a. laidlawii, it inserted into the chromosome, disrupting the reca gene. the phenotype of the resulting reca mutant was c ... | 1992 | 1461941 |
| the nasal mycoplasmal flora of healthy calves and cows. | the nasal mycoplasmal flora of 270 healthy cows from 27 herds in the netherlands and 35 healthy calves from 7 of these herds was examined. various methods for isolating mycoplasmas were compared. the prevalence of the various species was as follows: ureaplasma diversum in 3 (9%) calves; mycoplasma dispar in 14 (40%) calves; m. bovis in 1 (3%) calf; m. bovirhinis in 23 (66%) calves and 16 (6%) cows; m. bovoculi in 8 (23%) calves and 53 (20%) cows; m. canis in 1 (3%) calf; m. equirhinis in 2 (1%) ... | 1992 | 1462726 |
| effect of glycolipids on the phase behavior and dynamic properties of phospholipid liposomes. | the glycolipids of acholeplasma laidlawii aih089 membranes were identified and purified. the effect of monoglucosyldiacylglycerol (mgdg) and diglucosyldiacylglycerol (dgdg) on the thermotropic behavior of multilamellar vesicles of dipalmitoylphosphatidylcholine (dppc), dipalmitoylphosphatidylglycerol (dppg) has been investigated by high sensitivity differential scanning calorimetry. the main transition peaks were broadened, the enthalpies were decreased. dgdg caused the decrease in the transitio ... | 1992 | 1510663 |
| the enzymatic synthesis of membrane glucolipids in acholeplasma laidlawii. | in membranes of the prokaryote acholeplasma laidlawii, the physiological regulation of the two major membrane lipids, monoglucosyldiacylglycerol (mglcdag) and diglucosyldiacylglycerol (dglcdag), is governed by factors affecting the equilibria between lamellar and non-lamellar phases of the membrane lipids. the synthesis of the glucolipids is considered to be a two-step glucosylation: (i) dag+udp-glc----mglcdag+udp; and (ii) mglcdag+udp-glc----dglcdag+upd. this was corroborated by in vivo pulse l ... | 1992 | 1533160 |
| the effect of cholesterol and epicholesterol on the activity and temperature dependence of the purified, phospholipid-reconstituted (na+ + mg2+)-atpase from acholeplasma laidlawii b membranes. | the (na+ + mg2+)-atpase purified from acholeplasma laidlawii b membranes was reconstituted into large, unilamellar vesicles formed from dimyristoylphosphatidylcholine (dmpc) and varying amounts of cholesterol or epicholesterol. the atp hydrolytic activity of the reconstituted enzyme was then determined over a range of temperatures and the phase state of the dmpc in the atpase-containing vesicles was characterized by high-sensitivity differential scanning calorimetry. in the vesicles containing o ... | 1992 | 1535512 |
| evolutionary relationships of a plant-pathogenic mycoplasmalike organism and acholeplasma laidlawii deduced from two ribosomal protein gene sequences. | the families within the class mollicutes are distinguished by their morphologies, nutritional requirements, and abilities to metabolize certain compounds. biosystematic classification of the plant-pathogenic mycoplasmalike organisms (mlos) has been difficult because these organisms have not been cultured in vitro, and hence their nutritional requirements have not been determined nor have physiological characterizations been possible. to investigate the evolutionary relationship of the mlos to ot ... | 1992 | 1556079 |
| [the chromatographic properties of the dna-dependent dna polymerases from acholeplasma laidlawii pg-8]. | the dna-dependent dna-polymerase (dna polymerase i which is not sorbed on the column with deae-cellulose, and dna-polymerase ii, which is absorbed by this column and is eluted from it by 0.3 m of nacl), have been isolated from acholeplasma laidlawii pg-8. dna-polymerase i in homogeneous state was obtained as a result of the stepwise treatment by heparin-sepharose (elution at 0.35 m of nacl) and poly-u-sepharose (elution at 0.3 m of nacl). it was presented on the electrophoregram by one polypepti ... | 1992 | 1584084 |
| [rnase substrate specificity in acholeplasma laidlawii pg-8]. | a substrate specificity of rnases of a. laidlawii pg-8 to polynucleotides - poly (c), poly (u), poly (a) has been studied. due to the data obtained both intracellular and extracellular rnases of a. laidlawii possess similar specificity to different polynucleotides. both rnases preferentially break cytidine-bonds. specificity of the studied enzymes in respect to polyuridylic and polyadenylic acids was less expressed. | 1992 | 1584085 |
| some observations on mycoplasma-cidal activity of horse serum. | 1992 | 1606266 | |
| behaviour of a glycosphingolipid with unsaturated fatty acid in phosphatidylcholine bilayers. | n-(oleoyl)galactosylceramide with perdeuterated acyl chain was prepared by partial synthesis, and studied by wide line 2h-nmr in phospholipid liposomes. spectra were obtained for low glycolipid concentrations in bilayers of dimyristoyl-, distearoyl-, and 1-palmitoyl-2-oleoylphosphatidylcholines. in an attempt to isolate the effects of glycosphingolipid fatty acid cis unsaturation on glycolipid behaviour in membranes, spectral findings related to the above species were compared to literature nmr ... | 1992 | 1616922 |
| isoprenoid modification of proteins distinct from membrane acyl proteins in the prokaryote acholeplasma laidlawii. | isoprenylation is an important posttranslational modification that affects the activity, subunit interactions and membrane anchoring of different eukaryotic proteins. the small, cell-wall-less prokaryote acholeplasma laidlawii has more than 20 membrane acyl-proteins enriched in myristoyl and palmitoyl chains. radioactive mevalonate, a precursor to isoprenoids, was incorporated into several specific membrane proteins of 20 to 45 kda and two soluble proteins of 23-25 kda, respectively. no acyl pro ... | 1992 | 1616923 |
| the electrophoretic mobility of gram-negative and gram-positive bacteria: an electrokinetic analysis. | the electrophoretic mobility (epm) of a variety of gram-negative and gram-positive bacteria was measured with a penkem s3000 analyser. under standard growth conditions and neutral ph all cells displayed a negative epm. the polysaccharide capsules of escherichia coli strains k1, k5, k29 and k30 generated the highest epm; to a lesser and varying degree o-antigens with charged groups and core lipopolysaccharides also contribute to the net epm. very little negative epm was measured in suspension cul ... | 1990 | 1696306 |
| fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody. | the detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. in our laboratory, monoclonal antibodies (mabs) have been prepared to acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, mycoplasma arginini and mycoplasma salivarium. 30 mabs were obtained and one of these, designated ccm-2, was shown to bind to all five mycoplasma species. it also b ... | 1990 | 1697315 |
| immunological properties of antibodies against mg(2+)-atpase from acholeplasma laidlawii membranes. | in the present study, antibodies were raised against the mg(2+)-atpase and the immunological relationships between the enzyme and other atpase from a variety of biological membranes were determined. the anti mg(2+)-atpase antiserum inhibited 85% of the enzyme activity from a. laidlawii membranes. we demonstrate a specific selectivity of mg(2+)-atpase antiserum for antigenic determinants of the a. laidlawii membranes. immunoblot studies of a. laidlawii membrane peptides indicated labeling of five ... | 1991 | 1714730 |
| evolution of trnas and trna genes in acholeplasma laidlawii. | the genes for 22 trna species from acholeplasma laidawii, belonging to the class mollicutes (mycoplasmas), have been cloned and sequenced. sixteen genes are organized in 3 clusters consisting of eleven, three and two trna genes, respectively, and the other 6 genes exist as a single gene. the arrangement of trna genes in the 11-gene, the 3-gene and the 2-gene clusters reveals extensive similarity to several parts of the 21-trna or 16-trna gene cluster in bacillus subtilis. the 11-gene cluster is ... | 1991 | 1722304 |
| membrane properties of a plant-pathogenic mycoplasmalike organism. | in terms of biosystematics, the plant-pathogenic mycoplasmalike organisms (mlos) have been tentatively placed into the class mollicutes. certain physiological tests have been used to distinguish families within this class: the sterol-nonrequiring acholeplasmataceae differ from the sterol-requiring mycoplasmataceae in that the former are more resistant to lysis by digitonin and more sensitive to lysis in hypotonic salt solutions. to test mlos for these membrane properties and thus assist in their ... | 1992 | 1732203 |
| cloning and dna sequence of a mycoplasmal reca gene. | mycoplasmas are wall-less prokaryotes phylogenetically related to gram-positive bacteria. in order to investigate dna recombination in these organisms, we have cloned the reca gene from the mycoplasma acholeplasma laidlawii. dna sequence data indicate extensive homology between the a. laidlawii reca gene and reca genes from other bacteria, particularly bacillus subtilis. the reca sequences from three a. laidlawii strains (strains ja1, k2, and 8195) were compared, and surprisingly, the gene from ... | 1992 | 1732213 |
| identification and analysis of the genes coding for the putative pyruvate dehydrogenase enzyme complex in acholeplasma laidlawii. | a monospecific antibody recognizing two membrane proteins in acholeplasma laidlawii identified a plasmid clone from a genomic library. the nucleotide sequence of the 4.6-kbp insert contained four sequential genes coding for proteins of 39 kda (e1 alpha, n terminus not cloned), 36 kda (e1 beta), 57 kda (e2), and 36 kda (e3; c terminus not cloned). the n termini of the cloned e2, e1 beta, and native a. laidlawii e2 proteins were verified by amino acid sequencing. computer-aided searches showed tha ... | 1992 | 1735725 |
| membrane protein acylation. preference for exogenous myristic acid or endogenous saturated chains in acholeplasma laidlawii. | mycoplasmas are small bacteria without a cell wall, often found as surface parasites on eukaryotic cells. of the more than 200 membrane proteins from acholeplasma laidlawii resolved by two-dimensional page, 23 were covalently modified with acyl chains. these acyl proteins had lower pi values than average and were all labelled by different exogenously supplied radioactive fatty acids attached by o-ester bonds. the fatty acids were selectively incorporated in the order myristic acid (14:0) greater ... | 1992 | 1740134 |
| [the stability of the alkylating derivatives of oligodeoxyribonucleotides containing a cholesterol or phenazine radical added to the 3'-termination during their interaction with acholeplasma laidlawii pg-8]. | stability of alkylating derivatives of decathymidylates protected on the 3'-terminal by cholesterol and phenazine residues has been studied in the process of their interaction with cells of acholeplasma laidlawii pg-8. it is shown that the studied reagents are not split by nucleases of a. laidlawii pg-8 for the time necessary for alkylation of mycoplasma biopolymers. | 1991 | 1753886 |
| [the interaction of alkylating derivatives of oligodeoxyribonucleotides and their methylphosphonate analogs with mycoplasma cells]. | alkylating derivatives of decathymidylates and methylphosphonate analogs of oligodeoxyribonucleotides (mpao) were studied for their interaction with cells of acholeplasma laidlawii pg-8, mycoplasma capricolum california kid, m. pneumoniae fh and phytopathogenic strain (st. 118). it is shown that mpao of octa- and hexadecathymidylates as well as decathymidylates 3'-terminal modified by phenazine and cholesterol groupings are sorbed by mycoplasma cells and can penetrate inside the cells. efficienc ... | 1991 | 1753887 |
| identification of porcine mycoplasmas using commercial enzyme assay systems. | 1991 | 1760468 | |
| [cross reactive antigens of acholeplasma laidlawii and l-form of staphylococcus aureus]. | we demonstrated that the membrane of acholeplasma laidlawii pg8 and l-form of staphylococcus aureus, both of which induce cellular immunity in balb/c mice, were antigenically related each other. foodpad responses of the mice immunized with a mixture of either antigen and freund's complete adjuvant showed clearly a cross reaction when challenged with the other antigen. cross responses to incorporate 3h-thymidine to the spleen lymphocytes of the mice immunized with either antigen occurred in the p ... | 1991 | 1762175 |
| chloride fluxes across acholeplasma laidlawii membranes. | chloride fluxes across the cytoplasmic membrane of acholeplasma laidlawii were studied by using the chloride sensitive fluorescent dye, 6-methoxy-n-(sulfopropyl)quinolinium. chloride was found to penetrate the membrane passively. chloride flux was dependent upon the transmembrane electric potential. | 1991 | 1778416 |
| [the isolation and partial purification of 2 dna-dependent dna polymerases from acholeplasma laidlawii pg-8]. | two forms of dna-dependent dna-polymerase have been isolated and partially purified from the limited amount of biomass of cells acholeplasma laidlawii pg-8, a typical representative of genus acholeplasmataceae, as a result of successive chromatography on the columns with deae-cellulose de-52 and green a-sepharose. the first form of dna-polymerase is eluted from the ion-exchange column with nacl concentration of 0.1 m from the column with green a-sepharose of 0.27 m, while the second form-with na ... | 1991 | 1791778 |
| [the rnases of the mollicute acholeplasma laidlawii pg-8: its detection and accumulation dynamics]. | 1991 | 1791779 | |
| comparison of glycoproteins from ten human mycoplasma species. | multiple bands of glycoprotein, rare in procaryotes, were detected in ten human mycoplasma species by staining with periodic acid-schiff (pas) reagent after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). a major contaminant formed in hayflick medium (h medium), corresponding to an apparent molecular weight of about 80 kd, was eliminated by using the organisms grown in pplo broth supplemented with pplo serum fraction (p medium), except that m. genitalium and m. pneumoniae ... | 1991 | 1815100 |
| mystery swine disease in the netherlands: the isolation of lelystad virus. | in early 1991, the dutch pig-industry was struck by the so-called mystery swine disease. large-scale laboratory investigations were undertaken to search for the etiological agent. we focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. the mycoplasmas m. hyosynoviae, m. hyopneumoniae, and acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 were isolated from indi ... | 1991 | 1835211 |
| interaction of modified oligonucleotides with eukaryotic and mycoplasma cells and their fate inside the cells. | 1991 | 1841342 | |
| continuous distribution of mycoplasma genome sizes. | genome sizes of eleven strains of eight species of mollicutes mycoplasmataceae were investigated by pulsed-field gel electrophoresis. mycoplasma genomic sizes were determined from the sum of the sizes of fragments obtained after digestion of genomic dna with restriction endonucleases. the sizes of the fragments were determined by comparison of their electrophoretic mobilities with those of lambda dna concatemers. specific restriction endonucleases were chosen so that after digestion three to ten ... | 1991 | 1841633 |
| [the thermodynamic parameters of the conformational transitions in mycoplasma dna]. | dna preparations have been isolated from 10 strains of phytopathogenic mycoplasms and collection culture achole plasma laidlawii pg-8. thermodynamic parameters of denaturation changes in the secondary structure (transconformation) of dna of these mycoplasms have been determined. it is shown that denaturation temperature is 82.3-83.1 degree c and enthalpy of conformational dna transitions calculated per 1 g of dry substance is 56.2-61.9 j/g. changes in the enthalpy (delta h) and entropy (delta s) ... | 1991 | 1861653 |
| search for eukaryotic motility proteins in spirochetes: immunological detection of a tektin-like protein in spirochaeta halophila. | the serial endosymbiotic theory (set) for the spirochete origin of undulipodia (cilia and eukaryotic flagella) predicts that a greater number of axonemal proteins will show homology to spirochete than to other bacterial proteins. to continue testing, the set proteins associated with eukaryotic motility (tektin, centrin and calmodulin) were sought in spirochaeta halophila. western blot immunological detection techniques (for tektin and centrin) and two-dimensional gel analysis (for calmodulin) we ... | 1991 | 1863720 |
| [contamination of fish tissue cell cultures by mycoplasma]. | the inoculated and primary cell cultures of fish (carp, salmon, and sturgeon) have been studied. acholeplasma typed as a. laidlawii in terms of its biochemical properties shown in inhibited metabolism has been isolated from 19 samples. the authors consider the source of acholeplasma-induced contamination of the cell cultures under study. | 1991 | 1927041 |
| fusion of mycoplasmas: the formation of cell hybrids. | poly(ethylene glycol) (peg 8000) can induce cell-cell fusion of mycoplasma capricolum cells, and it can promote the formation of intergeneric hybrids of various mycoplasma, acholeplasma and spiroplasma species. the extent of fusion was quantitatively evaluated by following the dequenching of octadecylrhodamine fluorescent label incorporated into donor cell membranes after their incubation with recipient cells. the results of dequenching experiments were confirmed by electron microscopy, as well ... | 1991 | 1936938 |
| the genome size of a plant-pathogenic mycoplasmalike organism resembles those of animal mycoplasmas. | the genome size of a mycoplasmalike organism was determined by comparing fluorescence intensities of restriction fragments. its genome size was similar to that of mycoplasma gallisepticum and much smaller than that of acholeplasma laidlawii. although the genome size is "mycoplasmalike," other molecular data indicate a closer evolutionary relationship to a. laidlawii. | 1991 | 2002012 |
| evidence that mycoplasmas, gram-negative bacteria, and certain gram-positive bacteria share a similar protein antigen. | it was demonstrated that mycoplasmas, gram-negative bacteria, and certain gram-positive bacteria share a similar protein antigen with a molecular weight ranging from 42,000 to 48,000. western blotting (immunoblotting) with an antibody specific to a 43-kda membrane protein of mycoplasma fermentans showed the existence of this protein antigen in all mycoplasma spp. tested (14 species), acholeplasma laidlawii (1 strain), and gram-negative bacteria (8 species) but only in staphylococcus aureus of fo ... | 1991 | 2007558 |
| order and dynamics in mixtures of membrane glucolipids from acholeplasma laidlawii studied by 2h nmr. | the two dominant glucolipids in acholeplasma laidlawii, viz., 1,2-diacyl-3-o-(alpha-d-glucopyranosyl)-sn-glycerol (mglcdg) and 1,2-diacyl-3-o-[alpha-d-glucopyranosyl-(1----2)-o-alpha-d-glucopyranosyl ]- sn-glycerol (dglcdg), have markedly different phase behavior. mglcdg has an ability to form nonlamellar phases, whereas dglcdg only forms lamellar phases. for maintenance of a stable lipid bilayer, the polar headgroup composition in a. laidlawii is metabolically regulated in vivo, in response to ... | 1991 | 2036360 |
| [the interaction of the rna-polymerases of a phytopathogenic strain of mycoplasma with oligodeoxyribonucleotides associated with gene promoter regions]. | 1990 | 2090419 | |
| evaluation of different hybridization procedures for the detection of mycoplasma contamination in cell cultures. | cell culture samples were analysed for mycoplasma contaminations with two different dna probes which have been described earlier. one probe (the h900 probe), derived from the 23s rrna gene of mycoplasma hyorhinis, cross-hybridized with virtually all mycoplasmas (including the acholeplasmas). the other probe (the t2 probe), derived from a protein gene of acholeplasma laidlawii, cross-hybridized with most acholeplasmas. the two probes were compared in three different direct filter hybridization pr ... | 1990 | 2107395 |
| glycosidase activities of mycoplasmas. | the activities of alpha- and beta-glucosidase, beta-galactosidase and beta-n acetylglucosaminidase were assessed at acidic ph by fluorimetry using the appropriate 4-methylumbelliferyl substrate in four mycoplasma species (m. pneumoniae, m. gallisepticum, m. hominis and m. capricolum) and in acholeplasma laidlawii. the glycosidase activities were in a low range (0.1-4.2 nmole per h per mg protein) with the exception of higher activities of beta-n-acetylglucosaminidase in a. laidlawii. the enzyme ... | 1990 | 2119590 |
| studies on the purified na+,mg(2+)-atpase from acholeplasma laidlawii b membranes: a differential scanning calorimetric study of the protein-phospholipid interactions. | the purified na+,mg2(+)-atpase from the acholeplasma laidlawii b plasma membrane was reconstituted with dimyristoyl phosphatidylcholine and the lipid thermotropic phase behavior of the proteoliposomes formed was investigated by differential scanning calorimetry. the effect of this atpase on the host lipid phase transition is markedly dependent on the amount of protein incorporated. at low protein/lipid ratios, the presence of increasing quantities of atpase in the proteoliposomes increases the t ... | 1990 | 2140945 |
| genetic exchange of transposon and integrative plasmid markers in mycoplasma pulmonis. | matings of genetically marked derivatives of mycoplasma pulmonis resulted in the exchange of chromosomal dna and the appearance of doubly marked transconjugants. transposons tn916 and tn4001, and a series of integrative plasmids derived from their cloned antibiotic resistance genes, were used to construct antibiotic-resistant mycoplasmal derivatives to examine this phenomenon at the molecular level. genetic exchange occurred on agar surfaces at frequencies ranging from 3.3 x 10(-4) to 6.4 x 10(- ... | 1990 | 2158966 |
| terminal redundancy and circular permutation of mycoplasma virus l3 dna. | this communication reports the physical map of mycoplasma virus l3 (mv-l3) dna derived from restriction patterns obtained by digestion with seven different restriction endonucleases. the length of the restriction map is 36,200 bp in contrast to the contour length of native mv-l3 dna molecules which is 39,400 bp as determined by electron microscopy. the difference in length of 3,200 bp (corresponding to 8.1% of the native viral dna contour length) is explained by terminal redundancy. it was possi ... | 1990 | 2212995 |
| ageing of procaryotes. acholeplasma laidlawii as an object for cell ageing studies: a brief note. | it was established that a. laidlawii cell viability in the stationary phase of culture development decreases according to the gompertz law. it was shown that the "stationary" ageing of a. laidlawii cells is accompanied by the lower intensity of phosphorus-containing compounds biosynthesis, decreased rate of membrane proteins synthesis and degradation and also by accumulation of dna single-strand breaks. the biochemical changes found in ageing a. laidlawii cells are similar to ageing manifestatio ... | 1990 | 2214893 |
| pulse-field electrophoresis indicates full-length mycoplasma chromosomes range widely in size. | full-size linear chromosomes were prepared from mycoplasmas by using gamma-irradiation to introduce one (on average) double-strand break in their circular chromosomes. chromosome sizes were estimated by pulsed-field gel electrophoresis (pfge) from the mobilities of these full-length molecules relative to dna size references. sizes estimated for ureaplasma urealyticum t960 and 16 mycoplasma species ranged from 684 kbp (m. hominis) to 1315 kbp (m. iowae). using this sample, we found no correlation ... | 1990 | 2216718 |
| effects of age, environmental temperature and relative humidity on the colonization of the nose and trachea of calves by mycoplasma spp. | nasal and tracheal swabs sequentially collected from three groups of eight calves between the ages of 1 and 98 days indicated that the nose and trachea were colonized by mycoplasma spp. during the first weeks of life. over 92% of all calves harboured mycoplasma spp. in their noses when they were 2 weeks old, the rate of recovery falling gradually thereafter. the peak period of recovering mycoplasmas from the noses and tracheas of calves was at 6 weeks old. m. bovirhinis, m. arginini and acholepl ... | 1990 | 2224487 |
| cholesterol accumulation in plasma membrane and changes of membrane enzyme activity of acholeplasma laidlawii cells during culture ageing. | the data presented in this report show that the microviscosity of the plasma membrane and the cholesterol/phospholipid molar ratio of its lipid bilayer increases during ageing of acholeplasma laidawii cultures. at the same time the age changes of other lipid components content do not correlate with the change of membrane viscosity. it is also shown that membrane enzyme activities of mycoplasma cell decrease with age. these results confirm the membrane hypothesis of ageing according to which incr ... | 1990 | 2232909 |
| differences in accumulation of radiolabeled amino acids and polyamines by mycoplasma and acholeplasma species. | four species in the order mycoplasmatales, mycoplasma capricolum, mycoplasma hominis, mycoplasma arginini, and acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines. the use of a novel high-molecular-weight (hmw) medium, from which molecules of less than 12,000 molecular weight had been removed by extensive dialysis, allowed us to discern significant differences among the species in their relative accumulations of [3h]methionine and [3h]leu ... | 1990 | 2275861 |
| isolation and characterization of mycoplasma virus l3 temperature-sensitive mutants. | mycoplasma virus l3 virions are morphologically similar to coliphage t7, contain linear double-stranded dna of about 39 kilobase pairs, and produce a nonlytic cytocidal infection in acholeplasma laidlawii host cells. following nitrous acid mutagenesis, ninety-eight l3 temperature-sensitive (ts) mutants were isolated from a total of 57,000 plaque-forming units (pfu), using 37 degrees c as the permissive temperature and 41 degrees c as the nonpermissive temperature, with reversion frequencies of 1 ... | 1990 | 2277590 |
| plasmid transformation and replica filter plating of acholeplasma laidlawii. | the restriction deficient mutant 8195 of acholeplasma laidlawii strain ja1 was transformed by the promiscuous streptococcal plasmid vector pnz18 at a frequency of 4 x 10(-4)/cfu. the plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin. transformed primary colonies were easily recognized due to a different colony morphology. replica filter plating, previously not obtained with mycoplasmas, was achieved using pnz1 ... | 1990 | 2283030 |
| heat shock response in mycoplasmas, genome-limited organisms. | we have measured the effect of heat shock on three mycoplasmas (acholeplasma laidlawii k2 and ja1 and mycoplasma capricolum kid) and demonstrated the induction of mycoplasma heat shock proteins under these conditions. increased synthesis of at least 5 heat shock proteins in a. laidlawii k2, 11 heat shock proteins in a. laidlawii ja1, and 7 heat shock proteins in m. capricolum was observed by electrophoretic analysis of proteins from heat-shocked cells in sodium dodecyl sulfate-polyacrylamide gel ... | 1990 | 2318804 |
| human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis. | contour clamped homogeneous field (chef) agarose gel electrophoresis (age), ramped to give linear separation of dna molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic dna of the serotype standard strains of the human genital mollicutes, ureaplasma urealyticum relative to yeast chromosomal dna markers. indicated genome sizes (in kbp) were 760 for the four biotype 1 strains and 840-1140 for eleven biotype 2 strains. other estimates were: 720 for myc ... | 1990 | 2326188 |
| enhancement of cytotoxicity of active macrophages by mycoplasma: role of mycoplasma-associated induction of tumor necrosis factor-alpha (tnf-alpha) in macrophages. | tumor necrosis factor-alpha (tnf-alpha)-inducing activity of several mycoplasmas including mycoplasma pneumoniae, a causative agent in human respiratory infectious diseases, was investigated. purified peritoneal macrophages from balb/c mice markedly enhanced their cytotoxic activity to meth a cells, when cultured with either viable or non-viable mycoplasmas. the supernatants of the macrophage culture with mycoplasmas, m. pneumoniae and acholeplasma laidlawii, showed the potent cytotoxic activity ... | 1990 | 2352496 |
| cholesterol oxidation on fluorocarbon emulsion surface leads to the formation of 7-peroxycholesterol. | formation of biologically active oxidized derivatives of cholesterol as a result of its oxidation on the surface of fluorocarbon emulsions was studied. a single product of cholesterol oxidation, 7-peroxycholesterol, was found. it was shown that 7-peroxycholesterol and its derivative 7-keto-cholesterol inhibit the rosette formation between human t-lymphocytes and sheep erythrocytes. these substances exert a strong cytostatic action on the growth of procaryotic and eucaryotic cell cultures. thus, ... | 1990 | 2365071 |
| the stereospecificities of seven dehydrogenases from acholeplasma laidlawii. the simplest historical model that explains dehydrogenase stereospecificity. | stereospecificities are reported for seven dehydrogenases from acholeplasma laidlawii, an organism from an evolutionarily distinct branch of life which has not previously been studied from a stereochemical point of view. three of the activities examined (alcohol dehydrogenase, lactate dehydrogenase, and alanine dehydrogenase) catalyze the transfer of the pro-r (a) hydrogen from nadh. four other activities (3-hydroxy-3-methylglutaryl-coa reductase, glyceraldehyde-3-phosphate dehydrogenase, glucos ... | 1990 | 2365693 |
| pathogenicity of acholeplasma laidlawii for the goat udder. | 1990 | 2375734 | |
| thermotropic characterization of the 2-o-acyl,polyprenyl alpha-d-glucopyranoside isolated from palmitate-enriched acholeplasma laidlawii b membranes. | the thermotropic phase behavior of a monoacylated neutral glucolipid (2-o-acyl,polyprenyl alpha-d-glucopyranoside), isolated from palmitate-enriched acholeplasma laidlawii b membranes, was studied by differential scanning calorimetry, infrared spectroscopy and x-ray diffraction. when equilibrated at low temperatures, aqueous dispersions of this lipid form an ordered, crystal-like lamellar gel phase which transforms to an inverted hexagonal phase at temperatures near 65 degrees c upon heating. ho ... | 1990 | 2378878 |
| mycoplasmas and acholeplasmas isolated from ducks and their possible association with pasteurellas. | two hundred and sixty-three cases of clinically diseased ducks of all ages were examined for the presence of mycoplasmas. mycoplasmas and acholeplasmas belonging to more than eight serogroups were cultured from 68 of them, and comprised 12 m anatis, one m columbinasale, two m gallinaceum, two m gallinarum, nine m synoviae, three unidentified mycoplasma species, 37 acholeplasma laidlawii and one unclassified acholeplasma belonging to each of serogroups 7 and 8. they were identified by biochemical ... | 1990 | 2399638 |
| selective acylation of membrane proteins in acholeplasma laidlawii. | in membranes of the cell-wall-less prokaryote acholeplasma laidlawii most proteins are of the integral type. a substantial fraction of these proteins are enriched in hydrophilic amino acid residues. approximately 20 different major as well as minor proteins were found to be covalently modified with acyl chains. the same set of proteins are acylated when cells are grown in different fatty-acid-supplemented media. in individual proteins the ratio of palmitoyl/oleoyl acyl chains was 12-14 times lar ... | 1986 | 2420589 |
| phagocytosis of mycoplasma pneumoniae and acholeplasma laidlawii measured as inhibition of [3h]uridine uptake by macrophages. | many studies of the interaction between phagocytes and mycoplasmas have given controversial results. this is probably due both to the small size of the microorganisms and their ability to attach to the cell membrane, making it difficult to distinguish between adsorption and ingestion. to overcome these difficulties we took advantage of a phenomenon we noted occurring concomitantly with phase-contrast microscope-monitored phagocytosis of heat-killed c. albicans, i.e., a reduction of [3h]uridine u ... | 1986 | 2424998 |
| comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas. | studies were performed to compare three methods to detect mycoplasmal infection of cell cultures. the methods included microbiological assay by inoculation into broth and onto agar with anaerobic incubation, fluorescent dna staining by hoechst 33258, and mycoplasmal mediated cytotoxicity by 6 methylpurine deoxyriboside (6mpdr). fluorescent dna staining and 6mpdr assays were performed in an indicator cell culture system. a total of 2589 cell cultures were assayed. mycoplasmas were detected in 174 ... | 1986 | 2428802 |
| comparison of two methods for detection of mollicutes (mycoplasmatales and acholeplasmatales) in cell cultures in the netherlands. | a total of 1949 cell cultures was tested for contamination with mollicutes by cultivation on and in mycoplasma media, 25.7% of the cell cultures was positive, 243 strains of mycoplasma hyorhinis were isolated. furthermore, mainly m. arginini and m. orale were detected, less often acholeplasma laidlawii, m. fermentans and m. pneumoniae. optimal conditions for isolation were discussed. about one third of 217 hybridoma cultures and two third of 57 myeloma cultures proved to be contaminated, all wit ... | 1987 | 2444156 |
| molecular characterization of mycoplasma genitalium species-specific and cross-reactive determinants: identification of an immunodominant protein of m. genitalium. | species-specific proteins of mycoplasma genitalium as well as proteins cross-reactive with m. pneumoniae have been identified using monoclonal antibodies generated against these mycoplasmas in enzyme-linked immunosorbent assays (elisa) and western blot analyses. specificity of the antibodies was examined using m. hominis, m. orale m. salivarium and acholeplasma laidlawii. a 140-kda (kilodalton) protein of m. genitalium that appeared to be immunodominant in mice was also shown by radioimmunopreci ... | 1987 | 2444561 |
| immunological cross-reactivity of a mycoplasma pneumoniae membrane-associated protein antigen with mycoplasma genitalium and acholeplasma laidlawii. | a murine monoclonal antibody, oc2f5, reacts with a mycoplasma pneumoniae antigen with an approximate mr of 43,000. this antigen is trypsin and proteinase k sensitive and partitions in the detergent phase of a triton x-114 solution. the monoclonal antibody cross-reacts with an antigen from both mycoplasma genitalium and acholeplasma laidlawii with a similar molecular weight. this cross-reactivity should be considered in the development of m. pneumoniae antigen detection systems based on the use o ... | 1987 | 2447119 |
| evaluation of three methods for curing hybridomas from mycoplasma contamination. | hybridomas are as susceptible to mycoplasma contamination as animal cell cultures. two in vitro methods and one in vivo passage into mice were compared for efficiency of curing hybridomas from mycoplasma contaminations. four contaminated clones were treated with the combined action of 5-bromouracil (5-brura) and the hoechst 33258 followed by photosensitization. the other in vitro method involved the use of bm-cycline. the success of overcoming the mycoplasma contamination was dependent on the le ... | 1988 | 2453449 |
| [mechanism of the action of 12,13-epoxytrichothecenes on macromolecular synthesis in mollicutes]. | 1988 | 2471043 | |
| [the identification of restriction fragments containing rrna genes and construction of a gene bank of acholeplasma laidlawii pg8]. | 1988 | 2471045 | |
| development and application of monoclonal antibodies for the detection of cell infecting mycoplasmas. | two hybridoma cell lines producing monoclonal antibodies (mabs) to mycoplasmas were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with whole cell mycoplasma pneumoniae antigens. the mabs designated as myc-4 and myc-9 bound to a single m. pneumoniae protein band with approximate molecular weights of 150,000. in dot-eia both mabs reacted with ten mycoplasma spp., two serovars of ureaplasma urealyticum and acholeplasma laidlawii. the mabs have great potential ... | 1989 | 2513356 |
| inactivation of the vascular permeability-increasing activity of bradykinin by mycoplasmas. | mycoplasma pneumoniae, m. genitalium, m. fermentans, m. hominis, m. salivarium, m. orale, ureaplasma urealyticum and acholeplasma laidlawii inactivated the vascular permeability-increasing activity of bradykinin when the mixture of bradykinin and mycoplasma cells was injected after incubation at 37 degrees c for 1 h. cell components responsible for inactivation of the activity of bradykinin were found to be arginine-specific aminopeptidase and carboxypeptidase. | 1989 | 2515097 |
| studies on the purified, lipid-reconstituted (na+ + mg2+)-atpase from acholeplasma laidlawii b membranes. dependence of enzyme activity on lipid headgroup and hydrocarbon chain structure. | the purified (na+ + mg2+)-atpase from acholeplasma laidlawii b membranes was successfully reconstituted with a number of different phospho- and glycolipids, and the ability of these lipids to support the function of this enzyme was evaluated by their ability to increase the specific activity of the purified enzyme and by their ability to restore its lipid-phase state-dependent properties which were lost during purification. the incorporation of this atpase into liposomes composed of the endogeno ... | 1989 | 2526120 |
| a cation/proton antiport activity in acholeplasma laidlawii. | sealed membrane vesicles of acholeplasma laidlawii were obtained by controlled lysis of carotenoid-rich intact cells. an imposed delta ph was created by loading membrane vesicles or intact acholeplasma laidlawii cells with 0.25 m nh4cl and diluting them into 0.25 m choline chloride. the passive efflux of nh3 from the membrane vesicles or cells resulted in the creation of a delta ph (inside acid) that could be visualized by the quenching of the fluorescence of the weak base acridine orange. where ... | 1989 | 2544484 |
| release of mycoplasmavirus l1 upon transfection of acholeplasma laidlawii with homologous and heterologous viral dna. | this communication reports on the release of mycoplasmavirus l1 after infection of acholeplasma laidlawii with purified l3 virus. release also occurred after transfection with certain restriction fragments from mv-l3 and mv-l1 genomes. since circular molecules are efficiently taken up in polyethylene glycol-mediated transfection, inducing fragments were applied cloned in e. coli plasmids. release was also observed after electroporation of cells incubated with mv-l1 replicative intermediate dna a ... | 1989 | 2552957 |
| construction of the mycoplasma evolutionary tree from 5s rrna sequence data. | the 5s rrna sequences of eubacteria and mycoplasmas have been analyzed and a phylogenetic tree constructed. we determined the sequences of 5s rrna from clostridium innocuum, acholeplasma laidlawii, acholeplasma modicum, anaeroplasma bactoclasticum, anaeroplasma abactoclasticum, ureaplasma urealyticum, mycoplasma mycoides mycoides, mycoplasma pneumoniae, and mycoplasma gallisepticum. analysis of these and published sequences shows that mycoplasmas form a coherent phylogenetic group that, with c. ... | 1985 | 2579388 |
| the anaplerotic phosphoenolpyruvate carboxylase of the tricarboxylic acid cycle deficient acholeplasma laidlawii b-pg9. | phosphoenolpyruvate carboxylase (ec 4.1.1.31) (pep-c) was purified approximately 770-fold from the mollicute acholeplasma laidlawii b-pg9. the partially purified pep-c required phosphoenolpyruvate (pep) and mncl2 at ph 7.4 or mgcl2 at ph 8.6 for optimal activity. the product is oxaloacetate as detected by a malate dehydrogenase indicator system. the kma (pep variable) was 0.66 mm and the kmb (bicarbonate variable) was 1.02 mm. at low bicarbonate concentrations (0.5 mm), pep-c activity was stimul ... | 1989 | 2614382 |
| [development of specific technics for the prevention of mycoplasma infections in swine]. | four experimental series were run in 2 experiments with 44 unweaned piglets to test non-inactivated vaccine from ts-mutant m-60 of mycoplasma (m.) arginini and from attenuated strains of ch-2 m. hyorhinis, ep-29 m. hyosynoviae, m. suipneumoniae, and b-1 acholeplasma laidlawii. similar deviations of clinical and immunological parameters were recorded from piglets inoculated with the above vaccine and infected with pathogenic mycoplasma cultures. these deviations, however, were less strongly prono ... | 1989 | 2619458 |
| [detection certainty of the contamination of bull sperm with mycoplasma]. | tests for presence of mycoplasmas were conducted on 20 insemination bulls known as mycoplasma spreaders, with 5 sperm pellet batches of 20 pellets each being investigated for each animal. mycoplasma contamination was positively recorded from 83 of the above 100 batches. mycoplasma (m.) bovigenitalium, m. californicum. m. arginini, m. bovirhinis, and acholeplasma laidlawii were typed by means of indirect immunofluorescent technique, which confirmed the presence also in the gdr of mycoplasma speci ... | 1989 | 2619465 |
| [the "in vitro" effect of antibiotics on mycoplasmas under conditions of the preparation of bull sperm]. | bull sperm was experimentally contaminated with mycoplasma (m.) bovis, m. bovigenitalium, and acholeplasma laidlawii (10(5) colony-forming units/ml sperm). antibiotics were tested for their effectiveness under conditions of preparation of sperm for artificial insemination, primarily for their capability of killing all mycoplasma by the end of sperm equilibration (cooling to +4 degrees c within 5 h). mycoplasma decontamination was achievable only by means of nourseothricin and lincospectin, in a ... | 1989 | 2619467 |
| [the inactivation of mycoplasmas and bacteria in calf serum by 60co gamma rays]. | reported in this paper is the use of 60co gamma radiation to inactivate mycoplasmas in calf serum, newborn calf serum, and fetal calf serum. a dose of 3 kgy, independent of dose rate, was found to be sufficient for inactivation in the above sera of several mycoplasmas, including acholeplasma laidlawii, mycoplasma (m.) orale, m. arginini, m. hyorhinis, and m. bovis. the critical dose proved to be at 2 kgy. no difference was found to exist between the above species in susceptibility to irradiation ... | 1989 | 2619478 |
| [binding of oligodeoxyribonucleotides by mycoplasma rna polymerase related to gene promotors of various microorganisms]. | 1989 | 2663405 | |
| [optimization of physico-chemical parameters of reaction media for determining the activity of rna-polymerases of mollicutes]. | rna-polymerases of mollicutes differ considerably from certain species of bacteria in the temperature optimum of the activity manifestation. the activity of mollicute enzymes is considerably higher in the presence of manganese ions than in the presence of magnesium ions. they differ from escherichia coli transcriptase in this character but are similar to the rna-polymerase of lactic bacteria, hypothetic ancestors of acholeplasm. sensitivity of rna-polymerases to metals may be one of arguments ex ... | 1989 | 2668709 |
| the influence of membrane lipid composition and physical properties of membrane structure and function in acholeplasma laidlawii. | 1989 | 2669829 | |
| characterization of phospholipase a2 of mycoplasma species. | as phospholipases of mycoplasma species may play a role in the pathogenesis of respiratory tract and urogenital tract diseases mycoplasma mycoides and acholeplasma laidlawii were examined as to the production of phospholipase a2 (pla) and attempts were made to purify and characterize it. both species produced pla. the purified enzyme was found to be heat-labile, active at alkaline ph, revealing a single band in polyacrylamide gel electrophoresis. metal ions such as calcium and barium, increased ... | 1989 | 2691360 |
| mollicutes dna polymerases: characterization of a single enzyme from mycoplasma mycoides and ureaplasma urealyticum and of three enzymes from acholeplasma laidlawii. | the dna polymerase activity of different members of mollicutes was studied. a single dna polymerase was found in mycoplasma mycoides and ureaplasma urealyticum, type species of the genera mycoplasma and ureaplasma, and was compared with the previously described mycoplasma orale enzyme. most of their properties were comparable; an immunological relationship was demonstrated between m. orale and m. mycoides enzymes by immunoblotting. in contrast to these results, three different dna polymerases we ... | 1989 | 2694245 |
| transformation of acholeplasma laidlawii with streptococcal plasmids pva868 and pva920. | one limitation with studying mycoplasma genetics is the lack of cloning vectors. studies were undertaken to determine whether streptococcal plasmids could replicate in acholeplasma laidlawii, for the purpose of identifying potential vectors. plasmids pva868 and pva920 contain the same origin of replication and tetracycline resistance determinant. pva920 also contains an erythromycin resistance determinant not present in pva868. a. laidlawii was transformed with plasmids pva868 (13.7 kb) and pva9 ... | 1989 | 2740454 |
| nucleotide sequence of tryptophan trna gene in acholeplasma laidlawii. | 1989 | 2762159 | |
| determination of microbial genome sizes by two-dimensional denaturing gradient gel electrophoresis. | in two-dimensional denaturing gradient gel electrophoresis, dna is digested with a restriction endonuclease and the resulting dna fragments are separated as a function of size by conventional agarose gel electrophoresis. following this first dimension electrophoresis, the fragment distribution is placed at the top of a denaturing gradient slab gel and electrophoresis is carried out parallel to the gradient direction. this second dimension separation is a complex function of the base sequence of ... | 1989 | 2786189 |
| virus-like infectious agent (vlia) is a novel pathogenic mycoplasma: mycoplasma incognitus. | the newly recognized pathogenic virus-like infectious agent (vlia), originally reported in patients with aids but also known to be pathogenic in previously healthy non-aids patients and in non-human primates, was cultured in cell-free conditions using a modified sp-4 medium and classified as a member of the order mycoplasmatales, class mollicutes. the infectious microorganism is tentatively referred to as mycoplasma incognitus. m. incognitus has the unique biochemical properties of utilizing glu ... | 1989 | 2817215 |