Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| mycoplasma hyopneumoniae: interaction with other agents in pigs, and evaluation of immunogens. | mycoplasmal pneumonia of swine causes considerable economic losses in porciculture. diverse agents, such as environmental stress and infectious microorganisms, increase the possibility of infection, and the damage to pulmonary tissue when the infection is established. it is known that mycoplasma hyopneumoniae has an important role in this disease, in addition to secondary microbial agents, such as pasteurella multocida and actinobacillus pleuropneumoniae. however, the characteristics of this dis ... | 1994 | 7919820 |
| glycolipid receptors for attachment of mycoplasma hyopneumoniae to porcine respiratory ciliated cells. | glycolipid receptors for mycoplasma hyopneumoniae attachment were analyzed by using a thin-layer chromatography (tlc) overlay assay. m. hyopneumoniae bound specifically to sulfatide, globoside, and monosialoganglioside gm3. no binding to sphingomyelin, cerebroside, lactosyl ceramide, ceramide trihexoside, monosialogangliosides gm1 and gm2, disialogangliosides (gd1a, gd1b, and gd3), trisialoganglioside (gt1b), cholesterol, cholesterol sulfate, palmitic acid, tripalmitin, or cholesteryl palmitate ... | 1994 | 7927697 |
| clinical pharmacokinetic characterization of norfloxacin nicotinate in swine following systemic administration. | norfloxacin nicotinate (nfn) is a new water-soluble fluoroquinolone antibacterial agent. the in vitro activity of nfn for microorganisms isolated from swine and the pharmacokinetic properties of nfn following single intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) administration were investigated. the minimal inhibitory concentrations (mic's) of nfn for a wide range of reference (atcc-american type culture collection) microbial swine isolates, comprising 21 bacterial and 5 mycopl ... | 1994 | 7941848 |
| ciliostasis and loss of cilia induced by mycoplasma hyopneumoniae in porcine tracheal organ cultures. | in vivo- and in vitro-grown mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium. ciliostasis and loss of cilia in tracheal rings were induced by m. hyopneumoniae grown in vivo and with low-passage cultures when grown in vitro. levels of calmodulin or dehydrogenase enzymes in tracheal ring epithelium were not altered even though ciliostasis and loss of cilia induced ... | 1994 | 7960110 |
| rapid purification of antiserum against mycoplasma hyopneumoniae by an efficient absorption method. | a simple and efficient method for the removal of unwanted cross-reactive antibodies has been developed. the antiserum purification method was based on treatment of the antiserum with both sonicated extracts and boiling extracts of the escherichia coli host cells used in immunoscreening the lambda embl3 library. we have demonstrated unambiguously that through this simple treatment, the rabbit anti-mycoplasma hyopneumoniae antiserum can be effectively purified so that the amount of antibodies cros ... | 1994 | 8040565 |
| an improved enzyme linked immunosorbent assay (elisa) for the detection of porcine serum antibodies against mycoplasma hyopneumoniae. | an elisa for the detection of serum antibodies to mycoplasma hypopneumoniae in pigs and based on a 43 kda purified protein derived from the cytoplasmic membrane of m. hyopneumoniae strain j is described. this elisa (mhpp elisa) was compared with another recently described (auspharm elisa, sheldrake and romalis 1992) that is based on column-purified sonicated proteins of strain j. using sample to negative elisa ratios of 3 and 4 as cutoffs for inconclusive and positive reactors respectively (comp ... | 1994 | 8042274 |
| a blocking elisa using a monoclonal antibody for the serological detection of mycoplasma hyopneumoniae. | a blocking elisa was developed by using a monoclonal antibody (4082-05-344-18) which specifically detected an epitope on the mycoplasma hyopneumoniae 40 kda membrane protein without cross-reacting with m flocculare or m hyorhinis. the results obtained with sera from specific pathogen-free pigs inoculated with m flocculare or m hyorhinis confirmed the specificity of the assay. an immunoblotting procedure was used to characterise the antibody response of pigs experimentally infected with m hyopneu ... | 1994 | 8073186 |
| antibiotic susceptibility of mycoplasma hyopneumoniae isolated from swine. | the antibiotic susceptibility of thirty-nine strains of mycoplasma hyopneumoniae isolated from swine between 1970-1981 and 1989-1990 was investigated. from the present results, it is suggested that the susceptibility to chlortetracycline has been decreasing in japan. on the other hand, all the strains were sensitive to lincomycin, thiamphenicol and macrolides. newly developed macrolides such as tilmicosin, acetyl-isovaleryl-tylosin and mirosamycin had equal or higher activity than general macrol ... | 1994 | 8075234 |
| [tracheobronchial lavage technique using the transnasal route for the detection of mycoplasma hyopneumoniae in living non-anesthetized swine]. | tracheobronchial lavage has proven useful to research. the technique has been performed in anaesthetized swine using a catheter introduced by oral or intratracheal routes. the risk of mortality during anaesthesia is not negligible. in our trial, tracheobronchial lavages were realized after introducing a catheter into the lung via the nasal route. the analysis of the tracheobronchial fluid samples using a nonradioactive dna-specific probe showed the presence of mycoplasma hyopneumoniae, one of th ... | 1993 | 8111435 |
| the nature and detection of mycoplasmal immunogens. | mycoplasmal infections are important causes of disease in cattle, swine, sheep, goats and poultry. vaccination has been shown experimentally to induce protection against challenge with several mycoplasmas, and vaccines have been used to control naturally occurring mycoplasmal disease in swine, sheep, goats and poultry. immune responses to mycoplasmal immunogens have been determined using elisa and immunoblotting as well as other serologic techniques. however, the importance of specific immunogen ... | 1993 | 8116192 |
| interaction of mycoplasma hyopneumoniae and pasteurella multocida infections in swine. | to investigate the interaction between mycoplasma hyopneumoniae and pasteurella multocida infection, 32 pigs were randomly assigned by litter, sex, and weight to 4 treatment groups. group-1 pigs were inoculated with m hyopneumoniae and allowed to recover from m hyopneumoniae infection. group-2 pigs were vaccinated against m hyopneumoniae and then inoculated with m hyopneumoniae. group-3 pigs were inoculated with m hyopneumoniae and developed clinical signs of mycoplasmosis. group-4 pigs had neve ... | 1994 | 8125807 |
| differentiation of mycoplasma hyopneumoniae, m flocculare, and m hyorhinis on the basis of amplification of a 16s rrna gene sequence. | to differentiate mycoplasma hyopneumoniae, the cause of mycoplasmal pneumonia in pigs, from m flocculare and m hyorhinis, an assay, using the polymerase chain reaction to amplify a segment of the 16s rrna gene sequence, was developed. the assay was found to be useful for identification of field isolates, as well as for identification of laboratory-adapted strains. amplification of dna from m hyopneumoniae and m flocculare resulted in products of 200 and 400 base pairs, respectively. the dna from ... | 1994 | 8141501 |
| detection of enzootic pneumonia in pig herds using an enzyme-linked immunosorbent assay in sow colostrum. | a total of 6256 colostrum samples from 185 finnish pig health scheme herds were screened for mycoplasma hyopneumoniae antibodies, using a commercial elisa kit, between november 1989 and april 1991. most (162) of the herds were negative. in six herds, in which positive colostrum samples were found, the presence of enzootic pneumonia was confirmed by clinical and pathological findings. one herd had several seropositive sows but no clinical disease and in 16 herds occasional positive colostrum samp ... | 1994 | 8146441 |
| enhancement of the increase in intracellular calcium concentration in stimulated neutrophils by mycoplasma hyopneumoniae. | neutrophils isolated from the peripheral blood of pigs free of infection with mycoplasma hyopneumoniae were loaded with a fluorescent indicator (fura-2) for detection of cytosolic free calcium concentration. the kinetics of the intracellular calcium flux were examined after incubation with or without a pathogenic or a non-pathogenic strain of m. hyopneumoniae. the basal intracellular calcium concentration was not altered by incubation with m. hyopneumoniae. however, the relative increase in cyto ... | 1993 | 8146950 |
| microtiter plate adherence assay and receptor analogs for mycoplasma hyopneumoniae. | a microtiter plate adherence assay for mycoplasma hyopneumoniae was established by use of purified swine tracheal cilia which contained receptors for the mycoplasmas. m. hyopneumoniae bound specifically to plates coated with solubilized cilia. the binding was dependent on both the concentration of cilia and the number of mycoplasmas. dextran sulfate, heparin, chondroitin sulfate, laminin, mucin, and fucoidan significantly inhibited the binding of the mycoplasmas. the six inhibitors also disrupte ... | 1994 | 8168922 |
| adherence of mycoplasma hyopneumoniae to porcine ciliated respiratory tract cells. | adherence of mycoplasma hyopneumoniae to the mucosa of the distal portion of the respiratory tract of swine is an important initial event in development of mycoplasmal pneumonia. a suitable in vitro model of adherence would be useful for investigation of mycoplasmal and host cell factors involved in this process. we have developed an adherence assay, using suspensions of porcine respiratory tract ciliated epithelial cells and m hyopneumoniae. tracheal epithelial cells, collected by use of cytolo ... | 1993 | 8214893 |
| development of polymerase chain reaction primers to detect mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae (mhp) is the etiologic agent of mycoplasma pneumonia in swine. the purpose of this study was to develop a species-specific polymerase chain reaction (pcr)-based diagnostic reagent for the identification of mhp. based upon dna sequence analysis of a cloned fragment of mhp dna, pcr primers were constructed and tested against different strains of mhp, mycoplasma flocculare, other mycoplasma species, and non-mollicute micro-organisms which commonly inhabit the respiratory tr ... | 1993 | 8264672 |
| adaptation of the sensititre broth microdilution technique to antimicrobial susceptibility testing of mycoplasma hyopneumoniae. | a broth microdilution technique is described for determining the antimicrobial susceptibility of mycoplasma hyopneumoniae, using commercially prepared sensititre plates. twenty-five field isolates and two reference strains (j & 232), were tested against seven antimicrobials. field isolates were tested in duplicate and reference strains, four times to estimate reproducibility. ninety-seven percent of the duplicate mic results for the field isolates were in agreement, or within one log2 dilution. ... | 1993 | 8273275 |
| serum and mucosal antibody responses against mycoplasma hyopneumoniae following intraperitoneal vaccination and challenge of pigs with m hyopneumoniae. | pigs were immunised intraperitoneally when six weeks old and again at about 10 weeks old with killed mycoplasma hyopneumoniae antigen prepared in an oil adjuvant. the pigs were challenged with live m hyopneumoniae (beaufort strain) at between 11 and 15 weeks old. antigen specific antibody levels for both igg and iga classes in serum and respiratory tract secretion were monitored over time. in serum anti-m hyopneumoniae antibody was detected shortly after the second intraperitoneal vaccination an ... | 1993 | 8284503 |
| increased levels of tumor necrosis factor and interleukin 1 in bronchoalveolar lavage fluids from pigs infected with mycoplasma hyopneumoniae. | we examined the levels of tumor necrosis factor (tnf)-alpha and interleukin-1 (il-1) in bronchoalveolar lavage fluids (balf) from pigs experimentally infected with mycoplasma hyopneumoniae using biological assays with wehi-164 cells and d10.g4.1 cells, respectively. increased tnf-alpha and il-1 in balf were found in infected pigs with gross and/or microscopic lesions. a time-course study suggested tnf-alpha and il-1 to be persistently elevated in infected pigs. their presence in balf would thus ... | 1993 | 8291203 |
| aerosol vaccination of pigs against mycoplasma hyopneumoniae infection. | aerosol vaccination is used effectively to immunize poultry against newcastle disease, but to the authors' knowledge, this vaccination procedure is not well studied in other species. the efficacy of im and aerosol vaccination of pigs against mycoplasma hyopneumoniae infection was evaluated. twenty-one pigs from a mycoplasma-free herd were randomly allotted by litter and body weight into 3 groups. one group was given aerosolized phosphate-buffered saline solution (pbss) by inhalation. the second ... | 1993 | 8291766 |
| mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection. | forty-eight hysterectomy produced piglets reared in a pathogen-free environment were housed in 4 separate units. three groups were intratracheally challenged with m hyopneumoniae at 2 wk, or at 16 wk, or at 2 and 16 wk of age. the fourth group was uninfected and received broth medium. coughing started 2 weeks after the first infection but the booster infection did not induce any coughing. macroscopic lesions, typical of mycoplasmal pneumonia, were noted 1 wk post-infection. after 7-9 wk, piglets ... | 1993 | 8319010 |
| control of infections with mycoplasma hyopneumoniae in swine herds by disrupting the chain of infection, disinfection of buildings and strategic medical treatment. | a program with the aim to eliminate infections with mycoplasma hyopneumoniae was applied in 4 herds selling breeding pigs. a "fast" (1 month) variant of the program was applied in one herd selling young gilts (30 kg body weight) and in one herd selling adult breeding stock, and a "slow" (> or = 3.5 months) variant was applied in two corresponding herds. in both variants, the spread of infections was prevented by isolating the breeding stock. these animals were treated with tiamulin before being ... | 1993 | 8342364 |
| application of enzyme-linked immunosorbent assay for the surveillance of mycoplasma hyopneumoniae infection in pigs. | a monoclonal antibody-based blocking enzyme-linked immunosorbent assay (elisa) was used for serological surveillance of mycoplasma hyopneumoniae infection in pig herds. a follow-up study was conducted on "herd predictive values" previously reported for this elisa. of those herds giving positive results by this elisa, 42% were subsequently found to be infected, while 100% of herds giving negative results were uninfected. previous reports recorded positive and negative herd predictive values of 39 ... | 1993 | 8400395 |
| seroepidemiology of mycoplasmal pneumonia of swine in japan as surveyed by an enzyme-linked immunosorbent assay. | antibodies to mycoplasma hyopneumoniae were surveyed by enzyme-linked immunosorbent assay (elisa) on serum, colostrum and milk samples collected from sows and on sera of growing/finishing pigs in japan. only one of 196 specific-pathogen-free sows induced a low elisa value, while 72% of 411 sows from conventional herds were seropositive. a seropositive rate in the conventional sows gradually decreased with an increase in farrowing frequency or with age. in 3267 growing/finishing pigs ranging in a ... | 1993 | 8451831 |
| incidence of infections in pigs bred for slaughter revealed by elevated serum levels of interferon and development of antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. | pigs in three specialized fattening herds were studied with respect to some immune functions, viral infections revealed by presence of interferon-alpha in serum as well as bacterial infections indicated by antibodies to mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae. pigs from different breeders had signs of varying but low incidence of such infections. after transport to the fattening herds, viral infections were activated and spread during the first month, and a maximum of 19% of ... | 1993 | 8456565 |
| the influence of age and health status on the serum alpha 1-acid glycoprotein level of conventional and specific pathogen-free pigs. | serum alpha-1-acid glycoprotein (alpha 1ag) was measured in 212 landrace white pigs between birth and finishing age. the alpha 1ag level of healthy pigs five to ten months of age was 338 +/- 79 micrograms/ml, and the upper normal limit in mature swine has been established as 500 micrograms/ml. in both specific pathogen-free (spf) and conventional pigs, the alpha 1ag level within one day of birth was 14,263 +/- 2,393 micrograms/ml, 40 times the normal adult value, but rapidly decreased to 699 +/- ... | 1993 | 8490810 |
| arbitrarily primed pcr analysis of mycoplasma hyopneumoniae field isolates demonstrates genetic heterogeneity. | mycoplasma hyopneumoniae is the primary agent of mycoplasmal pneumonia in swine. in this study we performed an arbitrarily primed pcr (ap-pcr) analysis, in which low-stringency amplification with a single primer was used, to investigate genetic variability in m. hyopneumoniae strains and field isolates. we performed preliminary experiments to examine the efficacy of 40 different 10-mer oligonucleotides for priming an ap-pcr with m. hyopneumoniae jt (t = type strain) chromosomal dna. on the basis ... | 1996 | 8573513 |
| growth and microbial flora of nonmedicated, segregated, early weaned pigs from a commercial swine operation. | to determine whether segregated, early weaned pigs have better growth performance and different microbial flora than those pigs raised on-site. | 1996 | 8617629 |
| molecular characterization of a ribonucleotide reductase (nrdf) gene fragment of mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia. | a mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. one clone exhibited sequence homology to the prokaryotic r2 subunit of ribonucleotide reductase and was expressed as an 11-kda protein fused to beta-galactosidase. the vaccine potential of the fusion protein was assessed in pig trials. following experimental challenge with a virulent isolate of m. hyopneumoniae, gross lung pathology (mean goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, w ... | 1996 | 8641761 |
| dietary polyunsaturated fatty acids modulate responses of pigs to mycoplasma hyopneumoniae infection. | polyunsaturated fatty acids (pufa) are immunomodulators, but few studies have examined how these dietary components influence infectious respiratory disease. groups of nine pigs were fed casein and corn starch-based diets containing 10.5 g/100 g corn oil (co), linseed oil (lo), menhaden oil (mo), linseed + corn oil (lc, 1:1) and menhaden + corn oil (mc, 1:1). as a methodological control, one group of pigs (n = 15) was fed a commercial ration (control diet; c). pigs inoculated intratracheally wit ... | 1996 | 8648426 |
| phylogenetic comparative mutational analysis of the base-pairing between rnase p rna and its substrate. | we have studied the base-pairing between the 3'-terminal cca motif of a trna precursor and rnase p rna by a phylogenetic mutational comparative approach. thus, various derivatives of the escherichia coli trna(ser)su1 precursor harboring all possible substitutions at either the first or the second c of the 3'-terminal cca motif were generated. cleavage site selection on these precursors was studied using mutant variants of m1 rna, the catalytic subunit of e. coli rnase p, carrying changes at posi ... | 1996 | 8665413 |
| purification of hydrophobic integral membrane proteins from mycoplasma hyopneumoniae by reversed-phase high-performance liquid chromatography. | a general and practical approach for isolating, fractionating and purifying large quantities of outer membrane hydrophobic proteins is described as applied to membrane proteins of mycoplasma hyopneumoniae. outer membrane proteins were extracted with triton x-114 detergent and were precipitated from the detergent phase with 90% ethanol. precipitated proteins were dissolved in 65% formic acid and separated by rp-hplc using a formic acid-acetonitrile gradient. a m(r) 48 000 protein was obtained in ... | 1996 | 8673256 |
| polymerase chain reaction for mycoplasma hyopneumoniae detection in tracheobronchiolar washings from pigs. | we have used the polymerase chain reaction (pcr) to detect mycoplasma hyopneumoniae in tracheobronchiolar washings collected from experimentally infected piglets. on the basis of the published nucleotide sequence of m. hyopneumoniae i141 probe (accession number u02537), primers were chosen to produce an amplified fragment of 1561 bp. all the m. hyopneumoniae strains tested could be detected by the pcr test. dna from other mycoplasmal and bacterial species currently isolated from respiratory trac ... | 1996 | 8684372 |
| a field trial to evaluate a mycoplasma hyopneumoniae vaccine: effects on lung lesions and growth rates in swine. | a killed mycoplasma hyopneumoniae vaccine was evaluated in a single swine herd in which the farrowing barn and weaner rooms were on one mycoplasma-free farm, while the growing and finishing barn was on a separate farm on which mycoplasma was present. the study was carried out in a cohort of pigs born in a 12-week period. pigs born in 6 of the 12 wk were vaccinated and the rest were left as controls. the vaccine was administered twice at approximately 3 and 6 wk of age. carcass characteristics, l ... | 1996 | 8705975 |
| porcine reproductive and respiratory syndrome virus does not exacerbate mycoplasma hyopneumoniae infection in young pigs. | the purpose of this study was to determine if porcine reproductive and respiratory syndrome (prrs) virus infection altered the severity of acute mycoplasma hyopneumoniae (mh) infection in young pigs. twenty five, 3-week-old male pigs were randomly assigned by litter and weight to one of 3 groups. groups 1 (prrs only, n = 5) and 2 (prrs + mh, n = 10) were inoculated intranasally with prrs virus (in-5 isolate, 10(5) tcid50) and viremia in all pigs was confirmed by virus isolation from serum 3 days ... | 1996 | 8734647 |
| analysis of putative abc transporter genes in mycoplasma hyopneumoniae. | a previously described dna probe specific for mycoplasma hyopneumoniae (i-141) was fully sequenced and found to consist of 1618 bp and to contain two tandemly repeated orfs. the deduced amino acid sequence of the two orfs showed significant homologies with atp-binding cassette (abc) transporter proteins, particularly those of the eukaryotic multidrug resistance (mdr) protein family (up to 21% identity and 47% similarity). a somewhat lower homology was evident with the secretion protein hlyb of t ... | 1996 | 8757749 |
| [comparison of the use of a vaccine and sequential antibiotic treatment in a herd infected with mycoplasma hyopneumoniae]. | the purpose of this study was to compare the efficacy of mycoplasma hyopneumoniae vaccination with pulse medication in a pig herd chronically infected with mycoplasma hyopneumoniae. control groups of pigs were compared to vaccinated or treated groups. medication and vaccination induced a significant reduction of pneumonia score (46.37% and 29.8% respectively, compared to 53.85% for control groups, p < 0.05). the treatment did not induce a significant reduction of the mean range of lung lesions ( ... | 1996 | 8767886 |
| suppressive effect of bronchoalveolar lavage fluid from pigs infected with mycoplasma hyopneumoniae on chemiluminescence of porcine peripheral neutrophils. | bronchoalveolar lavage fluid (balf) from pigs experimentally infected with mycoplasma hyopneumoniae suppressed the chemiluminescence (cl) response of porcine polymorphonuclear neutrophils (pmn). the suppressive effect was significantly correlated with the concentration of prostaglandin e2 (pge2) in the balf. furthermore, purified human pge2 suppressed the cl response of porcine pmn. the increased level of pge2 following infection with m. hyopneumoniae may be responsible for the suppression of pm ... | 1996 | 8792569 |
| enzootic pneumonia in pigs. | this article reviews current knowledge concerning enzootic pneumonia. enzootic pneumonia, caused by mycoplasma hyopneumoniae and exacerbated by secondary invaders, occurs worldwide and has been known for many years. the organism, with its typical characteristics, invades the respiratory tract in several successive steps. clinical symptoms and lesion development are the result of the pathogenic capacity of mycoplasma hyopneumoniae and the defence reactions in the lung. the economic relevance of p ... | 1996 | 8903144 |
| structure and evolution of ribonuclease p rna in gram-positive bacteria. | the sequences and structures of rnase p rnas of some gram-positive bacteria, e.g. bacillus subtilis, are very different than those of other bacteria. in order to expand our understanding of the structure and evolution of rnase p rna in gram-positive bacteria, gene sequences encoding rnase p rnas from 10 additional species from this evolutionary group have been determined, doubling the number of sequences available for comparative analysis. the enlarged data set allows refinement of the secondary ... | 1996 | 8972865 |
| human prostatic carcinoma oncogene pti-1 is expressed in human tumor cell lines and prostate carcinoma patient blood samples. | rapid expression cloning and differential rna display identifies a gene, named prostate tumor inducing gene-1 (pti-1), that is differentially expressed in prostate cancer versus normal prostate and benign prostatic hypertrophy. pti-1 encodes a truncated and mutated human elongation factor 1 alpha, and its 5' untranslated region (utr) shares significant homology with the 23s rrna gene of mycoplasma hyopneumoniae. pcr with human genomic dnas, using pti-1 5' utr-specific primers, suggests that this ... | 1997 | 8988032 |
| restricted b-cell responses to microbial challenge of the respiratory tract. | the local b-cell response in the respiratory tract to infectious challenge has been analyzed in pigs and calves using two techniques: flow cytometry and antibody secreting cell (asc) probes. pneumonia in pigs caused by experimental infection with mycoplasma hyopneumoniae resulted in a 25-fold increase in the b-cell population in bal and lung parenchyma 28 days post infection. asc probes revealed that the b-cell response of immune pigs to a large challenge infection was localized to lung parenchy ... | 1996 | 8988866 |
| economic evaluation of national eradication and control strategies for mycoplasma hyopneumoniae in denmark. | 1996 | 8996888 | |
| cloning and functional analysis of the p97 swine cilium adhesin gene of mycoplasma hyopneumoniae. | colonization of the swine respiratory tract by mycoplasma hyopneumoniae is accomplished by specific binding to the cilia of the mucosal epithelial cells. previous studies have implicated a 97-kda outer membrane-associated protein, p97, that appeared to mediate this interaction. in order to further define the role of p97 in adherence to porcine cilia, the structural gene was cloned and sequenced, and the recombinant products were analyzed. monoclonal antibodies were used to identify recombinant c ... | 1997 | 9023217 |
| characterization of the gene encoding mhp1 from mycoplasma hyopneumoniae and examination of mhp1's vaccine potential. | the gene encoding mhp1, a 124 kda protein from mycoplasma hyopneumoniae, has been cloned, sequenced, and its product characterized. no significant homology to the gene or encoded polypeptide was found in the genbank, nbrf, or pir databases, though this protein appears similar to p97, a putative adhesin of m. hyopneumoniae described by zhang et al. (infect. immun. 63, 1013-1019, 1995). two repeated motifs were identified within the 3' end of the gene and encoded polypeptide. the mhp1 gene was fus ... | 1997 | 9041663 |
| identification of novel species-specific antigens of mycoplasma hyopneumoniae by preparative sds-page elisa profiling. | mycoplasma hyopneumoniae, m. hyorhinis and m. flocculare are commonly isolated from the respiratory tract of pigs and are phylogenetically related. the identification and characterization of antigens specific for m. hyopneumoniae is crucial for the development of serological reagents and for understanding the mechanisms of pathogenicity of this pathogen. protein and antigen profiles of six strains of m. hyopneumoniae, four strains of m. hyorhinis and a type strain of m. flocculare were compared ... | 1997 | 9043142 |
| mycoplasma hyopneumoniae infection in pigs: duration of the disease and evaluation of four diagnostic assays. | 200 spf pigs were infected by aerosol with mycoplasma hyopneumoniae and the development of clinical signs, serological and pathological reactions were studied. mean time to onset of coughing was 13 days. a mean delay of 9 days was observed from onset of coughing until seroconversion against m. hyopneumoniae as measured by elisa. at an individual level, the sensitivity for this elisa was estimated to 98-100% and the specificity to 93-100%. pasteurella multocida was isolated from the majority of t ... | 1997 | 9050168 |
| molecular cloning and analysis of a hsp (heat shock protein)-like 42 kda antigen gene of mycoplasma hyopneumoniae. | the recombinant clone expressing the 42 kda protein (p42) of mycoplasma hyopneumoniae in escherichia coli was analyzed. the 4.4 kb hindiii-xmal dna fragment expressing the p42 gene product encodes three orfs: p42 and p16 in the forwarding strand, p24 in the reverse strand. sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with mycoplasma genitalium hsp70 gene and 56% identities with bacillus subtilis dnak gene; p16 and p24 genes share 73% and 47% identiti ... | 1997 | 9111943 |
| oral immunization of mice with attenuated salmonella typhimurium aroa expressing a recombinant mycoplasma hyopneumoniae antigen (nrdf). | mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia, a commercially expensive respiratory disease of swine. salmonella typhimurium sl3261 was used as a live carrier of plasmid pkf1, which encodes a 15-kda recombinant m. hyopneumoniae protein. this expressed recombinant protein consists of the carboxy-terminal 11 kda of a 42-kda m. hyopneumoniae nrdf ribonucleotide reductase r2 subunit protein. rabbit anti-15-kda serum was able to inhibit the growth of viable m. hyopne ... | 1997 | 9169801 |
| swine mycoplasmoses. | mycoplasma hyopneumoniae is the primary agent of enzootic pneumonia in pigs. the lung lesions, generally observed in young pigs, are characterised by a hyperplasia of the epithelial cells and an increased perivascular and peribronchiolar accumulation of mononuclear cells. following m. hyopneumoniae infection, immune reactions are observed and resistance is induced in pigs. laboratory diagnosis is generally performed by an immunofluorescent test and by enzyme-linked immunosorbent assay. antibioti ... | 1996 | 9190026 |
| comparative in vitro activity of doxycycline and oxytetracycline against porcine respiratory pathogens. | minimum inhibitory concentrations of doxycycline and oxytetracycline were determined against 55 pasteurella multocida strains, 59 actinobacillus pleuropneumoniae strains and 26 mycoplasma hyopneumoniae strains isolated from the respiratory tract of pigs. an additional set of 76 p multocida strains isolated from pneumonic pigs was tested for their minimum inhibitory concentrations of doxycycline. the p multocida and a pleuropneumoniae strains were isolated in france and the minimum inhibitory con ... | 1997 | 9253829 |
| serum and mucosal antibody responses and protection in pigs vaccinated against mycoplasma hyopneumoniae with vaccines containing a denatured membrane antigen pool and adjuvant. | to investigate the protective efficacy of a pool of denatured membrane protein antigens of mycoplasma hyopneumoniae (j strain) in the molecular size range 70 to 85 kda (f3 antigen) in combination with adjuvants for pigs challenged with m hyopneumoniae. | 1997 | 9258425 |
| microbiology and pathology of fibrinous pericarditis in danish slaughter pigs. | the prevalence of fibrinous pericarditis detected at slaughter in danish slaughter pigs is approximately 0.02%. the microbiology and pathology of this disorder was studied through 46 field cases collected at slaughter from may 1994 to august 1995. mycoplasmas (mycoplasma hyopneumoniae, m. hyosynoviae and m. hyorhinis) were isolated from the pericardium in 38 cases and from the joints in six. m. hyopneumoniae dominated with 33 isolates from the pericardium. actinobacillus pleuropneumoniae was iso ... | 1997 | 9274146 |
| association of tumour necrosis factor and acute phase reactant changes with post arrival disease in swine. | severe weight loss in the absence of respiratory, enteric or systemic clinical disease or gross pathologic lesions is often observed when immunologically naive boars are placed in conventional health swine facilities. affected animals develop this weight loss in spite of receiving pre-entry vaccinations against common swine pathogens, such as haemophilus parasuis or mycoplasma hyopneumoniae. in many cases, the weight loss is non-responsive to long term antibiotic therapy. in order to determine t ... | 1997 | 9323928 |
| gene amplification (pcr) to detect and differentiate mycoplasmas in porcine mycoplasmal pneumonia. | the causative agent of porcine mycoplasmal pneumonia, mycoplasma hyopneumoniae, is difficult and time-consuming to isolate. serological identification using antibodies induced by the disease is confused by cross-reaction with a closely related organism, myc. flocculare. from pig lungs obtained at slaughter for meat and deemed free of acute disease, it was possible to detect by culture both myc. hyopneumoniae and myc. flocculare. this study has improved on an earlier pcr detection of dna from the ... | 1997 | 9418066 |
| in vitro susceptibilities of recent field isolates of mycoplasma hyopneumoniae and mycoplasma hyosynoviae to valnemulin (econor), tiamulin and enrofloxacin and the in vitro development of resistance to certain antimicrobial agents in mycoplasma hyopneumoniae. | the in vitro activities of valnemulin (econor) and two other antimicrobial agents were determined against recent field strains of mycoplasma hyopneumoniae and mycoplasma hyosynoviae using a broth microdilution method. valnemulin showed exceptional activity against m hyopneumoniae (mic90 0.0005 microgram ml-1) and m hyosynoviae (mic range 0.0001 microgram ml-1 to 0.00025 microgram ml-1) field strains. tiamulin was 100-fold less active (mic90 0.05 microgram ml-1) and enrofloxacin 20-fold less acti ... | 1997 | 9429250 |
| detection of mycoplasma hyopneumoniae by air sampling with a nested pcr assay. | this article describes the first successful detection of airborne mycoplasma hyopneumoniae under experimental and field conditions with a new nested pcr assay. air was sampled with polyethersulfone membranes (pore size, 0.2 micron) mounted in filter holders. filters were processed by dissolution and direct extraction of dna for pcr analysis. for the pcr, two nested pairs of oligonucleotide primers were designed by using an m. hyopneumoniae-specific dna sequence of a repeated gene segment. a nest ... | 1998 | 9464391 |
| antisense inhibition of the pti-1 oncogene reverses cancer phenotypes. | the genetic alterations and molecular events mediating human prostate cancer development and progression remain to be defined. rapid expression cloning and differential rna display detect a putative oncogene, prostate tumor-inducing gene 1 (pti-1), that is differentially expressed in human prostate (as well as breast, colon, and small cell lung) cancer cell lines, patient-derived prostate carcinomas, and blood from patients with metastatic prostate cancer. pti-1 consists of a unique 5' untransla ... | 1998 | 9465091 |
| experimental dual infection of cesarean-derived, colostrum-deprived pigs with mycoplasma hyopneumoniae and pseudorabies virus. | to determine whether pseudorabies virus (prv) infection increases the severity of pneumonia by mycoplasma hyopneumoniae, 18, 10-week-old cesarean-derived, colostrum-deprived pigs were randomly assigned to 3 groups of 6 pigs each. pigs in groups a and c were inoculated intranasally with m. hyopneumoniae at 10-week-old. at 11-week-old, pigs in groups b and c were inoculated intranasally with prv. all pigs were initially seronegative for m. hyopneumoniae and prv. three pigs of each group were eutha ... | 1998 | 9560775 |
| relationships between selected climatic factors in fattening units and their influence on the development of respiratory diseases in swine. | the correlation between climatic parameters with one another in fattening units and the influence of environmental factors on lung lesions registered at slaughter were studied in 6 integrated herds with continuous production systems. in addition, the influence of environmental parameters on the spread of mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae (serotypes 2 and 3) and on the productivity was monitored in 2 specialized fattening herds with strict batch production. the outdoor ... | 1998 | 9592945 |
| humoral immune responses to mycoplasma hyopneumoniae in sows and offspring following an outbreak of mycoplasmosis. | previously healthy sows, seropositive to mycoplasma hyopneumoniae, developed clinical signs of mycoplasmosis, as well as increasing amounts of antibodies to m. hyopneumoniae during an outbreak of the disease in a herd. during the early phase of the outbreak, young piglets (2 weeks) with maternal antibodies remained healthy while older seronegative piglets (4-7 weeks) developed the disease. the duration of the maternal antibodies to m. hyopneumoniae varied between litters and was related to the a ... | 1998 | 9646450 |
| detection of mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by pcr. | in the present investigation we developed a method for the detection of mycoplasma hyopneumoniae in bronchoalveolar lavage fluid (balf) of pigs by pcr with a primer pair flanking a dna fragment of 853 bp specific for m. hyopneumoniae. several methods were tested to eliminate the amplification inhibitors present in balfs. the best results were obtained by the extraction of the dna from the balfs. by the pcr performed with the extracted dna, 10(2) cfu of m. hyopneumoniae could be detected in 1 ml ... | 1998 | 9650949 |
| identification of a novel adhesin-like glycoprotein from mycoplasma hyopneumoniae. | this study identifies an adhesin-like glycoprotein, which was a 110 kda protein (p110) under hplc-gpc assay. this adhesin consisted of one p54 and two p28 subunits. in addition, n-glycosidase f could cleave all n-linked oligosaccharides on the p54 subunit. experimental results indicated that p110 with native conformations significantly inhibited the adherence of biotin-labeled porcine tracheal epithelial cell extracts to the intact m. hyopneumoniae cells (p < 0.01). furthermore, the biotin-label ... | 1998 | 9695283 |
| reiterated repeat region variability in the ciliary adhesin gene of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is a highly prevalent pathogen which colonizes the ciliated epithelial lining of the porcine respiratory tract. expression libraries constructed from genomic dna of the non-pathogenic strain m. hyopneumoniae j were screened with porcine hyperimmune antiserum against m. hyopneumoniae. one clone expressed a 28 kda protein which was also reactive with monospecific antiserum raised against a putative m. hyopneumoniae-specific 94 kda antigen derived from strain j. trypsin dig ... | 1998 | 9695926 |
| molecular analysis of the p97 cilium adhesin operon of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae causes an economically significant respiratory disease of swine called enzootic pneumonia. the disease process is initiated by adherence of m. hyopneumoniae to the cilia of swine respiratory epithelium through an interaction involving p97, a surface-associated protein, and cilia-specific receptors. binding specificity is associated with a repeat region located near the c-terminus of the p97 protein. further analysis of the dna sequences surrounding the p97 structural gen ... | 1998 | 9729120 |
| identification of the cilium binding epitope of the mycoplasma hyopneumoniae p97 adhesin. | mycoplasma hyopneumoniae colonizes the swine respiratory tract at the level of ciliated cells by attaching specifically to the cilium membrane. this interaction involves an adhesin called p97; the cilium binding activity of this protein was localized to the carboxy terminus, which included two repeat regions, r1 and r2 (t. hsu, s. artiushin, and f. c. minion, j. bacteriol. 179:1317-1323, 1997). to further delineate the molecular mechanisms of m. hyopneumoniae interactions with ciliated epitheliu ... | 1998 | 9746576 |
| efficacy of doxycycline in feed for the control of pneumonia caused by pasteurella multocida and mycoplasma hyopneumoniae in fattening pigs. | a multicentre, controlled, randomised and blinded study was carried out in three french pig herds to assess the efficacy of doxycycline administered in the feed for the control of pneumonia. about 20 per cent of 363 pigs from the three fattening units were diseased at the start of the study. pneumonic lesions were found on pigs examined postmortem and pasteurella multocida was isolated from the lungs of pigs in all the herds. mycoplasma hyopneumoniae infection was confirmed either by detection i ... | 1998 | 9787419 |
| the prevalence of mycoplasma hyopneumoniae in pig herds in western finland based on the demonstration of antibodies in colostrum by elisa. | swine enzootic pneumonia causes heavy economical losses in fattening herds. the aim of this study was to get an estimate of the prevalence of mycoplasma hyopneumoniae in the province of vaasa in western finland. there were 112 farrowing herds randomly chosen for a survey. farmers were asked to milk colostrum samples from every sow. owners of 22 herds did not send enough samples for a herd diagnosis. altogether 1773 samples (mean 18.1 samples/herd) were analysed in an indirect elisa for antibodie ... | 1998 | 9787495 |
| rnase p rna structure and cleavage reflect the primary structure of trna genes. | the function of rnase p rna depends on its folding in space. a majority of rnase p rnas from various bacteria show a similar secondary structure to that of escherichia coli (m1 rna). however, there are exceptions as exemplified by the rnase p rna derived from the low gc-content gram-positive bacteria bacillus subtilis and mycoplasma hyopneumoniae (hyo p rna). previous studies using m1 rna and hyo p rna suggest differences both with respect to the kinetics of cleavage as well as to cleavage site ... | 1998 | 9790839 |
| the effect of vaccination against mycoplasma hypopneumoniae in pig herds with a continuous production system. | an inactivated mycoplasma hyopneumoniae vaccine was evaluated in five pig herds clinically infected with enzootic pneumonia and practising a continuous production system in the growing/finishing unit. in each herd, a vaccinated and control group of approximately 47 pigs each were individually monitored from birth until slaughter. vaccinated pigs received the first dose at about 1 week of age and the second approximately 3 weeks later. during all production stages, an equal number of vaccinated a ... | 1998 | 9820117 |
| [results of field studies using stellamune mycoplasma vaccine in luxembourg]. | enzootic pneumonia caused by mycoplasma hyopneumoniae is responsible for major economic losses in pig herds worldwide. m. hyopneumoniae can also act as a primary agent of swine respiratory disease followed by bacterial or viral infections. vaccines have been developed to prevent enzootic pneumoniae. in this experiment, an inactivated vaccine against mycoplasma, also sold under the name stellamune mycoplasma (pfizer animal health) was used in 2 farms with a known history of m. hyopneumoniae infec ... | 1998 | 9868832 |
| mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. | an experimental model that demonstrates a mycoplasma species acting to potentiate a viral pneumonia was developed. mycoplasma hyopneumoniae, which produces a chronic, lymphohistiocytic bronchopneumonia in pigs, was found to potentiate the severity and the duration of a virus-induced pneumonia in pigs. pigs were inoculated with m. hyopneumoniae 21 days prior to, simultaneously with, or 10 days after inoculation with porcine reproductive and respiratory syndrome virus (prrsv), which induces an acu ... | 1999 | 9986823 |
| an on-farm study of the epidemiology of actinobacillus pleuropneumoniae infection in pigs as part of a vaccine efficacy trial. | thirty cohort pigs were followed from birth to slaughter to study epidemiological patterns of porcine pleuropneumonia caused by actinobacillus pleuropneumoniae. the study was conducted within a larger 380-animal study of vaccination against mycoplasma hyopneumoniae and a. pleuropneumoniae in a 340-sow farrow-to-finish piggery with 4-month weaning, operating a continuous system of intensive production in the north island of new zealand. the cohort pigs were randomly allocated into two equal group ... | 1999 | 10081784 |
| field efficacy of a combined use of mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae vaccines in growing pigs. | the effectiveness of simultaneous administration of commercial mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae vaccines was tested in an indoor commercial piggery which had experienced continuing respiratory-disease problems confirmed as due to both of these pathogens. piglets were randomly assigned in equal numbers to vaccination and control groups, and each vaccine was administered at a separate site to assigned piglets at two and four weeks of age. live weight of vaccinates immed ... | 1999 | 10081785 |
| effect of vaccination against mycoplasma hyopneumoniae in pig herds with an all-in/all-out production system. | a multi-site field study was conducted to evaluate an inactivated mycoplasma hyopneumoniae (mh) vaccine in 14 pig herds infected by mh and practising an all-in/all-out production system. in each herd, a vaccinated and control group of 250 pigs each were compared during the growing/finishing period with respect to performance parameters (major variables) and by means of clinical, serological and pathological parameters (ancillary variables). mh vaccination significantly (p < 0.05) improved daily ... | 1999 | 10195611 |
| translational infidelity and human cancer: role of the pti-1 oncogene. | several components of the eukaryotic protein synthesis apparatus have been associated with oncogenic transformation of cells. altered expression of translation elongation factor 1 alpha (ef-1 alpha), a core component of protein synthesis and closely related sequences have been linked with transformed phenotypes by several independent studies, in diverse systems. a dominant acting oncogene, prostate tumor inducing gene-1 (pti-1) has provided further evidence for this link. pti-1 appears to be a h ... | 1999 | 10216950 |
| health and growth performance of barrows reared in all-in/all-out or continuous flow facilities with or without a chlortetracycline feed additive. | to compare health and growth performance in barrows reared in all-in/all-out (aiao) or continuous flow (cf) management systems. | 1999 | 10328431 |
| application of a nested polymerase chain reaction assay to detect mycoplasma hyopneumoniae from nasal swabs. | the porcine respiratory disease complex (prdc) is an increasingly important cause of decreased swine productivity and is characterized by slow growth, decreased feed efficiency, anorexia, cough, and dyspnea. mycoplasma hyopneumoniae is among the most prevalent and important infectious agents associated with prdc. understanding of mycoplasmal pneumonia has been hindered by inadequate diagnostic methods. many of the currently available tests are relatively insensitive or nonspecific when used in a ... | 1999 | 10353356 |
| risk indicators for the seroprevalence of mycoplasma hyopneumoniae, porcine influenza viruses and aujeszky's disease virus in slaughter pigs from fattening pig herds. | epidemiological aspects of mycoplasma hyopneumoniae (mh), influenza h1n1 and h3n2 viruses, and aujeszky's disease virus (adv) were investigated in slaughter pigs from 50 fattening pig herds. herd factors as potential risk indicators for respiratory disease were obtained by means of a questionnaire. at slaughter, blood samples were collected from each herd, and the proportion of seropositive pigs per herd was assessed for each of these pathogens. the median herd-level seroprevalence of the agents ... | 1999 | 10416368 |
| detection and localization of mycoplasma hyopneumoniae dna in lungs from naturally infected pigs by in situ hybridization using a digoxigenin-labeled probe. | mycoplasma hyopneumoniae dna was detected in 20 naturally infected pigs by in situ hybridization using a nonradioactive digoxigenin-labeled dna probe. a 520-base-pair dna probe targeting a reiterative sequence of the m. hyopneumoniae genome was generated by the polymerase chain reaction. all 20 pigs infected with m. hyopneumoniae had distinct and positive hybridization signals without background staining. a strong hybridization signal was detected mainly in the luminal surface of bronchial and b ... | 1999 | 10421097 |
| amplified-fragment length polymorphism fingerprinting of mycoplasma species. | amplified-fragment length polymorphism (aflp) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. the potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including mycoplasma genitalium (n = 11), mycoplasma pneumoniae (n = 5), mycoplasma hominis (n = 5), mycoplasma hyopneumoniae (n = 9), myco plasma flocculare (n = 5), mycoplasma hyosynoviae (n = 10), and mycoplasma di ... | 1999 | 10488196 |
| evaluation of mycoplasma hyopneumoniae inactivated vaccine in pigs under field conditions. | an inactivated vaccine prepared from broth culture supernatant of mycoplasma hyopneumoniae with an aluminum adjuvant was evaluated in three herds (herd a: specific pathogen-free herd, herd b: high health status herd with no clinical signs of respiratory infection, herd c: low health status herd with serious epidemiological and economical problems). a total of 212 pigs from the three herds were divided into two groups. one group was injected twice with the vaccine at 4-week intervals and the othe ... | 1999 | 10563291 |
| infections with mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in fattening pigs. influence of piglet production systems and influence on production parameters. | two hundred and sixty-four feeder pigs from an age-segregated herd (a-pigs) and 264 feeder pigs from a continuous production system (c-pigs) were transferred into identical but separate rooms in a fattening herd employing all-in all-out production. on arrival, none of the a-pigs and 39% of the c-pigs were seropositive to mycoplasma hyopneumoniae (m. hyo). at slaughter 30% of the a-pigs and 99% of the c-pigs were seropositive to m. hyo. pigs with acute swine enzootic pneumonia (sep) at slaughter ... | 1999 | 10574070 |
| eradication of mycoplasma hyopneumoniae from infected swine herds joining the lso 2000 health class. | the study was conducted in order to determine if eradication of swine enzootic pneumoniae (sep) had succeeded with different variants of partial depopulation during the eradication programme on swine farrowing farms joining a health class, lso 2000. the farms in the health class need to be free from swine enzootic pneumoniae, swine dysentery, sarcoptic mange and atrophic rhinitis. twenty-one eradication attempts for m. hyopneumoniae were carried out using different variants based on separating a ... | 1999 | 10605141 |
| effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus (prrsv)-induced pneumonia by mycoplasma hyopneumoniae. | porcine reproductive and respiratory syndrome virus (prrsv) and mycoplasma hyopneumoniae are frequently isolated pathogens from pigs with respiratory disease. a previous study conducted in our laboratory found that infection with m. hyopneumoniae increased the duration and severity of respiratory disease induced by prrsv. the purpose of this experiment was to determine whether vaccination against m. hyopneumoniae and/or prrsv decreased the enhancement of prrsv-induced pneumonia. both m. hyopneum ... | 2000 | 10649626 |
| a tissue culture system to study respiratory ciliary epithelial adherence of selected swine mycoplasmas. | an in vitro culture system for swine tracheal epithelial cells was developed to study the adherence of swine mycoplasmas. swine tracheal epithelial cells were isolated by enzymatic digestion and cultured on microporous membranes. growth medium was placed under the membrane support to create air-liquid interface feeding resulting in the cells growing cilia and microvilli on the apical surface. two strains of mycoplasma hyopneumoniae (pathogenic strain 91-3 and non-pathogenic type strain j) and tw ... | 2000 | 10703709 |
| diagnosis and differentiation of mycoplasma hyopneumoniae and mycoplasma hyorhinis infections in pigs by pcr amplification of the p36 and p46 genes. | the genome of mycoplasma hyopneumoniae encodes several immunodominant proteins, including a cytosolic protein (p36), three membranous proteins (p46, p65, and p74), and an adhesin (p97). cross-reactions with m. flocculare and m. hyorhinis reduce the specificity of conventional serological detection methods. however, certain antigenic determinants of the p36 and p46 proteins have been shown to be specific for m. hyopneumoniae. in the present study, pairs of oligonucleotide primers were designed to ... | 2000 | 10747113 |
| r1 region of p97 mediates adherence of mycoplasma hyopneumoniae to swine cilia. | adherence of mycoplasma hyopneumoniae to the swine respiratory tract is mediated by the membrane protein p97. this protein is located on the outer membrane surface, and its role in adherence has been firmly established. the general region of p97 that mediates adherence to swine cilia is thought to be the r1 region near the carboxy terminus of the protein, but it was not clear if this region could mediate adherence to swine cilia independently of other p97 sequences. to examine this in more detai ... | 2000 | 10769015 |
| identification of the copper-zinc superoxide dismutase activity in mycoplasma hyopneumoniae. | copper-zinc superoxide dismutase (cu/znsod), a key enzyme in defense against toxic oxygen-free radicals, is widespread in eukaryotes and several species of gram-negative bacteria. the presence of this enzyme in mycoplasma hyopneumoniae (m. hyopneumoniae), the primary pathogen of mycoplasmal pneumonia in pigs, was examined since the polyclonal antibody against bovine cu/znsod was dominantly cross-reactive with the m. hyopneumoniae cu/znsod from whole cellular proteins. in situ activity staining o ... | 2000 | 10781728 |
| cytological and immunological changes in bronchoalveolar lavage fluid and histological observation of lung lesions in pigs immunized with mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate. | we have compared the cytology of bronchoalveolar lavage fluid (balf) and the pathology of lung lesions in pigs immunized with/without mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate on experimental infection. numbers of total cells, macrophages, neutrophils and lymphocytes have decreased in balf of vaccinated pigs following infection. the mean percentage of lung lesions, inflammatory cell infiltration into the airways and t cells accumulation around the bronchi ... | 2000 | 10812225 |
| a longitudinal study of serological patterns of respiratory infections in nine infected danish swine herds. | sixteen litters of seven pigs from each of nine danish farrow-to-finish herds were followed to investigate the serological patterns caused by natural infection with mycoplasma hyopneumoniae, pasteurella multocida toxin and actinobacillus pleuropneumoniae serotypes 2, 5-7, 12. in seven of the herds, pigs were followed as two separate cohorts started 4 weeks apart, and in two herds only one cohort was followed.a total of 999 pigs were included in the study. the pigs were blood sampled at weaning a ... | 2000 | 10821962 |
| lack of effect of aerial ammonia on atrophic rhinitis and pneumonia induced by mycoplasma hyopneumoniae and toxigenic pasteurella multocida. | the objective of this experimental study was to determine the effects of aerial ammonia on disease development and bacterial colonization in weaned pigs inoculated with toxigenic pasteurella multocida and mycoplasma hyopneumoniae. two groups of 10 pigs each were continuously exposed to 50 and 100 p.p.m. ammonia, respectively, and compared to a non-exposed control group of 20 pigs. following aerosol inoculation with m. hyopneumoniae at day 9, all pigs were aerosol-inoculated with toxigenic p. mul ... | 2000 | 10829570 |
| herd factors associated with the seroprevalences of four major respiratory pathogens in slaughter pigs from farrow-to-finish pig herds. | the objective of this study was to investigate sero-epidemiological aspects of mycoplasma hyopneumoniae (mh), influenza h1n1 and h3n2 viruses and aujeszky disease virus (adv) in fattening pigs from 150 randomly selected farrow-to-finish pig herds. different herd factors were examined as potential risk indicators for the percentage of pigs with antibodies against the 4 pathogens. the median within-herd seroprevalences of the pathogens were: mh 76%, h1n1 100%, h3n2 40% and adv 53%. there was a pos ... | 2000 | 10863948 |
| immunoblot assays using recombinant antigens for the detection of mycoplasma hyopneumoniae antibodies. | the 36kda l-lactate dehydrogenase (ldh) and a 29kda partial fragment of an abc transporter atp-binding protein analogue/multidrug resistance protein homologue (pr2) of mycoplasma hyopneumoniae were tested for their potential as diagnostic antigens. recombinant ldh was genetically engineered to contain six histidine residues at its c-terminal end, expressed in escherichia coli and purified to a high degree using ni(2+)-chelate affinity chromatography. a partial 262 amino acid segment representing ... | 2000 | 10865156 |
| species-specific monoclonal antibodies to escherichia coli-expressed p36 cytosolic protein of mycoplasma hyopneumoniae. | the p36 protein of mycoplasma hyopneumoniae is a cytosolic protein carrying species-specific antigenic determinants. based on the genomic sequence of the reference strain atcc 25934, primers were designed for pcr amplification of the p36-encoding gene (948 bp). these primers were shown to be specific to m. hyopneumoniae since no dna amplicons could be obtained with other mycoplasma species and pathogenic bacteria that commonly colonize the porcine respiratory tract. the amplified p36 gene was su ... | 2000 | 10882646 |
| molecular cloning and characterization of a unique 60 kda/72 kda antigen gene encoding enzyme i of the phosphoenolpyruvate: sugar phosphotransferase system (pts) of mycoplasma hyopneumoniae. | the recombinant clone expressing a 60 kda (p60) antigen was isolated from escherichia coli by screening a lambda embl3 genomic library using rabbit produced antiserum against mycoplasma hyopneumoniae. sequence analysis revealed that an interrupted (by a uga codon) open reading frame coding for a 72 kda protein (p72) may contain the p60 antigen gene. western blot analysis with an anti-p60 monospecific antibody confirmed the presence of a p72 antigen from the total protein of m. hyopneumoniae, and ... | 2000 | 10920262 |
| a nested pcr assay for the detection of mycoplasma hyopneumoniae in tracheobronchiolar washings from pigs. | a nested polymerase chain reaction (pcr) was developed for the detection of mycoplasma hyopneumoniae, the etiological agent of enzootic pneumonia, in tracheobronchiolar washings from live pigs. two nested pairs of oligonucleotide primers were designed from the sequence of a specific dna probe (i 141; accession number u02537). the primer combination was hp1/hp3 for the first step pcr while the nested primers (hp4/hp6) allowed amplification of a 706 bp fragment. all strains of m. hyopneumoniae tes ... | 2000 | 10925039 |
| [effect of the stellamune mycoplasma vaccine on growth, energy conversion, death, and medication use in fattening pigs on a pig farm chronically infected with mycoplasma hyopneumoniae]. | the effect of stellamune mycoplasma vaccine, administered to piglets aged 2-15 days and then 13-15 days later, on daily weight gain, energy conversion, and use of medication was examined in fattening pigs on a chronically mycoplasma hyopneumoniae infected pig farm. half of the piglets were vaccinated and the other half acted as controls. in the study design, half of the pens in the fattening unit were allocated to vaccinated pigs; the other half to non-vaccinated pigs, pen was the experimental u ... | 2000 | 10948597 |
| field evaluation of efficacy and tolerance of a 2% marbofloxacin injectable solution for the treatment of respiratory disease in fattening pigs. | in a controlled and randomized field trial carried out in three european countries, 219 fattening pigs, from seven farms with respiratory disease problems, were treated intramuscularly for three or five days either with marbofloxacin 2 mg/kg/day, or with amoxicillin 7 mg/kg/day. pigs were monitored daily until d5 (day 5) and again at d21, and were weighed at d0 and d21. pasteurella multocida, actinobacillus pleuropneumoniae, and mycoplasma hyopneumoniae were mainly identified in the pig lungs. t ... | 2000 | 10952441 |