Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the distribution of ferritin, lactoferrin and transferrin in granulomatous lymphadenitis of bovine paratuberculosis. | immunohistochemical examination of iron-binding proteins was carried out in the formalin-fixed mesenteric lymph nodes of normal cattle and of cattle with paratuberculosis. ferritin (ft) and lactoferrin (lf) were found in the granulomas in ileal lymph nodes from six infected cattle. a weak reaction for transferrin (tf) was found in granulomas of a lymph node from one of the infected cattle. ft was found in the macrophages in the medullary sinuses of normal and infected nodes; however, the reactio ... | 1988 | 3183087 |
| igg, igm and iga in the serum of cattle naturally infected with mycobacterium paratuberculosis. | serum igg, igm and iga antibody response in 20 cattle naturally infected with mycobacterium paratuberculosis and in 15 non-infected cattle were measured by enzyme-linked immunosorbent assay. a strong igg response was detected in 16 (80%) of the infected animals. diagnostic levels of igm were detectable in all of the infected animals as well as in 8 (53%) of the non-infected animals. animals with paratuberculosis had a very weak specific serum iga response and this appears to be of little value i ... | 1988 | 3219828 |
| fecal culture of mycobacterium paratuberculosis in cattle using a centrifugation specimen processing step. | 1988 | 3232608 | |
| diagnostic value of mammalian, avian and johnin ppd tuberculins in cattle herds infected by mycobacterium paratuberculosis. | 1988 | 3254082 | |
| analysis by elisa and western blotting of antibody reactivities in cattle infected with mycobacterium paratuberculosis after absorption of serum with m phlei. | preabsorption of cattle serum with mycobacterium phlei was of value in eliminating falsely positive reactions in an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against m paratuberculosis. specific antibody titres from 16 animals naturally infected with m paratuberculosis were unaffected by absorption. analysis by western blotting indicated that a different set of antigens of m paratuberculosis were recognised by serum from falsely positive reactors compared with tha ... | 1987 | 3296062 |
| relationship between mycobacterium avium, mycobacterium paratuberculosis and "wood pigeon mycobacteria". determinations by dna-dna hybridization. | the dna-dna homology percentages obtained in this study indicate that m. avium and m. paratuberculosis belong to one species. consequently, m. paratuberculosis ought to be considered a variant of m. avium, and the following designations are proposed: mycobacterium avium, subsp. avium. mycobacterium avium, subsp. paratuberculosis. identification and classification of "wood pigeon mycobacteria" occurring in wild animals have been problematic due to their dysgonic and mycobactin-dependent growth. d ... | 1988 | 3345246 |
| role of m cells and macrophages in the entrance of mycobacterium paratuberculosis into domes of ileal peyer's patches in calves. | ligated ileal loops of calves were inoculated with live and heat-killed mycobacterium paratuberculosis and were examined by light and electron microscopy. at 5 hours after inoculation, acid-fast bacilli were in subepithelial macrophages, but not in m cells covering domes. at 20 hours, more than 50 acid-fast bacilli per cross section were in subepithelial macrophages in domes. both living and heat-killed bacilli passed into domes. addition of anti-m. paratuberculosis bovine serum to the inoculum ... | 1988 | 3363791 |
| the occurrence of infectious diseases in mixed farming of domesticated wild herbivores and livestock in kenya. ii. bacterial diseases. | the prevalence of antibodies to brucella spp., mycobacterium paratuberculosis and the mycoplasma spp. causing contagious bovine pleuropneumonia and contagious caprine pleuropneumonia was determined in various species of ruminants on a ranch in the semi-arid zone of southeastern kenya. antibody titers to brucella spp. were found in eland (taurotragus oryx), oryx (oryx beisa) and camels (camelus dromedarius). reactors were not found in buffalo (syncerus caffer), sheep (ovis aries) and goats (capra ... | 1988 | 3373635 |
| effect of glucocorticoids on cows suspected of subclinical infection with m. paratuberculosis. | glucocorticoids were administered to 10 heifers suspected of subclinical infection with mycobacterium paratuberculosis. three animals remained untreated. m. paratuberculosis was isolated from the internal organs of 2 animals after this treatment but not from any of the control group. delayed type hypersensitivity and lymphocyte reactivity towards johnin and purified protein derivates of m. avium and m. bovis were depressed. a sharp increase in total leucocyte count, due to an increase in neutrop ... | 1988 | 3376411 |
| a model for analyzing growth kinetics of a slowly growing mycobacterium sp. | this report describes a simple method for quantifying viable mycobacteria and for determining generation time. we used statistical models and computer analysis of growth curves generated for the slowly growing mycobacterium mycobacterium paratuberculosis under controlled conditions to derive a mathematical formula relating the dependent variable, growth, to the independent variables, log10 number of organisms in the inoculum (inoculum size) and incubation time. growth was measured by a radiometr ... | 1988 | 3377502 |
| the effect of different levels of iron intake on the multiplication of mycobacterium paratuberculosis in c57 and c3h mice. | two groups of c57 and c3h mice of 5 weeks of age were infected via the intraperitoneal route with 2.0 mg (wet weight) of mycobacterium paratuberculosis. these were maintained with a similar number of segregated and non-inoculated mice of the same strains under specially controlled conditions of low, medium and high iron intake. mice were killed and bled at 7 months post-infection and assessments of haematological parameters and the degree of mycobacterial granulomatous involvement of abdominal a ... | 1988 | 3381491 |
| accidental self-inoculation with mycobacterium paratuberculosis bacterin (johne's bacterin) by veterinarians in wisconsin. | we surveyed wisconsin veterinarians to assess the frequency and severity of accidental self- and other human exposure to mycobacterium paratuberculosis bacterin (johne's bacterin). of 199 veterinarians administering the bacterin to cattle, 22 reported one or more exposures, including 19 needle-stick exposures, 8 skin surface exposures, and 2 oral mucosa exposures. the mean incidence of needle sticks was 5.5/100 veterinarians/year of bacterin use or 1/1,000 doses administered. the mean total dose ... | 1988 | 3391850 |
| characterization of clinical isolates of mycobacterium paratuberculosis by dna-dna hybridization and cellular fatty acid analysis. | the dna-dna homologies obtained were more than 90 per cent for all strains examined, including the reference strains of mycobacterium paratuberculosis and mycobacterium avium. consequently, in a genetic sense, m. paratuberculosis with its variants belong to a single species which should be considered a subspecies of m. avium. the same reference strains of m. paratuberculosis and m. avium showed small but distinct differences in the cellular fatty acid patterns. the norwegian goat isolate proved ... | 1988 | 3395476 |
| nucleic acid hybridization studies of mycobactin-dependent mycobacteria. | using molecular techniques, specifically, dna-dna hybridization in solution and measurement of the thermal stabilities of hybrids, we examined the genetic relationships among mycobactin-dependent mycobacteria and between such organisms and other (mycobactin-independent) mycobacteria. the mycobactin-dependent mycobacteria examined included five strains of mycobacterium paratuberculosis, two wood pigeon isolates, and one bovine isolate that was biochemically identified as mycobacterium avium. all ... | 1988 | 3410945 |
| characterization of mycobacterium paratuberculosis by gas-liquid and thin-layer chromatography and rapid demonstration of mycobactin dependence using radiometric methods. | thirty-six mycobacterium paratuberculosis isolates of bovine, caprine, and ovine origins were evaluated by using gas-liquid chromatography (glc), thin-layer chromatography (tlc), and bactec 7h12 middlebrook tb medium (12a; johnston laboratories, inc., towson, md.) in an effort to more rapidly differentiate this group of organisms from other mycobacteria. bacterial suspensions (0.1 ml) were inoculated by syringe into 7h12 broth containing 2 micrograms of mycobactin p per ml and control broth with ... | 1987 | 3429629 |
| a technique for the purification of mycobacterium paratuberculosis from the ileal mucosa of infected cattle. | mycobacterium paratuberculosis, the causative agent of paratuberculosis, produces considerable economic loss in the cattle industry in many countries. the slow growth of m. paratuberculosis has hindered investigations of the antigenic composition of the organism and the development of species-specific antigen for serological detection of this disease. this paper describes a simple method for the isolation of large quantities of viable m. paratuberculosis from the intestinal mucosa of infected ca ... | 1986 | 3533028 |
| disseminated mycobacterium paratuberculosis infection in a cow. | a cow with chronic diarrhea and weight loss caused by localization of mycobacterium paratuberculosis in the intestinal tract (johne's disease) had gross and microscopic changes indicative of a disseminated infection. a direct association between the remote lesions and the intestinal infection was shown by isolation of m paratuberculosis from renal tissue, detection of intracellular m paratuberculosis antigen(s), using an indirect immunoperoxidase method, and by the characteristic granulomatous n ... | 1987 | 3553118 |
| mycobacterium paratuberculosis infection in a colony of stumptail macaques (macaca arctoides). | mycobacterium paratuberculosis infection was documented in a colony of stumptail macaque monkeys (macaca arctoides), with 29 (76%) of 38 monkeys infected and shedding organisms in feces. thirteen deaths have occurred during the past five years. animals without overt clinical disease were shedding as many as 2 x 10(6) colony-forming units of m. paratuberculosis/g of feces. intestinal tissues from animals dying of this disease contained up to 10(8) colony-forming units of m. paratuberculosis/g of ... | 1987 | 3559275 |
| prevalence of mycobacterium paratuberculosis in ileocecal lymph nodes of cattle culled in the united states. | during 1983 and 1984, ileocecal lymph node specimens were obtained from clinically normal cattle at 76 us department of agriculture-inspected cull cattle slaughterhouses in 32 states and puerto rico. mycobacterium paratuberculosis was isolated from the lymph nodes of 119 of 7,540 cattle. the prevalence of bovine paratuberculosis was 1.6% overall, with 2.9% in dairy culls and 0.8% in beef culls. the prevalence for females and males did not appear to differ significantly. | 1987 | 3570918 |
| ingestion and intracellular growth of mycobacterium paratuberculosis within bovine blood monocytes and monocyte-derived macrophages. | cellular immunity is thought to be of major importance in resistance to infection with mycobacterium paratuberculosis, the causative agent of johne's disease of ruminants. the results of this study clarify the influence of bovine mononuclear phagocyte maturation on the ingestion and intracellular survival of m. paratuberculosis in vitro. optimal phagocytosis of m. paratuberculosis by cultured bovine blood monocytes and monocyte-derived macrophages required the presence of 5 to 20% serum; few bac ... | 1987 | 3596802 |
| determination of genome size and dna homology between an unclassified mycobacterium species isolated from patients with crohn's disease and other mycobacteria. | the genome size of an unclassified mycobacterium species, isolated from a patient with crohn's disease, was determined by measurement of dna renaturation kinetics as 3.1 x 10(9) da. the percentage of dna homology of this organism to dna of related mycobacteria was evaluated by measurement of dna renaturation with heterologous dna. these studies supported the classification of this organism as mycobacterium paratuberculosis but failed to distinguish between m. paratuberculosis and organisms of th ... | 1987 | 3655723 |
| immunohistochemical distribution of ferritin, lactoferrin, and transferrin in granulomas of bovine paratuberculosis. | granulomatous lesions of bovine paratuberculosis contained ferritin, lactoferrin, and a small amount of transferrin, as demonstrated by the immunohistochemical method. macrophages in the normal bovine ileum did not contain lactoferrin and transferrin; however, ferritin was found in individual macrophages of peyer's patches. these results may help elucidate the relationship between intracellular growth of mycobacterium paratuberculosis and the presence of iron-binding proteins in the granulomas. | 1986 | 3699898 |
| biochemical characteristics of various strains of mycobacterium paratuberculosis. | biochemical activities of 20 wild-type strains and of 2 laboratory strains of mycobacterium paratuberculosis were evaluated. biochemical activities evaluated were growth at 30 c, 37 c, and 42 c; production of urease, niacin, pyrazinamidase, arylsulfatase, and catalase; hydrolyzation of tween 80; reduction of nitrate and tellurite; and growth in 5% nacl. antimicrobial susceptibility to thiophene-2-carboxylic acid hydrazide (10 micrograms/ml), neotetrazolium chloride (1:40,000), streptomycin (2 mi ... | 1986 | 3740613 |
| in vitro transformation of lymphocytes from blood and milk of cows with subclinical paratuberculosis. | lymphocytes from blood or milk of 12 cows were evaluated in vitro for the lymphocyte's capability to proliferate in response to mitogens (phytohemagglutinin-a, concanavalin a, and pokeweed mitogen) and to an antigen prepared from mycobacterium paratuberculosis (purified protein derivative, ppd-j). responses of 4 control cows were compared with those of 4 cows subclinically infected with m paratuberculosis and with 4 apparently noninfected herdmates. blood lymphocytes or milk lymphocytes from con ... | 1986 | 3740620 |
| a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle. | serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ... | 1986 | 3753338 |
| structure and relevance of the oligosaccharide hapten of mycobacterium avium serotype 2. | the type-specific antigen of mycobacterium avium serotype 2, the most ubiquitous of serotypes within the m. avium complex and a major cause of disseminated and localized infections, was isolated and purified. it is of the glycopeptidolipid (mycoside c) class with a characteristic oligosaccharide hapten. this was released as the oligosaccharide alditol by base-catalyzed reductive beta-elimination, and the structure was established by a combination of gas chromatography-mass spectrometry, methylat ... | 1986 | 3782019 |
| mycobacterial aetiology of crohn's disease: serologic study using common mycobacterial antigens and a species-specific glycolipid antigen from mycobacterium paratuberculosis. | crohn's disease is a granulomatous form of enteritis superficially similar to johne's disease (paratuberculosis) of ruminants. recently, a mycobacterium sp closely related to mycobacterium paratuberculosis was cultured from tissues of patients with crohn's disease suggesting that m paratuberculosis may be the aetiologic agent in some cases. in addition, greater seroreactivity to m paratuberculosis has been reported in patients with crohn's disease. in the present study, we have evaluated the ser ... | 1986 | 3792917 |
| investigation of association of mycobacteria with inflammatory bowel disease by nucleic acid hybridization. | we used dna-dna hybridization to characterize a mycobacterial isolate, strain linda, that was obtained from a patient with crohn's disease and that has been reported to cause ileitis in experimental animals. we also investigated the association of this mycobacterium with crohn's disease. our results identified mycobacterium strain linda as a strain of mycobacterium paratuberculosis, the etiologic agent of johne's disease, a disease of ruminants that has some superficial resemblance to crohn's di ... | 1987 | 3793875 |
| lipoarabinomannan and lipid-free arabinomannan antigens of mycobacterium paratuberculosis. | lipoarabinomannan (lam) and lipid-free arabinomannan (am) were prepared from mycobacterium paratuberculosis. purification of lam was done by ultracentrifugation of the phenol-water-extracted crude polysaccharide, followed by affinity and anion exchange chromatography. am was purified from the supernatant of the ultracentrifuged polysaccharide or from alkaline-extracted material by gel filtration and anion exchange chromatography. chemical analysis revealed arabinose and mannose in lam (1.4:1) an ... | 1987 | 3818096 |
| a glycolipid antigen specific to mycobacterium paratuberculosis: structure and antigenicity. | mycobacterium paratuberculosis (national animal disease center strain 18 and american type culture collection strain 19698), the causative agent of johne disease (bovine paratuberculosis), contains a major immunoreactive glycopeptidolipid (polar gpl-i) that has been isolated and characterized. (formula; see text) thus, the glycolipid antigen belongs to the polar mycoside c glycopeptidolipid family present in other mycobacterial species. the distal 2,3-di-o-methyl-alpha-l-fucopyranosyl-(1----3)-a ... | 1985 | 3858803 |
| isolation of mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective dubos medium. | to isolate mycobacterium paratuberculosis from contaminated material, a selective medium, selective dubos medium (sdubos), was developed by supplementing conventional dubos medium (cdubos) with carbenicillin, polymyxin, trimethoprim, and amphotericin b. the intestine and mesenteric lymph nodes of 1,501 goats were cultured in parallel on sdubos and cdubos after decontamination with oxalic acid. the contamination rate was reduced more than 150 times by the use of sdubos. the number of positive spe ... | 1985 | 3897274 |
| control of paratuberculosis (johne's disease) in goats by vaccination. | after several years of unsuccessful efforts to eradicate paratuberculosis in goats in norway by conventional methods such as general hygienic precautions and the isolation and slaughtering of clinically affected and serologically positive animals, a vaccination programme was initiated in 1967. the vaccine used consists of two live attenuated strains of mycobacterium paratuberculosis suspended in a mixture of liquid paraffin, olive oil and pumice powder. the vaccine may be stored at 4 degrees c f ... | 1985 | 4002570 |
| [experimental paratuberculosis: biological diagnosis in calves inoculated with strains of mycobactin-dependent mycobacteria]. | during an experiment on the pathogenicity of mycobactin-dependent mycobacteria strains for calf, the kinetics of antibody formation during infection was studied. the progress of cellular immunity was followed by examining delayed hypersensitivity using four allergens (bovine tuberculin hcsm, avian tuberculin hcsm, avian tuberculin ppd, and johnin ppd), and that of humoral immunity using complement fixation test and elisa. simultaneously, the elimination of bacilli in the faeces was examined. the ... | 1985 | 4014991 |
| characterization of mycobacterium paratuberculosis of bovine, caprine, and ovine origin by gas-liquid chromatographic analysis of fatty acids in whole-cell extracts. | the cellular fatty acid composition of mycobacterium paratuberculosis from bovine, ovine, and caprine origin (grown on herrold's egg yolk medium) was determined by gas-liquid chromatography (glc) and mass spectrometry and was compared with that of 19 other mycobacterium spp. a species-specific fatty acid of m paratuberculosis was not demonstrable. gas-liquid chromatographic analysis of cellular fatty acids was used to develop flow charts for the rapid identification of m paratuberculosis and oth ... | 1985 | 4051303 |
| isolation, identification, and structural analysis of the mycobactins of mycobacterium avium, mycobacterium intracellulare, mycobacterium scrofulaceum, and mycobacterium paratuberculosis. | methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species mycobacterium avium, mycobacterium intracellulare, and mycobacterium scrofulaceum (i.e., the mais complex of organisms). the mycobactins from these three species showed a structure that is common to the mycobactins from all the mycobacteria examined to date. however, these mycobactins were unique in that they had more than one alkyl chain. the m. scrofulaceum mycobacti ... | 1985 | 4055700 |
| comparison of the pathogenicity of mycobacterium paratuberculosis and mycobacterium spp isolated from the wood pigeon (columba palumbus-l). | the pathogenicity of 4 strains of mycobacteria isolated from wood pigeons and 2 strains of mycobacterium paratuberculosis was compared in calves, mice and chickens. three of the 4 wood pigeon strains and the 2 m. paratuberculosis strains produced clinical johne's disease in calves. all 6 strains were pathogenic for mice and the 4 wood pigeon strains were pathogenic for chickens. the strains were not agglutinated by antisera to strains of the m. avium complex and all were mycobactin-dependent. it ... | 1985 | 4067024 |
| lymphocyte blastogenesis, complement fixation, and fecal culture as diagnostic tests for paratuberculosis in north american wild ruminants and domestic sheep. | the efficacy of the lymphocyte blastogenesis and complement-fixation tests and fecal culture for detection of mycobacterium paratuberculosis infection was assessed in bighorn sheep (ovis canadensis), elk (cervus elaphus nelsoni), mule deer (odocoileus hemionus), white-tailed deer (o virginianus), bighorn x mouflon (o musimon) hybrid sheep, and domestic sheep. spontaneously infected bighorns were tested at the time of capture; experimentally infected animals were tested monthly for 12 months or p ... | 1985 | 4073642 |
| characterization of mycobacterium paratuberculosis antigenic proteins. | the characterization of a purified antigen from mycobacterium paratuberculosis, recently made commercially available for use in serodiagnosis by enzyme-linked immunosorbent assay (elisa), of paratuberculosis in cattle was described. this assay had 89% specificity and 83% sensitivity for m paratuberculosis infection. the protein/polypeptide composition of the purified antigen was compared with that of a crude protoplasmic extract of strain 18 m paratuberculosis used in the agar-gel immunodiffusio ... | 1985 | 4073656 |
| comparison of mycobacterium paratuberculosis and other mycobacteria, using standard cytochemical tests. | 1966 | 4163673 | |
| [serological studies on cattle in catchment area of kabete (kenia). 2. determination of antibodies against mycobacterium paratuberculosis, brucella, salmonella, pasteurella multocida, listeria and leptospira]. | 1973 | 4199742 | |
| diagnosis of mycobacterium bovis and mycobacterium paratuberculosis infections in cattle by in vitro lymphocyte immunostimulation. | 1974 | 4208505 | |
| antigenic activities of and antibody response to mycobacterium johnei. | 1974 | 4437643 | |
| effect of methotrexate on rabbits infected with mycobacterium paratuberculosis or dermatophilus congolensis. | 1972 | 4621817 | |
| clofazimine treatment of cows naturally infected with mycobacterium paratuberculosis. | 1973 | 4683972 | |
| effect of revaccination on mycobacterium johnei infection in sheep. | 1973 | 4727899 | |
| absence of immunogenicity of an oral vaccine against mycobacterium johnei in sheep. | 1974 | 4830991 | |
| growth and metabolic characteristics of mycobacterium paratuberculosis. | the cultural characteristics of newly isolated strains of mycobacterium paratuberculosis on a variety of media were studied. the mycobactin dependence ascribed to this species of mycobacterium was found to be easily circumvented by incorporation of 1% ferric ammonium citrate in serum or egg yolk media, but the earliest and most abundant growth occurred on serum medium that contained mycobactin and sodium pyruvate. in the presence of ferric ammonium citrate, respiration did not decrease during 5 ... | 1974 | 4859342 |
| detection of antibodies to mycobacterium johnei by immunofluorescence. | 1968 | 4870830 | |
| fluorescent antibody tests in pre-clinical experimental mycobacterium johnei infections in cattle. | 1969 | 4887134 | |
| hypersensitivity and serologic responses in cattle vaccinated with disrupted mycobacterium paratuberculosis cells and subsequently infected with mycobacterium bovis. | 1969 | 4900967 | |
| some comparative clinical and biochemical observations during the hypersensitive response in mycobacterium johnei infection and fever induced by e. coli endotoxin. | 1970 | 4915659 | |
| susceptibility of swine to mycobacterium paratuberculosis. | 1971 | 4938780 | |
| serologic and allergenic effects of 3 mycobacterium paratuberculosis antigens. | 1965 | 4956659 | |
| aminoacids from hydrolysates of mycobacterium paratuberculosis (smegmatis) and mycobacterium tuberculosis in relation to age and in the presence of cysteine and folcysteine. | 1965 | 4961606 | |
| studies of johne's disease. studies on the various antigens of mycobacterium paratuberculosis by agar-gel precipitation test. | 1967 | 4968357 | |
| [hydrosoluble vitamin k (sodium methyl-2-naphthohydroquinone dibenozyldisulfonate), growth factor allowing assurance of isolation and perenniality in culture of mycobacterium johnei (m.j.) in vitro]. | 1970 | 4994411 | |
| experimental mycobacterium paratuberculosis infection in chickens. | 1972 | 5022402 | |
| susceptibility of horses to mycobacterium paratuberculosis. | 1972 | 5081479 | |
| effect of the rimino phenazine b663 (g30320) on mycobacterium johnei infection and reinfection in sheep. i. bacteriology and hypersensitivity. | 1971 | 5091662 | |
| effect of the rimino phenazine b663 (g30320) on mycobacterium johnei infection and reinfection in sheep. ii. pathology. | 1971 | 5091663 | |
| aortic lesions in goats infected with mycobacterium johnei. | 1971 | 5135641 | |
| infection of dairy cattle with mycobacterium johnei in a partially vaccinated herd. | 1971 | 5166580 | |
| the use of fluorescent antibody techniques in the study of mycobacterium johnei infections. | 1965 | 5318430 | |
| experimental oral infection of calves with mycobacterium johnei. | 1965 | 5322919 | |
| metal complexes of mycobactin p and of desferrisideramines. | crystalline gallium mycobactin p and chromic mycobactin p have been prepared. the chromic compound, unlike other metallic complexes of mycobactin p, does not detectably exchange its metal with ferric iron; it competitively antagonizes the growth-promoting action of mycobactin p towards mycobacterium johnei. mycobactin p, desferrioxamine b and desferrichrysin form coloured 1:1 complexes with ammonium vanadate. the vanadyl complexes of the water-soluble desferrisideramines are formed in aqueous so ... | 1969 | 5378379 |
| paratuberculosis in pigs. experimental infection by oral administration of mycobacterium paratuberculosis. | 1969 | 5389952 | |
| mycobacterium paratuberculosis in reproductive organs and semen of bulls. | 1970 | 5414867 | |
| a comparative intradermal test with peptidoglycolipids (pmko) from mycobacterium avium and mycobacterium johnei. | 1970 | 5417930 | |
| the failure of the rimino phenazine b663 (g30320) to reduce the level of experimental mycobacterium johnei infections in calves. | 1970 | 5440612 | |
| in vitro studies of leucocyte migration in cattle infected with mycobacterium johnei. | 1968 | 5716379 | |
| plasma enzyme and glycoprotein changes in experimental mycobacterium johnei infection of calves. | 1969 | 5775622 | |
| mycobactin requirement for growth of mycobacterium johnei in semisolid medium. | 1969 | 5783044 | |
| electron microscopical observations on mycobacterium johnei. | 1969 | 5806710 | |
| assay of the mycobactins by measurement of the growth of mycobacterium johnei. | a method is described for the assay of mycobactin p and t by turbidimetric measurement of the growth of mycobacterium johnei. the assay agrees satisfactorily with spectroscopic determination of the iron complexes. the growth curves with mycobactins p and t differ somewhat in form. an approximate ratio of activities has been determined. | 1966 | 5965258 |
| further studies on immunity to mycobacterium johnei in sheep. relationship between hypersensitivity and host response to infection. | 1966 | 6008376 | |
| [the growth of mycobacterium paratuberculosis on nutrient media]. | 1966 | 6012056 | |
| cross-reactions between mycobacterium avium and mycobacterium paratuberculosis strains in complement fixation and gel precipitation tests. | 1984 | 6241982 | |
| indirect immunoperoxidase test for the diagnosis of paratuberculosis. | an indirect immunoperoxidase procedure for the diagnosis of paratuberculosis was described. formalin-fixed ileocecal tissue containing large numbers of mycobacterium paratuberculosis organisms was used as the source of antigen. goat anti-bovine immunoglobulin g labeled with peroxidase was used as the conjugate in the test system. the method is relatively simple to do and may prove to be valuable as a routine screening test. | 1983 | 6359983 |
| review of the occurrence of mycobactin dependence among mycobacteria species. | most mycobacteria are able to make mycobactin for themselves. but, mycobacterium paratuberculosis, m. avium atypical like wood-pigeon mycobacteria and some strains of m. avium typical for the primary isolation, lack this capacity and require mycobactin for growth in the laboratory. | 1984 | 6393849 |
| enzyme-linked immunosorbent assay for detection of bovine immunoglobulin g1 antibody to a protoplasmic antigen of mycobacterium paratuberculosis. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of antibodies to mycobacterium paratuberculosis in the sera of cattle. this assay was designed to minimize the nonspecific elisa reactions caused by immunoglobulin (ig)m by measuring only igg1 antibodies against a protoplasmic antigen from the organism. the elisa detected igg1 antibodies in the sera of 58% of cattle with positive fecal cultures for m paratuberculosis compared with detection of 45% of culture-positive an ... | 1983 | 6418039 |
| agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle. | during a 19-month period, the agar gel immunodiffusion (agid) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. thirty-three cattle were agid test-positive for paratuberculosis. twenty-eight cattle (87.5%) yielded mycobacterium paratuberculosis from bacterial culture of feces. four cattle were confirmed as having paratuberculosis on necropsy. one had a negative fecal culture but was lost to follow-up. ... | 1984 | 6430850 |
| possible role of mycobacteria in inflammatory bowel disease. ii. mycobacterial antibodies in crohn's disease. | an unclassified mycobacterium species has been isolated from two patients with crohn's disease (cd). antibodies to the unclassified mycobacteria cross-reacted with mycobacterium paratuberculosis. because of this cross-reactivity, an enzyme-linked immunosorbent assay (elisa) was used to examine the sera of inflammatory bowel disease (ibd) patients, both cd (n = 56), and ulcerative colitis (uc) (n = 34), for antibodies to m. paratuberculosis, mycobacterium kansasii, and mycobacterium tuberculosis. ... | 1984 | 6437775 |
| possible role of mycobacteria in inflammatory bowel disease. i. an unclassified mycobacterium species isolated from patients with crohn's disease. | a previously unrecognized mycobacterium species was isolated from two patients with crohn's disease. the organism is an acid-fast, mycobactin-dependent mycobacterium that has characteristics which do not conform to any of the presently recognized species. it belongs to the runyon group iii mycobacteria and is most closely related to mycobacterium paratuberculosis. animal inoculation revealed pathogenicity for mice when injected intravenously or intraperitoneally, but not for rats, guinea pigs, r ... | 1984 | 6499624 |
| characteristics of an unclassified mycobacterium species isolated from patients with crohn's disease. | the characteristics of an unclassified mycobacterium sp. isolated from three patients with crohn's disease are presented. the organism is extremely fastidious and mycobactin dependent and may require up to 18 months of incubation for primary isolation. colony morphology is rough. characteristics are unlike those of any presently defined species. the isolates produced postive niacin, catalase, and 2-week arylsulfatase reactions and were susceptible to neotetrazolium chloride (1:40,000), streptomy ... | 1984 | 6511878 |
| mycolic acid patterns of some species of mycobacterium. | representative strains of some species of mycobacterium were degraded by both acid and alkaline methanolysis. two-dimensional thin-layer chromatography was used to determine the patterns of mycolic acids and other long-chain components in these methanolysates. patterns composed of alpha-, methoxy- and ketomycolates were found in mycobacterium asiaticum, mycobacterium bovis, mycobacterium gastri, mycobacterium gordonae, mycobacterium kansasii, mycobacterium marinum and mycobacterium tuberculosis; ... | 1984 | 6517656 |
| experimental infection of some north american wild ruminants and domestic sheep with mycobacterium paratuberculosis: clinical and bacteriological findings. | mycobacterium paratuberculosis originally isolated from bighorn sheep (ovis canadensis) with spontaneous paratuberculosis was used to orally inoculate rocky mountain elk (cervus elaphus nelsoni) calves, mule deer (odocoileus hemionus) fawns, white-tailed deer (odocoileus virginianus) fawns, bighorn x mouflon (ovis musimon) hybrid lambs, and domestic lambs. all experimentally exposed animals became infected. during the first year of infection, hybrid and domestic sheep were able to control the in ... | 1983 | 6644916 |
| isolation of specific peptides from mycobacterium paratuberculosis protoplasm and their use in an enzyme-linked immunosorbent assay for the detection of paratuberculosis (johne's disease) in cattle. | an antigen was isolated from the protoplasm of mycobacterium paratuberculosis by a combination of gel filtration, ion exchange, and affinity chromatography. the purified antigen constituted 7.8% of the total protein in the protoplasm. the specificity and sensitivity of the enzyme-linked immunosorbent assay (elisa) for paratuberculosis, using the purified antigen, were evaluated with sera from 104 cattle which were examined (surveyed) for m paratuberculosis infection by fecal cultural technique. ... | 1983 | 6660610 |
| diagnosis of paratuberculosis in sheep. | paratuberculosis in sheep usually is manifested as emaciation and decreased wool production. diarrhea occurred in only 18% of affected animals. significant hematologic changes included decreased rbc count, hemoglobin level and hematocrit. necropsy revealed pallor, cachexia and serous fluid in body cavities. staining of intestinal mucosal scrapings and mesenteric lymph node impression smears for acid-fast organisms revealed bright-red clumps of mycobacterium paratuberculosis bacilli. fecal examin ... | 1984 | 6727847 |
| comparison of agar gel immunodiffusion and fecal culture for identification of goats with paratuberculosis. | a large goat herd naturally infected with mycobacterium paratuberculosis has been monitored for new infections for approximately 21/2 years, using both agar gel immunodiffusion and bacterial culture of feces. on the basis of postmortem confirmation of infection status, both methods had equal diagnostic sensitivity and specificity. the agar gel immunodiffusion test offered distinct advantages in ease of performance and rapidity of test results. it was concluded that serious consideration should b ... | 1980 | 6777351 |
| eastern white-tailed deer as a reservoir of ruminant paratuberculosis. | ten white-tailed deer were shot and killed at a connecticut farm that had a 6-year history of bovine paratuberculosis, and organs from these animals were examined for evidence of paratuberculosis. mycobacterium paratuberculosis was isolated from the cecal lymph node, terminal ileum, and ileocecal valve of 1 deer and from the cecal lymph node of another. characteristic lesions and acid-fast bacilli were not observed. it was concluded that infected deer could serve as sources of infection for dome ... | 1983 | 6826436 |
| bacteria in enteric lesions of cattle. | thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ... | 1983 | 6829137 |
| diagnosis of johne's disease in sheep by counter-immunoelectrophoresis. | the use of counter-immunoelectrophoresis (ciep) in the diagnosis of johne's disease was investigated in flocks of sheep in iran and libya. in 50 sheep with no history of paratuberculosis none had antibodies to mycobacterium paratuberculosis. animals vaccinated as neonates were either negative or had reciprocal titres (rt) of less than 4 at 18 months of age unless exposed to infection. among 105 non-vaccinated adults in an infected flock 91% had antibodies. twenty-one animals that either died or ... | 1983 | 6845428 |
| pathology of spontaneous and experimental infection of north american wild ruminants with mycobacterium paratuberculosis. | spontaneous paratuberculosis was studied in free-ranging and captive bighorn sheep (ovis canadensis), and rocky mountain goats (oreamnos americanus). lesions of paratuberculosis in these species resembled the disease in domestic sheep and goats. mycobacterium paratuberculosis cultured from bighorn sheep was used to orally infect bighorn x mouflon (ovis musimon) hybrid sheep, elk (cervus elaphus nelsoni), mule deer (odocoileus hemionus), and white-tailed deer (odocoileus virginianus). clinical pa ... | 1983 | 6879954 |
| intrauterine inoculation of mycobacterium paratuberculosis into guinea pigs and cattle. | mycobacterium paratuberculosis was inoculated intrauterinely into guinea pigs and cattle near the time of insemination to assess the effect and subsequent distribution of the organisms. guinea pigs inoculated intrauterinely with 1 of 3 concentrations of m paratuberculosis or not inoculated were caged with male guinea pigs for 10 days. guinea pigs given the largest dose had the lowest rate of pregnancy when examined. at necropsy, tissues were cultured to determine extent of infection. abortion fo ... | 1982 | 7073089 |
| an improved medium for culture of mycobacterium paratuberculosis from bovine faeces. | 1982 | 7180779 | |
| lack of intracellular degradation of mycobacterium paratuberculosis by bovine macrophages infected in vitro and in vivo: light microscopic and electron microscopic observations. | blood-derived macrophages from clinically healthy cows and cows infected with mycobacterium paratuberculosis were cultured in vitro and inoculated with heat-killed m paratuberculosis. five weeks after infection, acid-fast bacteria continued to be demonstrated in the macrophages. macrophage cultures also were infected with living m paratuberculosis and were examined by electron microscopy. four weeks after infection, intact bacteria were found in all cultures. electron microscopic examinations of ... | 1981 | 7224304 |
| isolation of mycobacteria inducing cross-reactions in the complement fixation test for johne's disease. | eight bulls which gave positive reactions in the complement fixation test for johne's disease were identified in a group of 29 bulls which had previously given only negative reactions at repeated annual tests. one reacting bull was confirmed to be infected with mycobacterium paratuberculosis at autopsy, but another reactor which was slaughtered had no visible lesions and mycobacteria were not recovered in culture. faecal samples from seven further bulls (six reactors and one non-reactor) yielded ... | 1981 | 7255925 |
| mycobacterium paratuberculosis in the semen and genital organs of a semen-donor bull. | mycobacterium paratuberculosis, the cause of johne's disease, was isolated from the feces of a donor bull in an artificial insemination stud. during isolation and observation for 21 months, the organism was recovered from all of 26 fecal samples and from 8 of 31 semen samples. at necropsy, it was isolated from the intestine and adjacent lymph nodes, lung, spleen, seminal vesicles, and prostate gland but not from the testicles. we concluded that routine fecal cultures at bull studs will reveal in ... | 1981 | 7263470 |
| comparative lymphocyte stimulation studies on whole blood from vaccinated and nonvaccinated cattle with paratuberculosis. | the lymphocyte stimulation test was evaluated in the diagnosis of bovine paratuberculosis, using whole blood samples from cattle. these samples were incubated with a purified protein derivative of mycobacterium paratuberculosis. samples from vaccinated and nonvaccinated cattle from paratuberculosis-free herds and from paratuberculous herds were used. vaccinated and nonvaccinated groups from the paratuberculous herds each included fecal culture-positive cattle, fecal culture-negative cattle, and ... | 1981 | 7271016 |
| effects of physiol and chemical factors on the viability of mycobacterium paratuberculosis. | alternative methods of storing bovine fecal specimens for delayed culture of mycobacterium paratuberculosis was studied. significant (p less than 0.05) losses of viability were sustained by storage at 23 degrees c for 10 days and by treatment with 0.03 zephiran for 4 and 10 days. losses were moderate after storage at -70 degrees c for 15 weeks but significant (p greater than 0.05) after storage at 23 degrees c for 4 days. the alternative methods were compared with the standard method of processi ... | 1981 | 7309854 |