Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [removal of microcystin-lr by lactic acid bacteria]. | the performance of lactobacillus salivarius bbe09-18, l. fermentum bbe09-29 and l. casei zhang to remove the cyanobacterial toxin microcystin-lr ( mc-lr) were determined in this study, and the key factors related to the removal efficiency were investigated to analyze the specific mechanism and provide a novel way to deal with the mc-lr contaminated foods. | 2010 | 20687336 |
| expression, purification, crystallization and preliminary x-ray crystallographic data from tkta, a transketolase from the lactic acid bacterium lactobacillus salivarius. | the enzyme transketolase from the lactic acid bacterium lactobacillus salivarius (subsp. salivarius ucc118) has been recombinantly expressed and purified using an escherichia coli expression system. purified transketolase from l. salivarius has been crystallized using the vapour-diffusion technique. the crystals belonged to the trigonal space group p3(2)21, with unit-cell parameters a=b=75.43, c=184.11 a, and showed diffraction to 2.3 a resolution. | 2010 | 20693662 |
| diversity of lactobacillus and bifidobacterium in feces of herbivores, omnivores and carnivores. | the lactobacillus and bifidobacterium population in the feces of 26 animals (16 species) were studied by culture-dependent and culture-independent techniques. lactobacilli were detected from a few herbivores, all carnivores and some omnivores. lactobacillus johnsonii, lactobacillus reuteri, lactobacillus salivarius, lactobacillus vaginalis and lactobacillus ingluviei were the most dominant lactobacilli in carnivores. these species were, however, not predominant in herbivores and omnivores. lacto ... | 2010 | 21034840 |
| protective effect of lactobacillus casei strain shirota against lethal infection with multi-drug resistant salmonella enterica serovar typhimurium dt104 in mice. | the anti-infectious activity of lactobacilli against multi-drug resistant salmonella enterica serovar typhimurium dt104 (dt104) was examined in a murine model of an opportunistic antibiotic-induced infection. | 2010 | 21059159 |
| genomic diversity of lactobacillus salivarius. | strains of lactobacillus salivarius are increasingly employed as probiotic agents for humans or animals. despite the diversity of environmental sources from which they have been isolated, the genomic diversity of l. salivarius has been poorly characterized, and the implications of this diversity for strain selection have not been examined. to tackle this, we applied comparative genomic hybridization (cgh) and multilocus sequence typing (mlst) to 33 strains derived from humans, animals, or food. ... | 2010 | 21131523 |
| fate and efficacy of lacticin 3147-producing lactococcus lactis in the mammalian gastrointestinal tract. | gastrointestinal survival of the bacteriocin-producing strain, lactococcus lactis dpc6520, was evaluated systematically in vitro and in vivo with a view to using this strain to deliver biologically active lacticin 3147, a broad-spectrum bacteriocin, to the gut. the activity of the lacticin 3147 producer was also evaluated against two clinically relevant pathogens: clostridium difficile and listeria monocytogenes. when suspended in an appropriate matrix, the lactococcal strain is capable of survi ... | 2011 | 21314706 |
| modulatory effects of lactobacillus salivarius on intestinal mucosal immunity of piglets. | recent studies have demonstrated that lactobacilli or their cell components can improve certain immune function in animals. the aim of this study is to investigate the effects of porcine lactobacilli on the intestinal mucosal immunity of piglets. neonatal piglets were used as a model and were orally administrated with lactobacillus salivarius b1 isolated from the duodenal mucosa of a healthy piglet. the feces of the piglets were collected on days 7, 14, and 21 for intestinal microflora analysis. ... | 2011 | 21365445 |
| isolation of lactobacillus salivarius 1077 (nrrl b-50053) and characterization of its bacteriocin, including the antimicrobial activity spectrum. | lactobacillus salivarius 1077 (nrrl b-50053) was isolated from poultry intestinal materials, and in vitro anti-campylobacter jejuni activity was demonstrated. the isolate was then used for bacteriocin production and its enrichment. the protein content of the cell-free supernatant from the spent medium was precipitated by ammonium sulfate and dialyzed to produce the crude antimicrobial preparation. a typical bacteriocin-like response of sensitivity to proteolytic enzymes and resistance to lysozym ... | 2011 | 21378051 |
| [ph and oxidation-reduction potential change of environment during a growth of lactic acid bacteria: effects of oxidizers and reducers]. | decrease of ph and dropping of oxidation-reduction potential have been observed during growing lactobacillus salivarius 1588 and 3823, lactobacillus acidophilus 101e, and lactococcus lactis 3690 in anaerobic conditions in medium with glucose acidification. these parameters and membrane proton permeability of bacteria (c(m)(h+)) changed in the mediums with different ph. oxidizer ferrycianide and reducer dl-dithiothreitol affected the bacterial growth and their changed h+ extrusion from the cells ... | 2011 | 21442918 |
| anti-inflammatory capacity of selected lactobacilli in experimental colitis is driven by nod2-mediated recognition of a specific peptidoglycan-derived muropeptide. | background and aims inflammatory bowel disease (ibd) has been linked to a loss of tolerance towards the resident microflora. therapeutic use of probiotics is known to be strain specific, but precise mechanisms remain unclear. the role of nod2 signalling and the protective effect of lactobacillus peptidoglycan (pgn) and derived muropeptides in experimental colitis were evaluated. methods the anti-inflammatory capacity of lactobacilli and derived bacterial compounds was evaluated using the 2,4,6-t ... | 2011 | 21471573 |
| effect of glutathione on growth of the probiotic bacterium lactobacillus reuteri. | glutathione (gsh) is an abundant nonprotein thiol that plays numerous roles within the cell. previously, we showed that lactobacillus salivarius has the capacity to mount a glutathione-mediated acid-tolerance response. in the present work we provide evidence of a requirement for gsh by lactobacillus reuteri and have studied the role of gsh during cell growth. medium supplementation with 0.5 mm gsh as the sole sulfur source enhanced cell growth, resulting in an increase in glucose consumption, an ... | 2011 | 21585317 |
| protein-carbohydrate interactions between lactobacillus salivarius and pig mucins. | adherence to the gastrointestinal tract is a key element desirable for many of the proposed beneficial health effects of probiotic bacteria. the aims of this study were to determine the levels of adhesion of 3 lactobacillus salivarius strains (lb6, lb9, and lb10) to porcine small intestine mucins and to determine whether adhesion is a function of lectin-like activities. dot and western blot assays were performed to investigate bacterial adhesion. several carbohydrates and glycoproteins were eval ... | 2011 | 21622872 |
| lactobacilli antagonize the growth, motility, and adherence of brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis. | avian intestinal spirochetosis (ais) results from the colonization of the ceca and colorectum of poultry by pathogenic brachyspira species. the number of cases of ais has increased since the 2006 european union ban on the use of antibiotic growth promoters, which, together with emerging antimicrobial resistance in brachyspira, has driven renewed interest in alternative intervention strategies. probiotics have been reported as protecting livestock against infection with common enteric pathogens, ... | 2011 | 21666022 |
| the immunomodulatory properties of viable lactobacillus salivarius ssp. salivarius cect5713 are not restricted to the large intestine. | purpose: the aim of this study was to better characterise the biological effects of lactobacillus salivarius ssp. salivarius cect5713, a probiotic with immunomodulatory properties. methods: live or dead probiotic was assayed in the tnbs model of rat colitis to determine whether viability was a requisite to exert the beneficial effects. in vitro studies were also performed in caco-2 cells to evaluate its effects on epithelial cell recovery and il-8 production. finally, the probiotic was assayed i ... | 2011 | 21688122 |
| oral microflora in infants delivered vaginally and by caesarean section. | international journal of paediatric dentistry 2011 background.ôçé early in life, vaginally delivered infants exhibit a different composition of the gut flora compared with infants delivered by caesarean section (c-section); however, it is unclear whether this also applies to the oral cavity. aim.ôçé to investigate and compare the oral microbial profile between infants delivered vaginally and by c-section. design.ôçé this is a cross-sectional case-control study. eighty-four infants delivered eith ... | 2011 | 21702851 |
| regulation of the il-10/il-12 axis in human dendritic cells with probiotic bacteria. | in this study, we have used monocyte-derived dendritic cells (dcs) to design a screening model for the selection of microorganisms with the ability to suppress dc-secreted il-12p70, a critical cytokine for the induction of t-helper cell type 1 immune responses under inflammatory conditions. by the treatment of dcs with cocktails containing tlr agonists and proinflammatory cytokines, the cells increased the secretion of the th1-promoting cytokine il-12p70. clinically used probiotics were tested f ... | 2011 | 21707779 |
| in situ analysis of multispecies biofilm formation on customized titanium surfaces. | many studies to identify surfaces that enhance the incorporation of dental implants into bone and soft-tissue have been undertaken previously. however, to succeed in the clinical situation, an implant surface must not support development of microbial biofilms with a pathogenic potential. as a first step in investigating this, we used two-species and three-species biofilm models with 16s ribosomal rna fluorescence in situ hybridization and confocal laser scanning microscopy to examine the effect ... | 2011 | 21729245 |
| oral treatment of chickens with lactobacilli influences elicitation of immune responses. | commensal microbes in the intestine are in constant interaction with host cells and play a role in shaping the immune system. lactobacillus acidophilus, lactobacillus reuteri, and lactobacillus salivarius are members of the chicken intestinal microbiota and have been shown to induce different cytokine profiles in mononuclear cells in vitro. the objective of the present study was to examine the effects of these bacteria individually or in combination on the induction of antibody- and cell-mediate ... | 2011 | 21734067 |
| genome sequence of lactobacillus salivarius gj-24, a probiotic strain isolated from healthy adult intestine. | the draft genome sequence of lactobacillus salivarius gj-24 isolated from the feces of healthy adults was determined. its properties, including milk fermentation activity and bacteriocin production, suggest its potential uses as a probiotic lactic acid bacterium and start culture for dairy products. | 2011 | 21742893 |
| identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials. | three broiler feeding trials were investigated in order to identify gut bacteria consistently linked with improvements in bird performance as measured by feed efficiency. trials were done in various geographic locations and varied in diet composition, broiler breed, and bird age. gut microbial communities were investigated using microbial profiling. eight common performance-linked operational taxonomic units (otus) were identified within both the ilea (180, 492, and 564-566) and ceca (140-142, 2 ... | 2011 | 21742925 |
| methodological issues in randomized trials assessing probiotics for periodontal treatment. | dhingra k. methodological issues in randomized trials assessing probiotics for periodontal treatment. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01399.x.©2011 john wiley & sons a/s background and objective: probiotics traditionally used in medicine field are now being used in an attempt to control and treat periodontal disease. however, the trials used to analyze the effects of probiotics have been subject to methodological criticism. the aim of this review was to assess the methodolog ... | 2011 | 21777405 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| analysis of the human intestinal epithelial cell transcriptional response to lactobacillus acidophilus, lactobacillus salivarius, bifidobacterium lactis and escherichia coli. | the complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to lactobacillus acidophilus ncfmôäó, lactobacillus salivarius ls-33, bifidobacterium animalis ... | 2010 | 21831765 |
| anti-inflammatory properties of probiotic bacteria on salmonella-induced il-8 synthesis in enterocyte-like caco-2 cells. | invasion of the gut by pathogenic salmonella leads to production of il-8 that initiates inflammatory reactions to combat the bacterium. however, its persistent production causes tissue damage and interventions that suppress il-8 production prevent tissue damage. we hypothesised that probiotics could mediate their benefits via inhibition of il-8 synthesis. caco-2 cells were infected with probiotic bifidobacterium infantis w52, lactobacillus casei w56, lactococcus lactis w58, lactobacillus acidoph ... | 2010 | 21840800 |
| Lactobacillus salivarius plus fructo-oligosaccharide is superior to fructo-oligosaccharide alone for treating children with moderate to severe atopic dermatitis: a double-blind, randomized, clinical trial of efficacy and safety. | Background Some probiotics can ameliorate childhood atopic dermatitis (AD). Prebiotics have also shown some efficacy, although when combined with probiotics as synbiotics, their efficacy may improve. Objective We compared the effects of Lactobacillus salivarius and fructo-oligosaccharide (synbiotic) with fructo-oligosaccharide alone (prebiotic) on children with moderate to severe AD. Methods We randomly assigned 60 children aged 2-14 years with moderate to severe AD [SCORing AD (SCORAD) > 25] ... | 2011 | 21895621 |
| Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. | To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 × 10(3) to 6.6 × 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus planta ... | 2011 | 21906691 |
| genome sequence of lactobacillus salivarius nias840, isolated from chicken intestine. | lactobacillus salivarius is a well-known lactic acid bacterium to which increasing attention has been paid recently for use as probiotics for humans and animals. l. salivarius nias840 was first isolated from broiler chicken feces, displaying antimicrobial activities against multidrug-resistant staphylococcus aureus and salmonella enterica serovar typhimurium. here, we report the genome sequence of l. salivarius nias840 (2,046,557 bp) including a small plasmid and two megaplasmids. | 2011 | 21914873 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| lactic acid bacteria from chicken carcasses with inhibitory activity against salmonella spp. and listeria monocytogenes. | this study was conducted to isolate psychrotrophic lactic acid bacteria (lab) from chicken carcasses with inhibitory activity against strains of salmonella spp. and listeria monocytogenes. a total of 100 broiler samples were examined for the presence of lab. ninety-two lab isolates that showed antimicrobial effects against salmonella spp. and l. monocytogenes were further analysed to examine their lab (gram-positive, catalase negative, oxidase negative) and psychrotrophic characteristics (abilit ... | 2011 | 21983108 |
| Production of Multiple Bacteriocins from a Single Locus by Gastrointestinal Strains of Lactobacillus salivarius. | Bacteriocins produced by Lactobacillus salivarius isolates derived from a gastrointestinal origin have previously demonstrated efficacy for in vivo protection against Listeria monocytogenes infection. In this study, comparative genomic analysis was employed to investigate the intraspecies diversity of seven L. salivarius isolates of human and porcine intestinal origin, based on the genome of the well-characterized bacteriocin-producing strain L. salivarius UCC118. This revealed a highly conserve ... | 2011 | 21984788 |
| in vitro assessment of the immunomodulatory effects of multispecies probiotic formulations for management of allergic diseases. | modulation of the composition of the intestinal microbiota with probiotics could possibly offer a way of prevention or management of allergic diseases. the objective of this study was to determine the immunomodulating effects of various multispecies probiotic combinations in vitro, as preamble to application in vivo. multispecies probiotic combinations were formulated and tested for their effects on in vitro cytokine production by human mononuclear cells and were compared to products that alread ... | 2011 | 21986357 |
| carbohydrate catabolic flexibility in the mammalian intestinal commensal lactobacillus ruminis revealed by fermentation studies aligned to genome annotations. | lactobacillus ruminis is a poorly characterized member of the lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of complex carbohydrates. | 2011 | 21995520 |
| Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency. | Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 16S rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of contr ... | 2012 | 22018813 |
| pcr-based identification of eight lactobacillus species and 18 hr-hpv genotypes in fixed cervical samples of south african women at risk of hiv and bv. | vaginal lactobacilli assessed by pcr-based microarray and pcr-based genotyping of hpv in south african women at risk for hiv and bv. vaginal lactobacilli can be defined by microarray techniques in fixed cervical samples of south african women. cervical brush samples suspended in the coagulant fixative boonfix of one hundred women attending a health centre for hiv testing in south africa were available for this study. in the ndlovu medical centre in elandsdoorn, south africa, identification of 18 ... | 2011 | 22021225 |
| identification of lactobacilli residing in chicken ceca with antagonism against campylobacter. | bacteriocins produced by lactobacillus salivarius have been recently recognized as a natural means to control campylobacter and salmonella in live poultry. this finding is of relevance since campylobacter jejuni and campylobacter coli are the predominant species isolated from poultry that are associated with human campylobacteriosis. in the present work, lactic acid bacteria (lab) isolated from the cecum of twenty tunisian chickens were identified and those isolates with antagonism against campy ... | 2011 | 22069154 |
| mucoadhesive alginate/poly (l-lysine)/thiolated alginate microcapsules for oral delivery of lactobacillus salivarius 29. | in this study, thiolated alginate was synthesized by introduction of cysteine to alginate to prepare mucoadhesive alginate/poly (l-lysine)/thiolated alginate (apta) microcapsules for efficient oral delivery of lactobacillus salivarius 29 (ls29), a novel therapeutic lactobacillus strain, in vitro and in vivo. about 759 +/- 32.4 microm of cysteine per gram of alginate was introduced by estimation of ellman's reagent reaction. ls29-loaded apta microcapsules provided suitable morphology, size, and a ... | 2011 | 22103131 |
| Lactobacillus salivarius REN counteracted unfavorable 4-nitroquinoline-1-oxide-induced changes in colonic microflora of rats. | Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1-Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. s ... | 2011 | 22203549 |
| effects of lactobacillus salivarius ls01 (dsm 22775) treatment on adult atopic dermatitis: a randomized placebo-controlled study. | atopic dermatitis (ad) is a common inflammatory skin disease characterized by xerosis, pruritus and eczema. the role of probiotics in the prevention and the treatment of ad have been extensively studied in children with controversial results while there are few studies on an adult population. the aim of this randomized, double-blind, placebo-controlled study is to evaluate the clinical efficacy of the intake of a probiotic strain (lactobacillus salivarius ls01) in the treatment of adult patients ... | 2011 | 22230409 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| effect of oral bacteria on the mechanical behavior of titanium dental implants. | this study sought to determine whether the mechanical properties of titanium dental implants changed after exposure to bacteria. | 2016 | 22299080 |
| effect of microencapsulation of lactobacillus salivarus 29 into alginate/chitosan/alginate microcapsules on viability and cytokine induction. | harsh gastric condition causes low bioavailability of probiotics when supplied orally. polymeric encapsulation has successfully protected bacteria from harsh gastric condition and ultimately increased persistency and multiplication at the targeted region. in this study, we encapsulated ls29 into aca microcapsules and characterized them. the survivability and release of ls29 from ls29-loaded aca microcapsules in sgf and sif were studied. encapsulation efficiency of ls29 in aca microcapsules was 9 ... | 2012 | 22304243 |
| development of a multispecies biofilm community by four root canal bacteria. | the development of multispecies biofilm models are needed to explain the interactions that take place in root canal biofilms during apical periodontitis. the aim of this study was to investigate the ability of 4 root canal bacteria to establish a multispecies biofilm community and to characterize the main structural, compositional, and physiological features of this community. | 2012 | 22341068 |
| divergent metabolic outcomes arising from targeted manipulation of the gut microbiota in diet-induced obesity. | the gut microbiota is an environmental regulator of fat storage and adiposity. whether the microbiota represents a realistic therapeutic target for improving metabolic health is unclear. this study explored two antimicrobial strategies for their impact on metabolic abnormalities in murine diet-induced obesity: oral vancomycin and a bacteriocin-producing probiotic (lactobacillus salivarius ucc118 bac(+)). | 2013 | 22345653 |
| isolation and characterization of lactobacillus salivarius mtc 1026 as a potential probiotic. | researchers are becoming more interested in studying probiotics at present due to their benefit to human and animal health. in this study, newly isolated strains from human feces were evaluated for probiotic properties. a total of sixty isolated strains were collected from feces and six out of sixty isolated strains could inhibit the growth of salmonella typhi and salmonella typhimurium. the strain which gave the best inhibitory effect was selected for further characterization as a probiotic str ... | 2011 | 22353742 |
| effect of lactobacillus salivarius bacteriocin abp118 on the mouse and pig intestinal microbiota. | lactobacilli are gram-positive bacteria that are a subdominant element in the human gastrointestinal microbiota, and which are commonly used in the food industry. some lactobacilli are considered probiotic, and have been associated with health benefits. however, there is very little culture-independent information on how consumed probiotic microorganisms might affect the entire intestinal microbiota. we therefore studied the impact of the administration of lactobacillus salivarius ucc118, a micr ... | 2012 | 22363561 |
| effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds. | the objective of this paper is to evaluate the effects of oil drops containing lactobacillus salivarius wb21 on periodontal health and oral microbiota producing volatile sulfur compounds (vscs). for this study, 42 subjects were randomly assigned to receive oil samples containing l. salivarius wb21 or a placebo for two weeks. oral assessment and saliva collection were performed on days 1 and 15. bacterial analysis was performed using the real-time polymerase chain reaction and terminal restrictio ... | 2012 | 22368259 |
| differential intestinal m-cell gene expression response to gut commensals. | different rates of bacterial translocation across the gut mucosa have been reported but few studies have examined translocation of commensals at the level of the gut epithelial microfold (m) cell. we used an in vitro m-cell model to quantify translocation and determine the transcriptional response of m cells to various commensal bacteria. the transport kinetics and gene expression profile of m cells in response to different bacterial strains, namely lactobacillus salivarius, escherichia coli and ... | 2012 | 22385384 |
| heat-killed lactic acid bacteria enhance immunomodulatory potential by skewing the immune response toward th1 polarization. | heat-killed lactic acid bacteria not only possess immunomodulatory functions but also provide the advantages of longer product shelf life, easier storage, and more convenient transportation. to establish appropriate heat treatments for the industrial preparation of probiotics with immunomodulatory effects, 4 different heat treatments were used to kill 11 strains of lactic acid bacteria. comparisons among the strains and with viable forms were carried out in terms of immunomodulatory activity and ... | 2011 | 22417436 |
| transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken. | the aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. thirty-seven lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. the minimum inhibitory concentration (mic) for vancomycin, tetracycline, erythromycin and chloramphenicol ... | 2012 | 22476322 |
| determination of wine microbiota using classical method, polymerase chain method and step one real-time pcr during fermentation process. | the aim of our study was the identification of grape, must and wine microbiota during the fermentation process using a classical microbiological method and real-time pcr. the changes in different groups of microorganisms were monitored in total counts of bacteria, lactobacilli and yeasts. microbiological parameters were observed during the current collection and processing of grapes in 2009. samples were taken during the fermentation process in wine enterprises and a private vineyard. during thi ... | 2012 | 22494381 |
| characterization of lactobacillus salivarius cect 5713, a strain isolated from human milk: from genotype to phenotype. | lactobacillus salivarius cect 5713, isolated from human milk, has immunomodulatory, anti-inflammatory and antiinfectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. in this work, the relationships between several genetic features of l. salivarius cect 5713 and the corresponding phenotypes were evaluated. although it contains a plasmid-encoded bacteriocin cluster, no bacteriocin biosynthesis was observed, possibly due to ... | 2012 | 22526789 |
| effect of probiotics on the treatment of children with atopic dermatitis. | atopic dermatitis, a chronic recurrent disease, is frequently encountered in clinical practice. in the last 30 years, the prevalence of atopic dermatitis has rapidly increased due to industrialization. therefore, there have been attempts in recent years to find new ways of treating and preventing atopic dermatitis. | 2012 | 22577270 |
| lactobacillus reuteri protects epidermal keratinocytes from staphylococcus aureus-induced cell death by competitive exclusion. | recent studies have suggested that the topical application of probiotic bacteria can improve skin health or combat disease. we have utilized a primary human keratinocyte culture model to investigate whether probiotic bacteria can inhibit staphylococcus aureus infection. evaluation of the candidate probiotics lactobacillus reuteri atcc 55730, lactobacillus rhamnosus ac413, and lactobacillus salivarius ucc118 demonstrated that both l. reuteri and l. rhamnosus, but not l. salivarius, reduced s. aur ... | 2012 | 22582077 |
| genome sequence of lactobacillus salivarius smxd51, a potential probiotic strain isolated from chicken cecum, showing anti-campylobacter activity. | we report the draft genome sequence of lactobacillus salivarius smxd51, isolated from the cecum of healthy chickens showing an activity against campylobacter--the food-borne pathogen that is the most common cause of gastroenteritis in the european union (eu)--and potentially interesting features for a probiotic strain, explaining our interest in it. | 2012 | 22582370 |
| genotypic characterization and safety assessment of lactic acid bacteria from indigenous african fermented food products. | indigenous fermented food products play an essential role in the diet of millions of africans. lactic acid bacteria (lab) are among the predominant microbial species in african indigenous fermented food products and are used for different applications in the food and biotechnology industries. numerous studies have described antimicrobial susceptibility profiles of lab from different parts of the world. however, there is limited information on antimicrobial resistance profiles of lab from africa. ... | 2012 | 22594449 |
| influence of adhesion and bacteriocin production by lactobacillus salivarius on the intestinal epithelial cell transcriptional response. | lactobacillus salivarius strain ucc118 is a human intestinal isolate that has been extensively studied for its potential probiotic effects in human and animal models. the objective of this study was to determine the effect of l. salivarius ucc118 on gene expression responses in the caco-2 cell line to improve understanding of how the strain might modulate intestinal epithelial cell phenotypes. exposure of caco-2 cells to ucc118 led to the induction of several human genes (tnfaip3, nfkbia, and bi ... | 2012 | 22610429 |
| comparative evaluation of three lactobacilli with strain-specific activities for rats when supplied in drinking water. | to test the in vivo benefits of three lactobacilli and to compare their different efficacies based on strain-specific activities by using rats as an experimental model, a growth-promotion and a challenge trial were conducted. the three strains, lactobacillus salivarius g1-1, lactobacillus reuteri g22-2, and lactobacillus reuteri g8-5 shared antimicrobial, bile-salt-hydrolase and amylolytic activities in vitro, respectively. in the 17 day growth-promotion trial, 48 rats were allotted to four trea ... | 2012 | 22622624 |
| beneficial lactobacilli: effects on the vaginal tract in a murine experimental model. | vaginal probiotics containing lactic acid bacteria with activity towards pathogenic microorganisms that cause urogenital tract infections have been proposed as a valid strategy for their prophylaxis and therapy. a murine experimental model was set up to evaluate the colonization capability of beneficial human lactobacilli and their effects on the mouse vaginal mucosa and innate immune cells. five lactobacillus strains were intravaginally inoculated into previously estrogenized balb/c mice. the s ... | 2012 | 22638932 |
| presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
| effects of a lactobacillus salivarius probiotic intervention on infection, cold symptom duration and severity, and mucosal immunity in endurance athletes. | the purpose of this study was to examine the effects of a probiotic supplement during 4 mo of spring training in men and women engaged in endurance-based physical activities on incidence of upper respiratory tract infections (urti) and mucosal immune markers. sixty-six highly active individuals were randomized to probiotic (n = 33) or placebo (n = 33) groups and, under double-blind procedures, received probiotic (pro: lactobacillus salivarius, 2 × 1010 bacterium colony-forming units) or placebo ... | 2012 | 22645171 |
| in vitro screening of lactobacilli isolated from chicken excreta to control salmonella enteritidis and typhimurium. | 1. the aim of this work was to select lactic acid bacteria (lab) strains from chicks and hens of egg-laying strains for potential use to control salmonellae. 2. nineteen lab strains obtained from culture collections, and 24 strains isolated from excreta of laying hens and chicks, were evaluated for inhibitory capacities against two salmonella serotypes using a "spot-the-lawn" technique and other in vitro properties that could be predictive of antimicrobial activity. 3. the size of the inhibition ... | 2012 | 22646783 |
| effect of lyophilization and storage temperature on the activity of salivaricin crl 1328, a potential bioactive ingredient of a urogenital probiotic product. | bacteriocins are antimicrobial peptides with potential applications as therapeutic agents for the treatment of microbial infections. the aim of this work was to investigate the effect of different protectors on the activity of salivaricin crl1328, a bacteriocin produced by lactobacillus salivarius crl1328, during the lyophilization process and subsequent storage at different temperatures for 18 months using statistical models. different protectors such as mannitol, tween 80, polyethylene glycol ... | 2012 | 22688238 |
| probiotics to adolescents with obesity: effects on inflammation and metabolic syndrome. | the connections between gut microbiota, energy homeostasis, and inflammation and its role in the pathogenesis of obesity-related disorders are increasingly recognized. we aimed to investigate the effect of the probiotic strain lactobacillus salivarius ls-33 on a series of biomarkers related to inflammation and the metabolic syndrome (ms) in adolescents with obesity. | 2012 | 22695039 |
| dietary inclusion of feathers affects intestinal microbiota and microbial metabolites in growing leghorn-type chickens. | feather pecking in laying hens is a serious behavioral problem that is often associated with feather eating. the intake of feathers may influence the gut microbiota and its metabolism. the aim of this study was to determine the effect of 2 different diets, with or without 5% ground feathers, on the gut microbiota and the resulting microbial fermentation products and to identify keratin-degrading bacteria in chicken digesta. one-day-old lohmann-selected leghorn chicks were divided into 3 feeding ... | 2012 | 22700493 |
| fibrinogen-binding and platelet-aggregation activities of a lactobacillus salivarius septicaemia isolate are mediated by a novel fibrinogen-binding protein. | the marketplace for probiotic foods is burgeoning, measured in billions of euro per annum. it is imperative, however, that all bacterial strains are fully assessed for human safety. the ability to bind fibrinogen is considered a potential pathogenicity trait that can lead to platelet aggregation, serious medical complications, and in some instances, death. here we examined strains from species frequently used as probiotics for their ability to bind human fibrinogen. only one strain (ccug 47825), ... | 2012 | 22724453 |
| effect of probiotics on allergic rhinitis in df, dp or dust-sensitive children: a randomized double blind controlled trial. | to study, we examined the effect of lactobacillus salivarius on the clinical symptoms and medication use among children with established allergic rhinitis (ar). | 2013 | 22728633 |
| phenotypic and genotypic characterization of thai oral streptococci, lactobacilli and pediococci. | this work was to assess the isolation and identification of thai oral bacteria. seventeen viridans group streptococci (vgs) and seventeen lactic acid bacteria from saliva were characterization based on their morphological, physiological and biochemical characteristics and the 16s rna gene sequences analysis. the gram-positive cocci in chains of viridans group comprised of six isolates (group i) were identified as streptococcus gordonii, 6 isolates (group ii) were streptococcus salivarius, 4 isol ... | 2012 | 22735847 |
| purification and genetic characterisation of the novel bacteriocin ls2 produced by the human oral strain lactobacillus salivarius bgho1. | the aim of this study was to investigate the antimicrobial potential of lactobacillus salivarius bgho1, a human oral strain with probiotic characteristics and a broad inhibitory spectrum both against gram-positive and gram-negative pathogens. here we present the bacteriocin ls2, an extremely ph- and heat-stable peptide with antilisterial activity. ls2 is a novel member of the class iid bacteriocins, unique among all currently characterised bacteriocins. it is somewhat similar to putative bacteri ... | 2012 | 22739096 |
| evaluating the efficacy of an avian-specific probiotic to reduce the colonization of campylobacter jejuni in broiler chickens. | campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by campylobacter jejuni can be considered one of the important sources of enteric infections in humans. the use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. in vitro experiments showed that enterococcus faecium, p ... | 2012 | 22802174 |
| identification of lactic acid bacteria isolated during traditional fura processing in ghana. | fura is a millet-based spontaneously fermented dumpling produced and consumed in parts of west africa, particularly nigeria, burkina faso and ghana. from eight traditional fura production sites in northern ghana, 862 lactic acid bacteria were isolated and identified to species level using a combination of genotypic and phenotypic methods including (gtg)(5)-based pcr fingerprinting and 16s rrna gene sequencing, multiplex pcr by means of reca gene sequence comparison, conventional morphological ch ... | 2012 | 22850376 |
| purification and characterization of a new bacteriocin active against campylobacter produced by lactobacillus salivarius smxd51. | strain smxd51, isolated from chicken ceca and identified as lactobacillus salivarius, produced a component that inhibits the growth of gram-positive and gram-negative bacteria and especially campylobacter jejuni. the active peptide from the cell-free supernatant of lb. salivarius smxd51 was purified in three steps: (i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase spe upti-clean cartridge using different concentrations of acetonitrile, (iii) final purificati ... | 2012 | 22850384 |
| subspecies diversity in bacteriocin production by intestinal lactobacillus salivarius strains. | a recent comparative genomic hybridization study in our laboratory revealed considerable plasticity within the bacteriocin locus of gastrointestinal strains of lactobacillus salivarius. most notably, these analyses led to the identification of two novel unmodified bacteriocins, salivaricin l and salivaricin t, produced by the neonatal isolate l. salivarius dpc6488 with immunity, regulatory and export systems analogous to those of abp118, a two-component bacteriocin produced by the well character ... | 2015 | 22892690 |
| co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 strongly enhances the intestinal mucosal immunity of piglets. | probiotics, including bacillus spp. and lactobacillus, are potential replacements for low dose in-feed antibiotics for pig. this study aimed to evaluate the effect of the co-administration of bacillus subtilis rjgp16 and lactobacillus salivarius b1 as potential probiotics to stimulate local immune responses. thirty two newborn piglets were divided into four groups and orally administrated with different combination of probiotics (none; rjgp16; b1; rjgp16 and b1) at the age of 0, 7 and 11 days. w ... | 2013 | 22901748 |
| inhibition of staphylococcus aureus adherence to caco-2 cells by lactobacilli and cell surface properties that influence attachment. | staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. the adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. in the present study, lactobacillus salivarius and lactobacillus plantarum were investigated in vitro to examine their ability to competitively exclude s. aureus. various factors involved in attachment, including bacterial status and cell conce ... | 2012 | 22922030 |
| effects of lactobacillus salivarius, lactobacillus reuteri, and pediococcus acidilactici on the nematode caenorhabditis elegans include possible antitumor activity. | this study examined the effects of three lactic acid bacteria (lab) strains on the nematode caenorhabditis elegans. lactobacillus salivarius, lactobacillus reuteri, and pediococcus acidilactici were found to inhibit the development and growth of the worm. compared to escherichia coli used as the control, l. reuteri and p. acidilactici reduced the lifespan of wild-type and short-lived daf-16 worms. on the contrary, l. salivarius extended the lifespan of daf-16 worms when used live, but reduced it ... | 2013 | 22923095 |
| probiotics reduce gut microbial translocation and improve adult atopic dermatitis. | it has been suggested that probiotics modulate atopic dermatitis (ad) progression, but no data are actually available on their mechanisms of action and on their ability to act as immunomodulators in this pathology. | 2012 | 22955355 |
| changing of fecal flora and clinical effect of l. salivarius ls01 in adults with atopic dermatitis. | to evaluate cytokine stimulation with 3 strains of lactobacillus salivarius in vitro and to assess changes in intestinal microflora and clinical improvements in adults with atopic dermatitis (ad) induced by the strain showing the best immunomodulatory features. | 2012 | 22955359 |
| mechanism of protection of transepithelial barrier function by lactobacillus salivarius: strain dependence and attenuation by bacteriocin production. | enhanced barrier function is one mechanism whereby commensals and probiotic bacteria limit translocation of foreign antigens or pathogens in the gut. however, barrier protection is not exhibited by all probiotic or commensals and the strain-specific molecules involved remain to be clarified. we evaluated the effects of 33 individual lactobacillus salivarius strains on the hydrogen peroxide (h(2)o(2))-induced barrier impairment in human epithelial caco-2 cells. these strains showed markedly diffe ... | 2012 | 22961803 |
| differential cytokine expression in t-cell subsets of chicken caecal tonsils co-cultured with three species of lactobacillus. | members of the intestinal microbiota play an important role in the development of t-cells. little is known about responses of intestinal t-cell subsets of chickens to commensal bacteria. therefore, we set out to characterise cytokine responses in t-cells after exposure to lactobacilli. caecal tonsil mononuclear cells were isolated and co-cultured with lactobacillus acidophilus, lactobacillus reuteri and lactobacillus salivarius for 12 hours. subsequently the cd4+ and cd8+ cells were fractionated ... | 2012 | 22968409 |
| identification and characterization of a bile salt hydrolase from lactobacillus salivarius for development of novel alternatives to antibiotic growth promoters. | antibiotic growth promoters (agps) have been used as feed additives to improve average body weight gain and feed efficiency in food animals for more than 5 decades. however, there is a worldwide trend to limit agp use to protect food safety and public health, which raises an urgent need to discover effective alternatives to agps. the growth-promoting effect of agps has been shown to be highly correlated with the decreased activity of intestinal bile salt hydrolase (bsh), an enzyme that is produc ... | 2012 | 23064348 |
| bugs that debugs: probiotics. | the oral cavity harbors a diverse array of bacterial species. there are more than 600 species that colonize in the oral cavity. these include a lot of organisms that are not commonly known to reside in the gastrointestinal (gi) tract and also are more familiar: lactobacillus acidophilus, lactobacillus casei, lactobacillus fermentum, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius. the balance of all these microorganisms can easily be disturbed and a prevalence of p ... | 2012 | 23066281 |
| different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. | to evaluate the effects of different combinations of probiotics on performance, egg quality, and immune response of layer hens, a trial was carried out with 1,800 white feather layer hens of the lohmann variety. the experiment was conducted by using a completely randomized design with 9 treatments, 4 replicates, and 50 hens in each replicate. compared with the control group, group f, which added a composition of heat-inactivated lactobacillus salivarius(cb) and bacillus subtilis to the diets of ... | 2012 | 23091128 |
| fructose 6-phosphate phosphoketolase activity in wild-type strains of lactobacillus, isolated from the intestinal tract of pigs. | phosphoketolases are key enzymes of the phosphoketolase pathway of heterofermentative lactic acid bacteria, which include lactobacilli. in heterofermentative lactobacilli xylulose 5-phosphate phosphoketolase (x5ppk) is the main enzyme of the phosphoketolase pathway. however, activity of fructose 6-phosphate phosphoketolase (f6ppk) has always been considered absent in lactic acid bacteria. in this study, the f6ppk activity was detected in 24 porcine wild-type strains of lactobacillus reuteri and ... | 2012 | 23101386 |
| in vitro evaluation of the probiotic potential of lactobacillus salivarius smxd51. | lactobacillus salivarius smxd51 was previously isolated from the cecum of a tunisian poultry and found to produce a bacteriocin-like substance highly active against the foodborne pathogen campylobacter jejuni. the aim of this study was to examine some probiotic properties of the strain: acid and bile tolerance, capacity of adhesion, stimulation of immune defences (il-6, il-8, il-10 and β-defensin 2), and modulation of the barrier integrity. the results showed that l. salivarius smxd51 can tolera ... | 2012 | 23122647 |
| psychoactive bacteria lactobacillus rhamnosus (jb-1) elicits rapid frequency facilitation in vagal afferents. | mounting evidence supports the influence of the gut microbiome on the local enteric nervous system and its effects on brain chemistry and relevant behavior. vagal afferents are involved in some of these effects. we previously showed that ingestion of the probiotic bacterium lactobacillus rhamnosus (jb-1) caused extensive neurochemical changes in the brain and behavior that were abrogated by prior vagotomy. because information can be transmitted to the brain via primary afferents encoded as neuro ... | 2013 | 23139216 |
| isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly irish subjects. | to isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. | 2013 | 23181509 |
| structure of ribose 5-phosphate isomerase from the probiotic bacterium lactobacillus salivarius ucc118. | the structure of ribose 5-phosphate isomerase from the probiotic bacterium lactobacillus salivarius ucc188 has been determined at 1.72 å resolution. the structure was solved by molecular replacement, which identified the functional homodimer in the asymmetric unit. despite only showing 57% sequence identity to its closest homologue, the structure adopted the typical α and β d-ribose 5-phosphate isomerase fold. comparison to other related structures revealed high homology in the active site, allo ... | 2012 | 23192019 |
| screening for lactic acid bacteria capable of inhibiting campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment. | thermotolerant campylobacter spp., specifically campylobacter jejuni and campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. the aim of this study was to identify lactic acid bacterial (lab) strains capable of inhibiting c. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch ferme ... | 2012 | 23234730 |
| potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction. | the activities and modes of probiotic action of lactic acid bacteria isolated from infant feces were investigated for alternative application in the prevention and biotherapy of colon cancer. from a total of 81 isolates of gram-positive rod and cocci bacteria obtained from healthy infants, only 15 isolates had the probiotic criteria which included growth inhibition against eight food-borne pathogens, no blood hemolysis, and tolerance to gastrointestinal tract properties such as ph 2.5 and 0.3 % ... | 2013 | 23239414 |
| antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. | previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ... | 2013 | 23332852 |
| effect of lactobacillus salivarius ls-33 on fecal microbiota in obese adolescents. | this study is a part of the clinical trials with probiotic bacterium lactobacillus salivarius ls-33 conducted in obese adolescents. previously reported clinical studies showed no effect of ls-33 consumption on the metabolic syndrome in the subject group. the aim of the study was to investigate the impact of l. salivarius ls-33 on fecal microbiota in obese adolescents. | 2013 | 23510724 |
| codon optimisation to improve expression of a mycobacterium avium ssp. paratuberculosis-specific membrane-associated antigen by lactobacillus salivarius. | subunit and dna-based vaccines against mycobacterium avium ssp. paratuberculosis (map) attempt to overcome inherent issues associated with whole-cell formulations. however, these vaccines can be hampered by poor expression of recombinant antigens from a number of disparate hosts. the high g+c content of map invariably leads to a codon bias throughout gene expression. to investigate if the codon bias affects recombinant map antigen expression, the open reading frame of a map-specific antigen mptd ... | 2013 | 23620276 |
| lactobacillus salivarius strain fdb89 induced longevity in caenorhabditis elegans by dietary restriction. | in this study, we utilized the nematode caenorhabditis elegans to assess potential life-expanding effect of lactobacillus salivarius strain fdb89 (fdb89) isolated from feces of centenarians in bama county (guangxi, china). this study showed that feeding fdb89 extended the mean life span in c. elegans by up to 11.9% compared to that of control nematodes. the reduced reproductive capacities, pharyngeal pumping rate, growth, and increased superoxide dismutase (sod) activity and xtt reduction capaci ... | 2013 | 23625218 |
| lactobacillus salivarius ren inhibits rat oral cancer induced by 4-nitroquioline 1-oxide. | despite significant advances in cancer therapy, cancer-related mobility and mortality are still rising. alternative strategies such as cancer prevention thus become essential. probiotics represent an emerging option for cancer prevention, but studies are limited to colon cancers. the efficiency of probiotics in the prevention of other cancers and the correlative mechanism remains to be explored. a novel probiotics lactobacillus salivarius ren (l. salivarius ren) was isolated from centenarians at ... | 2013 | 23658366 |
| effect of metalloporphyrins on red autofluorescence from oral bacteria. | the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ... | 2013 | 23659237 |
| potential of probiotic strains to modulate the inflammatory responses of epithelial and immune cells in vitro. | lactobacilli are important human commensal microbiota that are considered to be probiotic as they have been shown to reduce pathogenic infections and chronic inflammation. this study compared 4 strains of lactobacilli for their probiotic potential. these 4 strains showed varying capacities for adhesion and cytokine induction (interleukin [il]-8 and il-10) in different human epithelial cells, such as primary cultures of buccal cavity cells, and established cell lines derived from epithelia of the ... | 2013 | 23686123 |
| effect of immersion and inoculation in ovo of lactobacillus spp. in embryonated chicken eggs in the prevention of salmonella enteritidis after hatch. | the protection level against salmonella enteritidis was evaluated in chickens after in ovo treatment with different species of lactobacillus spp. inoculated into the air cell or by immersion in broth culture. two hundred forty embryonated eggs were distributed into 8 groups, corresponding to treatments with lactobacillus acidophilus, lactobacillus fermentum, lactobacillus salivarius, and control. on d 18 of incubation, 4 groups were inoculated with 0.1 ml of inoculum in the air cell and 4 groups ... | 2013 | 23687152 |
| targeting the microbiota to address diet-induced obesity: a time dependent challenge. | links between the gut microbiota and host metabolism have provided new perspectives on obesity. we previously showed that the link between the microbiota and fat deposition is age- and time-dependent subject to microbial adaptation to diet over time. we also demonstrated reduced weight gain in diet-induced obese (dio) mice through manipulation of the gut microbiota with vancomycin or with the bacteriocin-producing probiotic lactobacillus salivarius ucc118 (bac(+)), with metabolic improvement ach ... | 2013 | 23762426 |
| adhesive ability means inhibition activities for lactobacillus against pathogens and s-layer protein plays an important role in adhesion. | eighty-five strains of lactobacillus were isolated from the pig intestine and identified by sequencing analysis based on 16s rrna gene, from which five lactobacillus strains with high adhesive ability were selected. the inhibition ability of the five lactobacillus strains with or without s-layer proteins against adherence of escherichia coli k88 and salmonella enteritidis 50335 to caco-2 was evaluated in vitro with lactobacillus rhamnosus gg strain (lgg) as a positive control. in addition, toler ... | 2013 | 23792230 |
| effects of selected feed additives on the performance of laying hens given a diet rich in maize dried distiller's grains with solubles (ddgs). | 1. a total of 192 isa brown hens were given diets containing a high concentration of maize dried distiller's grains with solubles (ddgs) and the effect of selected feed additives on laying performance and egg quality was determined. 2. birds were allocated to 8 treatment groups with 12 replicates (cages) of two hens and were given, from week 26 to 55, iso-caloric and iso-nitrogenous experimental diets with or without a high concentration of ddgs (200 g/kg). the diet containing ddgs was not suppl ... | 2013 | 23826901 |