Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| surfactin variants mediate species-specific biofilm formation and root colonization in bacillus. | cyclic lipopeptides (clp) and especially surfactins produced by bacillus spp. trigger biofilm formation and root colonization and are crucial for biocontrol activity and systemic resistance in plants. bacillus atrophaeus 176s isolated from the moss tortella tortuosa produces the clp fengycins, iturins and surfactins, possesses antifungal activities and can protect tomato, lettuce and sugar beet against rhizoctonia solani infection. in b. atrophaeus we identified for the first time the variant su ... | 2016 | 27306252 |
| is there an infection risk when playing drums contaminated with bacillus anthracis? | this study aims to investigate the aerosol release of a bacillus anthracis spore surrogate from two different types of drums while playing, by; (i) quantifying the number of spores aerosolized during playing; (ii) investigating spore recovery from drums over long time periods, and (iii) measuring differences between (i) and (ii) for two different drums types. | 2016 | 27348508 |
| evaluation of standardized sample collection, packaging, and decontamination procedures to assess cross-contamination potential during bacillus anthracis incident response operations. | sample collection procedures and primary receptacle (sample container and bag) decontamination methods should prevent contaminant transfer between contaminated and non-contaminated surfaces and areas during bio-incident operations. cross-contamination of personnel, equipment, or sample containers may result in the exfiltration of biological agent from the exclusion (hot) zone and have unintended negative consequences on response resources, activities and outcomes. the current study was designed ... | 2016 | 27362274 |
| a pilot study on using chlorine dioxide gas for disinfection of gastrointestinal endoscopes. | this pilot study of employing chlorine dioxide (cd) gas to disinfect gastrointestinal endoscopes was conducted to meet the expectations of many endoscopy units in china for a high-efficiency and low-cost disinfectant. | 2016 | 27381729 |
| the ability of electrochemical oxidation with a bdd anode to inactivate gram-negative and gram-positive bacteria in low conductivity sulfate medium. | the disinfection of 100 ml of synthetic water containing 7 mm na2so4 with 10(6) cfu ml(-1) of either gram-negative or gram-positive bacteria has been studied by electrochemical oxidation. the electrolytic cell was a stirred tank reactor equipped with a boron-doped diamond (bdd) anode and a stainless steel cathode and the trials were performed at acidic and neutral ph, at 33.3 ma cm(-2) and 25 °c. reactive oxygen species, pre-eminently hydroxyl radicals, were efficiently produced in both media fr ... | 2016 | 27567151 |
| a simple decontamination approach using hydrogen peroxide vapour for bacillus anthracis spore inactivation. | to evaluate the use of relatively low levels of hydrogen peroxide vapour (hpv) for the inactivation of bacillus anthracis spores within an indoor environment. | 2016 | 27569380 |
| aerosol and surface deposition characteristics of two surrogates for bacillus anthracis spores. | spores of an acrystalliferous derivative of bacillus thuringiensis subsp. kurstaki, termed btcry-, are morphologically, aerodynamically, and structurally indistinguishable from bacillus anthracis spores. btcry- spores were dispersed in a large, open-ended barn together with spores of bacillus atrophaeus subsp. globigii, a historically used surrogate for bacillus anthracis spore suspensions (2 × 10(12) cfu each of b. atrophaeus subsp. globigii and btcry-) were aerosolized in each of five spray ev ... | 2016 | 27613681 |
| screening of novel bacteria for the 2,3-butanediol production. | biotechnologically produced 2,3-butanediol (2,3-bdo) is a potential starting material for industrial bulk chemicals such as butadiene or methyl ethyl ketone which are currently produced from fossil feedstocks. so far, the highest 2,3-bdo concentrations have been obtained with risk group 2 microorganisms. in this study, three risk group 1 microorganisms are presented that are so far unknown for an efficient production of 2,3-bdo. the strains bacillus atrophaeus nrs-213, bacillus mojavensis b-1469 ... | 2017 | 27687995 |
| overproduction and characterization of a lytic polysaccharide monooxygenase in bacillus subtilis using an assay based on ascorbate consumption. | lytic polysaccharide monooxygenases (lpmos) are copper ion-containing enzymes that degrade crystalline polysaccharides, such as cellulose or chitin, through an oxidative mechanism. to the best of our knowledge, there are no assay methods for the direct characterization of lpmos that degrade substrates without coupled enzymes. as such, in this study, a coupled enzyme-free assay method for lpmos was developed, which is based on measuring the consumption of ascorbic acid used as an external electro ... | 2016 | 27702475 |
| production of lipopeptide biosurfactants by bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery. | lipopeptides are known as promising microbial surfactants and have been successfully used in enhancing oil recovery in extreme environmental conditions. a biosurfactant-producing strain, bacillus atrophaeus 5-2a, was recently isolated from an oil-contaminated soil in the ansai oilfield, northwest china. in this study, we evaluated the crude oil removal efficiency of lipopeptide biosurfactants produced by b. atrophaeus 5-2a and their feasibility for use in microbial enhanced oil recovery. | 2016 | 27716284 |
| evaluating composite sampling methods of bacillus spores at low concentrations. | restoring all facility operations after the 2001 amerithrax attacks took years to complete, highlighting the need to reduce remediation time. some of the most time intensive tasks were environmental sampling and sample analyses. composite sampling allows disparate samples to be combined, with only a single analysis needed, making it a promising method to reduce response times. we developed a statistical experimental design to test three different composite sampling methods: 1) single medium sing ... | 2016 | 27736999 |
| effect of static magnetic field on endospore germination. | this work investigated the effect of static magnetic field (smf) on bacillus atrophaeus endospore germination. germination was triggered by l-alanine in 1.3-t smf and characterized by ion release, ca(2+) -dipicolinic acid release, and water influx. these events were monitored by electrical conductivity, tb-dpa fluorescence, and optical density, respectively. culturability of endospore germinated in smf exposure was evaluated by cfu enumeration. results indicated that 1.3-t smf failed to signific ... | 2017 | 27862061 |
| highly sensitive sequence specific qpcr detection of mycobacterium tuberculosis complex in respiratory specimens. | nucleic acid amplification tests for mycobacterium tuberculosis (mtb) detection from sputum are highly sensitive and specific with smear microscopy positive specimens, but their sensitivity with smear-negative/culture-positive specimens is much lower; therefore, these tests cannot rule out a tuberculosis diagnosis. co-extraction of pcr inhibitors may be a cause of decreased test sensitivity. here the design and early validation of a mtb screening assay with sample preparation and qpcr methods de ... | 2016 | 27865380 |
| heterologous expression of γ-glutamyl transpeptidase from bacillus atrophaeus gs-16 and its application in the synthesis of γ-d-glutamyl-l-tryptophan, a known immunomodulatory peptide. | gamma-glutamyl transpeptidase from a mesophilic bacterium bacillus atrophaeus gs-16 (baggt) was expressed heterologously in e. coli using pet-51b vector. maximum production of baggt was obtained at 16°c after 16h of iptg induction and the protein, in its native conformation, was active as a heterooctamer which was composed of four heterodimeric units combined together. one heterodimeric unit constituted two subunits with molecular masses of 45kda and 21kda, respectively. the recombinant enzyme w ... | 2017 | 28193333 |
| comparative analysis of the sensitivity of metagenomic sequencing and pcr to detect a biowarfare simulant (bacillus atrophaeus) in soil samples. | to evaluate the sensitivity of high-throughput dna sequencing for monitoring biowarfare agents in the environment, we analysed soil samples inoculated with different amounts of bacillus atrophaeus, a surrogate organism for bacillus anthracis. the soil samples considered were a poorly carbonated soil of the silty sand class, and a highly carbonated soil of the silt class. control soil samples and soil samples inoculated with 10, 103, or 105 cfu were processed for dna extraction. about 1% of the d ... | 2017 | 28472119 |
| effect of bacteria type and sucrose concentration on levan yield and its molecular weight. | levan has been traditionally produced from microorganism. however, there is a continuous effort in looking for new strains that improve levan production yield and uses alternative sugar sources for growth. despite having a wide range of data about levan yield, there are not papers which allow controlling molecular weight, and that plays an essential role for further applications. | 2017 | 28535808 |
| squamocin, an annonaceous acetogenin, enhances naphthalene degradation mediated by bacillus atrophaeus cn4. | squamocin belongs to a group of compounds called annonaceous acetogenins. they are secondary products of annonaceae metabolism and can be isolated from annona cherimolia seeds. this paper deals with the stimulation of biofilm formation of bacillus atrophaeus cn4 by employing low squamocin concentrations to increase naphthalene degradation. bacillus atrophaeus cn4, isolated from contaminated soil, has the ability to degrade naphthalene as the only source of carbon and energy. in the absence of ad ... | 2017 | 28554707 |
| is the stella™ 5l system an effective cold sterilization technique for needle-based confocal miniprobes? | needle-based confocal laser endomicroscopy (ncle) under endoscopic ultrasound guidance is a newly developed imaging technique for pancreatic lesions and lymph nodes, enabling a subcellular level of resolution. the confocal miniprobe is an invasive instrument designed to be reused up to 10 times. therefore, a method that ensures the complete elimination of microbial contaminants on the device is necessary. we studied the bactericidal efficacy of the stella™ system, which purports to achieve this ... | 2017 | 28621298 |