Publications

mechanism of resistance to vancomycin in enterococcus faecium d366 and enterococcus faecalis a256.	the role of the glycopeptide-inducible proteins of enterococcus faecium d366 (39.5 kilodaltons) and enterococcus faecalis a256 (39 kilodaltons) in the mechanism of resistance to vancomycin and teicoplanin was examined. crude cell walls from noninduced cells or from induced cells treated with sodium dodecyl sulfate to remove the inducible proteins were shown to bind vancomycin, in contrast to cell walls containing the cytoplasmic membrane-associated induced proteins, which did not bind vancomycin ...	1990	2139314
antibacterial activity of the leaves of phyllanthus discoideus.	the lyophilized aqueous extract (lwe) from the leaves of phyllanthus discoideus was found to show an antibacterial activity. the alkaloid fraction obtained from lwe inhibited the growth of escherichia coli and enterococcus faecium (mic = 1.6 mg/ml), pseudomonas aeruginosa (mic = 0.78 mg/ml), staphylococcus aureus and mycobacterium smegmatis (mic = 0.2 mg/ml). among the alkaloids identified, viroallosecurinine and securinine showed a high activity. viroallosecurinine exhibited a mic of 0.48 micro ...	1990	2156112
emergence of 4',4"-aminoglycoside nucleotidyltransferase in enterococci.	enterococcus faecium bm4102 was resistant to macrolide-lincosamide-streptogramin b-type (mls) antibiotics; tetracycline-minocycline; and high levels of kanamycin, neomycin, tobramycin, and dibekacin but not gentamicin. this aminoglycoside resistance phenotype is new in enterococci. the genes conferring resistance to aminoglycosides and mls antibiotics in this strain were carried on a plasmid, pip810, that was self-transferable to to other enterococcus strains. resistance to tobramycin and struct ...	1990	2171424
association of bacteria with the fungal fermentation of soybean tempe.	bacteria grew to viable populations of 10(8)-10(9) cfu/g during the fermentation of soybeans into tempe with the fungus, rhizopus oligosporus. bacillus pumilus and b. brevis were the predominant bacterial species, reaching populations of approximately 10(8) cfu/g during the 48 h fermentation. species of streptococcus faecium, lactobacillus casei, klebsiella pneumoniae and enterobacter cloacae also contributed to the fermentation and achieved populations of 10(6)-10(7) cfu/g.	1990	2179196
a double-blind, controlled trial of bioflorin (streptococcus faecium sf68) in adults with acute diarrhea due to vibrio cholerae and enterotoxigenic escherichia coli.	the therapeutic efficacy of bioflorin (streptococcus faecium sf68; gipharmex, milan, italy) in acute watery diarrhea was evaluated in 183 bangladeshi adults. vibrio cholerae organisms were isolated from stool cultures in 114 patients, and enterotoxigenic escherichia coli organisms were isolated in 41. in addition to iv rehydration, patients were randomly assigned to receive either capsules of bioflorin containing 1 x 10(9) of live sf68 or capsules of placebo containing killed sf68 once every 8 h ...	1990	2203662
in vitro activity of pd127,391, a new quinolone against bacterial isolates from cancer patients.	the in vitro activity of pd127,391, a new 4-quinolone, was compared to that of ciprofloxacin against common clinical bacterial isolates from patients with cancer. pd127,391 was found to have a broad antimicrobial spectrum with excellent activity against gram-positive isolates (including multidrug-resistant organism such as corynebacterium jeikeium, enterococcus faecalis, enterococcus faecium, methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp.). it was also ext ...	1990	2209169
induction of vancomycin resistance in enterococcus faecium by inhibition of transglycosylation.	vancomycin resistance has recently been recognized among clinical isolates of enterococci. resistance is inducible, and associated with production of a novel 39 kda membrane protein. the mechanism by which exposure to vancomycin, which does not penetrate the cell membrane, induces resistance is unknown. in the vancomycin resistant strain enterococcus faecium 228, resistance was also inducible by moenomycin, suggesting that inhibition of the transglycosylation step in peptidoglycan synthesis may ...	1990	2227352
influence of dietary supplementation with streptococcus faecium m-74 on broiler body weight, feed conversion, carcass characteristics, and intestinal microbial colonization.	two experiments were conducted to determine the influence of streptococcus faecium m-74 supplementation of broiler diets. mixed-sex or male only chicks were used in the experiments. both experiments consisted of six dietary treatments and four replicates per treatment in a randomized block design. the s. faecium was fed alone for 21, 36, or 44 days or for 44 days with s. faecium supplemented in the water for the first 14 days. in another treatment, s. faecium was fed for 44 days, but the feed wa ...	1990	2235841
the vana glycopeptide resistance protein is related to d-alanyl-d-alanine ligase cell wall biosynthesis enzymes.	inducible resistance to the glycopeptide antibiotics vancomycin and teicoplanin is mediated by plasmid pip816 in enterococcus faecium strain bm4147. vancomycin induced the synthesis of a ca. 40 kda membrane-associated protein designated vana. the resistance protein was partially purified and its n-terminal sequence was determined. a 1761 bp dna restriction fragment of pip816 was cloned into escherichia coli and sequenced. when expressed in e. coli, this fragment encoded a ca. 40 kda protein that ...	1990	2266943
treatment of nosocomial infections in children undergoing antimicrobial chemotherapy.	patients under immunosuppressive therapy with malignant diseases, malformations, premature infants or children after major surgical interventions and trauma are particularly susceptible to infections. in these patients nosocomial infections with multiply resistant organisms may occur despite broad spectrum antibiotic prophylaxis or antimicrobial chemotherapy of existing infections. in an open clinical study 31 infants and children with an overall 45 episodes of life-threatening hospital-acquired ...	1990	2276826
phenotypic and genotypic heterogeneity of glycopeptide resistance determinants in gram-positive bacteria.	gram-positive glycopeptide-resistant bacteria isolated in various hospitals in europe and in the united states between 1986 and 1988 were collected. three resistance phenotypes could be distinguished. thirty-one enterococci were highly resistant to vancomycin and teicoplanin. resistance was transferable to other enterococci by conjugation for 16 of the 22 isolates that were tested. homology was detected by hybridization between a probe specific for the vana gene, which encodes an inducible high- ...	1990	2291654
correlation of penicillin-induced lysis of enterococcus faecium with saturation of essential penicillin-binding proteins and release of lipoteichoic acid.	clinical isolates of enterococcus faecium that had a range of susceptibilities to penicillin were found to differ significantly in their responses to the antibiotic. in the penicillin-susceptible group (mic, less than or equal to 4 micrograms/ml), the cessation of growth (bacteriostasis) at 10 x the mic of penicillin appeared to correlate with the inhibition of penicillin-binding protein (pbp) 5*, whereas the onset of lysis (bactericidal effect) at higher antibiotic concentrations (100 x the mic ...	1990	2291656
microbiologic characteristics of lactobacillus products used for colonization of the vagina.	lactobacilli have long been considered to be the protective flora in the vagina. women with vaginal infections have used various non-prescription products in an attempt to restore their normal vaginal flora. products that contain lactobacilli include dairy products (yogurt, acidophilus milk) and commercially available lactobacillus powders and tablets. recently, lactobacillus species that produce hydrogen peroxide (h2o2) have been associated with normal vaginal flora. in this study, we compared ...	1990	2300352
enterococcal resistance to vancomycin and related cyclic glycopeptide antibiotics.	enterococci belonging to various species resistant to vancomycin and related cyclic glycopeptide antibiotics have been isolated from hospitalized patients in france, the uk and the usa. all such strains examined display inducible synthesis of a membrane protein associated with resistance. the mechanism by which the membrane protein acts has not been definitively established, but it may block the access of the antibiotic to its peptidoglycan target. that the protein could be a bypass enzyme has n ...	1990	2318215
vancomycin resistance is encoded on a pheromone response plasmid in enterococcus faecium 228.	in enterococcus faecium 228, vancomycin resistance is encoded on a 55-kilobase conjugative plasmid, phkk100. this plasmid was transferred with high frequency into susceptible strains of enterococcus faecalis and conferred responses to pheromones produced by e. faecalis and streptococcus sanguis. phkk100 is the first plasmid described that mediates both vancomycin resistance and pheromone response.	1990	2327781
cloning and heterospecific expression of the resistance determinant vana encoding high-level resistance to glycopeptides in enterococcus faecium bm4147.	fragments of plasmid pip816, which confers high-level glycopeptide resistance in enterococcus faecium bm4147, were cloned into a conjugative gram-negative-gram-positive shuttle vector. the resulting hybrids were transferred by conjugation from escherichia coli to enterococcus faecalis and bacillus thuringiensis. a 4-kilobase ecori fragment from pip816 was found to confer vancomycin resistance in these hosts but not in e. coli or bacillus subtilis.	1990	2360831
properties of cell wall-associated dd-carboxypeptidase of enterococcus hirae (streptococcus faecium) atcc 9790 extracted with alkali.	dd-carboxypeptidase (dd-cpase) activity of enterococcus hirae (streptococcus faecium) atcc 9790 was extracted from intact bacteria and from the insoluble residue (crude cell wall fraction) of mechanically disrupted bacteria by a brief treatment at ph 10.0 (10 mm glycine-naoh) at 0 degrees c or by extraction with any of several detergents. extractions with high salt concentrations failed to remove dd-cpase activity from the crude wall fraction. in contrast to n-acetylmuramoylhydrolase (both muram ...	1990	2361945
spontaneous peritonitis caused by enterococcus faecium.	three cases of spontaneous peritonitis caused by enterococcus faecium are presented. the underlying condition was alcoholic cirrhosis in each case. this enterococcal species has never before been reported as a cause of spontaneous bacterial peritonitis. two patients responded to therapy. the development of enterococcal peritonitis and the cases documented in the literature are briefly reviewed. taxonomic problems with pathogenic, clinical, and therapeutic implications are discussed.	1990	2380371
comparative in vitro activity of win 57273, a new fluoroquinolone antimicrobial agent.	the in vitro activity of win 57273, a new fluoroquinolone antimicrobial agent, was evaluated against approximately 600 bacterial isolates. the new drug was 4- to 128-fold more active than ciprofloxacin against a broad range of gram-positive organisms, with the new drug inhibiting 90% of strains of each species except enterococcus faecium at concentrations of less than or equal to 0.25 microgram/ml. win 57273 was four- to eightfold less active than ciprofloxacin against many members of the family ...	1990	2393275
technologically important characteristics of enterococcus isolates from milk and dairy products.	enterococcus faecium (54 strains), e. faecalis (40 strains), and e. durans (14) were isolated from various dairy products (raw milk, cream, butter and fermented milk products) during a previous study (wessels et al., 1988). in this article various characteristics of these isolates, which may have a bearing on their significance in dairy products, have been studied. a large percentage of the identified strains of all three species were able to grow at 7 degrees c. seventy-six percent of the e. fa ...	1990	2397162
analysis of nutritional shift-up of streptococcus faecium.	three-dimensional reconstruction methods were applied to electron micrographs of streptococcus faecium to study the initiation of cell wall growth sites during a nutritional shift-up experiment. upon lowering the mass doubling time from 76 to 33 min by the addition of excess glutamate, the formation of new cell wall growth sites was studied in relation to other growth parameters (autolytic capacity, cell number, mass, rna, dna and peptidoglycan). the findings from these studies, to be described ...	1985	2408555
syntheses and antifolate activity of 5-methyl-5-deaza analogues of aminopterin, methotrexate, folic acid, and n10-methylfolic acid.	evidence indicating that modifications at the 5- and 10-positions of classical folic acid antimetabolites lead to compounds with favorable differential membrane transport in tumor vs. normal proliferative tissue prompted an investigation of 5-alkyl-5-deaza analogues. 2-amino-4-methyl-3,5-pyridinedicarbonitrile, prepared by hydrogenolysis of its known 6-chloro precursor, was treated with guanidine to give 2,4-diamino-5-methylpyrido[2,3-d]pyrimidine-6-carbonitrile which was converted via the corre ...	1986	2423690
autoradiographic studies of the synthesis of rna and protein as a function of cell volume in streptococcus faecium.	mid-exponential-phase cultures were either labeled continuously with tritiated leucine and uracil or pulse-labeled with tritiated leucine. the amount of leucine and uracil incorporated into protein or rna per cell was determined by grain counts of autoradiographs of cells seen in electron micrographs; the volume of each cell was determined by three-dimensional reconstruction. the average number of autoradiographic grains around cells continuously labeled with uracil and leucine increased linearl ...	1986	2427501
synthesis and biological evaluation of poly-gamma-glutamyl metabolites of 10-deazaaminopterin and 10-ethyl-10-deazaaminopterin.	the chemical synthesis of a series of poly-gamma-glutamyl metabolites of the experimental anticancer drugs 10-deazaaminopterin (10-daam) and 10-ethyl-10-deazaaminopterin (10-edaam) has been carried out by the solid-phase procedure. the synthetic products were identical with the poly-gamma-glutamyl metabolites of radiolabeled 10-daam and 10-edaam produced by normal mouse tissues with regard to elution volume from [(diethylamino)ethyl]cellulose columns and susceptibility to hydrolysis by human pla ...	1988	2447278
[role of enterococcus faecium sf 68 in experimental infection in mice].	the normal intestinal flora is an important defence against various infectious agents. it also allows important metabolic stages in the host. the authors studied the ability of enterococcus faecium sf 68 to protect the mice (swiss strain) from infection by salmonella typhi and its role when used with penicillin in infection by staphylococcus aureus. our results showed that sf 68, determined a good protection against infection. moreover the association e. faecium sf 68-antibiotic lowers the morta ...	1989	2488915
atypical transient state kinetics of recombinant human dihydrofolate reductase produced by hysteretic behavior. comparison with dihydrofolate reductases from other sources.	the transient state kinetics of catalysis for dihydrofolate reductase (dhfr) from several enzyme sources including highly purified recombinant human enzyme (rhdhfr) have been examined. like dhfr from escherichia coli, the enzyme from lactobacillus casei, and isoenzyme 2 from streptococcus faecium exhibit a slow increase in activity upon addition of substrates to enzyme. no slow hysteresis of this type was detected with recombinant human dhfr (rhdhfr) or dhfr from chicken or bovine liver or l1210 ...	1989	2492521
in vitro activities of quinolones against enterococci resistant to penicillin-aminoglycoside synergy.	the mics and mbcs of ci-934, ciprofloxacin, difloxacin (a-56619), a-56620, norfloxacin, enoxacin, amifloxacin, and coumermycin were determined for 43 clinical isolates of enterococcus faecalis known to be resistant to penicillin-aminoglycoside synergy. results were compared with those obtained for 37 synergy-susceptible e. faecalis and 22 enterococcus faecium strains. although no substantial differences in quinolone activities were observed between synergy-resistant and -susceptible e. faecalis ...	1989	2496659
in vitro activity of ampicillin/sulbactam against enterococci determined by the time-kill method.	time-kill studies demonstrated that at clinically achievable serum concentrations, ampicillin/sulbactam was equivalent in activity to ampicillin alone against non-beta-lactamase producing isolates of enterococcus faecalis and enterococcus faecium. sulbactam possessed no antibacterial activity against these organisms. it is not yet known if the activity of ampicillin will be increased with the addition of sulbactam when tested against beta-lactamase-producing enterococci.	1989	2496949
folate analogues. 31. synthesis of the reduced derivatives of 11-deazahomofolic acid, 10-methyl-11-deazahomofolic acid, and their evaluation as inhibitors of glycinamide ribonucleotide formyltransferase.	the boon-leigh procedure, involving condensation of a 6-chloro-5-nitropyrimidine (22) with an alpha-amino ketone (20 or 21) followed by reduction of the nitro group, cyclization, and l-glutamylation, led to the formation of 11-deazahomofolate (29) and its 10-methyl derivative (30). the corresponding (6r,s)-5,6,7,8-tetrahydro (4, 5) and 7,8-dihydro (31, 32) derivatives were prepared by catalytic hydrogenation. (6s)-11-deazatetrahydrohomofolate was prepared from 29 by enzymatic reduction. compound ...	1989	2498518
inducible, transferable resistance to vancomycin in enterococcus faecium, d399.	enterococcus faecium d399 was isolated from the blood and peritoneal abscess of a patient with intraabdominal sepsis. the patient had not been treated with vancomycin, but the strain was found to be resistant with a mic of 1000 mg/l. resistance was inducible and transferable (probably by conjugation) to jh2-2, and correlated with induction of synthesis of a 39 kda protein. this mechanism appears to be identical to that previously described for e. faecalis a256, suggesting that dissemination of t ...	1989	2501270
[the effect of roxithromycin on the aerobic fecal flora in children].	to evaluate the effect of roxithromycin on aerobic flora in 14 children treated orally (5 mg/kg/day during 5 to 7 days) quantitative stool cultures were performed using selective media. enterobacteriaceae (eb), streptococci d (str d), staphylococcus aureus, candida were counted at days 0, 2 and 5 days, and 2 days after treatment. the mic of each strain was determined for roxithromycin, erythromycin and spiramycin. ten infants in a nursery had already received antibiotics. a moderate albeit signi ...	1989	2506510
disinfection with gaseous formaldehyde. third part: bactericidal and sporicidal effectiveness of gaseous formaldehyde and level of residues in dependence on concentration, temperature and relative humidity.	the highest rate of inactivation of staphylococcus aureus (atcc 6538), streptococcus faecium (atcc 6057) and spores of bacillus subtilis var. niger (dsm 675) was observed at a relative humidity (rh) of 90-95%. at a rh of 60% d 0.75 mg hcho l-1 air at 45 degrees c-values of 35 min and over 65 min were determined for s. aureus and for b. subtilis spores which decreased to d = 2.9 min and d = 20 min respectively at a rh of 90%. at 45 degrees c, an optimal formaldehyde (hcho) concentration for the i ...	1989	2508656
in vitro antibacterial activity and beta-lactamase stability of the new carbapenem sm-7338.	the in vitro activity of the new carbapenem sm-7338 was tested in comparison with imipenem, ceftazidime, cefotaxime, flomoxef, cefuzonam and cefmetazole against 2850 clinical bacterial isolates. sm-7338 showed good activity against a broad spectrum of gram-positive and gram-negative bacteria. sm-7338 was very active against gram-negative bacteria, inhibiting all enterobacteriaceae, except 25% of serratia marcescens isolates, at a concentration of 0.78 mg/l. sm-7338 inhibited the majority of pseu ...	1989	2512140
the microbial ecology of soybean soaking for tempe production.	soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ...	1989	2518226
transferable vancomycin and teicoplanin resistance in enterococcus faecium.	enterococcus faecium bm4165 and bm4178, isolated from immunocompromised patients, one treated with vancomycin, were inducibly resistant to high levels of the glycopeptide antibiotics vancomycin and teicoplanin but susceptible to the new lipopeptide daptomycin (ly146032). strain bm4165 was also resistant to macrolidelincosamide-streptogramin b-type (mls) antibiotics. the genes conferring resistance to glycopeptides and to mls antibiotics in strain bm4165 were carried on plasmids pip819 and pip821 ...	1989	2523687
activity of various glycopeptides against an inducibly vancomycin-resistant strain of enterococcus faecium (d366).		1989	2524533
therapy of serious gram-positive infections with teicoplanin.	teicoplanin was used as monotherapy in 17 seriously ill patients (mean age 51 years, range 18-71) with infection due to gram-positive organisms. the dose used was 200 mg teicoplanin intravenously once daily after an initial intravenous dose of 400 mg. most of the patients (15 out of 17) were septicaemic (confirmed by blood culture), the other two had deep abscesses. the pathogens isolated were staphylococcus epidermidis [15], streptococcus faecium [1] and jk coryneform [1]. the 15 patients with ...	1989	2526010
characterization of vancomycin resistance in enterococcus faecium and enterococcus faecalis.	vancomycin resistance in enterococcus faecium 180, a clinical isolate from england, was studied. resistance to vancomycin was transferable by conjugation to other enterococci. expression of resistance was inducible and coincided with the appearance of a new membrane protein.	1989	2528940
variation in buoyant density of whole cells and isolated cell walls of streptococcus faecium (atcc 9790).	the buoyant density of whole cells of streptococcus faecium varies with growth rate and during the cell cycle. two possible explanations for this were explored: (i) the density of cell walls may vary, and (ii) the proportions of wall and cytoplasm may vary. we tested the first possibility by isolating walls from chilled, unfixed populations of s. faecium cells and fractionating them on percoll density gradients. mean cell wall density averaged 4% less than whole-cell density and did not vary sig ...	1989	2549008
transposon-916-like elements in clinical isolates of enterococcus faecium.	tetracycline (tc) resistance was found in nine out of ten clinical isolates of enterococcus faecium. conjugative transposons, designated tn5031, tn5032 and tn5033, were present in the chromosome of three isolates. the transposons were similar both structurally and functionally to tn916 containing the tetm determinant. a large non-conjugative plasmid found in a fourth isolate contained an element homologous to tn916. the four isolates containing the element showing homology to tn916 exhibited a s ...	1989	2559146
dna probe for identification of streptococcus pneumoniae.	a total of 287 clinical isolates of streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. the test was positive for all but one isolate, strain doc-1. this autolysis required the activity of an enzyme which is unique and characteristic of s. pneumoniae: a choline-dependent n-acetylmuramoyl-l-alanine amidase, the gene product of the lyta gene. we used lyta as a dna probe to test the distribution of the autolysin gene amon ...	1989	2563386
at2433-a1, at2433-a2, at2433-b1, and at2433-b2 novel antitumor antibiotic compounds produced by actinomadura melliaura. taxonomy, fermentation, isolation and biological properties.	compounds at2433-a1 (a1), at2433-a2 (a2), at2433-b1 (b1), and at2433-b2 (b2) were isolated from the cultured broth of actinomadura melliaura sp. nov. (scc 1655). structurally these materials are closely related to rebeccamycin (1), an indolocarbazole antitumor antibiotic. a1, a2, b1, and b2 were active against staphylococcus aureus a9537, streptococcus faecalis a20688, streptococcus faecium (atcc 9790), micrococcus lutea (atcc 9341), bacillus subtilis (atcc 6633). a1 and b1 were active against p ...	1989	2584136
[the effect of enterococci on normalizing the intestinal microflora in experimental dysbacteriosis].	the normal microflora of the intestine produces essential influence on the vital activity of the host. the exposure of the body to the action of different unfavorable factors (roentgen radiation, the administration of antibiotics, salmonella infection, etc) results in changes in the normal microflora of the gastrointestinal tract. this work was aimed at the study of the influence of streptococcus faecium ydc-48 on the intestinal microflora of mice in experimental (chemotherapeutic, postirradiati ...	1989	2588877
the bactericidal effect of isoascorbic acid combined with mild heat.	the thermal inactivation of salmonella thompson, escherichia coli, staphylococcus aureus, clostridium perfringens, candida zeylanoides, enterococcus faecium and e. faecalis was accelerated by the addition of sodium isoascorbate (1 mmol/l) to phosphate-buffer heating medium but not to complex food mixtures. the lethal effect of isoascorbate was nullified by heating under anaerobic conditions or by the addition of catalase. the scavengers of hydroxyl radicals, mannitol and formate were not protect ...	1989	2613595
[a case report of meningitis and sepsis due to enterococcus faecium complicated with strongyloidiasis].	a 80 year-old male was transferred to our department on 18th aug. 1988, for high fever and clouding of the consciousness. he had been treated with steroid hormone (betamethasone 3.0 mg/day for 15 days) for his uveitis. enterococcus faecium was isolated from both blood and spinal fluid, and then strongyloides sterocoralis was revealed both in the sputum and stool. anti-human t-cell leukemia virus 1 (htlv-1) antibody was also positive serologically. at first, beta-lactam antibiotics were used for ...	1989	2621387
effect of cell cycle stages on the central density of enterococcus faecium atcc 9790.	cultures of enterococcus faecium growing at various rates were examined for timing of cell division cycle events by using the method of residual divisions and a morphological analysis. both methods gave essentially the same timing for the onset of d1 (completion of chromosome replication) and of d2 (completion of septation). frequencies of cells exhibiting a phase-reversed center in bovine serum albumin at various growth rates were determined. the data fit a model in which rapidly growing cells ...	1989	2649481
bacterial hydrophobicity, an overall parameter for the measurement of adhesion potential to soil particles.	the adhesion of salmonella typhimurium to the mineral particles quartz, albite, feldspar, and magnetite was shown to correlate with the hydrophobicity of the cell surface as measured by hydrophobic interaction chromatography. the same effects were also seen for seven other selected test strains, including streptococcus faecalis, streptococcus faecium, escherichia coli, citrobacter freundii, shigella sonnei, and shigella boydii. when the test strain of salmonella typhimurium, was repeatedly culti ...	1989	2650618
paraghurt for patients with irritable bowel syndrome. a controlled clinical investigation from general practice.	a comparison of paraghurt tablets (freeze-dried culture of streptococcus faecium) and placebo was carried out in patients with irritable bowel syndrome in a double-blind trial in danish general practices. fifty-four patients (42 females and 12 males) with an average duration of the disease of seven years were treated for four weeks. the clinical symptoms were registered prior to the treatment and after two and four weeks. the clinical effect was assessed according to changes in number of abdomin ...	1989	2657953
fce 22101 and fce 22891: in-vitro antibacterial activity at concentrations simulating human plasma levels following intravenous, intramuscular and oral administration.	the plasma concentrations of fce 22101 observed following slow intravenous infusions (1 and 4 h), intramuscular injection and after oral administration of the prodrug fce 22891 were simulated in a glass chamber containing bacterial cultures. the bacteria used were staphylococcus aureus atcc 13709, staph. aureus 2101 (methicillin-resistant), streptococcus faecium atcc 8043, escherichia coli atcc 12407, enterobacter cloacae 1321e and ent. cloacae p99 (cefazolin-resistant). addition of fce 22101 wa ...	1989	2659570
[susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates].	susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ...	1989	2664255
disinfection with gaseous formaldehyde. first part: bactericidal and sporicidal effectiveness of formaldehyde with and without formation of a condensing layer.	suspensions of staphylococcus aureus atcc 6538, streptococcus faecium atcc 6057, and of spores of bacillus subtilis var. niger dsm 675 dried on polished stainless steel carriers were exposed in a model chamber to 3.2 mg hcho l-1 air at temperatures of 20, 25, 30, 35 and 40 degrees c and the kinetics of their inactivation were determined by successive colony counting during the exposures. the hcho treatment was carried out once with the formation of a condensing layer on the carriers and once wit ...	1989	2667552
[ileovesical fistula complicating crohn's disease: a case report].	a 43-year-old woman was admitted on october 6, 1987 with the chief complaint of fecaluria, pneumaturia and miction pain. she had been diagnosed as crohn's disease in march, 1987. urinalysis revealed numerous leucocytes, and streptococcus faecium was identified by urine culture. contrast film of small intestines showed ileovesical fistula arising from terminal ileum. cystoscopy revealed a papillary tumor-like appearance at the dome of the bladder. an operation was performed on november 9 under th ...	1989	2678984
the enterococci: evidence of species-specific clinical and microbiologic heterogeneity.	investigations of the clinical role of enterococci have been limited largely to enterococcal bacteremia and endocarditis and have not distinguished between the various species of enterococci. to characterize the full spectrum of enterococcal disease and to determine whether clinically important differences exist among infections and/or instances of colonization (infections/colonizations) by the various enterococcal species, cases of enterococcal infection/colonization diagnosed at the dallas vet ...	1989	2705463
penicillin-aminoglycoside synergy and post-antibiotic effect for enterococci.	the post-antibiotic effect (pae) on seven strains of enterococci was studied using a novel atp method and conventional viable counting. there was a good correlation between the results of the two techniques. in strains exhibiting low-level resistance to penicillin and streptomycin, paes of 1 to 2.4 h (mean 1.8 h) resulted from exposure to penicillin; shorter paes were induced by streptomycin (0.2-0.3 h; mean 0.2 h). addition of streptomycin to pencillin increased the duration of penicillin-induc ...	1989	2708179
identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria.	a total of 84 of 150 vancomycin-resistant (defined as no inhibition of bacterial growth around a 30-micrograms vancomycin disk placed on 5% sheep blood-trypticase soy agar [bbl microbiology systems, cockeysville, md.]) bacteria were definitively identified by determining the phenotypic criteria. the identity of representatives was also confirmed by dna-dna hybridizations. the following strains were identified: 1 enterococcus faecium, 18 leuconostoc mesenteroides, 15 leuconostoc citreum, 9 leucon ...	1989	2723037
inducible resistance to vancomycin in enterococcus faecium d366.	strain d366, a clinical isolate of enterococcus faecium, is resistant (minimum inhibitory concentration [mic] 32 mg/l) to vancomycin. when exponential-phase cultures were exposed to half the mic of vancomycin, a lag of 3-4 h occurred before growth resumed. cells preexposed to 1/2 mics of vancomycin did not show any lag. pregrowth of d366 with vancomycin caused resistance to all glycopeptides tested. pregrowth in vancomycin resulted in synthesis of a 3.95-kda cytoplasmic-membrane-associated prote ...	1989	2723455
aerococcus-like organism--an unnoticed urinary tract pathogen.	aerococci are only occasionally isolated from urinary tract specimens. a 4-month' survey revealed 29 patients suspected of urinary tract infection (uti), from whom an aerococcus-like organism (alo) was isolated in significant numbers from urinary tract specimens and singly in 11 cases. approximately 0.8% of all urinary tract specimens examined during the period yielded growth of alo. the median age of patients was 75 years. half of the patients were found to have conditions predisposing to uti. ...	1989	2736106
studies on faecal streptococci in the river tigris.	six hundred water samples collected from the river tigris at mosul city were investigated for faecal streptococci. human faecal streptococci were predominant, and animal faecal streptococci were also detected. eight species and varieties were identified, viz streptococcus faecalis, atypical streptococcus faecalis, streptococcus bovis, streptococcus equinus, streptococcus faecalis var. liquefaciens, streptococcus faecalis var. zymogenes, streptococcus durans and streptococcus faecium. the inciden ...	1989	2739584
the second peptidoglycan hydrolase of streptococcus faecium atcc 9790 covalently binds penicillin.	a second peptidoglycan hydrolase (muramidase-2) of streptococcus faecium atcc 9790 (enterococcus hirae) has been purified to apparent homogeneity. the enzyme has been shown to be a beta-1,4-n-acetylmuramoylhydrolase (muramidase; ec 3.2.1.17) and to differ in substrate specificity from a previously isolated muramidase. purified enzyme appears as two protein staining bands with molecular masses of 125 and 75 kilodaltons (kda) on polyacrylamide gels after sodium dodecyl sulfate electrophoresis. elu ...	1989	2753858
[lactic bacteria in the digestive tract of poultry].	lactic bacteria predominate in the microflora of the digestive tract of chicken and turkey. they are represented mainly by lactobacillus acidophilus, l. salivarius, l. fermentum and l. buchneri. streptococcus faecium is always isolated. l. ruminis, l. vitulinus, l. delbrueckii, l. coryniformis and l. viridescens were found in this ecological niche for the first time. s. faecium and s. faecalis prevail in the digestive tract of geese and ducks, while lactobacilli are detected in a lesser amount a ...	1989	2761452
emergence of isolates resistant to ampicillin.	clinical isolates of streptococcus faecium demonstrating ampicillin resistance were recovered from eight pediatric patients. sites of isolation included blood, surgical wound, bile drainage, urine, burns, and peritoneal fluid. seven patients had prolonged hospitalization, and all had been treated with broad-spectrum antibiotics prior to isolation of the resistant enterococcus. one isolate was from an ill, bacteremic patient; the others were in mixed culture and were not considered causes of dise ...	1989	2773881
enterococci highly resistant to penicillin and ampicillin: an emerging clinical problem?	sixteen clinical isolates of ampicillin-resistant enterococci (are) were recovered from the microbiology laboratory of a 450-bed rehabilitation medical center from january 1981 to september 1987. these isolates were detected when a disk diffusion test using 10 micrograms of ampicillin on a blood agar plate revealed no zones of inhibition. tube macrodilution tests yielded an mic of greater than or equal to 16 micrograms of ampicillin per ml. none of the isolates were penicillinase producers by th ...	1989	2778072
a protein of unusual composition from enterococcus faecium.		1989	2780297
tricyclic 2,4-diaminopyrimidines with broad antifolate activity and the ability to inhibit pneumocystis carinii growth in cultured human lung fibroblasts in the presence of leucovorin.	a selected number of 1,3-diaminobenzo[f]quinazolines and 1,3-diamino-5,6-dihydrobenzo[f]quinazolines, which may be viewed as tricyclic analogues of the lipid-soluble antifolates pyrimethamine (pm), metoprine (ddmp), and etoprine (ddep), were tested as inhibitors of purified dihydrofolate reductase (dhfr) from wi-l2 lymphoblasts, and as inhibitors of the growth of streptococcus faecium atcc 8043 and l1210 murine leukemia cells in culture. in addition, these tricyclic compounds were tested for ant ...	1989	2788420
active-site and membrane topology of the dd-peptidase/penicillin-binding protein no. 6 of enterococcus hirae (streptococcus faecium) a.t.c.c. 9790.	the membrane-bound 43,000-mr penicillin-binding protein no. 6 (pbp6) of enterococcus hirae consists of a 30,000-mr dd-peptidase/penicillin-binding domain and a approximately 130-residue c-terminal appendage. removal of this appendage by trypsin proteolysis has no marked effect on the catalytic activity and penicillin-binding capacity of the pbp. anchorage of the pbp in the membrane appears to be mediated by a short 15-20-residue stretch at the c-terminal end of the appendage. the sequence of the ...	1989	2803261
comparative in-vitro activity of meropenem on clinical isolates from the united kingdom.	mics of meropenem were determined for a wide range of common bacteria of clinical importance. for enterobacteriaceae, aeromonas spp., haemophilus influenzae, branhamella catarrhalis, neisseria gonorrhoeae, gardnerella vaginalis, campylobacter coli/jejuni, beta-haemolytic streptococci and anaerobes other than clostridium difficile, mics were almost always within the range 0.002-0.5 mg/l. the activity of meropenem for these organisms was always greater than that of imipenem and piperacillin, and w ...	1989	2808214
activity of glycopeptides against vancomycin-resistant gram-positive bacteria.	gram-positive bacteria resistant to vancomycin are rare; but they include members of the genera leuconostoc, lactobacillus, and pediococcus, as well as recently emerging vancomycin-resistant strains of enterococcus faecium and enterococcus faecalis. vancomycin, teicoplanin, and several vancomycin derivatives were tested for their activities against vancomycin-resistant gram-positive bacteria. vancomycin-resistant e. faecium and e. faecalis were generally cross-resistant to other glycopeptides, b ...	1989	2817848
in vitro selection of bacteria resistant to ly146032, a new cyclic lipopeptide.	isolates of streptococcus pneumoniae, enterococcus faecalis, enterococcus faecium, and coagulase-positive and -negative staphylococci were investigated for their abilities, in vitro, to develop resistance to ly146032. exposure of the organisms to incremental concentrations of ly146032 resulted in mics 8- to 32-fold higher than those for the original isolates. after three passages on antibiotic-free medium, the high mics were maintained for the coagulase-negative staphylococci and pneumococci, wi ...	1988	2831813
high-level resistance to gentamicin in clinical isolates of streptococcus (enterococcus) faecium.	during a 14-month period beginning in july 1986, three distinct clinical isolates of streptococcus (enterococcus) faecium demonstrating high-level resistance (mic, greater than 2,000 micrograms/ml) to gentamicin, kanamycin, tobramycin, and streptomycin were recovered from individual patients at one institution. combinations of ampicillin with any of these agents failed to show bactericidal synergism. by filter-mating techniques, high-level gentamicin resistance could be transferred into a suscep ...	1988	2847632
norfloxacin binds to human fecal material.	earlier studies have reported very high (120 to 2,700 mg/kg) concentrations of norfloxacin in feces after therapeutic doses. mics for fecal microorganisms are with few exceptions far below these levels. nevertheless, clinical investigations show that the main part of the aerobic gram-positive and the anaerobic microflora remains unaffected after norfloxacin administration. in this study, the binding of [14c]norfloxacin to fecal material was analyzed. the binding of a group of nonlabeled quinolon ...	1988	2854456
the autolytic peptidoglycan hydrolases of streptococcus faecium.	streptococcus faecium atcc 9790 possesses two peptidoglycan hydrolase activities. the first enzyme, an n-acetylmuramoylhydrolase, has been purified and has been shown to be a glucoenzyme. studies of hydrolysis of soluble, linear uncross-linked peptidoglycan chains showed that the enzyme bound strongly to the non-reducing ends of the chains and then sequentially (processively) hydrolysed susceptible bonds in that chain. the second peptidoglycan hydrolase does not appear to be a glycoprotein and d ...	1985	2860842
evaluation of the combination effects of ampicillin or vancomycin combined with streptomycin, gentamicin, tobramycin or netilmicin against enterococci.	seven strains of streptococcus faecalis, of which two possessed high-level resistance to streptomycin (mic greater than or equal to 2000 mcg/ml), and two strains of streptococcus faecium were evaluated with respect to killing-effect and duration of post-antibiotic effect (pae) of ampicillin and vancomycin in combination with streptomycin, gentamicin, tobramycin and netilmicin. no synergistic combination effects were seen with the two strains highly-resistant to streptomycin or with the two strep ...	1989	2914103
chromosome and cell wall segregation in streptococcus faecium atcc 9790.	segregation was studied by measuring the positions of autoradiographic grain clusters in chains formed from single cells containing on average less than one radiolabeled chromosome strand. the degree to which chromosomal and cell wall material cosegregated was quantified by using the methods of s. cooper and m. weinberger, dividing the number of chains labeled at the middle. this analysis indicated that in contrast to chromosomal segregation in escherichia coli and, in some studies, to that in g ...	1989	2914850
buoyant density, growth rate, and the cell cycle in streptococcus faecium.	the buoyant density in rapidly growing streptococcus faecium 9790 cells varies over the cell cycle, in contrast to the density in escherichia coli. buoyant density in s. faecium was measured by using percoll (pharmacia fine chemicals, piscataway, n.j.) density gradients. we found that the mean and coefficient of variation of the population density increased with growth rate; and within a population, the mean cell volume, which was measured electronically, increased with density. these results we ...	1989	2914870
high-level penicillin resistance among isolates of enterococci. implications for treatment of enterococcal infections.	to determine the extent and clinical significance of high-level penicillin-resistant enterococcus faecium at our institution.	1989	2923386
in-vitro evaluation of cefpirome (hr 810), teicoplanin and four other antimicrobials against enterococci.	in-vitro activities of cefpirome (hr 810), a fourth generation cephalosporin, and teicoplanin were compared with those of ampicillin, piperacillin, and vancomycin against 56 clinical isolates of enterococci. cefpirome had good activity with the mic90 and mbc90 being 4 and 16 mg/l. ampicillin and piperacillin had mbc90 of 4 and 16 mg/l. teicoplanin was extremely active with the mic90 being 1.6 mg/l while vancomycin had poor cidal activity with the mbc90 being 16 mg/l. a decrease in activity of ce ...	1985	2933380
impact of oral ciprofloxacin on the faecal flora of healthy volunteers.	the intestinal flora was studied in 12 volunteers after the administration of 400 mg ciprofloxacin b.i.d. over seven days. stool samples were taken prior to and several times after the initiation of therapy, and qualitative and quantitative analyses of microorganisms were performed. escherichia coli was eliminated in all volunteers after two days of treatment. a selection of resistant enterobacteria could not be observed. a higher incidence of streptococcus faecium was seen, mainly after the end ...	1985	2934338
colonization of infant mice by k99+ escherichia coli and lack of colonization by streptococcus faecium m74.		1985	2940835
in vitro evaluation of teicoplanin and other antimicrobials against streptococci group d bacteria.	in vitro activities of teicoplanin were compared with those of ampicillin, imipenem, rifampicin, coumermycin and vancomycin against clinical isolates of streptococcus faecalis (n = 100) and streptococcus faecium (n = 30). against str. faecalis, ampicillin and imipenem had an mic90 and mbc90 of 3.9 micrograms/ml. coumermycin and rifampicin had poor bactericidal activity with mbc90 values of 31.2 and 62.5 micrograms/ml. teicoplanin was extremely active with an mic90 of 0.95 microgram/ml and an mbc ...	1987	2962843
significance of bacterial flora in abdominal irradiation-induced inhibition of lung metastases.	we have previously reported that abdominal irradiation prior to i.v. injection of syngeneic tumor cells reduced metastases in lung. our report described an investigation of the significance of intestinal organisms in the radiation effect. we found that eliminating intestinal organisms with antibiotics totally abolished the radiation effect. monoassociation of germ-free mice revealed that the radiation effect was observable only for enterobacter cloacae, never for streptococcus faecium, bifidobac ...	1988	2966670
plasmid-mediated resistance to vancomycin and teicoplanin in enterococcus faecium.		1988	2968517
streptococcus faecalis sex pheromone (cam373) also produced by staphylococcus aureus and identification of a conjugative transposon (tn918).	streptococcus faecalis rc73 was found to harbor a conjugative plasmid (pam373) which confers a mating response to a sex pheromone (cam373) excreted by plasmid-free members of the same species. the pheromone was also detected in culture filtrates of all of 23 staphylococcus aureus strains but in only 2 of 22 coagulase negative staphylococcus strains. streptococcus sanguis challis and g9b also produced the activity, but 10 other streptococcus sanguis strains did not. the activity was also produced ...	1985	2987186
potential role of lysozyme in bactericidal activity of in vitro-acquired salivary pellicle against streptococcus faecium 9790.	the adherence of streptococcus faecium 9790 to hydroxyapatite (ha) coated with whole saliva supernatant proteins (s-ha) or parotid fluid proteins was studied. the organism was labeled with [3h]thymidine, and adherence was estimated as the radioactivity remaining associated with the variously coated ha preparations after incubation and removal of unbound microbes by washing the adherence substratum. adherence was time dependent and saturable, characteristics typical of oral streptococci in this i ...	1986	3023239
in vitro and in vivo activity of ly 146032, a new cyclic lipopeptide antibiotic.	the in vitro activity of ly 146032, a cyclic lipopeptide antibiotic belonging to the class of agents designated a21978c, was compared with those of vancomycin, cefpirome, cefotaxime, and clindamycin against selected gram-positive bacteria. the new drug inhibited all staphylococcal isolates, including methicillin-resistant strains, at concentrations of less than or equal to 1.0 microgram/ml. the activity of ly 146032 was comparable to that of vancomycin against most streptococci, but the latter d ...	1986	3024560
in vitro activity of ly146032 (daptomycin) against selected aerobic bacteria.	the in vitro activity of the new lipopeptide antibiotic ly146032 was generally four-fold greater (mic 90 less than or equal to 0.5 microgram/ml) than that of vancomycin against methicillin-susceptible or methicillin-resistant staphylococcus aureus and coagulase-negative species of staphylococcus. enterococci, streptococcus bovis, group b and viridans streptococci, and corynebacterium group j-k isolates were inhibited by less than or equal to 4 micrograms/ml of ly146032, which represented activit ...	1987	3032612
comparative in vitro activity of ly146032 (daptomycin) against gram-positive cocci.	the in vitro activity of ly146032 was compared with those of seven other antimicrobial agents against gram-positive cocci. mics of ly146032 were lowest for streptococcus pneumoniae and methicillin-susceptible staphylococcus epidermidis (0.25 mg/l). for methicillin-resistant staphylococcus epidermidis and staphylococcus aureus the mics were 1 mg/l, for enterococcus faecalis 2 mg/l and for enterococcus faecium 4 mg/l. the activity of ly146032 was in general higher than that of vancomycin. time-kil ...	1987	3032613
identification, cloning and sequence of the streptococcus faecium infb (translational initiation factor if2) gene.	the structural gene for translational initiation factor if2 (infb) from streptococcus faecium was identified by cross-hybridization with dna probes derived from the corresponding gene of bacillus stearothermophilus. the entire infb gene (ca. 2.8 kb) was cloned and sequenced. the amino acid sequence deduced from the nucleotide sequence shows that s. faecium initiation factor if2 (785 amino acids, mr 86,415) displays extensive homology (ca. 69% and 53%) with the region comprising three-quarters of ...	1988	3063954
synthesis and antifolate properties of 5,10-methylenetetrahydro-8,10-dideazaminopterin.	the synthesis of the 5,10-methylene analogue of 5,6,7,8-tetrahydro-8,10-dideazaminopterin, a potential dual inhibitor of dihydrofolate reductase (dhfr) and thymidylate synthase (ts) enzymes, is described. the dimethyl ester of 10-carboxy-4-amino-4-deoxy-8,10-dideazapteroic acid was converted to the tetrahydro derivative by hydrogenation. thermally induced cyclization of the 10-carbomethoxy and the 5-nh groups afforded the 5,10-carbonyl analogue. reduction of the lactam with borane readily yielde ...	1986	3091834
identification of poly g bound to thymidylate synthase.	thymidylate synthase activity is increased in some methotrexate-resistant strains of streptococcus faecium. the purified enzyme is associated with a polynucleotide which is not removed by dialysis. this polynucleotide contains one mole each of purine ribose and phosphate per mole base. phosphate analyses after incubation with digestive enzymes indicate a tetranucleotide with one terminal phosphate. the constituent nucleosides are recovered quantitatively in a specific assay for guanosine. on hpl ...	1986	3094514
in vitro activity of ci-934, a new quinolone antimicrobial, against gram-positive bacteria.	the in vitro activity of ci-934, a new quinolone antimicrobial, was compared with that of ciprofloxacin against selected gram-positive bacteria. concentrations of ci-934 required to inhibit 90% of strains (mic90) were twofold to eightfold lower than those of ciprofloxacin. with the exception of streptococcus faecium, all isolates were inhibited by ci-934 at concentrations less than or equal to 1.0 microgram/ml.	1986	3096629
susceptibility of enterococci. i. inhibitory and bactericidal activity of several chemoantibiotics against streptococcus faecalis and streptococcus faecium.	the authors present a microbiological study of 100 strains of enterococcus (70 strains of streptococcus faecalis and 30 strains of streptococcus faecium) tested for susceptibility to the following antibiotics, amoxicillin, ampicillin + flucloxacillin, piperacillin, rifampicin, vancomycin, netilmicin, ofloxacin, and norfloxacin. the assessment of minimum inhibitory and minimum bactericidal concentrations of these substances indicates that all have good inhibitory activity except netilmicin, which ...	1986	3098440
susceptibility of enterococci. ii. inhibitory and bactericidal activity of drugs in combination against streptococcus faecalis and streptococcus faecium.	the microbiological utility of antibiotic combinations against streptococcus faecalis and streptococcus faecium strains was studied. the drugs used were netilmicin + amoxicillin (20 strains); netilmicin + piperacillin (20 strains); netilmicin- + vancomycin (20 strains); netilmicin + rifampicin (20 strains). netilmicin used in combination with the penicillins was advantageous against streptococcus faecalis, but not uniformly against streptococcus faecium. the combinations of netilmicin with vanco ...	1986	3098441
folate analogues. 25. synthesis and biological evaluation of n10-propargylfolic acid and its reduced derivatives.	n10-propargylfolic acid (2), which is the closest pteridine analogue of the thymidylate synthase inhibitor n10-propargyl-5,8-dideazafolic acid (pddf), was synthesized starting from diethyl [p-(n-propargylamino)benzoyl]-l-glutamate (5) and n-(3-bromo-2-oxopropyl)phthalimide (8). the 7,8-dihydro derivative of propargylfolic acid served as a synthetic substrate of lactobacillus casei dihydrofolate reductase. propargylfolic acid and its reduced derivatives were weak inhibitors of l. casei thymidylat ...	1986	3100797
dispersal of a plasmid-borne chloramphenicol resistance gene in streptococcal and enterococcal plasmids.	plasmids coding for chloramphenicol resistance, five isolated from streptococci of groups a, b, and g, ten from enterococci (enterococcus faecalis, enterococcus faecium), and two from staphylococci, were tested for sequence homology with the chloramphenicol resistance gene of pip501, a 30-kb plasmid originally isolated from a group b streptococcus. the 6.3-kb hindiii fragment of pip501, known to carry the chloramphenicol resistance gene, was cloned into pbr322. a 1.6-kb portion of the cloned fra ...	1986	3101076
streptococcus faecalis sex pheromone (cad1) response: evidence that the peptide inhibitor excreted by pad1-containing cells may be plasmid determined.	streptococcus faecalis strains harboring the conjugative plasmid pad1 excrete a small peptide, iad1, which inhibits the sex pheromone cad1. studies making use of the host strain streptococcus faecium 9790, which normally does not excrete peptide pheromones, suggest that iad1 may be determined directly by pad1.	1987	3107004
[bacterial infection agents in hospitalized patients].	during six months the pattern of microbial isolates has been analysed from 1492 urinary tract infections, from 1445 wound infections, from 451 bronchial secretions, from 3067 blood cultures, from 116 peritoneal swabs and from 39 cerebrospinal fluids. all the specimens investigated were taken from patients of surgical, neurosurgical, internal wards and two intensive care units. in total, the most frequent isolates were grampositive microorganisms like staphylococus aureus, straphylococcus epiderm ...	1986	3107262
identification of streptococcus faecalis and streptococcus faecium and susceptibility studies with newly developed antimicrobial agents.	identification and susceptibility studies were performed on 301 blood and urine streptococcus faecalis and streptococcus faecium isolates. strep trio-tubes s4, s5, and s3 (carr-scarborough microbiologicals, inc.) were compared with conventional methods for accuracy and rapidity. of 282 isolates identified as s. faecalis, 98% were identified by species in 4 h with trio-tubes; the same percentage of isolates analyzed by conventional methods were identified in 24 h. all 14 s. faecium isolates (appr ...	1987	3108309
comparative survival of antibiotic-resistant and -sensitive fecal indicator bacteria in estuarine water.	the survival of antibiotic-resistant and -sensitive strains of escherichia coli, enterococcus faecalis, enterococcus faecium, streptococcus equinus, and two environmental isolates, ap17 and aq62, was examined in estuarine water. each strain was rendered resistant to a combination of two antibiotics by serial passage in increasing concentrations of antibiotics. cultures were incubated in filter-sterilized estuarine water for up to 7 days. recovery was assessed by examining colony-forming ability ...	1987	3111372
effect of acidity and antimicrobial agent-like compounds on viability of plesiomonas shigelloides.	nineteen plesiomonas shigelloides strains were evaluated for their stability at acidic and slightly alkaline phs and for their susceptibility to antimicrobial agent-like compounds produced by enteric flora. most p. shigelloides isolates were rapidly inactivated under high-acid (ph 4 or less) conditions. screening of enteric bacteria for elaboration of factors active against p. shigelloides revealed two organisms (pseudomonas aeruginosa and streptococcus [enterococcus] faecium) capable of secreti ...	1987	3112177
[a case of septicemia due to enterococcus faecium in an infant].		1987	3112279