Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. | the resistance of 330 coagulase-negative staphylococci (cns) associated with food or used in starter cultures and belonging to the species staphylococcus carnosus, staphylococcus condimenti, staphylococcus piscifermentans, staphylococcus equorum, staphylococcus succinus and staphylococcus xylosus, against 21 antibiotics was determined using the disk diffusion method. the incidence and number of resistances was found to be species and source of isolation dependent. most strains of s. equorum (63% ... | 2008 | 18625535 |
| lambdasa2 prophage endolysin requires cpl-7-binding domains and amidase-5 domain for antimicrobial lysis of streptococci. | streptococcal pathogens contribute to a wide variety of human and livestock diseases. the routine use of antibiotics to battle these pathogens has produced a new class of multidrug-resistant streptococci. thus, there is a need for new antimicrobials. bacteriophage endolysins (peptidoglycan hydrolases) comprise one group of new antimicrobials that are reportedly refractory to resistance development. the lambdasa2 prophage endolysin gene was recently isolated from a group b streptococcal genome, e ... | 2008 | 18673393 |
| co-metabolism of 2,4-dichlorophenol and 4-cl-m-cresol in the presence of glucose as an easily assimilated carbon source by staphylococcus xylosus. | comparison of the ability of staphylococcus xylosus to degrade 2,4-dichlorophenol and 4-cl-m-cresol in separate cultures is reported. bacterial adaptation and the continuous presence of glucose, as a conventional carbon source, were found to stimulate the degrading efficiency of s. xylosus. 4-cl-m-cresol exhibited higher substrate-induced toxicity with k(ig) value at 0.25 mm, comparing to 2,4-dichlorophenol (k(ig) value at 0.90 mm) at initial concentration ranging from 0.1 to 0.5 mm. degradation ... | 2009 | 18678444 |
| characterization of toxin production of coagulase-negative staphylococci isolated from food and starter cultures. | in this study a comprehensive analysis of toxin production of food associated coagulase-negative staphylococci (cns) was investigated. the strains belong to the following staphylococcal species, staphylococcus carnosus, staphylococcus condimenti, staphylococcus equorum, staphylococcus piscifermentans, staphylococcus succinus, and staphylococcus xylosus, which were isolated from fermented food and starter cultures. a collection of 330 strains were analyzed with respect to their hemolytic activity ... | 2008 | 18752861 |
| iram-an alpha2-macroglobulin from the hard tick ixodes ricinus: characterization and function in phagocytosis of a potential pathogen chryseobacterium indologenes. | the universal protease inhibitors of the alpha(2)-macroglobulin (alpha(2)m) family are evolutionarily conserved constituents of innate immunity, presumably because they guard organisms against undesired proteolytic attacks of a different origin. here, we determined the primary structure of alpha(2)-macroglobulin from the hard tick ixodes ricinus (iram) by sequencing of overlapping pcr products. predicted disulfide and glycosylation patterns, post-translational cleavage and alternative splicing w ... | 2009 | 18948134 |
| prevalence and persistence of coagulase-negative staphylococcus species in three dairy research herds. | coagulase-negative staphylococcus species (cns) were isolated from 11.3% (1407 of 12,412) of mammary quarter milk samples obtained from cows in three dairy research herds in 2005. approximately 27% (383/1407) of cns was identified to the species level. the species distribution among those cns identified from all herds was staphylococcus chromogenes (48%), staphylococcus hyicus (26%), staphylococcus epidermidis (10%), staphylococcus simulans (7%), staphylococcus warneri (2%), staphylococcus homin ... | 2009 | 18950962 |
| prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in dutch dairy herds. | in this study, the prevalence of intramammary infection (imi) with coagulase-negative staphylococci (cns) in the netherlands was estimated on 49 randomly selected herds with at least 40 lactating cows. in total, 4220 quarter milk samples were collected. the prevalence of cns imi in the netherlands was estimated at 10.8% at quarter level and 34.4% at cow level, making it the most frequently isolated group of pathogens. fourteen species of cns were identified; the most frequently isolated species ... | 2009 | 18977613 |
| exposure to culturable airborne bioaerosols during noodle manufacturing in central taiwan. | biological hazards associated with the manufacturing of noodles have not been well characterized in taiwan. this is an issue that flour workers can be exposed to bioaerosols (airborne fungi and bacteria) resulting flour-induced occupational asthma or allergic diseases. this study is to survey the species and concentrations of bioaerosols at different sites within a noodle factory for one year, and to investigate the effects of environmental factors on concentrations of bioaerosols. air samples w ... | 2009 | 19062076 |
| performance of api staph id 32 and staph-zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. | in this study, the accuracy of two phenotypic tests, api staph id 32 and staph-zym, was determined for identification of coagulase-negative staphylococci (cns) from bovine milk samples in comparison with identification based on dna-sequencing. a total of 172 cns isolated from bovine milk were classified into 17 species. the most frequently isolated species based on rpob sequencing were staphylococcus chromogenes and staphylococcus epidermidis, followed by staphylococcus xylosus, staphylococcus w ... | 2009 | 19110383 |
| culture of staphylococcus xylosus in fish processing by-product-based media for lipase production. | the objective of this study was to demonstrate that fish-processing by-products could be used as sole raw material to sustain the growth of staphylococcus xylosus for lipase production. | 2008 | 19120924 |
| effects of lactobacillus plantarum and staphylococcus xylosus on the quality characteristics of dry fermented sausage "sucuk". | the effect of starter culture, containing the strains lactobacillus plantarum gm77 and staphylococcus xylosus gm92, isolated from traditional sucuk, on the quality characteristics of dry fermented sausage (sucuk) during ripening period was studied. the microbiological (lactic acid bacteria, micrococcus/staphylococcus, enterobacteriaceae) and physicochemical (ph, a(w), npn, tbars) properties and volatile compounds, extracted by solid phase microextraction and analyzed by gas chromatography-mass s ... | 2009 | 19200122 |
| response to somatic cell count-based selection for mastitis resistance in a divergent selection experiment in sheep. | a divergent selection experiment in sheep was implemented to study the consequences of log-transformed somatic cell score (scs)-based selection on resistance to natural intramammary infections. using dams and progeny-tested rams selected for extreme breeding values for scs, we created 2 groups of ewes with a strong divergence in scs of approximately 3 genetic standard deviations. a survey of 84 first-lactation ewes of both the high and low scs lines indicated favorable responses to scs-based sel ... | 2009 | 19233814 |
| kinetics of growth and 3-methyl-1-butanol production by meat-borne, coagulase-negative staphylococci in view of sausage fermentation. | five species of meat-borne, coagulase-negative staphylococci were screened for their in vitro production of 3-methyl-1-butanol. the highest production level was encountered for staphylococcus sciuri alphasg2, despite its poor growth. with respect to staphylococcus species that are generally applied in sausage starter cultures, production of 3-methyl-1-butanol was higher with staphylococcus xylosus 3pa6 than with staphylococcus carnosus 833. mathematical modelling was used to link the kinetics of ... | 2009 | 19251335 |
| comparative subproteome analyses of planktonic and sessile staphylococcus xylosus c2a: new insight in cell physiology of a coagulase-negative staphylococcus in biofilm. | staphylococcus xylosus is a gram-positive bacterium found on the skin of mammals and frequently isolated from food plants and fermented cheese or meat. to gain further insight in protein determinants involved in biofilm formation by this coagulase-negative staphylococcus, a comparative proteomic analysis between planktonic and sessile cells was performed. with the use of a protocol previously developed, protein patterns of the cytoplasmic and cell envelope fractions were compared by 2-de. follow ... | 2009 | 19253936 |
| [isolation and study of perspective probiotic strain of spore-forming bacteria from bacillus genus]. | to study physiologic, biochemical as well as antagonistic characteristics of isolated strain of bacillus pumilus in comparison with known spore-forming bacteria from b. subtilis species. | 2009 | 19623717 |
| the uropathogenic species staphylococcus saprophyticus tolerates a high concentration of d-serine. | human urine contains a relatively high concentration of d-serine, which is toxic to several nonuropathogenic bacteria, but can be utilized or detoxified by uropathogenic escherichia coli (upec). the sequenced genome of uropathogenic staphylococcus saprophyticus contains a gene with homology to the d-serine deaminase gene (dsda) of upec. we found the gene in several clinical isolates of s. saprophyticus; however, the gene was absent in staphylococcus xylosus and staphylococcus cohnii, phylogeneti ... | 2009 | 19674114 |
| investigation of associations of yarrowia lipolytica, staphylococcus xylosus, and lactococcus lactis in culture as a first step in microbial interaction analysis. | the interactions that may occur between microorganisms in different ecosystems have not been adequately studied yet. we investigated yeast-bacterium interactions in a synthetic medium using different culture associations involving the yeast yarrowia lipolytica 1e07 and two bacteria, staphylococcus xylosus c2a and lactococcus lactis ld61. the growth and biochemical characteristics of each microorganism in the different culture associations were studied. the expression of genes related to glucose, ... | 2009 | 19684166 |
| characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (lepidoptera: noctuidae). | the characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (anticarsia gemmatalis) were the objectives of this study. twelve aerobic and anaerobic isolates of proteolytic bacteria were obtained from the caterpillar gut in calcium caseinate agar. the number of colony forming units (cfus) of proteolytic bacteria was higher when the bacteria were extracted from caterpillars reared on artificial diet rather than on soybean leaves (1.73 +/- 0.35 x 10(3) ... | 2009 | 19689886 |
| response surface optimization of medium components for naringinase production from staphylococcus xylosus mak2. | response surface methodology was used to optimize the fermentation medium for enhancing naringinase production by staphylococcus xylosus. the first step of this process involved the individual adjustment and optimization of various medium components at shake flask level. sources of carbon (sucrose) and nitrogen (sodium nitrate), as well as an inducer (naringin) and ph levels were all found to be the important factors significantly affecting naringinase production. in the second step, a 22 full f ... | 2010 | 19763896 |
| heterologous expression and purification of a heat-tolerant staphylococcus xylosus lipase. | staphylococcus xylosus is a microorganism involved in fermentation of meat products and also a natural producer of extracellular lipases. the aim of the present work was to clone and express in e. coli a lipase from s. xylosus (af208229). this lipase gene (1084 bp) was amplified from a s. xylosus strain isolated from naturally fermented salami and introduced in pet14b expression vector in order to express the recombinant fusion protein (histidine-tagged lipase) in e. coli. recombinant histidine- ... | 2010 | 19876776 |
| diversity of staphylococcus species strains based on partial kat (catalase) gene sequences and design of a pcr-restriction fragment length polymorphism assay for identification and differentiation of coagulase-positive species (s. aureus, s. delphini, s. hyicus, s. intermedius, s. pseudintermedius, and s. schleiferi subsp. coagulans). | a set of degenerate pcr primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal strains. in some strains of staphylococcus xylosus, s. saprophyticus, and s. equorum, two catalase genes, kata and katb, were found. a phylogenetic tree was generated and showed diversities among 66 partial (about 900-bp) staphylococcal kat nucleotide sequences (including 17 sequences found in genbank) representing 26 different species. the topology of ... | 2010 | 19889901 |
| biodiversity of indigenous staphylococci of naturally fermented dry sausages and manufacturing environments of small-scale processing units. | the staphylococcal community of the environments of nine french small-scale processing units and their naturally fermented meat products was identified by analyzing 676 isolates. fifteen species were accurately identified using validated molecular methods. the three prevalent species were staphylococcus equorum (58.4%), staphylococcus saprophyticus (15.7%) and staphylococcus xylosus (9.3%). s. equorum was isolated in all the processing units in similar proportion in meat and environmental sample ... | 2010 | 20141949 |
| differential characterization of biogenic amine-producing bacteria involved in food poisoning using maldi-tof mass fingerprinting. | histamine poisoning is caused by the consumption of fish and other foods that harbor bacteria possessing histidine decarboxylase activity. with the aim of preventing histamine formation, highly specific mass spectral fingerprints were obtained from the 16 major biogenic amine-producing enteric and marine bacteria by means of maldi-tof ms analysis. all bacterial strains analyzed exhibited specific spectral fingerprints that enabled its unambiguous differentiation. this technique also identified p ... | 2010 | 20151397 |
| distribution of aminogenic activity among potential autochthonous starter cultures for dry fermented sausages. | any bacterial strain to be used as starter culture should have suitable characteristics, including a lack of amino acid decarboxylase activity. in this study, the decarboxylase activity of 76 bacterial strains, including lactic acid bacteria and gram-positive, catalase-positive cocci, was investigated. these strains were previously isolated from european traditional fermented sausages to develop autochthonous starter cultures. of all the strains tested, 48% of the lactic acid bacteria strains an ... | 2010 | 20202339 |
| biochemical and structural characterization of two site-directed mutants of staphylococcus xylosus lipase. | staphylococcus xylosus af208229 lipase was expressed in e. coli containing an histidine-tag (wt-val). in the present work, in order to check the importance of the residue 309 in the specific activity, the amino acid side chain residue valine 309 was substituted by aspartate or lysine through site-directed mutagenesis. both mutant lipases (mut-lys and mut-asp) were expressed in e. coli and the recombinant histidine-tagged lipases were purified by immobilized metal ion affinity chromatography. the ... | 2010 | 20387014 |
| comparative studies on the degradation of three aromatic compounds by pseudomonas sp. and staphylococcus xylosus. | biological methods of wastewater treatment have been proved very effective for bioremediation of polluted sites. in this study, the degrading abilities of two bacteria pseudomonas sp. and staphylococcus xylosus, towards 1,2-dichlorobenzene (1,2-dcb), 2,4-dichlorophenol (2,4-dcp) and 4-cl-m-cresol, are compared. culture history and the presence of glucose as carbon source have been used for the optimization of cell's performance. 1,2-dcb showed the higher values of effective concentration (ec(50) ... | 2010 | 20486010 |
| polymerase chain reaction detection of enterotoxins genes in coagulase-negative staphylococci isolated from brazilian minas cheese. | for a long time, staphylococcus aureus has been always thought to be the only pathogenic species among staphylococcus, while coagulase-negative staphylococci (cns) were classified as contaminant agents. however, molecular techniques have shown that these microorganisms also possess enterotoxin-encoding genes. the aim of this study was to analyze the frequency of genes for staphylococcal enterotoxins sea, seb, sec, and sed in cns strains isolated from minas soft cheese and to assess the in vitro ... | 2010 | 20528174 |
| comparison of phenotypic methods in predicting methicillin resistance in coagulase-negative staphylococcus (cons) from animals. | phenotypic detection of methicillin resistance in coagulase-negative staphylococcus (cons) of animal origin has been challenging due to the heterogeneous expression of meca. to compare different phenotypic methods in predicting the meca presence in cons, a total of 87 cons isolates from agricultural animals were analyzed in this study by agar dilution, disk diffusion, and broth microdilution. meca was present in 81 cons isolates. broth microdilution demonstrated the highest sensitivity of 100% i ... | 2011 | 20538309 |
| generation of chitin-derived oligosaccharides toxic to pathogenic bacteria using chia74, an endochitinase native to bacillus thuringiensis. | to demonstrate that an endochitinase (chia74) native to bacillus thuringiensis can be used to generate chitin-derived oligosaccharides (ogs) with antibacterial activity against a number of aetiological agents of disease, including bacteria that cause diarrhoeal and emetic syndromes in humans. | 2010 | 20557451 |
| activity of telavancin and comparator antimicrobial agents tested against staphylococcus spp. isolated from hospitalised patients in europe (2007-2008). | the activity of telavancin was evaluated against staphylococcus spp. collected from european hospitals as part of an international surveillance study (2007-2008). a total of 7534 staphylococcal clinical isolates [5726 staphylococcus aureus and 1808 coagulase-negative staphylococci (cons)] were included. isolates were tested for susceptibility according to reference methods and minimum inhibitory concentration (mic) values were interpreted based on clinical and laboratory standards institute (cls ... | 2010 | 20598860 |
| aerobic bacteria from mucous membranes, ear canals, and skin wounds of feral cats in grenada, and the antimicrobial drug susceptibility of major isolates. | in a 2-year period 54 feral cats were captured in grenada, west indies, and a total of 383 samples consisting of swabs from rectum, vagina, ears, eyes, mouth, nose and wounds/abscesses, were cultured for aerobic bacteria and campylobacters. a total of 251 bacterial isolates were obtained, of which 205 were identified to species level and 46 to genus level. a commercial bacterial identification system (api/biomerieux), was used for this purpose. the most common species was escherichia coli (n=60) ... | 2011 | 20627391 |
| equilibrium, thermodynamic and kinetic studies on biosorption of mn(ii) from aqueous solution by pseudomonas sp., staphylococcus xylosus and blakeslea trispora cells. | biosorption of mn(ii) from aqueous solutions using pseudomonas sp., staphylococcus xylosus and blakeslea trispora cells was investigated under various experimental conditions of ph, biomass concentration, contact time and temperature. the optimum ph value was determined to 6.0 and the optimum biomass concentration to 1.0 g l(-1) for all types of cells. mn(ii) biosorption was found to fit better to the langmuir model for pseudomonas sp. and b. trispora and to freundlich model for s. xylosus. lang ... | 2010 | 20663610 |
| volatile profile and microbiological characterization of hollow defect in dry-cured ham. | this paper describes the physicochemical and microbiological parameters and volatile profile of the muscles surrounding the coxofemoral joint which are affected with hollow defect when compared to the part of muscles nearby which did not show this defect. differences of the same parameters between both areas in dry-cured hams without this defect were also analyzed. gram-positive catalase-positive cocci were the predominant flora in the muscles affected with hollow defect, with the main species b ... | 2010 | 20674188 |
| the application of staphylococci with flavour-generating potential is affected by acidification in fermented dry sausages. | differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of staphylococcus xylosus and staphylococcus carnosus as starter cultures. overall, higher levels of 3-methyl-1-butanol and acetoin were found for s. xylosus, with some intraspecies variability. in addition, sausage fermentation parameters affected staphylococcal growth and metabolism. strong acidification, as in northern- ... | 2010 | 20688237 |
| spontaneous staphylococcus xylosus infection in mice deficient in nadph oxidase and comparison with other laboratory mouse strains. | staphylococcus xylosus typically is described as a nonpathogenic common inhabitant of rodent skin. reports of s. xylosus as a primary pathogen in human and veterinary medicine are scarce. here we report 37 cases, affecting 12 strains of laboratory mice, of spontaneous infections in which s. xylosus was isolated and considered to be the primary pathogen contributing to the death or need for euthanasia of the animal. infection with s. xylosus was the major cause of death or euthanasia in 3 strains ... | 2010 | 20819397 |
| spontaneous bacterial keratitis in cd36 knockout mice. | cd36 is a class b scavenger receptor that is constitutively expressed in the corneal epithelium and has been implicated in many homeostatic functions, including the homeostasis of the epidermal barrier. the aim of this study is to determine (1) whether cd36 is required for the maintenance of the corneal epithelial barrier to infection, and (2) whether cd36-deficient mice present with an increased susceptibility to bacterial keratitis. | 2011 | 20847111 |
| interactions between lactobacillus sakei and cnc (staphylococcus xylosus and kocuria varians) and their influence on proteolytic activity. | to evaluate interactions between lactobacillus sakei and coagulase negative cocci (cnc) (staphylococcus xylosus and kocuria varians) and to investigate the influence of these interactions on their own proteolytic activity. | 2010 | 20875035 |
| citrus peel influences the production of an extracellular naringinase by staphylococcus xylosus mak2 in a stirred tank reactor. | staphylococcus xylosus mak2, gram-positive coccus, a nonpathogenic member of the coagulase-negative staphylococcus family was isolated from soil and used to produce naringinase in a stirred tank reactor. an initial medium at ph 5.5 and a cultivation temperature of 30°c was found to be optimal for enzyme production. the addition of ca(+)² caused stimulation of enzyme activity. the effect of various physico-chemical parameters, such as ph, temperature, agitation, and inducer concentration was stud ... | 2010 | 20922381 |
| staphylococcus xylosus isolated from an otogenic brain abscess in an adolescent. | 2010 | 20969474 | |
| identification of staphylococcus spp. using (gtg)₅-pcr fingerprinting. | a group of 212 type and reference strains deposited in the czech collection of microorganisms (brno, czech republic) and covering 41 staphylococcus species comprising 21 subspecies was characterised using rep-pcr fingerprinting with the (gtg)₅ primer in order to evaluate this method for identification of staphylococci. all strains were typeable using the (gtg)₅ primer and generated pcr products ranging from 200 to 4500 bp. numerical analysis of the obtained fingerprints revealed (sub)species-spe ... | 2010 | 21095086 |
| dominant bacterial communities in the rumen of gayals (bos frontalis), yaks (bos grunniens) and yunnan yellow cattle (bos taurs) revealed by denaturing gradient gel electrophoresis. | the dominant rumen bacteria in gayals, yaks and yunnan yellow cattle were investigated using pcr-dgge approach. the analysis of dgge profiles, identification of dominant bands and phylogenetic analysis 16s rdna sequences in dgge profiles were combined to reveal the dominant bacterial communities and compared the differences between those cattle species. dgge profiles revealed that gayals had the most abundant dominant bacteria and the lowest similarity of intraspecies between individuals than ot ... | 2010 | 21140219 |
| safety-related properties of staphylococci isolated from food and food environments. | to test some safety-related properties within 321 staphylococci strains isolated from food and food environments. | 2010 | 21143714 |
| differentiation of staphylococcus spp. by high-resolution melting analysis. | high-resolution melting analysis (hrma) is a fast (post-pcr) high-throughput method to scan for sequence variations in a target gene. the aim of this study was to test the potential of hrma to distinguish particular bacterial species of the staphylococcus genus even when using a broad-range pcr within the 16s rrna gene where sequence differences are minimal. genomic dna samples isolated from 12 reference staphylococcal strains (staphylococcus aureus, staphylococcus capitis, staphylococcus caprae ... | 2010 | 21164574 |
| evaluation of different starter cultures (staphylococci plus lactic acid bacteria) in semi-ripened salami stuffed in swine gut. | the ripening properties were evaluated in semi-ripened pork salami started by different cultures: cxp (pediococcus pentosaceus, staphylococcus xylosus and staphylococcus carnosus); rap (lactobacillus plantarum, lactobacillus sakei, s. xylosus and s. carnosus); and gy2 (l. sakei, s. xylosus and s. carnosus). these starters were chosen by their technological activities: cxp (rapid acidifying); rap (medium acidifying); and gy2 (slow acidifying plus intense reddening-flavouring). salami was enhanced ... | 2010 | 21177044 |
| the importance of staphylococci and threshold value of somatic cell count for diagnosis of sub-clinical mastitis in pirlak sheep at mid-lactation. | this study investigated the bacterial agents causing sub-clinical mastitis and the mean somatic cell counts (scc) of milk in pirlak sheep at mid-lactation. the percentage of infected udder halves was 11.4% (53/464). the most frequently isolated species were coagulase-negative staphylococci (cns) (64.2%), followed by staphylococcus aureus (24.5%) and escherichia coli (11.3%). among the cns, the most common species was staphylococcus epidermidis (38.2%). the other species isolated from milk sample ... | 2011 | 21366722 |
| identification and characterization of potential autochthonous starter cultures from a croatian "brand" product "slavonski kulen". | the microbial population of a traditional croatian fermented sausage "slavonski kulen" was isolated, identified and subjected to technological and functional characterization in order to select potential autochthonous functional starter cultures. dominant microflora were lactic acid bacteria (lab), followed by staphylococci. identification of isolated lactobacilli showed domination of leuconostoc mesenteroides and lactobacillus acidophilus while staphylococcus xylosus and staphylococcus warneri ... | 2011 | 21377808 |
| some coagulase-negative staphylococcus species affect udder health more than others. | a longitudinal study in 3 dairy herds was conducted to profile the distribution of coagulase-negative staphylococcus (cns) species causing bovine intramammary infection (imi) using molecular identification and to gain more insight in the pathogenic potential of cns as a group and of the most prevalent species causing imi. monthly milk samples from 25 cows in each herd as well as samples from clinical mastitis were collected over a 13-mo period. coagulase-negative staphylococci were identified to ... | 2011 | 21524522 |
| biodiversity and dynamics of meat fermentations: the contribution of molecular methods for a better comprehension of a complex ecosystem. | the ecology of fermented sausages is complex and includes different species and strains of bacteria, yeasts and molds. the developments in the field of molecular biology, allowed for new methods to become available, which could be applied to better understand dynamics and diversity of the microorganisms involved in the production of sausages. methods, such as denaturing gradient gel electrophoresis (dgge), employed as a culture-independent approach, allow to define the microbial dynamics during ... | 2011 | 21555189 |
| structural stability of staphylococcus xylosus lipase is modulated by zn(2+) ions. | lipases are well-known enzymes extensively used in industrial biotransformation processes. besides, their structural and catalytic characteristics have attracted increasing attention of several industries in the last years. in this work, we used biophysical and molecular modeling tools to assess structural properties of staphylococcus xylosus lipase (sxl). we studied the thermal unfolding of this protein and its zinc-dependent thermotolerance. we demonstrated that sxl is able to be active and st ... | 2011 | 21621655 |
| use of reca gene sequence analysis for the identification of staphylococcus equorum strains predominant on dry-cured hams. | spanish dry-cured ham is an uncooked meat product highly appreciated due to its characteristics flavour. in this study, we examined the accuracy of biochemical tests and 16s rdna sequencing in the identification of 56 staphylococcal strains isolated during industrial spanish dry-cured ham processes. important differences were observed comparing genotypic and phenotypic data. staphylococcus xylosus was the prevalent species identified by biochemical methods (87.5%), however, sequencing of the 16s ... | 2011 | 21645821 |
| molecular detection of enterotoxins e, g, h and i in staphylococcus aureus and coagulase-negative staphylococci isolated from clinical samples of newborns in brazil. | aims:ôçé the objective of this study was to investigate the detection of see, seg, seh and sei in strains of staphylococcus aureus and coagulase-negative staphylococci (cns) using rt-pcr. methods and results:ôçé in this study, 90 staph.┬áaureus strains and 90 cns strains were analysed by pcr for the detection of genes encoding staphylococcal enterotoxins (se) e, g, h and i. one or more genes were detected in 54 (60%) staph.┬áaureus isolates and in 29 (32┬à2%) cns isolates. staphylococcus epiderm ... | 2011 | 21672099 |
| aroma components from dried sausages fermented with staphylococcus xylosus. | sausages with and without staphylococcus xylosus were manufactured with four replicates. antibiotics and a fungicide to inhibit growth of naturally occuring microorganisms were added to the control sausages. the volatile compounds from the sausages were collected and identified by gas chromatography/mass spectrometry, the effluent evaluated by sniffing and the sausages assessed by a trained panel. the study showed that sausages fermented with staphylococcus xylosus contained several fragrant est ... | 1994 | 22059607 |
| lipolysis, proteolysis and formation of volatile components during ripening of a fermented sausage with pediococcus pentosaceus and staphylococcus xylosus as starter cultures. | bacterial growth, formation of acids, lipolysis, proteolysis, fat oxidation, formation of volatile compounds and flavour characteristics were followed during ripening and storage of a fermented sausage. the starter culture used was composed of pediococcus pentosaceus and staphylococcus xylosus. the number of pediococcus sp. increased by 1.5 log cfu/g during the first day of processing and remained constant at this level for 3 weeks. the corresponding initial increase in the numbers of staphyloco ... | 1994 | 22059658 |
| dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part i. chemical and bacteriological data. | sausages with added staphylococcus xylosus were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the numbers of surviving staphylococcus xylosus, lactic acid bacteria, ph, free fatty acids and residual amounts of nitrite and nitrate were measured. the effects of temperature and different ingredients on the chemical and bacterial data were tested using multiple line ... | 1995 | 22060168 |
| dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part ii. volatile components. | sausages, with added staphylococcus xylosus, were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the volatile compounds from the sausages were collected by dynamic headspace sampling and quantified and identified by gas chromatography and gas chromatography-mass spectrometry. the effects of temperature and different ingredients on the levels of individual volatil ... | 1995 | 22060169 |
| dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels - part iii. sensory evaluation. | sausages with added staphylococcus xylosus were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six factor fractional design. the odour of the sausages was evaluated by a quantitative descriptive method with ten descriptors and by gas chromatography olfactometry. the sensory profile was correlated to the experimental design and the volatile compounds by partial least squares analysis. also, th ... | 1995 | 22060170 |
| effect of protective cultures and packaging film permeability on shelf-life of sliced vacuum-packed cooked ham. | cooked ham produced with lactobacillus alimentarius and staphylococcus xylosus as protective cultures, and control ham were sliced, vacuum-packed in pouches with oxygen transmission rates (otr) of 360 and 77 cm(3)/m(2)/24 hr/atm, and stored at 4 °c in the dark. the addition of protective cultures increased (p < 0.05) lactic acid bacteria and micrococci and staphylococci counts in the meats. after cooking, the population of lactic acid bacteria was higher (p < 0.05) in hams produced with l. alime ... | 1996 | 22060780 |
| use of staphylococcus xylosus as a starter culture in dried sausages: effect on the biogenic amine content. | the main objective of this work was to investigate if the use of staphylococcus xylosus s81 as a starter culture in sausage production can influence the amount of histamine during ripening, and the concentration of the other most important biogenic amines, by influencing the activity of the microbial amino oxidases as well as the aminoacid decarboxylases. the results confirm that the biogenic amines presence in foods is the consequence of a complex equilibrium between the composition of the medi ... | 2002 | 22060850 |
| generation of flavour compounds in fermented sausages-the influence of curing ingredients, staphylococcus starter culture and ripening time. | the volatile profiles of fermented sausages made with either staphylococcus xylosus or staphylococcus carnosus starter cultures were studied with regard to the influence of salt concentration, ripening time and three different combinations of curing ingredients-nitrate, nitrite or nitrite/ascorbate. emphasis was laid on volatile compounds originating from degradation of branched-chain amino acids. volatile compounds were collected using dynamic headspace sampling and were identified by gas chrom ... | 2004 | 22060878 |
| effect of the fungal protease epg222 on the sensory characteristics of dry fermented sausage "salchichón" ripened with commercial starter cultures. | the effect of the addition of the fungal protease epg222 on the sensory characteristics of dry fermented sausage "salchichón" ripened with commercial starter cultures was investigated. sausages were prepared with purified epg222 and staphylococcus carnosus, staphylococcus xylosus, and lactobacillus sakei as starter cultures, ripened for 145 days and compared with a control batch only inoculated with the starter cultures. dry fermented sausages ripened with epg222 and starter cultures showed high ... | 2004 | 22061525 |
| characterization and technological properties of staphylococcus xylosus strains isolated from a tunisian traditional salted meat. | the technological properties of strains of staphylococcus xylosus were studied to select the most suitable for use as starter cultures for the production of dried fermented meat products. strains of s. xylosus were isolated from traditional salted tunisian meat and were identified by biochemical and molecular methods. thirty strains of s. xylosus were studied to evaluate their catalase, nitrate reductase, lipolytic, proteolytic and antibacterial activities as well as growth ability at different ... | 2007 | 22061592 |
| effects of starter cultures and nitrite levels on formation of biogenic amines in sucuk. | the effects of starter cultures, starter a (lactobacillus sakei+staphylococcus carnosus) and starter b (pediococcus acidilactici+staphylococcus xylosus+lactobacillus curvatus), nitrite levels (0, 75, and 150ppm) and ripening period on biogenic amine production were investigated in sucuk (turkish dry-fermented sausage) production. changes in biogenic amines, ph, water activity, moisture, non-protein nitrogen, nitrite and counts of lactic acid bacteria, micrococcus/staphylococcus, enterobacteriace ... | 2007 | 22061796 |
| effect of starter culture, spice mix and storage time and temperature on biogenic amine content of dry fermented sausages. | two types of dry fermented sausage differing in spicing mixture and the diameter (low content of red pepper+diameter 80 mm, h-sausage; high content of red pepper+diameter 55 mm, p-sausage, respectively) were produced in parallel with two different starter cultures (pediococcus pentosaceus+staphylococcus carnosus, b-samples and s. carnosus+staphylococcus xylosus+lactobacillus farciminis, f-samples, respectively). the sausages were ripened 21 days and subsequently stored 91 days at the room temper ... | 2004 | 22061810 |
| dried sausages fermented with staphylococcus xylosus at different temperatures and with different ingredient levels. part iv. amino acid profile. | sausages with added staphylococcus xylosus were fermented at different temperatures and with different added levels of salt, glucose, nitrite, nitrate and pediococcus pentosaceus in accordance with a six-factor fractional design. the amounts of individual amino acids were measured and the effects of temperature and different ingredients on the amino acid profile were tested using multiple linear regression and analysis of variance. also, the amino acid profile was correlated to the level of vola ... | 1997 | 22061849 |
| a novel polymerase chain reaction (pcr) - denaturing gradient gel electrophoresis (dgge) for the identification of micrococcaceae strains involved in meat fermentations. its application to naturally fermented italian sausages. | a new molecular method consisting of polymerase chain reaction (pcr) amplification and denaturing gradient gel electrophoresis (dgge) of a small fragment from the 16s rrna gene identified the micrococcaceae strains isolated from natural fermented italian sausages. lactic acid bacteria, total aerobic mesophilic flora, enterobacteriaceae and faecal enterococci were also monitored. micrococcaceaea control strains from international collections were used to optimise the method and 90 strains, isolat ... | 2001 | 22061920 |
| behaviour of the constitutive biota of two types of spanish dry-sausages ripened in a pilot-scale chamber. | the behaviour of the constitutive biota in eighty four samples belonging to two different types of spanish dry-cured sausages during the ripening process in a pilot-scale chamber was investigated. samples were analyzed in three stages during production: fresh product, first drying stage and finished product. lactic acid bacteria (lab) and coagulase-negative cocci (cnc) were identified by the api system. in general, evolution of lab and cnc during the ripening process of spanish dry-cured sausage ... | 2006 | 22062067 |
| microbial succession during ripening of naples-type salami, a southern italian fermented sausage. | studies were carried out on the microbiological and physico-chemical changes which occurred during the ripening of five batches of naples-type salami, manufactured without starter cultures. salami were sampled internally and externally, and the following microbial groups were studied: lactic acid bacteria, micrococcaceae and yeasts. the results obtained indicated that lactobacilli constituted the predominant flora, both on the surface and in the interior of the pieces throughout the ripening per ... | 2000 | 22062161 |
| effect of ascorbate, nitrate and nitrite on the amount of flavour compounds produced from leucine by staphylococcus xylosus and staphylococcus carnosus. | resting cells of staphylococcus xylosus and s. carnosus were incubated with ascorbate, nitrate and nitrite in defined reaction medium and their degradation of (3)h-labelled leucine into methyl-branched catabolites were studied using hplc/radiometric detection. the experiments were carried out with and without addition of a-ketoglutarate. the main catabolic product of leucine degradation was 3-methylbutanoic acid but also small amounts of a-hydroxy isocaproic acid were produced. nitrite addition ... | 2004 | 22062228 |
| characterization of staphylococcus xylosus and staphylococcus carnosus isolated from slovak meat products. | the aims of this study were to isolate, identify and characterize the population of coagulase-negative staphylococci in different types of slovak traditional sausages and to determine the metabolic properties of selected staphylococcus xylosus and s. carnosus strains for the selection of potential starter cultures to use in the processing of sausages. the strains were tested for lactic acid production, survival in the presence of bile and sensitivity to antibiotics. bacteriocin production, adhes ... | 2006 | 22062552 |
| the influence of manufacturing and drying conditions on the survival and toxinogenesis of staphylococcus aureus in two spanish dry sausages (chorizo and salchichón). | the effects of formulation, starter culture and fermentation temperature on growth and synthesis of toxin a (sea) and tnase by staphylococcus aureus during fermentation and drying of spanish chorizo were investigated. inhibitory factors able to inhibit sea synthesis in culture media were unable to prevent sea production in chorizo fermented at 20 and 30°c, though a lower temperature and starter culture sp318 (lactobacillus sake, pediococcus pentosaceous and staphylococcus xylosus) decreased stap ... | 1999 | 22062705 |
| comparison of the compositional, microbiological, biochemical and volatile profile characteristics of three italian pdo fermented sausages. | three italian pdo fermented sausages, varzi, brianza and piacentino, were compared for compositional, microbiological, biochemical and volatile profile characteristics. mean values for the gross composition varied especially due to moisture, fat, total protein and nitrate concentration which reflected differences in the ingredients and some technological parameters. cell numbers of the major microbial groups were almost similar among sausages. the major differences were found for brochothrix the ... | 2008 | 22062750 |
| characterization of the microbial flora from a traditional greek fermented sausage. | the microbial flora of naturally fermented sausages was studied. lactic acid bacteria were the dominant species at the end of fermentation in all 3 batches (ca. 10(8) cfu g(-1)). enterobacteria, pseudomonas, yeasts and aerobic spore-formers decreased during fermentation and the ripening process and were below the detection limit in the end product. enterococci exceeded 10(4)-10(5) cfu g(-1) during fermentation and remained constant at this level during ripening. gram-positive, catalase-positive ... | 2005 | 22062823 |
| molecular and technological characterization of staphylococcus xylosus isolated from naturally fermented italian sausages by rapd, rep-pcr and sau-pcr analysis. | coagulase-negative cocci (cnc) are important microorganisms in fermented sausages because they release lipases and proteases that are able to free short-chain fatty acids and peptides and aminoacids, respectively, that are responsible for the aroma of fermented sausage. the purpose of this study was to characterize staphylococcus xylosus strains isolated from naturally fermented sausages, produced in three different processing plants in the friuli venezia giulia region in the northeast of italy. ... | 2006 | 22062837 |
| the effects of a starter culture on the formation of biogenic amines in turkish soudjoucks. | the effects of starter culture containing lactobacillus sake, pediococcus pentosaceus, staphylococcus carnosus plus staphylococcus xylosus on the formation of biogenic amines during ripening of turkish soudjoucks were investigated. determination of eight different biogenic amines was carried out by reverse-phase high performance liquid chromatography (hplc) using diode array detection. it was found that aerobic plate counts (apc) decreased in the samples with added starter culture. lactic acid b ... | 1999 | 22063199 |
| effect of processing methods and starter culture (staphylococcus xylosus and pediococcus pentosaceus) on proteolytic changes in turkish sausages (sucuk) during ripening and storage. | proteolytic changes in turkish sausages (sucuk) produced by two methods (heat processing and traditional) were determined during processing and storage for 90 days. the sausages were produced with or without starter culture in both methods. a mixture of staphylococcus xylosus and pediococcus pentosaceus was used as starter culture for their acidic and proteolytic characteristics. the major changes in proteolytic characteristics of sucuk took place during the fermentation stage, with an increase ... | 2008 | 22063339 |
| growth and effect of staphylococci and lactic acid bacteria on unsaturated free fatty acids. | the growth and the effects of four species of staphylococci and six lactic acid bacteria (lab) of the genus carnobacterium, lactobacillus and pediococcus on unsaturated free fatty acids were studied. the strains were grown in complex medium supplemented either with oleic, linoleic or linolenic acids. growth was followed and oxidation of the substrates measured by tbars. the strains of staphylococcus xylosus 873, 16, staphylococcus warneri 863 and staphylococcus saprophyticus grew well on all the ... | 2000 | 22063710 |
| a fermented meat model system for studies of microbial aroma formation. | a fermented meat model system was developed, by which microbial formation of volatiles could be examined. the model was evaluated against dry, fermented sausages with respect to microbial growth, ph and volatile profiles. fast and slowly acidified sausages and models were produced using the starter cultures pediococcus pentosaceus and staphylococcus xylosus. volatiles were collected and analysed by dynamic headspace sampling and gc-ms. the analysis was primarily focused on volatiles arising from ... | 2004 | 22063950 |
| characterization of micrococcaceae isolated from iberian dry-cured sausages. | the populations of micrococcaceae in different types of iberian dry-cured sausages from central-west spain were characterized and their technological and antimicrobial properties determined in order to evaluate their suitability as starter cultures in dry-cured sausage manufacture. of a total of four hundred strains isolated from two manufacturers, one hundred and sixty-six were selected to evaluate nitrate reductase, proteolytic, lipolytic, and antimicrobial activities, and growth at different ... | 2007 | 22064035 |
| assessment of high hydrostatic pressure and starter culture on the quality properties of low-acid fermented sausages. | the addition of starter culture and high pressure processing after ripening improved the microbial quality of low-acid fermented sausages (fuet and chorizo). the use of lactobacillus sakei ctc6626 and staphylococcus xylosus ctc6013 as starter culture significantly reduced enterobacteriaceae and enterococcus levels in the finished sausages. moreover, the addition of starter culture produced sausages of similar quality to traditional low-acid fermented sausages. slightly lower ph values and higher ... | 2007 | 22064190 |
| biochemical and sensory characteristics of traditional fermented sausages of vallo di diano (southern italy) as affected by the use of starter cultures. | in this study, two strains of staphylococcus xylosus isolated from traditional fermented sausages of vallo di diano (southern italy) were used in combination with an acidifying strain of lactobacillus curvatus as starter culture for the production of fermented sausages. two starter formulation were developed combining the proteolytic but not lipolytic (prt(+), lip(-)) s. xylosus cvs11 with the l. curvatus avl3 (starter s1) and the s. xylosus fvs21 (prt(-), lip(+)) with the same strain of l. curv ... | 2007 | 22064299 |
| catalase-positive cocci in fermented sausage: variability due to different pork breeds, breeding systems and sausage production technology. | the aim of this study was to compare the ecology of catalase-positive cocci (cpc) present in traditional fermented sausages produced using different breeds of pork, each of which was raised in two different environments and processed using two different technologies. semi-quantitative molecular methods were used to determine bacterial identities. almost all fermentations were characterised by a significant increase in cpc during the first few days of fermentation, reaching values of 10(5)-10(6) ... | 2012 | 22202871 |
| antimicrobial mechanism of monocaprylate. | monoglyceride esters of fatty acids occur naturally and encompass a broad spectrum of antimicrobial activity. monocaprylate is generally regarded as safe (gras) and can function both as an emulsifier and as a preservative in food. however, knowledge about its mode of action is lacking. the aim of this study was therefore to elucidate the mechanism behind monocaprylate's antimicrobial effect. the cause of cell death in escherichia coli, staphylococcus xylosus, and zygosaccharomyces bailii was inv ... | 2012 | 22344642 |
| intramammary infections and teat canal colonization with coagulase-negative staphylococci after postmilking teat disinfection: species-specific responses. | coagulase-negative staphylococci (cns) are the most common pathogens associated with intramammary infections (imi) in dairy cows. we hypothesized that postmilking teat disinfection would reduce microbial colonization of the teat canal and thus reduce the prevalence of imi caused by certain cns species. the efficacy of iodine postmilking teat dip was tested against cns colonization of the teat canal, and incidence of imi was measured. using an udder-half model, 43 holstein cows at the washington ... | 2012 | 22459837 |
| monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis. | soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ... | 2012 | 22475947 |
| reactive oxygen species produced by the nadph oxidase 2 complex in monocytes protect mice from bacterial infections. | chronic granulomatous disease (cgd) is an inherited disorder characterized by recurrent life-threatening bacterial and fungal infections. cgd results from defective production of reactive oxygen species by phagocytes caused by mutations in genes encoding the nadph oxidase 2 (nox2) complex subunits. mice with a spontaneous mutation in ncf1, which encodes the ncf1 (p47(phox)) subunit of nox2, have defective phagocyte nox2 activity. these mice occasionally develop local spontaneous infections by st ... | 2012 | 22491245 |
| investigation of antimicrobial activity and statistical optimization of bacillus subtilis spb1 biosurfactant production in solid-state fermentation. | during the last years, several applications of biosurfactants with medical purposes have been reported. biosurfactants are considered relevant molecules for applications in combating many diseases. however, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. use of inexpensive substrates can drastically decrease its production cost. here, twelve solid substrates were screened for the production of bacillus subtilis spb1 biosurfactant and t ... | 2012 | 22536017 |
| degradation of pcbs in a frankfurter-type meat emulsion: effects of a meat starter, its proteins extract and thermal treatments. | the degradation of a series of polychlorinated biphenyl (pcb) congeners (pcbs 10, 28, 52, 138, 153, 180) in meat emulsions of a frankfurter type was investigated. with a pool of these pcbs added to the meat emulsion, three initial experimental groups were used: no further addition, or plus a commercial meat starter (containing staphylococcus xylosus and staphylococcus carnosus), or plus the protein extract from this commercial meat starter. prior to further treatments, these samples were incubat ... | 2012 | 22659289 |
| synthesis and antibacterial activity of novel cationic bodipy photosensitizers. | bodipys are versatile dyes never tested before in photodynamic application against prokaryotes. we specifically synthesized two cationic bodipys (compounds 3 and 4) with structural features suitable for this pourpose. the novel bodipys are both characterized by the presence of one pyridinium cationic group on position 8 and two iodine atoms on 2,6-positions of the dipyrrolylmethene structure, thus ensuring solubility in 1/1 water/organic solvent mixture and a good singlet oxygen formation rate. ... | 2012 | 22682365 |
| quantification of bacteria on abiotic surfaces by laser scanning cytometry: an automated approach to screen the antifouling properties of new surface coatings. | bacterial biofilms are a persistent source of contamination, and much effort has been invested in developing antifouling surfaces or coatings. a bottleneck in developing such coatings is often the time-consuming task of screening and evaluating a large number of surface materials. an automated high-throughput assay is therefore needed. in this study, we present a promising technique, laser scanning cytometry (lsc), for automated quantification of bacteria on surfaces. the method was evaluated by ... | 2012 | 22713755 |
| multidrug-resistant coagulase-negative staphylococci in food animals. | to study the antimicrobial resistance of coagulase-negative staphylococci (cons) in animals. | 2012 | 22816491 |
| differences between coagulase-negative staphylococcus species in persistence and in effect on somatic cell count and milk yield in dairy goats. | coagulase-negative staphylococci (cns) are the most commonly isolated bacteria from goat milk. the goal of this study was to explore and describe differences between cns species in persistence of intramammary infection (imi) and in effect on somatic cell count (scc) and milk yield (my). milk samples were collected from 530 does from 5 dutch dairy goat herds on 3 occasions during 1 lactation. coagulase-negative staphylococci species were identified at the species level by transfer rna-intergenic ... | 2012 | 22916911 |
| formation and identification of nitrosylmyoglobin by staphylococcus xylosus in raw meat batters: a potential solution for nitrite substitution in meat products. | staphylococcus xylosus and pediococcus pentosaceus isolated from chinese dried sausage were assessed for their ability to convert metmyoglobin into nitrosylmyoglobin in mann-rogosa-sharp broth model systems and raw pork meat batters without the addition of nitrite. the results showed that samples in model systems with s. xylosus cultures had an absorption spectra that is typical of nitrosylmyoglobin, an obvious pink colour (judged by visual inspection) and a significantly higher a-value than the ... | 2013 | 22926033 |
| influence of beet sugar, calcium lactate, and staphylococcus xylosus (with nitrate reductase activity) on the chemical, microbiological, and sensorial properties of persian uncured frankfurters. | this study investigated the effects of beet sugar (bs), calcium lactate (cl), and staphylococcus xylosus (sx) on the general characteristics of uncured frankfurters. minimum residual nitrite was observed in samples with a high level of bs and s. xylosus (8log(10) l0 viable cfu/g) after 2 mo. there was an increase in total aerobic counts in frankfurters after 4 wk. samples containing higher bs and cl had no clostridium perfringens at storage time. histamine content was similar for all treatments ... | 2012 | 22946602 |
| diffuse scaling dermatitis in an athymic nude mouse. | this report describes the clinicopathological features of a case of diffuse scaling dermatitis that occurred in a 16-week-old female athymic nude (crtac:ncr-foxn1(nu)) mouse. gross presentation was suggestive of corynebacterium bovis infection (scaly skin disease). however, c. bovis was not isolated from the skin of the affected animal or from the skin of unaffected crtac:ncr-foxn1(nu) mice housed in the same cage or room. staphylococcus xylosus was instead isolated in high numbers from the skin ... | 2013 | 23064880 |
| a negative role for myd88 in the resistance to starvation as revealed in an intestinal infection of drosophila melanogaster with the gram-positive bacterium staphylococcus xylosus. | drosophila melanogaster is a useful model to investigate mucosal immunity. the immune response to intestinal infections is mediated partly by the immune deficiency (imd) pathway, which only gets activated by a type of peptidoglycan lacking in several medically important gram-positive bacterial species such as staphylococcus. thus, the intestinal host defense against such bacterial strains remains poorly known. here, we have used staphylococcus xylosus to develop a model of intestinal infections ... | 2013 | 23083631 |
| characterization of the ability of coagulase-negative staphylococci isolated from the milk of canadian farms to form biofilms. | mastitis is the most common and detrimental infection of the mammary gland in dairy cows and has a major economic impact on the production of milk and dairy products. bacterial mastitis is caused by several pathogens, and the most frequently isolated bacterial species are coagulase-negative staphylocci (cns). although cns are considered minor mastitis pathogens, the importance of cns has increased over the years. however, the mechanism and factors involved in cns intramammary infection are poorl ... | 2013 | 23141829 |
| characterization of staphylococcus xylosus isolated from broiler chicken barn bioaerosol. | in this study we isolated and characterized staphylococcus xylosus, a coagulase-negative staphylococcal species considered as commensal and one of the prevalent staphylococcal species found in poultry bioaerosol. isolates were obtained using air samplers and selective phenylethyl alcohol agar for gram-positive bacteria during 35-d periods at different times of the day. a total of 200 colonies were recovered and after basic biochemical tests were performed, presumptive staphylococci were subseque ... | 2012 | 23155006 |
| erythema nodosum associated with staphylococcus xylosus septicemia. | staphylococcus xylosus is a coagulase-negative staphylococcus. it is a commensal bacterium associated with skin and mucous membranes and occasionally it can cause human infections. we report the first case of erythema nodosum developed in a young woman with s. xylosus septicemia and specific serum antibody response. | 2012 | 23266237 |
| a staphylococcus xylosus isolate with a new mecc allotype. | recently, a novel variant of meca known as mecc (meca(lga251)) was identified in staphylococcus aureus isolates from both humans and animals. in this study, we identified a staphylococcus xylosus isolate that harbors a new allotype of the mecc gene, mecc1. whole-genome sequencing revealed that mecc1 forms part of a class e mec complex (meci-mecr1-mecc1-blaz) located at the orfx locus as part of a likely staphylococcal cassette chromosome mec element (sccmec) remnant, which also contains a number ... | 2012 | 23274660 |
| treating animal bites: susceptibility of staphylococci from oral mucosa of cats. | infected wounds determined by cats' bites represent high costs to public health, and their adequate treatment relies on the knowledge of the antimicrobial susceptibility of bacterial agents found in the oral microbiota. members of the genus staphylococcus sp. belong to the microbiota of the oral mucosa of cats and are frequently involved in secondary infections of these wounds. this study aimed to evaluate the antimicrobial susceptibility of staphylococcus species isolated from oral mucosa of ca ... | 2012 | 23280142 |