Publications

isolation and characterization of an ehrlichia sp. from a patient diagnosed with human ehrlichiosis.	a new disease was recognized in the united states in 1986. the etiologic agent, although not previously isolated from a human, appeared to be serologically related to ehrlichia canis, a canine leukotropic rickettsia. we obtained blood specimens from 27 febrile patients with a history of tick exposure. leukocytes from 24 patients not treated with tetracycline were placed onto a monolayer of dh82 cells. we performed indirect immunofluorescence on sera from all 27 febrile patients as well as sera f ...	1991	1757543
rickettsiae and borrelia burgdorferi in ixodid ticks.	nymphs and adults of hard-bodied ticks were collected in connecticut and tested by direct and indirect immunofluorescence staining methods for rickettsiae and borrelia burgdorferi. of the 609 ixodes dammini ticks examined, 59 (9.7%) harbored rickettsialike microorganisms in hemocytes (blood cells). these bacteria reacted with fluorescein-conjugated antiserum to ehrlichia canis, the etiologic agent of with fluorescein-conjugated antiserum to ehrlichia canis, the etiologic agent of canine ehrlichi ...	1991	1757551
ehrlichia chaffeensis, a new species associated with human ehrlichiosis.	the bacterial 16s rrna genes from blood samples of two patients with human ehrlichiosis and from an isolate recovered from one of the patients were amplified by using the polymerase chain reaction. the amplimers were then cloned and sequenced. the 16s rrna gene sequence was also determined for ehrlichia canis (two strains), e. equi, e. phagocytophila (two strains), and e. sennetsu (two strains). these sequences, along with a previously published 16s rrna gene sequence of e. risticii, were compar ...	1991	1757557
development of an in vitro microtest to assess drug susceptibility of babesia bovis and babesia bigemina.	continuous cultivation of the bovine hemoparasites babesia bovis and babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample. inhibitory concentrations (ic50s) of a commonly used babesicide, quinuronium sulfate, evaluated by this in vitro method were found to be 5 x 10(-8) g/ml ...	1991	1779305
oesophageal intramural pseudodiverticulosis with similar lesions in the duodenum.	oesophageal intramural pseudodiverticulosis is characterized by a segmental or diffuse distribution of numerous small outpouchings varying in size from 1 to 5 mm. review of previously described cases revealed a significant incidence of associated debilitating systemic disorders such as diabetes mellitus, malignancy, alcoholism and tuberculosis. no record could be found in the literature of a similar process occurring in other parts of the gastro-intestinal tract. a case is reported of a severe a ...	2006	1797795
amino acid sequence of crustacean hyperglycemic hormone (chh) from the crayfish, orconectes limosus: emergence of a novel neuropeptide family.	the primary structure of the major form of chh from sinus glands of the crayfish, orconectes limosus, was determined by manual edman microsequencing. it is a 72-residue peptide with a calculated mr of 8400 da. in the number of residues, it is identical to the chh of carcinus maenas and very similar to mih (moult inhibiting hormone) of homarus americanus. all three peptides have pglu as n-terminus in common, and val-nh2 is the c-terminal residue in orconectes and carcinus chh. six cys residues oc ...	2007	1800954
albumin excretion rate is not increased in atherosclerotic patients with peripheral vascular disease.	in type i diabetic patients, microalbuminuria is considered predictive of nephropathy and has been found associated with an increased mobility and mortality for atherosclerosis. an association between microalbuminuria and atherosclerosis has been reported in non diabetic atherosclerotic patients with hypertension. the aim of this study is to evaluate whether albumin excretion rate (aer) is increased in a selected group of normotensive patients with documented peripheral atherosclerotic disease. ...	2007	1821046
candida albicans-specific ly-2+ lymphocytes with cytolytic activity.	to determine whether antigen (ag)-specific cytotoxic t lymphocytes are generated during experimental candida albicans infection, purified l3t4+ and ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, c. albicans ag, and interleukin 2. yeast-infected bone marrow macrophages were used as target cells in a standard 51cr-release assay. freshly isolated l3t4+ and ly-2+ lymphocytes failed to lyse either target cell type. however, ag-specific, major histoco ...	1991	1828430
candida albicans-specific ly-2+ lymphocytes with cytolytic activity.	to determine whether antigen (ag)-specific cytotoxic t lymphocytes are generated during experimental candida albicans infection, purified l3t4+ and ly-2+ lymphocytes from immunized mice were cultured in the presence of syngeneic accessory cells, c. albicans ag, and interleukin 2. yeast-infected bone marrow macrophages were used as target cells in a standard 51cr-release assay. freshly isolated l3t4+ and ly-2+ lymphocytes failed to lyse either target cell type. however, ag-specific, major histoco ...	1991	1828430
altered coupling states between calcium transport and (ca2+, mg2+)-atpase in the as-30d ascites hepatocarcinoma plasma membrane.	plasma membrane fractions from normal, regenerating liver and the as-30d ascites hepatocarcinoma exhibited a high degree of enrichment when a set of plasma membrane enzyme markers were studied in comparison to the ones associated to the mitochondrial and cytosolic compartments. while the (ca2+, mg2+)-atpase observed for the plasma membrane fraction isolated from normal liver showed an activity of 1.2 mumoles/mg/min, the regenerating liver and the as-30d plasma membrane fractions presented a much ...	1991	1828860
the molecular basis of the hepatic microsomal glucose-6-phosphatase system.		2001	1850300
signal detectability: the use of roc curves and their analyses.	issues related to roc curves are addressed. the original article on the subject by lee lusted, describing the "state of the art" 20 years ago, is reviewed. the concepts that lusted addressed are then expanded, suggesting the current state of the art. new issues that have arisen with regard to roc curves and their use in medicine are addressed. finally, potential areas for future investigation are suggested.	2007	1865776
lovastatin inhibits gallstone formation in the cholesterol-fed prairie dog.	the efficacy of lovastatin, an inhibitor of hepatic cholesterol synthesis in the prevention of cholesterol gallstone formation, was evaluated in the prairie dog model. two groups of animals were maintained on either nonlithogenic or 1.2% cholesterol-enriched chow for 21 days. seven of the animals in each group received lovastatin, and the remaining six received only distilled water. all of the cholesterol-fed/water-treated animals had crystals and 83% had gallstones, but none of the cholesterol- ...	1991	1867522
cherry: who are you?	do we need a new role model to inspire today's teenagers to look at nursing as a career? if so, who and what should this character be? billingsley offers her ideas.	2007	1876191
what is a biological?		2005	1888493
the tribe ehrlichieae and ehrlichial diseases.	the tribe ehrlichieae consists of gram-negative minute cocci that are obligate intracellular parasites classified in the family rickettsiaceae. although ehrlichial organisms have been observed in leukocytes for many years, only a few species have been cultured in quantities sufficient for biochemical and molecular analyses. recents studies on 16s-rrna sequence analysis and energy metabolism showed that the genus ehrlichia is closely related to the genus rickettsia. there is, however, no antigeni ...	1991	1889044
changing trends in surgery for acute cholecystitis.		2001	1891945
hormonal control of gene expression: interactions between two trans-acting regulators in drosophila.	the steroid hormone 20-hydroxyecdysone (20he) and the broad-complex locus (brc) are involved in regulating developmental changes in gene expression around the time of metamorphosis in drosophila. we have investigated the regulatory interactions between 20he, brc, and a set of genes expressed in the fat body of third-instar drosophila larvae. rna levels for two hormone-inducible genes, larval serum protein-2 and p1, accumulate to normal levels in brc-mutant larvae. in contrast, rna levels for the ...	2007	1899839
neurologic manifestations of trypanosomiasis in a dog.	a 13-month-old doberman pinscher was evaluated because of slowly progressive paraparesis and signs of depression. the dog had temporal, supraspinatus, and infraspinatus muscle atrophy, bilateral enophthalmos, superficial inguinal lymphadenopathy, tachycardia with pulse deficits, and lesions of active and inactive chorioretinitis. neurologic abnormalities included hyperreflexic patellar reflexes, lack of conscious proprioception, signs of superficial pain in the hind limbs, and depressed hopping ...	1991	1899857
[carbonic anhydrase iii--a marker of the myoepithelial cells of human salivary glands].	carbonic anhydrase iii (caiii) is the isoenzyme purified from the human skeletal slow muscle immunohistochemically revealed in smooth muscle cells of uterus, myoepithelial cells of salivary, lactiferous and prostatic glands of man. immunohistochemical determination of caiii is a useful approach to correct the identification of myoepithelial cells in human salivary glands.	1991	1905166
the coexistence of lymphangiosarcoma and kaposi's sarcoma in a renal transplant recipient.	a case of coexisting lymphangiosarcoma and kaposi's sarcoma that occurred in a female renal transplant recipient is presented. both sarcomas were localized to the skin and were slowly progressive over several years. the coexistence of these two sarcomas may indicate that they arose from a common precursor endothelial cell and that systemic immune suppression may be important in the pathogenesis of both these malignancies.	2007	1913470
contamination of operating room personnel during total arthroplasty.	the authors prospectively evaluated the degree of contamination to the operating room team during 60 consecutive total joint arthroplasties. each member of the team was required to wear a hood, mask, protective eyewear, and shoecovers. at the conclusion of each procedure, all members were assessed in terms of degree and location of contamination. one hundred percent of the surgeons and first assistants were exposed. the face and eyewear were noted to be the area of greatest contamination. the au ...	2007	1914319
[autosomal recessive oculopharyngeal "muscular dystrophy"--clinical features and association with reduced activity of myophosphorylase].	we reported two cases of brothers demonstrating oculopharyngeal muscular dystrophy (opmd). the cases had consanguineous parents and five healthy siblings, which suggested the autosomal recessive inheritance. the initial symptom was slowly progressive blepharoptosis with onset in the third decade. on examination, total external ophthalmoplegia was observed in both patients. additionally, the elder, a 57-year-old man, exhibited dysarthria, dysphagia and muscular weakness with atrophy of the face, ...	1991	1914322
magnetic resonance imaging in infants exposed to cocaine prenatally: a preliminary report.		2007	1914354
canine ehrlichiosis in six dogs with persistently increased antibody titers.	chronic ehrlichiosis was diagnosed in six dogs on the basis of increased immunofluorescent antibody titers to ehrlichia canis. although clinical signs varied, all six dogs were anemic, hyperglobulinemic, and an igg monoclonal gammopathy was documented in five dogs in which serum protein electrophoreses were performed. all dogs were treated with tetracycline for at least 14 days; four dogs also received immunosuppressive drugs. clinical signs resolved within 1 week, hematologic abnormalities reso ...	1991	1920256
antigenic analysis of four species of the genus ehrlichia by use of protein immunoblot.	the antigenic profile of ehrlichia canis, e risticii, e sennetsu, and e equi was investigated by the use of protein (western) immunoblot technique. results of analysis of serum from acutely and chronically infected animals indicated that the 4 ehrlichia species share a unique 25-kd polypeptide in addition to other peptides. immune sera from dogs inoculated with e canis recognized a wide range of e canis polypeptide antigens, as determined by western blot analysis. a larger number of e sennetsu p ...	1991	1928904
parkinson's disease as community health problem: study in norwegian nursing homes. the norwegian study group of parkinson's disease in the elderly.	to examine the extent of under-diagnosis and overdiagnosis of parkinson's disease and to determine quality of treatment in a defined population.	2007	1932932
time course and interrelation of reperfusion-induced st changes and ventricular arrhythmias in acute myocardial infarction.	with the increasing use of thrombolytic therapy, the presence and time course of reperfusion-induced ventricular arrhythmias and st-segment changes have become of particular interest. technical improvements in bipolar holter monitoring offer the opportunity to record both parameters continuously and simultaneously. time course and interaction of both parameters in dependence on the onset of thrombolysis and time of reperfusion were investigated in 30 patients with acute myocardial infarction. re ...	2007	1951071
the glycemic index: methodology and clinical implications.	there is controversy regarding the clinical utility of classifying foods according to their glycemic responses by using the glycemic index (gi). part of the controversy is due to methodologic variables that can markedly affect the interpretation of glycemic responses and the gi values obtained. recent studies support the clinical utility of the gi. within limits determined by the expected gi difference and by the day-to-day variation of glycemic responses, the gi predicts the ranking of the glyc ...	2007	1951155
efficacy and pharmacokinetics of intravenous immune globulin administration to high-risk neonates.	objective--to determine whether intravenous immune globulin (ivig) administration modifies the incidence of infections in high-risk neonates. design--randomized, double-blind study. setting--neonatal intensive care unit at a tertiary care center.	2007	1951212
pyogenic meningitis and cerebral abscesses after endoscopic injection sclerotherapy.	infectious complications of endoscopic injection sclerotherapy (eis) are rare. we report here a case in which pyogenic meningitis and multiple cerebral abscesses developed after a session of eis. the patient was successfully treated with a combination of parenteral antibiotics and repeated bedside pus aspirations. prophylactic antibiotics may be considered in such situations for subsequent sessions of eis.	2007	1951249
hemophagocytic syndrome: a cause of pancytopenia in human ehrlichiosis.	a 67-year-old white man with human ehrlichiosis infection complicated by pancytopenia, hemophagocytic syndrome, disseminated intravascular coagulopathy and septic shock is presented. the patient had been on a three-week camping trip to california, colorado, utah, and new mexico. the diagnosis of human ehrlichiosis was confirmed by sixteen-fold rise in antibody titer to ehrlichia canis, and supported by the characteristic cytoplasmic inclusions. human ehrlichiosis should be considered in the diff ...	1991	1951324
[pregnancy after transfer of the gametes into the fallopian tubes].	three patients with idiopathic sterility were treated by transferring the gametes into uterine tubes. clomiphene+ citrate stimulation of superovulation was employed. preovulatory oocytes were obtained via the laparoscopic approach. two oocytes with capacitated spermatozoa were placed into the tube of patient 1, four cells into one tube of the second patient, and two cells into each tube of the third patient. pregnancy started in two cases, uterine in one, eventuating in 12-13 weeks in spontaneou ...	2007	1951975
evaluation of rhipicephalus sanguineus as a potential biologic vector of ehrlichia platys.	the brown dog tick, rhipicephalus sanguineus (acari: ixodidae), transmits several diseases among dogs including ehrlichia canis infection. the role of rhipicephalus sanguineus as a biologic vector for e platys, the rickettsial agent of infectious canine cyclic thrombocytopenia, was studied in dogs. laboratory-cultured, pathogen-free nymph ticks were fed to repletion on dogs acutely infected with e platys. tick engorgement coincided with the development of initial parasitemia and thrombocytopenia ...	1991	1952347
serologic diagnosis of human ehrlichiosis using two ehrlichia canis isolates.	ehrlichia canis or a closely related rickettsial organism has been implicated serologically and morphologically as the causative agent of human ehrlichiosis in the united states. although e. canis has been serially propagated in primary canine monocytes, only a limited quantity of antigen is obtained by this method. a continuous canine macrophage cell line, dh82, supports the growth of a new isolate of e. canis established from the whole blood of a carrier dog in oklahoma. serologic comparison o ...	1991	1995730
ehrlichial diseases of dogs.	ehrlichial diseases of dogs currently are recognized to be caused by ehrlichia canis, ehrlichia platys, ehrlichia equi, and ehrlichia risticii. information on each disease's history, transmission, pathogenesis, clinicopathologic findings, diagnosis, therapy, and prevention is presented in this article. ehrlichia canis is the most common cause of clinical illness. case reports are included to illustrate common presentations of ehrlichia canis infections.	1991	2014630
serological evaluation of ehrlichia canis infections in military dogs in africa and reunion island.	sixty-eight dogs from four african countries and reunion island were tested for antibodies against ehrlichia canis. twenty-six dogs (50%) in tunisia, senegal and chad were found positive using the indirect fluorescence antibody test. dogs from both the central african republic and reunion island were all negative. thus, this preliminary report confirms the presence of e. canis in africa. larger studies will be necessary to evaluate the current epidemiologic situation of canine ehrlichiosis in th ...	1991	2024432
[human infection by ehrlichia canis in spain?].		1991	2041392
antiarrhythmic properties of benzyl-triamterene derivatives in the coronary artery ligated and reperfused rat.	triamterene (cas 396-01-0) and a series of benzyl-triamterene derivatives were evaluated for their antiarrhythmic properties in the coronary artery ligated and reperfused (cal-r) rat. the effects were compared with the antiarrhythmic activity of the potassium sparing diuretic amiloride and drugs out of the class-i (lidocaine) and class-iii (amiodarone and sotalol). triamterene and sotalol revealed at high doses antifibrillator activity, while the benzyl-triamterenes 2, 3, 5 and 6 could also depr ...	1991	2043173
ventricular fibrillation in the wolff-parkinson-white syndrome.	ventricular fibrillation (vf) is a well-known but rare complication of the wolff-parkinson-white syndrome (wpw). clinical and electrophysiological data of 23 patients with spontaneous vf were compared with data from 100 consecutive patients with wpw without vf but with symptomatic supraventricular tachycardia. the 23 patients were collected in a multicentre retrospective study in seven european centres. vf occurred in only one patient who was receiving antiarrhythmic drugs, and was the first man ...	2007	2044547
ehrlichiosis.	ehrlichiae are tick-borne rickettsial organisms that are well known as veterinary pathogens. however, since 1986, over 100 cases of human infection by ehrlichia canis or a closely related species have been identified primarily in the southeastern and south central united states. human ehrlichiosis is characterized by high fever, rigors, headache, myalgia, anorexia, and, sometimes, gastrointestinal complaints. rash occurs in a minority of cases. commonly observed laboratory abnormalities include ...	1991	2051014
[canine ehrlichiosis in senegal: human and canine seroepidemiological survey in dakar].	since 1986, several cases of human ehrlichiosis due to ehrlichia canis have been reported in the u.s.a. suspecting a pathology transmissible from dog to man the authors conducted an epidemiologic survey in an ehrlichiosis zone in senegal on a population of 42 men and 66 dogs. in 1987, this rickettsiosis accounted for the deaths of a good half of the military dogs stationed in dakar. yet two years after implementing a prophylactic policy the seroprevalence rate in the kennel dropped from 53% to 1 ...	1991	2072850
[derivative spectroscopy and its use in sanitary hygiene control].	an analytical method is developed for defining the residual quantities of rodenticide "brodifacoum" (talon) used in the control of harmful field rodents and especially for species microtus. the method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. the second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. the peak height in this interval is in proportio ...	2007	2099444
immunohistologic demonstration of ehrlichia canis.		1990	2165753
the dose rate effects on the in vitro radiolysis products of magnesium-guanosine-5'-monophosphate complexes in aqueous solutions.	this study examined the effects on the radiolysis of magnesium-guanosine-5'-monophosphate complexes in deaerated aqueous solutions, investigated by fourier transform infrared (ft-ir) spectroscopy. it was found that when the system was irradiated with a dose rate of 500 rad/min (low dose rate), the oh radicals, h atoms and hydrated electrons (eaq-) produced from radiolysis of water reacted mainly by addition to the double bonds c4 = c5 and n7 = c8 of guanine. when the dose rate was 23,800 rad/min ...	2007	2168727
the differentiation of excitability in embryonic chick limb motoneurons.	the well-documented role of neuromuscular activity as a regulator of motoneuron and muscle development raises important questions about the differentiation of excitability in motoneurons. we have recently described changes in expression of voltage-dependent calcium currents that take place during neuromuscular development in the chick embryo (mccobb et al., 1989). we now report similar analyses, using whole-cell patch-recording methods, of the major currents underlying action potential generatio ...	2007	2168941
ehrlichiosis--a disease of animals and humans.	ehrlichiae are one of several kinds of obligate intracellular bacteria. taxonomically, they are grouped with rickettsiae, but they can be distinguished by their unique tropism for circulating leukocytes. ehrlichia canis causes a pancytopenia in dogs that becomes chronic if untreated. certain breeds develop severe infections, characterized by fever, anorexia, dramatic weight loss, marked pancytopenia, anemia, peripheral edema, and hemorrhage. ehrlichia risticii, a recently discovered species, is ...	1990	2181027
diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity.	human ehrlichiosis, an acute febrile illness caused by ehrlichia canis or a closely related rickettsial organism, was first identified in 1986. from 1986 through 1988, sera from 85 patients demonstrated a fourfold rise or fall in antibody titer to e. canis. seven (22%) of 32 patients initially tested during the first week after onset of illness. 17 (68%) of 25 tested during the second week, and all 18 tested during the third week had titers that exceeded the minimum positive titer of greater tha ...	1990	2192013
changes of ngf level in mouse hypothalamus following intermale aggressive behaviour: biological and immunohistochemical evidence.	nerve growth factor (ngf) immunoreactivity was detected in the hypothalamus of adult male mice. ngf-immunoreactive cell bodies were examined through consecutive brain sections, and it was found that most of the ngf-positive cells were located in the dorsomedial and mediolateral portions of the hypothalamus. tissue culture bioassays showed that hypothalamic extract elicits neurite outgrowth from both chick sensory ganglia and rat superior cervical ganglia neurons, and that these effects are inhib ...	1990	2202329
epidemiologic, clinical, and laboratory findings of human ehrlichiosis in the united states, 1988.	in 1988, the centers for disease control and the oklahoma state department of health identified 40 patients who had a fourfold or greater change in antibody titer in response to ehrlichia canis. the median age of these patients was 42 years, 83% were male, 76% became ill between may and july, and 92% reported recent exposures to ticks. patients resided in or were exposed to ticks in 14 states, including five where ehrlichiosis had not been reported before 1988. thirty-four patients (85%) were ho ...	1990	2214103
the occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues.	the occurrence and subcellular distribution of the non-specific lipid transfer protein (nsl-tp; sterol carrier protein 2) in rat tissues was investigated by the immunoblotting technique using the affinity purified antibody against rat liver nsl-tp. highest levels of the protein were found in the homogenates of liver, lung and adrenals, whereas it could hardly be detected in brain. in other tissues (i.e., testis, kidney, heart and intestine) the protein was present at intermediate concentrations. ...	1990	2223868
the occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues.	the occurrence and subcellular distribution of the non-specific lipid transfer protein (nsl-tp; sterol carrier protein 2) in rat tissues was investigated by the immunoblotting technique using the affinity purified antibody against rat liver nsl-tp. highest levels of the protein were found in the homogenates of liver, lung and adrenals, whereas it could hardly be detected in brain. in other tissues (i.e., testis, kidney, heart and intestine) the protein was present at intermediate concentrations. ...	1990	2223868
molecular cloning of ehrlichia risticii and development of a gene probe for the diagnosis of potomac horse fever.	a gene bank of ehrlichia risticii was constructed in plasmid vector puc13. five clones representing discrete regions of the e. risticii genome were tested for their ability to hybridize specifically to e. risticii dna. none of the clones cross-hybridized with ehrlichia equi dna, whereas four of these clones cross-hybridized with ehrlichia canis and ehrlichia sennetsu dnas. however, one clone carrying a 1-kilobase hindiii fragment of e. risticii dna failed to cross-react with the genomes of e. se ...	1990	2229378
failure to transmit ehrlichia canis (rickettsiales: ehrlichieae) with otobius megnini (acari: argasidae).	an ear tick, otobius megnini (dugès) recovered from a child who had serologic evidence of ehrlichiosis, was examined for ehrlichia species microscopically and by inoculation into a susceptible dog; no evidence of infection was found in the tick. experimental transmission of e. canis by laboratory-reared o. megnini was attempted; neither transstadial nor transovarial transmission occurred.	1990	2231616
concurrent babesiosis and ehrlichiosis in the dog: blood smear examination supplemented by the indirect fluorescent antibody test, using cowdria ruminantium as antigen.	giemsa-stained, peripheral blood smears of 67 dogs, showing clinical signs typical of babesiosis or reminiscent of concurrent babesiosis and ehrlichiosis, were examined for the presence of babesia canis and ehrlichia canis. since cowdria ruminantium cross-reacts with ehrlichia, the sera of these dogs were also subjected to the indirect fluorescent antibody (ifa) test in which c. ruminantium was used as antigen. fifty-five per cent of these dogs had mixed infections of b. canis and e. canis, as j ...	1990	2234860
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
halophilic proteins and the influence of solvent on protein stabilization.	competition between protein-solvent and protein-protein interactions is arguably the most important contributing factor to polypeptide folding in general. a study of halophilic proteins, correlating their stability and solution structures in different conditions, focuses on the effects of a high salt solvent. a mechanism is proposed to explain how these proteins have adapted to such an extreme environment.	1990	2238041
recombinant dna probe detecting eperythrozoon suis in swine blood.	a genomic library to eperythrozoon suis dna was constructed in lambda gt11, and from this library, e suis clone ksu-2 was identified as a potential diagnostic probe. in hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the ksu-2 probe hybridized strongly with purified e suis organisms and blood samples from splenectomized swine that were parasitized with e suis. however, the probe under stringent conditions did not give radiographic indi ...	1990	2240802
rolling chair sign.		2007	2241609
acute upper respiratory tract viral illness and influenza immunization in homes for the elderly.	occupants of 482 long-stay and 33 short-stay beds in 11 leicester city council homes for the elderly were studied during a 30-week period from september 1988 to march 1989 to determine the incidence, aetiology, morbidity, and mortality of acute upper respiratory tract viral infections and the use of influenza vaccine. influenza immunization rates by home ranged from 15.4 to 90% (mean 45%). there were no differences in the distribution of medical conditions by home. the highest immunization rates ...	1990	2249724
stimulatory effects of prostaglandin d2 analogues on adenylate cyclase in rabbit iris-ciliary body membrane fractions.	effects of prostaglandin (pg) d2 analogues on the adenylate cyclase activity in membrane fractions of the iris-ciliary body complex were studied. pgd2 dose-dependently activated the adenylate cyclase with a maximal activity increase of about 60%. the concentration required to cause a half-maximal stimulation (ec50) was about 5 x 10(-7) m. the stimulatory effect of pgd2 was totally dependent on gtp with ec50 for gtp at about 10(-7) m. the rank order of potency of pgd2 analogues for stimulating th ...	1990	2249731
self-screening of blood pressure and sodium in a 24-hour urine sample as part of a school health programme.	a study was conducted to determine the usefulness of self-screening of blood pressure in families as part of a school health care programme, and to study the relationship between bp and sodium excretion in school children. in 750 families with 10-13 year old children, the mothers were instructed to take bp measurements on themselves, their child and the father whilst at home. a comparison of the children's bp measurements at home and at school with those of the parents showed the home measuremen ...	2008	2258868
biochemical analysis of related, independently arising histocompatibility mutants: bm17 and kb-98 enlarge the "bg series" of h-2kb mutants.	the "bg" series of mhc mutations is the most prevalent type of mutations of kb in c57bl/6 mice screened by reciprocal tail skin grafting. the basis for identification of this series of mutations is the incompatibility of grafts between the parental b6 and the mutant. this series takes the longest to reciprocally reject the skin grafts. the series can be subdivided into "bg 1" and "bg 2" groups based on kb-restricted recognition of virus-infected mutant target cells. the biochemical basis for the ...	2008	2268259
biochemical analysis of related, independently arising histocompatibility mutants: bm17 and kb-98 enlarge the "bg series" of h-2kb mutants.	the "bg" series of mhc mutations is the most prevalent type of mutations of kb in c57bl/6 mice screened by reciprocal tail skin grafting. the basis for identification of this series of mutations is the incompatibility of grafts between the parental b6 and the mutant. this series takes the longest to reciprocally reject the skin grafts. the series can be subdivided into "bg 1" and "bg 2" groups based on kb-restricted recognition of virus-infected mutant target cells. the biochemical basis for the ...	2008	2268259
[characteristics of the functional state of the nervous system of middle-aged persons during anti-orthostatic hypokinesia].	the functional state of the nervous system and its autonomic compartment was investigated in 15 men, aged 40-52 years, during their 30-day exposure to head-down tilt (-8 degrees). group 1 consisted of 9 subjects with early manifestations of cerebral atherosclerosis and group 2 included 6 subjects with cerebral atherosclerosis and arterial hypertension. the control group incorporated 9 healthy volunteers, aged 25-39 years. during adaptation to head-down tilt, middle-aged and young subjects showed ...	2008	2292866
canine ehrlichiosis in connecticut.	the first case of canine ehrlichiosis in connecticut is reported. a female brittany spaniel from milford presented with lethargy, anorexia, fever, petechiae, splenomegaly, thrombocytopenia, anemia, elevated serum alkaline phosphatase, lymphopenia, and hypoalbuminemia. serologic analysis revealed antibodies to ehrlichia canis (titer, 1:2,560). this documents a more northern geographic distribution in the united states for this infectious agent than had previously been suspected.	1990	2312682
epidemiologic and clinical characteristics of persons with serologic evidence of e. canis infection.	the results of a serosurvey of oklahomans for the presence of antibody to ehrlichia canis is reported. paired serum specimens, from patients lacking the serologic criteria for diagnosis of rocky mountain spotted fever (rmsf), were tested. a four-fold increase in e. canis-ifa antibody was found in 16/144 (11 percent) of these paired serum samples. patients with serologic evidence of e. canis infection had a mean age of 34 years, 69 percent were male, and 63 percent lived in a town less than 10,00 ...	1990	2316766
plasma and urinary levels of carnitine in different experimental models of hyperammonemia and the effect of sodium benzoate treatment.	the effect of hyperammonemia on plasma and urinary levels of carnitine was studied in different groups of +/y (normal) and spf/y (chronically hyperammonemic) mice. experimental models of acute and subacute hyperammonemia were prepared in +/y and spf/y mice by the use of ammonium acetate ip injections and arginine-free diets, respectively. in acute hyperammonemia, the plasma levels of both free and acylcarnitines increased significantly whereas acyl/free carnitine ratio was decreased, indicating ...	1990	2346672
transient palsy of hip abductors after a fall on the buttocks.	a fall on the buttocks caused monolateral transient palsy of the hip abductors in two patients. palsy could be ascribed to acute entrapment of the superior gluteal nerve between the piriformis muscle and the incisura ischiadica major.	2008	2346715
the insulin-like growth factor i (igf-i) gene is expressed in chick embryos during early organogenesis.	a definition of the role of igf-i in differentiation and development requires a detailed understanding of its expression and tissue-specific regulation in embryogenesis. standard techniques for analysis of igf-i gene expression are not sufficiently sensitive for studies in early embryos. we have used the highly sensitive polymerase chain reaction (pcr) to study igf-i gene expression in whole chick embryos from the late blastula stage (e0 = laying) through the end of organogenesis (day 8), and in ...	1990	2387267
is androgen-dependent aromatase activity sexually differentiated in the rat and dove preoptic area?	aromatase activity is higher in the male than in the female anterior hypothalamic-preoptic area (poa) in both the avian and the rodent adult brain. this sex difference is abolished after castration of the male and restored by androgen treatment. gonadectomy has no effect on poa aromatase in the female. the aim of this study was to find out whether sex dimorphism in adult poa aromatase is only due to a sex difference in circulating gonadal hormones or dependent upon sexual differentiation of the ...	1990	2394992
"synaptic" ribbons in the pineal gland of the horse.	previous studies on the ultrastructure of the horse pineal gland did not report the presence of "synaptic" ribbons, functionally enigmatic pinealocyte organelles regularly occurring in other mammalian species. the aim of the present study was to reinvestigate the horse pinealocyte in this respect. careful investigations here reported allowed detection of "synaptic" ribbons in the pinealocytes of all the examined animals, although in a relatively low number (5.4 +/- 2.0 per 20,000 microns 2 of pi ...	1990	2395075
use of the megasoft bandage lens for treatment of complications after trabeculectomy.	shallow anterior chambers and leaking filtration blebs are possible complications after trabeculectomy that can be treated with therapeutic contact lenses. in most cases, however, treatment fails because these lenses are not large enough to cover the filtering bleb. we evaluated the use of a newly developed large diameter (20.5 mm) therapeutic soft contact lens. five patients with shallow anterior chambers and ten patients with leaking filtering blebs after trabeculectomy were fitted with this n ...	2008	2396651
[interaction of local anesthetics with secretory cells at the membrane level].		1985	2408715
the perivascular cell populations in human skin after topical application of leukotriene b4.	fifteen timed biopsies of human skin were performed after application of 100 nanograms of leukotriene b4 in a finnchamber for six hours. the number of inflammatory cells were counted per high power perivascular field and compared to three control biopsies. at 24 hours a peak of neutrophiles was observed and subsequently lymphocytes predominated. eosinophils were never prominent. this pattern of successive cell populations has been described in inflammatory and whealing skin disease.	1986	2430407
[changes in protein and rna synthesis following acute hindbrain ischemia].	protein and rna synthesis of the brain is affected by focal transient ischemia. protein synthesis is depressed by the depletion of energy metabolism during ischemia, and its recovery following recirculation is slower than restoration of energy metabolism. on the other hand, rna synthesis is more tolerable to ischemia than protein synthesis. present study has designed to evaluate changes of protein and rna synthesis of the brain after ischemia. we used a hindbrain ischemia model of gerbils, and q ...	2008	2446643
a serological survey of ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi in dogs in oklahoma.	serum samples from 259 dogs were tested for antibodies to ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi using the indirect fluorescent antibody test. the sera were obtained from submissions to the oklahoma animal disease diagnostic laboratory during a 14-month period from june 1986 through july 1987. the rate for positive antibody titers to e. canis was 53%, to e. equi was 33%, to r. rickettsii was 38%, and to b. burgdorferi was 18%. higher percentages of sera ...	1989	2518693
mechanisms and kinetics for platelet and neutrophil localization in immune complex nephritis.	we have previously reported that both neutrophils (pmns) and platelets mediate proteinuria in a model of subendothelial immune complex (ic) nephritis (gn) in the rat. in order to understand the interaction of pmns and platelets in this model, we quantitated the uptake of 111in-labelled platelets in glomeruli and correlated this with the number of pmns observed histologically at 10 and 30 minutes, 1, 4 and 24 hours following induction of gn. platelet accumulation was biphasic with a major peak at ...	1989	2533296
transport of nutrients into the renal brush border membrane vesicles as marker in evaluating the role of antipili antibodies in modulation of ascending pyelonephritis in rats.	the uptake of d-glucose, l-aspartate, l-lysine and l-proline was investigated in renal brush border membrane (bbm) vesicles prepared from control, infected or passively-immunized-infected rats. except l-aspartate, a progressive decrease in the uptake of these nutrients in both infected and immunized-infected groups during the course of infection was observed, but the changes were less apparent in immunized-infected rats than in non-immunized ones. the uptake of l-aspartate was increased in vesic ...	1989	2566554
diagnosis of peripheral androgen insensitivity in a male infant excretion analysis.	the hypothesis of peripheral androgen insensitivity (ais) was examined in a boy with congenital growth hormone deficiency associated with micropenis and cryptorchidism by steroid excretion analyses compared with dihydrotestosterone (dht) receptor analyses of foreskin biopsy homogenate. urinary t metabolite 3 alpha,17 beta-dihydroxy-5 beta-androstane (3 beta-diol) was below the limit of detection (capillary gas chromatography) at age 1 year, but on several occasions (n = 7) normal basal values we ...	2008	2568823
new cd4(+) cell line susceptible to infection by hiv-1.	infection of a newly described human t lymphoid cell line, cem-cl10, with three different variants of hiv-1 resulted in cytopathic effects followed by cell lysis. following primary lytic infection, proviral dna could not be detected by southern blot analysis in the outgrowth of the surviving cem-cl 10 cells 60 days after initial exposure to hiv-1. these surviving cells could be further grown as a separate line, derived from cem-cl10, and were found to be resistant to subsequent infection by hiv- ...	1989	2570823
different gamma-glutamyl transpeptidase mrnas are expressed in human liver and kidney.	in human, the two subunits of gamma-glutamyl transpeptidase (ggt) arise from a common precursor encoded by a multigene family. until now, a single specific coding sequence for this precursor (type i) has been identified in human placenta and liver. in the present study, we have isolated from a human kidney cdna library, a ggt specific clone (0.8 kb). the sequence of which (type ii) i) covers the carboxy terminal part of the ggt precursor, ii) exhibits 22 point mutations and a 30 bp deletion as c ...	1989	2573352
copper(i)-bleomycin: structurally unique complex that mediates oxidative dna strand scission.	copper(i)-bleomycin [cu(i) x blm] was characterized in detail by 13c and 1h nmr. unequivocal chemical shift assignments for cu(i) x blm and cu(i) x blm x co were made by two-dimensional 1h-13c correlated spectroscopy and by utilizing the observation that cu(i) x blm was in rapid equilibrium with cu(i) and metal-free bleomycin, such that individual resonances in the spectra of blm and cu(i) x blm could be correlated. the binding of cu(i) by bleomycin involves the beta-aminoalaninamide and pyrimid ...	2008	2581602
focus of human parasitism by the brown dog tick, rhipicephalus sanguineus (acari: ixodidae).	throughout most of its range, the brown dog tick, rhipicephalus sanguineus (latreille), prefers dogs as a host, but human bites occasionally occur in the mediterranean region and central america; historically, this species has rarely bitten humans in the united states. a focus of 15 human bite cases by this tick is reported from four air force bases located within 200 miles of each other in north central texas and southwestern oklahoma. the sudden appearance of numerous documented bite cases ind ...	1989	2585458
ehrlichiosis in a dog with seizures and nonregenerative anemia.	ehrlichia canis infection was diagnosed in a dog with a history of seizures and nonregenerative anemia. serologic titer to e canis was greater than 1:100. evaluation of csf revealed a high cell count, high protein concentration, and a positive pandy test result. several mononuclear leukocytes in the csf contained e canis morulae. central nervous system lesions are commonly found on postmortem examination of animals with ehrlichiosis, although clinical reports of neurologic signs attributable to ...	1989	2599963
[drug-induced cardiovascular complications].	many drugs, including those prescribed in cardiology, can induce adverse cardiovascular side effects. most of the anti-arrhythmia drugs have a negative inotropic action and proarrhythmic effects. estroprogestative contraceptive drugs favor thromboembolitic events and increase the incidence of hypertension. due to the risk of coronary insufficiency, substitutive hormonotherapy in hypothyroidism must be introduced gradually. tricyclic anti-depressive drugs and neuroleptics can induce orthostatic h ...	2008	2610453
[pain in the trochanteric region caused by tunnel compression of the lateral cutaneous perforating branch of the ilio-hypogastric nerve. indications for neurolysis].	after consideration of anatomical and clinical studies, the authors describe a new tunnel syndrome involving the lateral cutaneous branch of the iliohypogastric nerve as it emerges above the iliac crest. irritation of the strangulated nerve produces pain over the lateral aspect of the hip. in 7 cases where local infiltration failed, neurolysis was carried out and produced excellent results in 5 patients, thus confirming the pathophysiology of this syndrome.	2008	2612275
[pain in the trochanteric region caused by tunnel compression of the lateral cutaneous perforating branch of the ilio-hypogastric nerve. indications for neurolysis].	after consideration of anatomical and clinical studies, the authors describe a new tunnel syndrome involving the lateral cutaneous branch of the iliohypogastric nerve as it emerges above the iliac crest. irritation of the strangulated nerve produces pain over the lateral aspect of the hip. in 7 cases where local infiltration failed, neurolysis was carried out and produced excellent results in 5 patients, thus confirming the pathophysiology of this syndrome.	2008	2612275
[mucous nasosinus melanoma. apropos of a case].	the mucous melanoma is a rare affection which has a very poor prognosis by its difficult diagnosis and unsatisfactory treatment. we have a patient with mucous melanoma nasal, which has been studied (clinic evolution, diagnosis and treatment) in our hospital.	2001	2631901
ehrlichia canis: a tick-borne rickettsial-like infection in humans living in the southeastern united states.	during the past two years, sporadic cases of a rickettsial-like illness were reported in humans living in the southeastern united states. the illness was serologically similar to ehrlichia canis infections in dogs. it resembled spotless rocky mountain spotted fever but was differentiated from this infection serologically with acute and convalescent sera showing increasing titers to ehrlichia canis. e. canis infection should be suspected in patients with fever, headache, malaise, myalgia, gastroi ...	1989	2643878
successful implantation of penile prostheses in organ transplant patients.	we present our experience with implantation of penile prostheses in 6 organ transplant recipients, including 2 patients after renal transplantation and 4 patients after cardiac transplantation. we have seen no problems related to prosthesis surgery in this patient population, and in particular have experienced no infectious complications. all patients received strict perioperative antibiotic and steroid coverage. we conclude that erectile impotence in the male organ transplant patient can be suc ...	2009	2648658
the roles of stem cell self-renewal and autocrine growth factor production in the biology of myeloid leukemia.		2009	2650863
foreign nations, international organizations, and their impact on health conditions in nicaragua since 1979.	in july 1979, a coalition of social forces in nicaragua, under the leadership of the sandinistas, toppled the discredited 43-year somoza dictatorship. in addition to revolutionary nicaragua's own substantial efforts, since 1979 international forces and developments have had profound impacts on the nation's ambitious social programs. this article investigates the impact of foreign nations and international organizations on nicaragua's health conditions since 1979. given or pledged assistance, for ...	1989	2666319
use of phenytoin in healing of war and non-war wounds. a pilot study of 25 cases.	nineteen patients with war-related missile wounds and six with refractory civilian ulcers were treated with topical phenytoin sodium powder daily for up to 4 weeks. the mean healing time was 2 weeks for missile wounds, compared to historical controls requiring 6-8 weeks. healing time was 4 weeks for civilian ulcers that had been unresponsive to any treatment over the previous 5 months. twenty-two patients had complete healing, three required skin grafts. wider use of this safe, inexpensive, read ...	2009	2666326
ehrlichiosis--"spotless spotted fever".	ehrlichia canis, a canine rickettsia, produces an acute infection in humans characterized by thrombocytopenia, leukopenia, and anemia. patients should be closely questioned and examined for tick bites. rash is not prominent, leading to the interesting if somewhat inaccurate label of "spotless spotted fever." the diagnosis is made by clinical presentation, absence of positive lyme disease or rocky mounted spotted fever (rmsf) serology, and positive e. canis titers. tetracyclines are preferred tre ...	1989	2668039
[tumor metastasis and growth factors].	massive investigations are being carried out on growth factors. growth factors are an important determinant for the formation of successive tumor metastasis at target organs. we review the findings, including our data, of growth factors in relation to tumor metastasis.	1989	2679395
[prevention of stomatitis induced by anti-cancer drugs].	a randomized control trial study was carried out to evaluate the effect of allopurinol mouth wash on stomatitis induced by chemotherapy in gynecologic patients. chemotherapeutics used consisted of 5-fu+cddp (pf) given to 10 patients and vincristine and actinomycin-d+cyclophosphamide (vac) given to 5. allopurinol mouth wash was prepared for patients to rinse their mouth with the solution 4-5 times daily before and after treatment with anti-cancer drugs. the japan society for cancer therapy's crit ...	2009	2679397
induction of acantholysis in organ explant culture by penicillamine and captopril.	pemphigus is an autoimmune disease proved to be mediated by igg autoantibodies. skin lesions clinically and histologically identical to pemphigus may occur in patients receiving penicillamine and captopril, but some of these patients lack circulating or tissue-bound autoantibodies. therefore, we examined the ability of these drugs to produce acantholysis directly in organ explant culture. human skin explants were prepared from split-thickness graft skin from adults and from neonatal foreskins. e ...	1989	2679399
growth inhibitors: molecular diversity and roles in cell proliferation.		2009	2681129
placental transport of calcium and phosphorus in pigs.	the role of vitamin d in regulating placental transport of calcium and phosphate in pigs was studied using the "hannover pig strain" which suffers from pseudo-vitamin d-deficiency rickets, type i. sows and fetuses of normal phenotype (heterozygotes) and of homozygote phenotype which suffered from clinical symptoms of rickets were used. the homozygote animals are devoid of renal 25-cholecalciferol-1-hydroxylase. in the rachitic sows which normally depend on treatment with pharmacological doses of ...	1989	2681666