Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| chemical permeabilization of cells for intracellular product release. | 2006 | 1366880 | |
| separations for biotechnology. | 2006 | 1366893 | |
| a high containment polymodal pilot-plant fermenter--design concepts. | a 225 dm3 pilot-plant bioreactor system has been designed and constructed that is suitable for biohazardous fermentations. the design enables operation at containment levels above the requirements of good industrial large-scale practice (gilsp) without secondary containment of the whole plant. the main biosafety features of the systems include the use of steam barriers on o-ring seals, supply lines and stirrer seals, multiple o-ring seals, piping of condensate lines and pressure relief systems t ... | 2006 | 1367227 |
| [the ototoxicity of the cytostatic drug carboplatin in patients with head-neck tumors]. | the side effects of the anti-cancer drug carboplatin on the cochlea in 65 patients with head and neck cancer were examined in the present study. a possible dependence of ototoxicity on the patient's age, the effect of pre-existing audiometric changes and the carboplatin dose were investigated. a therapy-induced sensory hearing loss was found in 32% of the patients. the average hearing loss was 15 db and primarily involved the frequency ranges from 4 to 8 khz. the degree of the hearing defects in ... | 1995 | 1388466 |
| health checks on patients 75 years and over in nottinghamshire after the new gp contract. | to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners. | 2006 | 1393076 |
| asymmetrical brain modulation of the immune response. | it is now well known that the central nervous system can regulate the immune system. interestingly the two sides of the brain have been demonstrated to be differently involved in the modulation of immune responses. in rodents, lesions of right or left neocortex induced opposite effects on various immune parameters including mitogen-induced lymphoproliferation, interleukin-2 production, macrophage activation or natural killer cell activity. furthermore in humans, left-handedness has been reported ... | 2006 | 1393189 |
| sheen formation and growth response of groundwater bacteria to reduced oxygen concentrations during incubation of m-endo medium. | in vitro pure-culture studies were conducted to assess growth and sheen formation of groundwater bacteria on m-endo medium incubated under reduced oxygen concentrations (0, 4, 8, 12, and 16%). coliform and noncoliform bacteria were isolated from 17 untreated, rural groundwater supplies on m-endo medium. all 16 coliform isolates tested were capable of sheen formation at oxygen concentrations of 4% or greater, yet some of these same isolates (enterobacter aerogenes, enterobacter cloacae, and hafni ... | 1992 | 1393829 |
| the structure of a glycerol teichoic acid-like o-specific polysaccharide of hafnia alvei 1205. | the o-specific polysaccharide of hafnia alvei 1205 contained d-glucose, d-galactose, 2-acetamido-2-deoxy-d-glucose, 4-acetamido-4,6-dideoxy-d-glucose (qui4nac), glycerol, phosphate, and o-acetyl groups. on the basis of 1d and 2d shift-correlated homonuclear and 13c-1h heteronuclear nmr spectroscopy, methylation analysis, smith degradation, and dephosphorylation with hydrofluoric acid, it was concluded that the o-antigen was a partially o-acetylated teichoic acid-like polysaccharide having the fo ... | 1992 | 1394318 |
| the structure of the o-specific polysaccharide from hafnia alvei strain 38 lipopolysaccharide. | the o-specific polysaccharide of the lipopolysaccharide from h. alvei strain 38 has been established by nmr spectroscopy (13c and 1h) and methylation analysis to have the repeating unit-->4)-beta-d-manpnac-(1-->4)-alpha-d-glcpnac(1-->. | 1992 | 1394327 |
| alw26i, eco31i and esp3i--type iis methyltransferases modifying cytosine and adenine in complementary strands of the target dna. | the specificity of three dna methyltransferases m.alw26i, m.eco31i and m.esp3i, isolated from acinetobacter iwoffi rfl26, escherichia coli rfl31 and hafnia alvei rfl3+, respectively, was determined. all the enzymes methylate both strands of asymmetric recognition sites yielding m5c in the top-strand and m6a in the bottom-strand, as below: 5'-gtm5ctc 5'-ggtm5ctc 5'-cgtm5ctc 3'-cm6agag 3'-ccm6agag 3'-gcm6agag (m.alw26i) (m.eco31i) (m.esp3i) they are the first members of type iis methyltransferases ... | 1992 | 1408816 |
| sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic escherichia coli and hafnia alvei. | seven strains of hafnia alvei isolated from diarrhoeal stools of children resembled enteropathogenic escherichia coli (epec) in that they produced attaching-effacing (ae) lesions in rabbit ileal loops and fluorescent actin staining in infected hep-2 cells. in addition, a dna probe from a chromosomal gene required by epec to produce ae lesions, hybridised to chromosomal dna from all seven h. alvei strains. these findings indicate that there is a sharing of virulence-associated properties at the p ... | 1992 | 1433251 |
| analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry. | fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ... | 1992 | 1447059 |
| diaphragmatic movement using ultrasound during spontaneous and mechanical ventilation: effect of tidal volume. | using ultrasound (us) the effect of various tidal volumes on the movement of ventral, dome and dorsal parts of the right hemidiaphragm was studied, both during spontaneous and mechanical ventilation. six healthy non-medicated volunteers who were in the supine position breathed spontaneously shallowly (tidal volume (vt) being 400 ml) (sb), and deeply (vt 1000 ml) (sb-deep). in addition, they were mechanically ventilated with intermittent positive pressure ventilation at three different vt's: 500 ... | 2006 | 1449048 |
| non-invasive thermometry using magnetic resonance diffusion imaging: potential for application in hyperthermic oncology. | the proposition to use non-invasive thermometry based on magnetic resonance diffusion imaging for applications in therapeutic hyperthermia is examined. the measurement of proton motion predominantly associated with the self-diffusion of water can be characterized by a boltzmann temperature dependence (i.e. e-ea/kt). the activation energy (ea) is on the order of 0.2 ev and, for a restricted range (approximately 30 degrees) at a base temperature of approximately 300 k, the relationship between the ... | 2006 | 1479207 |
| model-based interpretation of the ecg: a methodology for temporal and spatial reasoning. | a new software architecture for automatic interpretation of the electrocardiogram is presented. using the hypothesize-and-test paradigm, a semi-quantitative physiological model and production rule-based knowledge are combined to reason about time- and space-varying characteristics of complex heart rhythms. a prototype system implementing the methodology accepts a semi-quantitative description of the onset and morphology of the p waves and qrs complexes that are observed in the body-surface elect ... | 2006 | 1482855 |
| tiny echogenic foci in the liver and kidney in patients with aids: not always due to disseminated pneumocystis carinii. | 1992 | 1489361 | |
| in vitro activity and beta-lactamase stability of ljc 10,627. | the in vitro activity of ljc 10,627, a new carbapenem, was compared with those of imipenem, cefotaxime, ceftazidime, and gentamicin. ljc 10,627 inhibited 90% of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter aerogenes, enterobacter agglomerans, enterobacter cloacae, hafnia alvei, citrobacter freundii, citrobacter diversus, proteus mirabilis, morganella morganii, proteus rettgeri, serratia marcescens, pseudomonas cepacia, salmonellae, shigellae, aeromonas, and yersiniae ... | 1992 | 1510436 |
| disruption of the gene encoding the secreted acid protease (acp) in the yeast candida tropicalis. | the gene for the secreted acid protease (acp), a potential virulence factor of candida species, was inactivated in candida tropicalis by gene disruption. the disruption was performed by cotransformation of an ade2 c. tropicalis mutant with a linear dna fragment carrying a deletion in acp, and the replicative vector pmk16 which carries a selectable ade2 gene marker. few of the transformants exhibited lower protease secretion levels and were shown to have one deleted and one unaffected acp copy, s ... | 1992 | 1526448 |
| nucleotide sequence of the escherichia coli cad operon: a system for neutralization of low extracellular ph. | lysine decarboxylase of escherichia coli has been the subject of enzymological studies, and the gene encoding lysine decarboxylase (cada) and a regulatory gene (cadr) have been mapped. this enzyme is induced at low ph in the presence of lysine and achieves maximal level under anaerobic conditions. the induction of lysine decarboxylase increases the ph of the extracellular medium and provides a distinctive marker in tests of clinical strains. we report the sequence of the cad operon encoding lysi ... | 1992 | 1556085 |
| ru 29 246, the active compound of the cephalosporin-prodrug-ester hr 916. i. antibacterial activity in vitro. | the aminothiazolyl-cephalosporin ru 29 246 is the active metabolite of the prodrug-pivaloyl-oxyethyl-ester hr 916. ru 29 246 in vitro activity includes a wide range of clinically relevant bacterial pathogens. against methicillin-sensitive staphylococci ru 29 246 (mic90 of 0.25 approximately 2 micrograms/ml) was clearly more active than cefaclor, cefuroxime, cefpodoxime, cefixime and ceftibuten, but slightly less active than cefdinir. ru 29 246 inhibited hemolytic streptococci of the serogroups a ... | 1992 | 1592683 |
| daily rhythmicity of the rat acoustic startle response. | we have measured the acoustic startle response (asr) amplitude and latency in rats housed in a 12:12 light:dark (ld) cycle. the response amplitudes to eliciting stimuli (es) of 110 db or 120 db (white noise) were significantly higher (nearly two-fold) during d than during l. similar, but nonsignificant, trends were also observed at es intensities of 90 db or 100 db. while some significant ld asr latency differences were observed, we cannot ascribe them to the photoperiodic phase at this time. th ... | 1992 | 1594689 |
| [colitis and hafnia alvei]. | 1992 | 1602859 | |
| acute bacterial gastroenteritis caused by hafnia alvei. | 1992 | 1623093 | |
| chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi. | linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ... | 1991 | 1657086 |
| in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin). | the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ... | 1991 | 1664418 |
| [clinical significance of des-gamma-carboxy prothrombin assay as a tumor marker]. | 2006 | 1693989 | |
| immunological tolerance to many self epitopes may be unnecessary. | the demonstration of the existence of antigenic mimicry suggests that immunological tolerance to self antigens may be an insufficient basis for distinguishing self from non-self. however, some data suggest that even in the absence of tolerance most self epitopes would not immunize the host and that tolerance to all self epitopes may, therefore, not be necessary for the prevention of autoimmune disease. | 2006 | 1698308 |
| monoclonal antibodies to quebec strain (q17) of bovine rotavirus. | fourteen monoclonal antibodies (mabs) against "quebec" strain (q17) of bovine rotavirus were isolated and characterized. four were specific for viral protein vp7 and ten were specific for viral protein vp6. five different isotypes were represented by this group of antibodies. all of the anti-vp6 and none of the anti-vp7 antibodies were sensitive to the effects of periodate on their antigen. the antibodies could be separated into three groups based on their relative resistance to the dissociation ... | 1990 | 1698323 |
| effects of interleukin-2 (il-2) on human plasma lipid, lipoprotein, and c-reactive protein. | six patients with confirmed malignant disease received four consecutive weekly cycles of human recombinant interleukin-2 (il-2) 4 days/week, continuous iv. infusion, 3 x 10(6) u/m2/day. plasma cholesterol decreased a mean of 7% within 24 hours after il-2 infusion and decreased by 33% within 4 days. plasma cholesterol was significantly lower than baseline concentration by day 21 (-21%), and day 25 (-41%) was significantly lower than day 21. decreased plasma cholesterol was the result of decreased ... | 1990 | 1698421 |
| the structure of the biological repeating unit of the o-antigen of hafnia alvei o39. | on mild acid-catalysed degradation of the lipopolysaccharide from hafnia alvei o39 followed by gel filtration of sephadex g-50, the o-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 o-antigen repeating-units. on the basis of sugar and methylation analyses, 13c-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13c-n.m.r. analysis of the smith-degraded polysaccharide, it was conclude ... | 1990 | 1703456 |
| [the identification of lactose-negative enterobacteria]. | study of the morphologic and enzymatic characteristics of 10 strains of lactose-negative enterobacteria, sent to the l. a. tarasevich state institute for control and standardization as b. paracoli in order to determine their taxonomic status in accordance with the 1974 and 1984 bergey classifications has referred 9 of these strains to hafnia alvei. | 1990 | 1705611 |
| [diagnosis and therapy of viral hepatitis]. | 1991 | 1713243 | |
| o-specific polysaccharide of hafnia alvei lipopolysaccharide isolated from strain 1211. structural study using chemical methods, gas-liquid chromatography/mass spectrometry and nmr spectroscopy. | the hafnia alvei strain 1211 o-specific polysaccharide is composed of 3-amino-n-(d-3'-hydroxybutyryl)-3,6-dideoxy-d-galactose, n-acetyl-d-galactosamine, n-acetyl-d-glucosamine and d-glucose (1:1:2:2). on the basis of sugar and methylation analyses, smith degradation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, the polysaccharide was shown to be an o-acetylated polymer of the repeating hexasaccharide unit, ----2d(4-oac)fucp3nacyl beta 1----6dglcpnac alpha 1---- (dglcp beta 1----3)4 ... | 1991 | 1716201 |
| structural studies on hafnia alvei 114-60 o-antigen. | the structure of hafnia alvei 114-60 o-antigen has been established using sugar and methylation analyses as well as 1h-nmr spectroscopy. the results obtained proved that the repeating unit of hafnia alvei 114-60 o-antigen is identical to that of hafnia alvei atcc 13337 standard strain and has the following structure: [formula: see text] | 1990 | 1718234 |
| [hafnia alvei septicemia with shock and dic in an adult with postoperative lung cancer]. | in japan, we experienced the first case of hafnia alvei septicemia with shock and disseminated intravascular coagulation (dic) in an adult with postoperative lung cancer. a 63 year-old male, who had been followed up in our department since 1987, was admitted to our hospital with the complaints of fever, hemoptysis and dyspnea on june 25, 1989. after admission, he was treated with sulbactam/cefoperazone 4 g/day intravenously for suspicion of respiratory-tract infection. after antibiotic administr ... | 1991 | 1761901 |
| changes in the glomerular pore size selectivity in patients with type ii diabetes mellitus. | we measured the urinary excretion rate and clearance of three plasma proteins, albumin, transferrin, and igg4, each of which has a similar isoelectric point, but a different molecular weight. this study consisted of 86 patients with type ii diabetes mellitus and 15 healthy subjects. in the patients, the degree of the urinary excretion rate and clearance of both transferrin (ter and ctrans) and igg4 igg4 er and clgg4) closely correlated with that of the urinary excretion rate of albumin (aer). al ... | 2003 | 1770023 |
| identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test. | we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ... | 1991 | 1774276 |
| effect of blood transfusion on plasma selenium, glutathione peroxidase and manganese levels in very low birth weight infants. | very low birth weight infants often receive multiple blood transfusions. we measured the plasma levels of the trace elements selenium, manganese, and glutathione peroxidase in 20 very low birth weight infants prior to blood transfusion and then at 24, 48 and 72 h after transfusion. there was no detectable change in mean selenium or glutathione peroxidase concentrations after transfusion, but the mean (sd) plasma manganese increased from 3.8 (1.5) to 6.0 (2.3) micrograms/l at 72 h. | 2006 | 1797115 |
| effect of blood transfusion on plasma selenium, glutathione peroxidase and manganese levels in very low birth weight infants. | very low birth weight infants often receive multiple blood transfusions. we measured the plasma levels of the trace elements selenium, manganese, and glutathione peroxidase in 20 very low birth weight infants prior to blood transfusion and then at 24, 48 and 72 h after transfusion. there was no detectable change in mean selenium or glutathione peroxidase concentrations after transfusion, but the mean (sd) plasma manganese increased from 3.8 (1.5) to 6.0 (2.3) micrograms/l at 72 h. | 2006 | 1797115 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| the effect of exposure to impulse x rays on normal and epileptic activity in rabbit brain. | exposure to x rays (20 impulses of 4 hz frequency, total dose 0.6-1.1 mgy) increased the epileptic activity of a focal area, which was produced in the visual cortex of rabbit brain by freezing with liquid nitrogen, and by stimulating with flashes of light at frequencies of 5-6 hz. the number of seizure complexes during photostimulation for 5 s increased by 80% compared with the initial level, and this effect continued for 15 min. in control animals (with no epileptic foci), a decrease was observ ... | 1991 | 1797142 |
| serological characterization of lipopolysaccharides of hafnia alvei 1, 17, 31, 32, 38 and 39. | lipopolysaccharides of hafnia alvei strains 1, 17, 31, 32, 38 and 39 indicate strong cross-reactivity in passive hemagglutination. all lipopolysaccharides were examined by rocket immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting. | 1991 | 1804049 |
| [clinical significance of the isolation of hafnia alvei from the feces of children with acute gastroenteritis]. | 1991 | 1822161 | |
| cost, controversy, crisis: low back pain and the health of the public. | 2006 | 1828670 | |
| astrocytes: a possible primary site for colchicine-mediated neurotoxicity in the rat striatum. | ultrastructural changes in rat astrocytes including endfoot swelling and rupture of plasma membranes were seen as early as 1 h after injection of 6.0 micrograms colchicine (co) into the striatum. significant change in neuronal morphology was not seen by 24 h. astrocytes may therefore be primary targets for co-mediated damage, which then may have secondary neurotoxic consequences. | 1991 | 1828783 |
| sex hormone binding globulin, cortisol binding globulin, thyroxine binding globulin, ceruloplasmin: changes in treatment with two oral contraceptives low in oestrogen. | it is generally assumed that the oral contraceptives cause the carrier proteins to change. notoriously this effect is used to evaluate indirectly their estrogenicity/gestagenicity ratio. in order to assess the residual intrinsic androgenic activity of two new 19-nor-derivative components, desogestrel (dg) 150 micrograms and gestodene (gd) 75 micrograms, both in association with ethinylestradiol (ee) 30 micrograms, sex hormone binding globulin, thyroxine binding globulin, ceruloplasmin and free a ... | 2006 | 1829029 |
| sex hormone binding globulin, cortisol binding globulin, thyroxine binding globulin, ceruloplasmin: changes in treatment with two oral contraceptives low in oestrogen. | it is generally assumed that the oral contraceptives cause the carrier proteins to change. notoriously this effect is used to evaluate indirectly their estrogenicity/gestagenicity ratio. in order to assess the residual intrinsic androgenic activity of two new 19-nor-derivative components, desogestrel (dg) 150 micrograms and gestodene (gd) 75 micrograms, both in association with ethinylestradiol (ee) 30 micrograms, sex hormone binding globulin, thyroxine binding globulin, ceruloplasmin and free a ... | 2006 | 1829029 |
| a review of the safety profile of teicoplanin. | safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ... | 2006 | 1829079 |
| a review of the safety profile of teicoplanin. | safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ... | 2006 | 1829079 |
| consultation-liaison psychiatry. does it help to get organized? the european consultation-liaison workgroup. | since 1987 consultation-liaison (c-l) psychiatrists in europe have decided to develop a closer collaboration to stimulate the development of the c-l field. a first result of this collaboration is a companion paper on the history and current state of c-l psychiatry in europe. this paper describes the arguments for the development of the european consultation-liaison workgroup for general hospital psychiatry and psychosomatics (eclw), and the results of the first 3 years of collaboration. | 2007 | 1855658 |
| mitochondrial damage by active oxygen species in vitro. | under in vitro conditions involving formation of active oxygen species, rat liver mitochondria were found to undergo swelling, peroxidative decomposition of lipids, and distinct disorganization of ultrastructure. supplementation with free radical scavengers such as superoxide dismutase (sod), methionine, histidine, and tryptophan accorded considerable protection to the organelle. a possible correlation between oxygen radicals, membrane integrity, and calcium functions is indicated. | 1991 | 1855671 |
| the way we teach otolaryngology in an integrated problem-based curriculum. | the university science malaysia, penang, the third medical school in malaysia, is following an integrated curriculum. in this curriculum, the students are taught the subject of otolaryngology from the second year of their course unlike the traditional schools where they are taught in the fourth or fifth year. this paper describes how otolaryngology is introduced in this integrated, problem-based curriculum. | 2007 | 1865800 |
| transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. | methods for measuring plant cytoplasmic calcium using microelectrodes or microinjected fluorescent dyes are associated with extensive technical problems, so measurements have been limited to single or small groups of cells in tissue strips or protoplasts. aequorin is a calcium-sensitive luminescent protein from the coelenterate aequorea victoria (a. forskalea) which is formed from apoaequorin, a polypeptide of relative molecular mass approximately 22,000, and coelenterazine, a hydrophobic lumino ... | 2007 | 1865907 |
| allergic contact cheilitis from toothpastes. | 2007 | 1868726 | |
| expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco. | a possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. the ldcgene from hafnia alvei was therefore (a) placed under the control of the 1' promoter of the bidirectional tr promoter from agrobacterium tumefaciens ... | 1991 | 1883998 |
| trans-atlantic in vitro fertilization cold war. | 2007 | 1899401 | |
| a specific assay for serum intestinal alkaline phosphatase utilizing fluorescein labelled monoclonal antibody and antifluorescein antibody coupled to magnetic particles. | 2007 | 1907122 | |
| [analysis of kinetics of consecutive metabolic cerebral activations induced by spatial discrimination testing, using (14c)-glucose, in an eight-arm radial maze in mice]. | regional mapping of relative (14c)-glucose (glu) uptake was analyzed in balb/c mice at 3 time intervals (5 min., 1 hr., 3 hrs.) after either the first (day 1) or the last (day 9) daily sessions of a spatial discrimination testing procedure in an eight-arm radial maze. on day 1, increased labelling was found 5 min. post-training in subcortical, hippocampal and cortical regions. decreased glu uptake was observed 1 hr. later in the same regions, followed at 3 hrs. post-training by a retarded activa ... | 2007 | 1913258 |
| coronary vasospasm as a potential cause of myocardial infarction and paroxysmal atrial fibrillation in a relatively young woman. | vasospasm-related myocardial infarction in young women with normal coronary arteries has infrequently been reported and vasospasm-related paroxysmal atrial fibrillation (paf) has rarely been described. we present a 33-year-old woman with old inferior myocardial infarction and postinfarction angina at rest; the angina was accompanied by paf and electrocardiographic st-segment elevation in the inferior leads. coronary angiography revealed normal coronary arteries and intracoronary acetylcholine pr ... | 2007 | 1914277 |
| esp3i--a novel type iis restriction endonuclease from hafnia alvei that recognizes the sequence 5'-cgtctc(n)1/5-3'. | 1991 | 1923776 | |
| l-prolineaminopeptidase activity as a tool for identification and differentiation of serratia marcescens, serratia liquefaciens and hafnia alvei strains. | s. marcescens (316 strains), s. liquefaciens (10 strains) and hafnia alvei (20 strains), in contrast to 18 other enterobacteriaceae species, hydrolyzed l-proline-4-nitroanilide within 30 min at 37 degrees c. in this way, rapid identification of these species is possible. the substrate is applied in solution or in a paper disc. the substrate optimum of hafnia alvei strains proved to be 16 times lower than that of the serratia spp. application of a tenth of the substrate concentration necessary fo ... | 1991 | 1930558 |
| reactive modifications of the autonomous time structure in the human organism. | the spectrum of biological rhythms exhibits characteristic principles of biological time structure which also rule the functional behaviour. with increasing period lengths the rhythms become increasingly complex. in the long-wave section the rhythmic functions find their corresponding cycles in the environment, whereas the shorter waves represent only endogenous autonomous rhythms, which maintain an internal time order by means of frequency- and phase-coordination. under resting conditions and i ... | 1991 | 1932773 |
| etiologic role of hafnia alvei in human diarrheal illness. | 1991 | 1937837 | |
| induction of the alka gene of escherichia coli in gram-negative bacteria. | a broad-host-range plasmid containing a fusion of the alka and lacz genes of escherichia coli was introduced into various aerobic and facultative gram-negative bacteria--33 species belonging to 19 genera--to study the induction of expression of the alka gene by alkylating agents. the bacteria included species of the families enterobacteriaceae, pseudomonadaceae, rhizobiaceae, vibrionaceae, neisseriaceae, rhodospirillaceae, and azotobacteraceae. results obtained show that all bacteria tested, exc ... | 1991 | 1938974 |
| the prognostic value of exercise capacity: a review of the literature. | while there is still much debate in the literature regarding the specific met levels at which there are differences in survival, the following points have become clear with the growing body of reports in the literature. exercise capacity seems to be an independent predictor of mortality, and when it is combined with other clinical, exercise, or angiographic data, it becomes very powerful in this regard. this relates to both overall mortality and to that from cardiovascular disease. there is stil ... | 2007 | 1951007 |
| pathogenicity and antigenic components of excysted giardia lamblia isolated from patients in riyadh, saudi arabia. | giardia lamblia cysts were isolated from patients in riyadh, saudi arabia. cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the riyadh isolate. there was no effect of varying the dose of administered parasite on parasite excretion or morbidity. a typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. antigenic components of the riyadh isolate were compared with the portl ... | 1991 | 1951852 |
| production and characterization of a monoclonal antibody specific for enterohemorrhagic escherichia coli of serotypes o157:h7 and o26:h11. | a monoclonal antibody (mab 4e8c12) specific for escherichia coli o157:h7 and o26:h11 was produced by immunizing balb/c mice with a rough strain of e. coli o157:h7. the antibody reacted strongly by a direct enzyme-linked immunosorbent assay with each of 36 strains of e. coli o157:h7. no cross-reactivity was observed with strains of salmonella spp., yersinia enterocolitica, shigella dysenteriae, proteus spp., escherichia hermanii, klebsiella pneumoniae, campylobacter jejuni, serratia marcescens, c ... | 1991 | 1993773 |
| hafnia alvei, a probable cause of diarrhea in humans. | hafnia alvei, a member of the family enterobacteriaceae, was the only species of bacteria cultured from the stool of a 9-month-old child who was admitted with a 3-day history of watery diarrhea. the isolated strain of h. alvei failed to produce heat-labile or heat-stable enterotoxins or shiga-like toxin i or ii and did not invade hela cells, nor did it cause keratoconjunctivitis (determined by the sereny test) in a guinea pig's eye. the strain, however, induced diarrhea in 8 of 12 adult rabbits ... | 1991 | 2004829 |
| structure of the o-specific, sialic acid containing polysaccharide chain and its linkage to the core region in lipopolysaccharide from hafnia alvei strain 2 as elucidated by chemical methods, gas-liquid chromatography/mass spectrometry, and 1h nmr spectroscopy. | mild acid hydrolysis of hafnia alvei strain 2 lipopolysaccharide released no o-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with n-acetylneuraminic acid as the reducing terminus. in addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fragment of the o-specific polysaccharide chain and the core region, were obtained in minute amounts. on the basis of the sugar and methylation analyses, periodate oxidation, and 1h nm ... | 1991 | 2036370 |
| are we contributing to the "me" generation? | 2007 | 2043296 | |
| identification of the bacterium associated with haemorrhagic septicaemia in rainbow trout as hafnia alvei. | an unidentified bacterium responsible for epizootic haemorrhagic septicaemic disease in rainbow trout was recently described. this organism exhibited some antigenic similarity to brucella abortus and to yersinia ruckeri and was presumed to belong to the family enterobacteriaceae. further biochemical characterization and dna relatedness studies showed that pathogenic fish strains are hafnia alvei. | 1990 | 2120748 |
| immunogenic and protective properties of outer membrane proteins of hafnia alvei. | outer membrane proteins (omp) extracted from antigenically distinct or related strains of hafnia alvei containing defined composition of major proteins proved to be immunogenic. intraperitoneal immunization of mice with a single dose of such preparations protected the animals against homologous and heterologous hafnia strains. the omp preparations were also found to induce protection with varying intensity against escherichia, proteus, shigella and salmonella. | 1990 | 2130804 |
| the normal microbial flora of the outer ear canal in healthy norwegian individuals. | the microbial flora of the outer ear canal was determined for 77 healthy individuals (m = 44, f = 33). no growth of any microbe was found in 5% of males and 15% of females. coagulase-negative staphylococci, dominated by staphylococcus epidermidis, were the commonest microbe group found (83% of persons sampled). staphylococcus aureus was found in 7% males and no females. diptheroids were cultured from 32% of the samples. the only gram-negative rod found was hafnia alvei in 4% of individuals. no v ... | 1990 | 2216049 |
| [typical nosocomial infection with an unusual cause: hafnia alvei. report of 2 cases and literature review]. | the route of infection and the course of two typical nosocomial infections are described in two patients infected with a rare gram-negative bacterium. both patients underwent cardiovascular surgery. they were placed close to each other in the intensive care unit for several days and suffered from pneumonia and from wound infection respectively. in both patients bacterial culture grew hafnia alvei. successful antibiotic treatment was achieved with netilmicin and imipenem. urinary tract, respirato ... | 1990 | 2218242 |
| [disorders in the sequence of regional metastasis and long-term results of the surgical treatment of complicated lung cancer]. | to ascertain the frequency of disturbance of the stage character of regional metastasis in lung carcinoma complicated by disintegration or obstructive pneumonitis, and its influence on certain results of surgical treatment, the authors analysed the data on 175 patients who underwent lung resection. the stage character of regional lymphogenous metastasis was found to be disturbed in 61 (34.8%) patients, in 52 (85.2%) of them the main disease was complicated by obstructive pneumonitis. five-year s ... | 2007 | 2223212 |
| [disorders in the sequence of regional metastasis and long-term results of the surgical treatment of complicated lung cancer]. | to ascertain the frequency of disturbance of the stage character of regional metastasis in lung carcinoma complicated by disintegration or obstructive pneumonitis, and its influence on certain results of surgical treatment, the authors analysed the data on 175 patients who underwent lung resection. the stage character of regional lymphogenous metastasis was found to be disturbed in 61 (34.8%) patients, in 52 (85.2%) of them the main disease was complicated by obstructive pneumonitis. five-year s ... | 2007 | 2223212 |
| [criteria for the selection of patients with a single heart ventricle for anatomical correction]. | the work deals with study of the functional condition of the myocardium in 34 patients with a solitary ventricle of the heart (svh). the regional function of the myocardium was disturbed in all patients; the degree of the disturbance was determined by the anatomical variant of the svh and was characterized by different distribution of the hypofunctioning segments in the ventricular myocardium. in view of that, to solve the problem of the possibility of accomplishing anatomical correction, the re ... | 2007 | 2223228 |
| purification of cellular retinoic acid-binding protein from human placenta. | 1990 | 2292943 | |
| endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. | enteric pathogens rarely involve organs other than those of the gastrointestinal system. we have reported the case of a woman with rheumatoid arthritis who had endogenous endophthalmitis due to salmonella arizonae and hafnia alvei. the infection probably resulted from the use of snake powder as a food seasoner. after appropriate intravenous, intraocular, subconjunctival, topical, and oral antibiotic therapy based on laboratory susceptibility studies, the patient's condition clinically improved, ... | 1990 | 2346566 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| the sugar moiety of bovine s-antigen. | to obtain insight into the glycoprotein nature of s-antigen (s-ag), we have investigated the affinity of bovine s-ag for plant lectins, location of the sugar moiety within the molecule, and incorporation of radiolabelled mannose and glucosamine into s-ag. it was found (i) that only about 10% of purified s-ag was bound to concanavalin a (con a) and wheat germ agglutinin (wga) columns, (ii) that both concanavalin a and wheat germ agglutinin bound chymotryptic peptides derived from the c-terminal h ... | 1990 | 2384003 |
| hafnia alvei infection. | 1990 | 2402660 | |
| [a test for the identification of hafnia alvei bacteria by the color reaction with a complex of 5-nitro-8-hydroxyquinoline + iron ions]. | 1986 | 2429042 | |
| theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism. | the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ... | 1987 | 2446888 |
| [role of plastic surgery in male sterility]. | the authors discuss the problems related to male infertility, pointing out their different clinical and therapeutical features. they stress the importance of plastic surgery in the treatment of disorders such as penile chordee, hypospadias as well as nervous and vascular lesions. | 2008 | 2518539 |
| [importance of peptide c determination in diabetes]. | the peptide c, polypeptide secreted by the pancreas at the same time as insulin, presents a great interest in the evaluation of diabetic patients. first it allows a differentiation between insulin dependent diabetes (idd) and non insulin dependent (ndd). a low and non stimulated levels of peptide c signifies an insulin dependence. within the group of idd patients the peptide c was low when the diabetes was discovered at a younger age and its secretion diminished as the diabetes progresses. the p ... | 2008 | 2519329 |
| [medical leave in hospital personnel]. | we investigated medical leaves of personnel at temuco's hospital from 1986 to 1987. 57% of persons had at least one leave and 54% of these had 2 or more leaves. women and paramedicals had more medical leaves. most absences occurred in june, july, august (p less than 0.01). most leaves were caused by respiratory diseases (26%) with absence periods from 1 to 6 days. infectious and parasitic diseases caused absences ranging from 1 to 42 days. better health policies may help decrease this rather hig ... | 2008 | 2519440 |
| sequences that promote formation of catenated intertwines during termination of dna replication. | the normal sequence at which sv40 dna replication terminates (ter) is unusual in that it promotes formation of catenated intertwines when two converging replication forks enter to complete replication (weaver et al., 1985). here we show that yeast centromeric sequences also exhibit this phenomenon. cen3 caused accumulation of late replicating intermediates and catenated dimers in plasmids replicating in mammalian cells, but only when it was located in the termination region (180 degrees from ori ... | 1989 | 2542894 |
| [thermometric diagnostics of chronic ulcerous pulpitis]. | thermometric measurements were performed of 408 teeth of the upper and lower jaw with clinically confirmed initial and advanced stage of chronic ulcerous pulpitis in 398 subjects, aged from 18 to 30. temperature elevation within the range from 1.4 to 3.2 degrees c was established in the initial form of chronic pulpitis and from 1 to 2.5 degrees c in advanced form of chronic pulpitis as compared with the norm. significant difference in the temperature deviations exists in both forms of chronic pu ... | 2007 | 2562574 |
| patients with neuroleptic malignant syndrome histories: what happens when they are rehospitalized? | among approximately 1450 patients treated with neuroleptics in a short-term psychiatric hospital, there were no cases of the neuroleptic malignant syndrome (nms). six patients with histories of nms were treated, either with nonneuroleptic therapies or with low doses of low-potency antipsychotic agents. no case of nms developed, but one patient suffered transient subsyndromal signs while treated briefly with loxapine. staff education, screening for patients with history of nms, and detection of e ... | 2008 | 2565897 |
| cardiac hypersensitivity to 5-aminosalicylic acid. | 2008 | 2566070 | |
| comparison of a vasodilating beta-blocker and a cardioselective beta-blocker in long-term treatment of hypertension: a european multicentre study. | the therapeutic effectiveness and safety of indenolol, a vasodilating beta-blocker with beta 2-agonism, was compared with that of atenolol, a cardioselective beta-blocker, in a 1-year double-blind trial. a total of 143 hypertensive patients (diastolic blood pressure 95-115 mmhg after 1 month of placebo) were randomly allocated to either atenolol, 50 mg/day, or indenolol, 60 mg/day. if the target diastolic blood pressure (less than or equal to 90 mmhg) was not reached after 1 month, the beta-bloc ... | 2008 | 2576667 |
| evaluation of n-(5-indanylsulfonyl)-n'-(4-chlorophenyl)-urea against xenografts of pediatric rhabdomyosarcoma. | n-(5-indanylsulfonyl)-n'-(4-chlorophenyl)-urea (ly186641), a novel anticancer compound, was evaluated against six lines of rhabdomyosarcoma xenografts, each of which was established from tissue biopsies from untreated patients, and additional sublines selected as xenografts for primary resistance to vincristine, melphalan, and ifosfamide. ly186641 was given by oral gavage twice daily for 10 consecutive days or as 5-day courses repeated at 7-day intervals. at the optimal schedule, complete regres ... | 2008 | 2598403 |
| o-specific polysaccharides of hafnia alvei lipopolysaccharides isolated from two serologically related strains: atcc 13337 and 1187. a serological and structural study using chemical methods, gas chromatography/mass spectrometry and nmr spectroscopy at 500 mhz. | the o-specific polysaccharides of hafnia alvei atcc 13337 standard strain and 1187 strain have been isolated and characterized. by means of 1h-nmr spectroscopy, methylation analysis and periodate oxidation, the repeating unit of the polysaccharides could be allocated the respective structures. (formula; see text) where acyl = d-3-hydroxybutyryl, and 3-o-acetylation was to about 66%. the structural similarity of the polysaccharides was confirmed in the serological study; their epitopes were deter ... | 1989 | 2606108 |
| alkaline phosphatase in cholestatic and cirrhotic rats. a biochemical and histochemical study. | alkaline phosphatase (alp) was studied by enzyme histochemical methods and by biochemical quantitations in rat livers with chronic bile duct obstruction and experimental cirrhosis. the most evident alp increase was histochemically found in portal tracts of rats with bile duct obstruction and localized to the walls of proliferating blood vessels. furthermore, a slight canalicular membrane enzyme increase was histochemically found in both groups, most evident in cirrhosis, whereas the biochemical ... | 1989 | 2612454 |
| alkaline phosphatase in cholestatic and cirrhotic rats. a biochemical and histochemical study. | alkaline phosphatase (alp) was studied by enzyme histochemical methods and by biochemical quantitations in rat livers with chronic bile duct obstruction and experimental cirrhosis. the most evident alp increase was histochemically found in portal tracts of rats with bile duct obstruction and localized to the walls of proliferating blood vessels. furthermore, a slight canalicular membrane enzyme increase was histochemically found in both groups, most evident in cirrhosis, whereas the biochemical ... | 1989 | 2612454 |
| [variability of anthropometric traits at birth in healthy children and children suffering from acute respiratory diseases in the first year of life]. | variation of 5 anthropometric traits at birth in groups of normal children who had suffered from acute respiratory diseases during their first year of life was studied in russian migrant and native population inhabiting the western area of baikal-amur railway. it was shown that affected children from migrant and native population had lower average values of all traits, as compared with normal children. in order to reduce the number of variable, the principal component analysis was applied. stron ... | 2008 | 2620813 |
| [variability of anthropometric traits at birth in healthy children and children suffering from acute respiratory diseases in the first year of life]. | variation of 5 anthropometric traits at birth in groups of normal children who had suffered from acute respiratory diseases during their first year of life was studied in russian migrant and native population inhabiting the western area of baikal-amur railway. it was shown that affected children from migrant and native population had lower average values of all traits, as compared with normal children. in order to reduce the number of variable, the principal component analysis was applied. stron ... | 2008 | 2620813 |
| bilateral torsion of the normal ovary and oviduct in a young girl. | 2008 | 2643670 | |
| fabrication of a teaching aid for dental soft tissue management and suturing. | a technique for fabrication of a model to aid in teaching surgical management and suturing is presented. the model, which simulates dentition, supporting periodontium, and gingival soft tissue, can be made inexpensively in a dental laboratory. | 2008 | 2646346 |