Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| amino acid sequence of alcohol dehydrogenase from the thermophilic bacterium thermoanaerobium brockii. | the complete amino acid sequence of alcohol dehydrogenase of thermoanaerobium brockii (tbad) is presented. the s-carboxymethylated protein was cleaved at methionine residues (with cyanogen bromide) to provide a set of 10 nonoverlapping fragments accounting for 90% of the sequence. these fragments were then overlapped and aligned, and the sequence was completed by using peptides generated by proteolytic cleavage at lysine residues (with achromobacter protease i). the protein subunit contained 352 ... | 1989 | 2790012 |
| achromobacter xylosoxidans corneal ulcer in a therapeutic soft contact lens wearer. | achromobacter xylosoxidans is an opportunistic organism that is usually seen in immunocompromised or immunosuppressed patients. it is an aerobic gram-negative rod, often confused with other more commonly seen gram-negative bacteria such as pseudomonas aeruginosa. the organism is usually sensitive to extended spectrum penicillins such as carbenicillin and usually resistant to aminoglycosides and first generation cephalosporins. we wish to describe a corneal ulcer from a. xylosoxidans that develop ... | 1989 | 2805715 |
| bacterial synthesis of recombinant alpha-human atrial natriuretic polypeptide. | the high-level synthesis of alpha-human atrial natriuretic polypeptide hormone in escherichia coli has been achieved based on the idea that the yield of a small, basic and unstable polypeptide, such as the natriuretic polypeptide, would be improved by fusion with an appropriate protective polypeptide to construct a neutral fused polypeptide. we prepared an expression vector, pclahtrp3t, coding a neutral polypeptide containing 130 amino acid residues in which the polypeptide hormone was fused to ... | 1987 | 2822675 |
| complete amino acid sequence of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. | the complete amino acid sequence of 6-phospho-fructo-2-kinase/fructose-2,6-bisphosphatase from rat liver was determined by direct analysis of the s-carboxamidomethyl protein. a complete set of nonoverlapping peptides was produced by cleavage with a combination of cyanogen bromide and specific proteolytic enzymes. the active enzyme is a dimer of two identical polypeptide chains composed of 470 amino acids each. the nh2-terminal amino acid residue of the polypeptide chain was shown to be n-acetyls ... | 1988 | 2826464 |
| characterization of the major protein-tyrosine-phosphatases of human placenta. | in the preceding article (tonks, n. k., diltz, c. d., and fischer, e. h. (1988) j. biol. chem. 263, 6722-6730), the purification of the major protein-tyrosine-phosphatases from human placenta, some to apparent homogeneity, was described. this report compares the characteristics of these enzymes and clearly identifies at least two distinct protein-tyrosine-phosphatase catalytic subunits. all were absolutely specific for phosphotyrosyl residues and showed no activity on any of the phosphoseryl/pho ... | 1988 | 2834387 |
| bacterial collagenase and collagen identification. | a method is described for the cleavage of collagenous molecules by bacterial collagenase in the presence of sodium dodecyl sulphate (sds) and urea. comparison of three commercially available preparations of bacterial collagenase showed that the most efficient cleavage under these conditions was by the enzyme isolated from achromobacter iophagus (e.c. 3.4.24.8). no non-specific proteinase activity was apparent in conditions where all collagen types showed some susceptibility to attack. this metho ... | 1988 | 2835204 |
| near stoichiometric, irreversible inactivation of bacterial collagenases by o-chloranil (3,4,5,6-tetrachloro-1,2-benzoquinone). | the hydrogen-abstracting quinone derivative 3,4,5,6-tetrachloro-1,2-benzoquinone (o-chloranil) caused a strong, near stoichiometric, irreversible inactivation of the collagenases from bacillus cereus, clostridium histolyticum and achromobacter iophagus. p-chloranil was a weaker inactivator. o-chloranil reacted rapidly with a site that affected substrate binding. amino acid analyses of native and totally inactivated enzymes, and the ph-profile of inactivation suggest that the dissociated form of ... | 1988 | 2837215 |
| nitric oxide complex of cytochrome c' in cells of denitrifying bacteria. | electron paramagnetic resonance (epr) spectra at 77k have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: i, denitrifying bacteria containing cytochrome c' (alcaligenes sp. ncib 11015, achromobacter xylosoxidans gifu 543); ii, denitrifying bacteria in which cytochrome c' has not been found (alcaligenes faecalis iam 1015, bacillus firmus nias 237, pseudomonas stutzeri atcc 17641); iii, non-denitrifying bacterium containing cyto ... | 1988 | 2844743 |
| a spectrophotometric assay for dissimilatory nitrite reductases. | a spectrophotometric assay for dissimilatory nitrite reductases has been developed utilizing mammalian cytochrome c (equine heart) as reductant and spectrophotometric agent. the copper-containing nitrite reductase from achromobacter cycloclastes has been shown to have apparent km's for reduced cytochrome c and nitrite of 86 +/- 5 and 5.63 +/- 0.03 microm, respectively. the heme cd-containing enzyme from pseudomonas stutzeri was shown to have apparent km's for reduced cytochrome c and nitrite of ... | 1988 | 2847585 |
| [the biogenetic interrelationships between flavins and vitamin b12 in achromobacter cobalamini]. | 1988 | 2854877 | |
| prevention of bacterial contamination of water in dental units. | a study of the bacterial contamination of water in dental units was conducted in five dental practitioners' clinics and one university dental hospital in tokyo. various bacteria in numbers of 10(2)-10(5)/ml were detected in the water delivered from all 42 of the dental units examined, though very few bacteria were detected in the tap water upstream of the units. most of the organisms isolated from the water were gram-negative bacilli, predominantly alcaligenes, achromobacter and pseudomonas spp. ... | 1985 | 2859327 |
| osteomyelitis secondary to trauma or infected contiguous soft tissue. | nonhematogenous osteomyelitis (nho) occurred in 24 pediatric patients (ages 8 months to 18 years; median, 14 years; 23 male) admitted from 1980 to 1985. predisposing factors included compound fracture (12), deep decubiti (4) and foot puncture (3). infection involved tibia (7), foot bones (6), proximal femur (3) and ulna (2). patients presented with drainage (64%), pain or tenderness (44%) and fever (32%) lasting for 1 to 180 days (median, 10 days). in 24% both white blood cell count and erythroc ... | 1988 | 2893333 |
| primary structure of chicken liver acetyl-coa carboxylase deduced from cdna sequence. | the complete amino acid sequence of acetyl-coa carboxylase from chicken liver has been deduced by cloning and sequence analysis of dna complementary to its messenger rna. the results were confirmed by edman degradation of peptide fragments obtained by digestion of the enzyme polypeptide with achromobacter proteinase i or staphylococcal serine proteinase. chicken liver acetyl-coa carboxylase is predicted to be composed of 2,324 amino acid residues, having a calculated molecular weight of 262,706. ... | 1988 | 2893793 |
| outbreak of infection with achromobacter xylosoxidans from contaminated intravascular pressure transducers. | achromobacter xylosoxidans contaminating transducers caused 15 cases of hospital infection. in the eight patients with bacteraemia the interval from inoculation to fever was an average of 6.6 days. all the infected patients recovered. computerization of laboratory records allowed retrieval of previous isolates, and review of clinical records focused the problem on patients with cardiac and aortic diseases. the problem arose from the re-use of disposable equipment after disinfection with a benzal ... | 1988 | 2905368 |
| in-vitro activity of newer quinolones against aerobic bacteria. | nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ... | 1986 | 2940214 |
| electron paramagnetic resonance studies of heme c and its nitrosyl derivative in vibrio (achromobacter) fischeri nitrite reductase. | interactions of vibrio (formerly achromobacter) fischeri nitrite reductase were studied by electron paramagnetic resonance spectroscopy. the spectrum of the oxidized enzyme showed a number of features which were attributed to two low-spin ferric hemes. these comprised an unusual derivative peak at g = 3.7 and a spectrum at g = 2.88, 2.26, and 1.51. neither heme was reactive in the oxidized state with the substrate nitrite and with cyanide and azide. when frozen under turnover conditions (i.e., r ... | 1986 | 3013249 |
| purification and some properties of cytochrome c' from a strain of achromobacter xylosoxidans. | cytochrome c' was crystallized from achromobacter xylosoxidans gifu 543. the cytochrome was a basic protein and its molecular weight was 28,000. the pyridine ferrohemochrome showed absorption peaks at 415, 521, and 551 nm. the absorption spectra of the oxidized and reduced forms at neutral ph were almost the same as those of other cytochromes c' reported already. the reduced cytochrome c' reacted with co and no, and the no complex showed a characteristic absorption spectrum. the midpoint redox p ... | 1986 | 3017925 |
| [peptide inhibitors of a bacterial collagenase]. | the properties of the cleavage site of collagen by collagenases remain discussed. the authors report their studies on the active site of the collagenase of a bacteria, achromobacter, based on two types of methods. the first type uses synthetic substrates, the second one enzymatic inhibitors. the results of both methods are reviewed. the inhibitors seem more sensitive than substrates to study the relation between structure and activity of the enzyme. | 1986 | 3019185 |
| new thiol inhibitor of achromobacter iophagus collagenase. specificity of the enzyme's s3' subsite. | new synthetic mercaptotripeptides (hs-ch2-ch2-co-pro-yaa) which inhibit achromobacter iophagus collagenase were produced in order to obtain more powerful bacterial collagenase inhibitors than currently available, and to investigate the specificity of the s3' subsite of the enzyme. since similar binding constants were found for inhibitors carrying uncharged residues of various sizes in the p3' position (yaa = ala, leu, phe, pro, hyp) steric hindrance at the collagenase s3' appears relatively limi ... | 1986 | 3021459 |
| detergent inhibition of nitric-oxide reductase activity. | gas chromatography revealed that exposure of extracts of the denitrifiers 'achromobacter cycloclastes', paracoccus denitrificans, pseudomonas aeruginosa and pseudomonas perfectomarina to triton x-100 inhibited reduction of no to n2o, and thus concomitantly inhibited reduction of no2- to n2o. after exposure of extracts to triton x-100, the ratio of h+ consumed to no2- added decreased from approx. 2.0 (for untreated extracts) to approx. 1.5, which indicated that no2- was reduced to no by the treat ... | 1987 | 3028488 |
| generation of peptides suitable for sequence analysis by proteolytic cleavage in reversed-phase high-performance liquid chromatography solvents. | a methodological study of practical importance to protein sequencing has been carried out. peptide mapping and sequence analysis of the cleavage products of reduced and carboxymethylated ribonuclease have been applied to the study of the activity and specificity of trypsin, chymotrypsin, elastase, lysyl endopeptidase (achromobacter protease i), endoproteinase arg-c (from mouse submaxillary gland), staphylococcus aureus v8 protease, pepsin, and thermolysin in the presence of 20% methanol, ethanol ... | 1988 | 3064654 |
| identity of molecular structure of shiga-like toxin i (vt1) from escherichia coli o157:h7 with that of shiga toxin. | the primary structures of the a and b subunits of shiga toxin and of shiga-like toxin i (vt1), isolated from the culture supernatants of shigella dysenteriae 1 and escherichia coli o157:h7, respectively, were analyzed by edman degradation of intact proteins and peptides in their digests with trypsin or achromobacter protease i and also by fast atom bombardment mass spectrometry of the digests. the results indicated that the a and b subunits of shiga toxin and shiga-like toxin i have the same pri ... | 1988 | 3070268 |
| influence of temperature on the enzymic semisynthesis of human insulin by coupling and transpeptidation methods. | the influence of temperature of enzymic semisynthesis of human insulin ester was determined by using coupling and transpeptidation methods with trypsin and achromobacter lyticus proteinase i as catalysts. the optimal reaction conditions were studied at the selected temperatures of 25, 12 and 4 degrees c. the results showed that the synthesis rates by both methods with trypsin increased as the temperature increased, but the final product yield correspondingly decreased. therefore the reaction wit ... | 1986 | 3103612 |
| emerging microorganisms in cystic fibrosis. | pseudomonas aeruginosa is the most common bacterial isolate obtained from patients with cystic fibrosis of the lungs. recently, however, new multiresistant organisms have emerged, whose identification may be difficult and whose pathogenic role proves hard to define. of the 71 strains isolated from 24 patients with cystic fibrosis during acute flareups of pulmonary symptoms, 48 turned out to be pseudomonas aeruginosa (67.6%); 11 were pseudomonas non-aeruginosa (15.5%); and 12 were achromobacter x ... | 1987 | 3103930 |
| resistance to biological and chemical challenge in rodents treated with xerosin ii, a natural product of achromobacter xerosis. | the biological response-modifying activity of acid-precipitable material from achromobacter xerosis was first described as suppression of viral pneumonia in mice. later, this acid-precipitable material (xerosin) was found to have antiinflammatory activity and to induce tumor regression in chickens infected with rous sarcoma virus. here, we report further purification of xerosin resulting in a product (xerosin ii) that retains high biological activity against viral and endotoxin-induced pneumonia ... | 1987 | 3108461 |
| chronic bacterial endophthalmitis. | we studied a specific syndrome of uveitis secondary to intraocular bacterial pathogens of low virulence after extracapsular cataract extraction and intraocular lens implantation in three eyes. the onset of photophobia, visual impairment, conjunctival redness, and uveitis was delayed for four days to 12 weeks after surgery. chronic inflammation persisted for five weeks to 16 months before a definitive diagnosis was made. signs and symptoms were suppressed by administration of topical and systemic ... | 1987 | 3109246 |
| a phospholipase a2 in the supernatant fraction of rat spleen. its similarity to rat pancreatic phospholipase a2. | rat spleen supernatant contained two forms of calcium-dependent cellular phospholipase a2 which could be separated from each other by teae-cellulose chromatography. the phospholipase a2, named pla2 s-1, present in the major flow-through fraction was purified to homogeneity. the structural and catalytic properties of splenic pla2 s-1 were systematically compared with those of rat pancreatic phospholipase a2. structural evidence, including the sequence of the n-terminal 32 residues, peptide maps o ... | 1988 | 3128547 |
| primary structure of sheep prostaglandin endoperoxide synthase deduced from cdna sequence. | the complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of dna complementary to its messenger rna. the results were confirmed by digestion of the enzyme with carboxypeptidase y and by automated edman degradation of the intact enzyme polypeptide and peptide fragments obtained by limited proteolysis of the enzyme with achromobacter proteinase i. mature sheep prostaglandin endoperoxide synthase is shown to ... | 1988 | 3129310 |
| comparative in vitro activity of the new oral cephalosporin cefixime. | cefixime was 8 to 10 times more active than cefaclor and augmentin against isolates of escherichia coli, klebsiella pneumoniae and salmonella typhi, mic90 values ranging from 0.06 to 0.25 micrograms/ml. however, none of these three drugs was particularly active against isolates of more resistant gram-negative bacilli such as enterobacter, serratia, citrobacter, acinetobacter, providencia and achromobacter spp. the lowest mic values for gram-negative bacilli were seen with ciprofloxacin, except f ... | 1988 | 3137053 |
| evaluation of 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) in broth and agar media for identification of pseudomonas aeruginosa. | brain heart infusion broth and mueller-hinton agar containing the compound 9-chloro-9-[4-(diethylamino)phenyl]- 9,10-dihydro-10-phenylacridine hydrochloride (c-390) were evaluated as selective media for identifying pseudomonas aeruginosa. of the 192 pseudomonas spp. and 68 additional oxidase-positive or glucose-nonfermenting gram-negative organisms tested, only p. aeruginosa (120 of 121 isolates) and certain strains of pseudomonas cepacia, aeromonas hydrophila, and achromobacter xylosoxidans wer ... | 1988 | 3141473 |
| microbial transformation of the pyrethroid insecticides: permethrin, deltamethrin, fastac, fenvalerate, and fluvalinate. | pure cultures of bacillus cereus, pseudomonas fluorescens, and achromobacter sp. were shown to transform five pyrethroid insecticides in the presence of tween 80. one of the major products, 3-phenoxybenzoic acid, was further transformed to 4-hydroxy-3-phenoxybenzoic acid. permethrin was the most rapidly transformed of the pyrethroids, with a half-life of less than 5 days. | 1988 | 3145715 |
| amino-acid sequence of ribonuclease t2 from aspergillus oryzae. | the amino acid sequence of ribonuclease t2 (rnase t2) from aspergillus oryzae has been determined. this has been achieved by analyzing peptides obtained by digestions with achromobacter lyticus protease i, staphylococcus aureus v8 protease, and alpha-chymotrypsin of two large cyanogen bromide peptides derived from the reduced and s-carboxymethylated or s-aminoethylated protein. digestion with a. lyticus protease i was successfully used to degrade the n-terminal half of the s-aminoethylated prote ... | 1988 | 3169020 |
| amino acid sequence of a coagulant enzyme, flavoxobin, from trimeresurus flavoviridis venom. | the amino acid sequence of a coagulant enzyme, named flavoxobin, isolated from the venom of trimeresurus flavoviridis (the habu snake) was determined by sequencing the s-pyridylethylated derivative of the protein and its peptides generated by chemical (cyanogen bromide and hydroxylamine) and enzymatic (clostripain, staphylococcus aureus v8 protease, achromobacter protease i, and elastase) cleavages. hydrazinolysis was also employed to determine the c-terminal amino acid. the enzyme consisted of ... | 1988 | 3170503 |
| resonance raman spectra of the copper-sulfur chromophores in achromobacter cycloclastes nitrite reductase. | resonance raman spectroscopy at ambient temperature and 77 k has been used to probe the structures of the copper sites in achromobacter cycloclastes nitrite reductase. this enzyme contains three copper ions per protein molecule and has two principal electronic absorption bands with lambda max values of 458 and 585 nm. comparisons between the resonance raman spectra of nitrite reductase and blue copper proteins establish that both the 458 and 585 nm bands are associated with cu(ii)-s(cys) chromop ... | 1988 | 3170560 |
| nosocomial outbreak of achromobacter xylosoxidans associated with a diagnostic contrast solution. | 1988 | 3170779 | |
| achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound. | the microbiological and clinical features of a case of achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound are described. to our knowledge, this is the third confirmed case report of meningitis caused by this organism. | 1988 | 3235669 |
| nosocomial achromobacter xylosoxidans infections. | a xylosoxidans is being recognized as an important nosocomial pathogen. as more patients are rendered immunosuppressed by chemotherapy, this organism's increasing role in hospital-acquired infections will be assured. achromobacter is a water-borne organism, highly resistant to most antibiotics, and even to some disinfectant solutions, and easily establishes itself in the hospital aquatic environment. achromobacter infections and outbreaks should be recognized and approached as serious problems r ... | 1988 | 3278052 |
| achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) bacteremia associated with a well-water source: case report and review of the literature. | a case of community-acquired achromobacter xylosoxidans bacteremia in a patient with metastatic breast carcinoma is described. the patient's home drinking water was identified as the source of her bacteremia. the case represents the first in which a community-acquired infection due to this organism has been attributed to a documented water source. | 1988 | 3281982 |
| amino acid sequence of sialic acid binding lectin from frog (rana catesbeiana) eggs. | the complete amino acid sequence of sialic acid binding lectin from frog (rana catesbeiana) egg is presented. the 111-residue sequence was determined by the analysis of peptides generated by digestion of the s-carboxymethylated protein with achromobacter protease i, chymotrypsin, or cyanogen bromide. the sequence is unique and not homologous to any known protein sequence. the protein may represent a new type of lectin. | 1987 | 3304421 |
| [treatment of post-traumatic and post-neurosurgical bacterial meningitis with ceftriaxone alone or in combination with fosfomycin]. | from 1984 to 1986, 13 patients (10 adults, 3 children) with bacterial meningitis following neurosurgery or traumatism were given ceftriaxone alone 6 times at a dose of 40 mg/kg one iv injection per day, or in association 7 times with fosfomycin at a dose of 200 mg/kg/day, 3 iv perfusions every 4 h. the bacteriological diagnosis was confirmed in 9 cases (3 staphylococcus aureus, 4 streptococcus pneumoniae, 1 klebsiella, 1 peptococcus). in vitro neither synergy nor antagonism were observed between ... | 1987 | 3309825 |
| use of immobilized achromobacter protease i for semisynthesis of human insulin. | 1987 | 3316927 | |
| achromobacter xylosoxidans bacteremia. | achromobacter xylosoxidans is a rare cause of bacteremia. a case of community-acquired pneumonia and bacteremia due to achromobacter in a patient with concomitant pulmonary tuberculosis is reported herein. the majority of patients who have developed achromobacter bacteremia have had a predisposition to infection (although the predisposing conditions have been diverse). immunosuppression has been reported in only one of the seven patients with achromobacter bacteremia for whom detailed informatio ... | 1987 | 3317727 |
| trapping of nitric oxide produced during denitrification by extracellular hemoglobin. | a spectrophotometric method has been developed that uses extracellular hemoglobin (hb) to trap nitric oxide (no) released during denitrification as nitrosyl hemoglobin (hbno). the rate of complexation of no with hb is about at the diffusion controlled limit for protein molecules and the product, hbno, is essentially stable. hb was added to an anaerobic bacterial suspension and denitrification was initiated with either kno2 or kno3. hbno formation was observed for six species of denitrifying bact ... | 1988 | 3339013 |
| studies on ampicillin resistance in achromobacter xylosoxidans. brief report. | population analyses of susceptibility to ampicillin in ampicillin-susceptible and ampicillin-resistant strains of achromobacter xylosoxidans revealed the existence of ampicillin-resistant subpopulations in ampicillin-susceptible isolates. bacteria resistant to a concentration four times the one that inhibited the majority population had a frequency of 10(-3) to 10(-4). strains isolated from aqueous environments are often found susceptible to ampicillin, while sporadic cases of infections with a. ... | 1988 | 3345263 |
| crystallization of nitrite reductase from achromobacter cycloclastes. | crystals of a green copper-containing nitrite reductase from achromobacter cycloclastes, which diffract to high resolution, belong to the cubic space group p213, with a = b = c = 98.4 a. crystals of a nitrite reductase from alcaligenes faecalis s-6 have been made, and belong to space group p212121, with a = 77.3 a, b = 94.6 a and c = 141 a. crystals of the blue copper protein from ac. cycloclastes have also been obtained: these belong to space group p21212, with cell dimensions a = 34.9 a, b = 9 ... | 1988 | 3373536 |
| chemical modification studies on achromobacter fischeri nitrite reductase. | 1987 | 3449441 | |
| amino acid sequence of the von willebrand factor-binding domain of platelet membrane glycoprotein ib. | we report the amino acid sequence of a 299-residue segment from the alpha chain of the human platelet membrane glycoprotein ib. this includes the complete sequence of the amino-terminal tryptic fragment of 290 residues comprising the von willebrand factor-binding domain. two primary sets of overlapping fragments were obtained by cleavage of the s-carboxymethylated protein at methionyl and lysyl bonds following treatment with cyanogen bromide and achromobacter protease i, respectively. additional ... | 1987 | 3497398 |
| the amino acid sequence of the blue copper protein of alcaligenes faecalis. | the complete amino acid sequence of a blue copper protein from alcaligenes faecalis s-6 has been determined. this protein is clearly homologous to pseudoazurins in achromobacter cycloclastes and pseudomonas am1, more distantly related to plant plastocyanins, and markedly different from the azurin of pseudomonas aeruginosa. yet all of these proteins bind copper, and analogous ligands appear to be involved. | 1986 | 3512305 |
| amino acid sequence of trimeresurus flavoviridis phospholipase a2. | the amino acid sequence of phospholipase a2 from the venom of trimeresurus flavoviridis (the habu snake) was determined. the enzyme subunit has a molecular weight of 13,764 and consists of a single polypeptide chain of 122 amino acids and seven disulfide bonds. the fragmentation was conducted by digesting the reduced and s-carboxymethylated derivative of the protein with achromobacter protease i, chymotrypsin, and trypsin, respectively. achromobacter protease i peptides were used for alignment a ... | 1986 | 3514593 |
| amino acid sequence of copper,zinc-superoxide dismutase from spinach leaves. | the complete amino acid sequence of cu,zn-superoxide dismutase (sod) from spinach leaves has been determined on the basis of peptides obtained by cyanogen bromide (brcn) cleavage and by enzymic hydrolyses with achromobacter lyticus lysylendopeptidase, staphylococcus aureus v8 protease, trypsin, and thermolysin. the spinach sod consists of a total of 154 amino acid residues with alanine as the amino(n)-terminus and valine as the carboxy(c-)terminus. the present sequence, which has been establishe ... | 1986 | 3519601 |
| [application of a micromethod to the study of the bactericidal activity of 2 antiseptics based on chlorhexidine gluconate]. | minimal bactericidal concentrations (mbcs) of two antiseptics containing chlorhexidine digluconate (5% for the a solution, 1.5% for the b solution) were determined using a microdilution method. this method is a miniaturization of the afnor t 72150 standard for antiseptics and disinfectants. 133 hospital isolates were studied, including 68 enterobacteriaceae, 11 acinetobacter, 18 pseudomonas, 1 achromobacter, 23 staphylococcus, and 12 s. faecalis. each microplate was seeded with eleven of these s ... | 1986 | 3534769 |
| enzymatic semisynthesis of [leub30] insulin. | experimental conditions for the preparation of [leub30] insulin by coupling of des-alab30 insulin with leu-obu(t) were determined using achromobacter protease i and trypsin as catalysts. successful coupling required a large excess of the amine component (0.8 m), a high concentration of organic cosolvent (35-50%) and neutral ph of the reaction mixture. the coupling yield of achromobacter protease i after 24 h at 37 degrees c was almost the same or a little higher than that at 25 degrees c. with t ... | 1986 | 3539841 |
| amino acid sequence of thermostable direct hemolysin produced by vibrio parahaemolyticus. | the complete amino acid sequence of the subunit of thermostable direct hemolysin, a dimeric protein composed of identical subunits isolated from vibrio parahaemolyticus, was determined by sequencing brcn-peptides, their tryptic peptides, and overlaps obtained by achromobacter protease i digestion. the subunit consists of 165 amino acid residues with the sole disulfide bond between cys 151 and cys 161. it is deduced that the biologically active hemolysin is formed by noncovalent association of su ... | 1987 | 3571196 |
| kinetic studies of the copper nitrite reductase from achromobacter cycloclastes and its interaction with a blue copper protein. | transient state, burst and steady state kinetics of reactions of the blue copper nitrite reductase (nir) and blue copper protein from achromobacter cycloclastes are investigated. the two copper-containing species are reacted with each other and where possible with dithionite, ascorbate and nitrite. both copper proteins are fully reduced by dithionite with both s2o4(2-) and so2-. species active. nir is only partially reduced by ascorbate in an unusual biphasic reaction consistent with complete re ... | 1987 | 3593351 |
| achromobacter xylosoxidans bacteremia. | 1987 | 3595393 | |
| the crystal structure of pseudoazurin from alcaligenes faecalis s-6 determined at 2.9 a resolution. | the three-dimensional structure of pseudoazurin, a single copper-containing protein from alcaligenes faecalis strain s-6, has been determined at 2.9 a resolution by x-ray crystallography. the sequences of two other pseudoazurins from pseudomonas am1 and achromobacter cycloclastes may also be accommodated in this structure. the structure, an eight-stranded beta-barrel, resembles closely those of plastocyanin and azurin. it possesses two extra alpha-helices at the c-terminus, whereas azurins have ... | 1987 | 3595868 |
| achromobacter xylosoxidans meningitis: case report and review of the literature. | 1987 | 3621776 | |
| identification of dimethyl disulfide-forming bacteria isolated from activated sludge. | twenty-four strains with high dimethyl disulfide (dmds)-forming ability were isolated from activated sludge and identified to the genus level. these bacteria were classified into four groups (a, b, c, and d) by the api zym system (api system s.a., montalieu, france). group a (three strains) was identified as genus lactobacillus by the api 20b system, by the method of cowan and steel, and by production of lactic acid as confirmed by gas-liquid chromatography. group b (eight strains) was identifie ... | 1987 | 3662505 |
| diversity of plasmids in achromobacter xylosoxidans isolates responsible for a seemingly common-source nosocomial outbreak. | achromobacter xylosoxidans, an uncommon yet highly resistant opportunistic pathogen, was isolated from nine hospitalized patients during an 8-month period. it had been isolated from only seven patients with either nonfatal infection or colonization from 1981 to 1984. from june 1985 to january 1986, a. xylosoxidans was isolated 18 times from seven different sites (sputum, 7 times; urine, 4 times; blood, 3 times; and lung, pleural fluid, wound tissue, and tracheal aspirate, 1 time each). four pati ... | 1987 | 3667915 |
| use of a chemiluminescence detector for quantitation of nitric oxide produced in assays of denitrifying enzymes. | we have developed a closed-flow system that continuously sweeps away gases evolved in enzyme assay mixtures into a commercially available oxides of nitrogen (nox) analyzer for the quantitation of any nitric oxide present in these gases. the system enabled us to study both the stoichiometry and the kinetics of no production by a copper-containing nitrite reductase (ec 1.7.99.3) purified from achromobacter cycloclastes iam 1013. in addition to its much greater sensitivity in comparison with standa ... | 1987 | 3674405 |
| properties and electron transfer specificity of copper proteins from the denitrifier "achromobacter cycloclastes". | a blue copper protein (mr 12,000) was purified from cells of "achromobacter cycloclastes" grown as a denitrifier. when reduced, the blue copper protein transferred electrons to the copper protein nitrite reductase purified from the same cells, whereas a variety of cytochromes from denitrifiers failed to do so. inclusion of a protease inhibitor, phenylmethylsulfonyl fluoride, in the buffers employed during preparation yielded purified blue copper protein with 18 more amino acid residues and two t ... | 1986 | 3700338 |
| complete amino acid sequence of nadh-cytochrome b5 reductase purified from human erythrocytes. | the complete amino acid sequence of soluble nadh-cytochrome b5 reductase purified from human erythrocytes was determined. the enzyme, which contained 8 methionine residues, was cleaved by cyanogen bromide. the resulting nine peptides were separated by gel filtration and purified further by high-performance liquid chromatography. the purified peptides were sequenced by automated edman degradation. three large cnbr peptides, residues 1-101, 109-151, and 169-231, were further fragmented with trypsi ... | 1986 | 3700359 |
| infections with achromobacter xylosoxidans. | 1986 | 3715491 | |
| [amino acid metabolism by thin layer chromatography for the differentiation of bordetella and alcaligenes species]. | the amino acid metabolism of bordetella and alcaligenes strains was investigated for differentiation purposes. all species investigated reacted differently with a number of amino acids. the genus bordetella could be differentiated from that of alcaligenes by its inability to metabolize l-lysine, l-arginine and d-histidine. the recently described species bordetella avium, as well as achromobacter xylosoxidans (yabuuchi), considered as alcaligenes denitrificans subsp. xylosoxidans, presented thems ... | 1986 | 3788350 |
| purification and partial amino acid sequence of human platelet membrane glycoproteins iib and iiia. | the glycoprotein (gp) iib-iiia complex was isolated from human platelet membranes by immunoaffinity chromatography using a monoclonal antibody specific for gp iib-iiia. gp iib and iiia were further separated in the presence of sodium dodecyl sulfate (sds) by gel filtration high-performance liquid chromatography (hplc). two cycles of this procedure yielded almost complete separation of homogeneous preparations of gp iib and iiia. each protein was then digested with lysyl endopeptidase (achromobac ... | 1987 | 3801670 |
| infections due to achromobacter xylosoxidans. case report and review of the literature. | achromobacter xylosoxidans is an uncommon nosocomial pathogen known to cause many serious infections. a 69-year-old woman with diabetes mellitus and chronic renal failure was admitted with pulmonary edema. the patient developed fever and pulmonary infiltrate with bilateral pleural effusions while she was on a respirator in the intensive care unit. culture of sputum, pleural fluid and blood grew a. xylosoxidans. bilateral chest tubes were inserted and the patient was treated for one month with pi ... | 1986 | 3818105 |
| antibiotic susceptibilities of achromobacter xylosoxidans. | 1985 | 3834609 | |
| complete amino acid sequence of bovine colostrum low-mr cysteine proteinase inhibitor. | the complete amino acid sequence of bovine colostrum cysteine proteinase inhibitor was determined by sequencing native inhibitor and peptides obtained by cyanogen bromide degradation, achromobacter lysylendopeptidase digestion and partial acid hydrolysis of reduced and s-carboxymethylated protein. achromobacter peptidase digestion was successfully used to isolate two disulfide-containing peptides. the inhibitor consists of 112 amino acids with an mr of 12787. two disulfide bonds were established ... | 1985 | 3891407 |
| exposed tyrosine residues of lambda cro repressor protein evidenced by nitration and photo cidnp experiments. | the tyrosine residues of lambda cro repressor were partially nitrated with tetranitromethane under mild conditions. after digestion by achromobacter protease i, the extent of nitration was determined by hplc and amino acid analysis. tyr 26 was most easily nitrated and tyr 51 followed it. tyr 10 was resistant to nitration. by comparison of the proton magnetic resonance spectrum of the partially nitrated cro protein with the above result, the aromatic proton resonances of the tyrosine side chains ... | 1985 | 3910646 |
| nitric oxide-dependent proton translocation in various denitrifiers. | respiration of no resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. paracoccus denitrificans, rhodopseudomonas sphaeroides subsp. denitrificans, "achromobacter cycloclastes," and rhizobium japonicum gave, respectively, h+/no ratios of 3.65, 4.96, 1.94, and 1.12. antimycin a completely inhibited no-dependent proton translocation in p. denitrificans and severely restricted translocation in the r. sphaeroides strain. proton uptake ... | 1985 | 3928599 |
| subtilosin a, a new antibiotic peptide produced by bacillus subtilis 168: isolation, structural analysis, and biogenesis. | subtilosin a, a new antibiotic produced by bacillus subtilis 168, was extracted from culture medium with n-butanol and purified to homogeneity by a combination of gel filtration and thin-layer chromatography. the yield was 5.5 mg from a liter of culture. it had bacteriocidal activity against some gram-positive bacteria. amino acid analysis and mass spectrometry showed that it was a peptide with a molecular weight of 3398.9, consisting of 32 usual amino acid and some non-amino acid residues. its ... | 1985 | 3936839 |
| occurrence and antimicrobial susceptibility of gram-negative nonfermentative bacilli in cystic fibrosis patients. | isolation of nonfermentative gram-negative bacilli (other than pseudomonas aeruginosa) from respiratory tract cultures of cystic fibrosis (cf) patients has increased in recent years. species recovered include pseudomonas cepacia, p. maltophilia, p. fluorescens/putida, p. alcaligenes, p. pseudoalcaligenes, p. stutzeri, acinetobacter spp., achromobacter xylosoxidans, flavobacterium spp., and cdc groups ive and ve. although colonization with most of these organisms is sporadic, p. cepacia (and to a ... | 1985 | 3979021 |
| [nature of factors stimulating cobalamin production by achromobacter cobalamini]. | the object of this work was to study the nature of factors contained in molasses and maize extract and stimulating cobalaminogenesis in achromobacter cobalamini. the activity of substrate fractions was analyzed to show that the stimulating substance was precipitated on the cation exchanger and eluted from it with hcl. the factor was found to be an organic nitrogen base readily soluble in water and ethanol but insoluble in ether, chloroform and methanol. it was stable upon heating in concentrated ... | 1985 | 4010546 |
| neonatal meningitis caused by achromobacter xylosoxidans. | the clinical and bacteriological findings in a case of neonatal meningitis caused by achromobacter xylosoxidans are presented. this appears to be only the second report of meningitis caused by this species. | 1985 | 4044804 |
| the effect of dl-lactate on regulation of porphyrin and heme biosynthesis in escherichia coli and achromobacter. | 1974 | 4137260 | |
| enzymatic production of urocanic acid by achromobacter liquidum. | to develop an efficient method for the production of urocanic acid, optimal conditions for the production of microbial l-histidine ammonia lyase and for the conversion of l-histidine to urocanic acid by this enzyme were studied. a number of microorganisms were screened to test their ability to form and accumulate urocanic acid from l-histidine. achromobacter liquidum was selected as the best organism. with this organism, enzyme activity as high as 2.0 units/ml could be produced by a shaking cult ... | 1974 | 4151117 |
| nitrite reductase from achromobacter fischeri: molecular weight and subunit structure. | 1974 | 4151478 | |
| [cytochrome absorption spectra of bacteria as aid for solving taxonomic problems. 2. the redox and carbon monoxide difference spectra of bacterial species of the families enterobacteriaceae, and pasteurellaceae, and of the genera aeromomas, xanthomonas, vibrio, acinetobacter, alcaligenes, and achromobacter (author's transl)]. | 1974 | 4152660 | |
| volatile compounds produced in sterile fish muscle (sebastes melanops) by pseudomonas putrefaciens, pseudomonas fluorescens, and an achromobacter species. | volatile compounds produced by pseudomonas putrefaciens, p. fluorescens, and an achromobacter species in sterile fish muscle (sebastes melanops) were identified by combined gas-liquid chromatography and mass spectrometry. compounds produced by p. putrefaciens included methyl mercaptan, dimethyl disulfide, dimethyl trisulfide, 3-methyl-1-butanol, and trimethylamine. with the exception of dimethyl trisulfide, the same compounds were produced by an achromobacter species. methyl mercaptan and dimeth ... | 1973 | 4199335 |
| oxidation of polychlorinated biphenyls by achromobacter pcb. | 1973 | 4201383 | |
| bacterial metabolism of hydroxyproline. ii. metabolism of l-hydroxyproline in achromobacter. | 1965 | 4221993 | |
| enzymatic oxidation of 14c-labelled betaine by the marine microbe achromobacter cholinophagum. | 1968 | 4296155 | |
| udpacetylglucosamine dehydrogenase from achromobacter georgiopolitanum. | 1969 | 4312076 | |
| action of bacterial growth on the sarcoplasmic and urea-soluble proteins from muscle. i. effects of clostridium perfringens, salmonella enteritidis, achromobacter liquefaciens, streptococcus faecalis, and kurthia zopfi. | comparisons of the starch-gel patterns of uninoculated aseptic control samples from rabbit and pig muscle with similar samples inoculated and incubated with clostridium perfringens, salmonella enteritidis, achromobacter liquefaciens, and kurthia zopfii were made. results indicated that c. perfringens caused extensive alteration in the proteins or enzymes, or both, of the sarcoplasmic fraction of porcine muscle, whereas s. enteritidis and s. faecalis caused complete breakdown of only myoglobin. n ... | 1970 | 4318570 |
| halotolerant collagenolytic activity of achromobacter iophagus. | 1973 | 4352949 | |
| exocellular pyruvate-containing galactoglucan of achromobacter spp. | 1974 | 4365641 | |
| [influence of adenosine (ad) and 3-amino-1,2,4-triazole (at) on growth and porphyrin synthesis from delta-aminolevulinic acid (ala) in achromobacter metalcaligenes]. | 1967 | 4384845 | |
| purification and properties of a disulfide reductase obtained from achromobacter starkeyi. | 1968 | 4385907 | |
| purification, characterization and properties of nitrite reductase of achromobacter fischeri. | 1972 | 4401695 | |
| microbial metabolism of pyridinium compounds. metabolism of 4-carboxy-1-methylpyridinium chloride, a photolytic product of paraquat. | 1. a bacterium, achromobacter d, isolated from garden soil by elective culture, utilized n-methylisonicotinic acid (4-carboxy-1-methylpyridinium chloride) as sole carbon source. 2. extracts of n-methylisonicotinate-grown cells oxidized this substrate only after supplementation with a source of nicotinamide nucleotides and then consumed 1 mol of o(2) and released 1 mol of co(2)/mol of n-methylisonicotinate supplied. 3. the n-methyl group of the substrate was released as methylamine whereas the fi ... | 1972 | 4404506 |
| metabolism of nitrate in achromobacter fischeri. | 1973 | 4405508 | |
| studies on the isocitrat-lyase in achromobacter. | 1974 | 4407683 | |
| taxonomic significance of phenethyl alcohol production by achromobacter isolates from fishery sources. | the formation of phenethyl alcohol from l-phenylalanine and ethanol by achromobacter isolates of fishery origin was found to be taxonomically significant for such organisms. phenylpyruvate, the direct oxidative deamination product of l-phenylalanine, was found to serve as an intermediate precursor to phenethyl alcohol formation. among ten acinetobacter isolates examined, none produced phenethyl alcohol. among nine moraxella isolates examined, one produced phenethyl alcohol. | 1974 | 4422523 |
| continuous production of urocanic acid by immobilized achromobacter liquidum cells. | 1974 | 4441634 | |
| [synthesis of group b vitamins by achromobacter cobalamini sp. nov]. | 1974 | 4444553 | |
| microbial metabolism of the pyridine ring. metabolism of 2- and 3-hydroxypyridines by the maleamate pathway in achromobacter sp. | 1. washed suspensions of two achromobacter species (g2 and 2l), capable of growth upon 2- and 3-hydroxypyridine respectively as sources of c and n, rapidly oxidized their growth substrate pyridine-2,5-diol (2,5-dihydroxypyridine) and the putative ring-cleavage product maleamate without a lag. suspensions derived from fumarate plus (nh(4))(2)so(4) cultures were unable to do so. 2. extracts of both bacteria oxidized pyridine-2,5-diol with the stoicheiometry of an oxygenase forming 1mol of nh(3)/mo ... | 1974 | 4455192 |
| reactions involved in bioluminescence systems of limpet (latia neritoides) and luminous bacteria. | luminescence in latia involves a specific flavoprotein enzyme ("luciferase"), which has a tightly bound flavin group constituting the light-emitter. the overall reaction includes oxidation of a specific substrate ("luciferin," an enol formate derivative of an aliphatic aldehyde), by 2 o(2) molecules, in the presence of a "purple protein" cofactor, yielding a ketone, hcooh, co(2), and light. in achromobacter, a required aliphatic aldehyde, which is functionally equivalent to latia luciferin, is o ... | 1972 | 4506078 |
| [description of 2 new species of bactera isolated from pozol: agrobacterium azotophilum and achromobacter pozolis]. | 1972 | 4509280 | |
| the aldehyde content of luminous bacteria and of an "aldehydeless" dark mutant. | fatty aldehydes, present in the luminescent cells of photobacterium phosphoreum and achromobacter fischeri, and to a very slight extent in the cells of a visually dark, "aldehydeless" mutant of the latter species, were extracted, purified, and oxidized to the corresponding acids. the acids were analyzed by mass spectrometry. the results, in conjunction with various other lines of evidence, indicate that saturated fatty aldehydes, comprising mostly dodecanal, tetradecanal, and hexadecanal, functi ... | 1974 | 4531008 |
| oxygen requirements of strains of pseudomonas and achromobacter. | 1972 | 4560487 |