Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acquisition of plasmin activity by fusobacterium nucleatum subsp. nucleatum and potential contribution to tissue destruction during periodontitis. | fusobacterium nucleatum subsp. nucleatum has been associated with a variety of oral and nonoral infections such as periodontitis, pericarditis, bone infections, and brain abscesses. several studies have shown the role of plasmin, a plasma serine protease, in increasing the invasive capacity of microorganisms. in this study, we investigated the binding of human plasminogen to f. nucleatum subsp. nucleatum, and its subsequent activation into plasmin. plasminogen-binding activity of bacterial cells ... | 1999 | 10569761 |
| influence of environmental conditions on hydrogen peroxide formation by streptococcus gordonii. | hydrogen peroxide generated by viridans group streptococci has an antagonistic effect on many bacterial species, including a number of pathogens, in the oral environment. this study examines the influence of a variety of environmental conditions on rates of hydrogen peroxide synthesis by streptococcus gordonii. hydrogen peroxide was synthesized at every concentration of glucose and sucrose tested from 10 microm to 1 m, with the highest rates occurring at 0.1 mm sucrose and 1 mm glucose. s. gordo ... | 1999 | 10569775 |
| pre- and post-implantation microbiota of the tongue, teeth, and newly placed implants. | this investigation sought intra-oral sources of species colonizing dental implants. | 1999 | 10599911 |
| clinically applicable multiplex pcr for four middle ear pathogens. | the multiplex pcr method for the detection of alloiococcus otitidis, haemophilus influenzae, moraxella catarrhalis, and streptococcus pneumoniae (p. h. hendolin, a. markkanen, j. ylikoski, and j. j. wahlfors, j. clin. microbiol. 35:2854-2858, 1997) in middle ear effusions (mees) was modified to be better suited for clinical use. to detect false-negative results, an internal amplification was added to the reaction, and to prevent carryover contamination, the dutp-uracil-n-glycosidase system was i ... | 2000 | 10618075 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| periodontal findings and systemic antibody responses to oral microorganisms in behçet's disease. | behçet's disease is a multisystem disorder of unknown etiology, affecting predominantly the oral mucosa, skin, and eyes. recurrent and painful episodes of oral ulcerations interfere with regular oral hygiene leading to rapid bacterial plaque accumulation. the aims of this study were to evaluate the periodontal status of patients with behçet's disease and determine serum antibody responses to selected oral microorganisms, including major periodontopathogens in these patients. | 1999 | 10632520 |
| s-layer proteins. | 2000 | 10648507 | |
| evaluation of the oxyrase oxyplate anaerobe incubation system. | the oxyrase oxyplate anaerobe incubation system was evaluated for its ability to support the growth of clinically significant anaerobic bacteria previously identified by the anaerobe reference laboratory at the centers for disease control and prevention. the results were compared with those obtained with conventional anaerobe blood agar plates incubated in an anaerobe chamber. we tested 251 anaerobic bacterial strains. plates were read at 24, 48, and 72 h; growth was scored by a numerical coding ... | 2000 | 10655335 |
| use of defined mutants to assess the role of the campylobacter rectus s-layer in bacterium-epithelial cell interactions. | campylobacter rectus is a periodontal pathogen with a 150-kda protein on its cell surface. this protein forms a paracrystalline lattice, called the s-layer, surrounding the outer membrane of this gram-negative bacterium. to initiate a genetic analysis of the possible role of the s-layer in the initial interaction of c. rectus with host epithelial cells, c. rectus strains lacking the s-layer protein gene (crsa) were constructed by allelic exchange mutagenesis. surprisingly, the lack of the s-laye ... | 2000 | 10678961 |
| lack of antimicrobial effect on periodontopathic bacteria by ultrasonic and sonic scalers in vitro. | the purpose of this study was to assess the antimicrobial effects of a sonic and ultrasonic scaler generally used for subgingival scaling on gram-negative and gram-positive periodontopathic bacteria. | 2000 | 10703657 |
| induction of apoptotic cell death in peripheral blood mononuclear and polymorphonuclear cells by an oral bacterium, fusobacterium nucleatum. | it is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of competent immune effector cells. in this paper we present evidence for the immunosuppressive role of fusobacterium nucleatum, a gram-negative oral bacterium which plays an important role in the generation of periodontal disease. our studies indicate that the immunosuppressive role of f. nucleatum is lar ... | 2000 | 10722579 |
| campylobacter species in health, gingivitis, and periodontitis. | at least seven campylobacter species have been identified from subgingival sites. campylobacter rectus has been implicated as a periodontal pathogen; however, association with periodontal infections of other campylobacter species, especially the newly described campylobacter showae, is unclear. this study examined which campylobacter species were associated with periodontal health and disease. subgingival campylobacter species from initial and established periodontitis were compared with species ... | 2000 | 10728981 |
| genetic structure of population of bacillus cereus and b. thuringiensis isolates associated with periodontitis and other human infections. | the genetic diversity and relationships among 35 bacillus cereus and bacillus thuringiensis isolates recovered from marginal and apical periodontitis in humans and from various other human infections were investigated using multilocus enzyme electrophoresis. the strains were isolated in norway, except for three strains isolated from periodontitis patients in brazil. the genetic diversity of these strains was compared to that of 30 isolates from dairies in norway and finland. allelic variation in ... | 2000 | 10747152 |
| the prevalence of pathogenic periodontal microflora in healthy young adult smokers. | smoking is a major risk factor in periodontitis, although the mechanisms of its effects are not well understood. the overall goal of this clinical study was to determine if smoking enhances the colonization of the oral cavity by pathogenic bacteria in a periodontitis-free population. the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsythus, and treponema denti ... | 2000 | 10807119 |
| an ompa-like protein from acinetobacter spp. stimulates gastrin and interleukin-8 promoters. | bacterial overgrowth in the stomach may occur under conditions of diminished or absent acid secretion. under these conditions, secretion of the hormone gastrin is elevated. alternatively, bacterial factors may directly stimulate gastrin. consistent with this hypothesis, we found that mice colonized for 2 months with a mixed bacterial culture of opportunistic pathogens showed an increase in serum gastrin. to examine regulation of gene expression by bacterial proteins, stable transformants of ags ... | 2000 | 10816525 |
| periodontopathic bacteria in english hiv-seropositive persons. | selected periodontopathic bacteria were sought in 20 hiv-infected english patients and eight noninfected control subjects with similar periodontal status, using highly specific dna probes. actinobacillus actinomycetemcomitans (a. a), porphyromonas gingivalis, campylobacter rectus, prevotella intermedia, and fusobacterium nucleatum were more frequently detected and were found at higher levels in hiv-infected individuals than in controls. significantly increased levels of treponema denticola but, ... | 1999 | 10842858 |
| identification of ragab as a temperature-regulated operon of porphyromonas gingivalis w50 using differential display of randomly primed rna. | porphyromonas gingivalis is a gram-negative, black-pigmented anaerobe that has been associated with advanced periodontal disease. the genome of p. gingivalis has the potential to produce a number of virulence determinants including proteases, hemagglutinins, hemolysin, invasion-associated proteins, and products of the pathogenicity island ragab; however, little is known about how their expression is controlled. periodontal pockets experience a higher temperature during inflammation, and this ele ... | 2000 | 10858216 |
| human serum antibody responses to oral microorganisms. iv. correlation with homologous infection. | recent microbiological studies of periodontal disease in humans have supported the concept of a specific bacterial etiology. while individual agents have not been unequivocally identified, numerous gram-negative members of the subgingival microflora have been implicated. in addition, elevations in systemic antibody responses have been consistent with certain oral microorganisms being involved in an infectious process associated with the disease. this report delineates the relationship between el ... | 1987 | 10870468 |
| features of severe periodontal disease in a teenager with chédiak-higashi syndrome. | chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis. | 2000 | 10872965 |
| "checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study. | we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status. | 2000 | 10914791 |
| rapid detection of campylobacter jejuni in stool specimens by an enzyme immunoassay and surveillance for campylobacter upsaliensis in the greater salt lake city area. | the alexon-trend, inc. (ramsey, minn.), prospect campylobacter microplate assay was compared with culture on a campy-cva plate (remel, lenexa, kans.) and blood-free campylobacter agar with cefoperazone (20 microg/ml), amphotericin b (10 microg/ml), and teicoplanin (4 microg/ml) (cat medium; oxoid limited, hampshire, england) with 631 patient stool samples. the cat medium was used to isolate campylobacter upsaliensis. the enzyme immunoassay (eia) had a sensitivity and a specificity of 89 and 99%, ... | 2000 | 10921981 |
| fusobacterium nucleatum involvement in adult periodontitis and possible modification of strain classification. | this investigation was designed to evaluate the involvement of fusobacterium nucleatum clinical strains in adult periodontitis by subspecies and expression of hemagglutination activity. | 2000 | 10960022 |
| identification of periodontal disease-associated bacteria in the "plaque-free zone". | subgingival plaque bacteria live within a biofilm covered with glycocalyx, and little is known of the bacterial species associated with biofilm formation at the bottom of human periodontal pockets, the so-called "plaque-free zone"(pfz). | 2000 | 10972648 |
| efficient isolation of campylobacteria from stools. | 2000 | 10979752 | |
| prevalence of periodontal pathogens in localized and generalized forms of early-onset periodontitis. | the primary objectives of this study were to investigate the prevalence of 8 putative periodontal pathogens in subjects with early-onset periodontitis (eop) and to evaluate the microbial differences between localized and generalized forms of this periodontal disease condition. thirty-one females and 11 males with a mean age of 30.3 (s.d. 4.0) years were examined. seventeen subjects had generalized (geop) and 25 had localized early-onset periodontitis (leop). subgingival plaque samples were assay ... | 2000 | 10983884 |
| b-cell deficiency predisposes mice to disseminating anaerobic infections: protection by passive antibody transfer. | we have previously demonstrated that a high proportion of rag-2 scid knockout mice, which lack t and b cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. in the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. for this purpose, various genetically engineered immunodeficient mice were employed, ... | 2000 | 10992465 |
| comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16s rdna libraries. | the oral bacterial flora in the saliva from two patients with periodontitis and from a periodontally healthy subject were compared using a sequence analysis of 16s rdna libraries without cultivation. 16s rdnas were amplified from salivary dna by pcr and cloned. randomly selected clones were partially sequenced. on the basis of sequence similarities, the clones were classified into several clusters corresponding to the major phylum of the domain bacteria. the major phylum in the libraries was the ... | 2000 | 11021394 |
| microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients. | polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities. | 2000 | 11073323 |
| photoactive porphyrin derivative with broad-spectrum activity against oral pathogens in vitro. | photodynamic therapy (pdt) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. pdt is a potential adjunct to scaling and root planing in the treatment of periodontal disease. however, the effectiveness of porphyrin derivatives against microorganisms has been limited because some gram-negative bacteria are refractory to photodynamic treatment with these agents. we have designed a porphyrin ... | 2000 | 11083641 |
| [clinical use of tetracyclines in the treatment of periodontal diseases]. | introduction: there are a number of chemically different tetracycline homologues. the older group of tetracyclines, which was introduced in the 1950-60s, includes tetracycline, oxytetracycline, chlortetracycline and demeclocycline. the newer group of tetracyclines includes doxycycline, methacycline and minocycline. pharmacokinetics: elevated concentration of tetracycline in gingival fluid with respect to blood levels was an unexpected phenomenon. patients given 250 mg every 6 hours had average c ... | 2000 | 11089368 |
| porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets. | recent evidence has established an association between chronic periodontitis and cardiovascular disease. periodontitis is a chronic inflammatory disease caused by a small group of gram-negative bacteria, of which porphyromonas gingivalis is considered an important causative agent. it has been proposed that dental plaque bacteria and their products can disseminate into the bloodstream from the site of infection and promote thromboembolic events associated with atherosclerosis and myocardial infar ... | 2000 | 11154438 |
| improved, low-cost selective culture medium for actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans is considered to be one of the major oral putative pathogens, especially in cases of juvenile periodontitis. this microorganism requires nutritionally complex media for growth, and therefore the media for its primary isolation usually include blood agar or serum in their base. in this study we present a new medium, dentaid-1, which improves the detection of a. actinomycetemcomitans in periodontal samples. in its composition, blood and serum have been omitted, ... | 2001 | 11158098 |
| detection of treponema denticola in atherosclerotic lesions. | we examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16s rrna locus by pcr. treponema denticola sequence of the 16s rrna locus was found in 6 out of 26 dna samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. utilizing immunofluorescence microscopy, we observed aggregated antigenic particles re ... | 2001 | 11230436 |
| specific detection and prevalence of helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16s ribosomal dna sequencing. | gastric infection with helicobacter heilmannii (previously known as gastrospirillum hominis) is invariably linked with the presence of chronic gastritis and the risk of developing low-grade mucosa-associated lymphoid tissue lymphoma in humans. in contrast to helicobacter pylori, various h. heilmannii species colonize the stomachs of domestic animals, which might be a reservoir for transmission to humans (zoonosis). to identify the number and prevalence of different h. heilmanni types in humans, ... | 2001 | 11283079 |
| shared antigenicity between helicobacter pylori and periodontopathic campylobacter rectus strains. | periodontopathic campylobacter rectus strains possess 41- and 68-kda proteinaceous antigens which share antigenicity with antigens of helicobacter pylori strains. h. pylori strains have a 54-kda antigen which reacts with c. rectus strains. we found that the salivary iga levels against h. pylori were correlated with those against c. rectus. these cross-reactive antigens of c. rectus may affect the serological diagnosis of h. pylori infections, especially when saliva is used. it is possible that t ... | 2001 | 11287141 |
| periodontal pathogens produce quorum sensing signal molecules. | different species of bacteria important in the composition of dental plaque were tested for production of extracellular autoinducer-like activities that stimulate the expression of the luminescence genes in vibrio harveyi. several strains of prevotella intermedia, fusobacterium nucleatum, and porphyromonas gingivalis were found to produce such activities. interestingly, these bacteria belong to the same phylogenetic group, and they are periodontal pathogens important in the development of period ... | 2001 | 11292769 |
| comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental regions. | it has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ... | 2000 | 11307190 |
| relationship of periodontopathic bacteria with early-onset periodontitis in down's syndrome. | down's syndrome (ds) patients often develop severe early-onset marginal periodontitis in early adulthood; however, there is little information available on the microbiology of ds periodontitis. | 2001 | 11327065 |
| antimicrobial activity of silver nitrate against periodontal pathogens. | metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ... | 2001 | 11327077 |
| periodontal infections and pre-term birth: early findings from a cohort of young minority women in new york. | the aim of this report is to provide early data from an ongoing study examining (i) the relationship between periodontal infections and pre-term low birth weight (plbw) in a cohort of young, minority, pregnant and post-partum women; and (ii) the effect of periodontal interventions on pregnancy outcome. during the first 2 yr of the study, 213 women were enrolled and examined clinically for dental plaque, calculus, bleeding on probing, and probing depth. birth outcome data were available for 164 w ... | 2001 | 11330932 |
| pcr detection of 5 putative periodontal pathogens in dental plaque samples from children 2 to 12 years of age. | the purpose of this study was to detect the presence of prevotella intermedia, p. nigrescens, bacteroides forsythus, treponema denticola, and campylobacter rectus in plaque samples from 119 children, collected from their toothbrushes using a polymerase chain reaction (pcr). | 2001 | 11350526 |
| bacterial diversity in human subgingival plaque. | the purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16s rrna sequences for all cultivable and not-yet-cultivated species of human oral bacteria. subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16s ribos ... | 2001 | 11371542 |
| smoking affects the subgingival microflora in periodontitis. | tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers. | 2001 | 11394403 |
| untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of periodontitis. | in an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up. | 2001 | 11422582 |
| c-telopeptide pyridinoline cross-links (ictp) and periodontal pathogens associated with endosseous oral implants. | detection of periodontal or peri-implant sites exhibiting progressing disease or those at risk of deterioration has proven difficult. pyridinoline cross-linked carboxyterminal telopeptide of type i collagen (ictp), a marker specific for bone degradation found in gingival crevicular fluid (gcf), has been associated with both bone and attachment loss in periodontitis and may be useful for predicting disease activity. the aim of this cross-sectional study was to examine the relationship between ict ... | 1998 | 11429938 |
| arginine-specific protease from porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion. | periodontitis is a chronic inflammatory disease affecting oral tissues. oral epithelial cells represent the primary barrier against bacteria causing the disease. we examined the responses of such cells to an arginine-specific cysteine proteinase (rgpb) produced by a causative agent of periodontal disease, porphyromonas gingivalis. this protease caused an intracellular calcium transient in an oral epithelial cell line (kb), which was dependent on its enzymatic activity. since protease-activated r ... | 2001 | 11447194 |
| inhibition of interleukin-1beta production and gene expression in human gingival fibroblasts by low-energy laser irradiation. | human gingival fibroblast (hgf) cells reside in gingival tissues which are challenged frequently by oral bacteria. lipopolysaccharide (lps) from periodontal pathogens can penetrate gingival tissues and stimulate the production of interleukin-1beta (il-1beta), which has been implicated in inflammation and bone resorption. the anti-inflammatory effects of low-energy laser irradiation have been reported, but the mechanisms of this biostimulatory effect have not been fully elucidated. primary cultur ... | 2001 | 11482821 |
| treatment of peri-implantitis by local delivery of tetracycline. clinical, microbiological and radiological results. | the purpose of this study was to investigate the clinical, microbiological and radiological effects of peri-implantitis therapy by local delivery of tetracycline. in 25 partially edentulous patients, 30 implants with radiographic evidence of circumferential bone loss, and peri-implant probing depths > or =5 mm were treated with polymeric tetracycline hcl-containing fibers. clinical and microbial parameters were recorded at baseline, and 1, 3, 6, and 12 months (m) after treatment. standardized ra ... | 2001 | 11488856 |
| clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene. | this is the first known case report that used a polymerase chain reaction (pcr)-based method to help identify the oral microflora in patients with chronic neutropenia. in this study, we report clinical periodontal findings and microflora profiles of 2 children, 1 with severe congenital neutropenia (scn, kostmann type) and 1 with cyclic neutropenia (cn). | 2001 | 11495144 |
| molecular identification of campylobacter concisus. | a 1.6-kb dna fragment isolated from a campylobacter concisus genomic library gave c. concisus-specific restriction fragment length patterns when it was used as a probe in hybridization studies. all of the strains tested, including type strains and clinical isolates, contained a 0.5-kb hindiii fragment that hybridized to the probe. dna sequencing of the 1.6-kb fragment identified three open reading frames (orfs). one of the orfs encodes the carboxy terminus of gyrb, and the translational products ... | 2001 | 11574591 |
| periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. | periodontal disease is perhaps the most common chronic infection in adults. evidence has been accumulating for the past 30 years which indicates that almost all forms of periodontal disease are chronic but specific bacterial infections due to the overgrowth in the dental plaque of a finite number of mostly anaerobic species such as porphyromonas gingivalis, bacteroides forsythus, and treponema denticola. the success of traditional debridement procedures and/or antimicrobial agents in improving p ... | 2001 | 11585783 |
| comparative in vitro activity of ertapenem and 11 other antimicrobial agents against aerobic and anaerobic pathogens isolated from skin and soft tissue animal and human bite wound infections. | we studied the comparative in vitro activity of ertapenem, a new carbapenem, against 240 aerobic and 180 anaerobic recent clinical bite isolates using an agar dilution method and an inoculum of 10(4) cfu/spot for aerobes and 10(5) cfu/spot for anaerobes. ertapenem inhibited 410/420 (98%) of the isolates tested at < or = 4 mg/l with only 4/5 campylobacter gracilis and 1/3 campylobacter rectus strains requiring . or = 16 mg/l for inhibition. ertapenem was only moderately active (mic 8 mg/l) agains ... | 2001 | 11679553 |
| efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters. | the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition. | 2001 | 11686813 |
| absence of a specific subgingival microflora in adults with down's syndrome. | periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed. | 2001 | 11686820 |
| efficient isolation of campylobacter upsaliensis from stools. | 2001 | 11712518 | |
| association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. | the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions. | 2001 | 11737506 |
| associations between serum antibody levels to periodontal pathogens and early-onset periodontitis. | the role of antibodies to periodontal microorganisms in the development of periodontal tissue destruction is still unclear. the aim of this study was to investigate the association between serum levels of igg, iga, and igm antibodies to 6 periodontal microorganisms and clinical subtypes of varying severity of early-onset periodontitis (eop) in young african american adults. | 2001 | 11759856 |
| succinate:quinone oxidoreductases from epsilon-proteobacteria. | the epsilon-proteobacteria form a subdivision of the proteobacteria including the genera wolinella, campylobacter, helicobacter, sulfurospirillum, arcobacter and dehalospirillum. the majority of these bacteria are oxidase-positive microaerophiles indicating an electron transport chain with molecular oxygen as terminal electron acceptor. however, numerous members of the epsilon-proteobacteria also grow in the absence of oxygen. the common presence of menaquinone and fumarate reduction activity su ... | 2002 | 11803019 |
| [a comparative study of various evaluation methods of the antibiotic sensitivity of strict anaerobic bacteria of the subgingival flora]. | the study on the sensitiveness of slow-growing anaerobes bacteria to antibiotics is delicate when you consider the technical motives that make it difficult to transpose the standard methods frequently used in microbiological laboratories. the three main methods used to determine susceptibility to antibiotics are: disk-diffusion test, antibiotics containing microdilution plates and atb ana (biomérieux). the aim of this study is to compare the effectiveness of each of these methods on severe anaer ... | 2001 | 11808376 |
| periodontopathic bacterial infection in childhood. | little information is available on periodontopathic bacterial infection in childhood. we assessed the prevalence by age of 10 putative periodontopathic microorganisms in periodontally healthy children using a polymerase chain reaction (pcr) assay. | 2002 | 11846195 |
| antigenic properties of the groel-like protein of campylobacter rectus. | the purpose of this study was to clarify the antigenic properties of the groel-like protein of campylobacter rectus using a specific polyclonal antibody directed to the purified 64-kda groel-like protein (pab-crgroel), a polyclonal antibody directed to the actinobacillus actinomycetemcomitans groel-like protein (pab-aagroel) and a monoclonal antibody against the recombinant human hsp60 (mab-huhsp60). in sds-page/western immunoblotting analysis, mab-huhsp60, pab-crgroel and pab-aagroel were found ... | 2002 | 11860551 |
| a type 2 response in lipopolysaccharide (lps)-stimulated whole blood cell cultures from periodontitis patients. | it is acknowledged that periodontitis results from the interaction of the host immune response with bacteria accumulating on the tooth surfaces. although bacteria are essential, they are insufficient to cause the disease. despite this knowledge it remains unclear why certain individuals are more susceptible to periodontitis than others. therefore the present study investigated whether differences exist in the actual immune response between periodontitis patients and controls after stimulation of ... | 2002 | 11876764 |
| maternal periodontitis and prematurity. part ii: maternal infection and fetal exposure. | clinical data from the first 812 deliveries from a cohort study of pregnant mothers entitled oral conditions and pregnancy (ocap) demonstrate that both antepartum maternal periodontal disease and incidence/progression of periodontal disease are associated with preterm birth and growth restriction after adjusting for traditional obstetric risk factors. in the current study we present measures of maternal periodontal infection using whole chromosomal dna probes to identify 15 periodontal organisms ... | 2001 | 11887461 |
| clinical and microbiological studies of periodontal disease in sjögren syndrome patients. | little is known about the periodontal status of patients with sjögren's syndrome (ss), a chronic inflammatory autoimmune disease characterized by xerophthalmia and xerostomia. the aim of the present study was to evaluate whether the periodontal status of ss patients, in terms of clinical and microbiological parameters, differs from systemically healthy age- and gender-matched controls. | 2002 | 11908470 |
| biofilms: survival mechanisms of clinically relevant microorganisms. | though biofilms were first described by antonie van leeuwenhoek, the theory describing the biofilm process was not developed until 1978. we now understand that biofilms are universal, occurring in aquatic and industrial water systems as well as a large number of environments and medical devices relevant for public health. using tools such as the scanning electron microscope and, more recently, the confocal laser scanning microscope, biofilm researchers now understand that biofilms are not unstru ... | 2002 | 11932229 |
| long-term follow-up of osseointegrated titanium implants using clinical, radiographic and microbiological parameters. | the aim of this study was to longitudinally follow up osseointegrated titanium implants in partially dentate patients by clinical, radiographic and microbiological parameters in order to evaluate possible changes in the peri-implant health over time. fifteen individuals treated with titanium implants, ad modum brånemark, and followed for ten years were included in the study. before implant placement ten years previously, the individuals had been treated for advanced periodontal disease and there ... | 2002 | 11952732 |
| accumulation of human heat shock protein 60-reactive t cells in the gingival tissues of periodontitis patients. | heat shock protein 60s (hsp60) are remarkably immunogenic, and both t-cell and antibody responses to hsp60 have been reported in various inflammatory conditions. to clarify the role of hsp60 in t-cell responses in periodontitis, we examined the proliferative response of peripheral blood mononuclear cells (pbmc), as well as the cytokine profile and t-cell clonality, for periodontitis patients and controls following stimulation with recombinant human hsp60 and porphyromonas gingivalis groel. to co ... | 2002 | 11953387 |
| [identification of periodontopathic bacteria dna from periodontitis-involved gingival tissue using a pcr method]. | the purpose of this study was to determine the presence of periodontopathic bacteria dna in the granulation tissue of periodontally involved gingival tissue. forty periodontitis patients were examined. subgingival plaque and saliva samples were collected from the patients before surgery. then, granulation tissue was also collected during surgery. another 20 patients, who had more than 4 mm in probing pocket depth, were also examined as a control. they received only initial treatment without peri ... | 2002 | 11968834 |
| effect of fusobacterium nucleatum on the t and b cell responses to porphyromonas gingivalis in a mouse model. | t cell cytokine profiles and specific serum antibody levels in five groups of balb/c mice immunized with saline alone, viable fusobacterium nucleatum atcc 25586, viable porphyromonas gingivalis atcc 33277, f. nucleatum followed by p. gingivalis and p. gingivalis followed by f. nucleatum were determined. splenic cd4 and cd8 cells were examined for intracytoplasmic interleukin (il)-4, interferon (ifn)-gamma and il-10 by dual colour flow cytometry and the levels of serum anti-f. nucleatum and anti- ... | 2002 | 11985513 |
| serum igg antibody response to periodontal pathogens in minority populations: relationship to periodontal disease status and progression. | differences in periodontal disease prevalence, severity, subgingival microflora and host immune response have been reported for various ethnic/racial groups, which implies that risk factors for destructive periodontal disease progression may also vary in these populations. as it is possible that these differences may be due to confounding variables other than ethnicity/race, we have measured serum igg antibody response to six periodontal pathogens, and compared these data with microbiological, c ... | 2002 | 12009183 |
| parotid salivary s-iga antibodies during experimental gingivitis in smokers and non-smokers. | persons who smoke display a less pronounced increase of gingival bleeding in the experimental gingivitis model as compared with non-smokers. the aim of the present study was to investigate whether this could partly be explained by differences in levels of parotid total secretory iga (s-iga) or parotid s-iga reactive with selected oral microorganisms. parotid saliva samples were obtained from 11 smoking and 14 non-smoking volunteers, at baseline, after 5 and 14 days of full mouth experimental gin ... | 2002 | 12009188 |
| activities of abt-773 against microaerophilic and fastidious organisms. | abt-773 was tested against 317 fastidious isolates; it inhibited 99% of organisms at a concentration of 4.0 microg/ml. with ampicillin-sulbactam and levofloxacin, only 2 and 6% of these strains, respectively, were resistant at the breakpoint. with clindamycin, penicillin g, and metronidazole, 22, 26, and 58% of the strains, respectively, were resistant. | 2002 | 12121965 |
| the effect of apically repositioned flap surgery on clinical parameters and the composition of the subgingival microbiota: 12-month data. | the purpose of this investigation was to examine the clinical and microbiologic effects of apically repositioned flap surgery. eighteen patients with chronic periodontitis received initial preparation (ip) including scaling and root planing, followed at 3 months by apically repositioned flap surgery at sites with pocket depth > 4 mm. subjects were monitored clinically and microbiologically at baseline, 3 months after ip, and at 3, 6, 9, and 12 months postsurgery. clinical assessments of plaque a ... | 2002 | 12186343 |
| microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders. | the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ... | 2002 | 12200976 |
| initial effect of controlled release chlorhexidine on subgingival microorganisms. | little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone. | 2002 | 12366861 |
| in vitro activities of faropenem against 579 strains of anaerobic bacteria. | the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ... | 2002 | 12384389 |
| antibacterial activity of moxifloxacin against periodontal anaerobic pathogens involved in systemic infections. | the in vitro activity of moxifloxacin was compared with that of penicillin g, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. mic(50)/mic(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for porphyromonas gingivalis (35), for prevotella spp. (28) and actinomyces spp. (35), 0.12/0.25 mg/l for fusobacterium nucleatum (20) and 0.06/0.12 mg/l for peptostreptococcus spp. (30). the minimum inhibitory ... | 2002 | 12458140 |
| correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese. | the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ... | 2002 | 12483235 |
| similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. | this study evaluated the similarity between the oral microbiota of young children and that of their adult caregivers. oral samples from children (174 dentate and 18 pre-dentate) aged 6-36 months and their caregivers in saipan were assayed using a dna probe assay. many species including streptococcus mutans, streptococcus sobrinus, actinomyces species, campylobacter rectus, fusobacterium nucleatum, prevotella intermedia, and porphyromonas gingivalis were detected in dentate and pre-dentate childr ... | 2002 | 12485330 |
| evaluation of 11 pcr assays for species-level identification of campylobacter jejuni and campylobacter coli. | we examined the sensitivity and specificity of 11 pcr assays described for the species identification of campylobacter jejuni and campylobacter coli by using 111 type, reference, and field strains of c. jejuni, c. coli, and campylobacter lari. for six assays, an additional 21 type strains representing related campylobacter, arcobacter, and helicobacter species were also included. pcr tests were initially established in the laboratory by optimizing conditions with respect to five type and referen ... | 2003 | 12517869 |
| igg subclass specific antibody response to periodontopathic organisms in hiv-positive patients. | we previously reported an increased rate of progression of periodontal disease over an 18-month period in human immunodeficiency virus (hiv)-positive subjects compared to controls. the mechanism for disease progression and rapid tissue loss was unknown. data on the microbiological studies failed to show any significant difference in the microbial characteristics of the periodontal lesions in hiv-positive patients compared to hiv-negative controls. immunological analysis had identified neutrophil ... | 2002 | 12546094 |
| determination of eight selected periodontal pathogens in the subgingival plaque of maxillary first molars in japanese school children aged 8-11 years. | to prevent the onset or progression of periodontitis, we must understand when periodontal pathogens are first harbored and how they develop the biofilm that causes periodontal disease. the purpose of this study was to determine the relationship between clinical status and selected periodontal pathogens in subgingival plaque in school children. this study was conducted with 95 school children, 8-11 years old. the presence and distribution of eight selected periodontal pathogens sampled from the m ... | 2003 | 12558934 |
| detection of campylobacter rectus in periodontitis sites by monoclonal antibodies. | campylobacter rectus, a gram-negative, microaerophilic, and motile bacterium, has been proposed to play a pathogenic role in human periodontitis. surface components, such as the flagellum, surface layer (s-layer), and cytotoxin, have been reported as possible virulence factors of the microorganism. in the present study, monoclonal antibodies against surface components of this bacterium were produced to detect and investigate the pathogenic potential of c. rectus in periodontitis. two monoclonal ... | 2003 | 12558939 |
| ecological and immunopathological implications of oral bacteria in helicobacter pylori-infected disease. | increasing evidence has linked colonization by helicobacter pylori with the development of gastritis and peptic ulcer disease. h. pylori resides primarily in the gastric mucosa without invading the gastric epithelium, causing persistent mild gastric inflammation. there are many reports examining the relationship between colonization by microorganisms in the stomach and oral cavity. we found that some oral bacteria are able to trap h. pylori cells, but oral bacteria inhibit h. pylori growth in vi ... | 2003 | 12593607 |
| monocyte-derived rantes is intrinsically elevated in periodontal disease while mcp-1 levels are related to inflammation and are inversely correlated with il-12 levels. | bacteria colonizing tooth surfaces are essential in the induction of an inflammatory response in the periodontal tissues, but do not cause periodontitis in everyone, implicating differences in the host immune response. these possible differences were studied using lipopolysaccharide (lps)-stimulated whole blood cell cultures (wbcc), which revealed a down regulation of monocyte derived interleukin-12 (il-12p70) in untreated periodontitis patients and an up regulation after therapy. il-12p70 is a ... | 2003 | 12605701 |
| chaperonin 60 unfolds its secrets of cellular communication. | the cell biology of the chaperonins (cpns) has been intensively studied over the past 25 years. these ubiquitous and essential molecules assist proteins to fold into their native state and function to protect proteins from denaturation after stress. the structure of the most widely studied cpn60, escherichia coli groel, has been solved and its mechanism of protein folding action largely established. but in the last decade, evidence has accumulated to suggest that the cpn60s have functions in add ... | 2002 | 12653476 |
| helicobacter pylori and campylobacter rectus share a common antigen. | aim: the aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human. materials and methods/results: among the putative periodontopathogens tested (actinobacillus actinomycetemcomitans, campylobacter rectus, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and treponema denticola), cross-reactive bands were only ... | 2003 | 12654095 |
| campylobacter gracilis and campylobacter rectus in primary endodontic infections. | a species-specific nested polymerase chain reaction (pcr) assay was used to investigate the occurrence of campylobacter gracilis and c. rectus in primary root canal infections. | 2003 | 12657143 |
| prevalence of cytolethal distending toxin production in periodontopathogenic bacteria. | cytolethal distending toxin (cdt) is a newly identified virulence factor produced by several pathogenic bacteria implicated in chronic infection. seventy three strains of periodontopathogenic bacteria were examined for the production of cdt by a hela cell bioassay and for the presence of the cdt gene by pcr with degenerative oligonucleotide primers, which were designed based on various regions of the escherichia coli and campylobacter cdtb genes, which have been successfully used for the identif ... | 2003 | 12682119 |
| bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer. | the goal of this study is to determine if the smear layer affects the passage of bacteria through or around obturating material as evidenced by penetration of bacteria through and out the canal. specifically, this study focused on determining the effect of the smear layer on the magnitude of bacterial penetration through the apical foramen. | 2003 | 12735922 |
| humoral immune responses to s-layer-like proteins of bacteroides forsythus. | bacteroides forsythus is one of the important periodontopathic bacteria, and this microorganism is known to have an s-layer outside the outer membrane. the s-layer-like antigens were recently isolated from b. forsythus, and they were found to be 270- and 230-kda proteins in the envelope fraction. in this study, these proteins were confirmed to be specific to b. forsythus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and they were clearly recognized by sera from patients with adul ... | 2003 | 12738635 |
| accidentally induced periodontitis in primary dentition: longitudinal examinations of periodontal bacteria and clinical conditions. | periodontitis is very rare in the primary dentition although it can be accidentally induced in young children. the purpose of this study was to describe a case of periodontitis in a 4-year-old child, which was accidentally induced by insertion of small plastic tubes into the dental cervix of the primary incisors during play, and to discuss the clinical, radiographic, and microbiological findings. removal of the plastic tubes resulted in resolution of the periodontal condition. prevotella nigresc ... | 2003 | 12752919 |
| periodontal pathogens in subgingival plaque of hiv-positive subjects with chronic periodontitis. | many putative periodontal pathogens associated with periodontal disease in human immunodeficiency virus (hiv)-infected patients also occur in non-hiv-infected individuals. this study examined the prevalence of eight periodontal pathogens in hiv-positive and hiv-negative patients with chronic periodontitis using the 16s rna polymerase chain reaction technique. the results showed a significant prevalence of porphyromonas gingivalis and treponema denticola among hiv-negative patients compared to hi ... | 2003 | 12753474 |
| can presence or absence of periodontal pathogens distinguish between subjects with chronic and aggressive periodontitis? a systematic review. | the purpose of this study was to determine to what extent the presence or absence of periodontal pathogens can distinguish between subjects with chronic and aggressive periodontitis. | 2002 | 12787203 |
| positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections. | dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ... | 2003 | 12877258 |
| subgingival microflora in turkish patients with periodontitis. | no information exists on periodontitis-associated subgingival microbiota from turkey. we determined the occurrence, interspecies relationships, and clonal characteristics for a group of periodontal bacteria in a turkish study population. | 2003 | 12886990 |
| localized aggressive periodontitis in primary dentition: a case report. | a 5-year-old japanese boy presented with persistent gingival inflammation and severe mobility of the right lower primary incisors. due to severe alveolar bone loss and a deep periodontal pocket (5 mm), the incisors were extracted at the second visit. | 2003 | 12931770 |
| potential role of vascular endothelial growth factor, interleukin-8 and monocyte chemoattractant protein-1 in periodontal diseases. | host-mediated immunoinflammatory pathways activated by bacteria lead to destruction of the periodontal connective tissues and alveolar bone. the objective of this study was to elucidate the activation of the inflammatory processes in periodontal disease by quantitative assessment of cytokines and periodontopathogens. gingival crevicular fluids (gcf) and subgingival plaque samples were collected from patients with chronic periodontitis and gingivitis and from periodontally healthy sites. vascular ... | 2003 | 12962428 |
| prevalence of periodontopathic bacteria in aggressive periodontitis patients in a japanese population. | actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis. other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis, although the evidence is controversial. the aim of the present study was to investigate the prevalence of periodontopathic bacteria and to clarify the microbiological features of aggressive periodontitis in japanese patients. | 2003 | 14653392 |
| evaluation of the mandibular third molar pericoronitis flora and its susceptibility to different antibiotics prescribed in france. | this work assessed the polymicrobial flora of mandibular third molar pericoronitis. obligate anaerobes were found in almost all cases (32 of 35). amoxicillin and pristinamycin were the most effective against the flora, particularly aerobic organisms. metronidazole alone or combined with spiramycin was the most effective drug against obligate anaerobes. | 2003 | 14662986 |