Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cns lyme disease. | 1992 | 1482459 | |
| subtelomeric expression regions of borrelia hermsii linear plasmids are highly polymorphic. | borrelia hermsii, a relapsing fever agent, undergoes multiphasic antigenic variation to evade its host's immune response. serotype specificity is determined by variable membrane lipoproteins, vmps, which are expressed from genes located near the end of a linear plasmid. using the polymerase chain reaction and primers representing the promoter of the active vmp and a conserved telomeric sequence, we characterized the subtelomeric expression regions of the 25 known serotypes of strain hs1. the dis ... | 1992 | 1484486 |
| [lyme borreliosis in childhood]. | ten years after detection of borrelia burgdorferi as the etiologic agent of lyme borreliosis and after introduction of serological methods an overview of the disease in children is given and compared with own observations. in view of the wide clinical spectrum and the difficulties in interpreting serological results the early diagnosis of lyme borreliosis is still problematic at present. on the other hand, in case of late diagnosis therapeutic failure and/or partial recovery may result. data of ... | 1992 | 1484707 |
| are csf or serum ganglioside antibodies related to peripheral nerve demyelination in neuroborreliosis, guillain-barré syndrome, or chronic inflammatory demyelinating polyradiculoneuropathy? | cerebrospinal fluid (csf) and serum igg and igm antibodies to seven gangliosides were determined in patients with neuroborreliosis (nb) (n = 20), guillain-barré syndrome (gbs) (n = 13), and chronic inflammatory demyelinating polyradiculoneuropathy (cidp) (n = 10). the incidence of elevated antibodies was highest in nb and lowest in cidp. correlation between csf and serum antibodies was only observed for igg antibodies to gm1, gd1b and gt1b in gbs. the strong igm antibody reactivity to gangliosid ... | 1992 | 1486101 |
| two-year survey of the incidence of lyme borreliosis and tick-borne encephalitis in a high-risk population in sweden. | a survey was made over a two-year period (september 1987 to august 1989) of a population living in an area endemic for lyme borreliosis and tick-borne encephalitis in sweden. for each patient a blood sample was collected and a questionnaire completed annually. all sera were tested for an antibody response to borrelia burgdorferi in an eia using sonicated antigen and for an antibody response to the tick-borne encephalitis virus using an eia and a haemagglutination inhibition test. antibodies to b ... | 1992 | 1486884 |
| risk factors for lyme disease in a small rural community in northern california. | a 1-year prospective study of risk factors for seropositivity to and contraction of lyme disease among members of a small rural community (population, approximately 150) was conducted in northwestern california in 1988-1989. the initial rate of seropositivity for borrelia burgdorferi for 119 current or former residents ranged from 15 to 20% among three laboratories, with statistically significant interlaboratory agreement. questionnaires were completed by 93 current residents at entry and 80 res ... | 1992 | 1488962 |
| mapping of genes on the linear chromosome of the bacterium borrelia burgdorferi: possible locations for its origin of replication. | molecular clones of borrelia burgdorferi, aetiologic agent of lyme borreliosis, were isolated and analysed by dna sequence determination. this procedure yielded b. burgdorferi homologues of gida, gyrb, gyra, ftsa and ftsz. the genes were located on the physical map of the b. burgdorferi linear chromosome. also mapped were the genes fla and p60 while dnaa was mapped using a heterologous probe. gyra and gyrb were found to be in tandem and were mapped, along with dnaa at the centre of the chromosom ... | 1992 | 1490605 |
| biopsy of canine ear tissue for detection of borrelia burgdorferi following ixodes dammini feeding. | a punch biopsy method was developed to detect and cultivate borrelia burgdorferi from canine ear tissue. the biopsy procedure was sensitive in that spirochetes were detected in 100% of the dogs upon which infected ticks had fed. b. burgdorferi could be cultured as early as 6 days after tick feeding at which time the organisms could not be concurrently recovered from peripheral blood. the ear punch biopsy provides a reliable method for sampling live canines and should prove useful for detecting e ... | 1992 | 1490617 |
| cdc should admit possibility of lyme. | 1992 | 1491660 | |
| research on ticks and tick-borne pathogens in missouri--an interim research report. | 1992 | 1491661 | |
| the enigma of lyme disease in missouri. | 1992 | 1491662 | |
| [three bacterial species associated with lyme borreliosis. clinical and diagnostic implications]. | dnas from various borrelia associated with lyme disease were reciprocally hybridized. this genomic taxonomy method showed that lyme disease agent comprised three genomic species. two species could be differentiated by phenotypic characters as major proteins molecular weights and monoclonal antibodies reactivity. western-blot with sera from patients suffering from different clinical forms of lyme disease: arthritis, meningoradiculitis and acrodermatitis chronicum atrophicans showed that each of t ... | 1992 | 1493569 |
| prevalence of lyme borreliosis spirochetes in ixodid ticks of japan, with special reference to a new potential vector, ixodes ovatus (acari: ixodidae). | to determine the vectors of lyme borreliosis in japan, the ixodid ticks taken in hokkaido, fukushima, nagano, ehime, and kochi prefectures were examined individually for spirochetal infections by culturing their midgut tissues in bsk medium. the cultivable spirochetes identified as borrelia burgdorferi were detected in ixodes persulcatus schulze adults (15.5% of 634), nymph (6.7% of 15) and ixodes ovatus neumann adult (9.6% of 188) from hokkaido, i. ovatus adults (25.5% of 47) from fukushima, an ... | 1992 | 1495032 |
| spatial analysis of the distribution of ixodes dammini (acari: ixodidae) on white-tailed deer in ogle county, illinois. | the pattern of infestations of ixodes dammini on white-tailed deer in ogle county in illinois was studied through examinations of hunted deer from 1988 to 1990. the illinois geographic information system mapped the spatial distribution of tick infestations on deer and related it to a known endemic focus for i. dammini and borrelia burgdorferi (castle rock state park), and to a major waterway (rock river). second-order neighborhood analysis was used to analyze the spatial distribution of deer aro ... | 1992 | 1495039 |
| rates of borrelia burgdorferi infection in ixodes dammini (acari: ixodidae) in southwestern wisconsin. | ixodes dammini spielman, clifford, piesman & corwin collected from a study site in southwestern wisconsin were examined for borrelia burgdorferi infection. adult spirochete infection rates varied seasonally (38.1%, spring 1990; 60.3%, fall 1990; 41.2%, spring 1991) and were similar to or higher than rates reported in various studies from the northeastern united states. statistical comparison of seasonal infection rates indicated a significant difference between the fall 1990 adult population and ... | 1992 | 1495049 |
| isolation of borrelia burgdorferi (spirochaetales: spirochaetaceae) from ixodes scapularis and dermacentor albipictus ticks (acari: ixodidae) in oklahoma. | borrelia burgdorferi was isolated from ixodes scapularis say and dermacentor albipictus packard that were removed as partially fed adults from white-tailed deer, odocoileus virginianus zimmermann, in oklahoma. isolation in media was accomplished only after homogenates of pooled field-collected ticks were inoculated into laboratory-reared peromyscus leucopus and reisolated from the urinary bladder into bsk ii media. both isolates were confirmed by western blot analysis and reactivity with monoclo ... | 1992 | 1495072 |
| cluster of tick-borne infections at fort chaffee, arkansas: rickettsiae and borrelia burgdorferi in ixodid ticks. | human intrusion into pristine habitats increases the likelihood of acquiring infectious agents from potentially infective ticks. as part of a larger human serological investigation into tick-borne illnesses, 3,000 ixodid ticks were collected during may, august, and november 1990 at fort chaffee, arkansas. ticks were examined to determine whether they harbor rickettsiae, ehrlichiae, and borrelia burgdorferi, and to assess relationship to human exposure to tick-borne infections at fort chaffee, ar ... | 1992 | 1495077 |
| attempted transmission of borrelia burgdorferi (spirochaetales: spirochaetaceae) (jdi strain) by ixodes scapularis (acari: ixodidae), dermacentor variabilis, and amblyomma americanum. | laboratory-reared ixodes scapularis say, amblyomma americanum (l.), and dermacentor variabilis (say) were fed on new zealand white rabbits experimentally infected with borrelia burgdorferi (jdi strain). at repletion, spirochetes could be detected by dark-field microscopy only in i. scapularis. acquisition rates were 18 and 21%. when previously exposed nymphs of each species were fed on susceptible rabbits, i. scapularis was the only tick of the three species that transmitted b. burgdorferi. when ... | 1992 | 1495078 |
| third-year evaluation of host-targeted permethrin for the control of ixodes dammini (acari: ixodidae) in southeastern connecticut. | the impact of commercially available permethrin-treated cotton balls targeted at ixodes dammini spielman, clifford, piesman & corwin on white-footed mice, peromyscus leucopus, was evaluated for a third year at five residential sites in south-central connecticut. each site had been treated twice each year from 1989 through 1991 with sufficient product to treat 0.4 ha of mouse habitat, and results were compared with five untreated sites. there were no significant differences in the number of host- ... | 1992 | 1495085 |
| gene conversion in the escherichia coli recf pathway: a successive half crossing-over model. | gene conversion--apparently non-reciprocal transfer of sequence information between homologous dna sequences--has been reported in various organisms. frequent association of gene conversion with reciprocal exchange (crossing-over) of the flanking sequences in meiosis has formed the basis of the current view that gene conversion reflects events at the site of interaction during homologous recombination. in order to analyze mechanisms of gene conversion and homologous recombination in an escherich ... | 1992 | 1495473 |
| immunisation of cattle against theileriosis in nakuru district of kenya by infection and treatment and the introduction of unconventional tick control. | one hundred and one cross european-boran cattle (50 cows and 51 calves), on a farm in nakuru district, kenya, were immunised against theileriosis using theileria parva lawrencei and theileria parva parva stocks from another district of kenya. the stabilates used were t.p.lawrencei (mara iii) used at 10(-1.7) dilution and t.p.parva (kilae) used at 10(-1.0) dilution. the stabilates were combined and inoculated simultaneously with a short-acting formulation of oxytetracycline hydrochloride given in ... | 1992 | 1496782 |
| localized scleroderma is not a borrelia burgdorferi infection in france. | 1992 | 1498396 | |
| borrelia burgdorferi stimulates release of interleukin-1 activity from bovine peripheral blood monocytes. | infection with borrelia burgdorferi is suspected to be a cause of lameness and arthritis in cattle. interleukin-1 (il-1) activity has been detected in joint fluids from human patients affected by various arthritides, including lyme arthritis. in addition, human monocytes and murine macrophages have been reported to release il-1 activity when incubated with b. burgdorferi in vitro. to address a possible mechanism by which b. burgdorferi might cause a bovine arthritic syndrome, we determined wheth ... | 1992 | 1500171 |
| roseolar lesions in lyme disease: isolation of the causative agent. | 1992 | 1500246 | |
| [lyme disease: 4 reported cases in the autonomous community of aragon]. | lyme's disease (ld) is a multisystemic infection due to borrelia burgdorferi transmitted through the byte of a vector arthropod of the ixodes genre. until now we do not know exactly which is the geographical distribution in our setting, no case have been published in the aragon community. we present four patients diagnosed of lyme's disease following clinical and serological criteria (ifi) according to the recommendations of the spanish group for the study of lyme's disease. a patient with chron ... | 1992 | 1502396 |
| carditis in lyme disease susceptible and resistant strains of laboratory mice infected with borrelia burgdorferi. | the clinical and pathologic evolution of cardiac lyme disease was evaluated in four-week-old susceptible c3h/he (c3h) and resistant c57bl/6 (b6) mice on days 3, 6, 10, 15, 30, 60, and 90 after intradermal inoculation with borrelia burgdorferi strain n40. culture, dna polymerase chain reaction, in situ nucleic acid hybridization, immunoperoxidase histochemical analysis, and silver stain were used to detect spirochetes. spirochetes were first detected by culture on day 6 in two of four c3h mice. t ... | 1992 | 1503192 |
| genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves borrelia burgdorferi into three distinct phyletic groups. | the causative agent of lyme disease, borrelia burgdorferi, was first identified by burgdorfer et al. in 1982 (w. burgdorfer, a. g. barbour, s. f. hayes, j. l. benach, e. grunwaldt, and j. p. davis, science 216:1317-1319, 1982) and was isolated by barbour et al. in 1983 (a. g. barbour, w. burgdorfer, s. e. hayes, o. peter, and a. aeschlimann, curr. microbiol. 8:123-126, 1983). since then, a large number of isolates have been collected, and there have been questions regarding the relationships amo ... | 1992 | 1503971 |
| immunogenicity and efficacy study of a commercial borrelia burgdorferi bacterin. | the immunogenicity and efficacy of a commercial borrelia burgdorferi bacterin was evaluated for stimulation of the host immune response and protection against clinical disease associated with experimentally induced borreliosis in dogs. a total of 30 vaccinated and 24 control dogs were used in 3 separate studies. the vaccine was given im as two 1-ml doses separated by a 3-week interval. two weeks or 5 months following the last vaccination, the dogs were challenge inoculated with 7 daily doses of ... | 1992 | 1506244 |
| use of a novel technique of cutaneous lavage for diagnosis of lyme disease associated with erythema migrans. | determining the microbial cause of cellulitis is often difficult. in this study, a novel two-needle lavage technique was used to culture borrelia burgdorferi from the skin of suspected erythema migrans lesions. | 1992 | 1507378 |
| specific antigen binding by activated cerebrospinal fluid b lymphocytes in acute neuroborreliosis. | a recently developed immunocytochemical antigen-binding sandwich test for the identification of specific activated b lymphocytes was applied to cerebrospinal fluid cells and peripheral blood mononuclear cells in patients with acute neuroborreliosis. discrimination of antigen-binding phagocytes was achieved by double staining with monoclonal antibodies. specific activated b lymphocytes were much more numerous in cerebrospinal fluid than in blood, showing great interindividual differences. when in ... | 1992 | 1512607 |
| borrelia burgdorferi infection in white-footed mice (peromyscus leucopus) in hemlock (tsuga canadensis) habitat in western pennsylvania. | white-footed mice (peromyscus leucopus) were captured and their tissues sampled from 27 sites in seven counties of western pennsylvania in 1990 for isolation and identification of borrelia burgdorferi. two hundred sixty mice were captured from which there were 27 isolations. significantly more mice were captured and significantly more isolations made from hemlock (tsuga canadensis) habitat than from deciduous species forest. hemlock habitat is sparse and focal but evidently increases winter surv ... | 1992 | 1512867 |
| borrelia sp. in ticks recovered from white-tailed deer in alabama. | six hundred sixty-five hunter-killed white-tailed deer (odocoileus virginianus) from 18 counties in alabama (usa) were examined for ticks. most of the collections were made at state-operated wildlife management areas. four species of ticks (n = 4,527) were recovered: the lone star tick amblyomma americanum (n = 482); the gulf coast tick a. maculatum (n = 11); the winter tick dermacentor albipictus (n = 1,242); and the black-legged tick ixodes scapularis (n = 2,792). fifty-six percent of the tick ... | 1992 | 1512879 |
| antibodies to borrelia burgdorferi in patients with carpal tunnel syndrome. | in a study of 94 consecutive patients with neurophysiologically verified carpal tunnel syndrome (cts) 7/94 had igg and 0/94 igm serum titers to borrelia burgdorferi above the 98th percentile value of age and sex matched controls (n = 127). the difference in prevalence of positive igg serum titers in patients, compared to controls, was not statistically significant. even in patients, living in an area, highly endemic for lyme borreliosis, routine serological screening for borrelia infection does ... | 1992 | 1519478 |
| absence of lyme borreliosis among patients with presumed bell's palsy. | in a prospective study, 69 patients with a presumed idiopathic (bell's) peripheral facial palsy were clinically and serologically evaluated for the presence of lyme borreliosis. in addition, their clinical spectrum was compared with clinical manifestations collected retrospectively in nine patients with symptomatic peripheral facial palsy due to lyme borreliosis. the seroprevalence of borrelia burgdorferi antibodies, determined by flagellum enzyme-linked immunosorbent assay, among 69 patients wi ... | 1992 | 1520085 |
| polymorphism of outer surface proteins of borrelia burgdorferi as a tool for classification. | a total of 23 isolates of borrelia burgdorferi were characterized by sds-page and immunoblot analysis. one isolate came from the csf of a lyme neuro-borreliosis patient in valais (switzerland) and 22 were tick isolates (2 from i. dammini of shelter island, usa and 20 from i. ricinus of valais, switzerland). based on the electrophoretic mobility of outer surface proteins (ospa and ospb), four groups of b. burgdorferi could be defined. group i isolates possess an ospa of 31 kd and an ospb of 34 kd ... | 1992 | 1520966 |
| borrelia burgdorferi infection in europe: an hla-related disease? | various studies in the united states and europe have established human leucocyte antigens (hla) cw3, dr2 and dr4 as risk factors for manifest borrelia burgdorferi infection or the development of chronic courses of lyme disease. other studies failed to confirm these findings. in the present study the frequencies of hla a, b, cw and dr were analysed in 283 persons from austria and germany with manifest b. burgdorferi infection. no statistically significant differences were found between patients a ... | 1992 | 1521884 |
| cellular immune reactivity to recombinant ospa and flagellin from borrelia burgdorferi in patients with lyme borreliosis. complexity of humoral and cellular immune responses. | patients with lyme borreliosis (lb) usually develop a vigorous t cell response against the causative pathogen borrelia burgdorferi, but little is known about the antigens recognized in the cellular response. therefore, t cell reactivities against whole bacteria, recombinant 31-kd (outer surface protein a, [ospa]), and 41-kd proteins (flagellin) from b. burgdorferi were studied in patients with lb, non-lb patients, and healthy donors. in parallel, specific antibodies were determined by western bl ... | 1992 | 1522216 |
| the western fence lizard sceloporus occidentalis: evidence of field exposure to borrelia burgdorferi in relation to infestation by ixodes pacificus (acari: ixodidae). | the role of the western fence lizard sceloporus occidentalis in the enzootiology of the lyme disease spirochete borrelia burgdorferi was evaluated in the hopland and ukiah areas of mendocino county, california. in 1989, half of 74 lizards collected monthly from april to october at hopland were infested by the immature western black-legged tick ixodes pacificus at a mean intensity of 6.0 ticks per lizard. the prevalence of infestation of lizards by immature i. pacificus (36 of 73) at ukiah was si ... | 1992 | 1524146 |
| new lyme disease vaccine for dogs. | 1992 | 1524271 | |
| polymyalgia rheumatica--a delayed sequelae of borrelia infection? | 1992 | 1525640 | |
| delayed dissemination of lyme disease spirochetes from the site of deposition in the skin of mice. | to determine whether the agent of lyme disease disseminates in vertebrate hosts directly after deposition by an infecting tick, a 6-mm disk of skin was excised from the sites where nymphal ixodes dammini ticks infected by lyme disease spirochetes, borrelia burgdorferi, had fed. infection in each mouse was tested by examining xenodiagnostic ticks that had engorged on these mice 4 weeks later and by serologic testing. generalized infection was aborted when the site of inoculation was excised withi ... | 1992 | 1527418 |
| lymphocyte proliferation assay in lyme disease. | 1992 | 1527433 | |
| immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia. | we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ... | 1992 | 1530106 |
| neuroactive kynurenines in lyme borreliosis. | although neurologic dysfunction occurs frequently in patients with lyme borreliosis, it is rarely possible to demonstrate the causative organism within the neuraxis. this discordance could arise if neurologic symptoms were actually due to soluble neuromodulators produced in response to infection. since immune stimulation is associated with the production of quinolinic acid (quin), an excitotoxin and n-methyl-d-aspartate (nmda) agonist, we measured levels of csf and serum quin, and lymphokines. s ... | 1992 | 1531156 |
| nonspecific proliferative responses of murine lymphocytes to borrelia burgdorferi antigens. | proliferative responses of naive splenocytes to borrelia burgdorferi antigens from mice susceptible (c3h) and resistant (balb) to lyme borreliosis were investigated. b. burgdorferi spirochetes and recombinant outer surface proteins, ospa and ospb, were found to induce nonspecific proliferation of naive splenocytes from both strains of mice. cell purification studies localized nonspecific proliferation to the b cell-enriched fraction. b. burgdorferi, ospa, and ospb were found to induce igm and ig ... | 1992 | 1531672 |
| interleukin-1 (il-1) receptor blockade reduces endotoxin and borrelia burgdorferi-stimulated il-8 synthesis in human mononuclear cells. | interleukin-1 (il-1) is a potent stimulator of il-8 production by fibroblasts and monocytes. in the present study, we asked how much of endotoxin (lps)-induced il-8 production by human peripheral blood mononuclear cells was due to il-1 induced by lps. cells were stimulated with either il-1 beta, lps, or borrelia burgdorferi, and total il-8 was determined by a specific radioimmunoassay. the addition of saturating concentrations of il-1 receptor antagonist protein (irap) reduced the il-1 beta-, lp ... | 1992 | 1532945 |
| [lyme's disease, a possible cause of isolated acute myocarditis]. | the frequency of cardiac lesion in patients with other signs of lyme disease has been estimated at 8 percent. the usual manifestation of myocardial involvement is a varying degree of atrioventricular block or more diffuse signs of myocarditis. autopsy or intramyocardial biopsy provides a histological diagnosis of myocarditis. microscopy shows a diffuse lympho-plasmocytic infiltrate with presence of macrophages in the myocardium, associated with a varying number of necrotic myocytes. structures r ... | 1992 | 1535149 |
| light chain distribution of anti-borrelia burgdorferi igg antibodies in lyme arthritis. | joint fluid (jf) and serum igg from eight lyme arthritis patients was investigated by isoelectric focusing, blotting to membranes of uncoated nitrocellulose (nc) as well as borrelia (b.) burgdorferi antigen-coated nc (immunoblot) and immunoezymatic staining for visualization of the igg isotype and characterization of the kappa/lambda light chain distribution. oligoclonal bands of total igg occurred in jf as well as in serum (3 cases) or in jf only (1 case); igg lambda components were present in ... | 1992 | 1535989 |
| complement-mediated in vitro bactericidal activity of monoclonal antibodies reactive with outer-surface-protein ospb of borrelia burgdorferi. | murine monoclonal antibodies (mabs) were obtained against the outer-surface-protein ospa and ospb and against the 41-kda flagellar antigen of borrelia burgdorferi. the specificity of mab was determined by the western blotting technique and the surface association of the antigens was inferred by immunofluorescence of living bacteria. in an in vitro assay in the presence of complement, two mabs reactive with the ospa were able to kill borreliae, whereas several mabs reactive with the ospa as well ... | 1992 | 1537543 |
| cultivation of borrelia burgdorferi from erythema migrans lesions and perilesional skin. | skin biopsy specimens from the peripheral aspect of erythema migrans lesions (site 1) and from clinically normal perilesional areas (site 2) were compared as sources of borrelia burgdorferi. this spirochete was isolated from the skin of 18 of 21 (86%) patients with untreated early lyme disease at one or both biopsy sites. site 1 specimens were superior to site 2 specimens for the isolation of b. burgdorferi. site 1 specimens from 18 (86%) patients were culture positive, and site 2 specimens from ... | 1992 | 1537904 |
| serodiagnosis of lyme borreliosis by western immunoblot: reactivity of various significant antibodies against borrelia burgdorferi. | the significance of various antibodies against borrelia burgdorferi was studied by western blot (immunoblot) by using 578 human serum samples. the proteins regularly detected by using samples from patients with lyme borreliosis were those with bands with molecular masses of 94, 83, 75, 66, 60, 55, 46, 41, 39, 34, 31, 29, 22, and 17 kda. the detectable frequencies of most of these proteins appeared to be significantly different between the sera from patients with lyme borreliosis and those from n ... | 1992 | 1537905 |
| capacity of european animals as reservoir hosts for the lyme disease spirochete. | the abundance of spirochete-infected ixodes ricinus ticks naturally derived from endemic rodents was compared to identify the reservoir hosts of the agent of lyme disease at a series of enzootic sites in central europe. black-striped mice appear to be the most important rodent host for the tick and infect more ticks with borrelia burgdorferi than do other mice and voles tested. although rodents infect about half of the ticks that feed on them, lizards infect none. these spirochete-incompetent ho ... | 1992 | 1538153 |
| cultivation of borrelia burgdorferi from the blood and a subcutaneous lesion of a patient with relapsing febrile nodular nonsuppurative panniculitis. | 1992 | 1538171 | |
| evaluation of granulocytic ehrlichiosis in dogs of missouri, including serologic status to ehrlichia canis, ehrlichia equi and borrelia burgdorferi. | canine granulocytic ehrlichiosis was diagnosed in 37 dogs by finding ehrlichial morulae in 0.1 to 26.2% of their blood neutrophils and eosinophils. all 37 dogs had clinical signs of arthritis or muscular stiffness. titer to ehrlichia canis was determined in sera from 31 of the 37 dogs; 25 dogs had titer ranging from 1:20 to 1:5,120. in the other 6 dogs, titer to e canis was less than 1:10. the most common hematologic abnormality in these dogs, other than rickettsiemia, was thrombocytopenia. gran ... | 1992 | 1539918 |
| in vitro and in vivo induction of tumor necrosis factor alpha by borrelia burgdorferi. | tumor necrosis factor alpha (tnf-alpha) is an immunoregulatory cytokine with many biological activities including the mediation of inflammation. we examined sera and synovial fluids from patients seropositive for infection with borrelia burgdorferi using a radioimmunoassay specific for tnf-alpha. significant elevation of tnf-alpha was found in the sera and synovial fluids of patients examined, while controls showed no elevation. sera of mice infected with b. burgdorferi contained elevated levels ... | 1992 | 1541526 |
| long-term protection of mice from lyme disease by vaccination with ospa. | mice vaccinated with recombinant outer surface protein a (ospa) have been shown to be protected from infection with borrelia burgdorferi, the agent of lyme disease, when sacrificed 14 days after challenge with an intradermal inoculum of the spirochete. to determine whether infection was not merely delayed and that protection was long-lasting, we sacrificed vaccinated mice 60, 120, and 180 days after challenge; and to determine whether vaccinated mice retained their immune state over long periods ... | 1992 | 1541551 |
| interactions between extracellular borrelia burgdorferi proteins and non-borrelia-directed immunoglobulin m antibodies. | previous work showed that outer surface protein a (ospa) and ospb of borrelia burgdorferi may occur within an extracellular multiprotein complex, which was resolved by electrophoresis as an 83-kda major extracellular protein band. to characterize the 83-kda band, we sequenced the n terminus of the predominant peptide in the band and examined the interaction between the associated proteins. peptide sequence and amino acid composition comparisons showed identity with the heavy chain of immunoglobu ... | 1992 | 1541558 |
| borrelia burgdorferi strain 25015: characterization of outer surface protein a and vaccination against infection. | mice vaccinated with outer surface protein a (ospa) from borrelia burgdorferi strain n40 are protected from challenge with an intradermal syringe inoculum of b. burgdorferi strains n40, b31, and cd16. vaccination experiments were done to determine if protection extended to strains 297 and 25015. we now show that ospa-n40 immunized mice are protected against challenge with strain 297, isolated from the cerebrospinal fluid of a patient with neuroborreliosis, but not against challenge with strain 2 ... | 1992 | 1545130 |
| population genetic analysis of borrelia burgdorferi isolates by multilocus enzyme electrophoresis. | fifty borellia burgdorferi strains isolated from humans and ticks in europe and the united states were analyzed by multilocus enzyme electrophoresis. eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. ten loci were polymorphic. the average number of alleles per locus was 5.9, with a mean genetic diversity of 0.673 among electrophoretic types (ets). the strains were grouped into 35 ets constituting three main divisions (i, ii, and iii) separat ... | 1992 | 1548090 |
| [pigmented urticarial erythema with flaccid atrophy as expression of an unusual manifestation of lyme borreliosis]. | unusual courses of infections with borrelia burgdorferi can make diagnosis problematic, but early and adequate therapy is mandatory to avoid further complications. in this paper a patient is presented who developed urticarial erythemas and hyperpigmentation with slack skin atrophy. positive serological findings and the regression of the skin eruptions under antibiotic therapy confirmed the diagnosis of an unusual manifestation of lyme borreliosis. | 1992 | 1548134 |
| relationship between development of antibodies to borrelia burgdorferi in dogs and the subsequent development of limb/joint borreliosis. | the relationship between antibody production and the subsequent development of limb/joint disorders of borreliosis was examined in dogs from south central connecticut. dogs without signs of illness, determined by physical examination, were selected from dogs being tested for dirofilaria immitis. an elisa was used to detect antibodies to borrelia burgdorferi in 234 apparently healthy dogs during 1988. these dogs were monitored for 20 months after initial analyses to determine the prevalence of li ... | 1992 | 1548169 |
| clinically silent infections in patients with oligoarthritis: results of a prospective study. | oligoarticular synovitis of undetermined origin can closely resemble an incomplete form of reactive arthritis/reiter's syndrome. eighty three patients with oligoarthritis of undetermined origin were studied prospectively to identify asymptomatic infections potentially triggering the inflammatory response in the synovial fluid. at the time of initial evaluation, 57 (69%) of the patients with oligoarthritis and 4/20 (20%) of the control subjects were carriers of clinically silent infections. evide ... | 1992 | 1550413 |
| lyme disease in children: diagnosis, treatment, and prevention. | lyme disease is a multisystem disorder that is caused by infection with borrelia burgdorferi. in endemic areas, its occurrence is extremely common among children. the early diagnosis and treatment of lyme disease may prevent the development of serious cardiac, rheumatological, and neurological sequelae. for this reason, a full understanding of the clinical manifestations, laboratory evaluation, and antibiotic therapy of lyme disease is of vital importance. | 1992 | 1550714 |
| localized cutaneous scleroderma. | localized scleroderma in children includes morphea mainly on the trunk and linear scleroderma on the limbs, scalp, and face. progressive systemic sclerosis is very rare in children and change from localized to progressive disease is extremely rare. laboratory abnormalities occur with localized scleroderma, including eosinophilia, positive antinuclear factor (anf), and increased immunoglobulin (ig)g. the diseases are usually self limited, but involvement over bones may lead to marked functional i ... | 1992 | 1550717 |
| dna analysis of borrelia burgdorferi nch-1, the first northcentral u.s. human lyme disease isolate. | the dna of the first northcentral united states human lyme disease isolate, borrellia burgdorferi nch-1, was characterized and compared with the dnas of nine other b. burgdorferi isolates. strain nch-1 was isolated in august 1989 from a human skin biopsy specimen. dna was analyzed by pulsed-field gel electrophoresis and restriction endonuclease analysis. contour-clamped homogeneous electric field pulsed-field gel electrophoresis of in situ-lysed cells was performed to compare the plasmid profile ... | 1992 | 1551988 |
| analysis of leptospira spp., leptonema illini, and rickettsia rickettsii for the 39-kilodalton antigen (p39) of borrelia burgdorferi. | five serovars of leptospira interrogans, leptospira biflexa, leptonema illini, and rickettsia rickettsii were examined and found not to contain the 39-kda antigen (p39) of borrelia burgdorferi, the lyme disease spirochete. the specificity of this antigen and its reactivity with human lyme disease sera should exclude the possibility of false-positive serum samples from patients having had either leptospirosis or rocky mountain spotted fever, as well as tick-borne relapsing fever and syphilis, as ... | 1992 | 1551994 |
| polymerase chain reaction confirmation of borrelia burgdorferi in benign lymphocytic infiltrate of dermis. | 1992 | 1552070 | |
| distribution, density, and lyme disease spirochete infection in ixodes dammini (acari: ixodidae) on white-tailed deer in maryland. | a statewide survey of ticks parasitizing white-tailed deer was carried out in maryland during november 1989 to assess the status of the deer tick, ixodes dammini spielman, clifford, piesman & corwin, the major vector of lyme disease in the northeastern united states. ticks were collected from deer carcasses brought in by hunters at 23 check stations (one per county). a total of 3,437 i. dammini were collected from 538 of 1,281 deer (42%), together with 2,013 dermacentor albipictus (packard) and ... | 1992 | 1552529 |
| [the characterization of a spanish strain of borrelia burgdorferi]. | the characteristics of a spanish strain of borrelia burgdorferi, the spirochete which causes lyme's disease, and which, up to the present, has not been isolated in spain, are described. | 1992 | 1552756 |
| [peripheral facial paresis as a symptom of borrelia burgdorferi infection]. | infection with the tick-borne borrelia burgdorferi can lead to a variety of neurologic symptoms, the most frequent being a radicular pain syndrome due to meningitis. general symptoms such as asthenia or headache are also frequent, however, and serious neurologic complications such as dementia or spastic paresis may occur. at an early stage, borrelia infections can be easily treated with antibiotics, which makes it important to recognize the symptoms and make the correct diagnosis. a common featu ... | 1992 | 1553645 |
| structural analysis of an outer surface protein from the lyme disease spirochete, borrelia burgdorferi, using circular dichroism and fluorescence spectroscopy. | the etiological agent of lyme disease is the tick-borne spirochete, borrelia burgdorferi. a major antigen of b. burgdorferi is a 31 kda lipoprotein called outer surface protein a (ospa). recently, a truncated form of ospa (lacking 17 amino acids at the n-terminus) was cloned, expressed and purified in large quantities (dunn, j.j., lade, b.a. and barbour, a.g. (1990) protein expression and purification 1, 159-168). the truncated protein (ospa-257) is water-soluble, retains the ability to bind ant ... | 1992 | 1554741 |
| purification and characterization of a tryptic peptide of borrelia burgdorferi flagellin, which reduces cross-reactivity in immunoblots and elisa. | in man the early immune response in lyme disease is primarily directed against the endoflagellin antigen. isolated flagellar protein of borrelia burgdorferi suggests itself as a suitable test antigen. however, cross-reactivity between flagellins of b. burgdorferi, escherichia coli, bacillus subtilis, proteus mirabilis and salmonella typhimurium was demonstrated by immunoblotting and elisa with polyclonal rabbit-hyperimmune-sera. tryptic cleavage of recombinant b. burgdorferi 41 kda flagellin, ex ... | 1992 | 1556546 |
| adherence and entry of borrelia burgdorferi in vero cells. | adherence to and entry of the parasite into the host is one of the essential elements of microbial pathogenicity. we investigated the adherence to and entry into primate kidney epithelial (vero) cells of borrelia burgdorferi by radiolabelling techniques, immunofluorescence and electronmicroscopy. the attachment to and subsequent entry of both untreated and heat (50 degrees c)-treated b. burgdorferi into vero cells occurred at cell-surface sites associated with aggregated coated pits. in contrast ... | 1992 | 1560445 |
| molecular analysis and expression of a borrelia burgdorferi gene encoding a 22 kda protein (pc) in escherichia coli. | we describe the cloning and expression of the pc gene which encodes a major immunodominant protein of borrelia burgdorferi, the causative agent of lyme borreliosis. the pc protein was purified from lysates of b. burgdorferi strain pko. after tryptic digestion of the pc protein the resulting oligopeptides were applied to a gas-phase sequenator. thus partial amino acid sequences were obtained. the deduced oligonucleotides were used as hybridization probes. after southern blotting a reactive band i ... | 1992 | 1560779 |
| heterogeneity of outer membrane proteins in borrelia burgdorferi: comparison of osp operons of three isolates of different geographic origins. | biochemical and immunochemical studies of the outer membrane proteins of borrelia burgdorferi have shown that the ospa and ospb proteins from strains of different geographic origins may differ considerably in their reactivities with monoclonal antibodies and in their apparent molecular weights. to further characterize this variation in osp proteins between strains, the osp operons and deduced translation products from two strains, one from sweden (acai) and one from eastern russia (ip90), were s ... | 1992 | 1563773 |
| nucleotide sequence of the ospab operon of a borrelia burgdorferi strain expressing ospa but not ospb. | the nucleotide sequence of a 1.6-kb clone containing the gene for outer surface protein a (ospa) of a german strain (go2) of borrelia burgdorferi was determined. the deduced amino acid sequence showed a homology of 82% to the ospa molecules from three other b. burgdorferi strains. the best-conserved region was recognized at the 36-amino-terminal amino acids of ospa. ospb could not be identified in the strain investigated, probably because the nucleotide sequence of the ospab operon prevented exp ... | 1992 | 1563775 |
| an initial investigation of the status of borrellia burgodorferi and its suspected primary vector, ixodes scapularis, in south carolina. | based on a survey of hunter-harvested deer, the suspected primary vector of lyme disease in the southeast, i. scapularis, is most prevalent in sandhill and coastal plain counties of south carolina. none of 271 i. scapularis examined were found to be infected with the lyme disease spirochete. however, many more specimens of i. scapularis, a. americanum, and other tick species must be examined before an accurate estimate of the probability of acquiring lyme disease in south carolina can be made. | 1992 | 1564909 |
| lyme disease in dogs in spain. | 1992 | 1566536 | |
| tandem repeat of the 23s and 5s ribosomal rna genes in borrelia burgdorferi, the etiological agent of lyme disease. | the dna fragments containing the rrl and rrf genes were subcloned from a embl3 recombinant phage of borrelia burgdorferi strain b31 into puc18 and were characterized by restriction analysis and southern hybridization. a fine restriction map of the fragments was constructed and the organization of the genes was determined. the genomic hybridization using the gene probes from b. burgdorferi showed that there are two sets of rrl/rrf genes in that genome. the results also revealed the important fact ... | 1992 | 1567408 |
| molecular diagnosis of borrelia burgdorferi infection (lyme disease). | in spite of significant advances in immunologically based testing, accurate diagnosis of lyme borreliosis remains problematic. to address this issue, a dna amplification-based diagnostic test was developed utilizing the polymerase chain reaction (pcr) and oligonucleotide primers specific for the ospa and ospb genes of borrelia burgdorferi. in this approach, a relatively large dna fragment is amplified with an outer set of primers, and a "nested" internal sequence of the pcr product subsequently ... | 1992 | 1567553 |
| detection of plasma tumor necrosis factor, interleukins 6, and 8 during the jarisch-herxheimer reaction of relapsing fever. | the jarisch-herxheimer reaction (j-hr) is a clinical syndrome occurring soon after the first adequate dose of an antimicrobial drug to treat infectious diseases such as lyme disease, syphilis, and relapsing fever. previous attempts to identify factors mediating this reaction, that may cause death, have been unsuccessful. we conducted a prospective trial in addis ababa, ethiopia on 17 patients treated with penicillin for proven louse-borne relapsing fever due to borrelia recurrentis to evaluate t ... | 1992 | 1569394 |
| identification of the tick-borne relapsing fever spirochete borrelia hermsii by using a species-specific monoclonal antibody. | borrelia hermsii causes a relapsing fever in humans and is one of several species of tick-borne spirochetes known to occur in the western united states. spirochetes observed in the peripheral blood of patients acutely ill have been presumptively identified in the past by the geographic location of exposure and the probable species of tick vector. we describe a monoclonal antibody (h9826) that bound to the flagellar protein of b. hermsii but not to those of any of the other species tested, which ... | 1992 | 1572965 |
| untreated neuroborreliosis: bannwarth's syndrome evolving into acute schizophrenia-like psychosis. a case report. | in general, meningopolyradiculitis (bannwarth's syndrome, stage 2 of neuroborreliosis) follows a predictable monophasic self-limiting course. in contrast, we report the case of a patient with an untreated meningopolyradiculitis which evolved into acute schizophrenia-like psychosis due to persistent infection with borrelia burgdorferi. the psychosis resolved within 1 week of treatment with ceftriaxone. this case shows that the usually benign monophasic meningopolyradiculitis may progress to sever ... | 1992 | 1573415 |
| deer, ticks, and lyme disease. | 1992 | 1574226 | |
| incidence and prevalence of lyme disease in a suburban westchester county community. | this study was designed to determine the incidence and prevalence of lyme disease in a section of chappaqua, ny, a residential community in which lyme disease is epidemic, and to identify risk factors for this disease. on the basis of clinical history and baseline serologic testing, the overall prevalence of lyme disease for 114 persons entering the study was 8.8%. the incidence during the 5-month study period of may through september 1989 was 2.6%; all three incident cases had erythema migrans ... | 1992 | 1574232 |
| lyme disease case studies. | lyme disease is the commonest tick-borne disease in the united states. since the mill creek clinic opened in march 1991, we have diagnosed two cases of the total five cases of lyme disease reported in west virginia. | 1992 | 1574875 |
| diagnosing lyme disease. | the incidence of lyme disease is increasing, and the disease is spreading geographically. prompt diagnosis requires recognition of characteristic signs and symptoms of infection with the spirochete borrelia burgdorferi. in more than half of cases, erythema migrans is the earliest sign of lyme disease. although less frequently seen than erythema migrans, peripheral neuropathy of the seventh cranial nerve is another important sign. tests for lyme disease await refinement, but laboratory evaluation ... | 1992 | 1575110 |
| [nodular panniculitis: a manifestation of lyme borreliosis?]. | infection with borrelia burgdorferi can induce various skin manifestations. the type of skin manifestation and the histopathological picture depend on the stage of infection and vary from local inflammatory infiltrates to chronic atrophic skin disease. involvement of subcutaneous tissue has been observed only very rarely. we report on two patients suffering from nodular panniculitis (pfeifer-weber-christian) and present evidence that the disease was caused by borrelia burgdorferi. in one of the ... | 1992 | 1577601 |
| [neurosyphilis--a disease imitator still of current interest]. | 1992 | 1579000 | |
| complications of lyme borreliosis. | lyme borreliosis is the multisystem infectious disease caused by the spirochete borrelia burgdorferi. complications of this infection can involve many organ systems, especially the skin, joints, nervous system, and heart. these manifestations may be acute, or evolve slowly over months or years. diagnosis is not always straightforward, and is currently hampered by lack of a specific serologic assay. this review discusses the syndromes associated with lyme borreliosis and addresses issues of diagn ... | 1992 | 1580609 |
| [borrelia burgdorferi may be the causal agent of sarcoidosis]. | serum antibody to borrelia burgdorferi was measured in 33 patients with sarcoidosis who were confirmed clinically and pathologically. the results showed that 81.8% of the patients were positive. in addition, a strain of borrelia burgdorferi was isolated from a patient's blood. fourteen patients received ceftriaxone 2 g per day and/or penicillin 12 million per day and a patient received lincomycin 1.2 g per day. the antibody titer of the patients turned to normal level, their sace turned to norma ... | 1992 | 1582344 |
| seroepidemiological studies of borrelia burgdorferi infection in sheep in norway. | an enzyme-linked immunosorbent assay for antibodies to borrelia burgdorferi in sheep was established. the assay was used in a seroepidemiological survey in which serum samples from 327 norwegian rams were screened for antibodies to b. burgdorferi. these rams were randomly chosen, clinically healthy animals from all parts of norway. they came both from areas where ixodes ricinus is common and from areas where the tick has not been found. the age of the animals varied from 0.5 to 7.5 years. sera f ... | 1992 | 1583130 |
| serologic diagnosis of lyme disease using recombinant outer surface proteins a and b and flagellin. | recombinant (r) outer surface proteins (osp) a and b, flagellin, and an immunogenic region of flagellin (41-g) from borrelia burgdorferi were evaluated using immunoblot and elisa for usefulness as substrates in diagnostic testing for lyme disease. antibodies to rospa, rospb, and r-flagellin were detected by immunoblot and elisa using the recombinant proteins. patients with late disease responded to rospa, rospb, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed ... | 1992 | 1583333 |
| prevalence of antibody to borrelia burgdorferi by indirect fluorescent antibody assay, elisa, and western immunoblot in healthy adults in wisconsin and arizona. | the prevalence of antibody to borrelia burgdorferi in healthy adults from wisconsin and arizona was determined by indirect fluorescent antibody assay (ifa), elisa, and western immunoblotting. a total of 301 sera from adult volunteer blood donors were collected from three areas of wisconsin and compared with 49 consecutive anonymous adult volunteer donor sera from tucson, arizona, an area without reported lyme borreliosis. regional differences in seropositivity were found for western immunoblotti ... | 1992 | 1583334 |
| lyme neuroborreliosis manifesting as an intracranial mass lesion. | lyme neuroborreliosis is one of the chronic manifestations of lyme disease and is caused by the neurotropic spirochete, borrelia burgdorferi. two of the three stages of lyme disease potentially involve the central nervous system: a second stage that may manifest as meningitis, cranial neuritis, or radiculoneuritis; and a third stage, or chronic neuroborreliosis, with parenchymal involvement. the tertiary stage may mimic many conditions, including multiple sclerosis, polyneuropathy, viral encepha ... | 1992 | 1584393 |
| [lyme borreliosis in an endemic region in europe. prevalence of antibodies and clinical spectrum]. | a representative sample of the population of two villages located in an area of north baden where borreliosis is endemic was studied to ascertain the prevalence of antibodies against borrelia burgdorferi and of clinical abnormalities. the aim of the study was to determine what clinical symptoms and signs were correlated with positive antibody status. out of the 2928 inhabitants 1228 were investigated, and 207 of these (16.9%) were seropositive. certain clinical abnormalities showed highly signif ... | 1992 | 1587208 |
| antibiotic treatment of lyme disease. current recommendations by stage and extent of infection. | much has been learned about lyme disease over the past several years, but much remains to be learned. careful clinical observation has led to elucidation of the natural history of this disease, and further clinical observations are needed to unravel the remaining areas of uncertainty. it is by no means clear that all the symptoms attributed to lyme disease today actually represent true manifestations of borrelia burgdorferi infection or that patients with well-documented lyme disease whose sympt ... | 1992 | 1589368 |
| special concerns in lyme disease. seropositivity with vague symptoms and development of fibrositis. | fear of lyme disease may be as powerful as the disease itself. patients may insist on being tested for infection although little evidence of it exists, and a positive result in the face of vague symptoms can add to the problem. physicians should explain to these patients the differences in "background" seropositivity in various geographic locations and the drawbacks of instituting unnecessary treatment. fibrositis may evolve over time after lyme disease infection. many factors may trigger this d ... | 1992 | 1589369 |
| physical map of the linear chromosome of the bacterium borrelia burgdorferi 212, a causative agent of lyme disease, and localization of rrna genes. | the spirochete borrelia burgdorferi, which causes lyme disease, and other members of the borrelia genus are unique among characterized bacteria in having a linear chromosome. a restriction map of the chromosome of b. burgdorferi 212 was constructed by making extensive use of digestions in agarose blocks of restriction endonuclease fragments or chromosomal dna that had been purified by pulsed-field gel electrophoresis. a total of 47 digestion sites for the enzymes sgrai, sacii, mlui, bsshii, eagi ... | 1992 | 1592827 |
| identification of a third genomic group of borrelia burgdorferi through signature nucleotide analysis and 16s rrna sequence determination. | as part of a continuing effort to assess genetic variation among isolates of borrelia burgdorferi we have determined the 16s rrna signature nucleotide makeup of two tick isolates from the ussr. signature nucleotides were identified via reverse transcriptase primer extension sequencing of select regions of the 16s rrna molecule. in addition, the near complete 16s rrna sequence of one of the isolates, r-ip3, was determined and utilized in a phylogenetic assessment. the sequence was aligned with th ... | 1992 | 1593264 |