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experimental infection of akodon molinae (rodentia, cricetidae) with junín virus.experimental infection with the xj-clone 3 strain of junín virus in laboratory bred akodon molinae, a cricetid rodent inhabiting the borders of endemic argentine hemorrhagic fever areas, was studied. suckling animals inoculated intracerebrally proved sensitive and became chronically infected. sixty percent of the rodents showed neurologic involvement, with mortality reaching 60%. virus was recovered from the brain at 7, 15, 21, 37, and 57 days postinfection (pi). by immunofluorescence (if), vira ...19863009705
experimental infection of akodon molinae (rodentia, cricetidae) with junín virus.experimental infection with the xj-clone 3 strain of junín virus in laboratory bred akodon molinae, a cricetid rodent inhabiting the borders of endemic argentine hemorrhagic fever areas, was studied. suckling animals inoculated intracerebrally proved sensitive and became chronically infected. sixty percent of the rodents showed neurologic involvement, with mortality reaching 60%. virus was recovered from the brain at 7, 15, 21, 37, and 57 days postinfection (pi). by immunofluorescence (if), vira ...19863009705
junin virus access to cns by extraneural rat inoculation.this study was carried out to determine the pathways along which two strains of junin virus (jv), the pathogenic xjv and the attenuated xjc13v, reach the cns following ip inoculation of 2-day-old rats. a sequential study of infectivity and antigen distribution in peritoneal macrophages, spleen, and brain was performed. mortality was 85% with the former strain, but only 15% with the latter. at 4-7 days pi, xjv-infected animals had viral antigen in 10% of peritoneal macrophages. viremia and spleen ...19863009706
effect of ribavirin and tributylribavirin on argentine hemorrhagic fever (junin virus) in guinea pigs.subcutaneous injections of ribavirin into guinea pigs infected intraperitoneally or intracerebrally with junin virus significantly increased the mean time to death but did not enhance survival of the animals. we found similar results with tributylribavirin. virus replication was delayed, but not prevented, in ribavirin-treated infected guinea pigs. the animals usually died with high virus titers in their brains and frequently were paralyzed.19863013087
effect of ribavirin and tributylribavirin on argentine hemorrhagic fever (junin virus) in guinea pigs.subcutaneous injections of ribavirin into guinea pigs infected intraperitoneally or intracerebrally with junin virus significantly increased the mean time to death but did not enhance survival of the animals. we found similar results with tributylribavirin. virus replication was delayed, but not prevented, in ribavirin-treated infected guinea pigs. the animals usually died with high virus titers in their brains and frequently were paralyzed.19863013087
junin virus isolation from lympho-mononuclear cells of patients with argentine hemorrhagic fever.detection of viremia was attempted by three different methods in 30 cases of argentine hemorrhagic fever (ahf). cocultivation of peripheral blood mononuclear cells (pbmc) with vero cell monolayers was the most sensitive, detecting junin virus (jv) in 96% of the cases. inoculation of whole blood into suckling mice and on vero cells rendered 53 and 46% of positive isolations, respectively. the results presented suggest that pbmc are infected with jv during the acute period of ahf. jv was isolated ...19863013799
relationship between junin virus infection of thymus and the establishment of persistence in rodents. 19863014287
heterogeneity of junin virus strains. 19863014289
damage of human polymorphonuclear leukocytes by junin virus. 19863014291
effect of ribavirin and immune serum on junin virus-infected primates. 19863014292
photodynamic inactivation of junin virus. 19863014294
antigenic variants of junín virus isolated from infected calomys musculinus. brief report.junín virus establishes a long-term persistent infection in its natural host, calomys musculinus. virus recovered from blood of infected animals from 14 to 61 days p.i. behaved antigenically distinct to parental virus, as shown by cross neutralization assays. the emergence of antigenic viral variants occurs during both the acute and persistent state of infection.19863015087
antigenic variants of junín virus isolated from infected calomys musculinus. brief report.junín virus establishes a long-term persistent infection in its natural host, calomys musculinus. virus recovered from blood of infected animals from 14 to 61 days p.i. behaved antigenically distinct to parental virus, as shown by cross neutralization assays. the emergence of antigenic viral variants occurs during both the acute and persistent state of infection.19863015087
macrophage maturity and modulation of response to junín virus in infected rats.replication of junín virus in peritoneal macrophages from newborn rats was greater for prototype strain xj than for strain xjc13, whereas in cells from adult animals viral multiplication proved minimal. transfer of peritoneal adherent cells from normal adult to strain xj-infected newborn rats lowered mortality significantly. silica blockade of macrophages protected two-day-old strain xj-infected animals and depressed brain titers of virus significantly, whereas treatment had no effect on strain ...19863016114
partial characterization of two temperature-sensitive mutants of junin virus.two temperature sensitive (ts) mutants of junin virus, a member of arenaviridae, have been partially characterized. both mutants, named ts-32 and ts-40, had a relative plating efficiency 40 degrees c/34 degrees c lower than 10(-3) an exhibited a leak yield below 10(-4). standard growth curves showed that at 34 degrees c the viral mutants multiplied slower than wt virus and at high multiplicity did not display autointerference. no differences in thermolability were observed between wt and ts muta ...19863018452
chronic neurologic disease in junín virus-infected rats.the purpose of this study was to determine whether junín virus persistence in cns of rats was capable of inducing late neurologic disease. following intracerebral inoculation of newborn animals with xj strain, three distinct stages could be discerned: an early phase of acute disease, up to 30 days pi, with 5% mortality; an intermediate one, extending to 280 days pi, without clinical signs but with evident viral persistence; and a final period of chronic illness, featuring clinical neurologic syn ...19863020168
infection of cebus monkeys with junín virus. 19853020338
[inactivated junin virus antigens]. 19853020339
[junin virus activity in humans and rodents of non-endemic areas of the province of buenos aires]. 19853020341
[effect of cyclophosphamide on the development of infection in calomys musculinus with junin virus]. 19853022105
[effect of cyclophosphamide on the development of infection in calomys musculinus with junin virus]. 19853022105
treatment of junin virus-infected guinea pigs with immune serum: development of late neurological disease.guinea pigs infected with argentine hemorrhagic fever virus (junin) were treated with pooled, homologous convalescent sera. use of 15,000 or 5,000 therapeutic units of immune sera prevented all signs of illness when administered within 24 hr of infection. we could also prevent illness and death in infected guinea pigs as late as 6 days after infection if we used more antisera (30,000 therapeutic units/kg). in some treatment groups, surviving animals developed a late neurological syndrome with pr ...19863023540
treatment of junin virus-infected guinea pigs with immune serum: development of late neurological disease.guinea pigs infected with argentine hemorrhagic fever virus (junin) were treated with pooled, homologous convalescent sera. use of 15,000 or 5,000 therapeutic units of immune sera prevented all signs of illness when administered within 24 hr of infection. we could also prevent illness and death in infected guinea pigs as late as 6 days after infection if we used more antisera (30,000 therapeutic units/kg). in some treatment groups, surviving animals developed a late neurological syndrome with pr ...19863023540
therapeutic effect of the antiviral agent ribavirin in junín virus infection of primates.in order to assess the effect of the antiviral ribavirin on the course of junín virus infection in callithrix jacchus, seven inoculated monkeys were treated with 15 mg of the drug, twice a day, starting 6 days after infection when all animals were viremic. the three untreated controls showed typical signs of junín virus infection at 14 days pi and their mean time of death was 18 days. in contrast, no signs of illness were detected in ribavirin-treated animals until 24 days pi, when marmosets sho ...19863023541
therapeutic effect of the antiviral agent ribavirin in junín virus infection of primates.in order to assess the effect of the antiviral ribavirin on the course of junín virus infection in callithrix jacchus, seven inoculated monkeys were treated with 15 mg of the drug, twice a day, starting 6 days after infection when all animals were viremic. the three untreated controls showed typical signs of junín virus infection at 14 days pi and their mean time of death was 18 days. in contrast, no signs of illness were detected in ribavirin-treated animals until 24 days pi, when marmosets sho ...19863023541
[mononuclear phagocytes in the resistance of mice to extraneural infection with junin virus]. 19853023788
[modification of the course of junin virus infection in guinea pigs by administration of immune sera]. 19853023791
[modification of the course of junin virus infection in guinea pigs by administration of immune sera]. 19853023791
protection of junín virus-infected marmosets by passive administration of immune serum: association with late neurologic signs.argentine hemorrhagic fever (junín virus) is a human viral disease for which immune therapy proves effective, though a late neurologic syndrome is occasionally associated with the treatment. we attempted to determine in the infected marmoset callithrix jacchus whether immune therapy leads to protection and/or cns damage. fifteen c jacchus were inoculated with 10(3) tissue culture infectious dose 50% (tcid50) of the xj strain of junín virus. on day 6 post infection (pi), 12 primates were treated ...19873025358
protection of junín virus-infected marmosets by passive administration of immune serum: association with late neurologic signs.argentine hemorrhagic fever (junín virus) is a human viral disease for which immune therapy proves effective, though a late neurologic syndrome is occasionally associated with the treatment. we attempted to determine in the infected marmoset callithrix jacchus whether immune therapy leads to protection and/or cns damage. fifteen c jacchus were inoculated with 10(3) tissue culture infectious dose 50% (tcid50) of the xj strain of junín virus. on day 6 post infection (pi), 12 primates were treated ...19873025358
experimental argentine hemorrhagic fever: myocardial involvement in cebus monkey.four cebus apella monkeys, to test a recently proposed model for testing neurovirulence of junín virus (jv) strains, were intracerebrally infected with 10(5) ld50 of the xj clone 3 strain of jv. there were no significant electrocardiographic abnormalities or gross lesions, but all infected monkeys exhibited a varying degree of histologic myocardial lesions including focal lymphoblastic infiltrates, vascular ruptures, and mild interstitial reactive change. one cebus showed lymphocytic infiltrates ...19863025446
experimental argentine hemorrhagic fever: myocardial involvement in cebus monkey.four cebus apella monkeys, to test a recently proposed model for testing neurovirulence of junín virus (jv) strains, were intracerebrally infected with 10(5) ld50 of the xj clone 3 strain of jv. there were no significant electrocardiographic abnormalities or gross lesions, but all infected monkeys exhibited a varying degree of histologic myocardial lesions including focal lymphoblastic infiltrates, vascular ruptures, and mild interstitial reactive change. one cebus showed lymphocytic infiltrates ...19863025446
induction of junin virus persistence in adult athymic mice.to determine the role of t lymphocytes in adult mice infected with junin virus, 60-day-old athymic (nu/nu) mice and their immunocompetent (nu/+) littermates were inoculated intracerebrally with 10(3) tcd50 of the xj strain. none of them exhibited neurologic illness during a 6-month observation period, and mortality was 3% for nu/nu and 7% for nu/+ animals. the main features in infected nu/nu mice were: high viral titers in brain, reaching a late peak (6.5 log/ml) 32 days postinoculation and pers ...19863026991
modification of junin virus neurotropism in mice by selective brain or spinal cord passaging.the percentage of suckling mice that developed paralysis after intracerebral junin virus (xj-jv pathogenic strain) inoculation (13.8%) consistently increased after 5 serial passages of virus-infected brain or spinal cord obtained from paralytic animals, reaching 37.9 and 45.7%, respectively. as expected, all paralytic mice exhibited an identical spinal cord histologic picture, with widespread jv antigen in spinal cord astrocytes and neurons, particularly the large motor neurons of the anterior h ...19863026992
in vitro induction of human polymorphonuclear leukocyte damage by junin virus. 19863027495
intracerebral infection of cebus apella with the xj-clone 3 strain of junín virus.to assess the usefulness of the south american primate cebus apella as a model for neurovirulence of junín virus, eight monkeys were inoculated with 10(5) ld50 of the attenuated xj-clone 3 junín virus strain by the intrathalamic route. after the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. moderate clinical central nervous system (cns) involvement was present in four of eight monkeys, while the rest had only ...19873031201
intracerebral infection of cebus apella with the xj-clone 3 strain of junín virus.to assess the usefulness of the south american primate cebus apella as a model for neurovirulence of junín virus, eight monkeys were inoculated with 10(5) ld50 of the attenuated xj-clone 3 junín virus strain by the intrathalamic route. after the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. moderate clinical central nervous system (cns) involvement was present in four of eight monkeys, while the rest had only ...19873031201
experimental argentine hemorrhagic fever in rhesus macaques: virus-specific variations in pathology.two isolates of junin virus (espindola and ledesma) inoculated into rhesus macaques produced distinct lesions which were strain-constant and similar to reported human cases of argentine hemorrhagic fever. the espindola isolate was associated with hemorrhagia, necrosis of bone marrow, and mild hepatocellular necrosis. ledesma isolate was associated with pronounced polioencephalomyelitis and autonomic ganglioneuritis, but very mild or absent hepatocellular necrosis, bone marrow necrosis, and hemor ...19873039051
experimental argentine hemorrhagic fever in rhesus macaques: virus-specific variations in pathology.two isolates of junin virus (espindola and ledesma) inoculated into rhesus macaques produced distinct lesions which were strain-constant and similar to reported human cases of argentine hemorrhagic fever. the espindola isolate was associated with hemorrhagia, necrosis of bone marrow, and mild hepatocellular necrosis. ledesma isolate was associated with pronounced polioencephalomyelitis and autonomic ganglioneuritis, but very mild or absent hepatocellular necrosis, bone marrow necrosis, and hemor ...19873039051
effect of polymorphonuclear depletion on experimental argentine hemorrhagic fever in guinea pigs.the role that polymorphonuclear leukocytes (pmn) may play in argentine hemorrhagic fever (ahf), an endemo-epidemic disease caused by junín virus (jv), was investigated in experimentally infected guinea pigs depleted of pmn by means of specific antiserum. in leucopenic animals the evolution of the infection with a highly pathogenic strain of jv was more severe, with earlier mortality and higher virus yields in blood and viscera. the pathological study showed similar lesions in both the control an ...19873040897
effect of polymorphonuclear depletion on experimental argentine hemorrhagic fever in guinea pigs.the role that polymorphonuclear leukocytes (pmn) may play in argentine hemorrhagic fever (ahf), an endemo-epidemic disease caused by junín virus (jv), was investigated in experimentally infected guinea pigs depleted of pmn by means of specific antiserum. in leucopenic animals the evolution of the infection with a highly pathogenic strain of jv was more severe, with earlier mortality and higher virus yields in blood and viscera. the pathological study showed similar lesions in both the control an ...19873040897
mouse splenocyte transfer effect depends on donor's junin virus infection stage.splenocytes from junin-virus-persistently-infected euthymic mice taken at 45 days postinfection seemed unable to induce overt signs of disease, to cause death, or to modify brain viral levels when transferred to athymic junin-virus-infected mice. findings differed sharply when the same recipients were transferred with splenocytes taken at 6 or 30 days postinfection from immunocompetent mice infected in adult life, since mortality reached 80 or 50%, respectively, and brain viral titers were signi ...19883260227
geographic patterns of allele frequencies in calomys musculinus reservoir-host of junin virus. 19863543610
geographic patterns of allele frequencies in calomys musculinus reservoir-host of junin virus. 19863543610
effect of actinomycin d on arenavirus growth and estimation of the generation time for a virus particle.in an attempt to define the involvement of host transcription in arenavirus growth, a study was made of the effect of actinomycin d (amd) on the yields of infectious pichinde, tacaribe and junin viruses. the drug was added either immediately after virus adsorption or later after infection, at the stationary phase of virus growth. the time of exposure of the infected cells to the inhibitor was so chosen that the generation and release of virus into the medium took place in the presence of amd. a ...19863765824
[in vivo inhibition of t lymphocyte dth in encephalitis produced by junin virus]. 19853879861
endotoxin-induced protection against experimental junín virus infection. 19734199435
immunosuppression by junin virus. ultrastructural study. 19744213176
junín virus. 19744213994
[antigenic relations among 7 strains of junin virus isolated during epidemics of several years]. 19674229417
[electrolyte modifications of the plasma of guinea pigs infected by junin virus]. 19684238205
[electrolyte modifications of the plasma of guinea pigs infected by junin virus]. 19684238205
[dimensions of the junin virus (argentine hemorrhagic fever)]. 19694241369
[changes in lipids in the blood of guinea pigs infected with junin virus]. 19694259111
[changes in lipids in the blood of guinea pigs infected with junin virus]. 19694259111
[purification of junin virus from the brain of infected mice]. 19694259112
[analysis of factors conditioning plaque formation in vero cells infected with tacaribe and junin virus (fha)]. 19744377464
nutritional requirements for junin virus production in bhk culture cells. 19744377723
phytohaemagglutinin unresponsiveness in leukopenia induced by junin virus in guinea pigs. 19724402253
phytohaemagglutinin unresponsiveness in leukopenia induced by junin virus in guinea pigs. 19724402253
ultrastructure of junín virus in mouse whole brain and mouse brain tissue cultures.comparative ultrastructural studies were performed on the development of junín virus in mouse brain and in cerebellum explants and brain monolayers of the same animal. in mouse brain, neurons and astrocytes released virus particles by a budding mechanism identical to that previously described for this virus. in the neurons, the viral multiplication took place in the perikarion as well as in the cytoplasmic processes, including areas near synapses. viral particles were observed emerging from peri ...19744417948
[protein synthesis and formation of junin virus in bhk cells]. 19744424508
the brains of suckling mice infected with junin virus; fractionation and study. 19724509283
[vero cells persistently infected with junin virus. i. behavior of the vero ir 58 cell line after superinfection with 2 strains of junin virus]. 19724559344
cytopathogenic process in junin virus infected vero cells. 19724626277
the occurrence of virus, interferon, and circulating antibodies in mice after experimental infection with junin virus. 19734632558
effect of cyclophosphamide on junin virus infection of mice. 19734755520
[study of the experimental infection of mice by junin virus. effect of antithymocyte serum]. 19734755522
junín virus encephalitis in mice: its inhibition by antithymocyte serum. 19734772936
[virus-host interrelation in experimental infection of mice with junin virus and other related viruses]. 19734798427
pathogenesis of junin virus infection in the infant hamster. 19684913569
tacaribe virus infection of guinea-pig. virus distribution, appearance of antibodies and immunity against junin virus infection. 19674970562
tacaribe virus infection of guinea-pig. virus distribution, appearance of antibodies and immunity against junin virus infection. 19674970562
[purification of junin virus (argentinian hemorrhagic fever) from the brain of infected mice]. 19684982868
the effect of 5-iododeoxyuridine and actinomycin d on the multiplication of junin virus. 19694984060
[interaction of junin virus with monkey kidney cells]. 19704994071
[presence of an inhibitor factor of junin virus in the brain of the normal adult rat]. 19704994072
[presence of an inhibitor factor of junin virus in the brain of the normal adult rat]. 19705005646
[interaction of junin virus with monkey kidney cells]. 19705005812
susceptibility of junin virus to amantadine hydrochloride. 19725019632
[comparative study of the effect of junin virus in different cellular cultures]. 19725051392
biological properties of junin virus purified from infected mouse brain. 19725051393
[susceptibility of a hela-rat cell mixed culture to 2 junin virus strains]. 19715099342
[immunological studies in experimental argentinian hemorrhagic fever. i. effect of an attenuated strain of junin virus in the immunological response]. 19715107331
ii. attempts to grow tacaribe and junin viruses in grace's continuous line of moth cells. 19715142325
[leukocyte alterations produced in guinea pigs by 2 strains of junin virus]. 19715162332
[leukocyte alterations produced in guinea pigs by 2 strains of junin virus]. 19715162332
[pathogenesis of encephalitis caused by junin virus: studies in newborn mice]. 19715162333
[hematologic studies in guinea pigs infected with junin virus xj and xj, c13 strains]. 19695345414
[hematologic studies in guinea pigs infected with junin virus xj and xj, c13 strains]. 19695345414
[immunization against argentinian hemorrhagic fever using an attenuated strain of junin virus. i. modified strain of junin virus. immunization of guinea pigs]. 19695346952
[immunization against argentinian hemorrhagic fever using an attenuated strain of junin virus. i. modified strain of junin virus. immunization of guinea pigs]. 19695346952
[electron microscopy of primary cultures of mouse fibroblasts inoculated with junin virus]. 19695406455
[inhibition of the secondary immune response in experimental hemorrhagic fever (junin virus)]. 19705453086
depression of "jones mote" type hypersensitivity by junin virus. 19705457235
susceptibility of mice aged 0-14 days to infection with junin virus. 19705457731
alterations in the enzymatic activity of plasma of guinea pigs infected with junín virus. 19705460528
alterations in the enzymatic activity of plasma of guinea pigs infected with junín virus. 19705460528
[evaluation of a method of purification of junin virus]. 19705504267
[a simple method of obtaining a complement fixation antigen of junin virus]. 19705504268
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