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triplin, a small molecule, reveals copper ion transport in ethylene signaling from atx1 to ran1.copper ions play an important role in ethylene receptor biogenesis and proper function. the copper transporter responsive-to-antagonist1 (ran1) is essential for copper ion transport in arabidopsis thaliana. however it is still unclear how copper ions are delivered to ran1 and how copper ions affect ethylene receptors. there is not a specific copper chelator which could be used to explore these questions. here, by chemical genetics, we identified a novel small molecule, triplin, which could cause ...201728388654
genome-wide identification and expression analysis of cllax, clpin and clabcb genes families in citrullus lanatus under various abiotic stresses and grafting.auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. auxin is transported by three kinds of major protein families, including the auxin resistant 1/like aux1 (aux⁄lax) influx carriers, the pin-formed (pin) efflux carriers and the atp binding cassette b/p-glycoprotein/multidrug-resistance (abcb/mdr/pgp) efflux/condition carriers. the biological function of several auxin transporter genes has been well characterized in a ...201728388893
acetolactate synthase regulatory subunits play divergent and overlapping roles in branched-chain amino acid synthesis and arabidopsis development.branched-chain amino acids (bcaas) are synthesized by plants, fungi, bacteria, and archaea with plants being the major source of these amino acids in animal diets. acetolactate synthase (als) is the first enzyme in the bcaa synthesis pathway. although the functional contribution of als to bcaa biosynthesis has been extensively characterized, a comprehensive understanding of the regulation of this pathway at the molecular level is still lacking.201728388946
characterization and functional analysis of four hyh splicing variants in arabidopsis hypocotyl elongation.arabidopsis thaliana long hypocotyl5 (hy5) is a positive regulator of the light signaling pathway. the hy5 mutant has an elongated hypocotyl in all light conditions, whereas the hy5 homolog (hyh) mutant has a very weak phenotype, but only in blue light. however, overexpression of hyh rescues the elongated hypocotyl phenotype in the hy5 null mutant. here, we report the identification of four hyh splicing variants in arabidopsis. alternative splicing in the 5' region of the hyh gene occurred such ...201728389360
a two-step model for de novo activation of wuschel during plant shoot regeneration.plant cells are totipotent and competent to regenerate from differentiated organs. it has been known for six decades that cytokinin-rich medium induces shoot regeneration from callus cells. however, the underlying molecular mechanism remains elusive. the homeodomain transcription factor wuschel (wus) is essential for de novo establishment of the shoot stem cell niche in arabidopsis thaliana. we found that wus-positive cells (wus+) mark the shoot progenitor region during regeneration. a cytokinin ...201728389585
lmi1-like genes involved in leaf margin development of brassica napus.in rapeseed (brassica napus l.), leaf margins are variable and can be entire, serrate, or lobed. in our previous study, the lobed-leaf gene (lobed-leaf 1, bnll1) was mapped to a 32.1 kb section of b. napus a10. two lmi1-like genes, bnaa10g26320d and bnaa10g26330d, were considered the potential genes that controlled the lobed-leaf trait in rapeseed. in the present study, these two genes and another homologous gene (bnac04g00850d) were transformed into arabidopsis thaliana (l.) heynh. plants to id ...201728389974
sugar and hexokinase suppress expression of pip aquaporins and reduce leaf hydraulics that preserves leaf water potential.sugars affect central aspects of plant physiology, including photosynthesis, stomatal behavior and the loss of water through the stomata. yet, the potential effects of sugars on plant aquaporins (aqps) and water conductance have not been examined. we used database and transcriptional analyses, as well as cellular and whole-plant functional techniques to examine the link between sugar-related genes and aqps. database analyses revealed a high level of correlation between the expression of aqps and ...201728390076
asn1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in arabidopsis seeds.despite a general view that asparagine synthetase generates asparagine as a long-distance nitrogen transport amino acid to sink organs, its role in nitrogen metabolic pathways in floral organs during seed nitrogen filling has remained undefined. we demonstrate that onset of pollination in arabidopsis induces selected genes of asparagine metabolism particularly at ovule stage (stage 0): asn1 (at3g47340), gln2 (at5g35630), glu1 (at5g04140), aapat2 (at5g19950), aspga1 (at5g08100) and aspgb1 (at3g16 ...201728390103
involvement of salicylic acid, ethylene and jasmonate acid signaling pathways in susceptibility of tomato for fusarium oxysporum.phytohormones such as salicylic acid (sa), ethylene (et) and jasmonic acid (ja) play key roles in plant defense following pathogen attack. involvement of these hormones in susceptibility following fusarium oxysporum (fo) infection has mostly been studied in arabidopsis thaliana. fo, however, causes vascular wilt disease in a broad range of crops, including tomato (solanum lycopersicum). surprisingly little is known about the involvement of these phytohormones in susceptibility of tomato towards ...201728390170
ethylene response factor aterf72 negatively regulates arabidopsis thaliana response to iron deficiency.ethylene regulates the plant's response to stress caused by iron (fe) deficiency. however, specific roles of erf proteins in response to fe deficiency remain poorly understood. here, we investigated the role of erf72 in response to iron deficiency in arabidopsis thaliana. in this study, the levels of the ethylene response factor aterf72 increased in leaves and roots induced under the iron deficient conditions. erf72 mutant plants showed increased growth compared to wild type (wt) when grown in i ...201728390898
proteomic analysis of arabidopsis plasma membranes reveals lipopolysaccharide-responsive changes.plant plasma membranes (pms) contain pattern recognition receptors (prrs), lately believed to be associated within multicomponent complexes, which perceive microbe-associated molecular pattern (mamp) molecules like lipopolysaccharides (lpss) and result in signal transduction events that lead to activated immune defense responses. as such, arabidopsis thaliana leaves were treated with lps from escherichia coli (lpse.coli) over time, and pm fractions isolated and evaluated using gel-based and subs ...201728390899
molecular, structural, and phylogenetic analyses of taxus chinensis jazs.taxus spp. are ancient gymnosperms that produce a unique secondary metabolite, namely, taxol, an anticancer drug. jaz proteins are key regulators of the ja signaling pathway, which control taxol biosynthesis. however, the jaz proteins of taxus spp. are poorly studied. in this work, nine jaz genes from taxus chinensis were identified using our previous transcriptome data and named as tcjaz1-tcjaz9. of these nine tcjaz proteins, eight contain jas and tify domains, and the jas domain of tcjaz6 is i ...201728390989
arabidopsis abi5 plays a role in regulating ros homeostasis by activating catalase 1 transcription in seed germination.it has been known that aba insensitive 5 (abi5) plays a vital role in regulating seed germination. in the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-at) inhibits seed germination of col-0, abi5 mutants and abi5-overexpression transgenic lines. compared with col-0, the seeds of abi5 mutants showed more sensitive to 3-at during seed germination, while the seeds of abi5-overexpression transgenic lines showed more insensitive. h2o2 showed the sam ...201728391398
strigolactone- and karrikin-independent smxl proteins are central regulators of phloem formation.plant stem cell niches, the meristems, require long-distance transport of energy metabolites and signaling molecules along the phloem tissue. however, currently it is unclear how specification of phloem cells is controlled. here we show that the genes suppressor of max2 1-like3 (smxl3), smxl4, and smxl5 act as cell-autonomous key regulators of phloem formation in arabidopsis thaliana. the three genes form an uncharacterized subclade of the smxl gene family that mediates hormonal strigolactone an ...201728392107
phenylalanine ammonia lyase from arabidopsis thaliana (atpal2): a potent enzyme for the synthesis of non-canonical aromatic alpha-amino acids: part i: comparative characterization to the enzymes from petroselinum crispum (pcpal1) and rhodosporidium toruloides (rtpal).phenylalanine ammonia lyase (pal) from arabidopsis thaliana (atpal2) was comparatively characterized to the well-studied enzyme from parsley (pcpal1) and rhodosporidium toruloides (rtpal) with respect to kinetic parameters for the deamination and the amination reaction, ph- and temperature optima and the substrate range of the amination reaction. whereas both plant enzymes are specific for phenylalanine, the bifunctional enzyme from rhodosporidium toruloides shows km-values for l-phe and l-tyr i ...201728392421
the heterologous expression of a chrysanthemum tcp-p transcription factor cmtcp14 suppresses organ size and delays senescence in arabidopsis thaliana.tcp transcription factors are important for plant growth and development, but their activity in chrysanthemum (chrysanthemum morifolium) has not been thoroughly explored. here, a chrysanthemum tcp-p sequence, which encodes a protein harboring the conserved basic helix-loop-helix (bhlh) motif, was shown to be related phylogenetically to the arabidopsis thaliana gene attcp14. a yeast-one hybrid assay showed that the encoding protein had no transcriptional activation ability, and a localization exp ...201728395169
pif4-controlled auxin pathway contributes to hybrid vigor in arabidopsis thaliana.f1 hybrids in arabidopsis and crop species are uniform and high yielding. the f2 generation loses much of the yield advantage and the plants have heterogeneous phenotypes. we generated pure breeding hybrid mimic lines by recurrent selection and also selected a pure breeding small phenotype line. the hybrid mimics are almost completely homozygous with chromosome segments from each parent. four particular chromosomal segments from c24 and 8 from ler were present in all of the hybrid mimic lines, w ...201728396418
engineering plant membranes using droplet interface bilayers.droplet interface bilayers (dibs) have become widely recognised as a robust platform for constructing model membranes and are emerging as a key technology for the bottom-up assembly of synthetic cell-like and tissue-like structures. dibs are formed when lipid-monolayer coated water droplets are brought together inside a well of oil, which is excluded from the interface as the dib forms. the unique features of the system, compared to traditional approaches (e.g., supported lipid bilayers, black l ...201728396711
transcriptomic response of arabidopsis thaliana (l.) heynh. roots to ibuprofen.surface waters in urban areas are contaminated by ibuprofen (ibp), a popular and extensively used anti-inflammatory drug. in this study, we investigated the transcriptomic response in arabidopsis thaliana (l.) heynh. roots with the aim of revealing genes that are potentially involved in ibp detoxification and to elucidate the effect of ibp on plants. ibp up-regulated 63 and down-regulated 38 transcripts (p-value < 0.1, fold change ≥ 2) after two-day exposure to a 5 µm (1.03 mg l(-1)) concentrati ...201728398082
sinat e3 ligases control the light-mediated stability of the brassinosteroid-activated transcription factor bes1 in arabidopsis.the plant hormones brassinosteroids (brs) participate in light-mediated regulation of plant growth, although the underlying mechanisms are far from being fully understood. in addition, the function of the core transcription factor in the br signaling pathway, bri1-ems-suppressor 1 (bes1), largely depends on its phosphorylation status and its protein stability, but the regulation of bes1 is not well understood. here, we report that sina of arabidopsis thaliana (sinats) specifically interact with ...201728399399
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