Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| distinct african swine fever virus shedding in wild boar infected with virulent and attenuated isolates. | since the reappearance of african swine fever virus (asfv), the disease has spread in an unprecedented animal pandemic in eurasia. asf currently constitutes the greatest global problem for the swine industry. the wild boar (sus scrofa) in which the pathogen has established wild self-sustaining cycles, is a key reservoir for asfv, signifying that there is an urgent need to develop an effective vaccine against this virus. current scientific debate addresses whether live attenuated vaccines (lavs), ... | 2020 | 33339147 |
| detection of african swine fever virus in free-ranging wild boar in southeast asia. | african swine fever (asf) is a highly contagious and fatal viral disease affecting both domestic and wild suids. the virus was introduced to southeast asia in early 2019 and has since spread rapidly throughout the region. although significant efforts have been made to track and diagnose the disease in domestic pigs, very little is known about asf in free-ranging wild boar and their potential role in maintaining the disease within southeast asia. through a collaboration between government and non ... | 2020 | 33351995 |
| african swine fever virus structural protein p17 inhibits cell proliferation through er stress-ros mediated cell cycle arrest. | african swine fever virus (asfv) is a highly pathogenic large dna virus that causes african swine fever (asf) in domestic pigs and wild boars. the p17 protein, encoded by the d117l gene, is a major transmembrane protein of the capsid and the inner lipid envelope. the aim of this study was to investigate the effects of p17 on cell proliferation and the underlying mechanisms of action. the effects of p17 on cell proliferation, cell cycle, apoptosis, oxidative stress, and endoplasmic reticulum (er) ... | 2020 | 33374251 |
| unpicking the secrets of african swine fever viral replication sites. | african swine fever virus (asfv) is a highly contagious pathogen which causes a lethal haemorrhagic fever in domestic pigs and wild boar. the large, double-stranded dna virus replicates in perinuclear cytoplasmic replication sites known as viral factories. these factories are complex, multi-dimensional structures. here we investigated the protein and membrane compartments of the factory using super-resolution and electron tomography. click it chemistry in combination with stimulated emission dep ... | 2021 | 33429879 |
| molecular profile of african swine fever virus (asfv) circulating in vietnam during 2019-2020 outbreaks. | african swine fever (asf) is a highly infectious disease of pigs caused by african swine fever virus (asfv). in order to identify potential genetic variations among asfv strains circulating in vietnam, 26 asfv isolates from organs and blood samples collected from domestic pigs from 23 different provinces of northern, central and southern vietnam during 2019-2020 asf outbreaks were genetically characterized. nucleotide sequences were determined for a portion of the b646l (p72) gene, the complete ... | 2021 | 33454861 |
| social structure defines spatial transmission of african swine fever in wild boar. | the spatial spread of infectious disease is determined by spatial and social processes such as animal space use and family group structure. yet, the impacts of social processes on spatial spread remain poorly understood and estimates of spatial transmission kernels (stks) often exclude social structure. understanding the impacts of social structure on stks is important for obtaining robust inferences for policy decisions and optimizing response plans. we fit spatially explicit transmission model ... | 2021 | 33468025 |
| no evidence for african swine fever virus dna in haematophagous arthropods collected at wild boar baiting sites in estonia. | african swine fever (asf) is a highly pathogenic viral disease affecting all suidae, with ornithodoros moubata complex soft ticks acting as the biological arthropod vectors of the causative agent, african swine fever virus (asfv). while asfv is also transmissible via direct contact, pig products and fomites, other arthropods may be involved in virus transmission and persistence. therefore, we checked various groups of blood-feeding arthropods collected during summer 2017 in wild boar habitats on ... | 2021 | 33527715 |
| self-amplifying pestivirus replicon rna encoding influenza virus nucleoprotein and hemagglutinin promote humoral and cellular immune responses in pigs. | self-amplifying replicon rna (reprna) promotes expansion of mrna templates encoding genes of interest through their replicative nature, thus providing increased antigen payloads. reprna derived from the non-cytopathogenic classical swine fever virus (csfv) targets monocytes and dendritic cells (dcs), potentially promoting prolonged antigen expression in the dcs, contrasting with cytopathogenic reprna. we engineered pestivirus reprna constructs encoding influenza virus h5n1 (a/chicken/yamaguchi/7 ... | 2021 | 33584723 |
| african swine fever spread across asia, 2018-2019. | african swine fever virus (asfv) is a highly contagious pathogen that causes disease in pigs, commonly characterized by acute haemorrhagic fever. prior to august 2018, african swine fever (asf) had not been reported in asia, but has since spread throughout china, mongolia, korea, vietnam, laos, cambodia, myanmar, the philippines, hong kong, indonesia, timor-leste and papua new guinea. using data collated from reports of confirmed cases, we applied spatio-temporal analysis to describe asfv spread ... | 2021 | 33599077 |
| t-cell responses in domestic pigs and wild boar upon infection with the moderately virulent african swine fever virus strain 'estonia2014'. | infection with african swine fever virus (asfv) causes a highly lethal haemorrhagic disease in domestic and eurasian wild pigs. thus, it is a major threat to pig populations worldwide and a cause of substantial economic losses. recently, less virulent asfv strains emerged naturally, which showed higher experimental virulence in wild boar than in domestic pigs. the reason for this difference in disease progression and outcome is unclear but likely involves different immunological responses. unfor ... | 2021 | 33630409 |
| antibody prevalence to african swine fever virus, mycobacterium bovis, foot-and-mouth disease virus, rift valley fever virus, influenza a virus, and brucella and leptospira spp. in free-ranging warthog (phacochoerus africanus) populations in south africa. | the warthog (phacochoerus africanus) can be used as a model for investigating disease transmission at the human, wildlife, and livestock interface. an omnivore and scavenger, a warthog moves freely between natural ecotypes, farmland, and human communities and is susceptible to diseases of zoonotic, agricultural, and conservation concern. a retrospective study using 100 individual serum samples collected from may 1999 to august 2016 was performed to determine antibody prevalence to seven pathogen ... | 2021 | 33635986 |
| an immortalized porcine macrophage cell line competent for the isolation of african swine fever virus. | african swine fever virus (asfv) is the etiological agent of african swine fever (asf), a fatal hemorrhagic disease of domestic pigs and wild boar. the virus primarily infects macrophage and monocyte host cells, these do not grow in vitro. many attempts have been made to establish sustainable asfv-sensitive cell lines, but which supported only low viral replication levels of limited, mostly artificially attenuated strains of asfv. here, we examined the competence of a novel cell line of immortal ... | 2021 | 33637799 |
| swift and reliable easy lab methods for the sensitive molecular detection of african swine fever virus. | african swine fever (asf) is a contagious viral hemorrhagic disease of domestic pigs and wild boars. the disease is notifiable to the world organisation for animal health (oie) and is responsible for high mortality and serious economic losses. pcr and real-time pcr (qpcr) are the oie-recommended standard methods for the direct detection of african swine fever virus (asfv) dna. the aim of our work was the simplification and standardization of the molecular diagnostic workflow in the lab. for vali ... | 2021 | 33669073 |
| efficacy of oral vaccine against classical swine fever in wild boar and estimation of the disease dynamics in the quantitative approach. | classical swine fever virus (csfv) in the wild boar population has been spreading in japan, alongside outbreaks on pigs, since classical swine fever (csf) reemerged in september 2018. the vaccination using oral bait vaccine was initially implemented in gifu prefecture in march 2019. in the present study, antibodies against csfv in wild boar were assessed in 1443 captured and dead wild boars in gifu prefecture. after the implementation of oral vaccination, the increase of the proportion of seropo ... | 2021 | 33672749 |
| african swine fever virus mgf-110-9l-deficient mutant has attenuated virulence in pigs. | african swine fever virus (asfv) is the etiological agent of african swine fever (asf), an often lethal disease in domestic and wild pigs. asf represents a major threat to the swine industry worldwide. currently, no commercial vaccine is available because of the complexity of asfv or biosecurity concerns. live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous asfv strain challenge. in the present study, a mutant asf ... | 2021 | 33689140 |
| asf exit strategy: providing cumulative evidence of the absence of african swine fever virus circulation in wild boar populations using standard surveillance measures. | efsa assessed the role of seropositive wild boar in african swine fever (asf) persistence. surveillance data from estonia and latvia investigated with a generalised equation method demonstrated a significantly slower decline in seroprevalence in adult animals compared with subadults. the seroprevalence in adults, taking more than 24 months to approach zero after the last detection of asfv circulation, would be a poor indicator to demonstrate the absence of virus circulation. a narrative literatu ... | 2021 | 33717352 |
| subgrouping and analysis of relationships between classical swine fever virus identified during the 2018-2020 epidemic in japan by a novel approach using shared genomic variants. | classical swine fever (csf) is a worldwide devastating disease of the pig industry caused by classical swine fever virus (csfv). in september 2018, an outbreak of csf occurred in japan where the disease had been eradicated and was officially designated a csf-free country since 2015. following the detection of the first 2018 case on a farm in gifu prefecture, the disease spread among both farm pigs and wild boars and still continues. epigenome analysis using whole-genome information is helpful in ... | 2021 | 33730417 |
| identification of african swine fever virus genomic dnas in wild boar habitats within outbreak regions in south korea. | an african swine fever (asf) outbreak in wild boars was first reported on october 2, 2019, in south korea. since then, additional cases were reported in south korea's border areas. we here report the identification of asf virus (asfv) dnas from two out of eight environmental abiotic matter samples collected from areas where asf-positive wild boar carcasses were found. comparative genomic investigations suggested that the contaminating asfv dnas originated from the wild boar whose carcass had bee ... | 2021 | 33774943 |
| the potential negative impacts of the classical swine fever virus on wild boar population in gifu prefecture, japan. | there is a possibility that classical swine fever (csf) virus outbreak has negative impacts on wild boar. to adequately manage native wild boar populations, wildlife managers need to gather the field data on wild boar and implement population management practices. we aimed to report the relative abundance index of wild boar before and after this outbreak. our results showed that relative abundance index declined from 2017 (8.88 wild boars/100 trap days) to 2019 (2.03 wild boars/100 trap days), b ... | 2021 | 33775990 |
| african swine fever virus genotype ii in mongolia, 2019. | african swine fever (asf) is a severe haemorrhagic disease of domestic and wild pigs caused by the african swine fever virus (asfv). in recent years, asf has steadily spread towards new geographical areas, reaching europe and asia. on january 15th, 2019, mongolia reported its first asf outbreak to the world organization for animal health (oie), becoming, after china, the second country in the region affected by the disease. following an event of unusual mortality in domestic pigs in bulgan provi ... | 2021 | 33818903 |
| rapid detection of african swine fever virus using cas12a-based portable paper diagnostics. | african swine fever virus (asfv) is a dsdna virus responsible for a severe, highly contagious, and lethal disease affecting both domestic and wild pigs. asfv has brought enormous economic loss to a number of countries, and effective vaccine and therapy are still lacking. therefore, a rapid, sensitive, and field-deployable detection of asfv is important for disease surveillance and control. herein, we developed a cas12a-mediated portable paper assay to rapidly and precisely detect asfv. we identi ... | 2020 | 33824296 |
| detection and quantification of african swine fever virus in ma-104 cells. | detection of live african swine fever virus (asfv) has historically relied on the use of primary swine macrophages (psm). psm do not replicate and have to be isolated fresh from donor swine. we previously identified that a ma-104 cells (atcc #crl-2378.1), a commercially available cell line isolated from african green monkey ( cercopithecus aethiops ) kidney epithelial cells, supports the detection of asfv from field samples with a sensitivity comparable to that of primary swine macrophages. coll ... | 2021 | 33855107 |
| phylogenetic and phylodynamic analysis of a classical swine fever virus outbreak in japan (2018-2020). | after 26 years, another classical swine fever virus (csfv) outbreak in domestic pigs and wild boars occurred in japan 2018. herein, we investigated the entry and the spatial dynamics of the csfv outbreak in japan using the nearly complete genomes of strains isolated from both wild boars and domestic pigs during this epidemic. phylogenetic analysis showed that the most recent common ancestor (mrca) of the japanese lineage emerged 146 days (95% highest posterior density (hpd): 85-216 days) before ... | 2021 | 33890426 |
| african swine fever in cameroon: a review. | african swine fever (asf) is a hemorrhagic contagious porcine disease caused by the african swine fever virus. the disease poses enormous problems to the pork industry with pig mortality ranging from 30% to 100%, depending on the virulence of the virus circulating. cameroon, situated in central africa is one of the countries in which the african swine fever virus (asfv) has been endemic since its first outbreak in 1982. the disease is a major problem to the pig industry causing huge economic los ... | 2021 | 33916101 |
| modelling the spatial distribution of asf-positive wild boar carcasses in south korea using 2019-2020 national surveillance data. | in september 2019, african swine fever (asf) was reported in south korea for the first time. since then, more than 651 asf cases in wild boars and 14 farm outbreaks have been notified in the country. despite the efforts to eradicate asf among wild boar populations, the number of reported asf-positive wild boar carcasses have increased recently. the purpose of this study was to characterize the spatial distribution of asf-positive wild boar carcasses to identify the risk factors associated with t ... | 2021 | 33922261 |
| asfv-g-∆i177l as an effective oral nasal vaccine against the eurasia strain of africa swine fever. | the african swine fever virus (asfv) is currently causing a pandemic affecting wild and domestic swine from western europe to asia. no commercial vaccines are available to prevent african swine fever (asf), resulting in overwhelming economic losses to the swine industry. we recently developed a recombinant vaccine candidate, asfvg-δi177l, by deleting the i177l gene from the genome of the highly virulent asfv strain georgia (asfv-g). asfv-g-δi177l has been proven safe and highly efficacious in ch ... | 2021 | 33925435 |
| comparative analysis of full genome sequences of african swine fever virus isolates taken from wild boars in russia in 2019. | in this study, we report on the full genome phylogenetic analysis of four asfv isolates obtained from wild boars in russia. these samples originated from two eastern and two western regions of russia in 2019. phylogenetic analysis indicated that the isolates were assigned to genotype ii and grouped according to their geographical origins. the two eastern isolates shared 99.99% sequence identity with isolates from china, poland, belgium, and moldova, whereas the western isolates had 99.98% sequen ... | 2021 | 33925986 |
| identification of a new cell-penetrating peptide derived from the african swine fever virus cd2v protein. | the african swine fever virus (asfv) is a huge and complex dna virus that can lead to the acute death of pigs and cause huge losses to the global swine industry. the cd2v protein is a transmembrane protein encoded by the asfv's ep402r gene, which can effectively inhibit the bystander lymphocyte proliferation in response to mitogens and mediate the absorption of red blood cells to asfv-infected cells. the cd2v protein contains repetitive amino acid sequences ([kpcppp]3 labeled as raas), which is ... | 2021 | 34006158 |
| comparative analysis of swine leukocyte antigen gene diversity in european farmed pigs. | in europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. the need for typing of the swine leukocyte antigen (sla) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments and their use in infection studies and for design of veterinary vaccines. in this study, we characterised the sla class i ... | 2021 | 34028065 |
| genome variability of classical swine fever virus during the 2018-2020 epidemic in japan. | although rna viruses exhibit extensive sequence diversity, the mutation rate must be limited to ensure protein functions that maintain the viral life cycle. here, we compared the whole genome sequences of 150 isolates of classical swine fever virus (csfv), obtained from a single epidemic that occurred in japan during 2018-2020. after the detection of the first case, the disease spread among both farm pigs and wild boars and caused severe impact on the pig industry. to evaluate the diversificatio ... | 2021 | 34058522 |
| research progress on live attenuated vaccine against african swine fever virus. | african swine fever (asf) is an acute, hemorrhagic and severe infectious disease caused by african swine fever virus (asfv) in domestic pigs and various wild boars, with a mortality rate up to 100%. asf was first discovered in 1921 in kenya. asfv has a large genome and complex immune escape mechanism creating difficulties in the production of vaccines. recently, remarkable advances have been made in vaccine development all over the world especially in live-attenuated vaccine. this article aims t ... | 2021 | 34089790 |
| the impact of african swine fever virus on smallholder village pig production: an outbreak investigation in lao pdr. | african swine fever virus (asfv) causes a deadly disease of pigs which spread through southeast asia in 2019. we investigated one of the first outbreaks of asfv in lao people's democratic republic amongst smallholder villages of thapangtong district, savannakhet province. in this study, two asfv affected villages were compared to two unaffected villages. evidence of asfv-like clinical signs appeared in pig herds as early as may 2019, with median epidemic days on 1 and 18 june in the two villages ... | 2021 | 34146447 |
| transmission dynamics of african swine fever virus, south korea, 2019. | african swine fever (asf) is a substantial concern for global food production and security. however, lack of epidemiologic data in affected areas has limited the knowledge of the main drivers of asf virus (asfv) transmission. to assess the role of vehicle movements and wild boar populations in spreading asfv to pig farms in south korea, we combined data generated by asf surveillance on pig farms and of wild boars with nationwide global positioning system-based tracking data for vehicles involved ... | 2021 | 34152953 |
| research objectives to fill knowledge gaps in african swine fever virus survival in the environment and carcasses, which could improve the control of african swine fever virus in wild boar populations. | the european commission requested that efsa provide study designs for the investigation of four research domains according to major gaps in knowledge identified by efsa in a report published in 2019: i) the patterns of seasonality of african swine fever (asf) in wild boar and domestic pigs in the eu; ii) the epidemiology of asf in wild boar; iii) survival of asf virus (asfv) in the environment and iv) transmission of asfv by vectors. in this scientific opinion, the third research domain on asfv ... | 2021 | 34188717 |
| research priorities to fill knowledge gaps in the control of african swine fever: possible transmission of african swine fever virus by vectors. | the european commission requested that efsa provide study designs for the investigation of four research domains according to major gaps in knowledge identified by efsa in a report published in 2019: (i) the patterns of seasonality of african swine fever (asf) in wild boar and domestic pigs in the eu; (ii) the epidemiology of asf in wild boar; (iii) survival of asf virus (asfv) in the environment and (iv) transmission of asfv by vectors. in this scientific opinion, the fourth research domain on ... | 2021 | 34188718 |
| african swine fever virus as a difficult opponent in the fight for a vaccine-current data. | prevention and control of african swine fever virus (asfv) in europe, asia, and africa seem to be extremely difficult in view of the ease with which it spreads, its high resistance to environmental conditions, and the many obstacles related to the introduction of effective specific immunoprophylaxis. biological properties of asfv indicate that the african swine fever (asf) pandemic will continue to develop and that only the implementation of an effective and safe vaccine will ensure a reduction ... | 2021 | 34201761 |
| the role of interleukine-10 and interferon-γ as potential markers of the evolution of african swine fever virus infection in wild boar. | african swine fever virus (asfv) is one of the most challenging pathogens to affect both domestic and wild pigs. the disease has now spread to europe and asia, causing great damage to the pig industry. although no commercial vaccine with which to control the disease is, as yet, available, some potential vaccine candidates have shown good results in terms of protection. however, little is known about the host immune mechanisms underlying that protection, especially in wild boar, which is the main ... | 2021 | 34203976 |
| evaluation of lesions and viral antigen distribution in domestic pigs inoculated intranasally with african swine fever virus ken05/tk1 (genotype x). | the understanding of the pathogenic mechanisms and the clinicopathological forms caused by currently circulating african swine fever virus (asfv) isolates is incomplete. so far, most of the studies have been focused on isolates classified within genotypes i and ii, the only genotypes that have circulated outside of africa. however, less is known about the clinical presentations and lesions induced by isolates belonging to the other twenty-two genotypes. therefore, the early clinicopathological i ... | 2021 | 34207265 |
| a semi-automated system of luciferase immunoprecipitation assay for rapid and easy detection of african swine fever virus antibody. | african swine fever (asf) is a highly contagious viral disease of domestic pigs and wild boars. for the disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (mb-lips) to detect asf virus (asfv) antibody. the mb-lips is based on magnetic beads modified with protein a/g and the recombinant fusion protein of asfv p30 and luciferase, where p30 functioned as the recognition element and the luciferase as the signal component. incubation and washing could ... | 2021 | 34260273 |
| rapid and sensitive rpa-cas12a-fluorescence assay for point-of-care detection of african swine fever virus. | african swine fever (asf) is a serious contagious disease that causes fatal haemorrhagic fever in domestic and wild pigs, with high morbidity. it has caused devastating damage to the swine industry worldwide, necessitating the focus of attention on detection of the asf pathogen, the african swine fever virus (asfv). in order to overcome the disadvantages of conventional diagnostic methods (e.g. time-consuming, demanding and unintuitive), quick detection tools with higher sensitivity need to be e ... | 2021 | 34280234 |
| lateral flow assays for the detection of african swine fever virus antigen are not fit for field diagnosis of wild boar carcasses. | african swine fever (asf) is one of the most important viral diseases of domestic pigs and wild boar. apart from endemic cycles in africa, asf is now continuously spreading in europe and asia. as asf leads to severe but unspecific clinical signs and high lethality, early pathogen detection is of utmost importance. recently, 'point-of-care' (poc) tests, especially immunochromatographic assays, have been intensively discussed for the use in remote areas but also in the context of on-farm epidemiol ... | 2021 | 34312995 |
| research priorities to fill knowledge gaps in wild boar management measures that could improve the control of african swine fever in wild boar populations. | the european commission asked efsa to provide study designs for the investigation of four research domains (rds) according to major gaps in knowledge identified by efsa in a report published in 2019: (rd 1) african swine fever (asf) epidemiology in wild boar; (rd 2) asf transmission by vectors; (rd 3) african swine fever virus (asfv) survival in the environment, and (rd 4) the patterns of seasonality of asf in wild boar and domestic pigs in the eu. in this scientific opinion, the second rd on as ... | 2021 | 34354769 |