Publications

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experimental inoculation of bacteria-free trichomonas vaginalis into human vaginae and its effect on the glycogen content of vaginal epithelia. 195514361903
[comments on trichomonas vaginalis]. 195414366444
infections of the vagina and cervix; comprehensive therapy for trichomonas vaginalis, monilia albicans and other non-specific infections. 195514382900
aldolase in trichomonas vaginalis. 195514391333
trichomonas vaginalis infection; evaluation of the susceptibility to systemic medication. 195514393235
transmission of trichomonas vaginalis in the eye of animals. 195514395270
[simple method for determining the presence of trichomonas vaginalis, by smear]. 195514396518
[pathogenic role of trichomonas vaginalis in diseases of the urinary tract determined by means of urinary analysis]. 196014399493
[diagnostic value of the determination of trichomonas vaginalis in urine]. 196014399494
trichomonas vaginalis cervicitis and its relationship to cervical cancer. a histocytological study. 195914411232
metabolic studies and chemotherapy of trichomonas vaginalis. 196014412830
[on the usefulness of cultures in the study of trichomonas vaginalis infections]. 195914413636
a comparative study of the subcutaneous inoculation of germfree and conventional guinea pigs with two strains of trichomonas vaginalis. 196014426854
systemic treatment of trichomonas vaginalis infestation in women. a preliminary report. 196014436892
[on the parasiticidal effects of furazolidone and nifuroxime on trichomonas vaginalis]. 196214449156
[behavior of the uterine mucosa (in experimental animals) in intra-vaginal infection with trichomonas vaginalis]. 196214454701
[appearance of trichomonas vaginalis donne in girls in relation to hygienic conditions]. 196214458142
[cultivation of trichomonas vaginalis in various media]. 196214460177
terminal respiration in trichomonas vaginalis. 196214460577
[detection and biological properties of trichomonas vaginalis]. 196214460756
a pathognomonic colposcopic sign of trichomonas vaginalis vaginitis. 196114462060
influence of dichloroacetylation on the antimicrobial activity of chloramphenicol derivatives and of various amines.the dichloroacetyl group, one of the functional groups responsible for the antibacterial activity of chloramphenicol, has been inserted into various amines, and the dichloroacetamido-derivatives obtained have been studied against e. histolytica edm, candida albicans atcc 2091, saccharomyces cerevisiae atcc 7921, aspergillus niger nrrl 3, trichomonas vaginalis and mycobacterium tuberculosis h(37)rv and atcc 607. among the various chlorophenoxamide analogues, only n-(benzothiazol-2-ylmethyl)dichlo ...196114466299
[changes in the human vaginal epithelium during the course of therapy of trichomonas vaginalis infection]. 196214466785
[effect of mitotic poisons on the development of trichomonas vaginalis in culture]. 196214482298
flagyl in treatment of "trichomonas vaginalis" vaginitis. 196214485391
[laboratory demonstration of trichomonas vaginalis. comparison between direct microscopic study and culture]. 196214485885
disc testing of drugs against plated trichomonas vaginalis. 196214496691
[trichomonas vaginalis vaginitis]. 196114497160
vaginitis in a gynecologic practice in israel: causes and risk factors.vaginal symptoms are a leading reason for a patient to visit her gynecologist. little is known about the prevalence of the different causes of vaginitis and the risk factors for this entity in israel.200314509151
synthesis and antiparasitic activity of albendazole and mebendazole analogues.albendazole (abz) and mebendazole (mbz) analogues have been synthesized and in vitro tested against the protozoa giardia lamblia, trichomonas vaginalis and the helminths trichinella spiralis and caenorhabditis elegans. results indicate that compounds 4a, 4b (abz analogues), 12b and 20 (mbz analogues) are as active as antiprotozoal agents as metronidazole against g. lamblia. compound 9 was 58 times more active than abz against t. vaginalis. compounds 8 and 4a also shown high activity against this ...200314527558
effects of metronidazole and tinidazole on ntpdase1 and ecto-5'-nucleotidase from intact cells of trichomonas vaginalis.here we report the effects of metronidazole and tinidazole on ntpdase1 and ecto-5'-nucleotidase from intact cells of trichomonas vaginalis. adenosine triphosphate (atp) and adenosine diphosphate (adp) hydrolysis was 5- to 7-fold higher for the fresh clinical strain, when compared with the atcc (american type culture collection) strain. atp hydrolysis was activated in the presence of metronidazole in the atcc strain, whilst it was inhibited 33% by 50 microm tinidazole in a fresh clinical isolate. ...200314553936
glycogen phosphorylase sequences from the amitochondriate protists, trichomonas vaginalis, mastigamoeba balamuthi, entamoeba histolytica and giardia intestinalis.glycogen phosphorylase genes or messages from four amitochondriate eukaryotes, trichomonas vaginalis, mastigamoeba balamuthi, entamoeba histolytica (two genes) and giardia intestinalis, have been isolated and sequenced. the sequences of the amitochondriate protist enzymes appear to share a most recent common ancestor. the clade containing these sequences is closest to that of another protist, the slime mold (dictyostelium discoideum), and is more closely related to fungal and plant phosphorylase ...200314563176
sexually transmitted infections in male clients of female sex workers in benin: risk factors and reassessment of the leucocyte esterase dipstick for screening of urethral infections.(1) to assess risk factors for urethral infections with chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis among male clients of female sex workers (fsws) in benin; (2) to study the validity of led testing of male urine samples compared to a highly sensitive gold standard (pcr) for the diagnosis of urethral infections with the organisms cited above.200314573834
comparison of culture and different pcr assays for detection of trichomonas vaginalis in self collected vaginal swab specimens.dna amplification techniques have become widely used for the diagnosis of sexually transmitted infections. for the detection of trichomonas vaginalis, pcr techniques are not yet widely used despite the publication of several assays. the sensitivity and specificity of five independent primer sets were determined on self collected vaginal specimens obtained from female commercial sex workers.200314573835
newborn with suppurative nasal discharge. 200314579821
[bacterial flora in the genital tract the last trimester of pregnancy].very widespread in our clinical setting, early-onset sepsis is due to organisms that commonly colonize or infect the maternal genital tract; identifying such organisms would help improve prevention and treatment.200314593302
immunoblot analysis of trichomonas vaginalis antigens recognized by rabbit hyperimmune serum raised against exoantignes.the sds-page and immunoblot were used to identify trichomonas vaginalis antigenic epitopes present in purified somatic and exo-antigens. the presence of common immunogenic proteins corresponding to molecular weights of 76, 60 and 23 kda was revealed. distinctive immunogenic bands of 92, 72, 55 and 40 kda for the exo-antigens, and of 110, 80, 78 and 50 kda for the somatic antigens, appeared when the antigens were probed by the homologous immune rabbit serum.200414598168
trichomonas vaginalis among hiv-infected women: are immune status or protease inhibitor use associated with subsequent t. vaginalis positivity?trichomonas vaginalis (tv) is a common sexually transmitted disease (std) among hiv-infected populations. the relationship between tv and immune status and hiv viral load as affected by protease inhibitor (pi) use has not been well examined.200314603092
assessment of a rapid antigen detection system for trichomonas vaginalis infection. 200314607884
[therapeutic aspects of trichomoniasis].trichomoniasis is frequent, parasitic and sexually transmitted infection of genitourinary tract. it is treated by metronidazole (5-nitroimidazole), according to protocol recommended by center for disease control (cdc, formerly called: communicable disease center) [19]. the resistance of trichomonas vaginalis (tv) strains to metronidazole (mnd) was described in usa in 1960, and later on in many european countries [8, 9, 10, 11, 12, 13]. in these cases, due to persistent trichomonas infection, it ...200314608880
prevalence of sexually transmitted diseases among pregnant women in ilorin, nigeria.in a cross-sectional study, 230 pregnant women attending the antenatal clinic of the university of ilorin teaching hospital (uith), ilorin, nigeria from january 2000 to december 2000 were screened randomly to determine the prevalence of common stds among them, using conventional methods. one hundred and fourteen (49.4%) of the subjects harboured various agents including candida spp. (37.8%), trichomonas vaginalis (4.7%), gardnerella vaginalis (3.9%), syphilis (1.7%) and neisseria gonorrhea (1.3% ...200314617466
[is there a correlation between the vaginal colonization with group b streptococci and premature deliveries?].the study covered 110 pregnant females during the period from january 1, 2002 till april 30, 2003. nine females (8.18%) delivered premature newborns. samples were taken from the posterior vaginal fornix and canalis cervicalis in the 18th and 24th gestational week. the preparations stained after gram were examined under light microscope. microbial cultures on blood agar were examined, too. it was established that 18 or 16.36% of all the cases presented with cultures positive for group b streptoco ...200314619743
transcription initiation at its most basic level.the structure of the initiator binding protein from the primitive eukaryote trichomonas vaginalis reveals how a single protein can orchestrate promoter recognition, rna polymerase ii recruitment, and start site selection, and provides an evolutionary link with transcription initiation in higher eukaryotes.200314622590
structural basis of core promoter recognition in a primitive eukaryote.transcription start site selection in eukaryotes is mediated through combinations of the tata, initiator (inr), and downstream promoter elements (dpe). in trichomonas vaginalis, a parabasalian flagellate thought to represent an ancient eukaryote lineage, the inr appears to be solely responsible for start site selection and is recognized by the initiator binding protein 39 kda (ibp39). ibp39 contains an n-terminal inr binding domain (ibd) connected via a flexible linker to a c-terminal domain (c ...200314622596
trichomonas vaginalis: determination of acid phosphatase activity as a pharmacological screening procedure.a simple method to screen trichomonacides, based on the quantification of acid phosphatase (ap) activity, has been designed. using p-nitrophenyl phosphate as chromogenic substrate, we first determined the optimal conditions for enzyme reaction. after seeding, a linear correlation between number of trichomonads and optical densities at 405 nm was obtained at 24 hr but not at 48 hr. then, the inhibitory effect of metronidazole was assessed both by microscope counts and by ap determination. similar ...200314627165
[detection of trichomonas vaginalis with direct immunofluorescence assay].to detect trichomonas vaginalis (tv) by direct immunofluorescence assay (dfa) and discuss its clinical significance.200314628381
signs of infection in pap smears and risk of adverse pregnancy outcome.previous studies on whether signs of infection in pap smears imply a risk factor for preterm delivery are conflicting. in a large population-based study, we combined information from the swedish medical birth register and the swedish pap smear screening register to investigate whether signs of infection in pap smears increase the risks of adverse pregnancy outcomes. logistic regression analyses were used to estimate odds ratios (or) for antepartum death, preterm or small-for-gestational-age deli ...200314629315
antiparasitic activity of highly conjugated pyrimidine-2,4-dione derivatives.4-[2-(1,3-dimethyl-5-nitro-2,6-dioxo-1,2,3,6-tetrahydropyrimidin-4-yl)vinyl]benzaldehyde was synthesized in four steps from 6-methyl-1h,3h-pyrimidine-2,4-dione. this aldehyde was functionalized by various substituted anilines or substituted benzylamines. antiparasitic activities of the corresponding azomethines were assessed against plasmodium falciparum, trichomonas vaginalis and leishmania infantum compared to their toxicity versus human cells.200314630237
the amitochondriate eukaryote trichomonas vaginalis contains a divergent thioredoxin-linked peroxiredoxin antioxidant system.trichomonas is an amitochondriate parasitic protozoon specialized for an anaerobic lifestyle. nevertheless, it is exposed to oxygen and is able to cope with the resultant oxidative stress. in the absence of glutathione, cysteine has been thought to be the major antioxidant. we now report that the parasite contains thioredoxin reductase, which functions together with thioredoxin and thioredoxin peroxidase to detoxify potentially damaging oxidants. thioredoxin reductase and thioredoxin also reduce ...200414630923
treatment of trichomonas in pregnancy and adverse outcomes of pregnancy: a subanalysis of a randomized trial in rakai, uganda.the purpose of this study was to assess the association of presumptive trichomonas vaginalis treatment during pregnancy and birth outcomes.200314634576
vaginal cytokines in normal pregnancy.the purpose of this study was to determine whether the vaginal cytokine concentration varies during the course of uncomplicated pregnancy.200314634582
programmed cell death in parasitic protozoans that lack mitochondria. 200314642765
targeted gene replacement of a ferredoxin gene in trichomonas vaginalis does not lead to metronidazole resistance.ferredoxin, fd, is often deficient in metronidazole-resistant strains of trichomonas vaginalis and is thought to be necessary for drug activation. to directly test whether fd is essential for metronidazole susceptibility, gene replacement technology has been developed for t. vaginalis. the selectable marker gene neomycin phosphotransferase (neo) flanked by approximately 2.6 and approximately 2.0 kbp of the fd 5' and 3' flanking regions (pko-fd-neo) was introduced into cells on linear dna and sel ...200414651615
pseudocysts in trichomonads--new insights.tritrichomonas foetus and trichomonas vaginalis, parasitic protists of the urogenital tract, display a trophozoite and a pseudocyst stage. the ultrastructure of the trophozoite was compared with the pseudocyst form. the latter appears under unfavorable environmental conditions when the flagella are internalized, and a true cell wall is not formed. although some authors consider this form as a degenerate stage, the cell behaves as a resistant form. pseudocysts were found in natural culture condit ...200314658492
use of the roche lightcycler instrument in a real-time pcr for trichomonas vaginalis in urine samples from females and males.trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection (sti) that can result in vaginitis, urethritis, and preterm birth. traditional methods of diagnosis, including wet preparation, can be unreliable. in this study, we describe the adaptation of an existing pcr method for specific detection of t. vaginalis dna into a rapid real-time pcr assay based on fluorescence resonance energy transfer (fret) probe chemistry. the fret-based assay described demonstrated high ...200314662951
detection of tritrichomonas foetus by polymerase chain reaction in cultured isolates, cervicovaginal mucus, and formalin-fixed tissues from infected heifers and fetuses.a rapid, reliable polymerase chain reaction (pcr) assay, originally developed for definitive laboratory identification of the bovine venereal pathogen tritrichomonas foetus from cultures of male reproductive tract fluids, was used for testing the following: 1) cultured, geographically disparate trichomonad isolates, 2) formalin-fixed tissues from infected heifers and naturally infected fetuses, and 3) cervicovaginal mucus (cvm) from experimentally infected females. in 12 of 12 western hemisphere ...200314667024
human papillomavirus cervical infection in guarani indians from the rainforest of misiones, argentina.to evaluate the prevalence of human papillomavirus (hpv) cervical infection in women from the south american guarani indian tribe located in the rain forest of misiones, north-eastern argentina; a region with a high incidence of cervical carcinoma.200414690776
modeller: generation and refinement of homology-based protein structure models. 200314696385
effect of anapsos in a murine model of experimental trichomoniasis.immunomodulator effect of anapsos (polypodium leukotomas extract) in nmri (us naval medical research institute) outbred mice infected by the intraperitoneal route with 10(7) trichomonas vaginalis has been tested. gross histopathologic changes in abdominal organs and mortality rate, as a consequence of the pathogenicity of the protozoa and the immune response of the host, were evaluated. among the different treatment regimes assayed, anapsos at doses of 20 mg/kg/day administered for 10 days befor ...200314710626
sexually transmitted diseases.sexually transmitted diseases (stds) constitute a major health burden in the united states, causing pelvic inflammatory disease, ectopic pregnancy, infertility, chronic pelvic pain, genital lesions, genital neoplasms, adverse pregnancy outcomes, immune system dysfunction, liver disease, and even death. stds disproportionately affect adolescents and young adults. of the estimated 15 million stds that occur annually each year in the united states, 4 million are among adolescents and 6 million amon ...200314724772
summary: international conference on anaerobic protists. 200314736223
a 39-kda cysteine proteinase cp39 from trichomonas vaginalis, which is negatively affected by iron may be involved in trichomonal cytotoxicity. 200314736224
two trichomonas vaginalis loci encoding for distinct cysteine proteinases show a genomic linkage with putative inositol hexakisphosphate kinase (ip6k2) or an abc transporter gene. 200314736227
trichomoniasis: under control or undercontrolled?aside from human papillomavirus, trichomoniasis is the most common sexually transmitted infection in the united states today, yet it has long been regarded as a sexually transmitted infection of minor importance. medical opinion has traditionally held that it plays little role in health complications in women, and it is rarely seen in men. however, evidence has recently accumulated implicating trichomonas vaginalis as a contributor to a variety of adverse outcomes among both sexes. among both wo ...200414749674
a new method for extended trichomonad storage. 200414755043
synthesis, and antiprotozoal and antibacterial activities of s-substituted 4,6-dibromo- and 4,6-dichloro-2-mercaptobenzimidazoles.the synthesis and some germicidal activities in vitro of two congener series of s-substituted 4,6-dihalogeno-2-mercapto-1h-benzimidazoles are reported. there was no substantial difference between antibacterial activities of corresponding 4,6-dichloro- and 4,6-dibromo-derivatives. the present results confirm lower susceptibility to substituted benzimidazoles of gram-negative compared to gram-positive bacteria. minimum inhibitory concentrations (mics) of a majority of the novel derivatives ranged ...200414757505
epidemiology and etiology of sexually transmitted infection among hotel-based sex workers in dhaka, bangladesh.the prevalence of sexually transmitted infections (stis) and reproductive tract infections (rtis) among hotel-based sex workers (hbsws) in dhaka, bangladesh, was studied. a total of 400 hbsws were enrolled in the study during april to july 2002. endocervical swabs, high vaginal swabs, and blood samples from 400 hbsws were examined for neisseria gonorrhoeae (by culture), chlamydia trachomatis (by pcr), trichomonas vaginalis (by microscopy), antibody to treponema pallidum (by both rapid plasma rea ...200414766825
trichomonas vaginalis vaginitis; treatment with a new surface-active trichomonacide. 195014771180
a simplified casein hydrolysate-serum medium for the cultivation of trichomonas vaginalis. 195014771387
in vitro inhibition of growth of a pathogenic protozoan by a derivative of glutamic acid. 195014775664
survival of trichomonas vaginalis in vaginal discharge. 195014781172
[leukorrhea due to trichomonas vaginalis]. 195014784161
the diagnosis and treatment of trichomonas vaginalis. 195014788646
[vaginal smear and trichomoniasis]. 195014808772
beta-glucuronidase studies in women. iii. trichomonas vaginalis vaginitis and vaginal fluid enzyme activity. 195114810815
[culture of trichomonas vaginalis]. 195114845968
treatment of trichomonas vaginalis vaginitis. ii. factors affecting response to treatment. 195114850765
a new method of treatment of trichomonas vaginalis vaginitis. 195114855263
[laboratory diagnosis of trichomonas]. 195114856908
aureomycin in the therapy of trichomonas vaginalis vaginitis. 195114857086
trichomonas vaginalis infections treated with "penotrane". 195114858885
the incidence of trichomonas vaginalis in a sample of the general population, with some observations on its relation to the use of chemical contraceptives. 195114861681
[vaginitis due to trichomonas vaginalis]. 195114864911
treatment of trichomonas vaginalis vaginitis. 195114874113
the occurrence of trichomonas vaginalis in semen. 195114880979
[trichomonas vaginalis in prostitutes; possible role in urethritis in general]. 195114896304
[biology of trichomonas vaginalis culture]. 195114899463
the survival of trichomonas vaginalis at temperatures below +37 degrees c. 195114908048
vaginitis produced by trichomonas vaginalis; treatment with aureomycin and terramycin. 195114915220
aureomycin inserts in the treatment of trichomonas vaginalis vaginitis. 195214930201
[phase contrast microscopical detection of trichomonas vaginalis, including dead or degenerated protozoa]. 195214937271
in vivo susceptibility of trichomonas vaginalis to antibiotic therapy. 195214943911
[biological and clinical research on trichomonas vaginalis infection]. 195214944083
[culture of trichomonas vaginalis with johnson's and trussel's medium modified by pentinnen and raumano]. 195214944399
terramycin in the treatment of trichomonas vaginalis vaginitis. 195214945902
[morphological changes of trichomonas vaginalis]. 195214951443
the application of a haemagglutination technique to the study of trichomonas vaginalis infections. 195214955542
[on urethritis due to trichomonas vaginalis in man]. 195214957819
the in vitro effect of assafoetida on trichomonas vaginalis.assafoetida is an oleo-gum resin obtained from the roots and stems of many ferula species such as f. foetida regel, f. asafoetida linn, f. alliacea boiss. and f. narthex boiss, family umbelliferae (carrot family), plants growing indigenously in iran, afghanistan and north of india. assafoetida is commonly used as a flavoring agent in food and as a traditional medicine for many diseases in many parts of the world. in this work, the effect of assafoetida on growth of trichomonas vaginalis in vitro ...200314964671
cryopreservation of protozoan parasites.conventional methods for the propagation and preservation of parasites in vivo or in vitro have some limitations, including the need for labor, initial isolation and loss of strains, bacterial, and fungal contamination, and changes in the original biological and metabolic characteristics. all these disadvantages are considerably reduced by cryopreservation. in this study, we examined the effects of various freezing conditions on the survival of several protozoan parasites after cryopreservation. ...200414969677
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