Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
methyl salicylate level increase in flax after fusarium oxysporum infection is associated with phenylpropanoid pathway activation.flax (linum usitatissimum) is a crop plant valued for its oil and fiber. unfortunately, large losses in cultivation of this plant are caused by fungal infections, with fusarium oxysporum being one of its most dangerous pathogens. among the plant's defense strategies, changes in the expression of genes of the shikimate/phenylpropanoid/benzoate pathway and thus in phenolic contents occur. among the benzoates, salicylic acid, and its methylated form methyl salicylate play an important role in regul ...201628163709
small rnas regulate the biocontrol property of fluorescent pseudomonas strain psd.the production of biocontrol factors by pseudomonads is reported to be controlled at the post-transcriptional level by the gacs/gaca signal transduction pathway. this involves rna-binding translational repressor proteins, rsma and rsme, and the small regulatory rnas (srnas) rsmx, rsmy, and rsmz. while the former represses genes involved in secondary metabolite production, the latter relieves this repression at the end of exponential growth. we have studied the fluorescent pseudomonas strain psd, ...201728164793
a rare case of subcutaneous fusariosis in an immunocompetent patient.fusarium species can cause diseases in immunocompromised patients, whereas have rarely been reported as pathogens in immunocompetent individuals. fusarium oxysporum and f. solani species complex are the most frequent pathogenic species. primary subcutaneous hyalohyphomycosis due to these filamentous fungi is an extremely rare pathology, especially in immunocompetent patients. only 37 cases of deep fusarium infection in healthy patient have been reported in international literature. we report a c ...201728166614
enhancement of seed dehiscence by seed treatment with talaromyces flavus gg01 and gg04 in ginseng (panax ginseng).seed dehiscence of ginseng (panax ginseng c. a. mayer) is affected by moisture, temperature, storage conditions and microbes. several microbes were isolated from completely dehisced seed coat of ginseng cultivars, chunpoong and younpoong at gumsan, korea. we investigated the potential of five talaromyces flavus isolates from the dehiscence of ginseng seed in four traditional stratification facilities. the isolates showed antagonistic activities against fungal plant pathogens, such as cylindrocar ...201728167883
development of a hydrolysis probe-based real-time assay for the detection of tropical strains of fusarium oxysporum f. sp. cubense race 4.fusarium oxysporum f. sp. cubense (foc) is one of the most important threats to global banana production. strategies to control the pathogen are lacking, with plant resistance offering the only long-term solution, if sources of resistance are available. prevention of introduction of foc into disease-free areas thus remains a key strategy to continue sustainable banana production. in recent years, strains of foc affecting cavendish bananas have destroyed plantations in a number of countries in as ...201728178348
potential of burkholderia seminalis tc3.4.2r3 as biocontrol agent against fusarium oxysporum evaluated by mass spectrometry imaging.species of genus burkholderia display different interaction profiles in the environment, causing either several diseases in plants and animals or being beneficial to some plants, promoting their growth, and suppressing phytopathogens. burkholderia spp. also produce many types of biomolecules with antimicrobial activity, which may be commercially used to protect crops of economic interest, mainly against fungal diseases. herein we have applied matrix-assisted laser desorption/ionization mass spec ...201728194740
paenibacillus polymyxa nsy50 suppresses fusarium wilt in cucumbers by regulating the rhizospheric microbial community.paenibacillus polymyxa (p. polymyxa) nsy50, isolated from vinegar residue substrate, suppresses the growth of fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. the aim of the present study was to evaluate the effects of nsy50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to fusarium oxysporum. bacterial and fungal communities were investigated by illumina sequencing of t ...201728198807
draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant.here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes.201728209821
rapid and sensitive diagnoses of dry root rot pathogen of chickpea (rhizoctonia bataticola (taub.) butler) using loop-mediated isothermal amplification assay.dry root rot (drr) caused by the fungus rhizoctonia bataticola (taub.) butler, is an emerging disease in chickpea. the disease is often mistaken with other root rots like fusarium wilt, collar rot and black root rot in chickpea. therefore, its timely and specific detection is important. current detection protocols are either based on mycological methods or on protocols involving dna amplification by polymerase chain reaction (pcr). here we report the rapid and specific detection of r. bataticola ...201728218268
expression and distribution of extensins and agps in susceptible and resistant banana cultivars in response to wounding and fusarium oxysporum.banana fusarium wilt caused by fusarium oxysporum f. sp. cubense (foc) is soil-borne disease of banana (musa spp.) causing significant economic losses. extensins and arabinogalactan proteins (agps) are cell wall components important for pathogen defence. their significance for foc resistance in banana was not reported so far. in this study, two banana cultivars differing in foc sensitivity were used to monitor the changes in transcript levels, abundance and distribution of extensins and agps aft ...201728218299
enzyme inhibitory metabolites from endophytic penicillium citrinum isolated from boswellia sacra.fungal endophytes establish an important niche within the host plant through the secretion of chemical constituents. isolation of bioactive metabolites could be a vital source for inhibiting the function of enzymes such as α-glucosidase and urease. the present study aimed to elucidate the potential of endophytes associated with boswellia sacra through bioassay-guided isolation and identification of secondary metabolites with enzyme inhibitory ability. endophytic fungal strains viz. penicillium c ...201728220202
effectiveness of neutral electrolyzed water on incidence of fungal rot on tomato fruits ( solanum lycopersicum l.).we assessed the effect of neutral electrolyzed water (new) on the incidence of rot on tomato ( solanum lycopersicum l.) fruits inoculated with fusarium oxysporum , galactomyces geotrichum , and alternaria sp. at sites with lesions. the inoculated fruits were treated with new at 10, 30, and 60 mg liter(-1) active chlorine, with copper oxychloride fungicide, and with sterile distilled water (control) for 3, 5, and 10 min. in the experiment with f. oxysporum , 50 to 80% of the control fruits and 50 ...201628221850
multiple garlic (allium sativum l.) micrornas regulate the immunity against the basal rot fungus fusarium oxysporum f. sp. cepae.the basal plate rot fungus, fusarium oxysporum f. sp. cepae (foc), is the most devastating pathogen posing a serious threat to garlic (allium sativum l.) production worldwide. micrornas (mirnas) are key modulators of gene expression related to development and defense responses in eukaryotes. however, the mirna species associated with garlic immunity against foc are yet to be explored. in the present study, a small rna library developed from foc infected resistant garlic line was sequenced to ide ...201728224922
suppression of fusarium wilt of cucumber by ammonia gas fumigation via reduction of fusarium population in the field.cucumber plants subjected to consecutive monoculture for 9 years were found to suffer from severe fusarium wilt disease, caused by the soil-borne fungus fusarium oxysporum f. sp. cucumerinum j. h. owen. in the present study, greenhouse experiments were performed to evaluate the influence of ammonia gas fumigation on fusarium wilt suppression, fungal abundance and fungal community composition. results showed that ammonia gas fumigation remarkably reduced disease incidence from 80% to 27%, resulti ...201728230182
antimicrobial activity of medicinal plants correlates with the proportion of antagonistic endophytes.medicinal plants are known to harbor potential endophytic microbes, due to their bioactive compounds. in a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, hypericum perforatum and ziziphora capitata with contrasting antimicrobial activities from the chatkal biosphere reserve of uzbekistan, and their plant-specific traits involved in biocontrol and plant growth promotion were evaluated. plant extracts of h. perforatum exhibited a remarkable activity a ...201728232827
<i>fusarium</i> species isolated from <i>pennisetum clandestinum</i> collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201428235286
identification of biomarkers for resistance to fusarium oxysporum f. sp. cubense infection and in silico studies in musa paradisiaca cultivar puttabale through proteomic approach.panama wilt caused by fusarium oxysporum f. sp. cubense (foc) is one of the major disease constraints of banana production. previously, we reported the disease resistance musa paradisiaca cv. puttabale clones developed from ethylmethanesulfonate and foc culture filtrate against foc inoculation. here, the same resistant clones and susceptible clones were used for the study of protein accumulation against foc inoculation by two-dimensional gel electrophoresis (2-de), their expression pattern and a ...201628248219
response of atnpr1-expressing cotton plants to fusarium oxysporum f. sp. vasinfectum isolates.in our earlier investigation, we had demonstrated that transgenic cotton plants expressing atnpr1 showed significant tolerance to fusarium oxysporum f. sp. vasinfectum, isolate 11 (fov11) and several other pathogens. the current study was designed to further characterize the nature of the protection provided by atnpr1 expression and its limitations. green fluorescent protein-expressing fov11 was generated and used to study the progression of the disease within the plant. the results show that th ...201728250590
the plasma membrane-localized sucrose transporter ibsweet10 contributes to the resistance of sweet potato to fusarium oxysporum.sweet (sugars will eventually be exported transporter) proteins, a novel family of sugar transporters, mediate the diffusion of sugars across cell membranes and acts as key players in sucrose phloem loading. manipulation of sweet genes in plants leads to various effects on resistance to biotic and abiotic stresses due to disruption of sugar efflux and changes in sugar distribution. in this study, a member of the sweet gene family, ibsweet10, was cloned from the sweet potato line nd98. mrna expre ...201728261250
diversity, phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with monarda citriodora l.present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the monarda citriodora cerv. ex lag. (lamiaceae/labiatae). anticancer and antimicrobial potential of isolated endophytes have also been investigated.201728264654
disseminated phaeohyphomycosis in a dog.phaeohyphomycosis is a rare but emerging disease caused by dematiaceous fungi. here we describe the case of an immunosuppressed dog with disseminated phaeohyphomycosis secondary to bipolaris spicifera infection. regionally extensive infiltration of the paw pads, skin, myocardium, liver, renal interstitium and diaphragm was identified on histopathology. candida glabrata and fusarium oxysporum were also cultured from multiple sites post-mortem. the dog was treated with fluconazole, itraconazole, t ...201728275534
medium ph in submerged cultivation modulates differences in the intracellular protein profile of fusarium oxysporum.fusarium oxysporum is a filamentous fungus that damages a wide range of plants and thus causes severe crop losses. in fungal pathogens, the genes and proteins involved in virulence are known to be controlled by environmental ph. here, we report the influence of culture-medium ph (5, 6, 7, and 8) on the production of degradative enzymes involved in the pathogenesis of f. oxysporum urm 7401 and on the 2d-electrophoresis profile of intracellular proteins in this fungus. f. oxysporum urm 7401 was gr ...201728281888
nitrate increased cucumber tolerance to fusarium wilt by regulating fungal toxin production and distribution.cucumber fusarium wilt, induced by fusarium oxysporum f. sp. cucumerinum (foc), causes severe losses in cucumber yield and quality. nitrogen (n), as the most important mineral nutrient for plants, plays a critical role in plant-pathogen interactions. hydroponic assays were conducted to investigate the effects of different n forms (nh₄⁺ vs. no₃(‒)) and supply levels (low, 1 mm; high, 5 mm) on cucumber fusarium wilt. the no₃(‒)-fed cucumber plants were more tolerant to fusarium wilt compared with ...201728287458
draft genome sequence of rhizobium sp. strain tbd182, an antagonist of the plant-pathogenic fungus fusarium oxysporum, isolated from a novel hydroponics system using organic fertilizer.rhizobium sp. strain tbd182, isolated from a novel hydroponics system, is an antagonistic bacterium that inhibits the mycelial growth of fusarium oxysporum but does not eliminate the pathogen. we report the draft genome sequence of tbd182, which may contribute to elucidation of the molecular mechanisms of its fungistatic activity.201728302768
rhizosphere and root-infecting fungi and the design of ecological field experiments.as part of a wider study into the role of soil fungi in the ecology of the winter annual grass, vulpia ciliata ssp. ambigua (le gall) stace & auquier, we applied the fungicides benomyl and prochloraz to three natural populations of the grass growing in east anglia, united kingdom. the rhizosphere and rootinfecting fungi associated with the three populations were analysed each month between february and may 1992 when plants set seed. there were marked differences between the fungal floras associa ...199528306878
mineralization of cellulose in the presence of chitin and assemblages of microflora and fauna in soil.nitrogen content is an important factor controlling decomposition of resistant substrates. we examined the decomposition of purified cellulose in the presence of a structurally similar, resistant organic n compound, chitin. carbon-14-labelled cellulose was added to sterile ari-dried sandy loam soil in flasks, half of which were also amended with purified chitin. grassland soil organisms-a fungus (fusarium oxysporum) or a bacterium (flavobacterium sp.) with or without their respective nematode gr ...198328310541
plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato.fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ...201728321368
trans-cinnamic acid and xenorhabdus szentirmaii metabolites synergize the potency of some commercial fungicides.development of novel approaches for the control of fungal phytopathogens is desirable. in this study we hypothesized that the combination of commercial fungicides with certain enhancing agents could result in synergistic levels of control. prior research has indicated that trans-cinnamic-acid (tca), a metabolite of the bacteria photorhabdus luminescens and metabolites of xenorhabdus szentirmaii are particularly toxic to various phytpathogenic fungi when compared to metabolites of other xenorhabd ...201728322849
effector gene suites in some soil isolates of fusarium oxysporum are not sufficient predictors of vascular wilt in tomato.seventy-four f. oxysporum soil isolates were assayed for known effector genes present in an f. oxysporum f.sp. lycopersici race 3 tomato wilt strain (fol mn-25) obtained from the same fields in manatee county, florida. based on the presence or absence of these genes, four haplotypes were defined, two of which represented 96% of the surveyed isolates. these two most common effector haplotypes contained either all or none of the assayed race 3 effector genes. we hypothesized that soil isolates wit ...201728323535
comparison of dna microarray, loop-mediated isothermal amplification (lamp) and real-time pcr with dna sequencing for identification of fusarium spp. obtained from patients with hematologic malignancies.the performance of three molecular biology techniques, i.e., dna microarray, loop-mediated isothermal amplification (lamp), and real-time pcr were compared with dna sequencing for properly identification of 20 isolates of fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. dna microarray, lamp and real-time pcr identified 16 (80%) out of 20 samples as fusarium solani species complex (fssc) and four (20%) as fusarium spp. t ...201728324245
biosynthesis and characterization of a thermostable, alkali-tolerant chitinase from bacillus pumilus jubch08 displaying antagonism against phytopathogenic fusarium oxysporum.the present investigation highlights the process parameters influencing the submerged fermentation of chitinase by bacillus pumilus jubch08, purification and characterization of the enzyme and determination of antagonistic activity of the bacterium against fusarium oxysporum. medium supplemented with 0.5 % chitin and peptone, at initial ph 8.0, when incubated at 35 °c for 72 h favored highest chitinase production. the enzyme was purified 25.1-fold to homogeneity. the chitinase was found to be th ...201628330157
concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome.microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ...201728336093
members of gammaproteobacteria as indicator species of healthy banana plants on fusarium wilt-infested fields in central america.culminating in the 1950's, bananas, the world's most extensive perennial monoculture, suffered one of the most devastating disease epidemics in history. in latin america and the caribbean, fusarium wilt (fw) caused by the soil-borne fungus fusarium oxysporum f. sp. cubense (foc), forced the abandonment of the gros michel-based export banana industry. comparative microbiome analyses performed between healthy and diseased gros michel plants on fw-infested farms in nicaragua and costa rica revealed ...201728345666
antifungal activity, yield, and composition of ocimum gratissimum essential oil.ocimum gratissimum l. or clove basil, belongs to the lamiaceae family, has various desirable uses and applications. beyond its aromatic, seasoning, and medicinal applications, this plant also has antimicrobial activity. this study was aimed at assessing the antifungal activity, yield, and composition of the essential oil (eo) of o. gratissimum. the species was cultivated in garden beds with dystrophic red latosol soil type containing high organic-matter content. the eo was obtained by hydrodisti ...201728362991
biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem.the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ...201728364802
transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.fusarium oxysporum f. sp. melonis snyd. & hans race 1.2 (fom1.2) is the most virulent and yield-limiting pathogen of melon (cucumis melo l.) worldwide. current information suggest that the resistance to race 1.2 is controlled by multiple recessive genes and strongly affected by the environment. rna-seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes deployed during melon-fom1.2 interaction in the resistant doubled haploid line nad and in the ...201728367157
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
prospecting fungal parasites of the potato cyst nematode globodera pallida using a rapid screening technique.seven filamentous fungal species were isolated from individual eggs of globodera pallida cysts collected from infested fields in shelley idaho, usa and identified as chaetomium globosum, fusarium oxysporum, fusarium solani, fusarium tricinctum, microdochium bolleyi, purpureocillium lilacinum, and plectosphaerella cucumerina. their ability to reduce infection by g. pallida in planta were assessed in simple, reproducible micro-rhizosphere chambers (micro-rocs). all fungi reduced g. pallida infecti ...201728375550
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
investigation of genetic diversity of fusarium oxysporum f. sp. fragariae using pcr-rflp.fusarium wilts of strawberry, caused by fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. reliable chemical control of strawberry fusarium wilt disease is not yet available. moreover, other well-known f. oxysporum have different genetic information from f. oxysporum f. sp. fragariae. this analysis investigates the genetic diversity of strawberry fusairu ...201728381961
rapd assisted selection of black gram (vigna mungo l. hepper) towards the development of multiple disease resistant germplasm.black gram (vigna mungo l. hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. the yield potential of black gram is shallow due to lack of genetic variability and biotic stress susceptibility. core biotic stress factors include mung bean yellow mosaic virus (mymv), urdbean leaf crinkle virus (uclv), wilt (fusarium oxysporum) and powdery mildew (erysiphe polygoni dc). although many studies determine resistant varieties to a parti ...201728389895
involvement of salicylic acid, ethylene and jasmonate acid signaling pathways in susceptibility of tomato for fusarium oxysporum.phytohormones such as salicylic acid (sa), ethylene (et) and jasmonic acid (ja) play key roles in plant defense following pathogen attack. involvement of these hormones in susceptibility following fusarium oxysporum (fo) infection has mostly been studied in arabidopsis thaliana. fo, however, causes vascular wilt disease in a broad range of crops, including tomato (solanum lycopersicum). surprisingly little is known about the involvement of these phytohormones in susceptibility of tomato towards ...201728390170
methanolic extract of plumbago zeylanica - a remarkable antibacterial agent against many human and agricultural pathogens.the current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of plumbago zeylanica.201728392958
endophytic fungi associated with monarda citriodora, an aromatic and medicinal plant and their biocontrol potential.the food and agriculture organization has estimated that every year considerable losses of the food crops occur due to plant diseases. although fungicides are extensively used for management of plant diseases, they are expensive and hazardous to the environment and human health. alternatively, biological control is the safe way to overcome the effects of plant diseases and to sustain agriculture. since monarda citriodora cerv. ex lag. (lamiaceae/labiatae) is known for its antifungal properties, ...201728398103
immobilization of a cutinase from fusarium oxysporum and application in pineapple flavor synthesis.in the present study, the immobilization of a cutinase from fusarium oxysporum was carried out as cross-linked enzyme aggregates. under optimal immobilization conditions, acetonitrile was selected as precipitant, utilizing 9.4 mg protein/ml and 10 mm glutaraldehyde as cross-linker. the immobilized cutinase (imfocut5a) was tested in isooctane for the synthesis of short-chain butyrate esters, displaying enhanced thermostability compared to the free enzyme. pineapple flavor (butyl butyrate) synthes ...201728403608
nutritional composition and phytochemical, antioxidative, and antifungal activities of pergularia tomentosa l.crude extracts from a medicinal tunisian plant, pergularia tomentosa l., were the investigated natural material. butanolic extract of roots analyzed with ir spectra revealed the presence of hydroxyl, alcoholic, and carboxylic groups and sugars units. analysis of some secondary metabolites, total phenolic, flavonoids, flavonols, and procyanidins, was performed using different solvents following the increased gradient of polarity. fruits and leaves contained the highest amounts of all these compou ...201728409159
infection courts in watermelon plants leading to seed infestation by fusarium oxysporum f. sp. niveum.fusarium wilt incited by fusarium oxysporum f. sp. niveum (fon) is a seed-transmitted disease that causes significant yield loss in watermelon production. the pathogen may infect watermelon seeds latently, which can be an important inoculum source and contribute to severe disease outbreak. however, information regarding infection courts of fon leading to infestation of watermelon seeds is limited. to determine how seeds in watermelon fruit can be infested by fon during the watermelon growing sea ...201728414630
antimicrobial textiles: biogenic silver nanoparticles against candida and xanthomonas.this paper introduces cotton fibers impregnated with biogenic silver nanoparticles (agnps), synthesized from a fusarium oxysporum fungal filtrate (ff) solution, and open up the possibility for their use in medical environment and agriculture clothing as means to avoid microbial spreading. after thorough agnps characterization, regarding their physical, chemical and biochemical properties, minimum inhibitory concentrations (mic) against some human and orange tree pathogens were determined. we rep ...201728415502
essential oils as biocides for the control of fungal infections and devastating pest (tuta absoluta) of tomato (lycopersicon esculentum mill.).thymus capitatus and tetraclinis articulata essential oils as well their major components (carvacrol and α-pinene) were evaluated for their antifungal and insecticidal activities. both oils showed good in vitro antifungal activity against fusarium oxysporum, aspergillus niger, penicillium sp., alternaria alternata, and botrytis cinerea, the fungi causing tomato rot. in vivo results indicate the efficacies of both essential oils and carvacrol of reduce postharvest fungal pathogens, such as b. cin ...201728422413
the antibiotic polymyxin b exhibits novel antifungal activity against fusarium species.the genus fusarium comprises many species, including fusarium oxysporum, fusarium solani, fusarium graminearum and fusarium verticillioides, and causes severe infections in plants and humans. in clinical settings, fusarium is the third most frequent mould to cause invasive fungal infections after aspergillus and the mucorales. f. solani and f. oxysporum are the most prevalent fusarium spp. causing clinical disease. however, few effective antifungal drugs are available to treat human and plant fu ...201728433743
mediator18 and mediator20 confer susceptibility to fusarium oxysporum in arabidopsis thaliana.the conserved protein complex known as mediator conveys transcriptional signals by acting as an intermediary between transcription factors and rna polymerase ii. as a result, mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. in this report we identify the head domain subunits mediator18 and mediator20 as important susceptibility factors for fusarium oxysporum infection in arabidopsis thaliana. mutants of med18 and med20 display down- ...201728441405
design, synthesis of novel chitosan derivatives bearing quaternary phosphonium salts and evaluation of antifungal activity.two novel chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized, including tricyclohexylphosphonium acetyl chitosan chloride (tcpacsc) and triphenylphosphonium acetyl chitosan chloride (tppacsc), and characterized by ftir, (1)h nmr, and (13)c nmr spectra. the degree of substitution was also calculated by elemental analysis results. their antifungal activities against colletotrichum lagenarium, watermelon fusarium, and fusarium oxysporum were investigated i ...201728445700
genome-wide analysis of autophagy-related genes in banana highlights maatg8s in cell death and autophagy in immune response to fusarium wilt.maatg8s play important roles in hypersensitive-like cell death and immune response, and autophagy is essential for disease resistance against foc in banana. autophagy is responsible for the degradation of damaged cytoplasmic constituents in the lysosomes or vacuoles. although the effects of autophagy have been extensively revealed in model plants, the possible roles of autophagy-related gene in banana remain unknown. in this study, 32 maatgs were identified in the draft genome, and the profiles ...201728451821
comparative transcriptomic analysis of race 1 and race 4 of fusarium oxysporum f. sp. cubense induced with different carbon sources.the fungal pathogen fusarium oxysporum f. sp. cubense causes fusarium wilt, one of the most destructive diseases in banana and plantain cultivars. pathogenic race 1 attacks the "gros michel" banana cultivar, and race 4 is pathogenic to the cavendish banana cultivar and those cultivars that are susceptible to foc1. to understand the divergence in gene expression modules between the two races during degradation of the host cell wall, we performed rna sequencing to compare the genome-wide transcrip ...201728468818
plipastatin and surfactin coproduction by bacillus subtilis pb2-l and their effects on microorganisms.to convert the lipopeptide non-producer strain bacillus subtilis pb2 into a plipastatin and surfactin coproducer, a gene expression cassette composed of a constitutive promoter (p43), functional gene sfp, and pleiotropic regulatory gene degq was integrated into the chromosomal amye locus of strain b. subtilis pb2 by homologous recombination, which generated a plipastatin and surfactin co-producer. thirteen plipastatins and fifteen surfactins were identified in lipopeptide extracts using analytic ...201728477175
studies on chemical composition and antimicrobial activities of bioactive molecules from date palm (phoenixdactylifera l.) pollens and seeds.natural resources have been the crucial origin of chemical elements. they have been used in many traditions as alternative medicines. the chemical profiling of some plant extracts and essential oils related to different plants were followed to unveil their most active components. in this paper, phoenix dactilyfera l was selected as a host plant to investigate the composition of different organs with different cultivars.201728480436
in vitro antifungal, probiotic, and antioxidant functional properties of a novel lactobacillus paraplantarum isolated from fermented dates in saudi arabia.fermented foods produced using dates are used in gulf countries as beneficial and healthful foods. the beneficial microbial flora in fermented dates contributes to maintaining the nutritional properties of dates by preventing the growth of spoilage fungi. here, we examined the antifungal, probiotic, and antioxidant properties of the novel lactobacillus strain d-3 isolated from fermented dates.201728480570
the endochitinase vdech from verticillium dahliae inhibits spore germination and activates plant defense responses.chitinases function in the digestion of chitin molecules, which are present principally in insects and fungi. in plants, chitinase genes play important roles in defense, and their expression can be triggered in response to both biotic and abiotic stresses. in this study, we cloned and characterized an endochitinase (vdech) from verticillium dahliae, strain vd080. the vdech coding region consists of 1845bp with two exons and one 54bp intron, encoding a 615 amino acid protein with the predicted mo ...201728483050
fusarium oxysporum and the fusarium wilt syndrome.the fusarium oxysporum species complex (fosc) comprises a multitude of strains that cause vascular wilt diseases of economically important crops throughout the world. although sexual reproduction is unknown in the fosc, horizontal gene transfer may contribute to the observed diversity in pathogenic strains. development of disease in a susceptible crop requires f. oxysporum to advance through a series of transitions, beginning with spore germination and culminating with establishment of a systemi ...201728489498
fungal endophytes isolated from protium heptaphyllum and trattinnickia rhoifolia as antagonists of fusarium oxysporum.control of fungal pathogens is mainly addressed by the use of chemically synthesized fungicides which result in environmental pollution, developing resistance after prolonged use. in this context, endophytes have been recognized as potential biocontrollers, and also as a promising source of antifungal metabolites. therefore, as part of our research on phytopathogen controllers, 355 fungal endophytes were isolated from protium heptaphyllum and trattinnickia rhoifolia (burseraceae), both ethnobota ...201728495036
isolated bacillus subtilis strain 330-2 and its antagonistic genes identified by the removing pcr.plant growth-promoting bacteria (pgpb) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. in this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. based on 16s rdna and biolog test analysis, the 330-2 strain was identified as bacillus subtilis. the strain produced indole-3-acetic acid, siderophores, lytic e ...201728496135
effectiveness of a full-scale horizontal slow sand filter for controlling phytopathogens in recirculating hydroponics: from microbial isolation to full microbiome assessment.the microbial disinfestation efficiency of an innovative horizontal-flow slow sand filter (hssf) for treating nutrient solution spent from an experimental closed-loop nursery was evaluated by means of a combination of culture-dependent and independent molecular techniques. a dense inoculum of the fungal plant pathogen fusarium oxysporum f.sp. lycopersici was applied in the fertigation system (10(6) cells per ml). indigenous and introduced populations of eubacteria and fungi were assessed in the ...201728499226
purification and site-directed mutagenesis of linoleate 9s-dioxygenase-allene oxide synthase of fusarium oxysporum confirms the oxygenation mechanism.plants and fungi form jasmonic acid from α-linolenic acid. the first two steps of biosynthesis in plants occur by sequential transformation by 13s-lipoxygenase and allene oxide synthase (aos). the biosynthesis in fungi may follow this classical scheme, but the only fungal aos discovered so far are cytochromes p450 (cyp) fused to 8- and 9-dioxygenases (dox). in the present report, we purified recombinant 9s-dox-aos of fusarium oxysporum from cell lysate by cobalt affinity chromatography to near h ...201728502466
non-edible oil cakes as a novel substrate for dpa production and augmenting biocontrol activity of paecilomyces variotii.the present study investigated the use of waste non-edible oil cakes (jatropha, karanja, neem, and mahua) as a substrate for the growth of paecilomyces variotii and dipicolinic acid (dpa) production. previous researches proved the efficacy of dpa in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. dpa production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (dextrose, glucose, and lactose) as carbon source. ...201728512455
wble2 transcription factor in streptomyces griseus s4-7 plays an important role in plant protection.streptomyces griseus s4-7 was originally isolated from the strawberry rhizosphere as a microbial agent responsible for fusarium wilt suppressive soils. s. griseus s4-7 shows specific and pronounced antifungal activity against fusarium oxysporum f. sp. fragariae. in the streptomyces genus, the whi transcription factors are regulators of sporulation, cell differentiation, septation, and secondary metabolites production. wble2 function as a regulator has emerged as a new group in whi transcription ...201728523731
lipopeptides produced by bacillus subtilis as new biocontrol products against fusariosis in ornamental plants.in this study, we have investigated the effects of three lipopeptides (fengycin, surfactin and mycosubtilin) produced by different strains of bacillus subtilis against the phytopathogenic fungi fusarium oxysporum f. sp. iridacearum, which affects the ornamental bulb plant populations of iris sp. the antifungal effects were tested using minimum inhibitory concentration assay, determination of mycelium growth and spore germination inhibition rates. also, in vivo tests on infected rhizomes and scan ...201728528498
detoxification of fusaric acid by the soil microbe mucor rouxii.fusarium oxysporum f. sp. vasinfectum race 4 (vcg0114), which causes root rot and wilt of cotton (gossypium hirsutum and g. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the san joaquin valley of california. this pathotype produces copious quantities of the plant toxin fusaric acid (5-butyl-2-pyridinecarboxylic acid) compared to other isolates of f. oxysporum f. sp. vasinfectum (fov) that are indigenous to the united states. fusaric a ...201728538103
characterization of silver nanoparticles produced by biosynthesis mediated by fusarium oxysporum under different processing conditions.the goal of this study was the biosynthesis of silver nanoparticles (snps) mediated by the fungus fusarium oxysporum, as well as the characterization of these nanoparticles including evaluation of the particles size and stability under different processing conditions. the results showed that the biosynthesis produced silver nanoparticles having a mean size of 34 nm and zeta potential values below -30 mv at the conditions used, characterizing the nanoparticles as being stable in suspension. ultra ...201728540439
transcriptomic dissection reveals wide spread differential expression in chickpea during early time points of fusarium oxysporum f. sp. ciceri race 1 attack.plants' reaction to underground microorganisms is complex as sessile nature of plants compels them to prioritize their responses to diverse microorganisms both pathogenic and symbiotic. roots of important crops are directly exposed to diverse microorganisms, but investigations involving root pathogens are significantly less. thus, more studies involving root pathogens and their target crops are necessitated to enrich the understanding of underground interactions. present study reported the molec ...201728542579
analysis of protein profile of tomato root infected with fusarium oxysporum f. sp lycopersici.fusarium wilt caused by fusarium oxysporum f. sp lycopersici (fol) is one of the main diseases affecting tomatoes. the bhrs 2,3 genotype of tomato is, however, resistant to this disease. a proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. protein was extracted and separated by two-dimensional electrophoresis followed by staining with coomassie brilliant blue. the proteins were ide ...201728549197
clean up fly ash from coal burning plants by new isolated fungi fusarium oxysporum and penicillium glabrum.in turkey approximately 45 million tons of coals are burned in a year and 19.3 million tons of fly ash have emerged. the bioremediation of heavy metals or different elements from fly ash makes them bio-available. however, in previous studies, requiring of long operational time and failing to show tolerance to high pulp densities of fly ash of selected fungal species makes them impractical. in this work, bioremediation of fly ash by new isolated fungi fusarium oxysporum and penicillium glabrum we ...201728554126
a novel α-galactosidase from fusarium oxysporum and its application in determining the structure of the gum arabic side chain.we previously reported that fusarium oxysporum 12s produces two bifunctional proteins, foap1 and foap2, with α-d-galactopyranosidase (gpase) and β-l-arabinopyranosidase (apase) activities. the aim of this paper was to purify a third gpase, fogp1, from culture supernatant of f. oxysporum 12s, to characterize it, and to determine its mode of action towards gum arabic. a cdna encoding fogp1 was cloned and the protein was overexpressed in escherichia coli. module sequence analysis revealed the prese ...201728554382
hairy root transgene expression analysis of a secretory peroxidase (pvpox1) from common bean infected by fusarium wilt.plant peroxidases (poxs) are one of the most important redox enzymes in the defense responses. however, the large number of different plant pox genes makes it necessary to carefully confirm the function of each paralogous pox gene in specific tissues and disease interactions. fusarium wilt is a devastating disease of common bean caused by fusarium oxysporum f. sp. phaseoli. in this study, we evaluated a peroxidase gene, pvpox1, from a resistant common bean genotype, caas260205 and provided direc ...201728554466
production, characterization, and antifungal activity of a biosurfactant produced by rhodotorula babjevae ys3.sophorolipids are one of the most promising glycolipid biosurfactants and have been successfully employed in bioremediation and various other industrial sectors. they have also been described to exhibit antimicrobial activity against different bacterial species. nevertheless, previous literature pertaining to the antifungal activity of sophorolipids are limited indicating the need for further research to explore novel strains with wide antimicrobial activity. a novel yeast strain, rhodotorula ba ...201728558761
small rna sequencing for secondary metabolite analysis in persicaria minor.persicaria minor (kesum) is an important medicinal plant and commonly found in southeast countries; malaysia, thailand, indonesia, and vietnam. this plant is enriched with a variety of secondary metabolites (sms), and among these sms, terpenoids are in high abundance. terpenoids are comprised of many valuable biomolecules which have well-established role in agriculture and pharmaceutical industry. in p. minor, for the first time, we have generated small rnas data sets, which can be used as tool ...201728560169
illumina-based sequencing analysis directed selection for actinobacterial probiotic candidates for banana plants.as potential probiotic candidates, plant vertically transmitted actinobacteria are beneficial to growth and health of host plants. new methods to isolate the actinobacterial taxa with low growth rates should be developed. based on the actinobacterial population information, the probiotic actinobacterial taxa could be directly isolated from healthy banana shoot tips. however, actinobacterial dnas with high gc contents could bias estimates of actinobacteria by pcr. in the study, two amplicon seque ...201728560514
tobacco rattle virus-based silencing of enoyl-coa reductase gene and its role in resistance against cotton wilt disease.a tobacco rattle virus (trv)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton (gossypium hirsutum). this approach was used to investigate the function of the enoyl-coa reductase (ghecr) gene in pathogen defense. amino acid sequence alignment of arabidopsis ecr with homologous sequence from g. hirsutum, g. arboreum, g. herbaceum and g. barbadense showed that ecrs are highly conserved among these species. trv-based silencing of gh ...201728567686
an allene oxide and 12-oxophytodienoic acid are key intermediates in jasmonic acid biosynthesis by fusarium oxysporum.fungi can produce jasmonic acid (ja) and its isoleucine conjugate in large quantities, but little is known about the biosynthesis. plants form ja from 18:3n-3 by 13s-lipoxygenase (lox), allene oxide synthase, and allene oxide cyclase. shaking cultures of fusarium oxysporum f. sp. tulipae released over 200 mg of jasmonates per liter. nitrogen powder of the mycelia expressed 10r-dioxygenase-epoxy alcohol synthase activities, which was confirmed by comparison with the recombinant enzyme. the 13s-lo ...201728572515
corrigendum: transcriptome analysis of the melon-fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.[this corrects the article on p. 362 in vol. 8, pmid: 28367157.].201728572815
application of surface modified nano ferrite nickel in catalytic reaction (epoxidation of alkenes) and investigation on its antibacterial and antifungal activities.newly, magnetic nanoparticles have extensively been used as alternative catalyst supports, in the view of their high surface area which results in high catalyst loading capacity, high dispersion, low toxicity, environmental preservation, distinguished stability, and suitable catalyst reusing. in the present study, the magnetite nanoparticles, nife2o4@ag and nife2o4@mo, were synthesized and characterized. the antimicrobial activities and catalytic properties of synthesized nanoparticles were test ...201728575933
biodiversity in targeted metabolomics analysis of filamentous fungal pathogens by (1)h nmr-based studies.the taxonomical classification among fungi kingdom in the last decades was evolved. in this work the targeted metabolomics study based on (1)h nmr spectroscopy combined with chemometrics tools was reported to be useful for differentiation of three model of fungal strains, which represent various genus of ascomycota (aspergillus pallidofulvus, fusarium oxysporum, geotrichum candidum) were selected in order to perform metabolomics studies. each tested species, revealed specific metabolic profile o ...201728585165
specific pcr detection of fusarium oxysporum f. sp. raphani: a causal agent of fusarium wilt on radish plants.fusarium oxysporum, a causal agent of fusarium wilt, is one of the most important fungal pathogens worldwide, and detection of f. oxysporum dna at the forma specialis level is crucial for disease diagnosis and control. in this study, two novel f. oxysporum f. sp. raphani (for)-specific primer sets were designed, for1-f/for1-r and for2-f/for2-r, to target foqg_17868 and foqg_17869 orfs, respectively, which were selected based on the genome comparison of other formae speciales of f. oxysporum incl ...201728585248
evaluation of the in vitro antimicrobial activity of selected saudi scorpion venoms tested against multidrug-resistant micro-organisms.scorpion venoms are a rich source of bioactive peptides with promising clinical value that may lead to the discovery and development of new drugs. the present study was designed to evaluate the in vitro antimicrobial activities of the venoms extracted from three medically important saudi scorpions (androctonus crassicauda, androctonus bicolor and leiurus quinquestriatus).201728587870
polyketide-terpene hybrid metabolites from an endolichenic fungus pestalotiopsis sp.five new polyketide-terpene hybrid metabolites (1-5) with highly functionalized groups, together with six known derivatives (6-11), were isolated from the endolichenic fungus pestalotiopsis sp. their structures were elucidated by extensive nmr experiments including (1)h, (13)c, hmqc, cosy, and hmbc. the relative configurations of the new compounds were determined by analysis of coupling constants and roesy correlations. the absolute configurations especially the secondary alcohol at c-15 in 1 an ...201728593175
purification, characterization and retting of crotolaria juncea fibres by an alkaline pectin lyase from fusarium oxysporum mtcc 1755.using solid-state fermentation, production of an industrially important pectin lyase from a fungal strain fusarium oxysporum mtcc 1755 was attempted, which was further subjected to purification and characterization. the enzyme was purified by three steps, namely ammonium sulfate fractionation, cation-exchange chromatography on cm cellulose followed by gel filtration chromatography using sephadex g-100 column. a 16-fold purification with 31.2% yield and 3.2 u/mg specific activity was achieved. th ...201728593518
in vitro and in vivo antifungal efficacy of plant based lawsone against fusarium oxysporum species complex.fusarium oxysporum is an ascomycete facultative fungus which generally affects to plants. however, it is recently known as a serious emerging opportunistic pathogen of human and other animals. f. oxysporum shows broad resistance to commonly used antifungal agents and therefore development of alternative therapeutic agents is required. in this study, we investigated the antifungal efficacy of plant based natural lawsone against pathogenic f. oxysporum. antifungal susceptibility test determined th ...201728602398
fusarium infection causes genotoxic disorders and antioxidant-based damages in orobanche spp.this study aims to evaluate the toxic effects of fusarium oxysporum on root parasitic weed, orobanche spp. comparative genetic and gene expression studies were conducted on uninfected and fungus-infected orobanches. in genetic studies, isolated total dna was amplified by rapd pcr. fragment properties were analysed by gts test. according to the results, the fragment properties of control and fusarium infected (experimental) groups varied widely; and it has been observed that fusarium has genotoxi ...201728602401
the arabidopsis leucine-rich repeat receptor kinase mik2/lrr-kiss connects cell wall integrity sensing, root growth and response to abiotic and biotic stresses.plants actively perceive and respond to perturbations in their cell walls which arise during growth, biotic and abiotic stresses. however, few components involved in plant cell wall integrity sensing have been described to date. using a reverse-genetic approach, we identified the arabidopsis thaliana leucine-rich repeat receptor kinase mik2 as an important regulator of cell wall damage responses triggered upon cellulose biosynthesis inhibition. indeed, loss-of-function mik2 alleles are strongly ...201728604776
different mechanisms of trichoderma virens-mediated resistance in tomato against fusarium wilt involve the jasmonic and salicylic acid pathways.in the present study, we investigated the role of trichoderma virens (triv_jsb100) spores or cell-free culture filtrate in the regulation of growth and activation of the defence responses of tomato (solanum lycopersicum) plants against fusarium oxysporum f. sp. lycopersici by the development of a biocontrol-plant-pathogen interaction system. two-week-old tomato seedlings primed with triv_jsb100 spores cultured on barley grains (bgs) or with cell-free culture filtrate (cf) were inoculated with fu ...201728605157
chemical composition and antifungal activity of hedera helix leaf ethanolic extract.the 50% ethanol extract obtained from hedera helix leaves was investigated regarding the presence and quantity of polyphenols, sterols and in vitro antifungal activity against phytopathogenic fungi. the chemical analysis revealed the presence of rutin, quercetin and kaempferol in the non-hydrolysed sample and quercetin and kaempferol in the hydrolysed sample and stigmasterol in the ivy leaf extract (nonhydrolysed sample). the antifungal activity against phytopathogenic fungi (aspergillus niger, ...201728605982
identification of a potential isr determinant from pseudomonas aeruginosa pm12 against fusarium wilt in tomato.biocontrol of plant diseases through induction of systemic resistance is an environmental friendly substitute to chemicals in crop protection measures. different biotic and abiotic elicitors can trigger the plant for induced resistance. present study was designed to explore the potential of pseudomonas aeruginosa pm12 in inducing systemic resistance in tomato against fusarium wilt. initially the bioactive compound, responsible for isr, was separated and identified from extracellular filtrate of ...201728620396
a novel photosensitization treatment for the inactivation of fungal spores and cells mediated by curcumin.the global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. the effect of photosensitization mediated by curcumin on aspergillus niger, aspergillus flavus, penicillium gris ...201728623822
isolation of a potential biocontrol agent paenibacillus polymyxa nsy50 from vinegar waste compost and its induction of host defense responses against fusarium wilt of cucumber.fusarium wilt caused by fusarium oxysporum f. sp. cucumerinum (foc) is one of the major destructive soil-borne diseases infecting cucumber. in this study, we screened 60 target strains isolated from vinegar waste compost, from which 10 antagonistic strains were identified to have the disease suppression capacity of bio-control agents. the 16s rdna gene demonstrated that the biocontrol agents were paenibacillus polymyxa (p. polymyxa), bacillus amyloliquefaciens (b. amyloliquefaciens) and bacillus ...201728647117
a meta-analysis of quantitative trait loci for abiotic and biotic stress resistance in tetraploid cotton.the number and location of mapped quantitative trait loci (qtl) depend on genetic populations and testing environments. the identification of consistent qtl across genetic backgrounds and environments is a pre-requisite to marker-assisted selection. this study analyzed a total of 661 abiotic and biotic stress resistance qtl based on our previous work and other publications using the meta-analysis software biomercator. it identified chromosomal regions containing qtl clusters for different resist ...201728647758
an evaluation method for suppression of pathogenic fusarium oxysporum by soil microorganisms using the dilution plate technique.volume 31, no. 3, page 307-313, 2016page 310, legend for fig. 4 incorrectfig. 4. growth degree of fusarium oxysporum f. sp. spinaciae for organic fertilizers at each dilution based on an estimation of the ellipse area (a) and extension length (b) of the colony. cont, compound inorganic fertilizer; sbm, steamed bone meal; cdc, cow dung compost; mi, microbial inoculant. values show mean of medians of degrees with se (n=3). the ellipse area and extension length for control plates were 4,475 mm(2) a ...201728652549
novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi.tomato wilt pathogen fusarium oxysporum f. sp. lycopersici (fol) is grouped into three races based on their pathogenicity to different host cultivars. rapid detection and discrimination of fol races in field soils is important to prevent tomato wilt disease. although five types of point mutations in secreted in xylem 3 (six3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. the aim of this study is t ...201728652587
genetic diversity and antifungal susceptibility of fusarium isolates in onychomycosis.fusarium species have emerged as an important human pathogen in skin disease, onychomycosis, keratitis and invasive disease. onychomycosis caused by fusarium spp. the infection has been increasingly described in the immunocompetent and immunosuppressed hosts. considering onychomycosis is a difficult to treat infection, and little is known about the genetic variability and susceptibility pattern of fusarium spp., further studies are necessary to understand the pathogenesis and better to define th ...201728657120
antifungal activity and expression patterns of extracellular chitinase and β-1,3-glucanase in wickerhamomyces anomalus eg2 treated with chitin and glucan.in this study, the expression patterns of extracellular chitinase and β-1,3-glucanase from cultured wickerhamomyces anomalus eg2 treated with chitin, glucan, and chemical chitinase inhibitors (kinetin, caffeine, and acetazolamide) were investigated using sds-page. relationship between enzyme expression and antifungal activity from yeast plays a very important role for biocontrol of phytopathoges. to determine antifungal activity against phytopathogens, w. anomalus eg2 was shown to strongly inhib ...201728668604
isolation, identification, optimization, and metabolite profiling of streptomyces sparsus vsm-30.deep sea sediment samples of bay of bengal (visakhapatnam) have been analyzed for actinomycetes as an elite source to screen for the production of bioactive metabolites. the actinomycetes strain vsm-30 has an exciting bioactivity profile and was isolated during our systemic screening of marine actinomycetes. it was identified as streptomyces sparsus based on morphological, physiological, biochemical, and molecular approaches. response surface methodology regression analysis was carried out to fi ...201728669076
host-induced silencing of pathogenicity genes enhances resistance to fusarium oxysporum wilt in tomato.this study presents a novel approach of controlling vascular wilt in tomato by rnai expression directed to pathogenicity genes of fusarium oxysporum f. sp. lycopersici. vascular wilt of tomato caused by fusarium oxysporum f. sp. lycopersici leads to qualitative and quantitative loss of the crop. limitation in the existing control measures necessitates the development of alternative strategies to increase resistance in the plants against pathogens. recent findings paved way to rnai, as a promisin ...201728674943
Displaying items 2801 - 2900 of 2935