Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| assessment of phytochemicals, antimicrobial and cytotoxic activities of extract and fractions from fagonia olivieri (zygophyllaceae). | in pakistan fagonia olivieri (zygophyllaceae) is commonly used in the indigenous system of medicine for treatment of conditions like diabetes, cancer, fever, asthma, toothache, stomach troubles and kidney disorders. this study evaluated the crude methanol extract of f. olivieri (fom) and its derived fractions for their antimicrobial and cytotoxic activities as well as the classes of phytochemical. | 2013 | 23842440 |
| inactivation of microorganisms by low-frequency high-power ultrasound: 2. a simple model for the inactivation mechanism. | a simple theoretical model based on shear forces generated by the collapse of the ultrasound cavities near the surface of a microorganism is proposed. this model requires two parameters which take into account the number of acoustic cavitation bubbles, and the resistance of the cell wall of the microorganism to the shear forces generated by bubble collapse. to validate the model, high-power low frequency (20 khz) ultrasound was used to inactivate two microorganisms with very different sizes, viz ... | 2014 | 23845410 |
| purpurides b and c, two new sesquiterpene esters from the aciduric fungus penicillium purpurogenum js03-21. | purpurides b and c (1 and 2, resp.), two new sesquiterpene alcohol esters generated from a drimane-type sesquiterpenoid lactone and an amino acid, together with two known analogs, berkedrimane b (3) and purpuride (4), were isolated from the aciduric fungus penicillium purpurogenum js03-21. their structures were elucidated by spectroscopic analysis, x-ray single-crystal diffraction, and application of marfey's method. compounds 1-4 showed modest antifungal activities against candida albicans with ... | 2013 | 23847064 |
| the new species enterobacter oryziphilus sp. nov. and enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice. | six independent gram-negative, facultatively anaerobic, non-spore-forming, nitrogen-fixing rod-shaped isolates were obtained from the root endosphere of rice grown at the international rice research institute (irri) and investigated in a polyphasic taxonomic study. | 2013 | 23865888 |
| sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains. | most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. we developed a sulfatase-activated probe, 7-hydroxy-9h-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid det ... | 2013 | 23878250 |
| extraction, gas chromatography-mass spectrometry analysis and screening of fruits of terminalia chebula retz. for its antimicrobial potential. | terminalia chebula is called the "king of medicines" in tibet and is always listed first in the ayurvedic meteria medica because of its extraordinary powers of healing. | 2013 | 23901211 |
| studies on bioflocculant production by a mixed culture of methylobacterium sp. obi and actinobacterium sp. mayor. | bioflocculants effect the aggregation of suspended solutes in solutions thus, a viable alternative to inorganic poly-ionic and synthetic organic flocculants which are associated with deleterious health problems. consequently, a consortium of two bacteria species were evaluated for optimized bioflocculant yield following the inadequacies of axenic cultures. | 2013 | 23915393 |
| synthesis, sar and antibacterial activity of hybrid chloro, dichloro-phenylthiazolyl-s-triazines. | a series of hybrid novel chloro (1a-9a) and dichloro (10b-18b) phenylthiazolyl-s-triazine were synthesized and subsequently subjected to their antibacterial activity against three gram positive viz. lactobacillus casei (ncim-2651); bacillus cereus (ncim-2458); staphylococcus aureus (ncim-2120) and three gram negative viz salmonella typhimurium (ncim-2501); escherichia coli (ncim-2065); klebsiella aerogenes (ncim-2098). the sar studies around the lead compound revealed that introduction of electr ... | 2012 | 23960775 |
| culture proven newborn sepsis with a special emphasis on late onset sepsis caused by enterobacteriaceae in a level iii neonatal care unit in astana, kazakhstan. | newborn sepsis is one of the major public health concerns worldwide. also in our neonatal care unit, it is one of the major problems and especially infections with enterobacteriaceae were noted to pose an increasing problem in the last years. | 2013 | 23975158 |
| in vitro activity of ceftaroline-avibactam against gram-negative and gram-positive pathogens isolated from patients in canadian hospitals from 2010 to 2012: results from the canward surveillance study. | the in vitro activities of ceftaroline-avibactam, ceftaroline, and comparative agents were determined for a collection of bacterial pathogens frequently isolated from patients seeking care at 15 canadian hospitals from january 2010 to december 2012. in total, 9,758 isolates were tested by using the clinical and laboratory standards institute (clsi) broth microdilution method (document m07-a9, 2012), with mics interpreted by using clsi breakpoints (document m100-s23, 2013). ceftaroline-avibactam ... | 2013 | 23979759 |
| cu(ii), ni(ii), and zn(ii) complexes of salan-type ligand containing ester groups: synthesis, characterization, electrochemical properties, and in vitro biological activities. | a salen ligand on reduction and n-alkylation affords a novel [n2o2] chelating ligand containing ester groups [l = diethyl-2,2'-(propane-1,3-diylbis((2-hydroxy-3-methoxy benzyl)azanediyl))diacetate]. the purity of the ligand was confirmed by nmr and hplc chromatograms. its cu(ii), ni(ii), and zn(ii) complexes were synthesized and characterized by a combination of elemental analysis, ir, nmr, uv-vis, and mass spectral data, and thermogravimetric analysis (tg/dta). the magnetic moments, uv-vis, and ... | 2013 | 23983672 |
| heat resistance of histamine-producing bacteria in irradiated tuna loins. | consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ... | 2013 | 23992506 |
| a comparative study on phosphotransferase activity of acid phosphatases from raoultella planticola and enterobacter aerogenes on nucleosides, sugars, and related compounds. | natural and modified nucleoside-5'-monophosphates and their precursors are valuable compounds widely used in biochemical studies. bacterial nonspecific acid phosphatases (nsaps) are a group of enzymes involved in the hydrolysis of phosphoester bonds, and some of them exhibit phosphotransferase activity. nsap containing enterobacter aerogenes and raoultella planticola whole cells were evaluated in the phosphorylation of a wide range of nucleosides and nucleoside precursors using pyrophosphate as ... | 2014 | 23995227 |
| the rare sugar d-allose acts as a triggering molecule of rice defence via ros generation. | only d-allose, among various rare monosaccharides tested, induced resistance to xanthomonas oryzae pv. oryzae in susceptible rice leaves with defence responses: reactive oxygen species, lesion mimic formation, and pr-protein gene expression. these responses were suppressed by ascorbic acid or diphenylene iodonium. transgenic rice plants overexpressing osrbohc, encoding nadph oxidase, were enhanced in sensitivity to d-allose. d-allose-mediated defence responses were suppressed by the presence of ... | 2013 | 24014866 |
| isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (vigna unguiculata (l.) walp.) seedling growth. | the efficacy of four potential phosphate solubilizing enterobacter isolated from non-rhizospheric soil in western ghat forest in india. plant growth promoting ability of these isolates was evaluated in cowpea. all are gram negative, rod shaped, 0.8-1.6 mm in size, and psychrotrophic in nature, grow from 5 to 40°c (optimum temp. 28 ± 2°c). all isolates exhibits growth at a wide range of ph 6-12, optimum at ph 7.0 and tolerates up to 7% (w/v) salt concentration. 16s rrna gene sequencing reveals th ... | 2010 | 24026928 |
| antimicrobial ergosteroids and pyrrole derivatives from halotolerant aspergillus flocculosus pt05-1 cultured in a hypersaline medium. | in order to obtain more structurally novel and bioactive lead compounds for subsequent drug discovery, we have shifted the focus of our study from traditional microbial resources to 'extremophiles'. in this study, a halotolerant fungus aspergillus flocculosus pt05-1 was isolated from the sediment of putian saltern of fujian province of china in a hypersaline medium. two new compounds, (22r,23s)-epoxy-3β,11α,14β,16β-tetrahydroxyergosta-5,7-dien-12-one (1) and 6-(1h-pyrrol-2-yl)hexa-1,3,5-trienyl- ... | 2013 | 24030481 |
| horizontol dissemination of tem- and shv-typr beta-lactamase genes-carrying resistance plasmids amongst clonical isolates of enterobacteriaceae. | the extended-spectrum β-lactamase (esbl)-producing bacteria have been isolated at increasing frequency worldwide. expression of esbl is often associated with multidrug resistance and dissemination by resistance plasmids. during a two-month period in 2000, 133 clinical isolates of enterobacterial strains were randomly collected from outpatients and inpatients at a university hospital in turkey. the esbl producing strains were determined by double-disk synergy (dds) testing. twenty esbl producing ... | 2008 | 24031280 |
| mutations in the quinolone resistance-determining regions of gyra and parc in enterobacteriaceae isolates from brazil. | mutations in the quinolone resistance-determining regions (qrdr) in chromosomal gyra and parc genes and fluoroquinolone susceptibility profiles were investigated in quinolone-resistant enterobacteriaceae isolated from community and hospitalized patients in the brazilian southeast region. a total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to 2005 were investigated for mutations in the topoisomerases genes gyra and parc by amplifying and sequencing the qrdr region ... | 2012 | 24031957 |
| [the risk factors of ventilator-associated pneumonia in newborn and the changes of isolated pathogens]. | to study the risk factors of ventilator-associated pneumonia (vap) in newborn and the profiles of isolated pathogens. | 2013 | 24059113 |
| outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes. | to report the clinical features, management, and outcome of 7 cases of culture-proven multidrug-resistant enterobacter postoperative endophthalmitis following cataract surgery. | 2014 | 24063692 |
| development of a practical complete-kill assay to evaluate anti-acanthamoeba drugs. | acanthamoeba keratitis is a debilitating eye disease that requires effective topical drug therapy. currently, there is no standard in vitro test to evaluate anti-acanthamoeba drugs. | 2013 | 24077460 |
| irreproducible and uninterpretable polymyxin b mics for enterobacter cloacae and enterobacter aerogenes. | carbapenem-resistant enterobacter species are emerging nosocomial pathogens. as with most multidrug-resistant gram-negative pathogens, the polymyxins are often the only therapeutic option. in this study involving clinical isolates of e. cloacae and e. aerogenes, susceptibility testing methods with polymyxin b were analyzed. all isolates underwent testing by the broth microdilution (in duplicate) and agar dilution (in duplicate) methods, and select isolates were examined by the etest method. sele ... | 2013 | 24088860 |
| the presence of antibiotic-resistant nosocomial pathogens in endotracheal tube biofilms and corresponding surveillance cultures. | mechanically ventilated patients often develop ventilator-associated pneumonia (vap). soon after intubation, a mixed biofilm harboring microbial pathogens is formed on the endotracheal tube (et). it is believed that this biofilm contributes to the development of vap. unfortunately, the causative agent is often not known at the time vap is suspected, and early therapy often relies on the identification of surveillance cultures (sc). it is thus important to know whether these sc can predict the mi ... | 2013 | 24115610 |
| biodegradation of glycerol using bacterial isolates from soil under aerobic conditions. | glycerol, a non-biodegradable by-product during biodiesel production is a major concern to the emerging biodiesel industry. many microbes in natural environments have the ability to utilize glycerol as a sole carbon and energy source. the focus of this study was to screen for microorganisms from soil, capable of glycerol utilization and its conversion to value added products such as ethanol and 1,3-propanediol (1,3-pdo). twelve bacterial isolates were screened for glycerol utilization ability in ... | 2014 | 24117087 |
| avibactam reverts the ceftazidime mic90 of european gram-negative bacterial clinical isolates to the epidemiological cut-off value. | ceftazidime-avibactam consists of the beta-lactam ceftazidime and the novel non-beta-lactam beta-lactamase inhibitor avibactam. the effect of avibactam on the ceftazidime mic frequency distribution of gram-negative european clinical isolates was observed and compared to european committee for antimicrobial susceptibility testing (eucast) mic frequency distributions and epidemiological cut-off values (ecoffs) listed for ceftazidime. | 2014 | 24125508 |
| volatiles produced by soil-borne endophytic bacteria increase plant pathogen resistance and affect tritrophic interactions. | volatile organic compounds (vocs) released by soil microorganisms influence plant growth and pathogen resistance. yet, very little is known about their influence on herbivores and higher trophic levels. we studied the origin and role of a major bacterial voc, 2,3-butanediol (2,3-bd), on plant growth, pathogen and herbivore resistance, and the attraction of natural enemies in maize. one of the major contributors to 2,3-bd in the headspace of soil-grown maize seedlings was identified as enterobact ... | 2013 | 24127750 |
| infection due to colistin-resistant enterobacteriacae in critically-ill patients. | this study was conducted in response to the rising incidence of drug resistance observed in the intensive care unit (icu) of king fahad medical city. | 2013 | 24129623 |
| spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes. | an enhanced understanding of the metal ion binding and active site structural features of phosphoesterases such as the glycerophosphodiesterase from enterobacter aerogenes (gpdq), and the organophosphate degrading agent from agrobacterium radiobacter (opda) have important consequences for potential applications. coupled with investigations of the metalloenzymes, programs of study to synthesise and characterise model complexes based on these metalloenzymes can add to our understanding of structur ... | 2014 | 24135968 |
| antimicrobial efficacy and phytochemical analysis of indigofera trita linn. | an in vitro antimicrobial activity and phytochemical analysis of various extracts of indigofera trita l. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. a total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. petroleum ether extract showed moderate inhibitory activity against staphylococcus aureus (14.40 mm), ... | 2013 | 24146483 |
| epidemiology and molecular characterization of extended-spectrum beta-lactamase-producing enterobacter spp., pantoea agglomerans, and serratia marcescens isolates from a bulgarian hospital. | forty-two extended-spectrum beta-lactamase (esbl)-producing isolates of enterobacter aerogenes, enterobacter cloacae, pantoea agglomerans, and serratia marcescens, collected consecutively during the period january-november 2011 from the university hospital in varna, bulgaria, were studied to characterize their esbls by isoelectric focusing, group-specific pcr, and sequencing. the epidemiological relationship was evaluated by random amplified polymorphic dna analysis (rapd). transferability of es ... | 2014 | 24171449 |
| kpc-2 carbapenemase and dha-1 ampc determinants carried on the same plasmid in enterobacter aerogenes. | this study was conducted to analyse the presence of a plasmid-mediated carbapenem resistance mechanism in a clinical enterobacter aerogenes isolate from a patient from jiangsu province, people's republic of china. pcr and sequencing confirmed that the isolate harboured klebsiella pneumoniae carbapenemase (kpc)-2, dha-1 and tem-1 β-lactamase genes. both the kpc-2 and dha-1 genes were transferred to escherichia coli c600 by transconjugation, and southern blotting confirmed that these two genes wer ... | 2014 | 24173427 |
| co-fermentation of carbon sources by enterobacter aerogenes atcc 29007 to enhance the production of bioethanol. | we investigated the enhancement of bioethanol production in enterobacter aerogenes atcc 29007 by co-fermentation of carbon sources such as glycerol, glucose, galactose, sucrose, fructose, xylose, starch, mannitol and citric acid. biofuel production increases with increasing growth rate of microorganisms; that is why we investigated the optimal growth rate of e. aerogenes atcc 29007, using mixtures of different carbon sources with glycerol. e. aerogenes atcc 29007 was incubated in media containin ... | 2014 | 24185706 |
| absence of a role for lytic microorganisms in the decline of bacteria andsaccharomyces introduced into soil. | the populations ofklebsieila pneumoniae, escherichia coli, enterobacter aerogenes, andpseudomonas sp. fell following their addition to soil, but species lysing these gram-negative bacteria were not detected. the numbers ofstaphylococcus aureus andmicrococcus flavus fell by more than four orders of magnitude and ofsaccharomyces cerevisiae by more than two orders after their addition to soil. organisms lysing these gram-positive bacteria were present in soil, but their numbers did not increase as ... | 1987 | 24202607 |
| biodegradation of 2-methylquinoline by enterobacter aerogenes tj-d isolated from activated sludge. | bacterial strain enterobacter aerogenes tj-d capable of utilizing 2-methylquinoline as the sole carbon and energy source was isolated from acclimated activated sludge under denitrifying conditions. the ability to degrade 2-methylquinoline by e. aerogenes tj-d was investigated under denitrifying conditions. under optimal conditions of temperature (35 degrees c) and initial ph 7, 2-methylquinoline of 100 mg/l was degraded within 176 hr. the degradation of 2-methylquinoline by e. aerogenes tj-d cou ... | 2013 | 24218841 |
| experimental and modeling studies of a four-trophic level predator-prey system. | experimental studies of a microbial food chain involving organic carbon substrates,enterobacter aerogenes, and ciliate protozoansparamecium primaurelia anddldinium nasutum were conducted in stirred, aerated batch cultures. quantitative measurements were made of organic carbon levels and of cell numbers, mean cell volumes, and total biovolumes for all three microbial populations. a mathematical model based on monod kinetics was developed to describe this four-trophic level predator-prey system. t ... | 1982 | 24225890 |
| inhibitory effects of copper on bacteria related to the free ion concentration. | cu(2+) ion determinations were carried out in complex and in inorganic salts-glycerol media, to which increasing amounts of cu(ii) had been added, with the ion-specific cu(ii)-selectrode. likewise, complexing capacity of bacterial suspensions was estimated by titration with cuso4.copper-sensitive bacteria, e.g.,klebsiella aerogenes, were inhibited in their growth and survival in the range of 10(-8)-10(-6) m cu(2+) ion concentrations. in copper-buffered complex media, high copper loads could be t ... | 1979 | 24232421 |
| transport equations for a microbial predator-prey community. | a transport equation is used which describes the temporal behavior of interacting populations in changing environments. the formulation takes into account the internal state variables of the individuals. the general theory is applied to the transient analysis of a microbial predator-prey system using an approximate model for the specific cell growth rate and multigroup formulism to approximate the mass distribution within the population. experimental results in atetrahymena pyriformis- aerobacte ... | 1977 | 24233466 |
| synthesis, optimization, and characterization of silver nanoparticles from acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics. | the development of nontoxic methods of synthesizing nanoparticles is a major step in nanotechnology to allow their application in nanomedicine. the present study aims to biosynthesize silver nanoparticles (agnps) using a cell-free extract of acinetobacter spp. and evaluate their antibacterial activity. | 2013 | 24235826 |
| immobilization of the enzyme gpdq on magnetite nanoparticles for organophosphate pesticide bioremediation. | annually thousands of people die or suffer from organophosphate (pesticide) poisoning. in order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. we report here a ser127ala mutant based on the enzyme glycerophosphodiesterase (gpdq) from enterobacter aerogenes. the mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on pamam dendrimer-modified magnetite nanoparticles. the immobilized system was charac ... | 2014 | 24239906 |
| conservative management of severe bilateral emphysematous pyelonephritis: case series and review of literature. | emphysematous pyelonephritis (epn) is a life-threatening condition most commonly observed in diabetes, with nephrectomy believed to be the treatment of choice. however, nephrectomy in epn is associated with increased risk of complications secondary to associated hemodynamic instability and may result in lifelong hemodialysis in case of bilateral epn. we present three patients of severe bilateral epn and one patient of unilateral epn with diabetic ketoacidosis (dka) successfully managed conservat ... | 2013 | 24251204 |
| formiminoglutamase from trypanosoma cruzi is an arginase-like manganese metalloenzyme. | the crystal structure of formiminoglutamase from trypanosoma cruzi (tcfigase) is reported at 1.85 å resolution. although the structure of this enzyme was previously determined by the structural genomics of pathogenic protozoa consortium (pdb accession code 2a0m), this structure was determined at low ph and lacked bound metal ions; accordingly, the protein was simply annotated as "arginase superfamily protein" with undetermined function. we show that reconstitution of this protein with mn²⁺ confe ... | 2013 | 24261485 |
| hospital clonal dissemination of enterobacter aerogenes producing carbapenemase kpc-2 in a chinese teaching hospital. | carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant enterobacteriaceae. however, resistance to carbapenems has increased dramatically among enterobacteriaceae in our hospital. in this study, we report clonal dissemination caused by carbapenem-resistant enterobacter aerogenes (crea). in 2011, crea was identified from 12 patients admitted to the neurosurgical ward. all 12 clinical isolates were non-susceptible to cefotaxime, ceftazidim ... | 2014 | 24273320 |
| influence of azelaic and mandelic acid peels on sebum secretion in ageing women. | azelaic acid and mandelic acid are superficial peels commonly applied in people of various age groups. as they are mild and do not cause any side effects, they are also often used in elderly people. | 2013 | 24278065 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| biohydrogen production from sugarcane bagasse by integrating dark- and photo-fermentation. | hydrogen production from sugarcane bagasse (scb) by integrating dark-fermentation by enterobacter aerogenes mtcc 2822 and photo-fermentation by rhodopseudomonas bhu 01 was investigated. the scb was hydrolysed by sulphuric acid and the hydrolysate detoxified by passing through adsorbent resin column (amberlite xad-4) to remove the inhibitory furfural, and subjected to dark-fermentation. the cellulosic residue from acid hydrolysis was hydrolysed by the new isolate cellulomonas fimi to release suga ... | 2014 | 24291314 |
| molecular identification of aiia homologous gene from endophytic enterobacter species and in silico analysis of putative tertiary structure of ahl-lactonase. | the aiia homologous gene known to encode ahl- lactonase enzyme which hydrolyze the n-acylhomoserine lactone (ahl) quorum sensing signaling molecules produced by gram negative bacteria. in this study, the degradation of ahl molecules was determined by cell-free lysate of endophytic enterobacter species. the percentage of quorum quenching was confirmed and quantified by hplc method (p<0.0001). amplification and sequence blast analysis showed the presence of aiia homologous gene in endophytic enter ... | 2014 | 24309109 |
| hepatoblastoma in a 15-month-old female with trisomy 13. | trisomy 13 (t13) is a rare autosomal aneuploidy. greater than 90% of patients die during the first year of life. malignancies reported in association with t13 include two cases of wilms tumor and one case of pilocytic astrocytoma. there is no previous report of hepatoblastoma in patients with t13. we report a unique case of hepatoblastoma in a 15-month-old female with constitutional t13. our patient was born at 38 weeks gestation and was noted to have phenotypic features consistent with t13. gen ... | 2014 | 24311532 |
| risk factors and treatment outcomes of bloodstream infection caused by extended-spectrum cephalosporin-resistant enterobacter species in adults with cancer. | treatment of enterobacter infection is complicated due to its intrinsic resistance to cephalosporins. medical records of 192 adults with cancer who had enterobacter bacteremia were analyzed retrospectively to evaluate the risk factors for and the treatment outcomes in extended-spectrum cephalosporin (esc)-resistant enterobacter bacteremia in adults with cancer. the main outcome measure was 30-day mortality. of the 192 patients, 53 (27.6%) had bloodstream infections caused by esc-resistant entero ... | 2014 | 24321352 |
| cytotoxicity, mode of action and antibacterial activities of selected saudi arabian medicinal plants. | the flora of saudi arabia is one of the richest biodiversity areas in the arabian peninsula and comprises very important genetic resources of crop and medicinal plants. the present study was designed to investigate the cytotoxicity and the antibacterial activities of the organic extracts from twenty six saudi arabian medicinal plants. the study was also extended to the investigation of the effects of the extracts from the four best plants, ononis serrata (sy160), haplophyllum tuberculatum (sy177 ... | 2013 | 24330397 |
| the legionella pneumophila collagen-like protein mediates sedimentation, autoaggregation, and pathogen-phagocyte interactions. | although only partially understood, multicellular behavior is relatively common in bacterial pathogens. bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. for the waterborne pathogen legionella pneumophila, little is known about the roles of autoaggregation or the parameters which allow cell-cell interactions to occur. here, we determined the endogenous and exogenous factors sufficient to allow autoaggregation to take plac ... | 2014 | 24334670 |
| microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities. | this review gives an overview on the occurrence of sulfatases in prokaryota, eukaryota and archaea. the mechanism of enzymes acting with retention or inversion of configuration during sulfate ester hydrolysis is discussed taking two complementary examples. methods for the discovery of novel alkyl sulfatases are described by way of sequence-based search and enzyme induction. a comprehensive list of organisms with their respective substrate scope regarding prim- and sec-alkyl sulfate esters allows ... | 2013 | 24352732 |
| microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities. | this review gives an overview on the occurrence of sulfatases in prokaryota, eukaryota and archaea. the mechanism of enzymes acting with retention or inversion of configuration during sulfate ester hydrolysis is discussed taking two complementary examples. methods for the discovery of novel alkyl sulfatases are described by way of sequence-based search and enzyme induction. a comprehensive list of organisms with their respective substrate scope regarding prim- and sec-alkyl sulfate esters allows ... | 2013 | 24352732 |
| first detection and characterization of an oxa-48-producing enterobacter aerogenes isolate. | 2014 | 24355605 | |
| identification and characterization of sulfated carbohydrate-binding protein from lactobacillus reuteri. | we previously purified a putative sulfated-galactosylceramide (sulfatide)-binding protein with a molecular weight of 47 kda from the cell surface of lactobacillus reuteri jcm1081. the aim of this study was to identify the 47-kda protein, examine its binding to sulfated glycolipids and mucins, and evaluate its role in bacterial adhesion to mucosal surfaces. by cloning and sequencing analysis, the 47-kda protein was identified as elongation factor-tu (ef-tu). adhesion properties were examined usin ... | 2013 | 24391811 |
| inactivation of enterobacter aerogenes in reconstituted skim milk by high- and low-frequency ultrasound. | the inactivation of enterobacter aerogenes in skim milk using low-frequency (20khz) and high-frequency (850khz) ultrasonication was investigated. it was found that low-frequency acoustic cavitation resulted in lethal damage to e. aerogenes. the bacteria were more sensitive to ultrasound in water than in reconstituted skim milk having different protein concentrations. however, high-frequency ultrasound was not able to inactivate e. aerogenes in milk even when powers as high as 50w for 60min were ... | 2014 | 24394387 |
| biosynthesis of silver nanoparticles from marine seaweed sargassum cinereum and their antibacterial activity. | seaweed extracts of sargassum cinereum was used as a reducing agent in the eco-friendly extracellular synthesis of silver nanoparticles from an aqueous solution of silver nitrate (agno3). high conversion of silver ions to silver nanoparticles was achieved with a reaction temperature of 100(°) and a seaweed extract concentration of 10% with a residential time of 3 h. formation of silver nanoparticles was characterised by spectrophotometry and the scanning electron microscope. the average particle ... | 2013 | 24403664 |
| biosensing and bioremediation of cr(vi) by cell free extract of enterobacter aerogenes t2. | hexavalent chromium or cr(vi) enters the environment through several anthropogenic activities and it is highly toxic and carcinogenic. hence it is required to be detected and remediated from the environment. in this study, low-cost and environment-friendly methods of biosensing and bioremediation of cr(vi) have been proposed. crude cell free extract (cfe) of previously isolated enterobacter aerogenes t2 (gu265554; nii 1111) was prepared and exploited to develop a stable biosensor for direct esti ... | 2014 | 24410691 |
| microbiology of controlled rotting of egusi (colocynthis citrullus l.) fruits for the harvesting of the seeds. | microorganisms isolated from naturally rotting egusi fruits which are used as a source of dietary seeds, were mainly bacteria: bacillus subtilis, b. licheniformis, b. polymyxa, b. megaterium, b. pumilus, lactobacillus plantarum, la. brevis, leuconostoc mesenteroides, enterobacter aerogenes, e. cloacae, klebsiella aerogenes, k. pneumoniae, staphylococcus epidermidis, s. aureus, micrococcus and candida spp. the rotting period of egusi fruits could be decreased from 120 to 72 h by using a mixture o ... | 1994 | 24421184 |
| characterization of kpc-2-producing escherichia coli, citrobacter freundii, enterobacter cloacae, enterobacter aerogenes, and klebsiella oxytoca isolates from a chinese hospital. | twelve nonduplicated kpc-2-producing enterobacterial isolates, including three escherichia coli, two citrobacter freundii, two enterobacter cloacae, four enterobacter aerogenes, and one klebsiella oxytoca, were collected from various clinical samples within 18 months (march 2011 to september 2012). two of the 12 patients died from infections caused by kpc-2-producing pathogens, while the rest of the patients with kpc-2-producing pathogens improved or were cured. the majority of the clinical isol ... | 2014 | 24433026 |
| pleural effusion due to streptococcus milleri: case descriptions. | in this study we analyzed the characteristics of patients with pleural effusion secondary to streptococcus milleri studied retrospectively between january and march 2013 and found seven patients with a mean age of 60 years, 43% of which were smokers and 57% with a drinking habit. the most common associated factors were alcoholism, previous pneumonia and diabetes. other bacteria were identified as enterobacter aerogenes, bacteroides and prevotella intermedia capillosus in two patients. the mean d ... | 2014 | 24439468 |
| induction of lung cancer cell apoptosis through a p53 pathway by [6]-shogaol and its cysteine-conjugated metabolite m2. | dietary chemoprevention of cancer offers the possibility to suppress or inhibit cancer growth before it develops into more advanced and lethal stages. to this end, identification of novel compounds and their mechanisms of action is constantly needed. in this study, we describe that a major component of dry ginger (zingiber officinalis), [6]-shogaol (6s), can be quickly metabolized in a549 human lung cancer cell line. one of the resulting metabolites, the cysteine-conjugated 6s (m2), exhibits tox ... | 2014 | 24446736 |
| emergence of carbapenemaseproducing klebsiella pneumoniae of sequence type 258 in michigan, usa. | the prevalence of carbapenemase-producing enterobacteriaceae (cpe) in our hospital increased beginning in 2009. we aimed to study the clinical and molecular epidemiology of these emerging isolates. we performed a retrospective review of all adult patients with clinical cultures confirmed as cpe by positive modified hodge test from 5/2009- 5/2010 at the university of michigan health system (umhs). clinical information was obtained from electronic medical records. available cpe isolates were analy ... | 2013 | 24470956 |
| intraoperative vancomycin use in spinal surgery: single institution experience and microbial trends. | retrospective case series. | 2014 | 24480966 |
| reduction of meckelin leads to general loss of cilia, ciliary microtubule misalignment and distorted cell surface organization. | meckelin (mks3), a conserved protein linked to meckel syndrome, assists in the migration of centrioles to the cell surface for ciliogenesis. we explored for additional functions of mks3p using rna interference (rnai) and expression of flag epitope tagged protein in the ciliated protozoan paramecium tetraurelia. this cell has a highly organized cell surface with thousands of cilia and basal bodies that are grouped into one or two basal body units delineated by ridges. the highly systematized natu ... | 2014 | 24484742 |
| efficacy of commercial natural antimicrobials alone and in combinations against pathogenic and spoilage microorganisms. | microbial control strategies are needed in the food industry to prevent foodborne illnesses and outbreaks and prolong product shelf life. the aim of this study was to investigate and compare the efficacy of the commercial natural antimicrobials white mustard essential oil (wmeo), citrus flavonoid and acid blend (cfab), olive extract (oe), nisaplin (a compound containing nisin), and lauric arginate (lae) alone and in combinations against foodborne pathogens and spoilage microorganisms. mics of in ... | 2014 | 24490921 |
| autocrine/paracrine dopamine in the salivary glands of the blacklegged tick ixodes scapularis. | dopamine (da) is known to be the most potent activator of tick salivary secretion, which is an essential component of successful tick feeding. we examined the quantitative changes of catecholamines using a method coupling high-pressure liquid chromatography with electrochemical detection (hplc-ecd). we also investigated the levels of catecholamines conjugated to other molecules utilising appropriate methods to hydrolyse the conjugates. three different biological samples, salivary glands, syngang ... | 2014 | 24503219 |
| in vitro antibacterial efficacy of 21 indian timber-yielding plants against multidrug-resistant bacteria causing urinary tract infection. | to screen methanolic leaf extracts of 21 timber-yielding plants for antibacterial activity against nine species of uropathogenic bacteria isolated from clinical samples of a hospital (enterococcus faecalis, staphylococcus aureus, acinetobacter baumannii, citrobacter freundii, enterobacter aerogenes, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and pseudomonas aeruginosa). | 2013 | 24524024 |
| the presence of a aerogenes as indicator of faecal pollution. | 1951 | 24540291 | |
| [a study of the comparative activity of five antibiotics alone and in certain combinations against escherichia coli, aerobacter aerogenes pseudomonas aeruginosa and proteus sp]. | 1952 | 24542051 | |
| wound healing activity and mechanisms of action of an antibacterial protein from the venom of the eastern diamondback rattlesnake (crotalus adamanteus). | basic phospholipase a2 was identified from the venom of the eastern diamondback rattlesnake. the crotalus adamanteus toxin-ii (catx-ii) induced bactericidal effects (7.8 µg/ml) on staphylococcus aureus, while on burkholderia pseudomallei (khw), and enterobacter aerogenes were killed at 15.6 µg/ml. catx-ii caused pore formation and membrane damaging effects on the bacterial cell wall. catx-ii was not cytotoxic on lung (mrc-5), skin fibroblast (hepk) cells and in mice. catx-ii-treated mice showed ... | 2014 | 24551028 |
| kpc-3 carbapenemase harbored in fiik plasmid from klebsiella pneumoniae st512 and escherichia coli st43 in the same patient. | five carbapenem-resistant strains (three klebsiella pneumoniae, one escherichia coli, and one enterobacter aerogenes) were isolated between 2009 and 2012 at the verona university hospital, italy, during an epidemiological analysis of antibiotic resistance determinants and plasmid profiles in enterobacteriaceae. two out of the five strains, k. pneumoniae e530 and e. coli e558, were cultured from bile and abdominal drainage, respectively, of a single patient. the strains were resistant to beta-lac ... | 2014 | 24571419 |
| abiotic process for fe(ii) oxidation and green rust mineralization driven by a heterotrophic nitrate reducing bacteria (klebsiella mobilis). | green rusts (grs) are mixed fe(ii)-fe(iii) hydroxides with a high reactivity toward organic and inorganic pollutants. grs can be produced from ferric reducing or ferrous oxidizing bacterial activities. in this study, we investigated the capability of klebsiella mobilis to produce iron minerals in the presence of nitrate and ferrous iron. this bacterium is well-known to reduce nitrate using an organic carbon source as electron donor but is unable to enzymatically oxidize fe(ii) species. during in ... | 2014 | 24605878 |
| comparative genomic analysis of kpc-encoding pkpqil-like plasmids and their distribution in new jersey and new york hospitals. | the global spread of klebsiella pneumoniae carbapenemase (kpc) is predominately associated with k. pneumoniae strains genotyped as sequence type 258 (st258). the first st258-associated plasmid, pkpqil, was described in israel in 2006, but its history in the northeastern united states remains unknown. six pkpqil-like plasmids from four k. pneumoniae isolates (three st258 and one st234), one escherichia coli isolate, and one enterobacter aerogenes isolate, collected from 2003 to 2010 in new york ( ... | 2014 | 24614371 |
| carbapenemase-producing enterobacteriaceae in a tertiary hospital in madrid, spain: high percentage of colistin resistance among vim-1-producing klebsiella pneumoniae st11 isolates. | here we describe the carbapenemase genes, genetic relatedness and antimicrobial susceptibility data of 123 carbapenemase-producing enterobacteriaceae (cpe) clinical isolates recovered from 2010 to 2012, comprising klebsiella pneumoniae (n = 79), klebsiella oxytoca (n = 13), serratia marcescens (n = 14), enterobacter cloacae (n = 12), enterobacter asburiae (n = 4) and enterobacter aerogenes (n = 1). vim-1 was the most common carbapenemase (n = 101) followed by kpc-2 (n = 19), oxa-48 (n = 2) and i ... | 2014 | 24657043 |
| oral administration of bovine milk from cows hyperimmunized with intestinal bacterin stimulates lamina propria t lymphocytes to produce th1-biased cytokines in mice. | the goal of this study was to examine the effects of oral administration of bovine milk from cows hyperimmunized with a proprietary bacterin (immune milk "sustaina") on mucosal immunity in the intestine of adult mice. c57bl/6 mice were orally given immune or control milk for two weeks, and then lymphocyte population and the cytokine production in lamina propria of colon in normal mice and mice induced colitis by dextran sulphate sodium (dss) were detected. we found that the levels of ifn-γ and i ... | 2014 | 24686517 |
| habitat discrimination by gravid anopheles gambiae sensu lato--a push-pull system. | the non-random distribution of anopheline larvae in natural habitats suggests that gravid females discriminate between habitats of different quality. whilst physical and chemical cues used by culex and aedes vector mosquitoes for selecting an oviposition site have been extensively studied, those for anopheles remain poorly explored. here the habitat selection by anopheles gambiae sensu lato (s.l.), the principal african malaria vector, was investigated when presented with a choice of two infusio ... | 2014 | 24693951 |
| molecular characterization of esbl-producing enterobacteriaceae in northern portugal. | extended-spectrum β-lactamases (esbls) prevalence was studied in the north of portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and spain from a total of 7529 clinical strains. in the present study we recovered some members of enterobacteriaceae family, producing esbl enzymes, including escherichia coli (67.9%), klebsiella pneumoniae (30.6%), klebsiella oxytoca (0.5%), enterobacter aerogenes (0.5%), and citrobacter freundii (0.5%). β ... | 2014 | 24701189 |
| microwave-assisted synthesis of diverse pyrrolo[3,4-c]quinoline-1,3-diones and their antibacterial activities. | with the aim of developing a general and practical method for library production, a novel and efficient two-phase microwave-assisted cascade reaction between isatins and β-ketoamides in [bmim]bf4/toluene was developed for the synthesis of pyrrolo[3,4-c]quinoline-1,3-diones. the features of this methodology are, the use of microwave-assisted rapid synthesis, mild reaction conditions, high yields, operational simplicity, facile product separation, and recyclability. furthermore, the antibacterial ... | 2014 | 24749663 |
| an expanded set of fluorogenic sulfatase activity probes. | fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. here, we describe two fluorogenic enzyme probes, 3-o-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. the fluorogenic ... | 2014 | 24764280 |
| the first genotype determination of acanthamoeba potential threat to human health, isolated from natural water reservoirs in poland. | different species of amoebae belonging to the genus acanthamoeba are widely distributed in many parts of the world and known as free-living organisms. some strains of the protozoans may exist as parasites and cause risk to human health as causative agents of serious human diseases. currently, in poland, there is no sufficient information about the distribution of acanthamoeba strains and their genotypes in the environment. therefore, 20 environmental surface water samples were collected from dif ... | 2014 | 24770720 |
| health evaluation and survey of zoonotic pathogens in free-ranging capybaras (hydrochoerus hydrochaeris). | capybaras (hydrochoerus hydrochaeris) are the world's largest rodents and play an epidemiologic role in the transmission of zoonotic pathogens, including the causative agents of brazilian spotted fever, leptospirosis, and others. we surveyed the health of 31 free-ranging capybaras at the alberto löfgren state park, são paulo, brazil using a variety of diagnostic methods. hematology and serum chemistry were consistent with mild malnutrition and parasitism but did not indicate severe physiologic i ... | 2014 | 24779462 |
| in vitro susceptibility and distribution of beta-lactamases in enterobacteriaceae causing intra-abdominal infections in north america 2010-2011. | the study for monitoring antimicrobial resistance trends has been monitoring the activity of antimicrobials indicated for the treatment of intra-abdominal infections since 2004. this report documents the in vitro activity of several recommended antimicrobials against 3449 gram-negative bacilli isolated from the 30 and 25 participating sites in north america in 2010-2011, respectively, and characterizes the extended-spectrum beta-lactamases (esbl) identified in esbl-positive and ertapenem-non-sus ... | 2014 | 24813688 |
| inactivation of bacteria and yeast using high-frequency ultrasound treatment. | high-frequency (850 khz) ultrasound was used to inactivate bacteria and yeast at different growth phases under controlled temperature conditions. three species of bacteria, enterobacter aerogenes, bacillus subtilis and staphylococcus epidermidis as well as a yeast, aureobasidium pullulans were considered. the study shows that high-frequency ultrasound is highly efficient in inactivating the bacteria in both their exponential and stationary growth phases, and inactivation rates of more than 99% w ... | 2014 | 24835956 |
| molecular dynamics study of helicobacter pylori urease. | helicobacter pylori have been implicated in an array of gastrointestinal disorders including, but not limited to, gastric and duodenal ulcers and adenocarcinoma. this bacterium utilizes an enzyme, urease, to produce copious amounts of ammonia through urea hydrolysis in order to survive the harsh acidic conditions of the stomach. molecular dynamics (md) studies on the h. pylori urease enzyme have been employed in order to study structural features of this enzyme that may shed light on the hydroly ... | 2014 | 24839409 |
| a food additive with prebiotic properties of an α-d-glucan from lactobacillus plantarum dm5. | an α-d-glucan produced by lactobacillus plantarum dm5 was explored for in vitro prebiotic activities. glucan-dm5 demonstrated 21.6% solubility, 316.9% water holding capacity, 86.2% flocculation activity, 71.4% emulsification activity and a degradation temperature (td) of 292.2°c. glucan-dm5 exhibited lowest digestibility of 0.54% by artificial gastric juice, 0.21% by intestinal fluid and 0.32% by α-amylase whereas the standard prebiotic inulin, showed 25.23%, 5.97% and 19.13%, hydrolysis, respec ... | 2014 | 24857877 |
| biohydrogen and polyhydroxyalkanoate co-production by enterobacter aerogenes and rhodobacter sphaeroides from calophyllum inophyllum oil cake. | the feasibility of coupled biohydrogen and polyhydroxyalkanoate production by enterobacter aerogenes and rhodobacter sphaeroides using calophyllum inophyllum oil cake was studied under dark and photo fermentation conditions. the utilization of a non-edible acidic oil cake (c. inophyllum), and exploitation of a modified minimal salt media led to reduction in the cost of media. cost of fermentation is reduced by implementation of alternate dark-photo fermentative periods and through the use of a c ... | 2014 | 24859207 |
| dissemination of bacterial fluoroquinolone resistance in two multidrug-resistant enterobacteriaceae. | bacterial resistance to antimicrobials has become one of the greatest challenges for clinical microbiologists and healthcare practitioners worldwide. acquisition of resistance genes has proven to be difficult to characterize and is largely uncontrollable in the environment. here we sought to characterize conjugal horizontal gene transfer of plasmid-encoded fluoroquinolone resistance genes from two strains of enterobacteriaceae, one clinical and one from a municipal wastewater treatment plant env ... | 2014 | 24862457 |
| resistance determinants and mobile genetic elements of an ndm-1-encoding klebsiella pneumoniae strain. | multidrug-resistant enterobacteriaceae are emerging as a serious infectious disease challenge. these strains can accumulate many antibiotic resistance genes though horizontal transfer of genetic elements, those for β-lactamases being of particular concern. some β-lactamases are active on a broad spectrum of β-lactams including the last-resort carbapenems. the gene for the broad-spectrum and carbapenem-active metallo-β-lactamase ndm-1 is rapidly spreading. we present the complete genome of klebsi ... | 2014 | 24905728 |
| persistence of nosocomial bacteria on 2 biocidal fabrics based on silver under conditions of high relative humidity. | the survival of pathogenic microorganism on fabrics in the health care environment has a important role in nosocomial infections. the use of biocidal fabrics and surfaces could reduce the prevalence of the microorganisms in the hospital environment. | 2014 | 24930955 |
| incidence rates of carbapenemase-producing enterobacteriaceae clinical isolates in france: a prospective nationwide study in 2011-12. | to determine proportions and incidence rates of enterobacteriaceae producing carbapenemase among those non-susceptible (ns) to carbapenems in france. | 2014 | 24942333 |
| study of the role of anaerobic metabolism in succinate production by enterobacter aerogenes. | succinate is a core biochemical building block; optimizing succinate production from biomass by microbial fermentation is a focus of basic and applied biotechnology research. lowering ph in anaerobic succinate fermentation culture is a cost-effective and environmentally friendly approach to reducing the use of sub-raw materials such as alkali, which are needed for neutralization. to evaluate the potential of bacteria-based succinate fermentation under weak acidic (ph <6.2) and anaerobic conditio ... | 2014 | 24962116 |
| effect of vitreoscilla hemoglobin and culture conditions on production of bacterial l-asparaginase, an oncolytic enzyme. | l-asparaginase is a widely used cancer chemotherapy enzyme. the source for the enzyme with this property is mainly bacterial and its synthesis is strongly regulated by oxygen. in this study, we utilized two recombinant systems: one carried the gene (vgb) for the vitreoscilla hemoglobin (vhb), a protein of prokaryotic origin which confers a highly efficient oxygen uptake to its host and the other carried the l-asparaginase gene (ansb). the host bacteria were escherichia coli, enterobacter aerogen ... | 2014 | 24970046 |
| blood stream infections due to oxa-48-like carbapenemase-producing enterobacteriaceae: treatment and survival. | blood stream infections (bsis) due to carbapenem-resistant enterobacteriaceae (cre) are associated with high hospital mortality rates and present a tremendous challenge to clinicians. the optimal treatment remains undefined. we aimed to investigate the risk factors for mortality and the correlation between different treatment modalities and outcomes. | 2014 | 24998423 |
| re-examination of the taxonomic status of enterobacter helveticus, enterobacter pulveris and enterobacter turicensis as members of the genus cronobacter and their reclassification in the genera franconibacter gen. nov. and siccibacter gen. nov. as franconibacter helveticus comb. nov., franconibacter pulveris comb. nov. and siccibacter turicensis comb. nov., respectively. | recently, a taxonomical re-evaluation of the genus enterobacter, based on multi-locus sequence typing (mlst) analysis, has led to the proposal that the species enterobacter pulveris, enterobacter helveticus and enterobacter turicensis should be reclassified as novel species of the genus cronobacter. in the present work, new genome-scale analyses, including average nucleotide identity, genome-scale phylogeny and k-mer analysis, coupled with previously reported dna-dna hybridization values and bio ... | 2014 | 25028159 |
| screening and characterisation of antimicrobial properties of semisynthetic betulin derivatives. | betulin (lup-20(29)-ene-3β, 28-diol) is a naturally occurring triterpene, which is found in substantial amounts from the outer bark of birch trees. a library of 51 structurally diverse semisynthetic betulin derivatives was screened against five bacterial strains, enterobacter aerogenes, escherichia coli, enterococcus faecalis, pseudomonas aeruginosa, staphylococcus aureus and a fungal strain candida albicans, using broth microdilution assays. primary antimicrobial screening at 50 µm concentratio ... | 2014 | 25032708 |
| partial purification and characterization of a novel histidine decarboxylase from enterobacter aerogenes dl-1. | histidine decarboxylase (hdc) from enterobacter aerogenes dl-1 was purified in a three-step procedure involving ammonium sulfate precipitation, sephadex g-100, and deae-sepharose column chromatography. the partially purified enzyme showed a single protein band of 52.4 kd on sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the optimum ph for hdc activity was 6.5, and the enzyme was stable between ph 4 and 8. enterobacter aerogenes hdc had optimal activity at 40°c and retained ... | 2015 | 25036745 |
| chemical composition, antibacterial and antioxidant profile of essential oil from murraya koenigii (l.) leaves. | this study is designed to extract and examine chemical composition, antimicrobial and antioxidant activity of the hydro-distillated essential oil of murraya koenigii leaves from the south region of tamilnadu, india. matherials and methods: gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) analysis of the essential oil result was indicates the 33 different compounds representing 97.56 % of the total oil. | 2014 | 25050318 |
| microbiological features of kpc-producing enterobacter isolates identified in a u.s. hospital system. | microbiological data regarding klebsiella pneumoniae carbapenemase (kpc)-producing enterobacter spp. are scarce. in this study, 11 unique kpc-producing enterobacter isolates were identified among 44 ertapenem-nonsusceptible enterobacter isolates collected between 2009 and 2013 at a hospital system in western pennsylvania. all cases were healthcare-associated and occurred in medically complex patients. while pulsed-field gel electrophoresis showed diverse restriction patterns overall, multilocus ... | 2014 | 25053203 |
| distribution of free-living amoebae in a treatment system of textile industrial wastewater. | free-living amoebae have been found in soil, air and a variety of aquatic environments, but few studies have been conducted on industrial wastewater and none on wastewater from the textile industry. the aim of this study was to determine the presence and distribution of free-living amoebae in a biological treatment system that treats textile industrial wastewater. samples were taken from input, aeration tank, sedimentation tank and output. samples were centrifuged at 1200g for 15min, the sedimen ... | 2014 | 25072828 |
| multiplex real-time pcr probe-based for identification of strains producing: oxa48, vim, kpc and ndm. | the spread of multi-resistant enterobacteria, particularly carbapenem-resistant enterobacteriaceae (cre), in both community and hospital settings is a global problem. the phenotypic identification of cre is complex, occasionally inconclusive and time consuming. however, commercially available molecular assays are very expensive, and many do not allow the simultaneous identification of all genetic markers of resistance that have been recognised in cre (bla kpc, bla oxa-48, bla vim and bla ndm). t ... | 2014 | 25154795 |