Publications

transfer characteristics of triamterene and its analogs. central nervous system, placenta, and kidney.	earlier in vivo studies revealed a low concentration of triamterene in the brain of guinea pigs and baboons, and a transfer of the drug from the fetus to the mother. additional investigations have been performed to characterize further the transport system(s) for triamterene in the central nervous system (cns), placenta, and kidney. in guinea pigs a very low brain to free plasma concentration ratio (0.1) was achieved 3.5 min after drug administration and was maintained during 180 min of drug inf ...	1975	234832
influence of whole-plant barley reconstituted with sodium hydroxide on digestibility, rumen fluid and plasma metabolism of sheep.		1975	234930
gas and volatile fatty acid production at different rates of rumen microbial protein synthesis in vitro.		1975	234931
effects of methoxychlor and malathion on semen characteristics of rams.		1975	234932
effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen.	the influence oftemperature, addition of glycerol, initial freezing temperature, method of dilution, level of glycerol in the diluted semen, equilibration time and type of diluent on the survival and fertilizing capacity of deep-frozen according to the best conditions was compared with that of "fresh" semen. the addition of glycerol at plus30 degrees c resulted in a highly significant decrease in the mean proportion of motile spermatozoa immediately after thawing compared with the effect of addi ...	1975	235025
transfer processes in animal coats. i. radiative transfer.		1975	235133
transfer processes in animal coats. ii. conduction and convection.		1975	235134
transfer processes in animal coats. iii. water vapour diffusion.		1975	235135
investigation into diagnosis and treatment of cobalt deficiency in lambs.	the development of cobalt deficiency was studied in 30 scottish blackface lambs grazing pasture on a soil containing 0-17 ppm cobalt. by the end of an eight-week period 50 per cent of lambs were subjectively appraised as showing signs of cobalt deficiency. after a further period lasting four weeks, during which three groups of lambs were studied (one group, least affected by cobalt deficiency, acted as control, the second received a single cobalt bullet and the third received oral doses of 200 m ...	1975	235170
development of autonomic control of fetal circulation.	development of parasympathetic and sympathetic reflexes controlling heart rate, vascular pressures, and blood flows was investigated in fetal lambs weighing 300-5,800 g (65-165 days' gestation). cardiovascular responses to veratridine injections, atrial stretching, bilateral cervical vagotomy, and cholinergic blockade with atropine were used to test parasympathetic activities. responses to propranolol and phenoxybenzamine were used to test beta- and alpha-adrenergic activities. autonomic ganglio ...	1975	235216
effects of alveolar and perfusion hypoxia and hypercapnia on pulmonary vascular resistance in the lamb.	the effects of ventilatory hypoxia and hypercapnia and perfusion hypoxia and hypercapnia on pulmonary vascular resistance were studied in the intact lamb using right heart techniques to isolate and perfuse the left lower lobe. ventilatory hypoxia increased vascular resistance in the left lower lobe by constricting predominantly vessels upstream from small lobar veins, presumably small arteries. the response to hypoxia was not blocked by phentolamine and diphenhydramine in doses that markedly dec ...	1975	235217
puromycin does not inactivate the galactrosyltransferase of golgi membranes.		1975	235260
a method for the determination of sphingomyelinase activity.	a method is presented for the determination of the activity of sphingomyelinase. it is based on the determination of sphingomyelin before and after enzymatic conversion with the aid of a quantitative paper chromatographic analysis of phospholipids. this method is used for the determination of sphingomyelinase activity in various tissues such as those of the human brain and liver and fibroblast cultures. normal values are presented.	1975	235385
t lymphocyte function as the principal target of lymphocytic choriomeningitis virus-induced immunosuppression.	plaque-forming cell responses against sheep erythrocytes, escherichia coli lipopolysaccharide, pneumococcal polysaccharide, and polyvinylpyrrolidone were examined in mice infected with lymphocytic choriomeningitis virus. a 92 to 96 percent reduction of the thymus-dependent anti-sheep erythrocyte responses was observed 2 to 4 weeks after infection. however, the thymus-independent responses against the three other antigens were close to normal at all stages of the infetion. studies on allograft ...	1975	235488
the sorbitol-oxidizing enzyme of red blood cells.	the sorbitol-oxidizing enzyme of human erythrocytes has been partially purified and characterized. its kinetic characteristics were found to be similar to the sorbitol dehydrogenase of sheep liver and the dpn-xyluloxe dehydrogenase which have been isolated from sheep liver and guinea pig liver, respectively. although this enzyme is able to oxidize sorbitol relatively efficiently, it has very little activity toward dulcitol, and therefore cannot account for the high k-m pathway of methemoglobin r ...	1975	235592
effect of prolactin on androgen metabolism by the guinea-pig sex accessory organs.	the in vitro actions of prolactin on the metabolism of testosterone by the guinea-pig liver and sex accessory organs have been investigated under a variety of conditions including various concentrations of ovine or bovine prolactin, addition of reduced nicotinamide adenine dinucleotide phosphatate (nadph) or nicotinamide adenine dinucleotide (nad) to incubation media and various lengths of incubations. treatment of the epithelium and muscle of the guinea-pig seminal vesicle with nad elicited a s ...	1975	235637
role of amino groups in formation of human lymphocyte-xenogeneic erythrocyte rosettes; a proposed mechanism for antigen recognition.	formation of coulombic and possible hydrogen bonds between amino groups on human lymphocytes and negatively charged sites on sheep erythrocytes is involved in rosette formation. supportive evidence includes rosette inhibition by chemical binding of lymphocyte membrane amino groups, and the results of changing the ph, ionic concentration, and temperature of the reaction. although the possibility has not been excluded that the amino group dependence of this reaction is related to the property of c ...	1975	235762
a relationship between the molar proportion of propionic acid and the clearance rate of the liquid phase in the rumen of the sheep.	1. four rumen-cannulated sheep were given a forage mixture (f) of chopped hay-ground, pelleted, dried grass (92:8, w/w) and two concentrate mixtures (c and s) of ground barley-ground hay-flaked maize (46:24:30 and 56:24:20, by wt respectively) in twenty-four hourly meals each day. each of the diets was offered in successive periods of 16 d to give a feeding sequence f-s-c-s for one pair of sheep and c-s-f-s for the other pair. 2. the average composition (mol/100 mol) of the mixture of short-chai ...	1975	235941
survey of antibiotic residues in canadian slaughter animals.	kidneys and urine of cattle, swine, sheep and chickens were tested for bacterial growth inhibitors using bacillus subtilis and sarcina lutea as test organisms. results were as follows: 211 beef kidneys four positive, 611 swine kidneys five positive, 27 sheep and 120 chicken kidneys all negative, 2108 beef urine 76 positive, 2409 swine urine 186 positive, 176 sheep urine 17 positive. strongest reactions were obtained with b. subtilis on phosphate buffered ph 6.0 plates. larger zones were produced ...	1975	236082
specific suppression by anti-binding site antibody.		1975	236094
prolonged c3 depletion by cobra venom factor in thymus-deprived mice and its implication for the role of c3 as an essential second signal for b-cell triggering.	cobra venom factor (cof) is a potent immunogen in intact mice, and its capacity to deplete c3 is neutralized by antibody. in thymus-deprived (t times b) mice antibody was not elicited; c3 depletion by cof was prolonged, and subsequent injections were also effective. the effect of prolonged c3 depletion was examined on the antibody response to two t cell-independent immunogens, levan and siii. the spleen pfc responses to levan were unaffected by c3 depletion or thymus deprivation; those to siii w ...	1975	236257
diagnosis of schistosoma bovis infection in cattle by an indirect haemagglutination test.	an indirect haemagglutination test has been developed for the detection of schistosoma bovis antibody. fresh, formalin and glutaraldehyde fixed sheep erythrocytes as well as fresh and formalin fixed human erythrocytes were tested. tanned glutaraldehyde fixed sheep erythrocytes sensitized with an adult worm extract were the most sensitive and remained stable for at least 8 weeks at 4 degrees c. with such cells 98% of a group of s. bovis infected cattle were positive while negative results were ob ...	1975	236339
renal function in sheep during infusion of alkali metal ions into the renal artery.	1. the effect on renal function of 1 m solutions of licl, nacl, kcl, rbcl and cscl and 3 m-nacl infused close-arterially to the kidney for 10 min at 0-7ml./min has been studied in nine experiments on four unilaterally nephrectomized sheep. the levels of flow, electrolyte concentration and electrolyte excretion in the urine were measured before, during and for 50 min after the infusions. 2. the infusion of 1-m-nacl produced little change in urine flow and composition whereas 3 m-nacl resulted in ...	1975	236381
proceedings: plasma lh-pattens in cryptorchid rams and wethers.		1975	236388
two forms of guanylate cyclase in mammalian tissues and possible mechanisms for their regulation.	all mammalian tissues examined to date have two forms of guanylate cyclase with apparently different properties. from the studies in several laboratories, we suggest the following mechanisms for the possible regulation of guanylate cyclase activity: (1) factors that could alter the apparent cooperative nature of the enzyme, (2) interactions of metal ions with the substrate or enzyme, (3) factors that could overcome inhibition by atp, (4) mechanisms that could regulate the interconversion of late ...	1975	236425
l-fucose dehydrogenase from sheep liver.		1975	236435
6-phospho-d-gluconate dehydrogenase from sheep liver.		1975	236441
laryngeal chemosensitivity: a possible mechanism for sudden infant death.	in 32 anaesthetized piglets 1 to 42 days of age the distal trachea was cannulated and pressure changes were recorded. the proximal trachea was cannulated for introduction of test fluids into the laryngeal area. arterial pressure, heart rate, and central venous pressure were continuously recorded. arterial blood samples were obtained at intervals and analyzed for po2, pco2, ph, and hematocrit. normal saline produced no, or brief, transitory alterations of the respiratory pattern and arterial ...	1975	236536
placenta to lamb fetus transfusion in utero during acute hypoxia.	maternal and fetal hemodynamics, placental blood flow, fetal-placental blood volume, placental blood volume, and fetal blood volumes were measured in six chronic sheep prepartions to evaluate their changes during acute fetal hypoxia induced by maternal hypoxia. during fetal hypoxia, the maternal and fetal arterial blood pressure and heart rate were essentially unchanged. the placental blood flow (control equal to 325 ml.per kilogram per minute) was also unchanged during the hypoxia period. howev ...	1975	236656
uteroplacental blood flow during alkalosis in the sheep.	uteroplacental blood flow was measured by the radioactive-microsphere technique in eight near-term pregnant ewes during a normal control period and during maternal metabolic alkalosis. all measurements were made on awake, unanesthetized animals. alkalosis, defined for this study as an arterial ph of 7.60 or greater, was produced by the oral administration of sodium bicarbonate, 3 g/kg body wt. the rise in ph thus produced was unaccompanied by significant changes in systemic arterial blood pressu ...	1975	236672
steroid anesthetics.		1975	236704
ontogeny of thymus cell function.		1975	236719
studies of pituitary lactogenic hormone. physicochemical characterization on procine prolactin.		1975	236733
oxidation of oxyphenbutazone by sheep vesicular gland microsomes and lipoxygenase.		1975	236749
immunological effects of orchidectomy.	orchidectomy caused a non-specific increase of immunological reactivity in mice evidenced by accelerated rejection of skin allografts, increased response to oxazolone and sheep erythrocytes. these changes were associated with an increase of thymic size. the response to pneumococcal polysaccharide, which depends upon b-cells, was not increased. orchidectomised mice showed protection against induced methylcholanthrene sarcomas and transplantable tumours; tumours which are not obviously hormone dep ...	1975	236802
comparative birefringence of cornea.		1975	236894
reduced glutathione regeneration in glutathione-deficient erythrocytes from finnish landrace sheep.		1975	236951
biochemical properties of big renin extracted from human plasma.	the properties of big renin, a relatively inactive form of renin isolated from human plasma, were examined following partial purification by gel filtration. exposure of big renin to ph 3.0-3.6, or brief incubation with trypsin or pepsin, resulted in a ten-fold increase in enzymatic activity. activation was not effected by 4m nacl, 6m urea, or incubation with neuraminidase. both before and after inactivation, big renin eluted from sephadex gel more rapidly than normal plasma renin. during polyacr ...	1975	237015
the establishment of bacteroides ruminicola in a gnotobiotic lamb.		1975	237060
utilization of l-cystine by the gamma-glutamyl transpeptidase-gamma-glutamyl cyclotransferase pathway.	cystine is a good acceptor of the gamma-glutamyl group of gamma-glutamyl donors in the reaction catalyzed by gamma-glutamyl transpeptidase. the product of the enzymatic reaction and an authentic sample of gamma-glutamylcystine were shown to exhibit identical chromatographic and electrophoretic behaviors; acid hydrolysis gave equimolar amounts of cystine and glutamate. in studies with two gamma-glutamyl donors, apparent km values in the neighborhood of 0.3 mm were found for l-cystine; these value ...	1975	237267
the role of tyrosine transaminase in brain function.		1975	237299
effect of antithymocyte globulin pretreatment on immunological reconstitution of lethally irradiated animals.	clinical and experimental studies have shown that antithymocyte globulin pretreatment will reduce the severity of the graft-versus-host reaction after allogeneic bone marrow transplantation. to determine whether this was attributable to a persisting cytotoxic factor in the recipients' sera or a result of the "masking" of foreign antigens by the antihymocyte globulin, an experimental schema utilizing a syngeneic transfer of immunocompetent cells was devised. lethally irradiated mice that had been ...	1975	237349
retransplantation of human bone marrow. two immunosuppressive drug regimens.	a 15-year-old boy with aplastic anemia was successfully retransplanted with matched sibling bone marrow after failure of a first transplant from the same donor. cyclophosphamide was used as immunosuppression for the first transplant, and cyclophosphamide plus procarbazine plus antithymocyte globulin were used for the second transplant. laboratory studies of peripheral blood and bone marrow karyotypes and t and b lymphocytes supported the conclusion that immunosuppression was inadequate for the f ...	1975	237352
experimentally induced intestinal obstruction in sheep: paradoxical aciduria in metabolic alkalosis.	ewes with surgically prepared obstruction of the duodenum were used as a model in the study of ruminant metabolic alkalosis and paradoxical aciduria. metabolic alkalosis occurred as a result of irreversible chloride loss, presumably into the abomasum and forestomachs. analysis of data on serum and urinary electrolye concentrations provided limited support for the explanation of paradoxical aciduria in terms of chloride and potassium deficiencies. the accumulation undetermined anions in the serum ...	1975	237445
fluorescence studies of coenzyme binding to 6-phosphogluconate dehydrogenase.		1975	237488
physicochemical properties of erythropoietin: isoelectric focusing and molecular weight studies.		1975	237506
maternal and foetal effects of methoxyflurane anaesthesia in the pregnant ewe.	maternal and foetal cardiovascular, blood gas and acid-base changes were studied during 90 minutes of methoxyflurane anaesthesia. at 1.0 and 1.5 mac anaesthesia, despite slight to moderate falls in maternal blood pressure, cardiac output and uterine blood flow, no serious foetal deterioration was seen. 2.0 mac methoxyflurane was associated with marked falls in maternal blood pressure, cardiac output and uterine blood flow. foetal hypoxaemia and a mixed respiratory and metabolic acidosis develope ...	1975	237612
carboxylesterases (ec 3.1.1). a comparison of some kinetic properties of horse, sheep, chicken, pig, and ox liver carboxylesterases.	a comparative study of the kinetic be,avior of horse, sheep, chicken, pig, and ox liver carboxylesterases is reported. the enzymes exhibit similar specificites towards a series of phenyl esters in which the acyl group is varied, and towards a series of butyrate esters in which the alcohol group is varied. non-michaelis-menten kinetics are exhibited by the horse enzyme in the hydrolysis of methyl and ethyl butyrates, and by the pig enzyme with ethyl butyrate. each enzyme exhibits inhibition by on ...	1975	237618
elimination of suppressor t-cells in mice undergoing a graft-versus-host reaction expressed by increased response to polyvinylpyrrolidone (pvp).		1975	237632
comparative study of protein pattern of epididymal plasma of mouse, rat, rabbit and sheep.		1975	237731
periarteritis in experimental border disease of sheep. i. the occurrence and distribution of the lesion.		1975	237939
acid phosphatases activity in cheese and starters.	the acid phosphatase activity levels in a number of greek cheeses and in cheddar cheeses were found to be unaffected by storage for up to 18 months and 12 months respectively. in cheddar cheese, starter organisms made an insignificant contribution to this activity. studies of acid phosphatase prepared from streptococcus cremoris-lactis ncdo 762 starter cultures showed that the enzyme was of high molecular weight and largely particle-bound. the ph of optimum activity was 5-2 and the enzyme was in ...	1975	237943
release of corticotrophin releasing factor and other hypophysiotropic substances from isolated nerve-endings (synaptosomes).	synaptosomes isolated from the sheep and the rat hypothalamus contain corticotrophin releasing factor, vasopressin, prolactin-release inhibiting factor and probably all of the substances which participate in the regulation of adenohypophysial function. electrical field stimulation or depolarizing concentrations (55 mmol/l) of potassium ions cause a release of these factors from the incubated nerve-endings in a calcium-dependent manner. it is suggested that synaptosomes may provide a valuable app ...	1975	237973
effects of polyvalent anion binding to hemoglobin on oxygen and oxidation-reduction equilibria and their relevance to allosteric transition.		1975	238041
serologic evidence of venezuelan equine encephalitis in some wild and domestic populations of southern texas.	more than 2,500 sera from approximately 30 wild and domestic species in southern texas were tested for neutralizing antibodies to venezuelan equine encephalitis (vee). virus isolations were also attempted from blood and tissue samples of many of the wild specimens. vee neutralizing substances were present in a variety of species collected prior to the 1971 epizootic, suggesting that vee was present and perhaps enzootic in this area before the recent epizootic. serologic results of this study sug ...	1975	238050
the effect of sampling technique on acid-base balance and other blood parameters in the sheep.	the effect of 4 sampling routines--venipuncture, intravenous cannula, intravenous cannula following the administration of a tranquiliser (xylidino dihydrothiazine hydrochloride), intravenous cannula following exercise--were compared. blood ph and base excess values were similar after venipuncture and cannula sampling, but higher (p less than 0 with 05) after the administration of the tranquiliser and lower (p less than 3 with 05) following exercise. blood haemoglobin, haematocrit and lactate lev ...	1975	238060
the acute effects of intravenous infusion of parathyroid hormone on urine, plasma and saliva in the sheep.	following an intravenous loading injection of 75 u.s.p. units of para-thor-mone (eli lilly and co), seven conscious, non-pregnant, non-lactating merino ewes were infused with a maintenance dose of the hormone at a rate of 4-75 u.s.p. units/min for 2 hr. the classical hypercalcaemia and hypophosphataemia of the non-ruminant was observed, but the hypercalcaemi- was only small. plasma potassium concentration decreases, while there were no changes in plasma sodium, chloride or mangesium. the classic ...	1975	238258
[annual dynamics of acidobasic indices in the sheep blood].	altogether 218 sheep were followed for a year and the values of their acidobasic indices were examined, the follow-up comprises all seasons of the year, gravidity period, time of nursing and milk production. the actual ph value in the sheep blood varied from 7.30 to 7.48, with 7.40 average. the average value of partial co2 pressure amounted to 37.26 torr the individual values varying between 30.0 and 47.5 torr. baz excess varies from +5.0 to -6.3 mval/1 blood with year average of 1.11 mval/1 blo ...	1975	238330
[values of acido-basic balance in the blood of the beagle breed dog in comparison with the german shepherd dog].	the values of acidobasic balance were examined in 101 dogs of the beagle breed and in 20 german sheep-dogs. the beagle dogs were divided into five age groups. a difference was found between the values obtained in the group of the youngest dogs and those in older age groups. in comparison with beagle dogs, the values were higher in german sheep-dogs (ph, base excess). a figure is attached representing the deviations of the values of acidobasic balance of blood in the two breeds.	1975	238331
placental transport of alcohol and its effect on maternal and fetal acid-base balance.	the placental transport of alcohol and its effect on maternal and fetal acid-base balance studied in 10 sheep experiments by the maternal infusion of a 9.75 per cent solution of alcohol-dextrose at a rate of 15 c.c. per kilogram for 1 or 2 hours. serial maternal and fetal blood sampling during and following the alcohol infusion revealed rapid placental diffusion of alcohol, a highly significant correlation between maternal and fetal blood alcohol concentrations, and a similar peak concentration ...	1975	238397
changes in motility and ph in the digestive tract of experimentally overfed sheep.	motility of the digestive tract of 4 sheep was studied with radiotelemetric equipment. after base line records were made, each sheep was overfed with 70 g of grain per kilogram of body weight. the ruminoreticulum did not become static until the ingesta ph was less than 5. the cecum had the same patterns of motility and ph as did the ruminoreticulum, but these returned to normal more quickly in surviving sheep. the motility patterns of the abomasum and the small intestine were more erratic. resul ...	1975	238438
physiologic studies of experimentally grain-engorged cattle and sheep.	seven sheep weighing 34 to 41 kg, each, 3 steers and 1 heifer weighing 230 to 460 kg each, were experimentally, "overfed" (induced grain engorgement). the most significant changes occurred in ruminal ingesta ph, blood ph, packed cell volume (pcv), hemoglobin (hb), carbon dioxide pressure (pco2, total co2 (volume %), blood d-lactic acid, blood hco3, and base excess. there was no common denominator that was especially pathognomonic.	1975	238439
erythrocyte metabolism in normal and glutathione-deficient sheep.	glucose utilization and lactate formation in erythrocytes from normal and glutathione (gsh)-deficient sheep were similar. significant differences were observed, however, between the 2 groups of sheep in the production of 14-co2 from erythrocytes incubated with ascorbic acid or methylene blue, or both. the greater response of normal erythrocytes compared to erythrocytes deficient in gsh suggests that there are some metabolic differences in the pentose phosphate pathway (ppp) activity of the eryth ...	1975	238440
studies on stimulation and properties of antiglobulin antibodies. ii. antiglobulin antibodies against homologous globulins and antigens of thermally denatured autologous sera in the sera of rabbits immunized with various antigens.	sera of 48 rabbits after long-term immunization with suspensions of diplococcus pneumoniae, klebsiella friedländeri, proteus ox19 and solution of bovine albumin were examined for antibodies reacting with homologous gamma-globulins and antigens of thermally denatured autologous sera. appearance of antibodies of this type was related to duration of immunization, but not related to the type of antigen used. the experimental results were explained as a result of altered antigenic properties of antib ...	1975	238486
purification, properties and kinetics of sheep and human renin substrates.	sheep plasma renin substrate was purified 1,200-fold by using nephrectomised sheep plasma, followed by deae-sephadex chromatography and gel filtration. the purified substrate contained 8 mu-g angiotensin ii/mg protein and had an estimated molecular weight of 52,000. the kinetic characteristics of the purified substrate were identical both to those of unpurified nephrectomised sheep plasma and to normal sheep plasma substrates. at ph 7.5, k-m of the human renin-sheep substrate reaction was 0.29 m ...	1975	238499
studies on the immune response of congenitally athymic (nude) mice.	the central role of the thymus in immunity was assessed in nude mice. nudes failed to reject allografts and xenografts and to respond to foreign erythrocytes but responded normally to endotoxin and pneumococcal polysaccharide. thymus reconstitution was demonstrated in vivo and in vitro whereas reconstitution with thymic humoral factors or polyanions was not detected. coliform overgrowth and depressed iga levels in nudes appeared to contribute to wasting. these data emphasize the need for thymus ...	1975	238688
proceedings: adrenergic blockade and the pulmonary pressor response to lactic acid.		1975	238708
culture and morphological observations on trypanosoma melphagium.	the cultural characteristics of trypanosoma melophagium of sheep were studied. aspects investigated were size of the inoculum and population growth in modified monophasic medium for trypanosomes (mmmt), population growth in medium 199 with 10% inactivated calf serum containing 5, 10, and 15% hemolyzed defibrinated rabbit blood (199-cs-5, 199--cs-10, 199-cs-15) at 27 degrees c, effects on population growth of temperature and hydrogen ion concentration in mmmt, and morphology and morphometrics of ...	1975	238800
congenital defects of sheep.		1975	238924
effects of intravenous silica on immune and non-immune functions of the murine host.	silica, an agent toxic for macrophages, administered i.v. to dba/2 mice rapidly depresses the clearance of colloidal carbon by the reticuloendothelial system and reduces the in vitro phagocytic activity of peritoneal macrophages harvested 3 days after silica injection. silica blocks the humoral immune response to sheep erythrocytes and the cell-mediated immune response to allogeneic fibroblasts when given before antigen. silica also induces complex alterations in spleen cell responsiveness to co ...	1975	239057
the modifications of the final stages of the complement reaction by alkali metal cations.	we studied the effects of alkali metal cations on the terminal stages of complement lysis of human and sheep hk erythrocytes. sensitized erythrocytes (ea) were reacted with limited amounts of complement for 1 hr at 37 degrees c in buffer containing 147 mm nacl (na buffer), which resulted in 10-40% lysis. the unlysed cells were washed with na buffer at 0-2 degrees c and incubated for 1 hr at 37 degrees c in buffers containing 147 mm of the various alkali metal cations. although additional lysis ( ...	1975	239058
the development of a radioimmunoassay for cannabinoids in blood and urine.	antibodies, for use in radioimmunoassay, have been raised in sheep by immunization with a conjugate of delta9-tetrahydrocannabinol hemisuccinate and bovine serum albumin. antiserum titre and avidity were increased by successive booster doses of conjugate. the high degree of non-specific binding encountered in the radioimmunoassay of cannabinoids was reduced by the use of the solubilizing detergent triton x-405 and by restricting protein concentration in the assay medium. plasma samples were depr ...	1975	239149
association-dependent active folding of alpha and beta subunits of lutropin (luteinizing hormone).		1975	239249
maternal and fetal hemodynamic effects of diazoxide.	effects of diazoxide on systemic and uterine hemodynamics as well as on fetal circulation and blood respiratory gases were investigated in chronically instrumented pregnant sheep. diazoxide was administered intravenously either to the ewe or directly to the fetus in doses calculated on the basis of body weight. transfer of drug across placenta was also investigated. results showed that: a) when injected into the mother, there was consistent hypotensive effect with increased cardiac output and de ...	1975	239372
the role of cytochrome b5 in cytochrome p-450 enzymes.		1975	239544
new concepts in meat processing.		1975	239547
animal physiology and meat quality.		1975	239548
trypanosma (megatrypanum) melophagium: modes of attachment of parasites to mid-gut, hindgut and rectum of the sheep ked, melophagus ovinus.		1975	239553
the electrophoretic mobilities of 5-dimethylaminoaphthalene-1-suphonyl-glycopeptides and their relation molecular weight.	the relationship between the electrophoretic mobility at ph2.1 of dansyl-glycopeptides of known composition and their molecular weight is shown to conform with a model equation previously derived for peptides. a dansyl-glycopeptide prepared from hen's-egg ovotransferrin is degraded sequentially with two glycosidases. the molecular weight of each glycopeptide intermediate formed is determined from its electrophoretic mobility. from successive molecular-weight changes, the number and type of sugar ...	1975	239681
l-fucose metabolism in mammals. kinetic studies on pork liver 2-keto-3-deoxy-l-fuconate:nad+ oxidoreductase.	pork liver 2-keto-3-deoxy-l-fuconate:nad+ oxidoreductase has been shown to convert 2-keto-3-deoxy-l-fuconate to a 6-carbon acid tentatively identified as 2,4(or 5)-diketo-5(or 4)-monohydroxyhexanoate. the enzyme has a ph optimum of 10. 5 or higher. it is stabilized by dithiothereitol and inhibited by p-hydroxymercuribenzoate and heavy metals (ag+, hg2+, co2+, cd2+, pb2+, zn2+, and cu2+), suggesting the presence of a functionally essential sulfhydryl group; pre-treatment of enzyme with nad+ preve ...	1975	239939
[eeg-changes during preventive rabies therapy using the hempt-vaccine].		1975	240124
oxygen-linked co2 transport in sheep blood.	we have analyzed oxygen-linked carbamate formation in sheep hemoglobin b by measuring a) the effect of co2 on oxygen affinity and bohr effect in red cell suspensions and dilute (1.3 mm hb4) and concentrated (5 mm hb4) hemoglobin solutions at 37 degrees c and b) co2 binding curves of deoxygenated and oxygenated whole blood and hemoglobin solutions, respectively, at the same temperature. in the presence of co2 both the bohr effect and oxygen affinity were significantly lower in 1.3-mm hb4 solution ...	1975	240281
blood oxygen affinity and alveolar ventilation in relation in body weight in mammals.	the validity of the concept relating blood oxygen affinity and alveolar ventilation to body weight in homeothermic mammals was reexamined with blood ph used as the fixed variable. blood po2 at 50% saturation at ph 7.4 (p50(7.4)) and pco2 of oxygenated blood at ph 7.4 (pco2(7.4)) from a variety of homeothermic mammals were determined at 37 degrees c by in vitro equilibration techniques. in some species, arterial pco2, po2, and ph were also measured. pco2(7.4), which was similar to arterial pco2, ...	1975	240284
effects of dietary hydrochloric acid on voluntary food intake and metabolism of sheep in relation to the use of mineral acids as silage additives.	1. in expt i, a pelleted grass-meal diet was supplemented with hydrochloric acid, added to the grass pellets before feeding, at five levels from 0 to 628 mmol/kg dry matter (dm). each diet was offered ad lib. for 21 d to five sheep in a 5 x 5 latin-square design. 2. voluntary food intake decreased rectilinearly with increasing hcl supplementation (p less than 0.001), to 42% of the control value for sheep on the high-hcl diet. the decrease in food intake was related both to dietary ph and to the ...	1975	240379
a study of the effectiveness of calcined magnesite as a neutralizing agent for hydrochloric acid in the diet of sheep.	1. five experimental diets were offered ad lib. for 21 d to five sheep in a 5 x 5 latin-square design experiment as follows: pelleted grass meal alone (control); pelleted grass meal plus hydrochloric acid (470 mmol/kg dry matter (om); pelleted grass meal plus 470 mmol hcl/kg dm and either an equivalent amount of calcined magnesite (mgo) (235 mmol/kg dm) or twice the amount (470 mmol mgo/kg dm); pelleted grass meal plus 470 mmol mgo/kg dm. 2. mgo supplementation partly prevented the reduction in ...	1975	240380
purification, properties and glycoprotein nature of arylsulfatase a from sheep brain.	a simple and rapid method for the purification of arylsulfatase a (ec 3.1.6.1) from sheep brain has been developed. this includes the concanavalin a-sepharose affinity chromatography and the ph-dependent polymerization and depolymerization of the enzyme. by these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. sheep brain arylsulfatase a has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. the constituent neutral sugars were iden ...	1975	240423
systemic and pulmonary hemodynamic responses to adrenergic and cholinergic agonists during fetal development.	systemic and pulmonary hemodynamic responses to adrenergic and cholinergic agonists were investigated in fetal lambs between 60 days and term gestation. the cardiovascular response to these agents increases with fetal age, and the increase is related to maturation of the effector system rather than the vascular receptors. the fetal pulmonary vascular bed and the ductus arteriosus are the primary components responding to acetylcholine; the systemic response is secondary to the occuring in the lun ...	1975	240447
water transport response of toad bladder to prolactin.		1975	240570
ion and water balance in the feeding lone star tick.		1975	240578
measurement of the reflectances for solar radiation of the coats of live animals.		1975	240591
effects of adrenergic drugs on sheep oviduct motility.	recordings of electrical activity of the oviduct in conscious ewes fitted with an extracellular multi-electrode assembly confirm the presence of alpha- and beta-adrenergic receptors in both the ampullary and isthmic portions. regional differences in response to noradrenaline and isoprenaline were observed particularly during oestrus. in all cases the lowest threshold of sensitivity was exhibited by the isthmic portion of the oviduct.	1975	240729
duodenal acidification: stimulus for the release of intestinal hormones in sheep.		1975	240791
secondary igg responses to type iii pneumococcal polysaccharide. i. kinetics and antigen requirements.	mice primed with a thymus- (t)3 dependent form of type iii pneumococcal polysaccharide (s3), i.e., s3 coupled to sheep or horse erythrocytes (s3-rbc), produce s3-specific igg antibody after secondary challenge with either the t-dependent (s3-rbc) or t-independent (s3) form of the antigen. the potential to produce igg antibody after challenge with s3-rbc appears earlier after priming than the potential to produce igg after challenge with s3, suggesting that different "memory" cells may be involve ...	1975	240888
functional arginyl residues as atp binding sites of glutamine synthetase and carbamyl phosphate synthetase.	the reaction of phenylglyoxal with two enzymes in which atp plays a complex role has been studied. both ovine brain glutamine synthetase and escherichia coli carbamyl phosphate synthetase [carbamoyl-phosphate synthase (glutamine); atp:carbamate phosphotransferase (dephosphorylating, amido-transferring); ec 2.7.2.9]were inactivated by phenylglyoxal. the specificity of this reagent for arginyl residues of the two proteins was confirmed by amino acid analysis. atp, but not the other substrates, pro ...	1975	241076
[occurrence, diagnosis and course of chronic hepatitis in weaned lambs of a flock of texel sheep (author's transl)].	a disease of weaned lambs is described, which is attributable to chronic hepatitis marked by impaired secretion of cholephils in the acute or early stage and by inadequate growth due to persistent dysfunction of the liver during the subsequent or chronic stage. regardless of its duration, the disease may be diagnosed in the living animal by determining the sdh and gammagt activity levels, the total serum protein, the serum iron concentration and the serum iron-binding capacity.	1975	241130
chimaerism of immunocompetent cells in allogeneic bone marrow-reconstituted lethally irradiated chickens.	injection of parental bone marrow cells into 12-day-old lethally irradiated f1 hybrid chickens resulted in chimaerism of donor-type graft-versus-host (gvh)-reactive cells and suppression of antisheep red blood cell antibody response. these manifestations of a chronic graft-versus-host reaction were prevented by pretreatment of the donor marrow with specific anti-t cell globulin. in some chimaeras donor-type gvh-reactive cells developed gradually from t cells precursors of donor origin. transplan ...	1975	241144
the mutagenic effect of measles virus on cell cultures of different origin.	differences between strains of measles virus in their mutagenic effect on cells of different animals and man were found. the mutagenic activity of measles virus strains was species-specific. in the process of spontaneous transformation the sensitivity of cells to the mutagen changed.	1975	241227
streptovirudins -- new antibiotics with antiviral activity. the antiviral spectrum and inhibition of newcastle disease virus in cell cultures.	streptovirudins are new antibiotics isolated as a mixture of several structurally related compounds from fermentations of streptomyces griseoflavus (krainsky) waksman et henrici var. thuringensis ja 10124. they possess antiviral activity against rna and dna viruses cultivated in chick embryo cells, namely sindbis, fowl plague, newcastle disease (ndv), pseudorabies, vaccinia and sheep abortion viruses. the naturally formed streptovirudin complex, in concentrations of 20-2.5 mug/ml inhibited the v ...	1975	241229
[benign neurologic accident following anti-rabies vaccination. unforeseen clinical and biological aspects].	a minor and benign encephalitogenic reactions was observed in the days following administration of suckling mouse brain anti-rabies vaccine. the syndrome appeared at the 18th day of the treatment, undertaken according to gamet and atanasiu's shortened treatment schedule. recovery occured without sequellae after 5 days of corticosteroid therapy. compared to the reports of the literature, the benignity of the central nervous system involvement was unexpected. three immunological problems are stres ...	1975	241305
flow properties of microcapsule suspensions as a model of blood.		1975	241441