Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the complete mitochondrial genome of orientobilharzia turkestanicum supports its affinity with african schistosoma spp. | orientobilharzia turkestanicum is a blood fluke of many mammals and causes orientobilharziasis that is also a neglected parasitic zoonosis because the cercaria of o. turkestanicum can infect humans and cause cercarial dermatitis. the present study determined the complete sequence of mt genome of o. turkestanicum and revised its phylogenetic position based on mt gene content and arrangement. the complete mtdna sequence of o. turkestanicum was 14,755 bp in length, which is slightly larger than the ... | 2011 | 21930247 |
| Awareness and knowledge of schistosomiasis infection and prevention in the "Three Gorges Dam" reservoir area: a cross-sectional study on local residents and health personnel. | Schistosomiasis is a severe public health problem in China. It has been predicted that the ecological changes caused by the "Three Gorges Dam", the world's largest hydropower project, could potentially aggravate the spread of schistosomiasis in the area. This study focused on investigating (a) local residents' knowledge on the potential risks of schistosomiasis and (b) the capability of local health personnel in preventing schistosomiasis. | 2011 | 21945272 |
| clinics in diagnostic imaging (136). chronic schistosoma japonica infection of the liver. | a 37-year-old man from china was initially seen at the urology clinic for symptoms of urinary colic. plain computed tomography (ct) imaging of the kidneys, ureters and bladder was performed, which (in addition to demonstrating renal calculi) revealed incidental findings of 'turtleback' septal and capsular calcifications, features pathognomonic for schistosomiasis japonica. other classical features were demonstrated on the triphasic hepatic ct imaging that was subsequently performed. the clinical ... | 2011 | 21947150 |
| identification and characterization of six novel tetraspanins from schistosoma japonicum. | tetraspanins (tsps), also known as members of the trans-membrane 4 super-family (tm4sf), comprise an assemblage of surface antigens reported in eukaryotic organisms. in the work presented here, six novel tsp proteins from the human blood fluke schistosoma japonicum (s. japonicum) were produced and analyzed through a combination of bioinformatics and experimental approaches. | 2011 | 21958506 |
| identification, structure and function of a novel tetraspaninhomologue from spirometra erinaceieuropaei. | to identify a full length c dna sequence of a novel tetraspanin (tsp) homologue from spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods. | 2011 | 21967699 |
| [cloning, expression and stage-specific analysis of schistosoma japonicum p7 antigen and evaluation of its value in early diagnosis]. | to clone and express schistosoma japonicum p7 antigen (genbank accession no. eu121231), analyze stage-specific transcription and expression of the antigen, and evaluate its value in early diagnosis. | 2011 | 21970101 |
| [cloning and expression of adenine phosphoribosyltransferase (aprt) and its differential expression analysis during the developmental stages of schistosoma japonicum]. | to clone and express adenine phosphoribosyltransferase gene of schistosoma japonicum, and analyze its stage-specific transcription and expression at different developmental stages of s. japonicum. | 2011 | 21970105 |
| [Anti-hepatofibrosis effect of fasudil hydrochloride on Schistosoma japonicum-infected mice]. | To investigate the anti-fibrotic effect of fasudil hydrochloride on Schistosoma japonicum-infected mice, and the effect of fasudil hydrochloride on hepatic stellate cells (HSCs). | 2011 | 21970106 |
| [serological detection of cryptosporidium spp. infection in outpatients in changchun]. | cp23 gene of cryptosporidium parvum was expressed in escherichia coli, and the recombinant protein was purified. its immunoreactivity was analyzed by western blotting. serum samples were collected from outpatients of different ages from august to november, 2010 in changchun. indirect elisa was established to detect the anti-cp23 igg in sera. western blotting analysis indicated that the recombinant cp23 protein was recognized by sera from cryptosporidium panum-infected calves and positive human s ... | 2011 | 21970120 |
| [preliminary study on the immunological characteristics of permissive and non-permissive hosts infected with schistosoma japonicum]. | to study the difference among immune responses of three kinds of experimental animals with different susceptibility to the infection of schistosoma japonicum, and preliminarily explore the mechanism of the immune response in permissive and non-permissive hosts. | 2011 | 21972600 |
| [Effect of epitope-based peptide-DNA dual vaccines against Schistosoma japonicum in mice]. | A C-T-B PDDV mixture of the three constructed epitope-based peptide-DNA dual vaccines (PDDV) containing the CTL (C), Th (T) and B-cell (B) epitopes from Sj22.6 tegument (C-PDDV, T-PDDV and B-PDDV) with a 1:1:1 ratio was prepared. Thirty-six mice were randomly divided into six groups averagely named as 18K group, PBS group, C-PDDV group, T-PDDV group, B-PDDV group, and C-T-B PDDV group. All the mice received three immunizations at 2-week intervals with the same dose of antigen (10 microg DNA+28 m ... | 2011 | 21972614 |
| The cell biology of schistosomes: a window on the evolution of the early metazoa. | This review of schistosome cell biology has a dual purpose; its intent is to alert two separate research communities to the activities of the other. Schistosomes are by far and away the best-characterised platyhelminths, due to their medical and economic importance, but seem to be almost totally ignored by researchers on the free-living lower metazoans. Equally, in their enthusiasm for the parasitic way of life, schistosome researchers seldom pay attention to the work on free-living animals that ... | 2011 | 21976269 |
| Human antibody response to thioredoxin peroxidase-1 and tandem repeat proteins as immunodiagnostic antigen candidates for Schistosoma japonicum infection. | Schistosomiasis continues to be a public health problem in many tropical and subtropical countries. Improving the diagnostic tools for surveillance and monitoring in areas that have reached elimination level will help hasten the possible elimination of this disease. This study therefore aims to develop enzyme-linked immunosorbent assay through the use of recombinant proteins such as thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, and Sj7TR). Cutoff values ... | 2011 | 21976571 |
| upregulated expression of cytotoxicity-related genes in ifn-γ knockout mice with schistosoma japonicum infection. | it is well accepted that ifn-γ is important to the development of acquired resistance against murine schistosomiasis. however, the in vivo role of this immunoregulatory cytokine in helminth infection needs to be further investigated. in this study, parasite burden and host immune response were observed in ifn-γ knockout mice (ifng ko) infected with schistosoma japonicum for 6 weeks. the results suggested that deficiency in ifn-γ led to decreased egg burden in mice, with low schistosome-specific ... | 2011 | 21976969 |
| proteomic analysis of human skin treated with larval schistosome peptidases reveals distinct invasion strategies among species of blood flukes. | skin invasion is the initial step in infection of the human host by schistosome blood flukes. schistosome larvae have the remarkable ability to overcome the physical and biochemical barriers present in skin in the absence of any mechanical trauma. while a serine peptidase with activity against insoluble elastin appears to be essential for this process in one species of schistosomes, schistosoma mansoni, it is unknown whether other schistosome species use the same peptidase to facilitate entry in ... | 2011 | 21980548 |
| anthelmintic effect of artesunate in experimental heterophyid infection. | artesunate is a water-soluble semi-synthetic drug derived from artemisinin which is extracted from leaves of the chinese plant artemisia annua. this successful basically-antimalarial drug has been proved to be also effective against trematodes like schistosoma japonicum, s. mansoni, fasciola hepatica and clonorchis sinensis. the present work aimed to investigate the in-vivo efficacy of artesunate against heterophyids in mice, using praziquantel as a therapeutic control. results showed that artes ... | 2011 | 21980784 |
| a diagnostic challenge for schistosomiasis japonica in china: consequences on praziquantel-based morbidity control. | worldwide schistosomiasis continues to be a serious public health problem. over the past five decades, china has made remarkable progress in reducing schistosoma japonicum infections in humans to a relatively low level. endemic regions are currently circumscribed in certain core areas where re-infection and repeated chemotherapy are frequent. at present, selective chemotherapy with praziquantel is one of the main strategies in china's national schistosomiasis control program, and thus diagnosis ... | 2011 | 21981948 |
| Effect of the in vivo activity of dihydroartemisinin against Schistosoma mansoni infection in mice. | Dihydroartemisinin, formerly known as an antimalarial drug, is the main metabolite of the mother compound artemisinins, as well as of artemether and artesunate. It has been shown that the drug exhibits antischistosomal efficacy against Schistosoma japonicum. The purpose of the current study was to assess the in vivo effect of dihydroartemisinin against Schistosoma mansoni infection in mice. Drugs at a single oral dose of 300 mg/kg were given to mice to assess the efficacy against different devel ... | 2011 | 22006193 |
| insights into the functional biology of schistosomes. | the need to discover new treatments for human schistosomiasis has been an important driver for molecular research on schistosomes, a major breakthrough being the publication of the schistosoma mansoni and schistosoma japonicum genomes in 2009. this 'primer' considers recent advances in the understanding of schistosome biology by providing a snapshot of selected areas of contemporary functional schistosome research, including that on the genome, the tegument, cell signalling and developmental bio ... | 2011 | 22013990 |
| Suppression of adaptive immunity to heterologous antigens by Sj16 of Schistosoma japonicum. | Abstract Despite great effort has been given to control the disease, schistosomiasis remains the most important human helminth infection in terms of morbidity and mortality. Natural infection of schistosomes induces very little protective immunity against reinfection. Moreover, effective schistosomiasis vaccines for practical use have not been developed. Schistosomes appear to have evolved highly effective modulatory mechanisms on their host's immune system that promote the parasites' survival ... | 2011 | 22017401 |
| delayed tail loss during the invasion of mouse skin by cercariae of schistosoma japonicum. | summarya traditional assumption is that schistosome cercariae lose their tails at the onset of penetration. it has, however, recently been demonstrated that, for schistosoma mansoni, cercarial tails were not invariably being shed as penetration took place and a high proportion of tails entered human skin under experimental conditions. this phenomenon was termed delayed tail loss (dtl). in this paper, we report that dtl also happens with s. japonicum cercariae during penetration of mouse skin. it ... | 2011 | 22018291 |
| Polyinosinic-polycytidylic acid attenuates hepatic fibrosis in C57BL/6 mice with Schistosoma japonicum infection. | The development of hepatic fibrosis is the principal cause of morbidity and mortality in human beings infected with schistosoma. In this study, we investigated the effect of polyinosinic-polycytidylic acid (poly I:C) on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. S. japonicum cercariae infected mice were injected with poly I:C at the onset of egg granuloma formation (early phase poly I:C treatment) or after the formation of liver fibrosis (late phase poly I:C treatment). Our ... | 2012 | 22023732 |
| Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata. | Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L3). However, the parasite is difficult to identify in snails. The purpose of this study was to develop a quick, simple molecular method to survey for A. cantonensis in intermediate host snails. | 2011 | 22023992 |
| stirred, not shaken: genetic structure of the intermediate snail host oncomelania hupensis robertsoni in an historically endemic schistosomiasis area. | oncomelania hupensis robertsoni is the sole intermediate host for schistosoma japonicum in western china. given the close co-evolutionary relationships between snail host and parasite, there is interest in understanding the distribution of distinct snail phylogroups as well as regional population structures. therefore, this study focuses on these aspects in a re-emergent schistosomiasis area known to harbour representatives of two phylogroups - the deyang-mianyang area in sichuan province, china ... | 2011 | 22029536 |
| identification of in vivo protein phosphorylation sites in human pathogen schistosoma japonicum by a phosphoproteomic approach. | schistosome is the causative agent of human schistosomiasis and related animal disease. reversible protein phosphorylation plays a key role in signaling processing that are vital for a cell and organism. however, it remains to be undercharacterized in schistosomes. in the present study, we characterized in vivo protein phosphorylation events in different developmental stages (schistosomula and adult worms) of schistosoma japonicum by using microvolume immobilized metal-ion affinity chromatograph ... | 2012 | 22036931 |
| Interleukin-4 (IL-4) and IL-13 Suppress Excessive Neutrophil Infiltration and Hepatocyte Damage during Acute Murine Schistosomiasis Japonica. | Due to the importance of neutrophils and proinflammatory cytokines in schistosomal liver damage, we analyzed the mechanisms underlying neutrophil and proinflammatory responses in murine schistosomiasis japonica. We found that granulomatous inflammation around parasite eggs in the liver was greater in Schistosoma japonicum-infected IL-4(-/-) IL-13(-/-) (double-knockout [DKO]) mice than in infected wild-type (WT) mice at 6 weeks, but not at 8 weeks, postinfection, suggesting the importance of Th2 ... | 2012 | 22038918 |
| toward sustainable and comprehensive control of schistosomiasis in china: lessons from sichuan. | triggered by a fascinating publication in the new england journal of medicine detailing china's new multi-pronged strategy to control and eventually interrupt the transmission of schistosoma japonicum, this plos neglected tropical diseases debate critically examines the generalizability and financial costs of the studies presented from the marshlands of the lake region. edmund seto from the university of california and colleagues emphasize that the epidemiology and control of schistosomiasis var ... | 2011 | 22039563 |
| A strategy for emergency treatment of Schistosoma japonicum-infested water. | Schistosomiasis japonica, caused by contact with Schistosoma japonicum cercaria-infested water when washing, bathing or production, remains a major public-health concern in China. The purpose of the present study was to investigate the effect of a suspension concentrate of niclosamide (SCN) on killing cercaria of S. japonicum that float on the water surface, and its toxicity to fish, so as to establish an emergency-treatment intervention for rapidly killing cercaria and eliminating water infecti ... | 2011 | 22047607 |
| neuroprotective potential beyond immunoregulation of helminth infection as a therapeutic target in multiple sclerosis. | multiple sclerosis (ms) is a common disabling autoimmune disease in young adults which does not have an effective treatment. the prevalence of immune-mediated diseases is higher in developed countries with hygienic environments, suggesting that helminthic infection may protect from autoimmune diseases. previously, we reported that soluble egg antigens (sea) from schistosoma japonicum suppressed experimental autoimmune encephalomyelitis (eae), an animal model of ms, through up-regulating t helper ... | 2012 | 22047987 |
| Genetic diversity of Schistosoma japonicum miracidia from individual rodent hosts. | Schistosoma japonicum is an important parasite in terms of clinical, veterinary and socio-economic impacts, and rodents, a long neglected reservoir for the parasite, have recently been found to act as reservoir hosts in some endemic areas of China. Any difference in the host's biological characteristics and/or associated living habitats among rodents may result in different environments for parasites, possibly resulting in a specific population structure of parasites within hosts. Therefore know ... | 2011 | 22051401 |
| vitamin e reduces hepatic fibrosis in mice with schistosoma japonicum infection. | to investigate whether vitamin e protects against hepatic fibrosis in mice with schistosoma japonicum infection, 24 pathogen-free kunming mice were selected and randomly divided into four groups: control (uninfected, untreated), model (infected, untreated), low-dose intervention (infected, vitamin e-treated, 30 mg/g bodyweight/day) and high-dose intervention (infected, vitamin e-treated, 60 mg/g bodyweight/day). mice were infected with schistosoma japonicum by inoculating abdominal skin with sna ... | 2012 | 22052067 |
| molecular cloning and characterization of a hsp70 gene from schistosoma japonicum. | schistosoma japonicum is the pathogen responsible for schistosomiasis japonica, one of the major infectious diseases targeted for prevention nationally in china. expression of heat shock proteins (hsps) following stress plays a very important biological role in many organisms including s. japonicum. among the hsp family, the 70-kda hsps are most responsible for intracellular chaperone and extracellular immunoregulatory functions. based on the published sequences in genbank/embl (af044412.1), ope ... | 2011 | 22076051 |
| hox genes from the parasitic flatworm schistosoma japonicum. | hox genes are characterized by a highly conserved peptide domain and contribute to antero-posterior axis patterning during embryogenesis. these genes have been widely studied in a variety of animal species due to their central role in evolutionary developmental biology. based on the published genome assembly and unpublished re-sequencing project data, we present the first genome-wide characterization and comparative genomic analysis of the hox gene family within schistosoma japonicum. eight hox ... | 2012 | 22100282 |
| an iminosugar n-pentafluorobenzyl-1-deoxynojirimycin as a novel potential immunosuppressant for the treatment of th2-related diseases. | increased levels of th2 cytokine interleukin-4 (il-4) have been reported to be involved in the pathogenesis of the parasite schistosoma japonicum (s. japonicum) infection or detected in the serum of the causative agent of acquired immunodeficiency syndrome (aids) patients. this correlates with a worsened outcome of aids. the inhibition of a th2 type response might aid in the treatment of these th2-related diseases. previously, we found that n-pentafluorobenzyl-1-deoxynojirimycin (5f-dnm), a new ... | 2012 | 22101135 |
| dynamics of th17 cells and their role in schistosoma japonicum infection in c57bl/6 mice. | the current knowledge of immunological responses to schistosomiasis, a major tropical helminthic disease, is insufficient, and a better understanding of these responses would support vaccine development or therapies to control granuloma-associated immunopathology. cd4(+) t cells play critical roles in both host immune responses against parasitic infection and immunopathology in schistosomiasis. the induction of t helper (th)1, th2 and t regulatory (treg) cells and their roles in schistosome infe ... | 2011 | 22102924 |
| Progress with schistosome transgenesis. | Genome sequences for Schistosoma japonicum and Schistosoma mansoni are now available. The schistosome genome encodes ~13,000 protein encoding genes for which the function of only a minority is understood. There is a valuable role for transgenesis in functional genomic investigations of these new schistosome gene sequences. In gain-of-function approaches, transgenesis can lead to integration of transgenes into the schistosome genome which can facilitate insertional mutagenesis screens. By contras ... | 2011 | 22124549 |
| molecular detection of schistosoma japonicum in infected snails and mouse faeces using a real-time pcr assay with fret hybridisation probes. | a real-time polymerase chain reaction (pcr) assay with fluorescence resonance energy transfer (fret) hybridisation probes combined with melting curve analysis was developed to detect schistosoma japonicum in experimentally infected snails and in faecal samples of infected mice. this procedure is based on melting curve analysis of a hybrid between an amplicon from the s. japonicum internal transcribed spacer region 2 sequence, which is a 192-bp s. japonicum-specific sequence, and fluorophore-labe ... | 2011 | 22124555 |
| schistosomal glomerulopathy and changes in the distribution of histological patterns of glomerular diseases in bahia, brazil. | distinct patterns of glomerular lesions, including membranoproliferative glomerulonephritis and focal segmental glomerulosclerosis, are associated with infection by schistosoma mansoni or schistosoma japonicum. evidence suggests that immune complex deposition is the main mechanism underlying the different forms of schistosomal glomerulonephritis and that immune complex deposition may be intensified by portal hypertension. the relationship between focal segmental glomerulosclerosis and schistosom ... | 2011 | 22124564 |
| what is the role of health education in the integrated strategy to control transmission of schistosoma japonicum in china? | in 2009, wang et al.'s field trial published in the new england journal of medicine, reported that a comprehensive strategy aiming to reduce the roles of humans and cattle as sources of schistosoma japonicum infection in snails was implemented and proved effective and promising in dramatically reducing the percentage of infected humans and snails, which had been extended to other endemic provinces in china afterwards. this implies that the integrated schistosomiasis-control strategies of interve ... | 2011 | 22127388 |
| evaluation of an igy-based immunomagnetic enzyme-linked immunosorbent assay system for detection of circulating schistosoma japonicum antigen in serum samples from patients in china. | we have developed a novel egg yolk antibody (igy)-coated magnetic beads antigen-capture immunoassay for detection of a circulating antigen of schistosoma japonicum in serum samples of patients in schistosomiasis-endemic areas of china. this igy-based immunomagnetic bead enzyme-linked immunosorbent assay (igy-imb-elisa) uses polyclonal igy-coated magnetic beads as a capture antibody, and a monoclonal igg as a detection antibody. the sensitivity of the magnetic immunoassay was 100% (40 of 40) in c ... | 2011 | 22144443 |
| recent advances in schistosoma genomics. | schistosome research has entered the genomic era with the publications reporting the s. mansoni and s. japonicum genomes. schistosome genomics is motivated by the need for new control tools. however, much can also be learned about the biology of schistosoma, which is a tractable experimental model. in this paper, we review the recent achievements in the field of schistosome research and discuss future perspectives on genomics and how it can be integrated in a usable format, on the genetic mappin ... | 2011 | 22145587 |
| schistosoma japonicum ova maintains epithelial barrier function during experimental colitis. | to evaluate the impacts of schistosoma japonicum (s. japonicum) ova on the tight junction barriers in a trinitrobenzenesulfonic acid (tnbs)-induced colitis model. | 2011 | 22147983 |
| The dynamic expression of allograft inflammatory factor-1 in hepatic tissues and splenic cells of BALB/c mice with Schistosoma japonicum infection. | Allograft inflammatory factor-1 (AIF-1) was originally cloned from a rat heart allograft under chronic rejection. Data from many studies suggested an important role of AIF-1 in several inflammatory processes. The aim of this study was to examine the dynamic expression of AIF-1 and its association with the pathogenesis of hepatic schistosomiasis in BALB/c mice infected with S. japonicum. The expression of AIF-1 and tumour necrosis factor-alpha (TNF-a) was determined by enzyme-linked immunosorbent ... | 2012 | 22150368 |
| [surveillance and forecast system of schistosomiasis in jiangsu province. iv. establishment of schistosoma japonicum cercaria-killing method by spraying niclosamide suspension on water surface]. | to investigate the effect of suspension concentrate of niclosamide (scn) on killing cercariae of schistosoma japonicum on water surface, optimization and impact on fish, so as to establish an emergency-treatment intervention for rapidly killing cercariae and eliminating water infectivity. | 2011 | 22164370 |
| [Toxicity of several drugs against Schistosoma japonicum adult worms in vitro]. | To observe the toxicity of auranofin, cisplatin, adriamycin, compounds 4N, H, B, O against Schistosoma japonicum adult worms in vitro and their inhibition on thioredoxin glutathione reductase (TGR). | 2011 | 22164376 |
| [Effects of signal transducers and activators of transcription 4 and 6 on development of worms and granuloma formation in mice infected with Schistosoma japonicum]. | To explore the effects of STAT4 and STAT6 on the development of worms and granuloma formation in mice infected with Schistosoma japonicum. | 2011 | 22164377 |
| [characterization and preliminary application of six monoclonal antibodies against recombinant signal protein 14-3-3 of schistosoma japonicum]. | to identify the properties of six monoclonal antibodies (mcabs) against recombinant signal protein 14-3-3 of schistosoma japonicum, and investigate their value in the diagnosis of schistosomiasis. | 2011 | 22164379 |
| [study on manufacturing materials of imitation snails]. | to make imitation snails by using substances with chemotaxis to schistosoma japonicum miracidia. | 2011 | 22164382 |
| [epidemiological survey of suspected natural foci of schistosomiasis in luxi city]. | three schistosomiasis patients pathologically diagnosed were detected in luxi city, yunnan province, a non-endemic area of schistosomiasis. all the cases were hospitalized for serious intestinal diseases, and schistosoma japonicum eggs were detected under their colonic mucosa. the 3 patients all denied they had contacted with infested water outside their resident place, and it was suggested that they might be infected in the local area. | 2011 | 22164393 |
| [mathematical modeling as an aid in understanding transmission and control of schistosoma japonicum in hilly and mountainous regions of china]. | over the past years there has been an increasing trend in the application of mathematical models in the studies and control of infectious disease. with development of computing technologies and increasing access to them, it becomes appealing for public health professionals to use mathematical models in their work. in this paper based on our previous experience working on environmental determinants and control of schistosomiasis in the hilly and mountainous regions of sichuan province, we introdu ... | 2011 | 22164479 |
| [killing effect of sodium abietate on adult worms of schistosoma japonicum in vitro]. | to observe the killing effect of sodium abietate on adult male and female worms of schistosoma japonicum in vitro. | 2011 | 22164488 |
| [evaluation of early diagnostic value of 6 antigens from schistosoma japonicum in mice]. | to find out the candidate antigen for immunoreagent, which could be used to diagnose schistosoma japonicum infection early in mice. | 2011 | 22164490 |
| [Screening interaction protein of Schistosoma japonicum gynecophoral canal protein by phage display]. | To search the interaction protein of Schistosoma japonicum gynecophoral canal protein (SjGCP). | 2011 | 22164491 |
| [Destructive effect of eggs of Schistosoma japonicum on spleens in C57BL/6 mice]. | To observe the destructive effect of eggs of Schistosomajaponicum on the splenic structure in C57BL/6 mice. | 2011 | 22164493 |
| [effects of paeoniflorin on expressions of ctgf, pdgf and tnf-alpha in mice with hepatic fibrosis due to schistosoma japonicum infection]. | to observe the effects of paeoniflorin on the expressions of ctgf and pdgf in liver tissue of fibrosis and the serum level of tnf-alpha in mice infected with schistosoma japonicum, and to explore the protective effect and its mechanisms of paeoniflorin on liver fibrosis. | 2011 | 22164494 |
| [bioinformatics analysis of nicotinamide phosphoribosyl transferase of schistosoma japonicum]. | to perform the bioinformatics analysis of nicotinamide phosphoribosyl transferase of schistosoma japonicum so as to provide the evidence for the development and application of it. | 2011 | 22164495 |
| [evaluation of kato-katz technique combined with stool hatching test in diagnosis of schistosomiasis japonica]. | to develop an aetiology detection method with higher sensitivity and accuracy for schistosomiasis japonica. | 2011 | 22164505 |
| [Detection of urine antibodies to Schistosoma japonicum by magnetic particle affinity immunoassay]. | To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum. | 2011 | 22164507 |
| [Surveillance and forecast system of schistosomiasis in Jiangsu Province V monitoring of infectivity of Schistosoma japonicum miracidia of Yangtze River]. | To establish an approach to monitor the Schistosoma japonicum miracidia in waterbody of the Yangtze River, and monitor the infectivity of water body, so as to provide the evidence for tracing and controlling infection source and interrupting the transmission of schistosomiasis, and Oncomelania snail control. | 2011 | 22164606 |
| [investigation on prevalent trends and analysis of control period among floating population in schistosomiasis endemic areas of guichi district]. | to understand the characteristics of the floating population infected with schistosoma japonicum, so as to provide the evidence for constituting and optimizing control strategy. | 2011 | 22164613 |
| [application of f-elisa for immunodiagnosis of schistosomiasis japonica]. | to optimize the experimental conditions of fast enzyme-linked immunosorbent assay (f-elisa) and evaluate its performance for immunodiagnosis of schistosomiasis japonica. | 2011 | 22164616 |
| [Antibody responses to leucine aminopeptidase in Schistosoma japonicum infection]. | To investigate the antibody response to leucine aminopeptidase in the different stages of Schistosoma japonicum infection. | 2011 | 22164617 |
| [preparation and characteristics of schistosoma japonicum signaling protein 14-3-3]. | to prepare soluble recombinant signal transduction protein 14-3-3 of schistosoma japonicum (rsj14-3-3) and investigate its immunologic features. | 2011 | 22164619 |
| [endemic situation of schistosomiasis in guangdong province from 2004 to 2009]. | to understand the endemic situation of schistosomiasis in guangdong province. | 2011 | 22164627 |
| [historical consideration of the widespread infection of the hepatitis c virus in japan and use of a fishbone diagram to investigate the cause]. | about 75% of japanese liver cancer is caused by hepatitis c. widespread infection of the virus resulted from inadequate medical knowledge, as well as the political, economic and administrative conditions of the time. we investigated the association between the widespread infection of the hepatitis c virus and the historical events. we used a fishbone diagram to investigate the cause of widespread infection of the hepatitis c virus and considered the issue from a historical standpoint. we found c ... | 2011 | 22164687 |
| [multi-dimensional evaluation system for schistosomiasis japonica transmission dynamic model]. | to evaluate the simulation effect of schistosoma japonicum cellular automata (sjca) model by using the multi-dimensional evaluation system. | 2011 | 22164843 |
| [diagnostic efficiency of indirect hemagglutination assay kit for antibody detection of schistosomiasis japonica]. | to evaluate the clinical diagnostic efficiency of indirect hemagglutination assay kit (iha kit) for antibody detection of schistosomiasis japonica. | 2011 | 22164845 |
| [Cloning, expression and identification of Toll like receptor interacting protein gene of Schistosoma japonicum]. | To clone and express Schistosoma japonicum Toll like receptor interacting protein (SjTollip) in prokaryotic expression system and analyze its stage-specific transcription and expression. | 2011 | 22164852 |
| [expression and characterization of thioredoxin glutathione reductase of schistosoma japonicum]. | to prepare the recombinant thioredoxin glutathione reductase of schistosoma japonicum (sjtgr) with biological activity. | 2011 | 22164854 |
| [Dynamic changes of early immune responses to attenuated Schistosoma japonicum cercariae induced in BALB/c mice]. | To study the early immune activation and its dynamic changes between the attenuated cercariae immunized mice and the normal infected mice. | 2011 | 22164856 |
| [effects of male worm extraction on ultrastructure of cultured vitelline cells from schistosoma japonicum]. | to study the effects of male worm extraction on the proliferation and metabolic activity of cultured vitelline cells from schistosoma japonicum. | 2011 | 22164857 |
| [Susceptibilities of Oncomelania hupensis snails to Schistosoma japonicum miracidia from different hosts]. | To understand the susceptibilities of Oncomelania hupensis snails to Schistosoma japonicum miracidia from different hosts. | 2011 | 22164864 |
| Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis. | Reversible phosphorylation of proteins is a critical mechanism involved in physiological function of organisms, including Clonorchis sinensis. In the present study, One cDNA clone encoding protein phosphatase 2A (CsPP2A) was isolated from a C. sinensis adult cDNA plasmid library. The open reading frame of the novel gene contains 924 bp and encoded a putative protein of 307 amino acids. A similarity analysis showed high homology with Schistosoma japonicum (76.3%) and Homo sapiens (84.4%), respect ... | 2011 | 22167368 |
| osteopontin expression is associated with hepatopathologic changes in schistosoma japonicum infected mice. | to investigate osteopontin expression and its association with hepatopathologic changes in balb/c mice infected with schistosoma japonicum. | 2011 | 22171141 |
| Serological diagnosis of clonorchiasis: using a recombinant propeptide of cathepsin L proteinase from Clonorchis sinensis as a candidate antigen. | Clonorchiasis is a common zoonosis in southern and northeastern parts of China, especially in Guangdong, Guangxi and Jilin province. Anti-Clonorchis sinensis antibody detection by enzyme-linked immunosorbent assay (ELISA) has been used for epidemiological surveys of clonorchiasis for its convenience and celerity, but it is still a meaningful work to screen ideal diagnostic antigen or antibody subtype for improvement of diagnostic sensitivity and specificity and for judgement of curative effect. ... | 2011 | 22173451 |
| Gastrointestinal neoplasia associated with bowel parasitosis: real or imaginary? | Several parasitic species are well known to have carcinogenic properties, namely; Schistosoma hematobium (squamous cell carcinoma of the bladder) and the liver flukes Opisthorchis and Chlonorchis (cholangiocarcinoma). A large number of parasites are known to colonize the gastrointestinal tract. We sought to review the evidence that implicates these parasites in gastrointestinal neoplasia. Schistosoma japonicum, which is endemic primarily in east Asia, has been shown in multiple studies to convey ... | 2011 | 22174720 |
| immunodiagnostic efficacy of detection of schistosoma japonicum human infections in china: a meta analysis. | to assess the diagnostic efficacy of the currently most widely used indirect hemagglutination assay (iha) and enzyme-linked immunosorbent assay (elisa) for detection of schistosoma japonicum human infections. | 2012 | 22182637 |
| a cleaved amplified polymorphic sequence (caps) method for the identification of geographical isolates of schistosoma japonicum in china. | 2011 | 22185948 | |
| histopathological changes of juvenile schistosoma japonicum harbored in mice treated orally with mefloquine at a smaller single dose. | the histopathological changes of 14-day-old schistosoma japonicum induced by a smaller single dose of mefloquine have been studied. twenty-three mice infected with 60-80 s. japonicum cercariae for 14 days were treated orally with mefloquine at a single dose of 200 mg/kg (free base), and groups of three mice were killed at various intervals posttreatment. the liver of each mouse was removed, fixed and processed routinely, and examined by light microscopy. eight hours posttreatment, 38.2% and 39.8 ... | 2011 | 22190126 |
| Effectiveness of synthetic trioxolane OZ78 against Schistosoma japonicum in mice and rabbits. | Antischistosomal activities of a synthetic peroxide OZ78 (an ozonide carboxylic acid) against Schistosoma japonicum have been studied in mice and rabbits. Among 132 mice used, 30 of them were infected with 80-100 S. japonicum cercariae for collection of juvenile and adult schistosomes applied in in vitro tests. The remaining 102 mice were infected with 40 schistosome cercariae used for experimental treatment. Other 13 rabbits infected each with 200 schistosome cercariae were treated orally with ... | 2011 | 22200956 |
| confocal laser scanning microscopic observation on adult schistosoma japonicum harbored in mice following treatment with single-dose mefloquine. | the aim of the present study is to assess the mefloquine-induced alteration of adult schistosoma japonicum using confocal laser scanning microscopy (clsm). eight out of ten mice infected with 60-80 s. japonicum cercariae for 35 days were treated orally with mefloquine at a single dose of 400 mg/kg. four groups of two mice were killed at 24 h and 3, 7, and 14 days post-treatment, and schistosomes were collected by perfusion from the liver and mesenteric veins, fixed in 70% alcohol, stained with a ... | 2011 | 22200961 |
| characterization of micrornas in taenia saginata of zoonotic significance by solexa deep sequencing and bioinformatics analysis. | the beef tapeworm taenia saginata infects human beings with symptoms ranging from nausea, abdominal discomfort to digestive disturbances and intestinal blockage. in the present study, microrna (mirna) expressing profile in adult t. saginata was analyzed using solexa deep sequencing and bioinformatics analysis. a total of 15.8 million reads was obtained by solexa sequencing, and 13.3 million clean reads (1.73 million unique sequences) was obtained after removing reads smaller than 18 nt. ten cons ... | 2011 | 22203522 |
| loop-mediated isothermal amplification (lamp): early detection of toxoplasma gondii infection in mice. | abstract: background: toxoplasmosis is a widespread zoonotic parasitic disease that occurs in both animals and humans. traditional molecular assays are often difficult to perform, especially for the early diagnosis of toxoplasma gondii infections. here, we established a novel loop-mediated isothermal amplification targeting the 529 bp repeat element (529 bp-lamp) to detect t. gondii dna in blood samples of experimental mice infected with tachyzoites of the rh strain. findings: the assay was pe ... | 2012 | 22214421 |
| bioinformatics analysis of the structure and linear b-cell epitopes of aquaporin-3 from schistosoma japonicum. | to analyze the structure of aquaporins-3(aqp-3) from schistosoma japonicum(sjaqp-3) using bioinformatical methods, and to provid of references for vaccine targets research. | 2012 | 22221751 |
| negative regulation of schistosoma japonicum egg-induced liver fibrosis by natural killer cells. | the role of natural killer (nk) cells in infection-induced liver fibrosis remains obscure. in this study, we elucidated the effect of nk cells on schistosoma japonicum (s. japonicum) egg-induced liver fibrosis. liver fibrosis was induced by infecting c57bl/6 mice with 18-20 cercariae of s. japonicum. anti-asgm1 antibody was used to deplete nk cells. toll-like receptor 3 ligand, polyinosinic-polycytidylic acid (poly i:c) was used to enhance the activation of nk cells. results showed that nk cells ... | 2012 | 22235358 |
| genome-wide identification and characterization of a panel of house-keeping genes in schistosoma japonicum. | quantitative real-time polymerase chain reaction (qpcr), as one of the most sensitive and precise gene expression analysis methods, is frequently used to validate data obtained in high-through-put assays. qpcr requires reference genes with stable transcription for accurate normalization. however, no systematic studies on such genes have been performed in the genus schistosoma japonicum. in this study, eight novel candidate genes selected from a microarray analysis and four commonly used referenc ... | 2012 | 22245333 |
| correlation between hammerhead ribozyme-mediated eggshell protein gene cleavage and reproduction inhibition of schistosoma japonicum. | schistosoma japonicum (s. japonicum) is an extremely harmful pathogen, which infects humans and causes severe public health problems. to date, no effective therapeutic drugs for this pathogen are available. in this study, we designed and constructed three hammerhead ribozymes targeting the eggshell protein gene of s. japonicum (sjesg). the cleavage activities of these three ribozymes were determined using cleavage experiments. the in vi ... | 2012 | 22246067 |
| [studies on spray of niclosamide ethanolamine salt. i. development and effect assessment of spray of niclosamide ethanolamine salt]. | to develop a spray of niclosamide ethanolamine salt for prevention of bovine from schistosoma japonicum infection, and explore its characteristics and effect. | 2011 | 22263502 |
| [study on expression of treg cells in attenuated schistosoma japonicum cercariae immunized balb/c mice]. | to study the expression differences of cd4+cd25+foxp3+treg cells between the attenuated cercariae immunized mice and the normal infected mice and discuss the immune protection mechanisms of the mice immunized with attenuated cercariae. | 2011 | 22263504 |
| [evaluation of schistosomiasis control effect of buffalo removal in anxiang county]. | to evaluate the effect of a comprehensive schistosomiasis control strategy based on buffalo removal in a lake and marshland region. | 2011 | 22263506 |
| [inhibitory effect of total flavonoids of malus hupehensis on hepatic fibrosis induced by schistosoma japonicum in mice]. | to investigate the effects of total flavonoids of malus hupehensis on hepatic fibrosis in mice induced by schistosoma japonicum. | 2011 | 22263507 |
| praziquantel derivatives exhibit activity against both juvenile and adult schistosoma japonicum. | a praziquantel analog 10-hydroxy praziquantel and eight praziquantel/peroxide conjugates were synthesized. the biological activity of these compounds was evaluated against juvenile and adult stages of schistosoma japonicum. unlike praziquantel, 10-hydroxy praziquantel exhibits activity against both juvenile and adult schistosoma japonicumin. all hybrid compounds displayed modest to significant worm killing activity. the present study has important significance for the development of hybrid antis ... | 2012 | 22264473 |
| superhydrophobic surface-based magnetic electrochemical immunoassay for detection of schistosoma japonicum antibodies. | in this paper, a magnetic electrochemical immunoassay that uses a superhydrophobic surface-based analytical platform (ssap) has been initially developed for detection of schistosoma japonicum (sj) antibodies (sjab). the ssap is fabricated by modifying the inner surfaces of plastic test tubes with superhydrophobic polycarbonate coatings that show a water contact angle up to 160° and a water rolling angle less than 5°. in a noncompetitive sandwich format, the sjab immunoassay with magnetic particl ... | 2012 | 22270051 |
| biochemical characterization and differential expression of a 16.5-kilodalton tegument-associated antigen from the liver fluke fasciola hepatica. | a cdna encoding a 16.5-kda protein termed fhtp16.5 was identified by immunoscreening of a cdna library from fasciola hepatica adult flukes using pooled sera from rabbits infected with f. hepatica for 4 weeks. quantitative reverse transcriptase pcr (qpcr) analysis revealed that fhtp16.5 is not expressed in unembryonated eggs. it is poorly expressed in miracidia and highly expressed at the juvenile and adult stages; however, significant differences were found between the expression levels of fhtp1 ... | 2012 | 22278327 |
| effects of a recombinant schistosomal-derived anti-inflammatory molecular (rsj16) on the lipopolysaccharide (lps)-induced activated raw264.7. | macrophages as a principal component of immune system play an important role in the initiation, modulation, and final activation of immune response against pathogens including schistosomes. classical (m1) or alternative (m2) activation states of macrophage have different functions during infections. previously, we report that the schistosomal-derived anti-inflammatory molecule coding gene (named sj16) was isolated and the recombinant sj16 (rsj16) was expressed in escherichia coli. rsj16 has been ... | 2012 | 22281546 |
| the genome of a blood fluke associated with human cancer. | the sequencing of the genome and transcriptome of schistosoma haematobium, a highly prevalent blood fluke and human parasite with a proven link to malignant bladder cancer, marks the 160(th) anniversary of its discovery as the first schistosome known to infect humans. comparative genomic analyses of s. haematobium and the more prevalent human-schistosomiasis pathogens (schistosoma mansoni and schistosoma japonicum) identified both shared and distinct genomic features. | 2012 | 22281765 |
| pseudotumoral form of neuroschistosomiasis: report of three cases in ganzi, china. | the authors report three rare cases of neuroschistosomiasis lacking extracranial involvement. no parasitic eggs were detected in the stool with the kato-katz thick smear methods. computed tomography of the brains showed hypodense signals, and magnetic resonance imaging showed isointense signals on t1-weighted images, hyperintense signals on t2-weighted images, and intensely enhancing nodules in the brain after intravenous administration of gadolinium. high-grade gliomas were suspected, and opera ... | 2012 | 22302862 |
| micrornas: potentially important regulators for schistosome development and therapeutic targets against schistosomiasis. | micrornas (mirnas) are small, endogenous non-coding rna molecules that regulate gene expression post-transcriptionally by targeting the 3' untranslated region (3' utr) of messenger rnas. since the discovery of the first mirna in caenorhabditis elegans, important regulatory roles for mirnas in many key biological processes including development, cell proliferation, cell differentiation and apoptosis of many organisms have been described. hundreds of mirnas have been identified in various multicel ... | 2012 | 22309492 |
| the combined treatment of praziquantel with osteopontin immunoneutralization reduces liver damage in schistosoma japonicum-infected mice. | the aim of this study was to evaluate the therapeutic effects of osteopontin neutralization treatment on schistosome-induced liver injury in balb/c mice. we randomly divided 100 balb/c mice into groups a, b, c, d and group e. mice in all groups except group a were abdominally infected with schistosomal cercariae to induce a schistosomal hepatopathological model. mice in group c, d and group e were respectively administered with praziquantel, praziquantel plus colchicine and praziquantel plus neu ... | 2012 | 22309838 |
| murine cd8(+)t cell cytotoxicity against schistosomula induced by inoculation of schistosomal 22.6/26gst coupled sepharose 4b beads. | schistosomasis is a world-wide parasitic disease. although chemotherapy is the main treatment method for schistosomasis currently, it cannot prevent schistosome reinfection. up to now no effective vaccine is available to prevent schistosomiasis. dendritic cells (dcs) are one of the key players in the cellular immune response and play an important role in antigen presentation as antigen-presenting cells. here we reported a novel large particulate antigen, in which sepharose 4b beads were coated w ... | 2012 | 22326902 |