Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the primary structure of an entamoeba histolytica beta-hexosaminidase a subunit. | an entamoeba histolytica gene (hex-a1) that encodes subunit a of the lysosomal enzyme beta-hexosaminidase has been cloned and sequenced. the inferred 59 kda hex-a1 protein has the same molecular weight and 32% amino acid residue identity with the human and mouse proteins and 28% residue identity with the dictyostelium protein. northern blot analysis identified a mrna of approximately 1.6 kb, which is in agreement with the expected size of a mrna encoding the 522 amino acid hex-a1 protein. southe ... | 1995 | 7581340 |
| identification of immunogenic epitopes of the 170-kda subunit adhesin of entamoeba histolytica in patients with invasive amebiasis. | entamoeba histolytica causes amebic dysentery (ad) and liver abscess (ala). little is known about protective immunity to amebiasis, and studies in this area have been complicated by the paucity of defined ameba antigens. we examined the proliferative responses of peripheral blood mononuclear cells (pbmc) from patients with ad and ala to a recombinant protein containing a portion of the 170 kda adhesin of e. histolytica (170cr), and to two synthetic peptides (1 and 2) derived from the 170 kda seq ... | 1995 | 7581341 |
| entamoeba histolytica and giardia lamblia infections: current diagnostic strategies. | entamoeba histolytica and giardia lamblia constitute, in a world context, the two commonest intestinal protozoan parasites to affect man. therefore accurate diagnosis is of paramount importance if resultant infections are to be adequately managed. demonstration of the cyst or trophozoite stage in a faecal sample(s) (several newer techniques are available) remains the lynch-pin of diagnostic strategies; however, excretion of cysts, especially, is intermittent and evidence of infection is not alwa ... | 1995 | 7582374 |
| [detection of pathogenic entamoeba histolytica dna in liver abscess aspirates with polymerase chain reaction]. | polymerase chain reaction (pcr) was used to diagnose amebic liver abscess by detecting pathogenic entamoeba histolytica dna in liver aspirates from patients with liver abscess. oligonucleotide primers specific for gene encoding of the 30,000 molecule of pathogenic entamoeba histolytica were used in the test. liver aspirates from 23 cases with amebic liver abscess as evidenced by typical clinical findings or very high titres of anti-e. histolytica antibodies with elisa were found to be positive w ... | 1995 | 7587609 |
| increase in mrna of multiple eh pgp genes encoding p-glycoprotein homologues in emetine-resistant entamoeba histolytica parasites. | with the goal of understanding possible mechanisms of drug resistance by the protozoan parasite entamoeba histolytica (eh), two novel eh p-glycoprotein (pgp) genes (eh pgp5 and eh pgp6) were sequenced, and the expression of four eh pgp genes determined in wild-type (wt) clone a and emetine-resistant (emr) clone c2 amebae. the eh pgp5 gene encodes a 1301-amino acid (aa) protein that is similar to those of eh pgp1 (64% aa identity), eh pgp2 (61%), eh pgp6 (39%) and homo sapiens mdr (multidrug-resi ... | 1995 | 7590312 |
| entamoeba histolytica stimulates the unstable transcription of c-fos and tumour necrosis factor-alpha mrna by protein kinase c signal transduction in macrophages. | macrophages play an important role in the control of and resistance to entamoeba histolytica (e. histolytica). however, e. histolytica infections are characterized by suppression of cell-mediated immunity. to elucidate the molecular mechanisms whereby amoebae modulate host defences, we investigated whether the parasite elicits the 'immediate early' gene c-fos and cytokine tumour necrosis factor-alpha (tnf)-alpha mrna and determined the signal transduction pathways involved in naive bone marrow-d ... | 1995 | 7590881 |
| contact-dependent transfer of the galactose-specific lectin of entamoeba histolytica to the lateral surface of enterocytes in culture. | in a study to investigate early interactions of entamoeba histolytica with epithelial cell monolayers, we found that a monoclonal antibody (mab), cd6, against an ameba surface antigen recognized the lateral surface of epithelial cells after coculture with trophozoites. display of the cd6 antigen on the epithelial cells necessitated contact with active trophozoites. it was found neither at 4 degrees c, nor with prefixed trophozoites, nor with trophozoite-conditioned media, nor when a filter preve ... | 1995 | 7591055 |
| myosin ii is involved in capping and uroid formation in the human pathogen entamoeba histolytica. | the redistribution and capping of surface receptors on the human pathogen entamoeba histolytica was observed in the presence of concanavalin a (cona). capping was correlated with plasma membrane folding towards the rear of the amoeba and with uroid formation. the uroid is thought to play a role in the escape of amoebae from the host immune response. to localize myosin ii during capping, amoebae were incubated in the presence of cona and then analyzed by microscopy. myosin ii was three times more ... | 1995 | 7591071 |
| amoebicidal in vitro activity shown by some metronidazole analogues: biological response-reduction potential correlation. | 1995 | 7597003 | |
| the extracellular neutral cysteine proteinase of entamoeba histolytica degrades anaphylatoxins c3a and c5a. | we have shown previously that the extracellular cysteine proteinase of entamoeba histolytica trophozoites activates the alternative pathway of complement by specifically cleaving c3. this unique mechanism of complement activation leads to passive lysis of nonpathogenic, but not of pathogenic strains. in an attempt to investigate the relationship between the cleavage of complement components c3 and c5 and the pathogenesis of amebiasis, we investigated the production of the anaphylatoxins c3a and ... | 1995 | 7602103 |
| nucleotide sequence of the srrna gene of entamoeba gingivalis: applications for construction of a species-specific dna probe and phylogenetic analysis. | the small subunit ribosomal rna (srrna) gene of entamoeba gingivalis was amplified by pcr and the product of 1.9-kbp sequence was cloned into a plasmid vector puc18. four clones were isolated and sequenced. the insert dnas were 1918- to 1921-bp long and a+t rich (65.5%). the four srrna sequences of e. gingivalis were found to be aligned with those of nine related protozoans while searching for e. gingivalis-specific sequences. a sequence of 28 oligonucleotides was chosen, chemically synthesized, ... | 1995 | 7603363 |
| direct evidence for secondary loss of mitochondria in entamoeba histolytica. | archezoan protists are though to represent lineages that diverged from other eukaryotes before acquisition of the mitochondrion and other organelles. the parasite entamoeba histolytica was originally included in this group. ribosomal rna based phylogenies, however, place e. histolytica on a comparatively recent branch of the eukaryotic tree, implying that its ancestors had these structures. in this study, direct evidence for secondary loss of mitochondrial function was obtained by isolating two ... | 1995 | 7604025 |
| [amebic appendicitis with subsequent perforated pancolitis]. | amoebic appendicitis is very rare, occurring in about 0.5 to 1% of acute appendicitis in tropical countries. the most severe complication is transmural amoebic colitis with perforation, described in 1.6 to 3.2% of cases. the mortality of such cases can be very high (up to 80%). we present a swiss patient with amoebic appendicitis followed by severe perforated colitis; a total colectomy was necessary and the patient survived. because of the good results of amoebicidal therapy and because of the s ... | 1995 | 7607020 |
| isolation and characterization of species-specific dna probes from taenia solium and taenia saginata and their use in an egg detection assay. | cysticercosis results from ingestion of the eggs of the tapeworm taenia solium. reduction of the incidence of human and swine cysticercosis requires identification and treatment of individuals who carry the adult tapeworm. t. solium and taenia saginata eggs cannot be differentiated on the basis of morphology; thus, in order to improve existing methods for the diagnosis of taeniasis, we have developed highly sensitive, species-specific dna probes which differentiate t. solium and t. saginata. rec ... | 1995 | 7615742 |
| characterization of glycogen and amino acid pool of entamoeba histolytica by 13c-nmr spectroscopy. | trophozoite extracts of axenic entamoeba histolytica were investigated by natural-abundance 13c-nmr spectroscopy. the extracts were found to contain a high level of glycogen (30 mm glucose equivalents), which had a compact structure as suggested by alpha (1-->6) branch points every 5-6 glucose residues. as other major metabolites, we identified putrescine (9.5 mm) and the following free amino acids: tyrosine and phenylalanine (1 mm), glycine, lysine and methionine (2 mm), isoleucine (5 mm), prol ... | 1995 | 7620458 |
| an unusual amebic localization on the right hip. | we report the history of a 43-year-old man with a histopathologically confirmed invasive amebic abscess in his right hip. ct scan of the liver was normal. the amebic indirect hemagglutination (iha) test was positive with a titer of 1/1,024. the patient developed acute renal failure and died within 48 h of admission with multiple organ failure due to sepsis. | 1995 | 7622262 |
| development of amebicidal cell-mediated immunity in gerbils (meriones unguiculatus) immunized with the galactose-inhibitable adherence lectin of entamoeba histolytica. | the galactose-inhibitable adherence lectin of entamoeba histolytica is a protective antigen in the gerbil model of amebic liver abscess. to further understand the mechanisms of vaccine efficacy, we studied the cell-mediated immune response to the lectin in gerbils. splenocytes harvested from immunized gerbils demonstrated in vitro proliferation and production of interleukin-2 and gamma-interferon in response to purified adherence lectin (p < 0.05 for each compared to control splenocytes). spleno ... | 1995 | 7623198 |
| identification of entamoeba histolytica intracellular phospholipase a and lysophospholipase l1 activities. | entamoeba histolytica phospholipase a and lysophospholipase activities from a vesicular subcellular fraction (p30) were analyzed. the products, obtained using specific substrates labeled with 14c or 3h, indicated the presence of phospholipase a1 and a2 as well as lysophospholipase l1 activities. the enzymes detected could participate in phospholipid metabolism and the alkaline phospholipase a2 may contribute to e. histolytica cytopathogenicity. | 1995 | 7624291 |
| how many stool examinations are necessary to detect pathogenic intestinal protozoa? | recent reports have suggested that examination of one stool specimen is sufficient to detect protozoa in symptomatic patients. to verify this hypothesis, we examined specimens submitted to the regional laboratory of a large, prepaid health plan that provides primary care to a membership representative of the general population in northern california. we examined the number and results of stool specimen examinations in the two-month period before detection of entamoeba histolytica in 125 infected ... | 1995 | 7625530 |
| pattern of infection of intestinal parasites in sagbama community of the niger delta, nigeria. | a parasitology survey was conducted in sagbama community of the niger delta to determine the pattern of infection of intestinal parasites of man. 280 faecal samples were collected from randomly selected individuals and examined microscopically for ova, larvae and cysts of intestinal parasites. the overall infection rate was 33.6%. the frequency of the parasites encountered was as follows: ascaris lumbricoides (18.2%). entamoeba histolytica (6.4%). hookworm (5.4%). trichuris trichiura (3.5%), str ... | 1995 | 7626531 |
| multidrug resistance and p-glycoproteins in parasitic protozoa. | drug resistance has emerged as a devasting impediment to the treatment and control of diseases of parasitic origin. the underlying mechanisms that contribute to this drug resistance in field isolates, however, are poorly understood. members of the p-glycoprotein gene (pgp) family have been identified, cloned, and sequenced in plasmodia, leishmania, and entamoeba, and variations in pgp copy number and/or expression have been implicated as a basis for drug resistance in each of these genera. the s ... | 1995 | 7629055 |
| health status of pediatric refugees in buffalo, ny. | to characterize the health status of recent pediatric refugees. | 1995 | 7633543 |
| [gastrointestinal and urinary parasitic infection in children at a regional hospital center in togo: some epidemiological aspects]. | to determine the impact of parasitic infection of the digestive and urinary tract in children living in a rural area of togo, a retrospective study was conducted in a pediatric department of kara, togo. results revealed that 35% of the 1610 children between the ages of 0 and 16 years had positive tests for parasites in stools or urine and that 117 had more than one parasite. trichomonas intestinalis, entamoeba histolytica, schistosoma mansoni and necator americanus accounted for 86.5% of the par ... | 1995 | 7637613 |
| identification of an entamoeba histolytica gene encoding a protein homologous to prokaryotic disulphide oxidoreductases. | 1995 | 7637700 | |
| a multitude of circular dnas exist in the nucleus of entamoeba histolytica. | 1995 | 7637704 | |
| dispar makes its debut. | 1995 | 7639254 | |
| purification and molecular cloning of a major antibacterial protein of the protozoan parasite entamoeba histolytica with lysozyme-like properties. | a protein with potent antibacterial activity was purified to apparent homogeneity from pathogenic entamoeba histolytica. it resembles lysozyme in that it is a basic protein which degrades cell walls of micrococcus luteus, displays optimal activity at acidic ph, and shows a preference for gram-positive bacteria. the protein has a molecular mass of approximately 23 kda upon sds/page and is localized inside the cytoplasmic granules of the amoebae. the primary structure was elucidated by protein ana ... | 1995 | 7649184 |
| amino acid consumption by the parasitic, amoeboid protists entamoeba histolytica and e. invadens. | amino acid consumption by entamoeba histolytica and e. invadens has been measured in order to assess the possible roles of amino acids as energy substrates. mixtures of amino acids enhanced the growth of the parasites in complex medium and their survival in simple medium. the consumption of several amino acids by the parasites suspended in simple media was greater when glucose was absent, suggesting that they may act as alternative energy sources. under these conditions, asparagine was consumed ... | 1995 | 7649448 |
| [the complement fixation reaction: a traditional, updated method for the detection of antibodies in the diagnosis of infections]. | complement-fixation (cf) is still an important basic serologic test for the diagnosis of infectious diseases. in several areas of microbiology (viral, bacterial, parasitic and fungal) it has served as a reference standard against which other methods have been compared. its partial displacement by other techniques, as advocated in some recent literature, is often unfounded and uncritical; displacement is mostly due not to problems inherent in the method, but rather to the lack of reagents of sati ... | 1995 | 7651069 |
| [prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile]. | during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ... | 1994 | 7654292 |
| metastasis of entamoeba histolytica compared to colon cancer: one more step in invasion. | the multistep invasion process of metastasis is a well-conserved mechanism of normal symbiosis and pathogenic parasitosis. to illustrate this statement, we have compared cellular and molecular aspects of metastasis from colon cancer and enteric amebiasis caused by entamoeba histolytica. both types of pathogenic cell, cancer cells and amebic trophozoites, follow the same metastatic route for the liver and other organs. both establish invasion microecosystems implicating similar kinds of host cell ... | 1994 | 7657511 |
| entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin-1 alpha. | infection with the protozoan parasite entamoeba histolytica results in a high mortality worldwide. to initiate infection, e. histolytica trophozoites in the bowel lumen penetrate the epithelium, and cause extensive lysis of host cells. the acute amebic lesions in animal models are characterized by infiltration with inflammatory cells, particularly neutrophils. the acute host response is likely important for determining whether the infection will spread systemically, but little is known regarding ... | 1995 | 7657801 |
| importance of e. histolytica antigen in serodiagnosis and follow up of patients with amoebic liver abscess. | this study is composed of a community based study and a hospital based study. the community based study was conducted on 200 apparently healthy individuals randomly chosen from rural areas. they were examined clinically, sonographically and parasitologically for e. histolytica cysts and by elisa for circulating amoebic antigen. they were negative for antigenaemia at 1/16 which was considered the diagnostic titre of amoebic hepatitis. the hospital based study was carried out on 27 patients suffer ... | 1995 | 7665944 |
| a comparative study of experimental caecal amoebiasis and the evaluation of amoebicides. | caecal amoebiasis models (mouse, hamster and rat) were compared for amoebic infection and chemotherapeutic evaluation using a pathogenic strain of entamoeba histolytica of human origin. caecal infection in the hamster was of the acute type, with a large number of amoebae in the caecal tissue, whereas the amoebae in rats were seen in the lumen, on the mucosal surface. amoebic lesions in the mouse caecum were of moderate severity. the mouse model responded to both metronidazole (tissue amoebicide) ... | 1995 | 7668916 |
| the diagnosis of old and new gastrointestinal parasites. | for the foreseeable future, light microscopy will continue to be the method of choice for diagnosing gastrointestinal parasites. however, in selected circumstances, the use of commercially available immunofluorescent kits will supersede the use of routine light microscopy for diagnosis of giardia lamblia and cryptosporidium parvum. these techniques may used to diagnose invasive amebic infections caused by e. histolytica in the future. pooling stool samples from the same or even different patient ... | 1995 | 7671576 |
| immunological similarity between the 170 kd amoebic adherence glycoprotein and human beta 2 integrins. | amoebiasis is an important cause of morbidity and mortality in developing countries. virulence of entamoeba histolytica depends on the trophozoite's being able to bind to colonic epithelium; binding is mediated by the 170 kd subunit of a 260 kd lectin. we found, by microcytofluorometry and western blotting studies, that this 170 kd subunit protein shares an epitope with human beta 2 integrins--a subfamily of cell-surface glycoproteins expressed exclusively on leucocytes and involved in many adhe ... | 1993 | 7678048 |
| structural analysis and demonstration of the 29 kda antigen of pathogenic entamoeba histolytica as the major accessible free thiol-containing surface protein. | the 29 kda protein of pathogenic entamoeba histolytica is a cysteine-rich surface antigen which we recently characterized by cdna sequencing and by using monoclonal antibodies which differentiated between pathogenic and non-pathogenic clinical isolates. to determine the structure and biochemical attributes of this protein, a repertoire of immunological techniques using monoclonal antibodies, and radiolabelling were employed. we demonstrated that the 29 kda protein forms a 60 kda dimer and a high ... | 1993 | 7682280 |
| neutralizing monoclonal antibody epitopes of the entamoeba histolytica galactose adhesin map to the cysteine-rich extracellular domain of the 170-kilodalton subunit. | entamoeba histolytica adheres to human colonic mucins and colonic epithelial cells via a galactose-binding adhesin. the adhesin is a heterodimeric glycoprotein composed of 170- and 35-kda subunits. fragments of the hgl1 gene encoding the 170-kda subunit were expressed as recombinant fusion proteins in escherichia coli and reacted with anti-adhesin monoclonal antibodies (mabs) or pooled human immune sera. the mabs tested recognize seven distinct epitopes on the 170-kda subunit and have distinct e ... | 1993 | 7682994 |
| use of monoclonal anti-light subunit antibodies to study the structure and function of the entamoeba histolytica gal/galnac adherence lectin. | adherence of entamoeba histolytica trophozoites to host cells is mediated by a galactose (gal) and n-acetylgalactosamine (galnac)-specific surface lectin. the lectin is a heterodimeric protein composed of heavy (170 kda) and light (35-31 kda) subunits linked by disulfide bonds. polyclonal and monoclonal antibodies (mab) raised against a light subunit-glutathione-s-transferase fusion protein were used to probe its structure and function. four light subunit-specific mab were produced which recogni ... | 1994 | 7696848 |
| [epidemiology and etiology of infectious diarrheas. the case of mexico]. | gastrointestinal infections represent a health problem. it is estimated that 1647 million cases of diarrhea and 3.2 million deaths due to this cause occur among children less than five years of age per year. those belonging to this age group have 15 times more risk of dying because of diarrhea. cases of liquid acute diarrhea with blood represent 80% of cases, diarrhea with blood represent 10%. most frequent causes of liquid diarrhea are enterotoxigenic escherichia coli and rotaviruses and most f ... | 1994 | 7701141 |
| control of an amoebiasis outbreak in the philippi area near cape town. | previous studies in durban have shown that serological investigations, in combination with iso-enzyme electrophoresis, are invaluable for monitoring the endemicity of pathogenic strains of entamoeba histolytica. we therefore proposed that antibody profiles could be used to detect epidemic situations. an outbreak of amoebiasis in the normally non-endemic philippi area near cape town provided an opportunity for testing this hypothesis. seven of 9 patients presenting at a district hospital with inv ... | 1994 | 7709300 |
| [comparison of intestinal parasite infestation indexes among hiv positive and negative populations]. | this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ... | 1994 | 7715428 |
| amoebapores, a family of membranolytic peptides from cytoplasmic granules of entamoeba histolytica: isolation, primary structure, and pore formation in bacterial cytoplasmic membranes. | three peptides with pore-forming activity were isolated from the cytoplasmic granules of pathogenic entamoeba histolytica by acidic extraction, gel filtration and reversed-phase high-performance liquid chromatography. partial amino acid sequence analysis of the three active peptides revealed that the most abundant of them was amoebapore and the other two were isoforms thereof. cloning and sequencing of genomic dna resolved the amino acid sequence of the two newly recognized peptides. the three p ... | 1994 | 7715451 |
| complement sensitivity of entamoeba histolytica and various nonpathogenic amoeba species. | culture forms of the potentially pathogenic entamoeba histolytica were compared to those of the nonpathogenic species of e. dispar, e. hartmanni, e. coli, endolimax nana, and e. moshkovskii regarding the sensitivity to lysis by human complement activated through the alternative pathway. e. dispar was found unique in its complement resistance; all other nonpathogenic isolates resembled e. histolytica in that they were complement sensitive. thus, a state of complement sensitivity is not a particul ... | 1994 | 7716404 |
| [a coprological study of intestinal infections in northern morocco (provinces of tangier, tetuán and larache)]. | an epidemiological study on intestinal parasites has been carried out in northern morocco (province of tangier, tetuan and larache). a comparative study has been made at a provincial, city and areas (urban and rural) level. also the spi (simple parasitism index) and the cpi (comparative parasitism index) distribution. | 1994 | 7716431 |
| [report on the first nationwide survey of the distribution of human parasites in china. 1. regional distribution of parasite species]. | a nationwide (taiwan province not included) survey of the distribution of human parasites in china during 1988-1992 was conducted under the auspices of the ministry of public health, with stratified masses randomly sampling. a total of 2,848 pilot sites in 726 counties with a population of 1,477,742 were surveyed, according to unified standard, unified diagnostic method and control quality. the overall infection rate of parasites was 62. 632%. among them, the infection rate was over 50% in 17 pr ... | 1994 | 7720194 |
| [effect of indirect fluorescent antibody test for the diagnosis of amoebiasis]. | indirect fluorescent antibody test (ifat) was performed by the method of [symbol: see text] (1971) with some slight modifications for the diagnosis of amoebiasis. the antigen used was prepared from 48-hour culture of entamoeba histolytica (strains g2, g3, and g5) in las diphasic medium and only those amoebae clinging the culture were collected. the serum samples or blood drops on filter paper were positive by the improved ifat in all of the 54 cases of amoebic liver abscess and the positive reac ... | 1994 | 7720200 |
| prevalence of intestinal parasites in saudi children: a community-based study. | a community-based study was undertaken to determine the prevalence and types of intestinal parasites in the pediatric population of al-baha region, saudi arabia. stool samples were randomly collected from 19,939 children of whom 4208 (21.1%) were found to harbour intestinal parasites. the most affected age group was 5-9 years and the sex distribution was almost equal in all age groups. specific prevalence rates were giardia lamblia 9 per cent, entamoeba histolytica 5 per cent, hymenolepis nana 2 ... | 1995 | 7723130 |
| the primary structure of an entamoeba histolytica enolase. | 1995 | 7723780 | |
| [underestimation of intestinal protozoa as a cause of diarrhea in family practice]. | 1995 | 7723885 | |
| entamoeba histolytica: increase of enterotoxicity and of 53- and 75-kda cysteine proteinases in a clone of higher virulence. | we compared the enterotoxicity and cysteine proteinases (cp) of the low-virulence entamoeba histolytica hm1 strain with the highly virulent 1659 clone, derived from hm1 by hamster liver passages. enterotoxicity of 50,000 freeze-thawed trophozoites was determined on 0.28-cm2 intestinal segments mounted in ussing chambers; cp activity of nonidet-p40 amebal lysates was assayed by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and carbobenzoxy-l-arginine-l-arginyl-p-nitroaniline, ... | 1995 | 7729472 |
| entamoeba histolytica: induction and isotype analysis of antibody producing cell responses in peyer's patches and spleen after local and systemic immunization in male and female mice. | the purpose of this work was to determine if anti-amebic antibody producing cell responses could be elicited in peyer's patches and spleen in mice locally or systemically immunized with glutaraldehyde-fixed trophozoites of entamoeba histolytica (gft). the animals were inoculated with either a single or four doses of gft via intragastric, rectal, and intraperitoneal routes. the anti-amebic antibody producing cell responses were analyzed by a spot forming cell assay (elispot). the kinetics of anti ... | 1995 | 7729489 |
| inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from entamoeba histolytica. | calcium release from internal stores of entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of fura-2. emptying of inositol(1,4,5)trisphosphate (ins(1,4,5)p3)-sensitive calcium pools in permeabilized e. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (ins(1,3,4,5)p4). similarly, prior depletion of ins(1,3,4,5)p4-sensitive stores did not have any influence on subsequent calcium release by ins(1,4,5)p3. the ... | 1995 | 7729520 |
| entamoeba histolytica autochthonous isolates from mentally retarded italian patients. | a total of 77 mentally retarded male inpatients residing in a psychiatric institution in northern italy were screened for the presence of stool parasites, entamoeba histolytica particularly. parasitological stool examination showed entamoeba spp. (e. histolytica and/or e. dispar) in 26 cases (33.7%). in vitro culture on robinson's medium was positive in 16 cases (61.1%); in 11 cases we could stabilize and clone the isolates and proceed to electrophoretic assays. in all cases, patterns of pathoge ... | 1995 | 7731923 |
| cyclic nucleotide changes induced in human leukocytes by a product of axenically grown entamoeba histolytica that inhibits human monocyte locomotion. | pulse exposure of human mononuclear phagocytes to the monocyte locomotion-inhibitory factor produced by entamoeba histolytica (i.e., the 369- to 765-da chromatographic fraction obtained from the supernatant fluid of axenically grown e. histolytica) led to a swift increase in the intracellular concentration of adenosine 3':5' cyclic monophosphate (camp). a weaker response was observed in human polymorphonuclear leukocytes, the locomotion of which, however, is not inhibited by this amebic factor. ... | 1995 | 7731924 |
| intestinal parasitic infections in the residents of an emigration camp in tijuana, mexico. | we examined stool specimens of the residents in the emigration camp in tijuana, mexico for helminth eggs or protozoan cysts with formalin-ethyl acetate concentration method in february and july 1992. out of 92 examined samples, number of positive was 49 (53.3%). while number of cumulative positive was 66 (71.7%). cysts of entamoeba coli (29.3%) giardia lamblia (9.8%), entamoeba histolytica (7.6%), and eggs of taenia spp. (6.5) were most frequently observed. filtered water supply and chemotherapy ... | 1995 | 7735788 |
| the antiamoebic effect of a crude drug formulation of herbal extracts against entamoeba histolytica in vitro and in vivo. | the antiamoebic effect of a crude drug formulation against entamoeba histolytica was studied. in the traditional system of medicine in india, the formulation has been prescribed for intestinal disorders. it comprises of five medicinal herbs, namely, boerhavia diffusa, berberis aristata, tinospora cordifolia, terminalia chebula and zingiber officinale. the dried and pulverized plants were extracted in ethanol together and individually. in vitro amoebicidal activity was studied to determine the mi ... | 1995 | 7739226 |
| primary structure of an entamoeba histolytica nicotinamide nucleotide transhydrogenase. | 1994 | 7739679 | |
| parasitic infestation in acute appendicitis. | 1995 | 7741603 | |
| clonal growth in petri dishes of entamoeba histolytica. | 1995 | 7747296 | |
| amoebiasis in ethiopia. | in a countrywide survey of amoebiasis, a total of 12,457 persons in 97 communities was stool examined by formol-ether concentration technique. the overall prevalences of entamoeba histolytica infections, as measured by rate of cyst-passers, in schoolchildren and non-school communities were 15.0% and 3.5%, respectively. slightly more females (18.4%) than males (14.2%) were infected among schoolchildren (p < 0.05) but the difference was not significant among non-school communities (p > 0.05). ther ... | 1995 | 7747328 |
| survey of the prevalence of intestinal parasites in children of primary school age. | a survey of the prevalence of intestinal parasites in primary school children was carried out in nimo in njikoka local government area of anambra state, nigeria. stool samples were taken from children belonging to eight (8) primary schools in the area. of the 1,536 stool samples examined using light microscopy after formalether centrifugation, 922 (60%) harboured one or more parasites. the prevalence of the parasites was as follows: ascaris lumbricoides (20.8%); trichuris trichiura, (15.3%); nec ... | 1994 | 7756189 |
| severe vaginal infection with entamoeba histolytica in a woman who recently returned from mexico: case report and review. | genital amebiasis is a rare complication of infection with entamoeba histolytica, even in areas where the pathogen is endemic. we describe a patient who apparently contracted intestinal amebiasis on a trip to mexico and who presented with ulcerative vulvovaginitis 2 months later. her condition rapidly progressed to severe necrotizing vulvovaginitis that required a radical vulvectomy. histopathologic examination of the surgical specimen revealed the presence of e. histolytica trophozoites. the pa ... | 1995 | 7756499 |
| [bacterial, viral and parasitic etiologies of acute infantile diarrhea in a rural senegal]. | we have studied the incidence of parasites, viruses and bacteria associated with acute infantile diarrhea in rural senegal. among parasitoses, cryptosporidium parvum (7.7%) is the most frequent, followed by entamoeba histolytica (5.1%). the first bacteriological etiologic agent is escherichia coli (20.9%). rotavirus are also frequently isolated (14.7%). prevalence of pathogens is higher: among children over 6 months and under two years old, among children either with mixed feeding or weaned, dur ... | 1993 | 7758380 |
| [amebic liver abscess: review of 9 cases]. | amoebiasis is an infection of the large intestine produced by the protozoan entamoeba histolytica. more than 10% of the world population is estimated to be infected. the italian pattern of this infection is similar to the eec trend: the prevalence is approximately 0.012% during the years '88 and '89. hepatic abscesses usually present with moderate fever, weight loss and abdominal pain. during the last ten years nine patients with amoebic liver abscesses were admitted to our hospital, six males a ... | 1994 | 7761679 |
| genetic differentiation of pathogenic and nonpathogenic strains of entamoeba histolytica by random amplified polymorphic dna polymerase chain reaction. | the random amplified polymorphic dna polymerase chain reaction (rapd-pcr) method was used to compare pathogenic and nonpathogenic strains of entamoeba histolytica. dna polymorphisms were detected among the different strains and dendrograms were constructed by phylip and paup analyses to study the relationship of the strains. both analyses resulted in identical results, which indicated that pathogenic strains of e. histolytica are closely related and clearly separated from the nonpathogenic strai ... | 1995 | 7770427 |
| intestinal parasitoses in the kandy area, sri lanka. | the prevalence of intestinal protozoa and geohelminths was assessed among two diverse populations in the kandy area: adults attending medical outpatients clinics at the teaching hospital peradeniya, and pre-school children in low-cost housing areas within the kandy municipality. in addition to a brief history and examination, a fresh stool sample was obtained and examined by direct smears in saline and iodine, and by formol-ether concentration. the children's stool samples were also examined for ... | 1994 | 7777909 |
| blastocystis hominis infection, a cause of human diarrhea. | blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ... | 1994 | 7777913 |
| polymerase chain reaction-solution hybridization enzyme-linked immunoassay (pcr-shela) for the differential diagnosis of pathogenic and non-pathogenic entamoeba histolytica. | 1995 | 7778145 | |
| [nationwide survey of the distribution of human parasites in china--infection with parasite species in human population]. | the infection rate of main species of parasites and their character by first nationwide survey of human parasites was made. the overall infection rate of human intestinal parasite and the infection rate of most species of parasites were higher in females than that in males. the infection rate according to the age group, the highest infection rate was found in the group aged 5-14 years. with regard to the relation between parasitic infections and occupations of the infected persons, the highest i ... | 1995 | 7788888 |
| [in vitro effect of metronidazole ester on trichomonas vaginalis and entamoeba histolytica]. | metronidazole ester, a new nitroimidazole drug, was synthesized by the department of physical chemistry, school of pharmacy, shanghai medical university. the trichomonadicidal and amoebicidal activity of metronidazole ester was evaluated in vitro by the cultivation of trichomonas vaginalis and entamoeba histolytica at different drug concentrations. the results indicated that the 100% lethal concentration for t. vaginalis and e. histolytica was 2.6 micrograms/ml and 40 micrograms/ml, respectively ... | 1995 | 7788891 |
| diagnosis of invasive amoebiasis: renaissance of the morphology era. | 1995 | 7797138 | |
| [recent progress in the diagnosis of intestinal amebiasis. contribution of molecular biology]. | only 10% of entamoeba histolytica strains have been reported to have a pathogenic capacity. theoretically and under certain conditions, confirming this estimation should limit the number and the cost of the treatments administered to the four hundred million asymptomatic carriers in the world. several new routine tests which allow more accurate and easier diagnosis of intestinal amoebiasis and in particular the differential diagnosis between the pathogenic and nonpathogenic strains are or soon w ... | 1994 | 7802347 |
| intestinal amebiasis: histopathologic features in experimentally infected mice. | intestinal amebiasis is still an important health problem in developing regions of the world. in order to advance our knowledge on the pathogenesis and to test the relevance of recently obtained in vitro observations, suitable in vivo experimental models of intestinal amebiasis are needed. in the past a variety of laboratory animals have been used, but the mouse, whose genetics and immunology is well known, has been seldomly used. therefore, entamoeba histolytica strain hm1:imss was directly ino ... | 1994 | 7803977 |
| antiprotozoal activities of benzimidazoles and correlations with beta-tubulin sequence. | benzimidazoles have been widely used since the 1960s as anthelmintic agents in veterinary and human medicine and as antifungal agents in agriculture. more recently, selected benzimidazole derivatives were shown to be active in vitro against two protozoan parasites, trichomonas vaginalis and giardia lamblia, and clinical studies with aids patients have suggested that microsporidia are susceptible as well. here, we first present in vitro susceptibility data for t. vaginalis and g. lamblia using an ... | 1994 | 7811023 |
| protection of gerbils from amebic liver abscess by immunization with recombinant entamoeba histolytica 29-kilodalton antigen. | the goal of our study was to obtain a highly conserved entamoeba histolytica recombinant antigen for study as a subunit amebiasis vaccine. we screened a uni-zap cdna library of e. histolytica (strain hm1:imss) with human immune sera and isolated a dominant 804-bp cdna clone. a 33-kda fusion protein expressed from the cdna clone was determined by monoclonal antibody binding, dna hybridization, and nucleotide sequence to be the complete e. histolytica 29-kda antigen. serum antibodies to the recomb ... | 1995 | 7822012 |
| immunization of rats with the 260-kilodalton entamoeba histolytica galactose-inhibitable lectin elicits an intestinal secretory immunoglobulin a response that has in vitro adherence-inhibitory activity. | the 260-kda galactose-inhibitable lectin of entamoeba histolytica mediates binding of amebic trophozoites to purified colonic mucins and intestinal epithelial cells. parenteral immunization of lewis rats with immuno-affinity-purified lectin with freund's adjuvant and then intra-peyer's patch inoculation of lectin with cholera toxin b subunit as adjuvant elicited a significant anti-lectin secretory immunoglobulin a response in the bowel lumen. purified intestinal secretory immunoglobulin a from t ... | 1995 | 7822040 |
| standardization and evaluation of elisa for the serodiagnosis of amoebic liver abscess. | an elisa test for the serological diagnosis of amoebic liver abscess (ala) was standardized and evaluated in sera from three groups of patients: (1) three patients with diagnosis confirmed by isolation of the parasite, (2) thirty seven patients with diagnosis established by clinical findings and ultrasound studies and (3) seven patients whose diagnosis were established by clinical findings and a positive double immunodifusion test. ninety one serum samples from healthy subjects and 22 from patie ... | 1994 | 7823821 |
| comparisons of ribosomal rna sequences from amitochondrial protozoa: implications for processing, mrna binding and paromomycin susceptibility. | the amitochondrial (a-mt) protozoa include four groups of organisms that are of interest as important human parasites and as probable descendents of the earliest branches of eukaryotic evolution. these organisms have not been directly compared in terms of structure and function of a specific molecule. we sequenced portions of their rrna-encoding genes coding for the internal transcribed spacers (its1 and 2) and adjoining small subunit (ss), 5.8s and large subunit (ls) rrnas. included are sites f ... | 1995 | 7828924 |
| cryptosporidiosis among medical patients with the acquired immunodeficiency syndrome in tikur anbessa teaching hospital, ethiopia. | fresh stool specimens, collected at random from 63 medical in-patients with acquired immunodeficiency syndrome (aids), were studied prospectively for cryptosporidium oocyst. the diagnosis of aids was made according to the clinical case definition of the bangui criteria. these patients presented with profuse watery diarrhoea, significant weight loss and other associated symptoms and signs of clinical manifestations of symptomatic human immunodeficiency virus (hiv) infection. using the modified ki ... | 1994 | 7835259 |
| enteropathogens associated with acute diarrhoeal diseases. | five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ... | 1994 | 7835992 |
| identification of a developmentally regulated transcript expressed during encystation of entamoeba invadens. | differentiation of trophozoites into cysts in entamoeba species has been described morphologically and to a lesser extent biochemically, but studies of stage specific gene expression have not been reported. at present entamoeba invadens is the only species that can be induced to differentiate in axenic culture and is a useful model system for the human parasite entamoeba histolytica. using this model system, we performed cdna-mrna hybridization experiments to compare the rna populations from tro ... | 1994 | 7838173 |
| cloning and sequencing a putative pyrophosphate-dependent phosphofructokinase gene from entamoeba histolytica. | pyrophosphate-dependent phosphofructokinase (ppi-pfk) gene from entamoeba histolytica was cloned from its genomic library and sequenced. the open reading frame has 1149 bp and codes for a protein of 41.5 kda. the deduced amino acid sequence of e. histolytica ppi-pfk has 25 to 28% identity to the ppi-pfks from propionibacterium freudenreichii, naegleria fowleri and potato. the amino acid residues known to contribute to the active site of ppi-pfk from p. freudenreichii are conserved. | 1995 | 7841199 |
| cysteine endopeptidases of entamoeba histolytica. | 1994 | 7845229 | |
| amebiasis from the "miraculous water of tlacote". | 1995 | 7845445 | |
| mexico. a stubborn amoeba takes center stage. | 1995 | 7846526 | |
| purification and immunologic characterization of a 30-kda cysteine proteinase of entamoeba histolytica. | a 30-kda cysteine proteinase was purified from extracts of axenically grown trophozoites of entamoeba histolytica strain hm1:imss. the purification procedure involved two consecutive chromatographic steps. sequence analysis revealed high similarity with histolysin and with other 27-kda cysteine proteinase. western-blot analysis using f(ab')2 fragments of a polyclonal antibody raised against the purified enzyme revealed that when the amebic extract was prepared in the absence of proteinase inhibi ... | 1994 | 7855127 |
| encysted ascites caused by entamoeba histolytica. | 1994 | 7860489 | |
| serodiagnostic evaluation of amoebic hepatitis. | 1994 | 7860573 | |
| effect of histamine and glucose on encystation of entamoeba histolytica in vitro. | isolates of entamoeba histolytica grown and maintained in modified boeck and drbohlav's medium were used to examine the effect of histamine and glucose on encystation process in vitro. this revealed that 60-74% of the active and motile trophozoites of e. histolytica encysted within 72 hours in the presence of histamine and glucose, in contrast to 10-20% encystment in controls (with out histamine and glucose); a highly significant difference (p < 0.001). these observations also suggest the possib ... | 1994 | 7863552 |
| [entamoeba histolytica (schaudinn 1903) and entamoeba dispar (e. brumpt 1925) are 2 different species]. | on the basis of epidemiologic and clinical data in humans, and of experimental studies in kittens, e. brumpt in 1925 showed that entamoeba histolytica producing quadrinucleate cysts was a complex of 2 species. he differentiated a pathogenic and invasive form, from a non pathogenic and non invasive one that he called entamoeba dispar. his explanation gained little support until recently, when sargeaunt reported a study of isoenzyme typing and allow then the classification of amoebae resembling e. ... | 1994 | 7866041 |
| entamoeba histolytica suppresses gamma interferon-induced macrophage class ii major histocompatibility complex ia molecule and i-a beta mrna expression by a prostaglandin e2-dependent mechanism. | the surface expression of class ii major histocompatibility complex immune-associated (ia) antigen is a principal accessory function of macrophages for antigen-specific t-cell activation and immunoregulation. to explore the mechanisms of impaired cell-mediated immunity in invasive amebiasis, we investigated the effects of entamoeba histolytica proteins on gamma interferon (ifn-gamma)-induced ia expression by murine bone marrow-derived macrophages. pretreatment of macrophages with secreted (condi ... | 1995 | 7868232 |
| molecular cloning of a 30-kilodalton lysine-rich surface antigen from a nonpathogenic entamoeba histolytica strain and its expression in a pathogenic strain. | a monoclonal antibody (mab), 318-28, that specifically reacts with a 30-kda antigen present on membrane surfaces of all nonpathogenic (np) entamoeba histolytica strains tested and which did not react with pathogenic (p) strains was used for the isolation of the cdna coding for this antigen from an expression library of an np e. histolytica strain. the deduced amino acid composition was rich in lysine residues (14.5%), with some sequence similarity to a polyadenylate-binding protein. southern and ... | 1995 | 7868264 |
| nucleotide sequence organisation and analysis of the nuclear ribosomal dna circle of the protozoan parasite entamoeba histolytica. | we have sequenced the extrachromsomal ribosomal dna (rdna) circle of the human protozoan parasite entamoeba histolytica hm-1:imss and present here the complete sequence organisation of the 24.5-kb molecule. each circle contains two 5.9-kb rdna transcription units organised as inverted repeats. the regions downstream (3543 bp) and upstream (9216 bp) of the rdnas contain various families of short tandem repeats. some of the upstream repeats share extensive sequence homology with the downstream rep ... | 1994 | 7870125 |
| induction of the iron-containing superoxide dismutase in entamoeba histolytica by a superoxide anion-generating system or by iron chelation. | the regulation of superoxide dismutase (sod) expression was studied in 4 entamoeba histolytica isolates. in comparison to anaerobic conditions, cultivation of the amoebae in the presence of superoxide radical anions or a ferrous iron chelator revealed substantial increase of sod expression. under the different culture conditions, all sod activity could be exclusively attributed to an iron-containing type (fesod). northern blot analysis revealed that fesod expression was regulated on the transcri ... | 1994 | 7870132 |
| [necrotizing amebic colitis]. | we report a case of necrotizing amebic colitis in a 43 year-old patient, 10 years after a stay in senegal, successfully treated by right hemicolectomy. entamoeba histolytica were found in colon necrosis and amoebiasis serology was positive, leading to the diagnosis. the epidemiologic, physiopathologic, clinical and therapeutic features of this exceptional affection are described. a few cases have been previously reported in non endemic areas where pre-operative diagnosis is rarely made. in endem ... | 1994 | 7875425 |
| the serine-rich entamoeba histolytica protein is a phosphorylated membrane protein containing o-linked terminal n-acetylglucosamine residues. | previously, we described the isolation of a cdna clone and the gene encoding a protective antigen of the protozoan parasite entamoeba histolytica, the serine-rich entamoeba histolytica protein (srehp). the derived amino acid sequence of the srehp cdna clone was remarkable for a high serine content (52/233 amino acids), a putative signal sequence, multiple hydrophilic dodecapeptide and octapeptide tandem repeats, and a hydrophobic c-terminal putative membrane-spanning region. here, we show that s ... | 1995 | 7876162 |
| a recombinant cysteine-rich section of the entamoeba histolytica galactose-inhibitable lectin is efficacious as a subunit vaccine in the gerbil model of amebic liver abscess. | the 170-kda subunit of the galactose-inhibitable adherence lectin of entamoeba histolytica mediates attachment to colonic mucins and host cells. the dna fragment encoding the 170-kda subunit was produced by polymerase chain reaction (pcr) and divided into four sections by restriction endonucleases. the third section (designated lc3, base pairs 2273-3397) encodes a cysteine-rich fusion protein that was recognized by adherence-inhibitory anti-lectin monoclonal antibodies and serum antibodies from ... | 1995 | 7876611 |
| [underestimation of intestinal protozoa as a cause of diarrhea in family practice]. | to assess the frequency of intestinal protozoa in stool samples of patients with diarrhoea in general practice. | 1995 | 7877701 |
| oral immunization with the dodecapeptide repeat of the serine-rich entamoeba histolytica protein (srehp) fused to the cholera toxin b subunit induces a mucosal and systemic anti-srehp antibody response. | the intestinal protozoan parasite entamoeba histolytica causes amebic dysentery, a major cause of morbidity worldwide. the induction of a mucosal antibody response capable of blocking amebic adhesion to intestinal cells could represent an approach to preventing e. histolytica infection and disease. here we describe the expression of a chimeric protein containing an immunogenic dodecapeptide derived from the serine-rich e. histolytica protein (srehp), fused to the cholera toxin b subunit (ctxb). ... | 1995 | 7890393 |