Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolation of antifungal compound from paeonia suffruticosa and its antifungal mechanism. | to isolate antifungal compound from paeonia suffruticosa, and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1,2,3,4,6-penta-o-galloyl-beta-d-glucose (pgg). | 2015 | 24577809 |
| purification of g1 daughter cells from different saccharomycetes species through an optimized centrifugal elutriation procedure. | centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. it can, for instance, isolate g1 daughter cells from a saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. the present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast spe ... | 2014 | 24604765 |
| metabolic engineering of candida glabrata for diacetyl production. | in this study, candida glabrata, an efficient pyruvate-producing strain, was metabolically engineered for the production of the food ingredient diacetyl. a diacetyl biosynthetic pathway was reconstructed based on genetic modifications and medium optimization. the former included (i) channeling carbon flux into the diacetyl biosynthetic pathway by amplification of acetolactate synthase, (ii) elimination of the branched pathway of α-acetolactate by deleting the ilv5 gene, and (iii) restriction of ... | 2014 | 24614328 |
| management of recurrent postoperative fungal endophthalmitis. | to report the management of recurrent postoperative fungal endophthalmitis (pofe) after failed pars plana vitrectomy (ppv) and antifungal therapy. | 2014 | 24618484 |
| promising results of cranberry in the prevention of oral candida biofilms. | in the context of dental caries prevention by natural foodstuff sources, antifungal and antibiofilm activities of dry commercial extracts of cranberry fruit (vaccinium macrocarpon aiton) and two other red fruits (vaccinium myrtillus l. and malpighia punicifolia l.) were assessed on candida albicans and candida glabrata yeasts. when added to the culture medium, the cranberry extract displayed a significant anti-adhesion activity against candida spp. when used at low concentrations. in addition, t ... | 2014 | 24623607 |
| the development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis. | accurate, rapid and economical fungal species identification has been a major aim in mycology. in this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (hrma) of internal transcribed regions its1 and its2 in ribosomal dna (rdna) for a rapid, simple and inexpensive differentiation of eight clinically relevant candida species (candida albicans, candida glabrata, candida parapsilosis, candida krusei, candida tropicalis, candida guilliermondii, candida dubli ... | 2014 | 24628973 |
| studies on the possible aetiological role of different candida species in pathogenesis of dentine caries by monitoring the calcium release from tooth particles. | an in vitro model has been developed for study of cariogenic potential of different candida species. slices were prepared from the root of extracted healthy teeth. these disks were covered with inert material, only the central hole, i.e. the root canal dentin surface remained uncovered. these preparates with free root dentin surfaces were incubated in sabouraud medium in the presence of six-six candida albicans, candida glabrata, candida tropicalis, candida krusei, candida inconspicua and candid ... | 2014 | 24631750 |
| dual antifungal properties of cationic antimicrobial peptides polybia-mpi: membrane integrity disruption and inhibition of biofilm formation. | with the increasing emergence of resistant fungi, the discovery and development of novel antifungal therapeutics were urgently needed. compared with conventional antibiotics, the limited propensity of amps to induce resistance in pathogens has attracted great interest. in the present study, the antifungal activity and its mechanism-of-action of polybia-mpi, a cationic peptide from the venom of social wasp polybia paulista was investigated. we demonstrated that polybia-mpi could potently inhibit ... | 2014 | 24642357 |
| effects of fluconazole on candida glabrata biofilms and its relationship with abc transporter gene expression. | candida glabrata has emerged as the second most prevalent fungal pathogen and its ability to form biofilms has been considered one of the most important virulence factors, since biofilms present a high tolerance to antifungal agents used in fungal infection treatment. the mechanisms of biofilm tolerance to antifungal agents remain poorly understood. thus, the aim of this study was to evaluate the effects of fluconazole (flu) on the formation and control of c. glabrata biofilms and its relation w ... | 2014 | 24645630 |
| determination of candida species nestled in denture fissures. | with the growing number of elderly individuals, candida is occasionally recognised as a fungal cause of aspiration pneumonia. in addition, there are numerous investigational reports on oral candidiasis. however, there are currently no reports on candida contamination of denture base materials. this study was conducted to investigate candida species in the oral cavity, denture parts and oral lesions of older/elderly subjects with oral candidiasis. the candida strains were isolated and the species ... | 2013 | 24648980 |
| in vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to staphylococcus aureus and yeast species. | we have studied the effect of inactivated microbial stimuli (candida albicans, candida glabrata, saccharomyces boulardii, and staphylococcus aureus) on the in vitro differentiation of lineage negative (lin(-)) hematopoietic progenitor mouse cells. purified lin(-) progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. all the stimuli assayed caused differentiation toward the myeloid lineage. s. boulardii and particularly c. gl ... | 2014 | 24650426 |
| the impact of new epidemiological cutoff values on candida glabrata resistance rates and concordance between testing methods. | interpretive criteria for candida susceptibility testing were recently revised with the establishment of species-specific epidemiological cutoff values (ecv). to assess the effect of modified cutoff values on candida glabrata resistance rates and agreement between testing methods, we tested the susceptibility of 598 clinical isolates to fluconazole, itraconazole, voriconazole, posaconazole, caspofungin, and amphotericin b using clsi m27-a3 and e-test methods. the caspofungin mics clustered above ... | 2014 | 24657169 |
| phenotypic analysis of a family of transcriptional regulators, the zinc cluster proteins, in the human fungal pathogen candida glabrata. | candida glabrata is the second most important human fungal pathogen. despite its formal name, c. glabrata is in fact more closely related to the nonpathogenic budding yeast saccharomyces cerevisiae. however, less is known about the biology of this pathogen. zinc cluster proteins form a large family of transcriptional regulators involved in the regulation of numerous processes such as the control of the metabolism of sugars, amino acids, fatty acids, as well as drug resistance. the c. glabrata ge ... | 2014 | 24657903 |
| candidemia surveillance in iowa: emergence of echinocandin resistance. | we performed prospective surveillance for candidemia at 14 iowa hospitals in 2011-2012. a total of 163 episodes were analyzed. candida albicans (n = 69 [42%]) and candida glabrata (n = 58 [36%]) were the most common species. antifungal resistance was uncommon; 9% of c. glabrata were fluconazole resistant, and 5% (3 isolates) were intermediate or resistant to 1 or more of the echinocandins. molecular analyses of the fks1 and fks2 hotspots of the c. glabrata revealed no mutations except in 2 of th ... | 2014 | 24666704 |
| species distribution and antifungal susceptibility profile of candida isolates from bloodstream infections in lima, peru. | yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the latin american region have been published. the aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in lima, peru and to determine their in vitro susceptibility to four antifungal drugs. we tested and identified 153 isolates collected between october 2009 and ... | 2014 | 24667770 |
| a novel echinocandin mig0310 with anticandida activity from newly isolated fusarium sp. strain ms-r1. | to isolate and identify an anticandida compound from newly isolated fusarium strain ms-r1 and characterization of its activity against standard and clinical strains of candida. | 2014 | 24674450 |
| synthesis, structure-activity relationships, and in vitro antibacterial and antifungal activity evaluations of novel pyrazole carboxylic and dicarboxylic acid derivatives. | a series of pyrazole-3-carboxylic acid and pyrazole-3,4-dicarboxylic acid derivatives were synthesized, the structures were confirmed by their nmr ((1)h and (13)c) and ft-ir spectra, and elemental analyses. the antibacterial and antifungal activities of the compounds against five bacterial and five fungal pathogens were screened using modified agar well diffusion assay. most of the molecules have inhibitory effects on both standard and clinical candida albicans strains. however, only the molecul ... | 2014 | 24681068 |
| antifungal susceptibility of candida isolates at one institution. | species distribution and antifungal susceptibility of candida isolates at one institution were evaluated. detection rates of fungi were examined for 5 years between 2007 and 2011. sensitivities of fungi to amphotericin b, flucytosine, fluconazole, micafungin, itraconazole, and voriconazole were evaluated in blood culture-positive patients. a total of 3,832 fungal isolates were detected, including candida albicans 66.5%, candida glabrata 20.3%, candida parapsilosis 6.2%, candida tropicalis 5.5%, ... | 2014 | 24682093 |
| in vitro evaluation of adherence of candida albicans, candida glabrata, and streptococcus mutans to an acrylic resin modified by experimental coatings. | this study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of candida albicans, candida glabrata, and streptococcus mutans to an acrylic resin. acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (hp) or sulfobetaine methacrylate (s), at concentrations of 25, 30, or 35%. half of the specimens were coated with saliva. the adhesi ... | 2014 | 24684564 |
| severe systemic candidiasis following immunomodulation therapy in in vitro fertilisation-embryo transfer (ivf-et). | a 41-year-old nulliparous woman, with a medical history of unexplained infertility and multiple in vitro fertilisation (ivf) cycles with immunosuppressive therapy, was admitted to our tertiary obstetrics unit with sepsis at 18 weeks of pregnancy with dichorionic diamniotic twins. candida glabrata was grown from her blood cultures, then subsequently from the liquor and placentae. she was treated with intravenous ambisome (amphotericin), but unfortunately, the infection resulted in the rupture of ... | 2014 | 24686802 |
| candida glabrata and pre-term premature rupture of membrane complicating in vitro pregnancy: case report and confirmation of mother to neonate transmission. | we report a case of candida glabrata infection in an in vitro fertilization-assisted pregnancy complicated by pre-term pre-labor rupture of the membranes. we found c. glabrata in gastric fluid, amniotic fluid and maternal vaginal swab. neonatal and maternal isolates showed indistinguishable molecular patterns analyzed by fingerprint dna multilocus. | 2014 | 24691825 |
| fatal case of candidemia due to candida glabrata. | 2014 | 24696185 | |
| enzymatic and hemolytic activity in different candida species. | candida species, in conditions of microbiota imbalance or decreased immune defenses, may be one of the main human fungal pathogens. virulence factors constitute the mechanisms used by the fungus to avoid host defenses. | 2015 | 24704439 |
| antioxidant and antifungal activities of smilax campestris griseb. (smilacaceae). | ethanol extract and fractions obtained from aerial parts of smilax campestris were examined in order to determine their phenolic composition, antioxidant capacity and antifungal activities. high-performance liquid chromatography coupled with dad analysis indicated that quercetin and rutin were the main phenolic compounds present in butanol fraction and ethanol extract, respectively. the antioxidant activity assessed by the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radical was significa ... | 2014 | 24707849 |
| isolation of cholesterol-dependent, multidrug-resistant candida glabrata strains from blood cultures of a candidemia patient in kuwait. | candida glabrata has emerged as an important human pathogen associated with systemic and mucosal infections. here, we describe isolation of two cholesterol-dependent candida glabrata strains from a candidemia patient which failed to grow on the media devoid of a cholesterol source. | 2014 | 24712485 |
| genome-wide survey of transcriptional initiation in the pathogenic fungus, candida glabrata. | dna sequencing of the 5'-flanking region of the transcriptome effectively identifies transcription initiation sites and also aids in identifying unknown genes. this study describes a comprehensive polling of transcription start sites and an analysis of full-length complementary dnas derived from the genome of the pathogenic fungus candida glabrata. a comparison of the sequence reads derived from a cdna library prepared from cells grown under different culture conditions against the reference gen ... | 2014 | 24725256 |
| enhancement of acetoin production in candida glabrata by in silico-aided metabolic engineering. | acetoin is a promising chemical compound that can potentially serve as a high value-added platform for a broad range of applications. many industrial biotechnological processes are moving towards the use of yeast as a platform. the multi-auxotrophic yeast, candida glabrata, can accumulate a large amount of pyruvate, but produces only trace amounts of acetoin. here, we attempted to engineer c. glabrata to redirect the carbon flux of pyruvate to increase acetoin production. | 2014 | 24725668 |
| multi-drug resistant oral candida species isolated from hiv-positive patients in south africa and cameroon. | candida species are a common cause of infection in immune-compromised hiv-positive individuals, who are usually treated with the antifungal drug, fluconazole, in public hospitals in africa. however, information about the prevalence of drug resistance to fluconazole and other antifungal agents on candida species is very limited. this study examined 128 candida isolates from south africa and 126 cameroonian candida isolates for determination of species prevalence and antifungal drug susceptibility ... | 2014 | 24726686 |
| histidine degradation via an aminotransferase increases the nutritional flexibility of candida glabrata. | the ability to acquire nutrients during infections is an important attribute in microbial pathogenesis. amino acids are a valuable source of nitrogen if they can be degraded by the infecting organism. in this work, we analyzed histidine utilization in the fungal pathogen of humans candida glabrata. hemiascomycete fungi, like c. glabrata or saccharomyces cerevisiae, possess no gene coding for a histidine ammonia-lyase, which catalyzes the first step of a major histidine degradation pathway in mos ... | 2014 | 24728193 |
| a seco-laurane sesquiterpene and related laurane derivatives from the red alga laurencia okamurai yamada. | a ring-cleaved sesquiterpene, named seco-laurokamurone, four laurane-type sesquiterpenes, laurepoxyene, 3β-hydroperoxyaplysin, 3α-hydroperoxy-3-epiaplysin, and 8,10-dibromoisoaplysin, one laurokamurane-type sesquiterpene, laurokamurene d, and one bisabolane-type sesquiterpene, (5s)-5-acetoxy-β-bisabolene, have been isolated from a re-collection of the red alga laurencia okamurai yamada, together with six other known sesquiterpenes. their structures, including relative configuration, were elucida ... | 2014 | 24731260 |
| in vitro antifungal susceptibility of candida glabrata to caspofungin and the presence of fks mutations correlate with treatment response in an immunocompromised murine model of invasive infection. | it has been argued that the in vitro activity of caspofungin (csp) is not a good predictor of the outcome of echinocandin treatment in vivo. we evaluated the in vitro activity of csp and the presence of fks mutations in the hot spot 1 (hs1) region of the fks1 and fks2 genes in 17 candida glabrata strains with a wide range of mics. the efficacy of csp against systemic infections from each of the 17 strains was evaluated in a murine model. no hs1 mutations were found in the eight strains showing m ... | 2014 | 24733474 |
| [place of vulvovaginal candidiasis in the lower genital tract infections and associated risk factors among women in benin]. | determine the place of vulvo-vaginal candidiasis (vvc) in the lower genital infections and seek risk factors among women in benin. | 2014 | 24746421 |
| evolution of bacterial consortia in spontaneously started rye sourdoughs during two months of daily propagation. | the evolution of bacterial consortia was studied in six semi-solid rye sourdoughs during long-term backslopping at different temperatures. each rye sourdough was started spontaneously in a laboratory (dough yield 200), propagated at either 20°c or 30°c, and renewed daily at an inoculation rate of 1∶10 for 56 days. the changes in bacterial diversity over time were followed by both dgge coupled with partial 16s rrna gene sequencing and pyrosequencing of bar-coded 16s rrna gene amplicons. four spec ... | 2014 | 24748058 |
| candida arteritis occurring in a liver transplant recipient. | we report the first case, to our knowledge, of candida arteritis in a liver transplant recipient. the patient presented with hemorrhagic shock requiring emergency arterial repair. as candida albicans, candida tropicalis, and candida glabrata were growing in the arterial tissue, the patient received antifungal therapy for 5 months, but died because of chronic graft dysfunction. no evidence of fungal infection was found in the tissue on postmortem examination. | 2014 | 24750364 |
| recurrent arthritis by candida glabrata, a diagnostic and therapeutic challenge. | infectious arthritis due to candida glabrata is very rare. a 40-year-old iranian man had developed a painful swelling on the left knee since a year ago. a surgery (meniscectomy) was performed on his knee. however, in follow-up visit after 2 months, the patient's condition was deteriorated. direct examination of synovial fluid with gram and hematoxylin-eosin stains were negative for any bacterial or fungal infection or crystal elements; however, inoculation into bactec™ mycosis ic/f and plus aero ... | 2014 | 24752453 |
| breakthrough invasive candida glabrata in patients on micafungin: a novel fks gene conversion correlated with sequential elevation of mic. | candida glabrata strains sequentially isolated from blood developed resistance to micafungin (mics from <0.015 to 4 μg/ml). a novel mutation identified in micafungin-resistant strains at bp 262 of fks2 (containing a deletion of f659 [f659del]) was inserted into the homologous region in fks1. | 2014 | 24789192 |
| identification of candida glabrata genes involved in ph modulation and modification of the phagosomal environment in macrophages. | candida glabrata currently ranks as the second most frequent cause of invasive candidiasis. our previous work has shown that c. glabrata is adapted to intracellular survival in macrophages and replicates within non-acidified late endosomal-stage phagosomes. in contrast, heat killed yeasts are found in acidified matured phagosomes. in the present study, we aimed at elucidating the processes leading to inhibition of phagosome acidification and maturation. we show that phagosomes containing viable ... | 2014 | 24789333 |
| efficacy and safety of antifungal additives in optisol-gs corneal storage medium. | optisol-gs, the most common corneal storage medium in the united states, contains antibacterial but no antifungal supplementation. most postkeratoplasty endophthalmitis and keratitis cases are now of a fungal origin. | 2014 | 24789459 |
| candida glabrata intra-abdominal candidiasis is characterized by persistence within the peritoneal cavity and abscesses. | the pathogenesis of candida glabrata infections is poorly understood. we studied the pathogenesis of intra-abdominal candidiasis (iac) in mice that were infected intraperitoneally with c. glabrata and sterile feces. c. glabrata bg2 (5 × 10(8) cfu) caused a 100% mortality rate. sublethal inocula of bg2 (1 × 10(8) or 1 × 10(7) cfu) caused peritonitis that progressed to abscesses. three clinical c. glabrata strains (5 × 10(8) cfu) caused 80 to 100% mortality rates, while a fourth (strain 346) cause ... | 2014 | 24799629 |
| yeast oropharyngeal colonization in human immunodeficiency virus-infected patients in central taiwan. | a prospective, cross-sectional study was conducted at a medical center in central taiwan to understand the prevalence, associated factors, and microbiologic features for oropharyngeal yeast colonization in human immunodeficiency virus-infected outpatients. oral yeast colonization was detected in 127 (45 %) patients, including 21 (16.5 %) colonized by more than one species. of the 154 isolates, candida albicans was the most common species (114, 74 %), followed by candida dubliniensis (10, 6.5 %), ... | 2014 | 24804977 |
| periocular and anterior orbital necrosis after upper eyelid gold weight loading: operation-related or self-inflicted? | a 44-year-old woman, who had undergone gold-weight implantation due to facial palsy and lagophthalmos, arrived at the ophthalmology ward with eyelid swelling and erythema, which rapidly deteriorated under intravenous antibiotics to a necrotic process involving the periocular tissues, the eye, and the anterior orbit. despite prompt removal of the gold weight, the patient's ocular and systemic condition continued to deteriorate, necessitating evisceration and debridement of necrotic tissue. cultur ... | 2014 | 24812491 |
| diagnostic usefulness of differential time to positivity for catheter-related candidemia. | a differential time to positivity (dtp) of ≥ 120 min is useful for diagnosing catheter-related bacteremia, but data on diagnosing catheter-related candidemia (crc) in this way are limited. we wished to evaluate the usefulness of the dtp for diagnosing crc. all adult patients who had the same candida species isolated from blood cultures drawn simultaneously from a central venous catheter (cvc) and a peripheral vein were included at a tertiary care hospital over an 18-month period. a total of 105 ... | 2014 | 24829236 |
| set of classical pcrs for detection of mutations in candida glabrata fks genes linked with echinocandin resistance. | clinical echinocandin resistance among candida glabrata strains is increasing, especially in the united states. antifungal susceptibility testing is considered mandatory to guide therapeutic decisions. however, these methodologies are not routinely performed in the hospital setting due to their complexity and the time needed to obtain reliable results. echinocandin failure in c. glabrata is linked exclusively to fks1p and fks2p amino acid substitutions, and detection of such substitutions would ... | 2014 | 24829248 |
| [study on candida infections in intensive care unit from 2008 to 2012]. | to investigate and analyze the candida infection situation at the intensive care unit (icu) in tianjin fourth central hospital from 2008 to 2012. | 2014 | 24831637 |
| polymerase chain reaction melting profiles of candida glabrata clinical isolates in solid organ transplant recipients in comparison to the other group of surgical patients. | the aim of the retrospective study were to estimate the prevalence of candida glabrata in liver and kidney transplant recipients compared to patients with short bowel syndrome receiving chronic total parenteral nutrition and relevance of the polymerase chain reaction melting profile (pcr mp) method for candida glabrata strains differentiation. c. glabrata clinical strains isolated from patients were identified by using standard mycological procedures. the analysis of genetic relatedness of the i ... | 2014 | 24836833 |
| milbemycin a4 oxime as a probe of azole transport in candida glabrata. | azole resistance in candida glabrata, a pathogenic yeast, has prompted studies of compounds that have therapeutic potential by reversing azole resistance. milbemycin a4 oxime blocked azole efflux and enhanced azole susceptibility fourfold in 28 clinical isolates of c. glabrata. specificity of the milbemycin a4 oxime effect depended on the drug transporter and the substrate being effluxed. the major effect of milbemycin a4 oxime was inhibition of azole and rhodamine 6g efflux by the atp-binding c ... | 2014 | 24838041 |
| in vitro antifungal activities of leaf extracts of lippia alba (verbenaceae) against clinically important yeast species. | there are few studies reporting the antifungal activities of lippia alba extracts. | 2014 | 24861304 |
| microbiocidal effects of various taurolidine containing catheter lock solutions. | we have recently shown that a catheter lock solution containing taurolidine dramatically decreases catheter-related bloodstream infections (crbsi) in patients on home parenteral nutrition (hpn) when compared to heparin. since several taurolidine formulations are commercially available, some of which also contain citrate or heparin, we were interested in the effect of these different locks on growth and biofilm formation of fungal, gram-negative and gram-positive pathogens that are known to imped ... | 2015 | 24861410 |
| candida glabrata: a deadly companion? | the yeast candida glabrata has become a major fungal opportunistic pathogen of humans since the 1980s. contrary to what its name suggests, it is much closer, phylogenetically, to the model yeast saccharomyces cerevisiae than to the most prevalent human fungal pathogen, candida albicans. its similarity to s. cerevisiae fortunately extends to their amenability to molecular genetics methods. c. glabrata is now described as part of the nakaseomyces clade, which includes two new pathogens and other e ... | 2014 | 24861573 |
| breakthrough invasive fungal disease in patients receiving posaconazole primary prophylaxis: a 4-year study. | posaconazole (psc) is currently recommended as primary prophylaxis in neutropenic patients with acute myeloid leukaemia (aml) and in allogenic haematopoietic stem cell transplantation (ahsct) recipients with graft-versus-host disease (gvhd). studies focusing on breakthrough invasive fungal disease (ifd) upon psc prophylaxis show disparate results. in order to evaluate the incidence of ifd in patients on psc prophylaxis and identify ifd risk factors, we carried out a retrospective study of all co ... | 2014 | 24861577 |
| antifungal resistance to fluconazole and echinocandins is not emerging in yeast isolates causing fungemia in a spanish tertiary care center. | accurate knowledge of fungemia epidemiology requires identification of strains to the molecular level. various studies have shown that the rate of resistance to fluconazole ranges from 2.5% to 9% in candida spp. isolated from blood samples. however, trends in antifungal resistance have received little attention and have been studied only using clsi m27-a3 methodology. we assessed the fungemia epidemiology in a large tertiary care institution in madrid, spain, by identifying isolates to the molec ... | 2014 | 24867979 |
| upc2a is required for high-level azole antifungal resistance in candida glabrata. | candida glabrata, the second most common cause of candida infections, is associated with high rates of mortality and often exhibits resistance to the azole class of antifungal agents. upc2 and ecm22 in saccharomyces cerevisiae and upc2 in candida albicans are the transcriptional regulators of erg11, the gene encoding the target of azoles in the ergosterol biosynthesis pathway. recently two homologs for these transcription factors, upc2a and upc2b, were identified in c. glabrata. one of these, up ... | 2014 | 24867980 |
| nosocomial candiduria in chronic liver disease patients at a hepatobilliary center. | nosocomial urinary tract infections (utis) are common in catheterized patients. fungal uti has become an important nosocomial problem over the past decade. the microbiology of candiduria is rapidly evolving and new trends are being reported. | 2014 | 24872653 |
| fks mutant candida glabrata: risk factors and outcomes in patients with candidemia. | echinocandins are recommended for candia glabrata candidemia. mutations in the fks1 and fks2 genes are associated with echinocandin resistance. few studies have assessed risk factors for fks mutant isolates and outcomes in patients receiving micafungin treatment. | 2014 | 24879785 |
| role of fks mutations in candida glabrata: mic values, echinocandin resistance, and multidrug resistance. | candida glabrata is the second leading cause of candidemia in u.s. hospitals. current guidelines suggest that an echinocandin be used as the primary therapy for the treatment of c. glabrata disease due to the high rate of resistance to fluconazole. recent case reports indicate that c. glabrata resistance to echinocandins may be increasing. we performed susceptibility testing on 1,380 isolates of c. glabrata collected between 2008 and 2013 from four u.s. cities, atlanta, baltimore, knoxville, and ... | 2014 | 24890592 |
| evaluation of multiplex pcr in first episodes of febrile neutropenia as a tool to improve early yeast diagnosis in leukemic/preleukemic patients. | in febrile neutropenic onco-hematological patients, delayed microbiological diagnosis leads to an increase in morbidity and mortality. identification of the microorganism changes antibiotic therapy in more than half of cases; however, in only 20-30 % of such cases pathogen isolation is achieved. this study evaluates the frequency of fungus infection and its etiology in onco-hematological patients with febrile neutropenia utilizing blood cultures and non-commercial multiplex polymerase chain reac ... | 2014 | 24897963 |
| time to positivity and detection of growth in anaerobic blood culture vials predict the presence of candida glabrata in candidemia: a two-center european cohort study. | this study shows the accuracy of exclusive or earlier growth in anaerobic vials to predict candida glabrata in a large series of candidemic patients from two european hospitals using the bactec 9240 system. alternatively, c. glabrata can be predicted by a time to positivity cutoff value, which should be determined for each setting. | 2014 | 24899027 |
| in vitro evaluation of the enzymatic activity profile of non-albicans candida species isolated from patients with oral candidiasis with or without diabetes. | to evaluate the expression of phospholipase (pl) and secreted aspartyl proteinase (sap) by candida glabrata and c tropicalis obtained from the denture biofilms of healthy participants (16 isolates), patients with oral candidiasis with diabetes (10 isolates), and patients with oral candidiasis without diabetes (25 isolates). | 2014 | 24908598 |
| fluconazole affects the alkali-metal-cation homeostasis and susceptibility to cationic toxic compounds of candida glabrata. | candida glabrata is a salt-tolerant and fluconazole (flc)-resistant yeast species. here, we analyse the contribution of plasma-membrane alkali-metal-cation exporters, a cation/proton antiporter and a cation atpase to cation homeostasis and the maintenance of membrane potential (δψ). using a series of single and double mutants lacking cnh1 and/or ena1 genes we show that the inability to export potassium and toxic alkali-metal cations leads to a slight hyperpolarization of the plasma membrane of c ... | 2014 | 24913686 |
| systemic candidiasis in extremely low birthweight (elbw) neonates despite the routine use of topical miconazole prophylaxis: trends, risk factors and outcomes over an 11-year period. | this study aims to determine the incidence, trends of systemic candidiasis and meningitis in extremely low birthweight (elbw) neonates (<1000 gms) despite the routine use of topical miconazole prophylaxis and to compare the risk factors, adverse outcomes and comorbidities with controls. | 2014 | 24919490 |
| candida albicans promotes invasion and colonisation of candida glabrata in a reconstituted human vaginal epithelium. | the principal aim of this study was to investigate the in vitro co-infection of a reconstituted human vaginal epithelium (rhve) by candida albicans and candida glabrata. | 2014 | 24924556 |
| pseudomonas aeruginosa inhibits the growth of pathogenic fungi: in vitro and in vivo studies. | the aim of the present study was to investigate the inhibitory effect of pseudomonas aeruginosa (pa) on pathogenic fungi, including candida albicans (ca), candida tropicalis (ct), candida glabrata (cg), candida parapsilosis (cp) and candida krusei (ck), in vitro and in vivo. in total, 24 pa strains were collected from clinical specimens and identified by gram staining, oxidase production and the api 20ne system. cross-streak, disk diffusion and co-culture methods were used to observe the inhibit ... | 2014 | 24926335 |
| a case of candida glabrata severe urinary sepsis successfully treated with micafungin. | candida glabrata is frequently resistant to fluconazole, and in advanced renal failure the safe use of this and other recommended drugs is limited. we report a case of a 56 years-old diabetic woman with renal failure and severe urinary sepsis from c. glabrata successfully treated with micafungin. | 2014 | 24936401 |
| evaluation of possibilities in identification and susceptibility testing for candida glabrata clinical isolates with the integral system yeast plus (isyp). | the aim of this study was to evaluate possibilities of correct identification and susceptibility testing of c. glabrata clinical isolates with integral system yeast plus (isyp). for species identification, as the reference method, api candida test and species-specific pcr reactions were used. the potential of antifungal susceptibility testing by the isyp test was compared with the sensititre yeast one. whilst the reference methods confirmed that the received population (n = 65 isolates) represen ... | 2014 | 24939684 |
| systematic phenotyping of a large-scale candida glabrata deletion collection reveals novel antifungal tolerance genes. | the opportunistic fungal pathogen candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. the inherent tolerance of c. glabrata to azole drugs makes this pathogen a serious clinical threat. to identify novel genes implicated in antifungal drug tolerance, we have constructed a large-scale c. glabrata deletion library consisting of 619 unique, individually bar-coded mutant strains, each lacking one specific gene, al ... | 2014 | 24945925 |
| yeast diversity associated to sediments and water from two colombian artificial lakes. | in colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. the main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in universidad ... | 2014 | 24948924 |
| silver colloidal nanoparticle stability: influence on candida biofilms formed on denture acrylic. | our aim in this study was to evaluate how the chemical stability of silver nanoparticles (sns) influences their efficacy against candida albicans and c. glabrata biofilms. several parameters of sn stability were tested, namely, temperature (50ºc, 70ºc, and 100ºc), ph (5.0 and 9.0), and time of contact (5 h and 24 h) with biofilms. the control was defined as sns without temperature treatment, ph 7, and 24 h of contact. these colloidal suspensions at 54 mg/l were used to treat mature candida biofi ... | 2014 | 24951722 |
| isavuconazole and nine comparator antifungal susceptibility profiles for common and uncommon candida species collected in 2012: application of new clsi clinical breakpoints and epidemiological cutoff values. | the in vitro activity of isavuconazole and nine antifungal comparator agents was assessed using reference broth microdilution methods against 1,421 common and uncommon species of candida from a 2012 global survey. isolates were identified using chromagar, biochemical methods and sequencing of its and/or 28s regions. candida spp. were classified as either susceptible or resistant and as wild type (wt) or non-wt using clsi clinical breakpoints or epidemiological cutoff values, respectively, for th ... | 2014 | 24952015 |
| development of multiplex loop-mediated isothermal amplification assays to detect medically important yeasts in dairy products. | rapid detection of yeast contamination is important in the food industry. we have developed loop-mediated isothermal amplification (lamp) assays to detect the emerging opportunistic pathogenic yeasts: candida albicans, candida glabrata, candida tropicalis, the candida parapsilosis group, trichosporon asahii, and trichosporon mucoides. these yeasts may cause deep-seated candidiasis or trichosporonosis. four lamp primer sets specific for candida were designed to target the internal transcribed spa ... | 2014 | 24965944 |
| [a case of micafungin-hyposensitive candida glabrata due to fks2 gene mutation]. | candida glabrata was continuously isolated in cultured urine samples from a subject with thrombotic thrombocytopenic purpura. yeast-like fungal phagocytosis found in gram staining led to agents being tested for antifungal susceptibility, revealing hyposensitivity to micafungin (mcfg) of mic < 2 mg/ml. mcfg administered for 10 days failed to cure c. glabrata infection. to clarify why hyposensitivity occurred, we analyzed the fks gene sequence using the pcr, finding a deficit of 3 bases coding phe ... | 2014 | 24979950 |
| yeasts vectored by migratory birds collected in the mediterranean island of ustica and description of phaffomyces usticensis f.a. sp. nov., a new species related to the cactus ecoclade. | nine yeast species belonging to genera candida, cryptococcus, phaffomyces, rhodotorula and wickerhamomyces, and one species of aureobasidium genus were isolated from the cloaca of migratory birds. candida glabrata and c. inconspicua were the species most frequently isolated and wickerhamomyces sylviae, which has recently been described as a new species isolated from bird cloaca, was again found. the majority of isolates showed the ability to grow up to 40 °c and/or at ph 3.0, two environmental c ... | 2014 | 24981278 |
| essential oil composition and antimicrobial activities of two closely related species, alpinia mutica roxb. and alpinia latilabris ridl., from peninsular malaysia. | the essential oils obtained by hydrodistillation of the unripe and ripe fruits of alpinia mutica roxb. and alpinia latilabris ridl. were analysed by capillary gc and gc-ms. the oils were principally monoterpenic in nature. the unripe and ripe fruit oils of a. mutica were characterized by camphor (21.0% and 15.8%), camphene (16.6% and 10.2%), β-pinene (8.6% and 13.5%), and trans,trans-farnesol (8.0% and 11.2%), respectively. the oils of the unripe and ripe fruits were moderately active against st ... | 2014 | 24987733 |
| a systematic analysis reveals an essential role for high-affinity iron uptake system, haemolysin and cfem domain-containing protein in iron homoeostasis and virulence in candida glabrata. | iron is an essential nutrient for all living organisms and human pathogens employ a battery of factors to scavenge iron from the high-affinity iron-binding host proteins. in the present study, we have elucidated, via a candidate gene approach, major iron acquisition and homoeostatic mechanisms operational in an opportunistic human fungal pathogen candida glabrata. phenotypic, biochemical and molecular analysis of a set of 13 c. glabrata strains, deleted for proteins potentially implicated in iro ... | 2014 | 24987864 |
| differential response of candida albicans and candida glabrata to oxidative and nitrosative stresses. | invasive candidiasis is associated with high mortality in immunocompromised and hospitalized patients. candida albicans is the main pathological agent followed by candida glabrata, candida krusei, candida parapsilosis, and candida tropicalis. these pathogens colonize different host tissues in humans as they are able to neutralize the reactive species generated from nitrogen and oxygen during the respiratory burst. among the enzymatic mechanisms that candida species have developed to protect agai ... | 2014 | 25002360 |
| [system metabolic engineering strategies for 2,3-butandione production by torulopsis glabrata]. | we regulated the carbon flux distribution of torulopsis glabrata cctcc m202019, an efficient pyruvate-producing microorganism, for improved 2, 3-butandione production. | 2014 | 25007652 |
| distribution of candida species isolated from blood cultures in hospitals in osaka, japan. | candida species are clinically important causes of bloodstream infections because their mortality is very high. given that some species of candida are azole-resistant, identifying the distributions of candida species could facilitate the formulation of an appropriate empirical antifungal therapy. it has been shown that the distribution varies depending on the continent, country, city, and hospital. in this paper, we describe the distributions of species in hospitals in northern osaka, japan. | 2014 | 25009091 |
| [evaluation of the vitek 2 system (ast-yso1 cards) for antifungal susceptibility testing against different candida species]. | the aim of this investigation was to evaluate the results of antifungal susceptibility for various candida species using the vitek 2 semi-automated system (ast-yso1 cards, biomérieux), and to compare them with those obtained by the clsi (clinical and laboratory standards institute) broth microdilution reference method (document m27-a3,2008). the essential agreement (ea) was > 90%, except for candida glabrata against voriconazole (vcz); and for candida krusei against fluconazole (fcz). the overal ... | 2014 | 25011594 |
| occurrence and characterization of candida nivariensis from a culture collection of candida glabrata clinical isolates in malaysia. | candida nivariensis and c. bracarensis have been recently identified as emerging yeast pathogens which are phenotypically indistinguishable from c. glabrata. however, there is little data on the prevalence and antifungal susceptibilities of these species. | 2014 | 25022264 |
| [case of candidemia with candida glabrata with confirmation of the acquisition of micafungin sensitivity due to new mutation of fks gene mutation]. | 2014 | 25033496 | |
| [fructose transporter in yeasts]. | study of hexoses transporter started with discovery of galactose permease in saccharomyces cerevisiae. glucose, fructose and mannose assimilation is assumed by numerous proteins encoded by different genes. to date over 20 hexoses transporters, belonging to sugar porter family and to major facilitator superfamily, were known. genome sequence analysis of candida glabrata, kluyveromyces lactis, yarrowia lipolytica, s. cerevisaie and debaryomyces hansenii reveled potential presence of 17-48 sugar po ... | 2014 | 25033548 |
| bpeaks: a bioinformatics tool to detect transcription factor binding sites from chipseq data in yeasts and other organisms with small genomes. | peak calling is a critical step in chipseq data analysis. choosing the correct algorithm as well as optimized parameters for a specific biological system is an essential task. in this article, we present an original peak-calling method (bpeaks) specifically designed to detect transcription factor (tf) binding sites in small eukaryotic genomes, such as in yeasts. as tf interactions with dna are strong and generate high binding signals, bpeaks uses simple parameters to compare the sequences (reads ... | 2014 | 25041923 |
| engineering the carbohydrate-binding site of epa1p from candida glabrata: generation of adhesin mutants with different carbohydrate specificity. | the n-terminal domain of the epa1p adhesin from candida glabrata (n-epa1p) is a calcium-dependent lectin, which confers the opportunistic yeast the ability to adhere to human epithelial cells. this lectin domain is able to interact with galactosides and, more precisely, with glycan molecules containing the galβ-1,3-galnac disaccharide, also known as the t-antigen. based on the crystallographic structure of the n-epa1p domain and the role of the variable loop cbl2 in glycan binding, saturation mu ... | 2014 | 25049238 |
| time-kill assay and etest evaluation for synergy with polymyxin b and fluconazole against candida glabrata. | fluconazole-resistant candida glabrata is an emerging pathogen that causes fungemia. polymyxin b, a last-resort antibiotic used to treat multidrug-resistant gram-negative bacterial infections, has been found to possess in vitro fungicidal activity and showed synergy with fluconazole against a single strain of c. glabrata. since both agents may be used simultaneously in intensive care unit (icu) patients, this study was performed to test for possible synergy of this combination against 35 c. glab ... | 2014 | 25049251 |
| how often do they have sex? a comparative analysis of the population structure of seven eukaryotic microbial pathogens. | the model of predominant clonal evolution (pce) proposed for micropathogens does not state that genetic exchange is totally absent, but rather, that it is too rare to break the prevalent pce pattern. however, the actual impact of this "residual" genetic exchange should be evaluated. multilocus sequence typing (mlst) is an excellent tool to explore the problem. here, we compared online available mlst datasets for seven eukaryotic microbial pathogens: trypanosoma cruzi, the fusarium solani complex ... | 2014 | 25054834 |
| synergistic antifungal effect of glabridin and fluconazole. | the incidence of invasive fungal infections is increasing in recent years. the present study mainly investigated glabridin (gla) alone and especially in combination with fluconazole (flc) against cryptococcus neoformans and candida species (candida albicans, candida tropicalis, candida krusei, candida parapsilosis and candida glabratas) by different methods. the minimal inhibitory concentration (mic) and the minimal fungicidal concentration (mfc) indicated that gla possessed a broad-spectrum ant ... | 2014 | 25058485 |
| fumaric acid production by torulopsis glabrata: engineering the urea cycle and the purine nucleotide cycle. | a multi-vitamin auxotrophic torulopsis glabrata strain, a pyruvate producer, was further engineered to produce fumaric acid. using the genome-scale metabolic model inx804 of t. glabrata, four fumaric acid biosynthetic pathways, involving the four cytosolic enzymes, argininosuccinate lyase (asl), adenylosuccinate lyase (adsl), fumarylacetoacetase (faa), and fumarase (fum1), were found. athough single overexpression of each of the four enzymes in the cytosol improved fumaric acid production, the h ... | 2015 | 25060134 |
| pivotal role for a tail subunit of the rna polymerase ii mediator complex cgmed2 in azole tolerance and adherence in candida glabrata. | antifungal therapy failure can be associated with increased resistance to the employed antifungal agents. candida glabrata, the second most common cause of invasive candidiasis, is intrinsically less susceptible to the azole class of antifungals and accounts for 15% of all candida bloodstream infections. here, we show that c. glabrata med2 (cgmed2), which codes for a tail subunit of the rna polymerase ii mediator complex, is required for resistance to azole antifungal drugs in c. glabrata. an in ... | 2014 | 25070095 |
| molecular epidemiology of candida albicans and candida glabrata strains isolated from intensive care unit patients in poland. | over the last decades, candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. the aim of this study was to evaluate the genetic diversity of 31 candida albicans and 17 candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in krakow between 2011- ... | 2014 | 25075783 |
| prognostic factors and historical trends in the epidemiology of candidemia in critically ill patients: an analysis of five multicenter studies sequentially conducted over a 9-year period. | to describe temporal trends in the epidemiology, clinical management and outcome of candidemia in intensive care unit (icu) patients. | 2014 | 25082359 |
| longer incubation times for yeast fungemia: importance for presumptive treatment. | isolation rates of candida glabrata at ≤2 days were 8.9% and 34.8% at >2 days; for cryptococcus neoformans, they were 0.9% and 8.6%, respectively (1741 fungemia analyzed). an incubation time >2 days supports candins as presumptive treatment for c. glabrata, keeping in mind the risk of cryptococcus fungemia. | 2014 | 25085022 |
| isolation, selection and evaluation of yeasts for use in fermentation of coffee beans by the wet process. | during wet processing of coffee, the ripe cherries are pulped, then fermented and dried. this study reports an experimental approach for target identification and selection of indigenous coffee yeasts and their potential use as starter cultures during the fermentation step of wet processing. a total of 144 yeast isolates originating from spontaneously fermenting coffee beans were identified by molecular approaches and screened for their capacity to grow under coffee-associated stress conditions. ... | 2014 | 25087206 |
| evaluation of blood culture media for the detection of fungi. | the aim of this study was to compare the utility of bactec™ mycosis-ic/f (mycosis), bactec™ plus aerobic/f (aerobic), and bactec™ plus anaerobic/f (anaerobic) media in the detection of fungi from simulated (obtained by the inoculation of tested media first with sterile sheep's blood and subsequently with one of 60 clinical yeast isolates) and clinical blood samples, taken during routine diagnostic examination in two hospitals. all tested strains grew on mycosis as well as aerobic bottles, and th ... | 2015 | 25098681 |
| nosocomial bloodstream candida infections in a tertiary-care hospital in south brazil: a 4-year survey. | the aims of this study were to evaluate the epidemiology of nosocomial candidemia in a tertiary hospital in south brazil and the in vitro antifungal susceptibility of isolates. blood strains from 108 patients were identified by pcr-based method. some 30.5 % of candidemia were caused by candida tropicalis, 28.7 % were due to candida albicans, 24.1 % with candida parapsilosis sensu stricto, 8.3 % with candida glabrata sensu lato, 1.8 % involved candida krusei and 6.6 % with other species. candidem ... | 2014 | 25103140 |
| trends in antifungal susceptibility of candida species--one year observation. | in the past years opportunistic fungal infections have seriously increased, mainly in immunocompromised patients. the aim of the study was to determine the prevalence of yeast-like fungi in invasive candidiasis and to estimate its susceptibility to chosen antifungal agents. one hundred and sixty strains of yeast-like fungi were cultured from various clinical material: samples from lower respiratory tract, blood, the peritoneal cavity and others. the susceptibility tests were established accordin ... | 2014 | 25115116 |
| prevalence of candida nivariensis and candida bracarensis in vulvovaginal candidiasis. | candida nivariensis and candida bracarensis were isolated from patients with vulvovaginal candidiasis (vvc). candida nivariensis and candida bracarensis were found in presumptive candida glabrata isolates, which were identified using the api candida system. we retrospectively re-examined vaginal presumptive candida glabrata isolates for candida nivariensis and candida bracarensis from january 1, 2003, through december 31, 2012, via detection of the its1 region and the 5.8s ribosomal rna gene. am ... | 2014 | 25118875 |
| candida glabrata endophthalmitis transmitted from graft to host after descemet stripping automated endothelial keratoplasty. | 2014 | 25124791 | |
| direct visualization of fungal infection in brains from patients with alzheimer's disease. | recently, we have reported the presence of fungal infections in patients with alzheimer's disease (ad). accordingly, fungal proteins and dna were found in brain samples, demonstrating the existence of infection in the central nervous system. in the present work, we raised antibodies to specific fungal species and performed immunohistochemistry to directly visualize fungal components inside neurons from ad patients. mice infected with candida glabrata were initially used to assess whether yeast c ... | 2015 | 25125470 |
| ten-year review of invasive candida infections in a tertiary care center in saudi arabia. | to review the epidemiology of invasive candida infections in a single center in saudi arabia over a subsequent 10-year period. | 2014 | 25129180 |
| activity of echinocandins and triazoles against a contemporary (2012) worldwide collection of yeast and moulds collected from invasive infections. | in this study, 1717 fungal clinical isolates causing invasive fungal infections were evaluated against nine antifungal agents using clinical and laboratory standards institute (clsi) reference broth microdilution methods. the isolates comprised 1487 candida spp., 109 aspergillus spp., 86 non-candida yeasts (including 52 isolates of cryptococcus neoformans) and 35 rare moulds obtained during 2012 from 72 hospitals worldwide. echinocandin resistance among candida spp. was low, and resistance rates ... | 2014 | 25129315 |
| environmental azole fungicide, prochloraz, can induce cross-resistance to medical triazoles in candida glabrata. | acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. the main goal of this study was to assess the development of cross-resistance between agricultural and clinical azoles by candida spp. an in vitro induction assay was performed, for a period of 90 days, with prochloraz (pcz) - an agricultural antifungal. afterward, the induced molecular resistance mechanisms were unveiled. mic value of pcz increased signi ... | 2014 | 25132632 |