Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| phylogenetic analysis of rhodococcus erythropolis based on the variation of ribosomal proteins as observed by matrix-assisted laser desorption ionization-mass spectrometry without using genome information. | rhodococcus erythropolis strains characterized as antibiotic producers can be classified into three groups according to their antibiotic spectrum and growth compatibility. due to their high genotypic similarity, the taxonomic relationship of these strains has not been elucidated. in this study, ribosomal protein profiling using matrix-assisted laser desorption ionization-mass spectrometry (maldi-ms) was employed to classify twenty-one strains of r. erythropolis (15 antibiotic producers and 6 non ... | 2009 | 19716527 |
| effects of inoculum size on cell-mediated and humoral immune responses of foals experimentally infected with rhodococcus equi: a pilot study. | the objective of this pilot study was to compare the cytokine profile as well as cell-mediated and antibody responses of foals infected with a low inoculum of virulent rhodococcus equi resulting in subclinical pneumonia to that of foals infected with a high inoculum resulting in severe clinical pneumonia. the mean (+/-sd) ratio of post-infection to pre-infection anti-r. equi igg(t) concentration was significantly (p=0.002) higher in foals infected with the high inoculum (195+/-145; range 62-328) ... | 2010 | 19720402 |
| identification of nitrile hydratase-producing rhodococcus ruber th and characterization of an amie-negative mutant. | microbial transformation of acrylonitrile to acrylamide by nitrile hydratase is of great interest to green chemistry. during the transformation, acrylic acid is generally accumulated as a by-product through biocatalysis of amidase. a novel strain with high nitrile hydratase activity was isolated from soil and identified as rhodococcus ruber th by morphology and 16s rrna gene analysis. an amidase-negative recombinant, r. ruber th3 was constructed. its nitrile hydratase activity was 25% higher tha ... | 2010 | 19720524 |
| viability of rhodococcus equi and parascaris equorum eggs exposed to high temperatures. | there is great concern about the potential pathogen contamination of horse manure compost spread in the same fields horses graze in. to ensure that pathogen destruction occurs, temperatures need to be sufficiently high during composting. here, we investigated the survival rate of two marker organisms, rhodococcus equi and parascaris equorum eggs, exposed to temperatures potentially encountered during horse manure composting. our results show that the time required to achieve a 1 log10 reduction ... | 2010 | 19727941 |
| isolation of identical nitrilase genes from multiple bacterial strains and real-time pcr detection of the genes from soils provides evidence of horizontal gene transfer. | bacterial enzymes capable of nitrile hydrolysis have significant industrial potential. microbacterium sp. aj115, rhodococcus erythropolis aj270 and aj300 were isolated from the same location in england and harbour identical nitrile hydratase/amidase gene clusters. strain aj270 has been well studied due to its nitrile hydratase and amidase activity. r. erythropolis itcbp was isolated from denmark and carries a very similar nitrile hydratase/amidase gene cluster. in this study, an identical nitril ... | 2009 | 19730817 |
| aerobic biotransformation of polybrominated diphenyl ethers (pbdes) by bacterial isolates. | polybrominated diphenyl ethers (pbdes) are flame retardants that have been used in consumer products and furniture for three decades. currently, very little is known about their fate in the environment and specifically about their susceptibility to aerobic biotransformation. here, we investigated the ability of the polychlorinated biphenyl (pcb) degrading bacteria rhodococcus jostii rha1 and burkholderia xenovorans lb400 to transform mono- through hexa-bdes at ppb levels. we also tested the pbde ... | 2009 | 19731666 |
| molecular characterization of the alkb gene in the thermophilic geobacillus sp. strain mh-1. | an extremely thermophilic alkane-degrading bacterium, strain mh-1, was isolated from the deep subterranean petroleum reservoir in shengli oil field, pr china. based on its physiological characteristics and analysis of its 16s rrna gene sequence, strain mh-1 was identified as geobacillus stearothermophilus. strain mh-1 was able to grow at temperatures ranging from 50 to 72 degrees c and effectively degraded hexadecane as the sole carbon source at 70 degrees c. strain mh-1 degraded alkanes with di ... | 2009 | 19733653 |
| isolation and identification of novel microcystin-degrading bacteria. | of 31 freshwater bacterial isolates screened using the biolog mt2 assay to determine their metabolism of the microcystin lr, 10 were positive. phylogenetic analysis (16s rrna) identified them as arthrobacter spp., brevibacterium sp., and rhodococcus sp. this is the first report of microcystin degraders that do not belong to the proteobacteria. | 2009 | 19734339 |
| regional limb perfusion with erythromycin for treatment of septic physitis and arthritis caused by rhodococcus equi. | 2009 | 19734562 | |
| standardization and validation of an immunoperoxidase assay for the detection of african horse sickness virus in formalin-fixed, paraffin-embedded tissues. | an immunoperoxidase assay for the detection of african horse sickness virus (ahsv) in formalin-fixed tissues is a valuable tool in the study of the pathogenesis of the disease, as well as a useful addition to existing diagnostic tests when only preserved tissues are available. an assay that uses hamblin antiserum in a basic avidin-biotin complex detection system was standardized and validated in accordance with the guidelines of the american association of veterinary laboratory diagnosticians su ... | 2009 | 19737761 |
| x-ray crystal structure of michaelis complex of aldoxime dehydratase. | aldoxime dehydratase (oxd) catalyzes the dehydration of aldoximes (r-ch=n-oh) to their corresponding nitrile (r-c triple bond n). oxd is a heme-containing enzyme that catalyzes the dehydration reaction as its physiological function. we have determined the first two structures of oxd: the substrate-free oxdre at 1.8 a resolution and the n-butyraldoxime- and propionaldoxime-bound oxdres at 1.8 and 1.6 a resolutions, respectively. unlike other heme enzymes, the organic substrate is directly bound t ... | 2009 | 19740758 |
| pharmacokinetics of erythromycin ethylsuccinate after intragastric administration to healthy foals. | plasma concentrations and pharmacokinetics of erythromycin and related compounds were determined after administration of erythromycin ethylsuccinate to six healthy male foals 3 to 5 months of age. hay was withheld from the foals overnight and erythromycin ethylsuccinate (25 mg/kg of body weight) was administered intragastrically. plasma erythromycin concentrations were determined at specific times after drug administration by high-performance liquid chromatography assay. maximum peak plasma conc ... | 2002 | 19750750 |
| degradation of hexane and other recalcitrant hydrocarbons by a novel isolate, rhodococcus sp. eh831. | hexane, a representative voc, is used as a solvent for extraction and as an ingredient in gasoline. the degradation of hexane by bacteria is relatively slow due to its low solubility. moreover, the biodegradation pathway of hexane under aerobic conditions remains to be investigated; therefore, a study relating to aerobic biodegradation mechanisms is required. consequently, in this study, an effective hexane degrader was isolated and the biodegradation pathway examined for the first time. in addi ... | 2010 | 19756804 |
| primary retroperitoneal abscesses due to rhodococcus equi in a patient with severe nephrotic syndrome: successful antibiotic treatment with linezolid and tigecycline. | we present a case of rhodococcus equi primary retroperitoneal abscesses without pulmonary involvement in an immunocompromised patient with severe nephrotic syndrome. no risk factors for exposure to r. equi were present. the infection was successfully treated with long-term combination antibiotic treatment including linezolid and tigecycline. | 2010 | 19758832 |
| disseminated rhodococcus equi infection in a kidney transplant patient without initial pulmonary involvement. | rhodococcus equi is increasingly recognized as an opportunistic pathogen in solid organ transplant recipients. primary pulmonary involvement is the most common finding. we report a case of a 42-year-old female kidney transplant recipient who developed multiple disseminated abscesses caused by r. equi while on adequate antimicrobial therapy. the patient presented with subcutaneous abscesses in the hip region and mamma and had 2 intracerebral abscesses. there were no clinical and radiologic signs ... | 2009 | 19762194 |
| [the introduction of the 9alpha-hydroxy group into androst-4-en-3,17-dione using a new actinobacterium strain]. | a 9alpha-hydrolase activity of a new actinobacterium strain identified as rhodococcus erythropolis based on the analysis of a 16s rrna gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (ad) was studied. in the presence of glucose in the medium, this strain completely transformed ad (4-20 g/l) into 9alpha-hydroxy-ad over 20-48 h. this culture was able to grow and perform ad 9alphahydroxylation at a concentration of dimethyl formamide up to 9%. crystalline 9alpha-hydroxy-ad was isol ... | 2009 | 19764613 |
| hydroxylation and further oxidation of delta9-tetrahydrocannabinol by alkane-degrading bacteria. | the microbial biotransformation of delta(9)-tetrahydrocannabinol was investigated using a collection of 206 alkane-degrading strains. fifteen percent of these strains, mainly gram-positive strains from the genera rhodococcus, mycobacterium, gordonia, and dietzia, yielded more-polar derivatives. eight derivatives were produced on a mg scale, isolated, and purified, and their chemical structures were elucidated with the use of liquid chromatography-mass spectrometry, (1)h-nuclear magnetic resonanc ... | 2009 | 19767471 |
| polyhydroxyalkanoates production by actinobacteria isolated from soil. | polyhydroxyalkanoates (phas) are biodegradable and renewable polymers produced by a wide range of bacterial groups. new microbial bioprospection approaches have become an important way to find new pha producers and new synthesized polymers. over the past years, bacteria belonging to actinomycetes group have become known as pha producers, such as nocardia and rhodococcus species, kineosphaera limosa liu et al. 2002, and, more recently, streptomyces species. in this paper, we disclose that there a ... | 2009 | 19767851 |
| aromatic hydrocarbon degradation genes from chronically polluted subantarctic marine sediments. | the goal of this study was to identify functional targets to detect polycyclic aromatic hydrocarbon (pah)-degrading bacterial populations in cold marine ecosystems. | 2009 | 19780966 |
| cloning, purification and characterization of two components of phenol hydroxylase from rhodococcus erythropolis upv-1. | phenol hydroxylase that catalyzes the conversion of phenol to catechol in rhodococcus erythropolis upv-1 was identified as a two-component flavin-dependent monooxygenase. the two proteins are encoded by the genes phea1 and phea2, located very closely in the genome. the sequenced phea1 gene was composed of 1,629 bp encoding a protein of 542 amino acids, whereas the phea2 gene consisted of 570 bp encoding a protein of 189 amino acids. the deduced amino acid sequences of both genes showed high homo ... | 2010 | 19787347 |
| extrapulmonary disorders associated with rhodococcus equi infection in foals: 150 cases (1987-2007). | to describe frequency, types, and clinical outcomes of extrapulmonary disorders (epds) in foals in which rhodococcus equi infection was diagnosed, and to identify factors determined at the time of admission that differentiated foals that developed epds from foals with r equi infection identified only in the lungs. | 2009 | 19793018 |
| rhodococcus equi virulence-associated protein a is required for diversion of phagosome biogenesis but not for cytotoxicity. | rhodococcus equi is a gram-positive facultative intracellular pathogen that can cause severe bronchopneumonia in foals and aids patients. virulence is plasmid regulated and is accompanied by phagosome maturation arrest and host cell necrosis. a replacement mutant in the gene for vapa (virulence-associated protein a), a major virulence factor of r. equi, was tested for its activities during macrophage infection. early in infection, phagosomes containing the vapa mutant did not fuse with lysosomes ... | 2009 | 19797071 |
| [bacterial biodiversity in the groundwater contaminated by oil]. | an oil-contaminated groundwater sample was collected at an abandoned petrochemical factory.bacterial genome dna was extracted for the 16s rdna gene amplification,and then a bacterial 16s rdna gene clone library was constructed. after the phylogenetic analysis of 16s rdna sequences, bacterial diversity and community structure of the groundwater were studied. the results showed the bacteria in the groundwater could be divided into 10 groups, which were as follows: gamma-proteobacteria (49.1%), alp ... | 2009 | 19799318 |
| lipase diversity in glacier soil based on analysis of metagenomic dna fragments and cell culture. | lipase diversity in glacier soil was assessed by culture independent metagenomic dna fragment screening and confirmed by cell culture experiments. a set of degenerate pcr primers specific for lipases of the hormone-sensitive lipase family was designed based on conserved motifs and used to directly pcr amplify metagenomic dna from glacier soil. these products were used to construct a lipase fragment clone library. among the 300 clones sequenced for the analysis, 201 clones encoding partial lipase ... | 2009 | 19809244 |
| immunohistological demonstration of rhodococcus equi in a trotter foal. | a 3-month-old female trotter foal was euthanized due to severe dyspnoea. pathomorphologically a chronic granulomatous to necrotizing pneumonia was found and rhodoccocus (r.) equi was isolated microbiologically. an immunohistological method using a murine monoclonal antibody against a 15-17 kda antigen of virulent r. equi was established in formalin-fixed and paraffin-embedded tissue sections using various antigen retrieval techniques to optimize the staining results. microwave treatment was most ... | 2009 | 19813451 |
| isolation and characterization of an atrazine-degrading rhodococcus sp. strain mb-p1 from contaminated soil. | the aim of this study is to isolate and characterize organisms capable of utilizing high concentration atrazine from the contaminated sites. | 2009 | 19818008 |
| biodesulfurization of dibenzothiophene by a newly isolated rhodococcus erythropolis strain. | a new dibenzothiophene (dbt) desulfurizing bacterium was isolated from oil-contaminated soils in iran. hplc analysis and pcr-based detection of the presence of the dbt desulfurization genes (dsza, dszb and dszc) indicate that this strain converts dbt to 2-hydroxybiphenyl (2-hbp) via the 4s pathway. the strain, identified as rhodococcus erythropolis sht87, can utilize dbt, dibenzothiophene sulfone, thiophene, 2-methylthiophene and dimethylsulfoxide as a sole sulfur source for growth at 30 degrees ... | 2010 | 19819129 |
| activation of foal neutrophils at different ages by cpg oligodeoxynucleotides and rhodococcus equi. | toll-like receptor 9 (tlr9) activation stimulates protective immune responses against intracellular pathogens by phagocytes, including neutrophils. this study examined tlr9-mediated neutrophil activation in neonatal foals. unmethylated cpgs, ligands for tlr9, were used to stimulate equine neutrophils, either purified or in contact with other peripheral blood leukocytes. rhodococcus equi was used as another stimulus in parallel. tlr9 mrna was constitutively expressed at a similar level in purifie ... | 2009 | 19819162 |
| structure and characterization of amidase from rhodococcus sp. n-771: insight into the molecular mechanism of substrate recognition. | in this study, we have structurally characterized the amidase of a nitrile-degrading bacterium, rhodococcus sp. n-771 (rhamidase). rhamidase belongs to amidase signature (as) family, a group of amidase families, and is responsible for the degradation of amides produced from nitriles by nitrile hydratase. recombinant rhamidase exists as a dimer of about 107 kda. rhamidase can hydrolyze acetamide, propionamide, acrylamide and benzamide with kcat/km values of 1.14+/-0.23 mm(-1)s(-1), 4.54+/-0.09 mm ... | 2010 | 19819352 |
| analysis of the dibenzothiophene metabolic pathway in a newly isolated rhodococcus spp. | out of 17 samples collected from diverse environments, 110 bacterial isolates of varied characteristics were screened for their dibenzothiophene-desulphurizing activity. a single isolate, eu-32, originating from a soil sample taken from the roots of a eucalyptus tree, displayed dibenzothiophene-desulphurizing activity. this isolate metabolized dibenzothiophene to 2-hydroxybiphenyl (2-hbp), as detected by hplc, and was also able to use other organic sulphur compounds as a sole sulphur source. bas ... | 2009 | 19824901 |
| rhodococcus equi infection in a surgical wound. | a 35-year-old male presented with abdominal pain one month after receiving a routine ventral hernia repair. over the course of two months, repeated wound cultures were ordered and eventually produced growth of rhodococcus equi. appropriate antibacterial therapy was initiated to resolve the infection. | 2009 | 19827407 |
| phylogenetic studies of nocardia species based on gyrb gene analyses. | phylogenetic analyses of 56 type species of nocardia were conducted using the partial nucleotide sequences of the gyrase b-encoding gene (gyrb). the interspecies similarities of the gyrb gene for the 56 type species were 82.4-99.9 %, which corresponded to 270-2 nt differences in the partial gene sequences of approximately 1200 nt. in comparison with phylogenetic relationships, gyrb gene sequence information was generally consistent with that of 16s rrna gene sequences with minor exceptions. howe ... | 2010 | 19833784 |
| degradation of a mixture of hydrocarbons, gasoline, and diesel oil additives by rhodococcus aetherivorans and rhodococcus wratislaviensis. | two strains, identified as rhodococcus wratislaviensis ifp 2016 and rhodococcus aetherivorans ifp 2017, were isolated from a microbial consortium that degraded 15 petroleum compounds or additives when provided in a mixture containing 16 compounds (benzene, toluene, ethylbenzene, m-xylene, p-xylene, o-xylene, octane, hexadecane, 2,2,4-trimethylpentane [isooctane], cyclohexane, cyclohexanol, naphthalene, methyl tert-butyl ether [mtbe], ethyl tert-butyl ether [etbe], tert-butyl alcohol [tba], and 2 ... | 2009 | 19837842 |
| cytochrome p450 125 (cyp125) catalyses c26-hydroxylation to initiate sterol side-chain degradation in rhodococcus jostii rha1. | the cyp125 gene of rhodococcus jostii rha1 was previously found to be highly upregulated during growth on cholesterol and the orthologue in mycobacterium tuberculosis (rv3545c) has been implicated in pathogenesis. here we show that cyp125 is essential for r. jostii rha1 to grow on 3-hydroxysterols such as cholesterol, but not on 3-oxo sterol derivatives, and that cyp125 performs an obligate first step in cholesterol degradation. the involvement of cyp125 in sterol side-chain degradation was conf ... | 2009 | 19843222 |
| mycobacterial cytochrome p450 125 (cyp125) catalyzes the terminal hydroxylation of c27 steroids. | cyp125 (rv3545c), a cytochrome p450, is encoded as part of the cholesterol degradation gene cluster conserved among members of the mycobacterium tuberculosis complex. this enzyme has been implicated in mycobacterial pathogenesis, and a homologue initiates cholesterol catabolism in the soil actinomycete rhodococcus jostii rha1. in mycobacterium bovis bcg, cyp125 was up-regulated 7.1-fold with growth on cholesterol. a cyp125 deletion mutant of bcg did not grow on cholesterol and accumulated 4-chol ... | 2009 | 19846551 |
| pegylation of bacterial cocaine esterase for protection against protease digestion and immunogenicity. | enhancing cocaine metabolism by administration of cocaine esterase (coce) has been considered as a promising treatment strategy for cocaine overdose and addiction, as coce is the most efficient native enzyme yet identified for metabolizing the naturally occurring cocaine. a major obstacle to the clinical application of coce, however, lies in its thermo-instability, rapid degradation by circulating proteases, and potential immunogenicity. pegylation, namely by modifying a protein or peptide compo ... | 2010 | 19857534 |
| genetic diversity of phthalic acid esters-degrading bacteria isolated from different geographical regions of china. | thirty-two strains of phthalic acid ester (paes)-degrading bacteria were isolated from thirteen geographically diverse sites by enrichment using mixtures of paes as the sole source of carbon and energy. sequence analyses of the 16s rrna gene indicated that these isolates were from six genera (arthrobacter, gordonia, rhodococcus, acinetobacter, pseudomonas, and delftia). to evaluate the genetic diversity among them, the molecular typing method rep-pcr with primers based on enterobacterial repetit ... | 2010 | 19866373 |
| in vivo 31p and 13c nmr investigations of rhodococcus rhodochrous metabolism and behaviour during biotransformation processes. | the strain rhodococcus rhodochrous obt18 was isolated from a water treatment plant used to decontaminate industrial effluents containing benzothiazole derivatives. aims of the work are to study the central metabolism of this strain and more specifically its behaviour during biodegradation of 2-aminobenzothiazole. | 2010 | 19878365 |
| aromatic hydroxylation of indan by o-xylene-degrading rhodococcus sp. strain dk17. | the metabolically versatile rhodococcus sp. strain dk17 utilizes indan as a growth substrate via the o-xylene pathway. metabolite and reverse transcription-pcr analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase. | 2010 | 19880642 |
| 7-ketocholesterol catabolism by rhodococcus jostii rha1. | oxysterols from steroid autooxidation have numerous harmful effects, but their biodegradation is poorly understood. microarrays were used to study mineralization of the most common oxysterol, 7-ketocholesterol (7kc), by rhodococcus jostii rha1. growth on 7kc versus growth on cholesterol resulted in 363 differentially expressed genes, including upregulation of two large gene clusters putatively encoding steroid catabolism. despite this difference, 7kc degradation required key genes involved in ch ... | 2010 | 19880645 |
| a positively charged amino acid at position 129 in nitrilase from rhodococcus rhodochrous atcc 33278 is an essential residue for the activity with meta-substituted benzonitriles. | a positively charged amino acid (arg, lys, or his) at position 129 in rhodococcus rhodochrous atcc 33278 nitrilase is essential for the activity of aromatic nitriles. the wild-type enzyme containing arg129 was active only for meta- and para-substituted benzonitriles with a methyl or amino group, but the r129k and r129h mutant enzymes were active only for meta-substituted benzonitriles. the lack of activity of the mutants for para-substituted benzonitriles may be attributable to steric hindrance ... | 2010 | 19903476 |
| identification of different alkane hydroxylase systems in rhodococcus ruber strain sp2b, an hexane-degrading actinomycete. | to investigate the alkane-hydroxylating system of isolate sp2b, closely related to rhodococcus ruber dsm 43338(t) and uncharacterized so far for its alkane degradation genes. | 2010 | 19912429 |
| methylcatechol 1,2-dioxygenase of rhodococcus opacus 6a is a new type of the catechol-cleaving enzyme. | the strains rhodococcus sp. 400, r. rhodochrous 172, and r. opacus 6a utilize 4-methylbenzoate as the only carbon and energy source. 4-methylcatechol is a key intermediate of biodegradation. its further conversion by all the strains proceeds via ortho-cleavage. the specific activity of catechol 1,2-dioxygenase assayed in crude extracts of rhodococcus sp. 400 and r. rhodochrous 172 with 3- and 4-methylcatechols does not exceed the enzyme activity assayed with catechol. two catechol 1,2-dioxygenas ... | 2009 | 19916910 |
| application of the second rule of transient-state kinetic isotope effects to an enzymatic mechanism. | the transient-state kinetic approach reveals the formation and subsequent interconversions of intermediates in real time. its potential for the mechanistic resolution of enzymatic and other complex chemical mechanisms has been severely limited however by the lack of a rigorous and applicable theoretical basis in contrast to that of the less direct but soundly based algebraic algorithms of the steady-state approach. having recently established three rigorously derived fundamental "rules" of trans ... | 2009 | 19924903 |
| role of interfacial tensions in the translocation of rhodococcus erythropolis during growth in a two phase culture. | rhodococcus erythropolis pr4 is an alkane-degrading bacterium, which grows well in media containing high concentrations of alkanes. these properties give the organism potential in the bioremediation of various environments contaminated by alkanes. in this study, we report the translocation of r. erythropolis pr4 from an aqueous phase to an alkane phase during growth in a two phase culture medium. when the alkane chain length was between c10 and c12, pr4 was located at the aqueous-alkane interfac ... | 2009 | 19924958 |
| comparison of the clinical, microbiological, radiological and haematological features of foals with pneumonia caused by rhodococcus equi and other bacteria. | the purpose of this study was to compare the clinical, microbiological, radiological, haematological and cytological features of foals with pneumonia caused by rhodococcus equi infection and with other bacteria, in order to provide markers for early diagnosis and treatment. a retrospective study of 113 cases of bacterial pneumonia was undertaken. although there was considerable overlap in the affected populations, foals with r. equi pneumonia were significantly younger and had higher respiratory ... | 2011 | 19932977 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| [promoter recognition and beta-galactosidase reporter gene expression in rhodococcus]. | the genus rhodococcus is of considerable interest in recent years, stemming from their diverse applications in biodegradation, bioremediation, biotransformation and biosurfactant. using nocardia/rhodococcus-escherichia coli shuttle plasmid pnv18.1 as the backbone vector, we tested the driven efficiency of promoters ptac and placz of e. coli and pami-1/pami-2 of r. ruber in host r. rhodochrous atcc 33278 by overexpression of nitrile hydratase. results showed that the specific activity of nitrile ... | 2009 | 19938479 |
| [particularities of alkane oxidation in rhodococcus erythropolis ek-1 strain--producer of surface-active substances]. | the hexadecane oxidation of rhodococcus erythropolls ek-1 was established to be catalyzed by the alkane hydroxylase complex containing fe-s proteid rubredoxin. alkane hydroxylase activity was increased twice in the presence of 10 mg/l iron ions in the cultivation medium. sodium ions were shown to be an activator of alkane hydroxylase. four types of alcohol dehydrogenases (nad, nadp, pyrroloquinoline quinone and n,n-dimethyll-4-nitrosoaniline-dependent enzymes) were found in hexadecane-grown cell ... | 2009 | 19938610 |
| isolation of alkane-degrading bacteria from deep-sea mediterranean sediments. | to isolate and identify alkane-degrading bacteria from deep-sea superficial sediments sampled at a north-western mediterranean station. | 2010 | 19943883 |
| the enhancement of biodesulfurization activity in a novel indigenous engineered pseudomonas putida. | the combustion of sulfur-rich fossil fuels leads to release of sulfur oxide pollution in the environment. in biodesulfurization process, an organism is able to remove sulfur from fossil fuels without decreasing the caloric value of those substrates. the main aim of this research was to design a recombinant microorganism to remove the highest amount of sulfur compounds in fossil fuels. | 2009 | 19946346 |
| bacteremia due to rhodococcus equi: a case report and review of the literature. | rhodococcus equi, previously known as corynebacterium equi, is one of the most important causes of zoonotic infections in grazing animals. increased cases of human infection with r. equi have been reported, especially in immunocompromised patients, within recent years. we present a case of r. equi bacteremia in a 51-year-old man with diabetes and liver cirrhosis, on long-term corticosteroid therapy after skin-grafting surgery. the patient recovered soon after he was treated with vancomycin. this ... | 2009 | 19946958 |
| biodegradation of the xenobiotic organic disulphide 4,4'-dithiodibutyric acid by rhodococcus erythropolis strain mi2 and comparison with the microbial utilization of 3,3'-dithiodipropionic acid and 3,3'-thiodipropionic acid. | application of the non-toxic 3,3'-thiodipropionic acid (tdp) and 3,3'-dithiodipropionic acid (dtdp) as precursors for the microbial production of polythioesters (ptes), a class of biologically persistent biopolymers containing sulphur in the backbone, was successfully established previously. however, synthesis of ptes containing 4-mercaptobutyrate (4mb) as building blocks could not be achieved. the very harmful 4mb is not used as a pte precursor or as the carbon source for growth by any known st ... | 2010 | 19959574 |
| immunity-related gene single nucleotide polymorphisms associated with rhodococcus equi infection in foals. | in previous work, we found significant associations of horse polymorphic microsatellite and immunity-related (ir) gene markers with rhodococcus equi infection of foals. here, a statistically significant association between a single nucleotide polymorphism (snp) within the interleukin 7 receptor-encoding gene (il7r) with high r. equi burden in transtracheal aspirates was found (fisher's f = 0.043, odds ratio: 8.00, 95% confidence interval: 1.127-56.795). further positional and/or functional candi ... | 2010 | 20002811 |
| a polyphasic study on the species diversity of the sediment microbiota of lake hévíz. | lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ... | 2009 | 20038486 |
| involvement of a novel abc transporter and monoalkyl phthalate ester hydrolase in phthalate ester catabolism by rhodococcus jostii rha1. | phthalate esters (pes) are important environmental pollutants. while the biodegradation of the parent compound, phthalate (pth), is well characterized, the biodegradation of pes is not well understood. in particular, prior to this study, genes involved in the uptake and hydrolysis of these compounds were not conclusively identified. we found that rhodococcus jostii rha1 could grow on a variety of monoalkyl pes, including methyl, butyl, hexyl, and 2-ethylhexyl pths. strain rha1 could not grow on ... | 2010 | 20038686 |
| catechol 1,2-dioxygenase from the gram-positive rhodococcus opacus 1cp: quantitative structure/activity relationship and the crystal structures of native enzyme and catechols adducts. | the first crystallographic structures of a catechol 1,2-dioxygenase from a gram-positive bacterium rhodococcus opacus 1cp (rho 1,2-ctd), a fe(iii) ion containing enzyme specialized in the aerobic biodegradation of catechols, and its adducts with catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol (benzene-1,2,3-triol), 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol and protocatechuate (3,4-dihydroxybenzoate) have been determined and analyzed. this study repre ... | 2010 | 20040374 |
| detection, expression and quantitation of the biodegradative genes in antarctic microorganisms using pcr. | in this study, 28 hydrocarbon-degrading bacterial isolates from oil-contaminated antarctic soils were screened for the presence of biodegradative genes such as alkane hydroxylase (alks), the ispalpha subunit of naphthalene dioxygenase (ndob), catechol 2,3-dioxygenase (c23do) and toluene/biphenyl dioxygenase (todc1/bpha1) by using polymerase chain reaction (pcr) methods. all naphthalene degrading bacterial isolates exhibited the presence of a 648 bp amplicon that shared 97% identity to a known nd ... | 2010 | 20043207 |
| degradation of 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol by rhodococcus imtechensis strain rkj300. | a bacterial strain rhodococcus imtechensis rkj300 (= mtcc 7085(t) = jcm 13270(t)) was isolated from pesticide-contaminated soil of punjab by the enrichment technique on minimal medium containing 4-nitrophenol. strain rkj300 is capable of utilizing 4-nitrophenol, 2-chloro-4-nitrophenol, and 2,4-dinitrophenol as sole sources of carbon and energy. the strain involved both oxidative and reductive catabolic mechanisms for initial transformation of these compounds. in the case of 2-chloro-4-nitropheno ... | 2010 | 20050667 |
| ex situ bioremediation of oil-contaminated soil. | an innovative bioprocess method, systematic environmental molecular bioremediation technology (sembt) that combines bioaugmentation and biostimulation with a molecular monitoring microarray biochip, was developed as an integrated bioremediation technology to treat s- and t-series biopiles by using the landfarming operation and reseeding process to enhance the bioremediation efficiency. after 28 days of the bioremediation process, diesel oil (tph(c10-c28)) and fuel oil (tph(c10-c40)) were degrade ... | 2010 | 20053499 |
| rhodococcus baikonurensis btm4c, a boron-tolerant actinobacterial strain isolated from soil. | by screening a bacterial population from the soil in tokyo, japan, we isolated a boron-tolerant bacterium, strain btm4c. strain btm4c grew under the boron excess conditions with 100 mm boric acid, which is generally toxic to bacteria. molecular phylogenetic, chemotaxonomic, and physiological data showed that the strain belongs to the genus rhodococcus, and is to be identified as rhodococcus baikonurensis. | 2010 | 20057133 |
| current understanding of the equine immune response to rhodococcus equi. an immunological review of r. equi pneumonia. | rhodococcus equi is recognised to cause chronic purulent bronchopneumonia in foals of less than 6 months of age. virulent strains of the bacteria possess a large 80-90 kb plasmid encoding several virulence-associated proteins, including virulence-associated protein a (vapa), which is associated with disease. r. equi pneumonia can represent significant costs and wastage to the equine breeding industry, especially on stud farms where the disease is endemic. this article reviews knowledge of the eq ... | 2010 | 20064668 |
| distribution of the 3-hydroxyl-3-methylglutaryl coenzyme a reductase gene and isoprenoid production in marine-derived actinobacteria. | during the course of our screening program to isolate isoprenoids from marine actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. these strains belonged to 21 different genera, but most were members of streptomyces, nocardia, rhodococcus, and micromonospora. some actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. therefore, we investigated whether these str ... | 2010 | 20067528 |
| [rhodococcus equi neumonia in a hiv infected patient: a case report]. | 2009 | 20067738 | |
| vaccination of mice with salmonella expressing vapa: mucosal and systemic th1 responses provide protection against rhodococcus equi infection. | conventional vaccines to prevent the pneumonia caused by rhodococcus equi have not been successful. we have recently demonstrated that immunization with salmonella enterica typhimurium expressing the vapa antigen protects mice against r. equi infection. we now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific iga, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation ... | 2010 | 20072623 |
| inhibitory effect of aged-petroleum hydrocarbons on the survival of inoculated microorganism in a crude-oil contaminated site. | we studied the effects of aged total petroleum hydrocarbons (aged tph) on the survival of allochthonous diesel-degrading rhodococcus sp. strain ys-7 in both laboratory and field investigations. the aged tph extracted from a crude oil-contaminated site were fractionized by thin-layer chromatography/flame ionization detection (tlc/fid). the three fractions identified were saturated aliphatic (sa), aromatic hydrocarbon (ah), and asphaltene-resin (ar). the ratio and composition of the separated frac ... | 2009 | 20075636 |
| benzylic and aryl hydroxylations of m-xylene by o-xylene dioxygenase from rhodococcus sp. strain dk17. | escherichia coli cells expressing rhodococcus dk17 o-xylene dioxygenase genes were used for bioconversion of m-xylene. gas chromatography-mass spectrometry analysis of the oxidation products detected 3-methylbenzylalcohol and 2,4-dimethylphenol in the ratio 9:1. molecular modeling suggests that o-xylene dioxygenase can hold xylene isomers at a kink region between alpha6 and alpha7 helices of the active site and alpha9 helix covers the substrates. m-xylene is unlikely to locate at the active site ... | 2010 | 20082074 |
| a thermally stable form of bacterial cocaine esterase: a potential therapeutic agent for treatment of cocaine abuse. | rhodococcal cocaine esterase (coce) is an attractive potential treatment for both cocaine overdose and cocaine addiction. coce directly degrades cocaine into inactive products, whereas traditional small-molecule approaches require blockade of the inhibitory action of cocaine on a diverse array of monoamine transporters and ion channels. the usefulness of wild-type (wt) cocaine esterase is hampered by its inactivation at 37 degrees c. herein, we characterize the most thermostable form of this enz ... | 2010 | 20086035 |
| biodegradation of pharmaceuticals by rhodococcus rhodochrous and aspergillus niger by co-metabolism. | this work investigated the possible fate of pharmaceuticals in the environment that are known to be resistant to biodegradation. a co-metabolism approach, adding a readily degradable carbon source, was used to study the biodegradation of some pharmaceuticals. the pharmaceuticals selected were all known to be micro pollutants and frequently used by humans. the microorganisms used primarily were rhodococcus rhodochrous, known to co-metabolize difficult to degrade hydrocarbons and aspergillus niger ... | 2010 | 20089297 |
| identification and characterization of bacteria isolated under selective pressure of volatile organic compounds. | several bacterial strains tolerant to volatile organic compounds (vocs) were isolated from the air emissions of a typical industrial wood painting plant. four strains that grew vigorously on a mineral salt basal medium and voc-saturated atmosphere were identified using biolog and fatty acids analysis. a 16s rdna sequence comparison was also performed on three of the four strains. their phylogenetic positions were respectively: mc8m6 of the genus arthrobacter mcep3 of the genus microbacterium, mc ... | 2009 | 20112870 |
| application of quantitative real-time pcr for quantification of rhodococcus sp. eh831 in a polyurethane biofilter. | rhodococcus sp. eh831 is a microbial species that can degrade volatile organic compounds. we optimized a method for monitoring quantitative real-time pcr (qrt-pcr) of eh831 that was incorporated into a polyurethane (pu) biofilter. when the genomic dna of eh831 was directly extracted from a pu sample with immobilized eh831, the recovery efficiency was very low due to dna absorption into the pu. dna amplification during pcr was also inhibited by pu impurities. therefore, a pre-treatment step was n ... | 2009 | 20112879 |
| mechanism of boron tolerance in soil bacteria. | boron (b) is toxic to living cells at levels above a certain threshold. we isolated several b-tolerant bacterial strains from soil samples and studied them for possible mechanisms of b tolerance. 16s rrna gene sequencing and comparative phylogenetic analysis demonstrated that the isolates belong to the following 6 genera: arthrobacter, rhodococcus, lysinibacillus, algoriphagus, gracilibacillus, and bacillus. these isolates exhibited b-tolerance levels of 80, 100, 150, 300, 450, and 450 mmol/l, r ... | 2010 | 20130690 |
| biosurfactant production by halotolerant rhodococcus fascians from casey station, wilkes land, antarctica. | isolate a-3 from antarctic soil in casey station, wilkes land, was characterized for growth on hydrocarbons. use of glucose or kerosene as a sole carbon source in the culture medium favoured biosynthesis of surfactant which, by thin-layer chromatography, indicated the formation of a rhamnose-containing glycolipid. this compound lowered the surface tension at the air/water interface to 27 mn/m as well as inhibited the growth of b. subtilis atcc 6633 and exhibited hemolytic activity. a highly hydr ... | 2010 | 20135319 |
| successful treatment of rhodococcus equi pneumonia in an adult horse. | 2010 | 20136716 | |
| rp-hplc/ms-apci analysis of odd-chain tags from rhodococcus erythropolis including some regioisomers. | we used reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (rp-hplc/ms-apci) for direct analysis of triacylglycerols (tags) from four different cultivations of rhodococcus erythropolis ccm 2595. this technique enabled us to identify and quantify the specific molecular species of tags directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. a total of 17 tags havi ... | 2010 | 20138031 |
| characterization of indigenous rhodococcus sp. 602, a strain able to accumulate triacylglycerides from naphthyl compounds under nitrogen-starved conditions. | an indigenous bacterium (strain 602) isolated in this study from a polluted soil sample collected in patagonia (argentina) was investigated in relation to its metabolic responses under unbalanced growth conditions. this strain was identified as rhodococcus sp. by molecular analyses. strain 602 showed the ability to degrade a wide range of compounds and to synthesize triacylglycerols under nitrogen-limiting conditions. cells were also able to accumulate triacylglycerols during cultivation on naph ... | 2010 | 20144706 |
| [infection due to rhodococcus equi in hiv infected patients: four cases]. | incidence of rhodococcus equi (r. equi) infection is increasing parallel to a greater prevalence of immunosupressed patients. this study aims to describe clinico-epidemiological characteristics of r. equi infections in a single hospital. | 2010 | 20144792 |
| hemolytic activity of a bacterial trehalose lipid biosurfactant produced by rhodococcus sp.: evidence for a colloid-osmotic mechanism. | a succinoyl trehalose lipid produced by rhodococcus sp. behaves as a biological surfactant and also displays various interesting biological activities. trehalose lipid has been shown to have a great tendency to partition into phospholipid membranes; therefore, the characterization of its interaction with biological membranes is of central importance. in this work, human red blood cells have been used as an experimental model. trehalose lipid causes the swelling of human erythrocytes followed by ... | 2010 | 20146489 |
| new vector system for random, single-step integration of multiple copies of dna into the rhodococcus genome. | we designed a new vector system for creating a random mutant library with multiple integrations of dna fragments into the rhodococcus genome in a single step. for this, we cotransformed two vectors into rhodococcus by electroporation: ptip-istab-sacb regulates the expression of the transposase (ista) and its helper protein (istb) under the influence of a thiostrepton-inducible promoter, and prtsk-sacb provides the transposable-marker dna. both are multicopy vectors that are stable in the host ce ... | 2010 | 20154109 |
| cholesterol utilization in mycobacteria is controlled by two tetr-type transcriptional regulators: kstr and kstr2. | mycobacterium tuberculosis is able to use a variety of carbon sources in vivo and current knowledge suggests that cholesterol is used as a carbon source during infection. the catabolized cholesterol is used both as an energy source (atp generation) and as a source of precursor molecules for the synthesis of complex methyl-branched fatty acids. in previous studies, we described a tetr-type transcriptional repressor, kstr, that controls the expression of a number of genes involved in cholesterol c ... | 2010 | 20167624 |
| infectious endocarditis caused by rhodococcus equi. | rhodococcus equi is an unusual cause of infection. furthermore, this infection also tends to be typically described in immunocompromised patients. this report describes a 25-year-old previously healthy man with infectious endocarditis that was found to have been caused by r equi complicated by a subarachnoid hemorrhage, subdural hematoma, and a superior mesenteric artery aneurysm. the patient was successfully treated with antibiotic therapy, followed by a resection of the superior mesenteric art ... | 2010 | 20172165 |
| evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification. | ionic liquid (il) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. more in-depth research involving environment-friendly il is much needed to explore pretreatment green route. in our case, il 1-methyl-3-methylimidazolium dimethylphosphite ([mmim]dmp) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. the pretreatment/saccharificati ... | 2010 | 20182710 |
| paternal uniparental isodisomy of chromosome 6 causing a complex syndrome including complete ifn-gamma receptor 1 deficiency. | mendelian susceptibility to mycobacterial disease (msmd) is a rare primary immunodeficiency associated with clinical disease caused by weakly virulent mycobacterial species. interferon gamma receptor 1 (ifn-gammar1) deficiency is a genetic etiology of msmd. we describe the clinical and genetic features of a 7-year-old italian boy suffering from msmd associated with a complex phenotype, including neonatal hyperglycemia, neuromuscular disease, and dysmorphic features. the child also developed necr ... | 2010 | 20186794 |
| [screening and denitrification characteristics of a heterotrophic nitrification-aerobic denitrifier bacteria]. | a heterotrophic nitrification-aerobic denitrifier bacteria cpz24 was isolated form the livestock wastewater by way of the limiting dilution combined with the chromogenic medium screening methods. this bacterium was gram positive, rod. the colonies of the strain were orange-red.it was identified as rhodococuus pyridinivorans according to its morphological and physiological properties and the analysis of its 16s rdna gene. studied on its function of heterotrophic nitrification and aerobic denitrif ... | 2009 | 20187396 |
| identification and characterization of genes involved in naphthalene degradation in rhodococcus opacus r7. | rhodococcus opacus r7 is a naphthalene-degrading microorganism which is also able to grow on o-xylene. this work describes the isolation and analysis of two new genomic regions in which genes involved in naphthalene (nar gene cluster) and salicylate (gen gene cluster) degradation are located. in the nar gene cluster we found: two genes encoding the large (naraa) and the small (narab) components of the naphthalene dioxygenase, three genes (rub1, rub2, rub1bis) encoding three rubredoxins, an orf ( ... | 2010 | 20195856 |
| effects of bovine milk lactoperoxidase system on some bacteria. | bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ... | 2010 | 20198919 |
| contribution of extracellular polymeric substances on representative gram negative and gram positive bacterial deposition in porous media. | the significance of extracellular polymeric substances (eps) on cell transport and retained bacteria profiles in packed porous media (quartz sand) was examined by direct comparison of the overall deposition kinetics and retained profiles of untreated bacteria (with eps) versus those of treated cells (without eps) from the same cell type. four representative cell types, pseudomonas sp. qg6 (gram-negative, motile), mutant escherichia coli bl21 (gram-negative, nonmotile), bacillus subtilis (gram-po ... | 2010 | 20201559 |
| hydrolysis of benzonitrile herbicides by soil actinobacteria and metabolite toxicity. | the soil actinobacteria rhodococcus rhodochrous pa-34, rhodococcus sp. ndb 1165 and nocardia globerula nhb-2 grown in the presence of isobutyronitrile exhibited nitrilase activities towards benzonitrile (approx. 1.1-1.9 u mg(-1) dry cell weight). the resting cell suspensions eliminated benzonitrile and the benzonitrile analogues chloroxynil (3,5-dichloro-4-hydroxybenzonitrile), bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) and ioxynil (3,5-diiodo-4-hydroxybenzonitrile) (0.5 mm each) from reacti ... | 2010 | 20204468 |
| trypanosoma cruzi: synergistic cytotoxicity of multiple amphipathic anti-microbial peptides to t. cruzi and potential bacterial hosts. | the parasite trypanasoma cruzi is responsible for chagas disease and its triatomine vector, rhodnius prolixus, has a symbiotic relationship with the soil bacterium, rhodococcus rhodnii. r. rhodnii that was previously genetically engineered to produce the anti-microbial peptide, cecropin a was co-infected with t. cruzi into r. prolixus resulting in clearance of the infectious t. cruzi in 65% of the vectors. similar anti-microbial peptides have been isolated elsewhere and were studied for differen ... | 2010 | 20206169 |
| biotransformation of amides to acids using a co-cross-linked enzyme aggregate of rhodococcus erythropolis amidase. | rhodococcus erythropolis amidase was expressed in escherichia coli cells. the crude amidase in the cell-free extract was immobilized using the cross-linked enzyme aggregate (clea) method. the crude amidase was mixed with bovine serum albumin and then precipitated with ammonium sulfate. the resultant precipitant was subsequently cross-linked with glutaraldehyde. scanning electron microscopy revealed that this co-clea had a ball-like shape of a diameter of approximately 1 micronm. this co-clea evi ... | 2010 | 20208436 |
| engineering cytochrome p450 monooxygenase cyp 116b3 for high dealkylation activity. | cytochrome p450 monooxygenase cyp116b3 from rhodococcus ruber catalyzes the dealkylation of 7-ethoxycoumarin and the hydroxylation of substituted and unsubstituted aromatics. however, since activities were quite low, a combination of site-specific mutagenesis and directed evolution was applied to produce 7800 variants of cyp116b3, which were screened via a newly developed high-throughput screening system based on the dealkylation of 7-ethoxycoumarin catalyzed by recombinant e. coli. the best mut ... | 2010 | 20213524 |
| identification of atypical rhodococcus-like clinical isolates as dietzia spp. by 16s rrna gene sequencing. | rhodococcus equi and dietzia spp. are closely related actinomycetes that show similar phenotypic properties. in humans, r. equi is an opportunistic pathogen associated with severe immunodeficiency. dietzia spp. are environmental bacteria that have been isolated recently from clinical material and are presumptively associated with human infections. during the last 5 years, 15 bacterial isolates from human clinical samples collected at the hospital marqués de valdecilla, santander, spain, were ide ... | 2010 | 20220156 |
| kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase. | nitrile hydratases (nhase), which catalyze the hydration of nitriles to amides, have an unusual fe(3+) or co(3+) center with two modified cys ligands: cysteine sulfininate (cys-so(2) (-)) and either cysteine sulfenic acid or cysteine sulfenate [cys-so(h)]. two catalytic mechanisms have been proposed. one is that the sulfenyl oxygen activates a water molecule, enabling nucleophilic attack on the nitrile carbon. the other is that the ser ligand ionizes the strictly conserved tyr, activating a wate ... | 2010 | 20221653 |
| identification of rhodococcus equi lipids recognized by host cytotoxic t lymphocytes. | immune adult horses have cd8(+) cytotoxic t lymphocytes (ctls) that recognize and lyse rhodococcus equi-infected cells in an equine lymphocyte alloantigen (ela)-a [classical major histocompatibility complex (mhc) class i]-unrestricted fashion. as protein antigens are mhc class i-restricted, the lack of restriction suggests that the bacterial antigens being recognized by the host are not proteins. the goals of this study were to test the hypothesis that these ctls recognize unique r. equi cell-wa ... | 2010 | 20299405 |
| expression of the avian-specific toll-like receptor 15 in chicken heterophils is mediated by gram-negative and gram-positive bacteria, but not tlr agonists. | toll-like receptors (tlrs) are a critical component of the innate immune response of mammalian and avian species. while most mammalian tlrs have been well characterized, the chicken-specific tlr15 has not been extensively studied. we recently demonstrated that tlr15 is differentially expressed between salmonella-susceptible-and-resistant chickens, indicating a potential role in the innate immune response to infection with salmonella. the aim of the present study was to gain better insight into t ... | 2010 | 20303182 |
| recurrent rhodococcus gordoniae eyelid infection after combined septoplasty and blepharoplasty. | rhodococcus species are rare human pathogens. most cases of human infection have involved rhodococcus equi, a natural zoonotic pathogen causing pneumonia in young foals. the human population at risk mainly includes immunocompromised patients. infection of immunocompetent hosts has been rare, with less than 30 reported cases in the literature. the authors report a first case of recurrent rhodococcus gordoniae granulomatous lower eyelid infection that occurred after an immunocompetent 44-year-old ... | 2010 | 20305518 |
| identification of immunologically relevant genes in mare and foal dendritic cells responding to infection by rhodococcus equi. | rhodococcus equi is a facultative intracellular bacterial pathogen of horses; infected foals develop pyogranulomatous pneumonia, however adult horses are largely unaffected. r. equi infects and proliferates within host macrophages and dendritic cells (dcs). dcs initiate the appropriate adaptive immune response, thereby playing a critical role in determining the outcome of infection. our aim was to identify genes that are differentially expressed in r. equi infected monocyte-derived dcs (mddcs). ... | 2010 | 20334935 |
| cytochromes p450-mediated degradation of fuel oxygenates by environmental isolates. | the degradation of fuel oxygenates [methyl tert-butyl ether (mtbe), ethyl tert-butyl ether (etbe) and tert-amyl methyl ether (tame)] by rhodococcus ruber ifp 2001, rhodococcus zopfii ifp 2005 and gordonia sp. ifp 2009 (formerly mycobacterium sp.) isolated from different environments was compared. strains ifp 2001, ifp 2005 and ifp 2009 grew on etbe due in part to the activity of a cytochrome p450, cyp249. all of these strains were able to degrade etbe to tert-butyl alcohol and are harboring the ... | 2010 | 20337704 |
| site-directed mutagenesis enhances the activity of nadh-fmn oxidoreductase (dszd) activity of rhodococcus erythropolis. | microbial desulfurization is potentially an alternative process to chemical desulfurization of fossil fuels and their refined products. the dibenzothiophene desulfurizing system of rhodococcus erythropolis includes dszd which is an nadh-dependent fmn oxidoreductase with 192 residues that is responsible for supplying reducing equivalents in the form of fmnh(2) to monooxygenases, dsza and dszc. we performed amino acid sequence comparisons and structural predictions based on the crystal structure o ... | 2010 | 20349330 |
| [cloning, heterologous expression and purification of a 3-ketosteroid-9alpha-hydroxylase (ksh) from mycobacterium sp. nwib-01]. | 3-ketosteroid-9alpha-hydroxylase (ksh), a key enzyme in the microbial steroid degradation, is highly valuable for the production of some steroid drugs. degenerate primers were designed by comparing the ksh from rhodococcus erythropolis sq1 and its homologous sequences in the reported genome of mycobacteria. subsequently, a gene fragment of ksh was cloned from mycobacterium sp. nwib-01, a sterol-transforming bacterium isolated from soil in our lab. according to the conservative sequence, the full ... | 2009 | 20352983 |