Publications

simultaneous quantitative determination method for sphingolipid metabolites by liquid chromatography/ionspray ionization tandem mass spectrometry.	sphingolipid metabolites ceramide, sphingomyelin, sphingosine, psycosine, sphingosylphosphorylcholine, and dimethylsphingosine were separated and simulataneously quantitated by liquid chromatography/ionspray ionization tandem mass spectrometry (lc/ms/ ms). the use of glassware throughout minimized losses due to adsorption and the pretreatment of this method consisted of simple liquid-liquid extraction procedure with a mixture of chloroform and methanol. after separation on a short c18 silica col ...	1997	9025946
role of hemolytic and nonhemolytic phospholipase c from pseudomonas aeruginosa for inflammatory mediator release from human granulocytes.	pseudomonas aeruginosa phospholipase c (plc) is a critical component in the pathogenesis of severe p. aeruginosa infections. however, p. aeruginosa can produce a hemolytic (plc-h) as well as a nonhemolytic (plc-n) variant, both having a mw of about 77 kd. in the past, studies did not distinguish between both types of plc with regard to the induction of inflammatory mediators from human cells.	1997	9030090
[oral long-term administration of probiotic b. cereus--an alternative for the the prevention of enterotoxemia?].	aetiology and epizootiology of enterotoxaemia, especially the connection between development of the disease and the management of feeding are described and newer knowledge about the physiopathology of the disease is presented. a comparative description of prophylactic schemes which include immuno- and chemoprophylaxis follows. the principles of the application and the effect of probiotic organisms as a new approach to prevent the disease are explained. the probiotic effect of apathogenic non-hae ...	1996	9035967
the occurrence of bacillus cereus in danish pasteurized milk.	four hundred and fifty eight samples of pasteurized full fat milk, pasteurized low fat milk (1.5% milk fat) and pasteurized double cream were collected from three danish dairies (a, b and c) over a period of 1 year. the milk samples were stored at 7 +/- 0.5 degrees c for 8 days, and were then examined for the presence of bacillus cereus and other aerobic mesophilic microorganisms. in addition, 115 raw milk samples taken from weighing tanks at the three dairies were examined for psychrotrophic b. ...	1997	9039564
isolation and characterisation of bacillus cereus from pasteurised milk in household refrigerators in the netherlands.	the incidence and some characteristics (carbohydrate metabolism, growth profiles, haemolysin production and enterotoxin production) of bacillus cereus, in pasteurised, low-fat (1.5%) milk, in household refrigerators in the netherlands was investigated. in 247 (74%) of the 334 milk samples analyzed, the mesophilic aerobic counts were between 50 and 5000 per millilitre. b. cereus could be isolated from 133 (40%) of the samples. in general the b. cereus counts were low; numbers of less than five pe ...	1997	9039575
cloning of novel enterotoxin genes from bacillus cereus and bacillus thuringiensis.	a novel enterotoxin gene was cloned from bacillus cereus fm1, and its nucleotide sequence was determined. previously, a 45-kda protein causing characteristic enterotoxin symptoms in higher animals had been isolated (k. shinagawa, p. 181-193, in a. e. pohland et al., ed., microbial toxins in foods and feeds, 1990) from the same b. cereus strain, but no report of cloning of the enterotoxin gene has been published. in the present study, a specific antibody to the purified enterotoxin was produced a ...	1997	9055420
maltodextrin stimulates growth of bacillus cereus and synthesis of diarrheal enterotoxin in infant milk formulae.	one hundred reconstituted milk-based infant formulae (imf) representative of 10 leading brands available in many european economic community countries were examined for bacillus cereus and for the presence of diarrheal enterotoxin. sixty-three reconstituted imf supported growth of the organism after 14 h at 25 degrees c, and in 4 of these, which contained maltodextrin, enterotoxin was detected. reconstituted imf (and basal synthetic media) supplemented with > or = 0.1% maltodextrin supported bot ...	1997	9055435
synthesis and use of a substrate for the detection of isopeptidase activity.	we have developed a substrate to assay for an isopeptidase, an enzyme capable of cleaving the nepsilon-(gamma-glutamic) lysine bond which crosslinks polypeptide chains. this substrate consists of modified lysine (n-alpha-[3h]acetyl-l-lysine-n-methylamide or alma), linked by its epsilon-amino group to a gamma-carboxyl amide group of casein with guinea pig liver transglutaminase or factor xiiia. we used this substrate to demonstrate the release of [3h]alma from [3h]alma-casein in a culture medium ...	1997	9056190
pyrolysis mass spectrometry studies on bacillus anthracis, bacillus cereus and their close relatives.	pyrolysis mass spectrometry was used to examine strains of b. anthracis, of b. cereus, of b.cereus either proven to cause emetic illness or connected with outbreaks of emetic food poisoning and of b.thuringiensis. analysis of the data-set for all strains allowed differentiation between b.anthracis, the emetic b.cereus and b.thuringiensis but b.cereus strains could not be clearly discriminated. removal of data for the b.thuringiensis and the emetic b.cereus strains, followed by re-analysis, allow ...	1997	9060166
survival of selected bacteria and fungi in hypertonic (7.2%) saline.		1997	9066973
necrotizing bacillus cereus infection of the meninges without inflammatory reaction in a patient with acute myelogenous leukemia: a case report.	a 64-year-old man in a severely immunocompromised state due to acute myelogenous leukemia died, respirator-unaided, about 10 h after the abrupt onset of coma. an earlier blood culture had yielded bacillus cereus. the autopsy, performed 2 h after death, demonstrated diffuse subarachnoid hemorrhage without berry aneurysms, and the formalin-fixed brain was tinged with gray-brownish discoloration. the sections of the brain presented a whitish tint of the surface layer of all portion of the cerebral ...	1997	9083563
sensitivity of aeromonas hydrophila carbapenemase to delta3-cephems: comparative study with other metallo-beta-lactamases.	ceftriaxone and ceftriaxone s-oxide behaved as inactivators against the metallo-beta-lactamase of aeromonas hydrophila ae036 and as substrates for the zinc beta-lactamase produced by bacillus cereus (569/h/9) and stenotrophomonas maltophilia ula 511. moreover, ro 09-1428, a catechol-cephalosporin, was not recognized by the a. hydrophila enzyme. panipenem, cephalosporin c, cephalosporin c-gamma-lactone, and loracarbef were substrates for the three studied beta-lactamases.	1997	9087509
expression of neutral sphingomyelinase identifies a distinct pool of sphingomyelin involved in apoptosis.	the activation of sphingomyelinase and generation of ceramide have been implicated as important regulatory pathways in cell growth and apoptosis. bacterial sphingomyelinase has been used in many cell systems to mimic the activation of endogenous sphingomyelinase. these studies, however, have been complicated by the inability of exogenously applied bacterial sphingomyelinase to perform many of the effects of short chain cell permeable ceramides, indicating that there may be a distinct signal tran ...	1997	9092485
molecular cloning and characterization of the genes encoding the l1 and l2 components of hemolysin bl from bacillus cereus.	hemolysin bl, which is composed of a binding component, b, and two lytic components, l1 and l2, is the enterotoxin responsible for the diarrheal food poisoning syndrome caused by strains of bacillus cereus. to further characterize the toxin, we sought to clone and sequence the genes encoding the l1 and l2 proteins. a genomic library was screened with polyclonal antibody to the l1 and l2 proteins to identify recombinant clones containing the genes. five clones reacted with the antibody to l2, but ...	1997	9098052
fulminant liver failure in association with the emetic toxin of bacillus cereus.	a 17-year-old boy and his father had acute gastroenteritis after eating spaghetti and pesto that had been prepared four days earlier. within two days, fulminant liver failure and rhabdomyolysis developed in the boy and he died. the father had hyperbilirubinemia and rhabdomyolysis but recovered. we investigated the cause of these illnesses.	1997	9099658
power failure, liver failure.		1997	9099663
[formation of a resting form of bacillus cereus and micrococcus luteus].	under certain cultivation conditions, the bacteria bacillus cereus and micrococcus luteus form cystlike refractive cells (up to 60% of the total number) that retain viability over a long time, are metabolically inactive and thermotolerant and possess specific ultrastructure. these properties allow them to be attributed to a new type of resting forms of microorganisms.	1996	9102554
[the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course].	a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ...	1996	9103069
analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by high-performance liquid chromatography.	a novel method for the determination of dipalmitoyl phosphatidylcholine (dppc) in amniotic fluid by high-performance liquid chromatography (hplc) is described. aliquots of 50 microliters of amniotic fluid were hydrolyzed with phospholipase c from bacillus cereus and the resulting dipalmitoylglycerol analyzed by hplc. run-to-run and day-to-day precision for dppc analysis were 4.2 and 6.1%, respectively, and analysis time was 10 min. recoveries for dppc ranged between 92 and 98%. in summarizing, t ...	1997	9106062
involvement of phosphatidylcholine-specific phospholipase c in platelet-derived growth factor-induced activation of the mitogen-activated protein kinase pathway in rat-1 fibroblasts.	the role of phosphatidylcholine (pc) hydrolysis in activation of the mitogen-activated protein kinase (mapk) pathway by platelet-derived growth factor (pdgf) was studied in rat-1 fibroblasts. pdgf induced the transient formation of phosphatidic acid, choline, diacylglycerol (dg), and phosphocholine, the respective products of phospholipase d (pld) and phospholipase c (pc-plc) activity, with peak levels at 5-10 min. pld-catalyzed transphosphatidylation (with n-butyl alcohol) diminished dg formati ...	1997	9110992
reversion of ras- and phosphatidylcholine-hydrolyzing phospholipase c-mediated transformation of nih 3t3 cells by a dominant interfering mutant of protein kinase c lambda is accompanied by the loss of constitutive nuclear mitogen-activated protein kinase/extracellular signal-regulated kinase activity.	the transformed phenotype of v-ras- or bacillus cereus phosphatidylcholine-hydrolyzing phospholipase c (pc-plc)-expressing nih 3t3 cells is reverted by expressing a kinase-defective mutant of protein kinase c lambda (lambdapkc). we report here that extracellular signal-regulated kinase (erk)-1 and -2 are constitutively activated in v-ras- and pc-plc-transformed cells in the absence of added growth factors. interestingly, the activated erks were exclusively localized to the cell nucleus. consiste ...	1997	9111071
food-borne disease outbreaks due to bacteria in taiwan, 1986 to 1995.	between 1986 and 1995, 852 outbreaks of food-borne disease involving 26,173 cases and 20 deaths were reported in taiwan. about 80% of the outbreaks occurred in the warmer months, i.e., between april and october. of the 852 reported outbreaks, 555 (65%) were caused by bacterial pathogens. the three most common bacteria involved were vibrio parahaemolyticus (35%, 197 of 555 outbreaks), staphylococcus aureus (30%, 169 of 555 outbreaks), and bacillus cereus (18%, 104 of 555 outbreaks).	1997	9114420
rapid identification of bacterially contaminated platelets using reagent strips: glucose and ph analysis as markers of bacterial metabolism.	one in every 1000 units of platelets is bacterially contaminated, which puts patients at risk for transfusion-associated sepsis and death. however, there is currently no screening test in place to detect contaminated units. the use of commercially available multiple-reagent urine dipsticks for this purpose was evaluated.	1997	9122896
evidence from whole-sediment, porewater, and elutriate testing in toxicity assessment of contaminated sediments.	bioassays with microorganisms are widely used as tools in estimating the potential risk of contaminated sediments. although whole-sediment testing seems the most realistic approach to evaluate the bioavailability of contaminants in sediments, porewaters and aqueous extracts are frequently used. the object of this study was to compare whole sediment, porewater, and elutriate exposure of contaminated sediment simultaneously with two bacterial bioassays. artificial sediment was spiked with five che ...	1997	9126431
[bacterial cell membrane atpase in assessing heavy metal toxicity].	sensitivity of membrane atpase to heavy metals was studied in bacterial stains bacillus cereus atcc 14579, bacillus cereus b4368, and alcaligenes eutrophus ch34 was studied. the inhibition effects of metals on membrane atpase ranged in the same order (au > cu > zn > co > mn) as their effects on the growth of cultures. it is suggested that membrane atpase is one of the targets for the action of heavy metal ions within the microbial cell and, therefore, its activity may serve as an indicator of th ...	1997	9132529
[formation of resting cells in microbial suspensions undergoing autolysis].	under experimentally selected conditions favoring spontaneous or induced autolysis of cell suspensions, the asporogenous bacteria escherichia coli and methylococcus capsulatus, the bacilli bacillus cereus (under conditions of suppressed sporulation), and the yeast saccharomyces cerevisiae were shown to be capable of forming cystlike resting cells. their number was influenced by (1) cell density in the suspensions; (2) the presence of ca2+ ions in nutrient-limited medium; (3) ph of medium; and (4 ...	1997	9132531
three bacillus cereus bacteriophage endolysins are unrelated but reveal high homology to cell wall hydrolases from different bacilli.	the ply genes encoding the endolysin proteins from bacillus cereus phages bastille, tp21, and 12826 were identified, cloned, and sequenced. the endolysins could be overproduced in escherichia coli (up to 20% of total cellular protein), and the recombinant proteins were purified by a two-step chromatographical procedure. all three enzymes induced rapid and specific lysis of viable cells of several bacillus species, with highest activity on b. cereus and b. thuringiensis. ply12 and ply21 were expe ...	1997	9139898
bacillus cereus may produce two or more diarrheal enterotoxins.	bacillus cereus strains were tested for production of diarrheal enterotoxin by the reverse passive latex agglutination test and for presence of b. cereus enterotoxin gene (bcet) by polymerase chain reaction. about 50% of 56 b. cereus strains reacted positive in broth culture in the reverse passive latex agglutination test, while the bcet gene was detected in 41.1%. sixteen percent of the strains were positive for both diarrheal enterotoxin and bcet gene. a 741 bp probe prepared from the polymera ...	1997	9141665
effects of nisin and temperature on survival, growth, and enterotoxin production characteristics of psychrotrophic bacillus cereus in beef gravy.	the presence of psychrotrophic enterotoxigenic bacillus cereus in ready-to-serve meats and meat products that have not been subjected to sterilization treatment is a public health concern. a study was undertaken to determine the survival, growth, and diarrheal enterotoxin production characteristics of four strains of psychrotrophic b. cereus in brain heart infusion (bhi) broth and beef gravy as affected by temperature and supplementation with nisin. a portion of unheated vegetative cells from 24 ...	1997	9143127
fulminant septicemic syndrome of bacillus cereus in a leukemic patient.	we report a rapidly fatal bacillus cereus septicemia in a leukemic patient receiving remission-induction therapy. symptoms resembling food poisoning and fever preceded coma accompanied by neurologic abnormalities. autopsy revealed necrotizing leptomeningitis with subarachnoid hemorrhage and coagulation necrosis of the liver with bacterial infiltration. these clinicopathologic findings were closely similar to those of reported cases. because of a rapidly fatal clinical course, suspicion of this s ...	1997	9144019
synthesis and biological evaluation of monoindolyl and indolocarbazolyl oxazolones and imidazolones.	eight compounds structurally related to protein kinase c inhibitor mdl 27032 and substituted with indole moieties were synthesized. their activities towards protein kinase c (pkc) and protein kinase a (pka) were determined. their effect on pkc-mediated contraction of rat tracheal smooth muscle, their antiproliferative activity on two murine tumor cell lines, melanoma b16 and leukemia p388 and their antimicrobial activity on a gram-positive bacterium bacillus cereus were also examined. the mammal ...	1997	9145508
antibacterial effect of the adhering human lactobacillus acidophilus strain lb.	the spent culture supernatant of the human lactobacillus acidophilus strain lb produces an antibacterial activity against a wide range of gram-negative and gram-positive pathogens. it decreased the in vitro viability of staphylococcus aureus, listeria monocytogenes, salmonella typhimurium, shigella flexneri, escherichia coli, klebsiella pneumoniae, bacillus cereus, pseudomonas aeruginosa, and enterobacter spp. in contrast, it did not inhibit lactobacilli and bifidobacteria. the activity was heat ...	1997	9145867
the bacteriological quality of hospital-prepared infant feeds.	twenty-four pasteurized infant feeds, prepared in a glasgow hospital, were examined microbiologically. all produced a satisfactory total aerobic mesophilic count of < or = 1.0 x 10(4) cfu/g (mean 6.3 x 10(1) cfu/g) within 1 h of preparation. bacillus cereus was detected in two infant feeds immediately after preparation and one of these had a b. cereus count of 1.4 x 10(3) cfu/g exceeding the recommended safety limit of < or = 1.0 x 10(3) cfu/g. subsequent storage over a 14 h period at 25 degrees ...	1997	9152818
model building of a thermolysin-like protease by mutagenesis.	the present study concerns the use of site-directed mutagenesis experiments to optimize a three-dimensional model of the neutral protease of bacillus subtilis (np-sub). an initial model of np-sub was constructed using the crystal structures of the homologous neutral proteases of bacillus thermoproteolyticus (thermolysin) and bacillus cereus as templates. the largest portion of np-sub could be modelled satisfactorily, using standard techniques, but several surface-located regions could only be mo ...	1997	9153087
sterilisation of surgical instruments with formaldehyde gas.	haemostatic forceps contaminated with pseudomonas aeruginosa, staphylococcus aureus, bacillus cereus and aspergillus flavus were exposed to formaldehyde gas for up to 48 hours at different temperatures and relative humidities. after 24 hours at 22 to 24 degrees c and a relative humidity of 73 per cent they were sterilised reliably and completely.	1997	9153745
the role of the nonconserved residues at position 167 of class a beta-lactamases in susceptibility to mechanism-based inhibitors.	differences in specificities between the class a beta-lactamases for both substrate and inhibitors are known. the role of the nonconserved amino acid residue at position 167 of the class a enzyme, which forms a cis bond with the catalytically essential glu-166 residue, in both the hydrolysis of beta-lactam substrates and inactivation by mechanism-based inhibitors, was investigated. site-directed mutagenesis was performed on the penpc gene encoding the bacillus cereus 569/h beta-lactamase i to re ...	1996	9158770
time to b. cereus about hot chocolate.	to determine the cause of illnesses experienced by employees of a minneapolis manufacturing plant after drinking hot chocolate bought from a vending machine and to explore the prevalence of similar vending machine-related illnesses.	1997	9160059
mutual modulation of sphingomyelinase and phospholipase a2 activities against mixed lipid monolayers by their lipid intermediates and glycosphingolipids.	sphingomyelinase activity against pure sphingomyelin monolayers is constant up to a surface pressure of 18 mn/m and falls above it. sphingomyelinase- and phospholipase a2-mediated phosphohydrolytic pathways are mutually modulated by the presence of their respective substrates and products. at 15 mn/m non-substrate lipids such as ceramide at a mole fraction of 0.1 in mixed films with the pure substrate, inhibit the sphingomyelinase activity. ganglioside gm1, another ceramide-containing complex sp ...	1997	9160338
use of pulsed-field gel electrophoresis to investigate a pseudo-outbreak of bacillus cereus in a pediatric unit.	bacillus cereus is a well-known cause of food poisoning. it also causes rare systemic infections, usually in immunocompromised patients. dissemination of this species in hospitals had been reported. most of these episodes were pseudo-outbreaks and were usually secondary to equipment or environmental contamination. we report here on the use of pulsed-field gel electrophoresis (pfge) to analyze a pseudo-outbreak of b. cereus in a pediatric unit. different restriction endonucleases had been tested, ...	1997	9163476
bacterial viability and antibiotic susceptibility testing with sytox green nucleic acid stain.	a fluorescent nucleic acid stain that does not penetrate living cells was used to assess the integrity of the plasma membranes of bacteria. sytox green nucleic acid stain is an unsymmetrical cyanine dye with three positive charges that is completely excluded from live eukaryotic and prokaryotic cells. binding of sytox green stain to nucleic acids resulted in a > 500-fold enhancement in fluorescence emission (absorption and emission maxima at 502 and 523 nm, respectively), rendering bacteria with ...	1997	9172364
[bacillus cereus pneumonia after thoracic trauma. case report and review of the literature].	we report on the case of a pneumonia caused by bacillus cereus in a patient who sustained severe thoracic trauma, fractures of ribs 3-12, left lung contusion and haematopneumothorax. bronchoscopic alveolar lavage (bal) performed on icu day four helped identifying the underlying cause of pneumonia. inspite of early diagnosis and antibiotic treatment with clindamycin, cefotaxim and tobramycin, which rapidly eliminated the bacteria from the patient's lungs, the patient eventually died from the seve ...	1997	9172719
cloning, sequencing and overexpression of the leucine dehydrogenase gene from bacillus cereus.	the l-leucine dehydrogenase gene from bacillus cereus (dsm 626) was cloned from a partial genomic library and sequenced. the open reading frame has 1101 bp and codes for a protein of 39.9 kda. the deduced amino acid sequence of the leudh from b. cereus shares 70-80% identity with leudh's from the thermophilic strains b. stearothermophilus and thermoactinomyces intermedius. the active protein was overexpressed in escherichia coli to yield approximately 30% of the total soluble protein.	1997	9188201
intracellular transport and processing of protein toxins produced by enteric bacteria.	bacterial toxins are associated with disease in humans and animals. toxins can either be preformed in food or produced by bacteria in the intestine. there are two types of toxins: heat-labile protein toxins and heat stabile toxins. heat labile toxins are produced by bacillus cereus, clostridium perfringens, escherichia coli, and vibrio cholerae, and heat-stabile enterotoxins consisting of relatively few amino acids are produced by escherichia coli and acts by activation of guanylate cyclase. sim ...	1997	9192018
the refined crystal structure of bacillus cereus oligo-1,6-glucosidase at 2.0 a resolution: structural characterization of proline-substitution sites for protein thermostabilization.	the crystal structure of oligo-1,6-glucosidase (dextrin 6-alpha-glucanohydrolase, ec 3.2.1.10) from bacillus cereus atcc7064 has been refined to 2.0 a resolution with an r-factor of 19.6% for 43,328 reflections. the final model contains 4646 protein atoms and 221 ordered water molecules with respective root-mean-square deviations of 0.015 a for bond lengths and of 3.166 degrees for bond angles from the ideal values. the structure consists of three domains: the n-terminal domain (residues 1 to 10 ...	1997	9193006
[meningoencephalitis caused by bacillus cereus].		1997	9198505
the use of bacillus diarrhoeal enterotoxin (bde) detection using an elisa technique in the confirmation of the aetiology of bacillus-mediated diarrhoea.	a commercially available elisa kit was used for the detection of bacillus diarrhoeal enterotoxin (bde) in a variety of foods and faeces. the ability of isolates of bacillus spp., including bacillus cereus, to produce bde in brain heart infusion broth containing 0.1% glucose was also checked by use of the kit. results show that 29 out of 31 b. cereus isolates were enterotoxigenic. foods positive for performed bde were always contaminated with > 10(5) b. cereus cfu g-1, but not all foods contamina ...	1997	9202434
effect of high-power microwave on indicator bacteria for sterilization.	according to the superiority sterilization, a specially-designed microwave disinfector using high-power microwave energy was made. a series of sterilizing experiments have been made to determine the effect of microwave energy on several typical indicator bacteria such as bacillus subtilis var. niger, bacillus stearothermophilus, bacillus pumilus e601, staphylococcus aureus, bacillus cereus. under the conditions of different sterilization duration and unequal intensity of microwave power irradiat ...	1996	9216147
precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase.	a genetic block was introduced in the first condensation step of the polyketide biosynthetic pathway that leads to the formation of 6-deoxyerythronolide b (6-deb), the macrocyclic precursor of erythromycin. exogenous addition of designed synthetic molecules to small-scale cultures of this null mutant resulted in highly selective multimilligram production of unnatural polyketides, including aromatic and ring-expanded variants of 6-deb. unexpected incorporation patterns were observed, illustrating ...	1997	9219693
acquired carbapenemases.		1997	9222034
in-vitro activity of trovafloxacin against clinical bacterial isolates from patients with cancer.	the antibacterial activity of trovafloxacin was compared with that of ciprofloxacin, levofloxacin, ofloxacin, sparfloxacin and norfloxacin against bacterial isolates from patients with cancer. in general, the activity of trovafloxacin was comparable to that of ciprofloxacin, levofloxacin and sparfloxacin against most gram-negative isolates tested (minor differences in the activity of each agent against individual species were seen) and it was the most active agent tested against stenotrophomonas ...	1997	9222065
channelling of deoxyribose moiety of exogenous dna into carbohydrate metabolism: role of deoxyriboaldolase.	in bacteria, the addition of (deoxy)nucleosides or (deoxy)ribose to the growth medium causes induction of enzymes involved in their catabolism, leading to the utilisation of the pentose moiety as carbon and energy source. in this respect, deoxyriboaldolase appears the key enzyme, allowing the utilisation of deoxyribose 5-p through glycolysis. we observed that not only deoxynucleosides, but also dna added to the growth medium of bacillus cereus induced deoxyriboaldolase; furthermore, the switch o ...	1997	9226884
platelet-derived growth factor activation of mitogen-activated protein kinase depends on the sequential activation of phosphatidylcholine-specific phospholipase c, protein kinase c-zeta and raf-1.	the mechanism of raf-1 activation by platelet-derived growth factor (pdgf) is poorly defined. we previously reported that, in rat-1 fibroblasts, pdgf activates a phosphatidylcholine-specific phospholipase c (pc-plc) and that the product, diacylglycerol, somehow activates protein kinase c-zeta (pkc-zeta). both pc-plc and pkc-zeta activities were required for pdgf activation of mitogen-activated protein kinase (mapk). now we report that mapk activation by exogenous pc-plc depends on raf-1 activati ...	1997	9230106
site-specific mutagenesis of clostridium perfringens alpha-toxin: replacement of asp-56, asp-130, or glu-152 causes loss of enzymatic and hemolytic activities.	the current study has investigated the role of d-56, d-130, and e-152 in zinc ion binding properties, as well as the hemolytic, phospholipase c (plc), and sphingomyelinase (smase) activities of clostridium perfringens alpha-toxin, based upon crystallography studies of the bacillus cereus plc, which had suggested these residues might be important for these functional activities. the replacement of d-56 in alpha-toxin resulted in complete loss of hemolytic, plc, and smase activities. the variant t ...	1997	9234819
bacteriologic studies of skin, tissue fluid, lymph, and lymph nodes in patients with filarial lymphedema.	filarial lymphedema is complicated by frequent episodes of dermatolymphangioadenitis (dla). it is not certain whether dla is of filarial or bacterial etiology. the frequency of episodic dla does not depend on the presence or absence of microfilariae. antibiotic therapy is effective in prevention and treatment of dla. these observations point to the bacterial rather than filarial etiology of dla. skin and lymph node biopsies, tissue fluid, lymph, and blood from patients with chronic filarial lymp ...	1997	9242310
a new phospholipase a2 inhibitor, unrelated to substrate analogues: kinetic characterization of the inhibition of secretory phospholipases a2 by pms 832.	starting from a series of compounds which were known to be paf antagonists, we have synthesized molecules that are good inhibitors of pla2s of groups i or ii, with ic50 in the micromolar range (binisti et al., 1997). in this report we investigate the mechanism of inhibition of bovine and porcine pancreatic phospholipases a2 (group i), and platelet lysate phospholipase a2 (group ii) by one of these compounds, 1-(4'-methoxybenzoyl)-2-n-tridecylpiperazine (pms 832). we show that pms 832 behaves as ...	1997	9246606
the effects of agar concentration on the growth and morphology of submerged colonies of motile and non-motile bacteria.	the growth and morphology of submerged bacterial colonies was investigated. five separate colonial forms were recognized depending both on species and on agar concentration. these were (i) branched, dendritic structures seen only with bacillus cereus; (ii) lenticular colonies for all other species at high agar concentrations; (iii) small lobed to spherical colonies for non-motile organisms at low agar concentrations; (iv) and (v) large diffuse spherical colonies which can be further subdivided i ...	1997	9246773
antimicrobial activity of food-related penicillium sp. against pathogenic bacteria in laboratory media and a cheese model system.	food-related penicillium species (n = 34) and geotrichum candidum (n = 11) grown on czapek dox and brie agar were tested for their ability to suppress growth of pathogenic bacteria. ten out of 13 p. camemberti showed antagonistic activity while the other species did not interact significantly with the bacterial growth. the order of inhibition was: gram-negative bacteria and bacillus cereus > listeria monocytogenes, lactococcus sp. > micrococcus sp. whereas lactobacillus sp., staphylococcus aureu ...	1997	9246777
neutralization of hemolytic and mouse lethal activities of c. perfringens alpha-toxin need simultaneous blockade of two epitopes by monoclonal antibodies.	three murine monoclonal antibodies (mabs 3b4, 1e8, 1f9) were produced by fusion of x63-ag8.653 myeloma cells and splenocytes of mice immunized with glutaraldehyde-inactivated alpha-toxin of clostridium perfringens. all mabs belonged to the immunoglobulin g (igg) class and possessed a kappa light chain. all the mabs were specific for alpha-toxin of c. perfringens as demonstrated by immunoblotting experiments performed with culture supernatants of c. perfringens, c. bifermentans, c. sordellii, and ...	1997	9250775
contamination of bacillus cereus in an apheresis-derived platelet concentrate.		1997	9263119
production, purification and characterization of bacillus lipase.	the lipolytic activities in the supernatant fractions of bacillus cereus and bacillus coagulans cultures were investigated. aeration, agitation, different media, emulsified oils, inoculum size and phase of growth affected lipase production. aeration was essential for lipase production (air: medium ration 4:1) and produced the highest activity. the lipolytic activity reached a maximum level after incubation for two days with continuous agitation. it was also elevated by the presence of either oli ...	1997	9265771
aspects of the pharmacokinetics of doxycycline given to healthy and pneumonic east african dwarf goats by intramuscular injection.	the effect of experimentally induced pasteurella haemolytica pneumonia on the pharmacokinetics of doxycycline (doxycen retard) administered intramuscularly was studied in seven east african dwarf goats. the study was conducted in two consecutive phases, separated by a washout period of four weeks. the experimental infection, induced by intratracheal administration of 5 ml of 10(7) to 10(9) cfu/ml of pasteurella haemolytica, produced a temperature rise, depression and laboured breathing within 6- ...	1997	9266665
cloning, overexpression, refolding, and purification of the nonspecific phospholipase c from bacillus cereus.	bacillus cereus secretes a nonspecific phospholipase c (plc) that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. b. cereus plc has been overexpressed with its signal sequence in escherichia coli using a t7 expression system. the expressed enzyme formed intracellular inclusion bodies which were solubilized in the presence of 8 m urea. renaturation was initiated by gradual removal of urea and addition of zinc ions. the signal peptide was specifically c ...	1997	9268684
diversity and differential distribution of is231, is232 and is240 among bacillus cereus, bacillus thuringiensis and bacillus mycoides.	bacillus cereus, bacillus thuringiensis and bacillus mycoides are very closely related bacteria, generally considered as subspecies of b. cereus sensu lato. different transposable elements have been isolated from b. thuringiensis, including is231, is232 and is240 and their variants. the distribution of these three insertion sequences (is) within the b. cereus group has been investigated in 90 strains of b. thuringiensis (representing 61 serovars), in 30 reference strains of b. cereus and in 33 s ...	1997	9274007
comparative sensitivity of 13 species of pathogenic bacteria to seven chemical germicides.	the relative resistance of diverse human bacterial pathogens to commonly used germicidal agents has not been established.	1997	9276546
effect of storage temperatures and ingredients on growth of bacillus cereus in coffee creamers.	growth of bacillus cereus atcc 33018 was evaluated in half and half (10.5% fat), whipping cream (30% fat), and nondairy creamer (7.5% fat). samples were inoculated with approximately 10 vegetative cells/ml or 100 spores/ml and were subsequently stored at 4, 7, 23 and 32 degrees c. within 9 h at 32 degrees c and 11 h at 23 degrees c, in both half and half and whipping cream, vegetative cells and spores reached population levels that can cause foodborne illness. no growth occurred in any product s ...	1997	9276792
substrate binding and catalytic mechanism in phospholipase c from bacillus cereus: a molecular mechanics and molecular dynamics study.	for the first time a consistent catalytic mechanism of phospholipase c from bacillus cereus is reported based on molecular mechanics calculations. we have identified the position of the nucleophilic water molecule, which is directly involved in the hydrolysis of the natural substrate phosphatidylcholine, in phospholipase c. this catalytically essential water molecule, after being activated by an acidic residue (asp55), performs the nucleophilic attack on the phosphorus atom in the substrate, lea ...	1997	9279125
[bactericidal activity determination of biseptine, combination of chlorhexidine, benzalkonium chloride and benzylic alcohol, on 124 hospital bacterial strains].	biseptine is an antiseptic composed of chlorhexidine digluconate (chx), benzalkonium chloride (bc) and benzylic alcohol. minimal bactericidal concentrations (mbcs) of biseptine were determined on 124 clinical strains: 76 enterobacteriaceae, 16 other gram negative bacilli, (pseudomonas spp, aeromonas spp, haemophilus spp) and 32 gram positive bacteria (staphylococcus spp, streptococcus spp, listeria spp, bacillus cereus), using microdilution method, in comparison with hibitane champ. modal mbc of ...	1997	9296082
hdl3 binds to glycosylphosphatidylinositol-anchored proteins to activate signalling pathways.	previous studies have indicated that in hepg2 cells hdl3-signalling involves glycosylphosphatidylinositol (gpi) anchored proteins. hdl3-binding to hepg2 cells was found to be enhanced by cellular preincubation with pi-plc inhibitors and sensitive to a cellular preincubation with exogenous pi-plc, suggesting that hdl3 binds directly on gpi-anchored proteins to initiate signaling. moreover hdl3-binding was found to be partly inhibited by antibodies against the hdl-binding protein (abhbp). hdl3, wh ...	1997	9296527
determination of the kinetic parameters for phospholipase c (bacillus cereus) on different phospholipid substrates using a chromogenic assay based on the quantitation of inorganic phosphate.	the kinetic parameters of the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plcbc) have been evaluated for phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine substrates with a new assay based on the quantitation of inorganic phosphate (pi). treatment of the phosphomonoester product of the plcbc-catalyzed hydrolysis of these phospholipids with alkaline phosphatase releases pi. this pi forms a complex with ammonium molybdate that is then reduced by ascorbi ...	1997	9300081
determination of pka values of the histidine side chains of phosphatidylinositol-specific phospholipase c from bacillus cereus by nmr spectroscopy and site-directed mutagenesis.	two active site histidine residues have been implicated in the catalysis of phosphatidylinositol-specific phospholipase c (pi-plc). in this report, we present the first study of the pka values of histidines of a pi-plc. all six histidines of bacillus cereus pi-plc were studied by 2d nmr spectroscopy and site-directed mutagenesis. the protein was selectively labeled with 13c epsilon 1-histidine. a series of 1h-13c hsqc nmr spectra were acquired over a ph range of 4.0-9.0. five of the six histidin ...	1997	9300493
modification of methane emission in sheep by cysteine and a microbial preparation.	modification of methane emission from ruminants by the use of l-cysteine and/or a microbial preparation in wethers fed lucerne hay was assessed in an open circuit respiratory trial according to a 4 x 4 latin squared design. l-cysteine (0.1 g kg-1 w0.75, as sulphur equivalent) was drenched daily to wethers as an aqueous solution of hydrochloride. the microbial preparation was a dried mixture of bacillus subtilis, bacillus cereus, bacillus thuringiensis, pseudomonas fluorescens, streptomyces cellu ...	1997	9301098
domain evolution in the alpha-amylase family.	the available amino acid sequences of the alpha-amylase family (glycosyl hydrolase family 13) were searched to identify their domain b, a distinct domain that protrudes from the regular catalytic (beta/alpha)8-barrel between the strand beta3 and the helix alpha3. the isolated domain b sequences were inspected visually and also analyzed by hydrophobic cluster analysis (hca) to find common features. sequence analyses and inspection of the few available three-dimensional structures suggest that the ...	1997	9302327
insertional inactivation of a tet(k)/tet(l) like transporter does not eliminate tetracycline resistance in bacillus cereus.	bacillus cereus atcc 10987 and atcc 14579 can be induced to high levels of resistance to tetracycline. the chromosomal b. cereus gene bctl encodes a transmembrane protein with homology to gram-positive tetracycline efflux proteins and relation to other members of the major facilitator superfamily of transport proteins. a mutant strain containing an insertionally inactivated bctl gene did not show impaired tetracycline resistance. no additional altered phenotype was observed in the mutant. accumu ...	1997	9311114
a study of photobactericidal activity in the phenothiazinium series.	the photodynamic antibacterial properties of a closely related series of commercially available phenothiazinium dyes were tested against a range of pathogenic strains of gram-positive (staphylococcus aureus, enterococcus faecalis, bacillus cereus) and gram-negative organisms (escherichia coli, pseudomonas aeruginosa). the photosensitisers were illuminated using a non-laser light source at a fluence of 1.75 mw cm-2 and this resulted in the enhancement of antibacterial activity in liquid culture. ...	1997	9322071
probing the roles of active site residues in phosphatidylinositol-specific phospholipase c from bacillus cereus by site-directed mutagenesis.	the role of amino acid residues located in the active site pocket of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus[heinz, d. w., ryan, m., bullock, t., & griffith, o. h. (1995) embo j. 14, 3855-3863] was investigated by site-directed mutagenesis, kinetics, and crystal structure analysis. twelve residues involved in catalysis and substrate binding (his32, arg69, his82, gly83, lys115, glu117, arg163, trp178, asp180, asp198, tyr200, and asp274) were individually replac ...	1997	9335537
syntheses and biological activities (topoisomerase inhibition and antitumor and antimicrobial properties) of rebeccamycin analogues bearing modified sugar moieties and substituted on the imide nitrogen with a methyl group.	as a part of studies on structure-activity relationships, several potential topoisomerase i inhibitors were prepared. different analogues of the antitumor antibiotic rebeccamycin substituted on the imide nitrogen with a methyl group were synthesized. these compounds bore either the sugar residue of rebeccamycin, with or without the chlorine atoms on the indole moieties, or modified sugar residues (galactopyranosyl, glucopyranosyl, or fucopyranosyl) linked to the aglycone via a beta- or alpha-n-g ...	1997	9341921
inhibition of bacterial respiration by a low-molecular weight lectin, scyllin, from scylla serrata crab hemolymph.	interaction of plant and/or invertebrate lectins with mammalian cells and different microorganisms is well known. in the present study, we have demonstrated that scyllin, a low molecular weight (mw 4000) lectin from the edible crab scylla serrata hemolymph, purified by galnac-sepharon affinity column followed by mono-q ion exchanger in fplc exhibits antimicrobial activity against bacillus cereus and escherichia coli by inhibiting endogenous respiration as well as exogenous glucose oxidation. in ...	1997	9343934
inhibition, reactivation, and determination of metal ions in membrane metalloproteases of bacterial origin using high-performance liquid chromatography coupled on-line with inductively coupled plasma mass spectrometry.	high-performance liquid chromatography coupled on-line with inductively coupled plasma mass spectrometry (hplc-icp-ms) was used for the characterization of metal ions in several metalloproteases of bacterial origin. the different components of the bacterial extracts were separated on a size-exclusion column. the eluent of the hplc system was continuously transported to the icp-ms system for rapid, reproducible, and sensitive analyses of trace elements in the metalloproteases. two different membr ...	1997	9344414
novel selective and non-selective optical detection of microorganisms.	a new instrument, capable of detecting metabolic changes due to microbiological activity, is described. optical changes in growth media are monitored in a semi-fluid zone that separates the liquid medium containing the sample. data demonstrate that common media can be utilized in conjunction with this rapid automated technology. nutrient broth with the ph dye indicator. bromocresol purple was suitable for total counts. selective media containing dyes were utilized to assess the presence or absen ...	1997	9351264
chitinolytic activity of an endophytic strain of bacillus cereus.	bacillus cereus strain 65, previously isolated as an endophyte of sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kda. the enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (glcnac)2 from the non-reducing end of trimeric chitin derivatives. the chitinase exhibited activity over the ph range 4.5-7.5 and was stable between ph 4.0 and 8.5. the enzyme had an isoelectric point of 6.4. application of b. cereus 65 dire ...	1997	9351279
penicillin-binding protein sensitive to cephalexin in sporulation of bacillus cereus.	cephalexin, cefaclor, cefadroxil, and cefotaxime strongly inhibited sporulation of bacillus cereus ts-4 at 1 microgram/ml. cephalexin was most inhibitory on the sporulation of b. cereus when the antibiotic was added at 3 h after induction of sporulation by nutrient downshift technique. examination of 4',6-diamidino-2-phenylindole-stained cells by fluorescence-phase contrast microscopy showed that cephalexin inhibited the formation of asymmetric septum. by using [3h]penicillin, eight penicillin-b ...	1997	9352657
involvement of imp dehydrogenase activity in induction of sporulation of bacillus cereus.	imp dehydrogenase activity of b. cereus increased parallel to cell growth in ye-emm, where b. cereus did not sporulate. when b. cereus was cultured in a modified g medium, a sporulation medium, the activity reached the highest level at 6 hr and decreased thereafter. after induction of sporulation by nutritional shift down in 1/100 g medium, the enzyme activity decreased to about 5% compared with exponentially growing cells at 1 hr of resuspension. the sporulation rate of b. cereus was over 90% i ...	1997	9352663
activity of phosphatidylinositol-specific phospholipase c from bacillus cereus with thiophosphate analogs of dimyristoylphosphatidylinositol.	phosphatidylinositol-specific phospholipase c (pi-plc) was studied with sonicated dispersions of a thiophosphate analog of phosphatidylinositol, 1, 2-dimyristoyloxypropane-3-thiophospho(1d-1-myo-inositol) (d-thio-dmpi). kinetic parameters were derived from the rate as a function of bulk lipid concentration at constant saturating surface concentration of substrate (case i), and as a function of surface concentration of substrate at a constant saturating bulk concentration of lipid (case ii). the ...	1997	9353901
comparison of biological effect of the two different enterotoxin complexes isolated from three different strains of bacillus cereus.	the cytotoxicity of the two different enterotoxin complexes of bacillus cereus was compared after isolation from three different strains. protein components of non-haemolytic enterotoxin (nhe) of 39 kda, 45 kda and 105 kda were isolated from all of the three strains, whilst proteins b, l1 and l2 of haemolysin bl (hbl) were isolated from supernatants of two strains (f837-76 and 1230-88). these proteins were not detected in strain 0075-95. inhibition of protein synthesis in vero cells was used as ...	1997	9353934
bacillus cereus peritonitis in a chronic peritoneal dialysis patient.		1997	9358536
synthesis of some biologically active agents derived from thieno[2,3-d]pyrimidine derivatives.	the key compound 1-amino-8-iminocyclopenta[b]thieno[2,3-d]pyrimidine (5g) was prepared by reaction of 2-amino-3-cyano cyclopenta[b]thiophene (1) with triethyl orthoformate followed by cyclization with hydrazine hydrate in ethanol. refluxing of 1 with triethyl orthoformate in the presence of acetic anhydride gave an unexpected product 2. while reaction with aromatic amines gave the condensation products 4a-c. reaction of 5g with formic acid, other formate derivatives, ethoxymethylenemalononitrile ...	1997	9362089
a xanthanolide with potent antibacterial activity against methicillin-resistant staphylococcus aureus.	this study was conducted to find constituents of an annual herb, xanthium sibiricum patr er widd, with effective antibacterial activity against methicillin-resistant staphylococcus aureus (mrsa). by monitoring antibacterial activity against mrsa strains, it was shown that a sesquiterpene lactone, identified as [3ar-(3a alpha, 7 beta,8a beta)]-3,3a,4,7,8,8a-hexahydro-7- methyl-3-methylene-6-(3-oxo-1-butenyl)-2h-cyclohepta[b]furan-2-one, or xanthatin, isolated from leaves of the herb, had outstand ...	1997	9364417
diacylglycerol partitioning and mixing in detergent micelles: relevance to enzyme kinetics.	for many of the enzymes that utilize or produce diacylglycerols, detergent mixed micelles are often used in assay systems to solubilize the lipophilic substrates or products. the assumption is often made that the diacylglycerol (dag) is solubilized and well mixed throughout the population of micelles during the time course of the assay. in the present work the partitioning and exchange dynamics of diacylglycerols (from dihexanoyl-dag to didecanoyl-dag) in a variety of detergent micelles have bee ...	1997	9366244
produce handling and processing practices.	in the past decade, outbreaks of human illness associated with the consumption of raw vegetables and fruits (or unpasteurized products produced from them) have increased in the united states. changes in agronomic, harvesting, distribution, processing, and consumption patterns and practices have undoubtedly contributed to this increase. pathogens such as listeria monocytogenes, clostridium botulinum, and bacillus cereus are naturally present in some soil, and their presence on fresh produce is no ...	1997	9366597
crystal structure of the phosphatidylinositol-specific phospholipase c from the human pathogen listeria monocytogenes.	the x-ray crystal structure of the phosphatidylinositol-specific phospholipase c (pi-plc) from the human pathogen listeria monocytogenes has been determined both in free form at 2.0 a resolution, and in complex with the competitive inhibitor myo-inositol at 2.6 a resolution. the structure was solved by a combination of molecular replacement using the structure of bacillus cereus pi-plc and single isomorphous replacement. the enzyme consists of a single (beta alpha)8-barrel domain with the active ...	1997	9367761
in-vitro photobactericidal activity of aminoacridines.	the toxicities of several aminoacridines were measured against pathogenic strains of both gram-positive (staphylococcus aureus, enterococcus faecalis, bacillus cereus) and gram-negative (escherichia coli, pseudomonas aeruginosa) organisms. in several cases, illumination at a light dose of 6.3 j/cm2 resulted in considerable decreases in the minimum lethal drug concentrations required, giving up to 50-fold increases in bactericidal activity. derivatives of 9-aminoacridine (aminacrine) exhibited ph ...	1997	9372431
bacterial phospholipase c upregulates matrix metalloproteinase expression by cultured epithelial cells.	phospholipase c (plc) is a putative virulence factor of several pathogenic bacteria. we studied if exogenous plc would perturb epithelial behavior in infected tissues. gelatin and casein zymography of cell culture medium indicated that the broad-spectrum plc of bacillus cereus induced matrix metalloproteinase (mmp) production in epithelial cells of human skin (nhek), human gingiva (hge), and porcine periodontal ligament (ple). in all three cell types, the strongest increase (ninefold) at 0.1 u/m ...	1997	9393778
bacillus cereus peritonitis in a patient being treated with continuous ambulatory peritoneal dialysis.		1997	9394346
inhibitory effect on curcumin on mammalian phospholipase d activity.	curcumin, the major yellow pigment of turmeric (curcuma longa), has strong anti-carcinogenic and anti-inflammatory activities. we examined the effects of curcumin on enzyme activities of the following phospholipases in a cell-free system: g protein-mediated phospholipase d (pld), phosphatidylinositol-specific phospholipase c, and phospholipase a2 from mouse macrophage-like cell line j774.1 cells, sphingomyelinase from bovine brain, and phosphatidylcholine-phospholipase c from bacillus cereus. cu ...	1997	9395294
diarrhoeal toxin production at low temperature by selected strains of bacillus cereus.	the growth of four bacillus cereus strains producing diarrhoeal toxin at 32 degrees c (f4433/73 and 29.155, isolated on the occasion of foodborne outbreaks, and f4581/76l and f4581/76r, two variants of a clinical strain), a weakly toxigenic strain isolated in routine analysis of food (3505m) and an emetic isolate (f3502/73) was investigated at low temperature. biomass was determined by protein assay. generation times were: for strain f3502/73, which grew at > or = 12 degrees c, 8.71 h (at 12 deg ...	1997	9403769
expression of cereolysine ab genes in bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection.	the cereolysin ab genes from bacillus cereus vkm-b164 have been expressed in bacillus anthracis strains: virulent h-7 (pxo1, pxo2), vaccine sti-1 (pxo1), 221 (without its own plasmids). expression was achieved by cloning the genes in a high copy number plasmid pe194. this construct was integrated with host genomes in amplified form. gold hamsters were vaccinated with parental and recombinant b. anthracis sti-1 and 221 strains and challenged with virulent ones subcutaneously. gold hamsters vaccin ...	1997	9413092
effects of sporulation ph on the heat resistance and the sporulation of bacillus cereus.	spores of bacillus cereus atcc 7004, 4342 and 9818 were obtained in nutrient agar at several ph from 5.9 to 8.3. the optimum ph for sporulation was around 7, but good production of spores was obtained in the range 6.5-8.3. with all three strains, d-values clearly dropped with sporulation ph, decreasing by about 65% per ph unit. z-values were not significantly modified (p > 0.05) by this factor. mean z-values of 7.13 degrees c +/- 0.16 for strain 7004, 7.67 degrees c +/- 0.04 for 4342 and 8.80 de ...	1997	9418067
single strand conformation polymorphism analysis of pcr-tdna fingerprinting to address the identification of bacillus species.	the suitability of tdna-pcr fingerprinting to identify species of the genus bacillus was tested on 75 strains. strains belonging to the same species or the same phylogenetic cluster were correctly grouped. among b. stearothermophilus strains, different pattern types were found. this could be due to the unclear taxonomic situation of these strains, rather than to a failure of the tdna-pcr. single strand conformation polymorphism (sscp) of the pcr products allowed species discrimination within the ...	1997	9418243
inhibition of metallo-beta-lactamases by a series of thiol ester derivatives of mercaptophenylacetic acid.	a series of mercaptophenylacetic acid thiol esters bearing a phenyl substituent adjacent to the carboxylic acid function has been shown to be inhibitors of metallo-beta-lactamases. the inhibition of the bacteroides fragilis cfia and bacillus cereus ii metallo-beta-lactamases was zn2- dependent, greater inhibition being observed at 1 microm znso4 than at 100 microm znso4. despite this zn2+ dependency, isothermal titration calorimetry studies illustrated that representative compounds had no detect ...	1997	9418252
[staphylococcal sepsis associated with platelet transfusion: report of a new case and review of the literature].	bacterial contamination of blood components is an infrequent although potentially fatal event. bacterial contamination rate of platelet concentrates (pc) varies between 1/900 to 1/2,000 random donor units. however the risk of symptomatic bacteriemias is lower (between 1/2,000 to 1/12,000 random donor units). the organisms most commonly implicated in contaminated pc are gram-positive bacteria such as coagulase negative staphylococci, bacillus cereus or propionibacterium acnes, although gram-negat ...	1997	9424743