Publications

structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each.	a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ...	1992	1347504
structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each.	a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ...	1992	1347504
chlamydia trachomatis infection in children with wheezing simulating asthma.		1992	1349398
use of nonoxynol-9 and reduction in rate of gonococcal and chlamydial cervical infections.	the spermicide nonoxynol-9 (n-9) has been used as a contraceptive for over 30 years, but the use of a vaginal spermicide and condoms for the prevention of sexually transmitted infections has not been examined in randomised studies. we report a single-blind randomised field trial to assess the effect of n-9 film on the rate of gonococcal and chlamydial cervical infection in women at high risk of these diseases. 343 women were randomly assigned to use either condoms and n-9 (186 women) or condoms ...	1992	1350804
false-positive immunoassay for chlamydia in urine.		1992	1350844
effects on ingestive behavior in rats of the alpha 1-adrenoceptor agonist cirazoline.	microinjections of various alpha 1-adrenoceptor agonists including phenylephrine and phenylpropanolamine into the paravenricular hypothalamic nucleus (pvn) suppress food intake in rats, suggesting that this receptor type might act in opposition to previously identified facilatory pvn alpha 2-adrenoceptors in the modulation of feeding. in the present experiments, we examine the effects on food and water intake of intra-pvn as well as systemic injection of cirazoline, a highly potent alpha 1-adren ...	1992	1350985
vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia.	the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ...	1992	1351005
intraepithelial gamma delta t-cell-receptor lymphocytes and genetic susceptibility to coeliac disease.	although the proportion of gamma delta t-cell-receptor (tcr)-bearing intraepithelial lymphocytes is increased in the jejunum of patients with active coeliac disease, an abnormality thought to be specific among those with gluten-sensitive enteropathy, the factors influencing gamma delta tcr expression remain uncertain. we examined the relation between genetic factors associated with coeliac disease and intraepithelial gamma delta t lymphocyte distribution in both coeliac patients and their health ...	2005	1351185
intracellular recordings from neurobiotin-labeled cells in brain slices of the rat medial nucleus of the trapezoid body.	principal cells in the medial nucleus of the trapezoid body (mntb) are believed to be critical components in the circuit subserving sound localization. these cells, located in the superior olivary complex, convert excitatory inputs, arriving from the contralateral cochlear nucleus by way of large somatic synapses (the calyces of held), to inhibitory projections onto principal cells in the ipsilateral lateral superior olive (lso). we have characterized a population of cells in the rat mntb using ...	1992	1351938
critical research directions in pediatric hiv infection. keystone symposia for molecular and cellular biology. march 27-31, 1992. abstracts.		1992	1351959
detection of chlamydia trachomatis dna in joints of reactive arthritis patients by polymerase chain reaction.	in 1986, chlamydia trachomatis elementary bodies were found by direct immunofluorescence (dif) in synovial-fluid cell deposits and synovial-membrane biopsy samples from five of eight patients with sexually acquired reactive arthritis (sara) but in none of eight controls with other types of arthritis. cells from the original slides (stored at 4 degrees c) have now been examined by a polymerase chain reaction (pcr) that amplifies dna for the major outer membrane protein of c trachomatis. chlamydia ...	1992	1352016
the possible role of chlamydia trachomatis in perineal suppurative hidradenitis.	to find out if there is an association between perineal suppurative hidradenitis and chlamydia trachomatis infection.	1992	1352134
the influence of vitamin k3 treatment on the pharmacokinetics and metabolism of (+)-propranolol in the rat.	the effects of vitamin k3 treatment on the pharmacokinetics and metabolism of (+)-propranolol and the consequences of hepatic injury associated with vitamin k3 treatment were examined in groups of male sprague-dawley rats. when vitamin k3 (20 mg/kg) in polyethylene glycol 300 (peg 300) was coinfused with (+)-propranolol (2 mg/kg) into the pyloric vein (a tributary flowing directly into the hepatic portal vein), a significant decrease in the intrinsic clearance of total drug (clint) from 94.1 +/- ...	1992	1352223
chaperonins groel and groes promote assembly of heterotetramers (alpha 2 beta 2) of mammalian mitochondrial branched-chain alpha-keto acid decarboxylase in escherichia coli.	we have investigated the possible role of chaperonins groel and groes in the folding and assembly of heterotetramers (alpha 2 beta 2) of mammalian mitochondrial branched-chain alpha-keto acid decarboxylase (e1) in escherichia coli. the mature e1 alpha subunit fused to maltose-binding protein (mbp) was coexpressed with mature e1 beta on the same vector in es- and el- mutant strains. only small or trace amounts of active e1 component were obtained. cotransformation of the es- mutant host with a se ...	1992	1352285
chlamydia trachomatis and wheezing.		1992	1352610
[molecular epidemiology of chlamydia trachomatis by dna restriction endonuclease analysis].		1992	1357211
biological properties and genetic analysis of the ompa locus in chlamydiae isolated from swine.	eight strains of chlamydia psittaci isolated from swine with pneumonia, pleuritis, pericarditis, and enteritis were characterized through analysis of the major outer membrane protein gene ompa by a two-step polymerase chain reaction, by their interactions with cells in culture, and by the morphologic features and ultrastructure of intracellular inclusions. amplified chlamydial ompa dna fragments were differentiated by restriction endonuclease digestion. chlamydial isolates were separated into 2 ...	1992	1358014
immunotherapy for drug-resistant tuberculosis.		1992	1360082
s-100 beta and insulin-like growth factor-ii differentially regulate growth of developing serotonin and dopamine neurons in vitro.	to study the phenotypic specificity of s-100 beta and insulin-like growth factor ii (igf-ii) for developing monoamine neurons, serotonin (5-ht) neurons from the embryonic day 14 (e14) rostral raphe or dopamine (th) neurons from the substantia nigra/ventral tegmental area were cultured for 3 days in vitro (3 div) in the presence of these factors. neuronotrophic effects were analyzed by computer-assisted morphometry of 5-ht and th-immunoreactive neurons. s-100 beta and igf-ii differentially regula ...	1992	1360544
pcr detection and differentiation of chlamydia pneumoniae, chlamydia psittaci and chlamydia trachomatis.	a pcr-based system was developed for the detection and differentiation of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae. a conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. the three species were then differentiated from each other by digestion of this pcr product with restriction enzymes eco ri and either hind iii or pst i. the system was shown to work for two strains of c. pneumoniae, 11 strains of c. psittaci and 10 serovars of c. ...	1992	1361961
distinguishing chlamydia species by restriction analysis of the major outer membrane protein gene.	clinical isolates of chlamydia pneumoniae from diverse geographic locations and strains of other chlamydia species were typed by polymerase chain reaction (pcr) amplification of the major outer membrane protein (momp) gene followed by restriction fragment length polymorphism analysis of the product. use of synthetic primers corresponding to highly conserved regions of the momp gene resulted in amplification of a 1070 bp product in laboratory strains and clinical isolates of c. pneumoniae, c. tra ...	1992	1361962
gene typing of chlamydia trachomatis by polymerase chain reaction and restriction endonuclease digestion.	a portion of the major outer membrane protein (momp) gene from 15 chlamydia trachomatis serovars was amplified by polymerase chain reaction (pcr) and the product was analyzed by restriction fragment length polymorphism (rflp). a set of primers was used to amplify an 871 base pair gene fragment encompassing the 4 hypervariable regions of momp. alui digestion of the product gave distinctive patterns for the 15 serovars as demonstrated on silver-stained polyacrylamide gels. a triple digest with eco ...	1992	1362824
brain serotoninergic stimulation reduces the water intake induced by systemic and central beta-adrenergic administration.	1. this study was undertaken to determine if the stimulation of central serotoninergic receptors affects the thirst-inducing action of systemically or intracerebroventricularly (icv) injected isoproterenol. 2. male wistar rats weighing 220-300 g were used in groups of 10-14 animals each. normally hydrated rats implanted with a delay cannula into the third ventricle were injected icv with the 5ht1c/5ht2 agonist mk212 (50 nmol/2 microliters) prior to administration of isoproterenol sc (330 microgr ...	1992	1364146
histiocytosis x with thyroid involvement: report of a case.	histiocytosis x is a disease of histiocyte proliferation in response to some unknown etiology. thyroid involvement is extremely rare in the literature. in this paper, we present an 18-year-old female with histiocytosis x with thyroid involvement. this patient had had a goiter with normal thyroid function since 12 years of age. a thyroidectomy was done under the suspicion of thyroid cancer. pathology revealed histiocytosis x. hypoparathyroidism and hypothyroidism were noted after the operation an ...	1998	1364223
chlamydial infection in canada.		1992	1365519
[various therapeutic protocols used in genital disease caused by chlamydia trachomatis].		1991	1365577
[action of ++clarithromycin against mycoplasma and chlamydia].		1991	1365578
[pelvic inflammatory disease and its treatment].		1992	1366085
an enhanced-response system for performing chemiluminescent and bioluminescent tests.		2006	1366493
brave new biosensors.		2006	1366899
cell growth and enzyme synthesis of a mutant of arthrobacter sp. (dsm 3747) used for the production of l-amino acids from d,l-5-monosubstituted hydantoins.	a microorganism with the ability to form l-tryptophan from d,l-5-(3-indolyl-methyl)hydantoin (d,l-5-imh) was isolated and identified as arthrobacter sp. (dsm 3747). after isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high amounts of biomass with good specific activities concerning the enzymatic hydantoin-cleaving reactions. the ability of the microorganism to perform these bioconversions was found to b ...	1990	1366910
unknown sequence amplification: application to in vitro genome walking in chlamydia trachomatis l2.	a recently described technique, 'chemical genetics' unknown sequence amplification method, which requires only one specific oligonucleotide, has broadened the applicability of the polymerase chain reaction to dna of unknown sequence. we have adapted this technique to the study of the genome of chlamydia trachomatis, an obligate intracellular bacterium, and describe modifications that significantly improve the utility of this approach. these techniques allow for rapid genomic analysis entirely in ...	1991	1367216
characteristics of a glycoprotein in the ocular surface glycocalyx.	a monoclonal antibody has been produced that binds to the apical squames (flattened cells) of the rat ocular surface epithelium and to the goblet cells of the conjunctiva. immunoelectron microscopic localization of the antigen indicates that in apical cells it is present along the apical-microplical membrane in the region of the glycocalyx. in subapical squames, the antigen is in cytoplasmic vesicles. in some goblet cells, the antigen is in the golgi network, and in others, it is located primari ...	1992	1370440
immunogenicity of a chimeric peptide corresponding to t helper and b cell epitopes of the chlamydia trachomatis major outer membrane protein.	the immunogenicity of a chimeric t/b cell peptide corresponding to antigenically characterized epitopes of the chlamydia trachomatis major outer membrane protein (momp) was studied in mice to further define its potential use in the development of a subunit vaccine in preventing blinding trachoma in humans. the chimeric peptide, designated a8-vdi, corresponds to a conserved momp t helper (th) cell epitope(s) (a8, residues 106-130) and serovar a vdi (residues 66-80), which contains the serovar-spe ...	1992	1370528
termination of second messenger signaling in olfaction.	by using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ...	1992	1370581
regulation of annexin i in adipogenesis: camp-independent action of methylisobutylxanthine.	the differentiation of 3t3-l1 fibroblasts to adipocytes can be accelerated by the addition of 1-methyl-3-isobutylxanthine (mix), insulin, and dexamethasone to the culture medium. during differentiation, we have demonstrated that the level of both annexin i mrna and protein decreases. the half-times for this reduction were 2 h and 10 h for annexin i mrna and protein, respectively. of the added agents in the differentiation medium, only mix caused a decline in annexin i expression in 3t3-l1 fibrob ...	2005	1370745
biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis.	the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ...	1992	1370931
the existence of two completely distinct antigenic sites within a decapeptide.	a decapeptide (1182-1191) derived from the bovine interphotoreceptor retinoid-binding protein (irbp) was found to contain two completely distinct antigenic sites when tested in lewis rats. one site, localized in sequence 1182-1191, is the core of the immunodominant and highly uveitogenic determinant of irbp. the second site localizes within sequence 1183-1191 and becomes detectable only when tryptophan at position 1182 is deleted. lymphocytes sensitized against the first, larger site recognized ...	1992	1371240
isolation and characterization of a human gene that encodes a new subclass of protein tyrosine kinases.	we describe the isolation and cdna sequence of a novel human gene, which is distinct from all known members of the human src family of proto-oncogenes. in contrast to these, an autophosphorylation site corresponding to tyr416, as well as the equivalent of tyr527 in p60c-src, are missing in the amino acid (aa) sequence deduced from this gene. furthermore, no n-terminal myristylation site is found. our human clone is 98% identical at the aa level to a gene which was isolated independently from neo ...	1992	1371489
prevention of post-ischemic brain lipid conjugated diene production and neurological injury by hydroxyethyl starch-conjugated deferoxamine.	hydroxyethyl starch conjugated deferoxamine (dfo) was administered to rats following resuscitation from 6.5 min cardiac arrest (ca) in an attempt to prevent the iron-catalyzed production of oxygen free radicals which may lead to neurologic injury and ultimately death following restoration of spontaneous circulation (rosc). brain conjugated dienes were analyzed spectrophotometrically 4 and 24 hr following rosc, and were found to be significantly elevated when compared to non-ischemic controls. hy ...	1992	1371490
comparison of cyclosporine and fk506 effects on glutathione levels in rat cochlea, brain, liver and kidney.	cyclosporine treatment (50 mg/kg/day, p.o.) caused increases in rat renal reduced glutathione (gsh) levels of 205 and 673%, respectively, after 5 and 10 days. no changes were seen in liver gsh with either dose of cyclosporine. fk506 (2.5 mg/kg/day, p.o., for 7 days) caused an approximately 200% increase in kidney gsh, and an approximately 250% increase in hepatic gsh levels. oxidized glutathione (gssg) was never more than 1-2% of the level of the reduced form in any tissue from control animals. ...	1992	1371687
characteristics of three vaginal flora patterns assessed by gram stain among pregnant women. vaginal infections and prematurity study group.	this study was undertaken to define the characteristics and persistence of vaginal flora in 7918 pregnant women at 23 to 26 weeks' gestation. vaginal smears were categorized as normal (predominant lactobacilli), intermediate (reduced lactobacilli), or positive for bacterial vaginosis. the women with normal flora were least likely to have elevated vaginal ph, amine odor, milky discharge, or colonization by gardnerella, bacteroides, or genital mycoplasmas. women with intermediate vaginal flora had ...	1992	1372474
chlamydial rrna in the joints of patients with chlamydia-induced arthritis and undifferentiated arthritis.	synovial fluid and synovial membrane specimens of 11 patients with chlamydia-induced arthritis (cia), 24 patients with undifferentiated arthritis (unda), 4 patients with post-enteritic reactive arthritis, 3 patients with lyme arthritis and 9 patients with rheumatoid arthritis were investigated for the presence of chlamydia trachomatis (c. trachomatis). a single stranded dna-probe was used for nucleic acid hybridization with ribosomal rna (rrna) from c. trachomatis. in 4 patients (cia = 1, unda = ...	1992	1372544
demonstration of chlamydial rna and dna during a culture-negative state.	trachoma is a common blinding disease of humans caused by ocular infections with chlamydia trachomatis. the cynomolgus monkey is a valuable primate model for the detection, pathobiology, and treatment of this infection. we have used this model system to compare the relative ability of tissue culture, direct fluorescence cytology, a modified polymerase chain reaction, and rna blotting to detect c. trachomatis following primary infection and reinfection over 34 weeks. six cynomolgus monkeys were g ...	1992	1373404
identification of chlamydia pneumoniae by dna amplification of the 16s rrna gene.	chlamydia pneumoniae is an important cause of respiratory disease in humans, but diagnosis of c. pneumoniae is hindered by difficulties in the in vitro growth of the organism. in order to improve detection and identification, we recently developed a polymerase chain reaction (pcr) assay which uses oligonucleotide primers specific for c. pneumoniae. the nucleic acid sequence was determined for the 16s rrna of c. pneumoniae, and regions in which c. pneumoniae differed from both chlamydia psittaci ...	1992	1374077
inapparent ocular infection by chlamydia trachomatis in experimental and human trachoma.	there is substantial indirect evidence which suggests that chlamydia trachomatis can generate inapparent, persistent infections in human. to confirm this directly, we examined ocular chlamydial infection in both the cynomolgus monkey model of trachoma and in patient samples from a trachoma-endemic area. in monkeys, ocular infection was studied over time using direct immunofluorescence cytology (dfa) and a molecular hybridization screening system which targets chlamydial ribosomal rna. in eleven ...	1992	1375138
monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60.	ocular and urogenital tract infections with chlamydia trachomatis can progress to chronic inflammatory diseases that produce blindness and tubal infertility. the pathophysiology of these chronic disease conditions is thought to be immunologically mediated, and the chlamydial 60-kda heat shock protein (hsp60) has been implicated as a major target antigen that stimulates the immunopathological response. the lack of chlamydial hsp60 antibodies and purified hsp60 has severely restricted studies to d ...	1992	1375196
unusual prevalence of the rare serovar da of chlamydia trachomatis in greece detected by monoclonal antibodies.	twenty-seven monoclonal antibodies (mab), eight with subclass-specificities and nineteen reacting with one or two c. trachomatis serovars, were developed and used to immunotype twenty-six clinical isolates from greek patients with chlamydial infections. twelve samples, first classified as serovar d, were identified as the recently established serovar da on the basis of their negative reaction with mab jg9, an mab previously shown to distinguish between d and da, and a new mab 114d9 with similar ...	1992	1375784
detection and antigenicity of chlamydial proteins that bind eukaryotic cell membrane proteins.	chlamydia psittaci proteins capable of binding eukaryotic cell membranes were identified and antigenically characterized. cell membrane proteins (cmp) of noninfected cells were labeled with biotin (b-cmp), then were extracted with 1% triton x-100. nitrocellulose membrane strips containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis-resolved proteins of chlamydial elementary bodies (eb) were reacted with the b-cmp extract, followed by addition of streptavidin-conjugated horse radish ...	1992	1378251
identification and characterization of a calmodulin-dependent nitric oxide synthase from gh3 pituitary cells.	a nitric oxide synthase activity stimulated more than 30-fold by the concurrent presence of ca2+ and calmodulin (cam), and inhibited by trifluoperazine (50 microm), has been identified in extracts of gh3 pituitary cells. the cam-dependent nitric oxide synthase of the crude extract was stimulated more than 9-fold by (6r)-5,6,7,8-tetrahydro-l-biopterin with half-maximal stimulation occurring at a concentration of 300 nm. fractionation of the extract on deae-cellulose enhanced nitric oxide synthase ...	1992	1379040
clarithromycin. a review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential.	clarithromycin is an acid-stable orally administered macrolide antimicrobial drug, structurally related to erythromycin. it has a broad spectrum of antimicrobial activity, similar to that of erythromycin and inhibits a range of gram-positive and gram-negative organisms, atypical pathogens and some anaerobes. significantly, clarithromycin demonstrates greater in vitro activity than erythromycin against certain pathogens including bacteroides melaninogenicus, chlamydia pneumoniae, chlamydia tracho ...	1992	1379907
vasoactive peptides and growth factors in the pathophysiology of hypertension.	a hallmark of vascular disease is the inappropriate proliferative and synthetic behavior of smooth muscle cells. this phenotypically immature behavior arises as a consequence of the myocytes undergoing conversion from a contractile to proliferative/secretory cell type. the stimulus invoked for this dedifferentiative process has been, until recently, endothelial desquamation and subsequent acute exposure of the smooth muscle cells to platelet-derived mitogens. pathogenic conversion of myocytes oc ...	1996	1380620
identification of a repetitive element in the snail biomphalaria glabrata: relationship to the reverse transcriptase-encoding sequence in line-1 transposons.	bamhi-digested biomphalaria glabrata dna contains a repetitive 2.0-kb fragment which is readily discernible by ethidium bromide staining. we present evidence that this repetitive element is related at both the nucleotide and amino acid levels to long interspersed nuclear element (line)-like transposons. although comparable elements have been described in several invertebrates, this is the first report of a molluscan homologue. in common with line transposons, an open reading frame in the b. glab ...	1997	1380940
fk-506-binding proteins from streptomycetes producing immunosuppressive macrolactones of the fk-506 type.	fk-506-binding proteins (fkbps), which in t cells are supposed to mediate the immunosuppressive effects of the compounds fk-506 and rapamycin, have been isolated from streptomyces chrysomallus, s. hygroscopicus subsp. ascomyceticus, and s. hygroscopicus. the latter two strains are producers of ascomycin (the ethyl analog of fk-506) and rapamycin, respectively. like the 12-kda fkbp in eukaryotic organisms such as humans, bovines, and saccharomyces cerevisiae, or the fkbps from gram-positive strep ...	1992	1381710
insulin-like growth factor binding protein (igfbp-3), an inhibitor of serum growth factors other than igf-i and -ii.	our results show that an insulin-like growth factor binding protein, igfbp-3, purified from rat serum, is an inhibitor of chick embryo fibroblast (cef) growth. it abolished dna synthesis in cef stimulated by igf-i as well as by human serum. rat igfbp-3 and idf45 (an inhibitory diffusible factor secreted by mouse cells) had the same activities, confirming that they have an intrinsic capacity to inhibit serum stimulation and may be considered as growth inhibitors. our data show that inhibition by ...	1992	1381713
effective treatment of urethritis. a practical guide.	most cases of urethritis can be readily treated using recommended regimens. the most important causes of urethritis are chlamydia trachomatis and neisseria gonorrhoeae, and initial treatment is directed at them. optimal management requires obtaining a thorough sexual history, evaluation for objective clinical and laboratory evidence of infection, antimicrobial therapy directed towards the major aetiologies, and evaluation and treatment of sexual partners. treatment of gonorrhoea requires a singl ...	1992	1382015
arg276 of gsea, a chlamydia trachomatis kdo transferase, is required for the synthesis of the chlamydial genus-specific epitope in escherichia coli.	gsea is an enzyme from chlamydia trachomatis that can catalyse the addition of three 3-deoxy-d-manno-octulosonic acid (kdo) residues onto lipid a precursors. gsea is similar, and in a few stretches identical, in its amino acid sequence to kdta, an escherichia coli kdo transferase. in this study we altered an amino acid of gsea in a region that is identical between gsea and kdta to test its importance in the structure or catalytic activity of gsea. we found that when arg276 was changed to lys, il ...	1992	1382035
a novel 3-deoxy-d-manno-octulosonic acid transferase from chlamydia trachomatis required for expression of the genus-specific epitope.	dna cloned from chlamydia trachomatis is able to direct the formation of the genus-specific lipopolysaccharide epitope of chlamydiae in enteric gram-negative bacteria. we now demonstrate that a single c. trachomatis gene (gsea) is sufficient to impart this trait to escherichia coli. the deduced amino acid sequence of gsea shows 23% identity (66% similarity) to kdta, an e. coli gene that codes for a bifunctional enzyme catalyzing the addition of two 3-deoxy-d-manno-octulosonic acid (kdo) residues ...	1992	1382060
the role of lipopolysaccharide in the exposure of protective antigenic sites on the major outer membrane protein of chlamydia trachomatis.	a species-specific monoclonal igm antibody (mab) 9bf8 directed against the major outer membrane protein (momp) of chlamydia trachomatis neutralized several chlamydial serovars in a complement-independent manner. the presence of mg2+ ions negated the neutralization in serovars f, l1 and l2, but not in serovars a, b, e, d and k. the ability of monovalent fab-fragments of this mab to neutralize chlamydial infectivity in a mg-independent manner suggested that conformational alterations on the chlamy ...	1992	1382112
[development of serodiagnostic kit "hitazyme chlamydia ab" for chlamydia trachomatis infections using extracted antigen].	to develop eia kit with low cross-reactivity for the quantitative detection of anti-chlamydial antibodies, we examined the preparation of trachomatis antigens and its specificity to mouse antisera and human sera. the chlamydial elementary body (eb) purified from c. trachomatis l2/434/bu strain was treated by sarkosyl, dithiothreitol and sds by turns to obtain the soluble eb outer membrane (comc). sds-page showed that the major components of the comc were 96k, 60k and 39.5 kda peptides. the react ...	1992	1383356
specificity of a tumor marker (ca54/61) and its individual epitopes recognized by monoclonal antibodies, ma54 and ma61, in human tumor patients.	the specificity of a tumor marker (ca54/61) and its individual epitopes (ca54 and ca61) recognized by monoclonal antibodies (ma54 and ma61) and expressed by the same tumor marker were studied. serum levels of ca54 and ca61 were compared with that of ca54/61. in lung adenocarcinoma and ovarian carcinoma, the positive rates of ca61 (42% and 68%) were higher than those of ca54 (32% and 32%) and similar to those of ca54/61 (45% and 74%). the serum levels of ca54 and ca61 showed a significant correla ...	1992	1383482
estimating trauma centre workload.		2006	1383533
effects of chronotropic responsive cardiac pacing on ventilatory response to exercise in patients with complete av block.	to identify the effect of chronotropic responsive cardiac pacing on the ventilatory response to exercise, ten selected patients with complete atrioventricular block underwent paired cardiopulmonary exercise tests in fixed rate ventricular (vvi) and dual chamber (ddd) or rate responsive ventricular (vvir) pacing modes. compared to vvi pacing, ddd or vvir pacing increased peak oxygen uptake (p < 0.005) and augmented anaerobic threshold (p < 0.001). in eight patients, dyspnea was the major symptom ...	1996	1383960
endomyocardial biopsy approach in cases with ventricular arrhythmias.	right ventricular endomyocardial biopsies were performed in patients with ventricular tachycardia (vt: n = 20) and idiopathic premature ventricular contraction (pvc: n = 23). active myocarditis was found in one case and postmyocarditic change in 9 cases. significant pathology according to our definition was found in 9 out of the total of 43 cases (20.5%). the pathological features of the 9 cases were interstitial fibrosis in 5, disarrangement of muscle bundles in 6, degeneration of myocytes in o ...	1995	1384018
seroepidemiologic study of hepatitis c virus in sexually transmitted disease risk groups.	to identify the importance of heterosexual activity as a possible route for the transmission of the hepatitis c virus (hcv), a screening of antibodies against hcv (anti-hcv) was performed in 200 sexually transmitted disease patients with different risks for incurring genital infections as well as in 100 registered prostitutes. out of all 300 persons tested, 14 cases of hcv infection were detected. anti-hcv was present in 3 of the prostitutes and in 11 of the std patients. evaluating known risk f ...	1992	1384151
cross-reactive antigen recognition by an encephalitogenic t cell receptor. implications for t cell biology and autoimmunity.	the dominant immune response to rat myelin basic protein in h-2u mice is directed against the acetylated, n-terminal peptide ac1-11 (acasqkr-psqrhg). this peptide causes encephalomyelitis on injection into mice of the h-2u haplotype. only two residues of the peptide are required for ligation of the tcr from an ac1-11-specific t cell hybridoma. proline at position 6 could not be substituted by any other l-amino acid, whereas glutamine at position 3 could be replaced by phenylalanine, histidine, m ...	1992	1385532
[effects of iodine-131 in sheep depending on the content of stable iodine in the diet].	the biological effect of 131i was studied in sheep kept on a diet deficient in stable iodine. an increased capture and accretion of iodine in the thyroid gland and in the whole body were observed. the disturbances in the structure and function of the thyroid gland, liver and haemopoietic organs were more pronounced in the animals kept on the iodine deficient diet.	1992	1386676
carbogen and nicotinamide: expectations too high? (response to j. martin brown)		2006	1386677
[allergic drug reactions in children].	we present a retrospective study of allergic drug reactions seen in our pediatric allergy consulting room during the last 6 years. during this time, 840 patients were examined for suspected adverse drug reactions. drug allergy was confirmed in 72 cases (8.5%). of these cases, 29 (40.2%) were considered to be ige mediated, or immediate hypersensitivity reactions. we have not found significant differences with regards to age, sex, atopic family history or atopia between patients with ige mediated ...	2006	1386717
[asymptomatic carriage of chlamydia trachomatis in men consulting for condylomata acuminata].	a search for chlamydia trachomatis by cell culture was carried out in the urethra of 82 male patients consulting for condyloma acuminata at the clinical and biological centre for sexually transmissible diseases of the saint-louis hospital, paris. three patients had discreet urethral signs, but none had urethral discharge. cell culture was positive for c. trachomatis in 36 of the 82 patients (44 percent). this high prevalence suggests that c. trachomatis should systematically be looked for in the ...	1992	1387948
aromatization inhibition alone or in combination with gnrh agonists for the treatment of premenopausal breast cancer patients.	aromatase inhibition in postmenopausal women causes a marked fall in the plasma levels of oestrogens and is an effective treatment for breast cancer, however, trials with aminoglutethimide found that this aromatase inhibitor was ineffective in suppressing plasma oestrogen levels in premenopausal breast cancer patients. we found that the more potent inhibitor, 4-hydroxyandrostenedione (4-oha), which can suppress oestrogen synthesis in rodents and non-human primates with intact ovarian function, w ...	1992	1388047
the cancer-associated retinopathy antigen is a recoverin-like protein.	cancer-associated retinopathy (car) is a rare form of retinal degeneration that occurs in association with certain forms of cancer. car patients typically possess high titers of autoantibodies against a specific photoreceptor protein--the 23 kd retinal car antigen. the mechanisms involved in the vision loss experienced by car patients are not understood, but serologic studies indicate the process could include a series of autoimmune reactions directed at specific components of the retina. becaus ...	1992	1388144
reconstitution of long-term t helper cell function after zidovudine therapy in human immunodeficiency virus-infected patients.	peripheral blood mononuclear cells from 12 asymptomatic patients infected with immunodeficiency virus (hiv) and 4 patients with aids were analyzed before and during therapy with zidovudine for t helper cell (th) function. th function improved by more than fourfold to one or more of three stimuli tested in 9 (75%) of 12 asymptomatic patients on zidovudine therapy and in 3 of 4 patients with aids. only 6 (7.4%) of 80 untreated hiv-infected control patients showed spontaneous improvement in th func ...	1992	1388195
[ecologic significance of m. avium infectiousness of waterside and aquatic birds].	a total of 777 specimens of the river-side and water birds of 36 species belonging to 8 orders were examined for infectivity by the causative agent of tuberculosis m. avium. examination was carried out in the northern zone of the land along the lower volga. infectivity was studied by serologic and bacteriologic tests. serologic examination showed that 104 out of the 399 birds were ait-positive (26.0 +/- 2.195%), in bacteriologic examination 73 out of the 378 specimens were infected (19.57 +/- 2. ...	1992	1388267
eukaryotic cells grown on microcarrier beads offer a cost-efficient way to propagate chlamydia trachomatis.	the use of microcarrier cell culture as a method for the in vitro propagation of the obligate intracellular bacterial parasite, chlamydia trachomatis, is described. the microcarrier beads proved to be a more cost-effective means to propagate c. trachomatis than traditional tissue culture flasks or roller bottles without sacrificing yields or infectivity. in addition, microcarrier cell culture was found to be a much simpler technique to study the intracellular development of these bacteria.	1992	1389171
comparison of the self-image of teenagers in finland, the united states, and germany.	in this research, the self-image of finnish adolescents was compared with the self-images of american and west german adolescents. results show that there are statistically significant differences between finnish adolescents and american adolescents in five areas. finnish adolescents are emotionally healthier than american adolescents, and their mood is better. american adolescents have higher vocational and educational goals than finnish adolescents, and their adaptation skills to immediate env ...	2006	1390794
chlamydia trachomatis antigen detection by chlamydiazyme combined with chlamydia blocking reagent verification.	several options exist for the detection of chlamydial infection in a routine laboratory setting. enzyme immuno assay (eia) technology offers rapid turn around of results and is less technically demanding than chlamydial cell culture. in addition, recently introduced eia confirmatory reagents have the potential to improve the accuracy of eia detection. we have evaluated one such confirmatory reagent (chlamydia blocking reagent, abbott laboratories) to determine the accuracy of the chlamydiazyme e ...	1992	1391063
pelvic inflammatory disease.	pelvic inflammatory disease is a common serious complication of the sexually transmitted pathogens neisseria gonorrhoeae and chlamydia trachomatis. there are more than 800,000 cases of pelvic inflammatory disease annually accounting for approximately 200,000 hospital admissions for acute and chronic infections. early accurate diagnosis and treatment are essential to prevent the serious sequelae including ectopic pregnancy, tubal disease infertility, chronic pain, and disability requiring multipl ...	1992	1391639
identification and characterization of osteoclast progenitors by clonal analysis of hematopoietic cells.	we have identified a distinct population of colony-forming cells that give rise to mononuclear cells expressing an enzyme marker and other features of the osteoclast in bone marrow cultures stimulated by conditioned medium of a murine tumor cell line. these colony-forming cells were defined as osteoclast colony-forming units (cfu-o). the tumor cell-derived activity was recently isolated and was named osteoclast colony-stimulating factor (o-csf). to understand the development of osteoclast progen ...	1992	1391940
a novel temporal expression pattern of three c/ebp family members in differentiating myelomonocytic cells.	members of the ccaat/enhancer binding protein (c/ebp) family have been shown to regulate the terminal differentiation of adipocytes and hepatocytes. in these cell lineages, high levels of c/ebp alpha are found only in mature, nondividing cells. using western blotting and immunohistochemical staining, we have determined the temporal order of expression for c/ebp alpha, c/ebp beta, and c/ebp delta in differentiating myelomonocytic marrow cells. these studies show a unique temporal pattern of c/ebp ...	1992	1391942
chlamydia trachomatis peritonitis: report of a patient presenting spontaneous regression of ascites.	a 36-year-old japanese woman complained of right hypochondralgia followed by ascites. paracentesis showed a turbid, straw-colored sterile exudate. computed tomography and magnetic resonance imaging of the abdomen revealed a left periuteric mass and ascites. the mass and ascites spontaneously regressed within a month with no specific treatment. later, after the patient had been discharged from hospital, immunofluorescence antibody titers for chlamydia trachomatis were successfully determined usin ...	1992	1392190
effects of underwater noise on human hearing.	hearing conservation for divers and swimmers has been overlooked nearly everywhere in the world. because submerging a listener changes his or her auditory physiology dramatically, the research upon which we base exposure limits for airborne noise is not pertinent under water. of the research that is necessary for developing a damage-risk standard for underwater noise exposure, only a negligible amount has been done. the value of poland's marine industries and the motivation of its audiologists m ...	1992	1392661
components and defects of the coagulation system.	the purpose of this review article is to present a simplified view of coagulation in five parts (vessels, platelets, cascade, natural inhibitors, and fibrinolysis), update the np with current concepts regarding coagulation, discuss cellular and protein functions, and provide an overall understanding of how the five components work together. discussion of disease states, genetic deficiencies, and acquired inhibitors of the coagulation system is included in this article.	2006	1392673
victory over pain: an historical perspective.	horace wells, a dentist, is credited with the discovery of anesthesia. however, there are others who experimented with inhalation agents long before wells' time. this paper reviews the history of anesthesia and recounts its discovery by wells in 1844 as we approach the 150th anniversary of the event.	2006	1392683
vitamins face control worldwide.		2006	1393127
[detection of chlamydia trachomatis in clinical material 1989-1990].	examination of 276 smears and scrapings from the uterine cervix of women before delivery and during the puerperium revealed in 28.0% positive findings. perinatal infection was confirmed by the presence of chlamydia trachomatis in 35.5% neonates with conjunctivitis and 27.3% positive smears from the nasopharynx. the authors used the direct immunofluorescence method. for immunofluorescent staining they used chlamyset of orion co., finland.	1992	1394476
detection of chlamydia trachomatis by the polymerase chain reaction in young patients with acute epididymitis.	specimens from 11 patients presenting with acute epididymitis were tested for the presence of chlamydia trachomatis by an enzyme immunoassay (eia), growth in mccoy cells and the polymerase chain reaction (pcr), and for other microorganisms by standard laboratory techniques. chlamydia trachomatis urethral infection was detected in four patients by tissue culture, in three patients by eia and in nine patients by pcr. these findings confirm the usually low detection rate of chlamydia trachomatis by ...	1992	1396770
a study of the incidence of urogenital chlamydia trachomatis in patients attending specialized departments of rome, milan and turin, italy.	a study of chlamydia trachomatis was conducted in 5270 subjects seen at clinics of the faculty of medicine and surgery at la sapienza university in rome, the s. anna hospital in turin, and the provincial maternity hospital institute in milan. in these areas, c. trachomatis was present in 5.8% of the cases examined; in addition it was present with statistically significant frequencies in cases of salpingitis (49.1) and epididymitis (21.7). it may also be found in cases of extrauterine pregnancy, ...	1992	1397232
inflammation on the cervical papanicolaou smear: the predictive value for infection in asymptomatic women.	the clinical significance of inflammation on the cervical papanicolaou (pap) smear of asymptomatic women is unknown. this study assessed the possible association between inflammation on pap smears with the presence of cervical/vaginal pathogens.	1992	1397815
[celioscopic cholecystectomy. a survey of the french society of endoscopic surgery and operative radiology. apropos of 937 cases].	twenty-eight surgeons, members of the société française de chirurgie endoscopique et de radiologie opératoire, took part in this multicenter study, carried out between march 1989 and january 1991. nine hundred and thirty-seven patients were entered into the study, 934 of whom presented with biliary lithiasis and 3 with gallbladder polyps. biliary colic was found in 918 (98 percent) of patients. one hundred and twenty-five patients (13.3 percent) presented with acute cholecystitis. laparoscopic c ...	2006	1397847
complete sequence of the major outer membrane protein-encoding gene of chlamydia trachomatis serovar da.	the complete nucleotide sequence of the gene omp1 encoding the major outer membrane protein (momp) of chlamydia trachomatis serovar da was determined following amplification by the polymerase chain reaction. the most closely related complete sequence published to date is that encoding the c. trachomatis l1 momp. when the l1 and da momp deduced amino acid (aa) sequences were compared, 16 single-aa differences located mostly in the variable domains i, ii, and iv were detected. these regions contai ...	1992	1398119
the sequence of the gene encoding elongation factor tu from chlamydia trachomatis compared with those of other organisms.	nucleotide (nt) sequences encoding the elongation factor tu (ef-tu), trna(thr) and trna(trp) from chlamydia trachomatis have been determined. the environment of the ef-tu-encoding gene (tuf), between two trna gene sequences, suggests that it is part of a tufb locus. the nt sequence and the deduced amino acid (aa) sequence were aligned with comparable sequences from other organisms and the resulting data bases were used to infer phylogenies. phylogenetic trees based on aa sequences and nt sequenc ...	1992	1398121
chlamydia trachomatis and oral contraceptive use: a quantitative review.	chlamydia trachomatis is now recognised as a major sexually transmitted disease; oral contraceptive use is rapidly increasing particularly in developing countries. there are thus important public health implications of the many reports that isolation of c trachomatis is more frequent among users of oral contraceptives. the aim of this analysis was to assess the strength and consistency of this association by summarising published studies between 1972 and 1990.	1992	1398654
effect of gamma interferon on resolution of murine chlamydial genital infection.	mice infected in the genital tract with the chlamydia trachomatis agent of mouse pneumonitis were treated with monoclonal rat anti-gamma interferon (anti-ifn-gamma) antibody to determine whether ifn-gamma participated in the resolution of the infection. in two experiments, anti-ifn-gamma antibody treatment resulted in significantly prolonged infections. in support of these data, passive administration of recombinant ifn-gamma to chronically infected nu/nu mice was able to bring about resolution ...	1992	1398955
staining of surface antigens of chlamydia trachomatis l2 in tissue culture.	surface labeling of chlamydial elementary and reticulate bodies in l929 cells infected with chlamydia trachomatis serotype l2 was monitored by using monoclonal antibodies (mab) against the major outer membrane protein and lipopolysaccharide (lps). different staining and fixation procedures were used to detect these surface antigens during the developmental cycle. anti-major outer membrane protein mab yielded a clear staining pattern of exclusively chlamydial inclusions independent of the fixatio ...	1992	1398957
identification of chlamydia trachomatis antigens by use of murine t-cell lines.	chlamydia-specific short-term t-cell lines were used in conjunction with immunoblot techniques to examine chlamydia trachomatis proteins for t-cell-stimulatory activity. this study was undertaken because of the known role of t cells in the resolution and pathogenesis of chlamydial infections. therefore, determination of which chlamydial proteins are t-cell antigens and whether they evoke protective immunity or contribute to immunopathology is crucial. immune lymph node cells were stimulated with ...	1992	1398973
disposition of roxithromycin in the epididymis after repeated oral administration.	the epididymal penetration of roxithromycin was studied in order to evaluate the drug for use in the treatment of epididymo-orchitis. seventeen patients hospitalized for surgery as part of treatment for prostatic adenoma or prostatic cancer were premedicated orally with roxithromycin 150 mg bd for three days followed by 150 mg pre-operatively (3 h before surgical incision). roxithromycin concentrations in serum and epididymis were determined by microbiological assay. the mean epididymal concentr ...	1992	1399928
secondary nerve reconstruction.	presented is a review of nerve grafting, including the indications, technique, and results. alternative techniques, including vascularized nerve grafts, tubulization, nerve elongation, and direct muscle neurotization are also discussed.	2006	1400608
effects of ascorbic acid on chlamydia trachomatis infection and on erythromycin treatment in primary cultures of human amniotic cells.	ascorbic acid (vitamin c) is an essential nutrient for humans. it may also be needed by chlamydia trachomatis, an intracellular bacterium. we investigated the effects of vitamin c on the growth of c. trachomatis e/uw-5/cx in a primary culture of human amniotic epithelial cells. the results showed that vitamin c enhances c. trachomatis infection at concentrations of 0.2, 0.6, and 1.2 mg/dl (p less than 0.001). these three concentrations represent the in vivo concentrations of deficiency, normal, ...	1992	1400952
confirmatory polymerase chain reaction testing for chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men.	first-void urine specimens from 683 men (592 without symptoms) were tested for chlamydia trachomatis by a polymerase chain reaction (pcr) with kl1 and kl2 plasmid primers and by a chlamydiazyme enzyme immunoassay (eia). thirty-seven specimens were confirmed to be positive by using the eia blocking reagent and a second set of plasmid primers (t1 and t2). by comparing unconfirmed pcr results (kl1 and kl2 primers only) with the blocked chlamydiazyme eia results, the sensitivity and specificity of p ...	1992	1400986