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cloning and characterization of the acyl-coenzyme a: 6-aminopenicillanic-acid-acyltransferase gene of penicillium chrysogenum.a gene, aat, encoding acyl-coa: 6-aminopenicillanic acid acyltransferase (aat), the last enzyme of the penicillin (pn) biosynthetic pathway, has been cloned from the genome of penicillium chrysogenum as-p-78. the gene contains three introns in the 5'-region and encodes a protein of 357 amino acids with an mr of 39,943. it complements mutants of p. chrysogenum deficient in aat activity. the aat gene is expressed as a 1.15-kb transcript and the encoded protein appears to be processed post-translat ...19892555269
comparative in vitro and in vivo activities of piperacillin combined with the beta-lactamase inhibitors tazobactam, clavulanic acid, and sulbactam.tazobactam (ytr-830h), a novel beta-lactamase inhibitor, was compared with clavulanic acid and sulbactam for enhancement of the activity of piperacillin against beta-lactamase-producing, piperacillin-resistant clinical isolates. piperacillin mics were determined in media containing a fixed concentration of 2 or 4 micrograms of the inhibitors per ml. the higher concentration was generally more effective. tazobactam was superior to sulbactam in enhancing the spectrum and potency of piperacillin. a ...19892558615
functional analyses of proteus mirabilis wild-type and mutant recbcd enzymes in escherichia coli reveal a new mutant phenotype.cloned into escherichia coli the recb, recc and recd genes of proteus mirabilis produce a recbcd enzyme (exov) functional in recombination and dna repair. the direction of transcription of recb, recc and recd, the sizes of the enzyme subunits, and their composition in the active enzyme are similar to that observed for the e. coli enzyme. in lambda crosses, the p. mirabilis enzyme has only about 40% of the chi activity of the e. coli enzyme. the recbcd genes were also cloned from an exov mutant o ...19892560121
studies to optimize the in vitro testing of piperacillin combined with tazobactam (ytr 830).the combination of piperacillin and the beta-lactamase inhibitor tazobactam (formerly ytr 830) was studied to determine optimal disk concentrations and dilution testing conditions. in addition, the potency of the combination was compared to that of piperacillin alone. the spectrum of piperacillin was greatly expanded by the addition to tazobactam principally against beta-lactamase producing strains of haemophilus influenzae, escherichia coli, morganella morganii, proteus vulgaris, providencia st ...19892560422
[proteiform syndrome (proteus syndrome). skin manifestations in a recently observed case].the case of a 4-year-old female affected by partial gigantism of the feet, syndactyly and polydactyly, partial right hemihypertrophy of buttock and lower limb, warty hyperpigmented nevus and vulvar lipoma is described. the authors discuss about the proteus syndrome, pointing out its rarity, the polymorphism and the problems of differential diagnosis with the klippel-trenaunay-weber syndrome and with other congenital hamartomatous disorders.19892560769
[the efficacy of oral ciprofloxacin in the treatment of proteus mirabilis infections of the lower respiratory tract]. 19892562708
[bacteriological, pharmacokinetic and clinical studies on cefpodoxime proxetil in the pediatric field].bacteriological, pharmacokinetic and clinical studies on cefpodoxime proxetil (cpdx-pr, cs-807), a new oral cephem antibiotic, were carried out in the field of pediatrics. the results obtained are summarized as follows. 1. antibacterial activities of r-3746 (na-salt of cefpodoxime (cpdx] against clinically isolated strains of streptococcus pneumoniae, streptococcus pyogenes, staphylococcus aureus, enterococcus faecalis, branhamella catarrhalis, escherichia coli, proteus mirabilis and haemophilus ...19892562789
ammonium assimilation in proteus vulgaris, bacillus pasteurii, and sporosarcina ureae.no active uptake of ammonium was detected in proteus vulgaris, bacillus pasteurii, and sporosarcina ureae, which indicates that these bacteria depend on the passive diffusion of ammonia across the cell membrane. in p. vulgaris the glutamine synthetase-glutamate synthase (gs-gogat) pathway and glutamate dehydrogenase (gdh) were present, and these enzymes exhibited high affinities for ammonium. in b. pasteurii and s. ureae, however, no gs activity was detected, and gogat activity was only present ...19892570557
usefulness of trehalose fermentation and l-glutamic acid decarboxylation for identification of biochemically aberrant providencia stuartii strains.a total of 849 providencia isolates were collected during a 4-year period when an increased incidence of nosocomial providencia stuartii infection was noted in urologic wards. of these isolates, 630 were identified as p. stuartii, 206 were identified as providencia rettgeri, and 1 was identified as providencia alcalifaciens. twelve inositol-positive isolates from 10 patients (10 strains) resembled p. stuartii in fermenting trehalose but resembled p. rettgeri in fermenting d-arabitol or meso-eryt ...19892570791
[morphological properties and adhesiveness of bacteria of the genus proteus].out of 100 proteus strains isolated from patients with purulent inflammatory, urological and enteric diseases, from healthy persons and from the environment, 29 stains showed the positive d-mannose-resistant reaction of hemagglutination with chick red blood cells and 18 strains showed such reaction with goose and duck red blood cells. the results of these studies permit the use of chick red blood cells as target cells for the detection of proteus adhesin. human red blood cells of groups o, a, b ...19892572096
the haemagglutinins and fimbriae of proteus penneri.the haemagglutinins and fimbriae produced by 18 strains of proteus penneri were studied and compared with those formed by representative strains of other species of proteeae. after repeated subcultures at 30 degrees c, 12 p. penneri strains formed only mr/k haemagglutinins which were associated with thin, non-channelled, type-3 fimbriae. two strains formed simultaneously both ms and mr/k haemagglutinins associated with thick, channelled, type-1 fimbriae and type-3 fimbriae, respectively. four st ...19892574748
sharing of antigenic determinants between the nicotinic acetylcholine receptor and proteins in escherichia coli, proteus vulgaris, and klebsiella pneumoniae. possible role in the pathogenesis of myasthenia gravis. 19852578213
antigen-specific anti-phosphocholine antibodies: binding site studies.the present investigation extends our initial evaluation of the evolution of antigen selection mechanisms for antibodies of a "single" specificity. the binding sites of 11 mouse anti-pc antibodies produced in response to the bacterium p. morganii or the nematode a. suum were characterized for both hapten and hapten plus carrier specificity. all of the anti-p. morganii hp belonged to the m603 anti-pc antibody family, whereas all the a. suum hp belonged to the m511 family. of the eight anti-p. mor ...19852579145
homologies between paraflagellar rod proteins from trypanosomes and euglenoids revealed by a monoclonal antibody.a nonidet p 40 insoluble fraction was isolated from trypanosoma brucei and was used to raise a monoclonal antibody (5e9). the antigen was localized by indirect immunofluorescence in the flagellum of t. brucei and of two species of euglenoids, euglena gracilis and distigma proteus. in immunoblot analysis, 5e9 appeared to bind to paraflagellar rod proteins pfr1 and pfr2 of t. brucei (72000 and 75000 mol. wt.) and of e. gracilis (67000 and 76000 mol. wt.). the presence of a common epitope in parafl ...19852581783
temperature-dependent incorporation of 4-amino-l-arabinose in lipid a of distinct gram-negative bacteria.the presence and the relative amount of 4-amino-l-arabinose in lipopolysaccharides of members of the enterobacteriaceae family and in a single strain of chromobacterium violaceum has been studied with regard to growth-temperature dependent variations. changes in the presence and the amount of 4-amino-l-arabinose (4-aa) were observed in almost all cases, but the variations observed were not consistent among different species. while salmonella minnesota and proteus mirabilis showed higher levels o ...19892583477
microbiology of infected pilonidal sinuses.aspirates of pus from infected pilonidal sinuses in 75 patients showed bacterial growth. anaerobic bacteria only were recovered in 58 (77%) specimens, aerobic bacteria only in three (4%), and mixed aerobic and anaerobic bacteria in 14 (19%). two hundred and nine isolates were recovered: 147 anaerobes (2.0 isolates a specimen) and 62 aerobes (0.8 a specimen). the predominant anaerobes were bacteroides sp (81 isolates, including 29 bacteroides fragilis group) and 51 anaerobic cocci. the predominan ...19892584424
hygienic status of food handlers.the study demonstrated bacterial species on hands and nails of food-handlers before and after hand-washing. those were staphylococcus spp., streptococcus spp., micrococcus spp., bacillus spp., diphtheroid, aeromonas hydrophila, klebsiella pneumoniae, acinetobacter, enterobacter cloacae, escherichia coli, pseudomonas spp., proteus mirabilis, serratia spp., citrobacter freundii. before hand washing, each food-handler harboured one to eight bacterial species. after hand-washing (eight with water fr ...19892584903
comparison of two endometrial sampling devices. cotton-tipped swab and double-lumen catheter with a brush.the potential for bacterial contamination during transvaginal recovery of endometrial samples in patients with postpartum endomyometritis spawned an investigation into various protective measures and techniques. the confirmation and determination of the extent of contamination was attempted in 120 postpartum women with the diagnosis of postpartum endometritis. each woman underwent endometrial sampling for bacterial culturing utilizing two devices--an unprotected, cotton-tipped swab and a protect ...19892585387
[immunotherapy with solco urovac vaccine in chronic genitourinary infection].twenty five patients with chronic urogenital infections (cystitis, pyelitis and prostatitis) were treated with solco urovak infection which is active against escherichia coli, proteus, klebsiella and streptococcus fecalis organisms. this microbial flora has been isolated from these patients and proved resistant to the conventional antibacterial agents. the vaccine was very well tolerated, gave no side reactions, but transient short lasting rise of the temperature to 38 degrees c. the bacteriolog ...19892585977
[purulent meningitis of rhinogenic or otogenic origin--a contribution to the clinical problem]. 19892588676
ciprofloxacin in patients with bacteremic infections. the spanish group for the study of ciprofloxacin.the efficacy and safety of ciprofloxacin in the treatment of 68 episodes of bacteremia were studied. patients were treated intravenously (30 cases), orally (13 cases), or with sequential intravenous/oral therapy (25 cases). intravenous doses ranged from 200 to 400 mg per day and oral doses ranged from 1,000 to 1,500 mg per day. according to the criteria of mccabe and jackson, 39 cases had nonfatal and 29 had ultimately fatal underlying diseases. the clinical condition of patients at the start of ...19892589366
comparative in vitro activity and beta-lactamase stability of fk482, a new oral cephalosporin.fk482 is an oral aminothiazolyl hydroxyimino cephalosporin with a c-3 vinyl group. its activity was compared with those of cephalexin, cefuroxime, cefixime, and amoxicillin-clavulanate. fk482 inhibited 90% of staphylococcus aureus isolates at 1 micrograms/ml and 90% of streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae isolates at less than or equal to 0.012 micrograms/ml, superior to cephalexin and cefuroxime and similar to cefixime. it did not inhibit oxacillin-resi ...19892589845
[effect of sisomicin on intestinal microflora and colonization resistance in rats and their offspring].in experiments with wistar female rats it was shown that after administration in the form of inhalations (10.87 mg/m3) sisomicin was present in the animal feces in a concentration of 26.6 micrograms/g. prolonged exposure to the antibiotic during the pregnancy term led to changes in microbiocenosis of the animal large intestine which was observed even a month after the delivery. the progeny of the animals was characterized by the presence of significant numbers of aerobic bacteria resistant to si ...19892589899
evaluation of stuart's transport medium containing penicillinase. an in vitro study. brief report.an in vitro study was performed to evaluate the value of penicillinase in stuart's transport medium. swabs were taken from serum broth cultures of staphylococcus aureus, escherichia coli and proteus rettgeri, incubated with cefuroxime. following 24 hours' storage in the transport medium, the recovery of p. rettgeri was significantly higher from stuart's transport media containing penicillinase in a concentration of 100,000 units/ml, than from swabs stored in stuart's medium without penicillinase ...19892590536
increased urinary nitrosamine excretion in patients with urinary diversions.tumor development at the site of ureterointestinal anastomosis is a recognized complication in patients with continent urinary diversions. aerobic cultures of rectal urine samples from 30 patients with urinary diversions (26 ureterosigmoidostomies, two colon conduits, one ileal conduit and a gersuny bladder) showed a complex bacterial flora containing nitrate-reducing organisms (escherichia coli, proteus and klebsiella spp.). in comparison to normal bladder urine samples from control volunteers ...19892591028
mechanism of beta-lactamase inhibition: differences between sulbactam and other inhibitors.three different types of beta-lactamases--tem-2 type penicillinase, a typical cephalosporinase of citrobacter freundii, and a proteus vulgaris cephalosporinase with broad substrate range--were studied to determine the inactivation and reactivation kinetics for beta-lactamase inhibitors of these enzymes. sulbactam, cloxacillin sulfone, clavulanic acid, imipenem, and aztreonam were evaluated. on the basis of the kinetic parameters a minimum scheme for the inactivation of these beta-lactamases by e ...19892591172
sulbactam: secondary mechanisms of action.light and scanning electron microscopy showed that 0.25, 0.5 and 1 times the minimum inhibitory concentrations (mics) of sulbactam (sulb) caused filament formation in different species of enterobacteriaceae, while 2 and 4 times the mics caused spheroplast formation and subsequent lysis. by using a competitive assay with 125i-penicillin x, sulb showed a primary affinity for the pbp 1a and pbp3 of escherichia coli, as well as for the pbp1a of proteus mirabilis. the bactericidal interaction of huma ...19892591173
antibiotic resistance in australia with special reference to sulbactam/ampicillin.a survey of antibiotic resistance in australian states was undertaken by the microbiology quality assurance program of the royal college of pathologists of australasia. data were obtained from hospitals and private pathology laboratories serving both in-patients and out-patients at community hospitals. the study showed that resistance varied from state to state; it was highest in the eastern states of new south wales, victoria, and queensland, and lowest in tasmania and western australia. in sou ...19892591174
[assessment of the prophylactic activity of staphylococcal anatoxin and proteus vaccine in the combined treatment of patients with trauma to the locomotor system]. 19892594344
[calcification and contamination as complications in the use of double-j catheters: incidence and correlation].we present a series of cases of complex renal lithiasis, in which we carried out temporary internal urinary bypassing, prior to extracorporeal lithotripsy, by means of the retrograde insertion of a double-j urethral catheter, studying the time of stay, the presence or not of contamination in the catheter and its relationship with the calcifications found in them. we also carried out a metabolic-functional study of each patient, determining the urinary ph and the serum and urinary calcium, phosph ...19892596357
infection of hemodialysis catheters: incidence and mechanisms.fifty-three consecutive subclavian or jugular hemodialysis catheters inserted into 41 patients were prospectively studied over a period of 8 months in order to determine the incidence of infection and its mechanisms. the intravascular, intradermal and the y catheter segments as well as both connections were cultured using a quantitative technique for the intraluminal surface. in addition, the intravascular and intradermal portions of the catheter were cultured using a semiquantitative technique ...19892596535
comparative in vitro encrustation studies of biomaterials in human urine.a new dynamic in vitro human urine model was developed to compare biomaterial encrustation. the model incorporates a capacity to study seven biomaterials, a daily urine inflow of 500 ml, a reservoir capacity of 700 ml, and a turnover rate of four days. encrustation studies performed for 2 weeks in sterile and infected (proteus vulgaris) urine on segmented polyether polyurethane, polyester polyurethane, silicone (mitsui), silicone (dow corning), biothane, biolor 1 and biolor 11 demonstrated that ...19892597516
antibacterial activity of norfloxacin against 1700 relatively resistant clinical isolates.a total of 1724 clinical isolates from patients with different infections were tested in vitro to determine their susceptibility to norfloxacin. antibacterial activity of norfloxacin was compared with other drugs that were commonly used in the hospital. of the 919 strains of enterobacteriaceae tested, all except four isolates of enterobacter, two of serratia and one of proteus were susceptible to norfloxacin. ninety-five percent of the 199 strains of pseudomonas aeruginosa were also inhibited by ...19892598776
the effects of vasopressin and related peptides on osmoregulation in amoeba proteus.we describe the effects of arginine-vasopressin (avp) and five related peptides on the contractile vacuole, the osmoregulatory organelle of the fresh water amoeba proteus. arginine-vasopressin, lysine-vasopressin, and skf 101926, a synthetic antagonist of vasopressin, cause a significant increase in the rate of output of the contractile vacuole. deamino-vasopressin (davp), oxytocin, and arginine-vasotocin have no such activity, although davp interferes with the action of avp when present in equi ...19892599342
a contribution to the situation of antibiotic resistance in abeokuta (ogunstate) and minna (nigerstate) in nigeria.after testing various chemotherapeutics the following conclusions could be drawn: pseudomonas was sensitive only to gentamicin. gentamicin, aminopenicilline + calvulanacid and cefoxitin were 100% effective against e. coli. gentamicin also proved effective against enterobacter (83%). cefoxitin, aminopenicillin + clavulanacid, gentamicin and trimethoprim + sulfonamide were effective against klebsiella. concerning proteus sp., cefoxitin showed best results (100%). acinetobacter was 100% inhibited b ...19892599386
antigenic reactivity of a monoclonal antibody against amoeba proteus actin.the reactivity of a monoclonal antibody against actin of amoeba proteus with actins from other sources was examined. the monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and acanthamoeba castellanii, but it did not react with naegleria gruberi actin. the amoeba actin was resolved into 3 bands with isoelectric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2-dimensional immunobl ...19892600879
clavulanate and beta-lactamase induction.concern has been expressed that clavulanate can antagonize ticarcillin against enterobacteria and pseudomonads that have inducible expression of chromosomal 'class i' beta-lactamases. it is suggested that clavulanate-induced enzyme inactivates ticarcillin, which itself is a feeble inducer. we confirmed that this mechanism applied, showing that antagonism was abolished in beta-lactamase-basal mutants of inducible strains. antagonism has been reported in double disc tests with strains of pseudomon ...19892606817
the line blot: an immunoassay for monoclonal and other antibodies. its application to the serotyping of gram-negative bacteria.a procedure is described for assaying antibodies based on the application of antigen to nitrocellulose as a line with an ink pen point. the method requires no expensive apparatus, is easy to perform, and requires less than 0.25 micrograms of antigen per assay. more than 45 antigens can be assayed simultaneously with a single antibody. antigens can be applied as purified proteins, extracts, or sodium dodecyl sulfate solubilized extracts. the application of the line blot assay for the detection of ...19892607166
[comparative studies on activities of antimicrobial agents against causative organisms isolated from urinary tract infections (1986). iii. secular changes in susceptibility].changes in the susceptibility of various infectious microorganisms to antimicrobial agents from 1982 to 1986 were evaluated. the microorganisms investigated were escherichia coli, klebsiella spp., citrobacter spp., enterobacter spp., proteus spp., serratia marcescens and pseudomonas aeruginosa isolated from patients with urinary tract infections. we compared susceptibilities of microorganisms obtained from simple urinary tract infections with those from complicated infections with or without ind ...19892607604
[microbiological findings of 97 cases of acute pyelonephritis].to optimize the therapeutic approach to acute pyelonephritis (pna), the medical records of 89 patients (73 women and 16 men) hospitalized with diagnosis of pna between january 1984 and 1987 have been studied. according to routine bacteriological urinalysis, gram-negatives were found in more than 92% (e. coli in 79%, proteus in 6%). gram-positives were found in only 8%. the gram-negatives (n = 82) showed resistance to amoxicillin in 36%, to amoxicillin/clavulanic acid in 17%, to cotrimoxazole in ...19892609141
[microflora of gastric juice and its sensitivity to antibiotics in post-gastrectomy gastritis].fifty four patients with postresection gastritis and 13 healthy adults were examined. 141 microbial cultures belonging to 33 species were isolated from the gastric juice of the patients. in the healthy persons 27 cultures belonging to 7 species were isolated from the gastric juice. in the gastric juice of the patients there predominated enteric bacteria and enterococci (64.8 +/- 6.5 and 57.4 +/- 6.7 per cent respectively). among the enteric bacteria escherichia coli and proteus spp. were the mos ...19892610535
bacteriology and drug sensitivity of chronic suppurative otitis media at a central hospital in zimbabwe.a retrospective study was done on 173 cases of chronic suppurative otitis media at harare central hospital. using conventional pus swabs from the discharging ears only aerobic organisms were isolated in 155 cases. these were, in order of frequency, staphylococcus aureus, pseudomonas aeruginosa, lactose fermenting, coliforms and proteus. there were no anaerobes reported, unlike in other studies. mixed infections were present in 48 percent of the cases. penicillin g, ampicillin, tetracycline sulph ...19892611874
cockroaches (periplaneta americana) as carriers of agents of bacterial diarrhoea in accra, ghana.bodies and intestinal contents of 208 cockroaches (periplaneta americana), collected from kitchens in accra and some surrounding villages were cultured for enteric bacterial pathogens. six of them harboured three different serogroups of salmonella, one had shigella dysenteriae, 64 had coliforms, 13 had proteus species, two had pseudomonas species and the rest (122) carried none of the bacterial species mentioned above. the presence of salmonella species shigella dysenteriae and coliforms in thes ...19892611875
production and characterization of a compound inhibitory to vibrio parahaemolyticus from proteus vulgaris.the production and characteristics of a compound in proteus vulgaris g cultures which was capable of inhibiting vibrio parahaemolyticus and other food-borne pathogens was investigated. production was influenced by medium composition, ph and temperature but not by the extent of aeration. the compound was most inhibitory at the optimum temperature for growth of v. parahaemolyticus. the inhibitor was most stable at ph 7.0 and inhibition occurred even after heating at 70 degrees c for 30 min and aft ...19892613590
in-vitro activity of cefetamet (ro 15-8074) compared with other oral agents.the bacteriostatic and bactericidal activities of cefetamet (ro 15-8074) were compared with those of cefadroxil, cefaclor, ampicillin, doxycycline, chloramphenicol and erythromycin against a total of 400 bacterial strains isolated from nosocomial respiratory tract and auditory canal infections. the broth macrodilution method was used to determine antimicrobial susceptibility. none of the compounds was effective against pseudomonas aeruginosa and methicillin-resistant staphylococcus aureus strain ...19892613603
glutathione transferase in bacteria: subunit composition and antigenic characterization.the presence of glutathione transferase (gst; ec 2.5.1.18) in escherichia coli atcc 25922, e. coli atcc 25422, proteus vulgaris atcc 8427, pseudomonas aeruginosa atcc 27853, klebsiella oxytoca cip 666, k. oxytoca af 101, enterobacter cloacae cip 6085, serratia marcescens cip 6755, and proteus mirabilis af 2924 was investigated. using 1-chloro-2,4-dinitrobenzene as substrate, gst activity was found in the glutathione-(gsh-)affinity-purified fraction of all strains tested. sds-page analysis of gsh ...19892614380
[bacteremia secondary to decubitus ulcer].we have prospectively evaluated 16 episodes of bacteremia induced by decubitus ulcers. the most commonly isolated microorganisms were staphylococcus aureus, proteus mirabilis, pseudomonas aeruginosa and bacteroides fragilis. bacteremia was polymicrobial in 5 cases (31%). most ulcers were already present at the time of admission, but bacteremia developed within the hospital in 13 patients. in 3 patients, ulcers did not show local features of infection. the initial antibiotic therapy was satisfact ...19892615535
proteus syndrome. 19892616392
[in vitro susceptibility to a new antimicrobial agent (imipenem) of pathogens isolated from inpatients at various centers].imipenem is a beta lactam antibiotic, a highly potent new carbapenem with broad antibacterial spectrum. to test the "in vitro" efficacy of this antimicrobial agent in pathogens more frequent in several medical centers in brazil, susceptibility testing with 10 mcg imipenem disks and, or corresponding mic were carried out with 1231 recent isolates of 41 different bacterial species, obtained mainly from hospitalized patients in 5 different medical centers of the cities of s. paulo, rio de janeiro a ...19892617012
[synthesis of 7 beta-(6-substituted-2-quinolone-3-acetamido)cephalosporins].a series of new 7 beta-(6-substituted-2-quinolone-3-acetamido)cephalosporins has been prepared by acylation of the 7 beta-amino group of 7-adca, 7-aca, 7-act and 7-acd with 6-substituted-2-quinolone-3-acetic acids. cdi (n,n'-carbonyldiimidazole) method was mainly adopted and active ester method was also employed in the reactions. isolation and purification were fulfilled with the combined methods of sephadex lh-20 column chromatography and centrifugal tlc technique. sixteen new cephalosporin der ...19892618714
gas liquid chromatography of amines produced by the enterobacteriaceae.1. gas liquid chromatography (glc) was used to test the production of amines by 85 strains of enterobacteriaceae. 2. the strains tested produced cadaverine, beta-phenylethylamine, putrescine, iso-amylamine, 2-methylbutylamine and iso-butylamine. 3. although the overlap in amine production between obviously different genera and species limits the general applicability of this methodology in clinical microbiology, the results obtained demonstrate the possibility of differentiating proteus rettgeri ...19892620174
in vitro and in vivo antibacterial activity of kt3777, a new orally active carbacephem.kt3777 is a novel carbacephem antibiotic structurally identical to cefaclor (ccl), except that the sulfur atom of position 1 of the cephem nucleus has been replaced by carbon. kt3777 was investigated for in vitro and in vivo antibacterial activities in comparison with ccl, cephalexin (cex) and amoxicillin. the mic50 of kt3777 ranged from 0.2 to 3.13 micrograms/ml for clinical isolates of staphylococcus aureus, streptococci, escherichia coli, klebsiella pneumoniae, proteus mirabilis, haemophilus ...19892621166
[determination of the mic of cefotetan against freshly isolated gram-negative bacilli].antimicrobial activities (mics) of cefotetan (ctt) against 575 strains of 16 spp. of gram-negative bacilli isolated in 1988 were determined to investigate distribution of mics in comparison with those of cefmetazole (cmz), cefoxitin (cfx), latamoxef (lmox) and cefazolin (cez). the change in frequencies of incidence of cephem-resistant strains in the latter half of the 1980 was also investigated. distribution of mic of ctt varied with test strains. no or very few mics were at or higher than 12.5 ...19892621779
[clinical laboratory approach to estimating the effective administration dose of imipenem/cilastatin. evaluation of the disk susceptibility test and its interpretation system].in vitro activities of imipenem/cilastatin (ipm/cs) against 413 clinical isolates were studied through the evaluation of mics and the results of disk susceptibility tests. the mics were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. the mic80s of imipenem (ipm) against staphylococcus aureus and staphylococcus epidermidis were 25 and 1.56 micrograms/ml, respectively, showing a bimodal mic distribution. however, the distribution of mics against other bacteria studi ...19892621780
[bacteriologic monitoring in a surgical department with emergency services. epidemiologic analysis and study of sensitivity to antibiotic therapy].the two year experience with 2091 biological cultures and the related antibiograms has been reported on the basis of a prospective, computerized study. the incidence of germs coming from each fluid collected has been analyzed as well as the sensibility to various antibacterial drugs. the gram negative such as escherichia coli, enterococcus, pseudomonas, proteus, klebsiella, serratia and enterobacter resulted more frequently isolated; in addition a revival of gram positive strains (staphylococcus ...19892622558
experimental investigations on dissolution of incrustations on the surface of catheters.on the basis of a standardized in vitro irrigation model, the incrustation behavior of catheters was investigated using collected urine with controlled contamination (proteus, mirabilis). irrigation treatment with a nacl solution did not effectively reduce incrustation compared to the control experiment. on the other hand, incrustations were definitively prevented or existing crystal deposits were largely dissolved with a citrate solution of ph 4.0 (suby g). analytical investigations (infrared s ...19892623786
[a case of a large pyelic calculus composed only of ammonium urate].the authors report a case of pure ammonium urate stone. it was a very large lightly radiopaque pelvic stone, which was extracted by pyelotomy and analysed by infra-red spectrography. the clinical history did not reveal a long history of chronic infections. the stone was diagnosed by urography performed after an acute urinary trad infection. proteus mirabilis was found at one time, but only after the surgical operation. the blood and urinary laboratory check-up was normal. no case of such a large ...19892624452
[patterns of resistance of staph aureus and gram-negative bacteria to aminoglycosides and cephalosporins].the sensitivity to 4 aminoglycoside antibiotics (gentamicin, tobramycin, netilmycin and amicacin) and 5 cephalosporins (cefradine, cefamandol, cefotaxime, cefoperazone and ceftriaxone) was determined in 700 bacterial strains isolated from clinical materials in the years 1986-1987. the most frequent coexistent resistance was observed to gentamicin and tobramycin in s. aureus (30%), klebsiella (30%), proteus mirabilis (28%) and enterobacter (23%). resistance to 5 cephalosporins was found in entero ...19892629327
antimicrobial activity of dactimicin in vitro compared with that of dibekacin, netilmicin, sisomicin and micronomicin.antimicrobial activity of dactimicin, a pseudo-disaccharide aminoglycoside antibiotic, was compared with those of dibekacin, netilmicin, sisomicin and micronomicin using clinical isolates of four gram-positive and sixteen gram-negative bacteria. dactimicin was more active than the reference amino-glycosides against serratia marcescens, especially gentamicin-resistant serratia sp., proteus vulgaris, p. rettgeri and klebsiella oxytoca, but less active against pseudomonas aeruginosa and p. mirabili ...19892632214
[scrub typhus--one case report].scrub typhus (tsutsugamushi disease) is an acute infectious disease caused by rickettsia tsutsugamushi transmitted through the bite of larvae of certain trombiculid mites. geographical distribution in asian-pacific region is much of the roughly triangular area bounded by japan, pakistan and australia. it is an endemic illness in the pescadores islands, but has scarcely been reported in central taiwan. an eleven-year-old boy was admitted to changhua christian hospital with the chief complaints of ...19892634464
gall bladder disease: an analytical report of 250 cases.two hundred and fifty cases of biliary tract disease were studied as regards case history, physical and laboratory investigations, surgery and follow-up. it was found that females especially multipara were frequently affected; majority of cases were in 3rd to 5th decade of their life, rise in age showing decline in incidence. majority of cases (82.4%) were vegetarians and had used vegetable fats (oriental diet). most of the cases (98.8%) belonged to middle and poor class and were lean and thin. ...19892634700
[antibacterial action of some saturated and unsaturated long-chain carboxylic acids and their bis(2-chloroethyl)aminoethyl esters in vitro].using the method of twofold serial dilution of substances in liquid nutrient medium with subsequent reseeding on solid nutrient medium a comparative study has been carried out of the antibacterial action in vitro of long chain saturated (c10-c18) and unsaturated (c11-c22) carboxylic acids and their bis(2-chloroethyl)aminoethyl esters against pseudomonas aeruginosa, klebsiella pneumoniae tp 16 koph, escherichia coli atcc 25922, staphylococcus aureus 101+ and proteus mirabilis 56. the compounds st ...19892635322
[comparison of in vitro activity of ciprofloxacin and pefloxacin against strains isolated from clinical material].the susceptibility of 392 strains of pathogenic bacteria isolated from clinical material of hospitalized patients in institute for chest diseases golnik were studied. 100 strains of escherichia coli, 56 strains of enterobacter spp., 40 strains of proteus mirabilis, 50 strains of gram negative nonfermentative bacteria, 46 strains of pseudomonas aeruginosa and 100 strains of staphylococcus aureus were tested. minimal inhibitory concentrations of ciprofloxacin and pefloxacin were determined by agar ...19892636406
urinary tract infections in children.from january 1981 to december 1987, 346 children with urinary tract infections, proved by urine culture, were admitted to the department of pediatrics, mackay memorial hospital. the ratio of male to female was 3.0 in children below 2 years, and 0.8 in children above 2 years, of age. the urine specimens were collected from suprapubic punctures in 281 cases (81.2%). fever was the most common clinical manifestation. in children below two years old, other common symptoms and signs were body weight l ...19892637602
rapid identification of salmonella from poultry meat products by using 'mucap test'.a study was made in order to improve a new salmonella identification test (mucap test) in which umbelliferone is released, giving a blue fluorescent light under a wood lamp, after contact with salmonella colonies. the study concerned 354 colonies, previously isolated from 55 poultry meat samples. two enrichment media [tetrathionate bile broth (tbb) and rappaport vassiliadis (rv)] and two isolation media [brilliant green agar (bga) and desoxycholate agar (da)] were used, and the results of the te ...19892641489
evaluation of the cobas-bact system for direct and rapid identification and antimicrobial susceptibility testing of gram-negative rods from positive blood culture broths.a direct antimicrobial susceptibility test and a direct identification of positive blood culture broths for gram-negative rods confirmed with gram stain by using a new instrument, cobas-bact, were compared with the conventional kirby-bauer agar diffusion disk method and with the in-house set of identification or api 20e, respectively. the bacterial pellet of centrifuged positive blood culture broth was used to inoculate a cobas-bact susceptibility and identification rotor. bacteria from 206 case ...19892643613
invasion of vero cells by salmonella species.the invasiveness of salmonella strains for vero cells was studied by quantitative bacteriology; the technique was more sensitive than phase contrast microscopy. all of 59 salmonella strains, of 19 different serotypes, were more invasive than escherichia coli k12. three strains of shigella were as invasive as most of the salmonella strains whereas 29 strains of e. coli, two of proteus, three of klebsiella and one of serratia were much less invasive. two citrobacter strains exhibited intermediate ...19892643706
chronic urinary tract infection and renal stones.in patients with bacteriuria and small and otherwise asymptomatic renal stones, the infection should be linked to the stone before surgical removal of the stone is considered. in this study the level of infection was determined by ureteric catheterisation. the method could discriminate between stone-related infections (70%) and infections confined to the lower urinary tract (30%) in virtually all of the patients. even very small stones (3 mm) were shown to be the cause of decade-long bacteriuria ...19892646704
[the rapid identification of escherichia coli using bactident e. coli].165 (93.7%) of 175 e. coli strains isolated from various materials of different animal species were correctly identified as e. coli with help of commercially available bactident e. coli (e. merck, d-6100 darmstadt). further 52 strains of the family of enterobacteriaceae (2x providencia sp., 2x salmonella sp., 7 citrobacter sp., 9 proteus sp., 15 klebsiella sp., 17 enterobacter sp.) were correctly not identified as e. coli by this test. bactident e. coli is a suitable test for rapid identificatio ...19892647078
[correlation of the cause and composition of renal calculi. value of morphologic and infrared analysis].the morphological and infrared spectrophotometric analysis of the urinary stones of 300 patients have been reported in this article. calculi are classified into six morphological types with their corresponding mineralogical natures. the type i (whewellite or c1) is pure in 18 p. cent of lithiasis, more often present in the center than on the surface, with hyperoxaluria in 81 p. cent. calculi linked to piridoxilate intake (3 p. cent) have this composition. the type ii (weddellite or c2) rarely pu ...19892648901
[penile inflammation in ganders].since 1987 penis-inflammation and -prolapse were observed in north-german breeder-geese. up to 28% of the ganders showed local symptoms. in the females no clinical signs of cloaca-inflammation were seen. the general condition of the birds was good and there was no increased mortality. egg production and fertility were not influenced. bacteriological examinations of the altered penis-tissues revealed in different frequency microorganisms belonging to the genera mycoplasma, candida, bacteroides, c ...19892651081
structural studies on the fucosamine-containing o-specific polysaccharide of proteus vulgaris o19.the polysaccharide chain of proteus vulgaris o19 lipopolysaccharide contains d-galactose, n-acetyl-d-glucosamine n-acetyl-d-galactosamine and n-acetyl-l-fucosamine in the ratio 1:1:1:1. the structure of the polysaccharide was established by full acid hydrolysis and methylation analysis, as well as by non-destructive methods, i.e. the computer-assisted evaluation of the 13c-nmr spectrum and computer-assisted evaluation of the specific optical rotation by klyne's rule. the polysaccharide is regula ...19892651127
protracted diarrhea: the importance of the enteropathogenic e. coli (epec) strains and salmonella in its genesis.the etiology of the protracted diarrhea is ill-defined, but in the underdeveloped countries acute gastroenteritis might be the most common triggering factor, especially due to certain enteropathogenic bacteria, such as enteropathogenic e. coli (epec) and salmonella. we investigated the role of these agents in the genesis of protracted diarrhea in 29 infants with a mean age of 4.6 months. the patients underwent the following tests: stool culture, culture of the jejunal secretion, and small bowel ...19892651634
[bladder calculus formed on chromic suture].this is a description of a clinical case of a two-and-a-half year old male child who developed a urinary bladder stone seeded on chromic catgut used to suture of the bladder wall which was damaged in the course of the surgical repair of an inguinal hernia.19892652996
[early calculogenesis caused by ureolytic infection of the double-j ureteral catheter].stone formation in the double j ureteral catheter is an uncommon complication that generally appears in patients that are predisposed to stone formation and persistent infection of the urinary tract. we report on a 29-year-old female patient in whom stone formation was observed in both ends of the catheter, less than 3 months after it had been placed. urine culture was positive for p. mirabilis. percutaneous nephrolithotomy was warranted in the treatment of the pyelic stone and for removal of th ...19892653240
tissue penetration and clinical efficacy of enoxacin in urinary tract infections.the fluoroquinolones in general, and particularly enoxacin, show great promise in the treatment of urinary tract infection. orally administered enoxacin achieves high concentrations in the serum and urine as well as in prostate tissue, kidney and perirenal fat and muscle. these concentrations are generally in excess of the minimum inhibitory concentrations (mic) for 95% of the common uropathogens, including escherichia coli, pseudomonas aeruginosa, klebsiella spp., proteus spp., enterobacter spp ...19892653693
[neonatal proteus mirabilis septicemia and cerebral abscess. value of the assay of antibiotics in the puncture fluid].the authors describe a case of neonatal proteus mirabilis septicemia accompanied by cerebral abscess formation despite the presence of therapeutically effective antibiotic levels utilised to treat the disorder. the occurrence of such brain abscesses during the course of effective antibiotic therapy raises the question of the mechanism behind their formation. cerebritis may occur very early in the clinical course of the infection without being due to failure of antimicrobial therapy.19892654878
isolation and analysis of the c-terminal signal directing export of escherichia coli hemolysin protein across both bacterial membranes.we have studied the c-terminal signal which directs the complete export of the 1024-amino-acid hemolysin protein (hlya) of escherichia coli across both bacterial membranes into the surrounding medium. isolation and sequencing of homologous hlya genes from the related bacteria proteus vulgaris and morganella morganii revealed high primary sequence divergence in the three hlya c-termini and highlighted within the extreme terminal 53 amino acids the conservation of three contiguous sequences, a pot ...19892656259
detection of antigens from gram-negative bacilli in urine of children with urinary tract infections. ii. thermostable bacterial proteins.an enzyme immunoassay for detection of bacterial antigens in urine specimens from urinary tract infections (uti) was developed. the antigens detected in infections by enterobacteriaceae: escherichia coli, enterobacter aerogenes, and proteus sp. were different from the enterobacterial common antigen (eca). they were digested by trypsin and were characterized by remarkable thermostability; their molecular weights were approximately 34,000 and 31,000. they were detected by means of antisera to eca ...19892656535
review of the in-vitro spectrum and characteristics of cefmetazole (cs-1170).the in-vitro antimicrobial qualities of cefmetazole are summarized from a review of over 30 publications. cefmetazole, a 7 alpha-methoxy cephalosporin, is shown to have an antimicrobial spectrum closely resembling cefoxitin's. however, cefmetazole is approximately two- to eight-fold more active than cefoxitin against commonly isolated species such as escherichia coli, klebsiella spp., proteus mirabilis, staphylococcus aureus, pyogenic streptococci, pneumococci, and haemophilus influenzae. cefmet ...19892656622
effects of beta-lactamases and omp mutation on susceptibility to beta-lactam antibiotics in escherichia coli.four types of beta-lactamases consisting of a penicillinase type i (tem-1), a penicillinase type ii (oxa-1), a cephalosporinase of citrobacter freundii, and a cephalosporinase of proteus vulgaris were introduced into escherichia coli mc4100 and its omp mutants, mh1160 (mc4100 ompr1) and mh760 (mc4100 ompr2), by transformation. effects of the combination of the omp mutations and these beta-lactamases on the susceptibility of e. coli strains were studied with 15 beta-lactam antibiotics including c ...19892658786
effects of microamperage, medium, and bacterial concentration on iontophoretic killing of bacteria in fluid.prevention of nosocomial urinary tract infections by iontophoresis is addressed. an iontophoretic generator was used to provide microamperage (10 to 400 microa) to vials containing either synthetic urine or supplemented synthetic urine. bacteria were added to vials, and parameters of growth, bacterial killing, and multiple electrode materials were examined. escherichia coli and proteus species were both inhibited and killed at various microamperages and with several electrode types, the most eff ...19892658791
aztreonam in the treatment of urinary tract infections: a multicenter trial.a multicenter trial involving several urologic units in italy provided pooled data on 1,427 patients with urinary tract infections to evaluate the efficacy and safety of aztreonam, a new monobactam antibiotic. microbiologic and clinical data were collected methodically in all cases. a majority of the patients (79.9%) were hospitalized during the study period, an associated pathology was noted in 29.1%, and 16.7% were receiving additional therapy. aztreonam was administered by different routes an ...19892659294
monoclonal antibody-mediated protection and neutralization of motility in experimental proteus mirabilis infection.a panel of monoclonal antibodies with specificity for a wound isolate of proteus mirabilis was established. of nine antibodies studied in detail, three were broadly reactive with various proteus isolates, while six reacted in a serotype-specific fashion with the strain used for immunization. five of the six serotype-specific antibodies were reactive with lipopolysaccharide. the sixth serotype-specific antibody, 4-f (immunoglobulin g1 [igg1]), was potently protective in a burn wound sepsis model ...19892659528
paradoxical resistance to ceftazidime and aztreonam in proteus mirabilis. 19892659567
monoclonal antibodies against three different enterobacterial outer membrane proteins. characterization, cross-reactivity, and binding to bacteria.balb/c mice were immunized with whole-cells of escherichia coli 055:b5 or proteus mirabilis nctc 60 to produce broadly cross-reacting monoclonal antibodies (mabs) against outer membrane (om) proteins. a total of 10 anti-om mabs of the igg class were selected. these included 5 mabs against the heat-modifiable (hm) protein, 3 against the peptidoglycan-associated lipoprotein (palp), and 2 against braun's lipoprotein (blp). based on competition elisa, the mabs defined 2 hm protein binding sites (hm ...19892660858
n-terminal region of proteus mirabilis glutathione transferase is not homologous to mammalian and plant glutathione transferases.the n-terminal amino acid sequence of glutathione transferase, pm-gst-6.0, purified from proteus mirabilis [(1988) biochem. j. 255, 971-975] up to residue 38 and a comparative peptide fingerprint are reported. no obvious homology with the sequences of alpha, pi and mu classes of mammalian glutathione transferases as well as with those of plant glutathione transferases has been noted. these results suggest that the classification so far adopted for glutathione transferases cannot be extended to t ...19892661269
heterologous signal peptide processing in fusion interferon synthesis by engineered l-forms of proteus mirabilis.a recombinant dna proteus mirabilis l-form expression system, lvi (pjs127), was used to synthesize human fusion interferon alpha 1 (f-ifn-alpha 1). in the expression plasmid used, the complete coding sequence of ifn-alpha 1 was linked to the streptococcal spea promoter and the 5' end of the spea structural gene including its signal sequence coding region. lvi (pjs127) was capable of complete secretion into the culture medium of biologically active f-ifn-alpha 1 whose identity was confirmed by im ...19892661322
adjuvant fosfomycin medication in chronic osteomyelitis.the therapeutic effectiveness of adjuvant therapy with fosfomycin was studied in a prospective clinical trial of 60 patients suffering from chronic post-traumatic osteomyelitis. the patients were aged between 17 and 78 years (mean 37.4 years). the chronic osteomyelitis was predominantly located in the tibia (43 patients) and in the femur (13 patients). most of the pathogens isolated were staphylococcus aureus (42%), coagulase-negative staphylococci (19%), pseudomonas aeruginosa (12%), streptococ ...19892661439
comparison of the in vitro and in vivo antibacterial activities of cefepime (bmy-28142) with ceftazidime, cefuzonam, cefotaxime and cefmenoxime.cefepime (bmy-28142), a new semisynthetic cephalosporin, was evaluated for in vitro and in vivo antibacterial activities in comparison with ceftazidime, cefuzonam, cefotaxime and cefmenoxime. cefepime showed a well-balanced, broad spectrum of activity against a number of clinical isolates collected in japan. the activity of cefepime against gram-positive bacteria was several times greater than that of ceftazidime, nearly comparable to cefotaxime and cefmenoxime, and slightly weaker than cefuzona ...19892663406
recurrent meningitis: a case report.a 25-month-old girl is described who experienced three successive attacks of purulent meningitis due to proteus mirabilis. third generation cephalosporins were employed as treatment. cranial radiography and computed tomography revealed a malformation, in the form of an epidermoid cyst, in a central occipital position; small cerebellar abscesses were also present. the lesion was removed by surgery which led to a complete recovery.19892663517
interaction of the bisbiguanides chlorhexidine and alexidine with phospholipid vesicles: evidence for separate modes of action.strains of providencia stuartii with demonstrated resistance towards chlorhexidine did not show such resistance towards either of the related biguanide antiseptics, alexidine or vantocil. alexidine promoted a significantly faster alteration in the permeability of escherichia coli cell membranes towards various metal cations than chlorhexidine. differential thermal analysis of various mixed lipid vesicles and pure phospholipid vesicles showed alexidine to share with vantocil the property of produ ...19892663802
activities and sources of beta-lactamase in sputum from patients with bronchiectasis.beta-lactamase activity was measured in secretions from patients with bronchiectasis. of 28 sputum samples, 23 contained measurable amounts of activity; values were significantly higher (p less than 0.01) in purulent samples than in mucoid or mucopurulent samples. beta-lactamase activity was usually present in saliva collected before and between sputum expectorations, although values for sputum were higher than for either group of saliva samples (p less than 0.025 and p less than 0.005, respecti ...19892663911
hyperammonemic encephalopathy: a complication associated with the prune belly syndrome.hyperammonemic encephalopathy in the urological patient is a rare but grave condition. in the pediatric urological population it has been associated with massively dilated upper tracts and urinary infection with urea-splitting organisms. we report 2 cases of hyperammonemic encephalopathy in association with the prune belly syndrome. both patients presented comatose with markedly elevated serum ammonia levels and proteus mirabilis urinary tract infection. intravenous antibiotics and catheter drai ...19892664223
[clinical studies on sulbactam/ampicillin in the field of pediatrics].during 8 months from october 1986 to may 1987, the clinical efficacy of sulbactam/ampicillin (sbt/abpc) was evaluated in 63 pediatric inpatients with various infections. clinical efficacies were evaluable in 58 patients among them (consisting of 2 patients with sepsis, 3 with tonsillitis, 12 with bronchitis, 6 with bronchopneumonia, 24 with pneumonia, 1 with phlegmon, 2 with lymphadenitis, 1 with impetigo and 7 with urinary tract infection) and were excellent in 40 patients and good in 17 with a ...19892664249
[susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates].susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ...19892664255
[evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae].m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ...19892665580
the relation between urinary tract infections and stone composition in renal stone formers.during a seven-year period (1975-1981) a total of 1325 patients hospitalized for stone disease were studied as to the occurrence of positive urine cultures. urinary stones from 535 surgically treated patients were analyzed with infrared spectrophotometry and the relationships between stone composition, level of surgery and bacteriological strains were studied. positive urinary cultures were found in 34% of the surgically treated patients and in 21% of those not operated upon. among the surgicall ...19892667113
proteus syndrome.the term proteus syndrome was coined in 1983 to describe a disorder of skeletal, hamartomatous, and other mesodermal malformations. the syndrome was named after the greek god proteus, whose name means "the polymorphous." clinical features of this new syndrome are currently being defined. including the case reported herein, we have found 34 patients with proteus syndrome described in the english literature. major clinical findings, defined as those findings seen in more than half of the cases, in ...19892667470
postpartum bacteriuria. a multicenter evaluation of different screening procedures and a controlled short-course treatment trial with amoxycillin.a total of 10,909 puerperal women from 6 different hospitals were screened for bacteriuria by culture of voided midstream urine (msu), and a significant growth was found in 881 patients (8.1%). in 731 cases the urine was reexamined by using suprapubic aspiration (spa), and in only 354 (48%) of the samples the diagnosis of bacteriuria was confirmed. the contamination rate of the msu samples varied from 46 to 69% between the different hospitals, indicating that in the postpartum period positive ms ...19892668060
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